Restriction map for
pFN21ASDA1456
(Circular) (Six-base) MAPSORT of: pFN21ASDA1456.seq Check: 462 from: 1 to: 5830
pFN21ASDA1456 5830 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3640 278
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3173 1937
Size: 1236 4594
Acc65I G'GTAC_C
Cuts at: 3151 3151
Size: 5830
AclI AA'CG_TT
Cuts at: 1563 3957 4330 1563
Size: 2394 373 3063
Fragments arranged by size:
3063 2394 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3893 5277 5545 1632
Size: 2261 1384 268 1917
Fragments arranged by size:
2261 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5633
AflIII A'CryG_T
Cuts at: 1203 4729 5706 1203
Size: 3526 977 1327
Fragments arranged by size:
3526 1327 977
AgeI A'CCGG_T
Cuts at: 4661 4661
Size: 5830
AhdI GACnn_n'nnGTC
Cuts at: 4559 4559
Size: 5830
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5830
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5830
AlwNI CAG_nnn'CTG
Cuts at: 3573 5145 3573
Size: 1572 4258
ApaI G_GGCC'C
Cuts at: 2251 2251
Size: 5830
ApaLI G'TGCA_C
Cuts at: 3887 5043 3887
Size: 1156 4674
ApoI r'AATT_y
Cuts at: 1493 2803 3139 3365 3517 5626 1493
Size: 1310 336 226 152 2109 1697
Fragments arranged by size:
2109 1697 1310 336 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4384 160
Size: 872 3352 1606
Fragments arranged by size:
3352 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
AvaI C'yCGr_G
Cuts at: 1870 1943 3155 1870
Size: 73 1212 4545
Fragments arranged by size:
4545 1212 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3076 3109 3076
Size: 33 5797
BamHI G'GATC_C
Cuts at: 3160 3527 3160
Size: 367 5463
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3151 4606 618
Size: 325 217 627 1364 1455 1842
Fragments arranged by size:
1842 1455 1364 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2251 3021 3054 3149 1391
Size: 860 770 33 95 4072
Fragments arranged by size:
4072 860 770 95 33
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4859
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4021 4055 1903
Size: 34 2084 34 3678
Fragments arranged by size:
3678 2084 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2300 3068 3722 4932 2300
Size: 768 654 1210 3198
Fragments arranged by size:
3198 1210 768 654
BclI T'GATC_A
Cuts at: 1548 2644 3076 1548
Size: 1096 432 4302
Fragments arranged by size:
4302 1096 432
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4441 136
Size: 107 122 71 631 605 2769 1525
Fragments arranged by size:
2769 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2522 5825 2522
Size: 3303 2527
BlpI GC'TnA_GC
Cuts at: 3247 3247
Size: 5830
Bme1580I G_kGCm'C
Cuts at: 1790 2251 3891 5047 1790
Size: 461 1640 1156 2573
Fragments arranged by size:
2573 1640 1156 461
BmgBI CAC'GTC
Cuts at: 1318 2227 1318
Size: 909 4921
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3561 4519 442
Size: 416 53 83 399 24 2144 958 1753
Fragments arranged by size:
2144 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2503 4490 1239
Size: 1264 1987 2579
Fragments arranged by size:
2579 1987 1264
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3017 3955 4820 5118 3017
Size: 938 865 298 3729
Fragments arranged by size:
3729 938 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2760 4493 882
Size: 617 1261 1733 2219
Fragments arranged by size:
2219 1733 1261 617
BsaAI yAC'GTr
Cuts at: 493 1204 3127 3621 493
Size: 711 1923 494 2702
Fragments arranged by size:
2702 1923 711 494
BsaBI GATnn'nnATC
Cuts at: 2865 5824 2865
Size: 2959 2871
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3637 4019 4654 275
Size: 53 83 186 936 2104 382 635 1451
Fragments arranged by size:
2104 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4263 4661 4935 5082 5493 4263
Size: 398 274 147 411 4600
Fragments arranged by size:
4600 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3029 3059 1178
Size: 30 165 30 1626 30 3949
Fragments arranged by size:
3949 1626 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2800 1186
Size: 1614 4216
BseYI C'CCAG_C
Cuts at: 2259 5033 5529 2259
Size: 2774 496 2560
Fragments arranged by size:
2774 2560 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5257
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3280 4041 5069 1540
Size: 275 186 1279 761 1028 2301
Fragments arranged by size:
2301 1279 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2740 3021 3054 3891 3976 5047 2740
Size: 281 33 837 85 1071 3523
Fragments arranged by size:
3523 1071 837 281 85 33
BsmI GAATG_Cn'
Cuts at: 3341 3434 3341
Size: 93 5737
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2251 2740 3021 3054 3149 3891 3976
Size: 399 461 489 281 33 95 742 85
Cuts at: 3976 5047 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 489 461 399 281 95
85 33
BspEI T'CCGG_A
Cuts at: 5493 5493
Size: 5830
BspHI T'CATG_A
Cuts at: 3719 5449 3719
Size: 1730 4100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2425 3185 844
Size: 1581 760 3489
Fragments arranged by size:
3489 1581 760
BsrBI CCG'CTC
Cuts at: 1394 1823 3717 1394
Size: 429 1894 3507
Fragments arranged by size:
3507 1894 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2196 3096 4325 4499 65
Size: 1183 948 900 1229 174 1396
Fragments arranged by size:
1396 1229 1183 948 900 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4474 4661 1700
Size: 110 2664 187 2869
Fragments arranged by size:
2869 2664 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5830
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5830
BssSI C'ACGA_G
Cuts at: 3890 4902 3890
Size: 1012 4818
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2227 794
Size: 1433 4397
BstBI TT'CG_AA
Cuts at: 2007 4634 2007
Size: 2627 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5830
BstXI CCAn_nnnn'nTGG
Cuts at: 2527 2527
Size: 5830
BstYI r'GATC_y
Cuts at: 1972 2522 2950 3160 3527 3912 3929 5381 5467
Size: 550 428 210 367 385 17 1452 86
Cuts at: 5467 5479 5825 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 550 428 385 367 346 210
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2237 513
Size: 552 957 215 4106
Fragments arranged by size:
4106 957 552 215
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5091
Fragments arranged by size:
5091 683 56
BtsI GCAGTG_nn'
Cuts at: 2306 3346 4140 4160 2306
Size: 1040 794 20 3976
Fragments arranged by size:
3976 1040 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5830
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5795
DraI TTT'AAA
Cuts at: 2371 2760 3134 2371
Size: 389 374 5067
Fragments arranged by size:
5067 389 374
DraIII CAC_nnn'GTG
Cuts at: 2404 2404
Size: 5830
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2631 4837 809
Size: 507 1315 2206 1802
Fragments arranged by size:
2206 1802 1315 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2142 3277 4166
Size: 54 1425 50 275 18 312 1135 889
Cuts at: 4166 5795 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1135 889 312 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3277 1537
Size: 275 1465 4090
Fragments arranged by size:
4090 1465 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2270 2288 3760 1353
Size: 917 18 1472 3423
Fragments arranged by size:
3423 1472 917 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4417 4791 4937 387
Size: 1064 138 2828 374 146 1280
Fragments arranged by size:
2828 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3019 3052 3019
Size: 33 5797
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2503 3893 4490 5277 5545 1239
Size: 393 871 1390 597 787 268 1524
Fragments arranged by size:
1524 1390 871 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2247 2248 2247
Size: 1 5829
EcoRI G'AATT_C
Cuts at: 3139 3517 3139
Size: 378 5452
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2246 2246
Size: 5830
FspI TGC'GCA
Cuts at: 4336 4336
Size: 5830
HaeII r_GCGC'y
Cuts at: 1678 4977 1678
Size: 3299 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2924 2956 3029 3061 4471
Size: 32 135 32 1352 32 73 32 1410
Cuts at: 4471 4503 4545 4577 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1410 1352 135 73 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3174 3420 669
Size: 988 281 1236 246 3079
Fragments arranged by size:
3079 1236 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5830
HpaI GTT'AAC
Cuts at: 3420 3420
Size: 5830
KpnI G_GTAC'C
Cuts at: 3155 3155
Size: 5830
MfeI C'AATT_G
Cuts at: 3429 3429
Size: 5830
MluI A'CGCG_T
Cuts at: 5706 5706
Size: 5830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4944 5128 1305
Size: 3639 184 2007
Fragments arranged by size:
3639 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2144 10
Size: 54 1425 343 312 3696
Fragments arranged by size:
3696 1425 343 312 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2760 3788 4147 4306 518
Size: 648 1594 1028 359 159 2042
Fragments arranged by size:
2042 1594 1028 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3246 3923 5071 5316 1606
Size: 1640 677 1148 245 2120
Fragments arranged by size:
2120 1640 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 2237 513
Size: 552 957 215 4106
Fragments arranged by size:
4106 957 552 215
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5830
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5830
NotI GC'GGCC_GC
Cuts at: 3277 3277
Size: 5830
NspI r_CATG'y
Cuts at: 1132 1229 3188 4733 1132
Size: 97 1959 1545 2229
Fragments arranged by size:
2229 1959 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5830
PciI A'CATG_T
Cuts at: 4729 4729
Size: 5830
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2404 1108
Size: 1296 4534
PfoI T'CCnGG_A
Cuts at: 2158 2158
Size: 5830
PmeI GTTT'AAAC
Cuts at: 3134 3134
Size: 5830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3899 3931 5412 5444 3899
Size: 32 1481 32 4285
Fragments arranged by size:
4285 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5830
PsiI TTA'TAA
Cuts at: 3400 3400
Size: 5830
PspOMI G'GGCC_C
Cuts at: 2247 2247
Size: 5830
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3049 3081 3049
Size: 32 5798
PstI C_TGCA'G
Cuts at: 830 2905 3026 3182 830
Size: 2075 121 156 3478
Fragments arranged by size:
3478 2075 156 121
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5830
SacI G_AGCT'C
Cuts at: 3021 3054 3021
Size: 33 5797
SalI G'TCGA_C
Cuts at: 1936 3172 1936
Size: 1236 4594
SapI GCTCTTCn'nnn_
Cuts at: 2270 2270
Size: 5830
SbfI CC_TGCA'GG
Cuts at: 3182 3182
Size: 5830
ScaI AGT'ACT
Cuts at: 4078 5790 4078
Size: 1712 4118
SfcI C'TryA_G
Cuts at: 826 1045 2901 3022 3178 4313 4994 5185 826
Size: 219 1856 121 156 1135 681 191 1471
Fragments arranged by size:
1856 1471 1135 681 219 191 156 121
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5830
SmaI CCC'GGG
Cuts at: 1872 3157 1872
Size: 1285 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3032 3934 4835 5097 820
Size: 197 926 1089 902 901 262 1553
Fragments arranged by size:
1553 1089 926 902 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3127 3621 493
Size: 2634 494 2702
Fragments arranged by size:
2702 2634 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5830
SphI G_CATG'C
Cuts at: 1132 3188 1132
Size: 2056 3774
SspI AAT'ATT
Cuts at: 5 52 2446 2886 3754 5
Size: 47 2394 440 868 2081
Fragments arranged by size:
2394 2081 868 440 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5830
StyI C'CwwG_G
Cuts at: 513 1065 2022 2237 3269 513
Size: 552 957 215 1032 3074
Fragments arranged by size:
3074 1032 957 552 215
SwaI ATTT'AAAT
Cuts at: 2760 2760
Size: 5830
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3857 3874 4027 4212 4657 325
Size: 1255 2277 17 153 185 445 1498
Fragments arranged by size:
2277 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4076 5788 96
Size: 274 80 33 51 1443 2099 1712 138
Fragments arranged by size:
2099 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5790 5822 5790
Size: 32 5798
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5830
XbaI T'CTAG_A
Cuts at: 2635 3166 2635
Size: 531 5299
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5830
XmaI C'CCGG_G
Cuts at: 1870 3155 1870
Size: 1285 4545
XmnI GAAnn'nnTTC
Cuts at: 2896 3959 2896
Size: 1063 4767
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3638 276
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PsrI PstI PvuI SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspXI PvuII RsrII SacII SanDI
SexAI SfiI SfoI SrfI XcmI