Restriction map for
pFN21ASDA1453
(Circular) (Six-base) MAPSORT of: pFN21ASDA1453.seq Check: 5944 from: 1 to: 8092
pFN21ASDA1453 8092 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2420 4258 4711 2420
Size: 1838 453 5801
Fragments arranged by size:
5801 1838 453
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2835 4337 5902 278
Size: 53 83 186 936 1299 1502 1565 2468
Fragments arranged by size:
2468 1565 1502 1299 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5435 1937
Size: 3498 4594
Acc65I G'GTAC_C
Cuts at: 3204 5413 3204
Size: 2209 5883
AclI AA'CG_TT
Cuts at: 1563 6219 6592 1563
Size: 4656 373 3063
Fragments arranged by size:
4656 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3007 3489 3536 3830 4037 4270 5303 6155
Size: 1375 482 47 294 207 233 1033 852
Cuts at: 6155 7539 7807 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1375 1033 852 482 294 268
233 207 47
AfeI AGC'GCT
Cuts at: 2963 3829 2963
Size: 866 7226
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7895
AflIII A'CryG_T
Cuts at: 1203 2286 2776 2922 6991 7968 1203
Size: 1083 490 146 4069 977 1327
Fragments arranged by size:
4069 1327 1083 977 490 146
AgeI A'CCGG_T
Cuts at: 6923 6923
Size: 8092
AhdI GACnn_n'nnGTC
Cuts at: 4396 6821 4396
Size: 2425 5667
AleI CACnn'nnGTG
Cuts at: 1166 2244 1166
Size: 1078 7014
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8092
AlwNI CAG_nnn'CTG
Cuts at: 3679 3791 4489 4995 5101 5835 7407 3679
Size: 112 698 506 106 734 1572 4364
Fragments arranged by size:
4364 1572 734 698 506 112 106
ApaLI G'TGCA_C
Cuts at: 3137 6149 7305 3137
Size: 3012 1156 3924
Fragments arranged by size:
3924 3012 1156
ApoI r'AATT_y
Cuts at: 1493 2751 3062 3288 5273 5401 5627 5779 7888
Size: 1258 311 226 1985 128 226 152 2109
Cuts at: 7888 1493
Size: 1697
Fragments arranged by size:
2109 1985 1697 1258 311 226 226 152
128
AseI AT'TA_AT
Cuts at: 160 1032 6646 160
Size: 872 5614 1606
Fragments arranged by size:
5614 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8092
AvaI C'yCGr_G
Cuts at: 1870 1943 3142 3311 3358 3553 4982 5417 1870
Size: 73 1199 169 47 195 1429 435 4545
Fragments arranged by size:
4545 1429 1199 435 195 169 73 47
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3188 3221 3188
Size: 33 8059
BamHI G'GATC_C
Cuts at: 5422 5789 5422
Size: 367 7725
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3204 5413 6868 618
Size: 325 217 627 1417 2209 1455 1842
Fragments arranged by size:
2209 1842 1455 1417 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3734 3851 4434 4445 4496 4637 5411 1391
Size: 2343 117 583 11 51 141 774 4072
Fragments arranged by size:
4072 2343 774 583 141 117 51 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2365 5196 928
Size: 971 466 2831 3824
Fragments arranged by size:
3824 2831 971 466
BbvCI CC'TCA_GC
Cuts at: 5372 5372
Size: 8092
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2848 2882 4354 4388 6283 6317 1903
Size: 34 911 34 1472 34 1895 34 3678
Fragments arranged by size:
3678 1895 1472 911 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5984 7194 5984
Size: 1210 6882
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8092
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2700 4106 4486
Size: 107 122 71 631 605 1028 1406 380
Cuts at: 4486 4535 6703 136
Size: 49 2168 1525
Fragments arranged by size:
2168 1525 1406 1028 631 605 380 122
107 71 49
BglII A'GATC_T
Cuts at: 3710 8087 3710
Size: 4377 3715
BlpI GC'TnA_GC
Cuts at: 5042 5509 5042
Size: 467 7625
Bme1580I G_kGCm'C
Cuts at: 1790 3141 3168 3486 6153 7309 1790
Size: 1351 27 318 2667 1156 2573
Fragments arranged by size:
2667 2573 1351 1156 318 27
BmgBI CAC'GTC
Cuts at: 1318 4503 1318
Size: 3185 4907
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2102 5239 5823
Size: 416 53 83 399 24 685 3137 584
Cuts at: 5823 6781 442
Size: 958 1753
Fragments arranged by size:
3137 1753 958 685 584 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8092
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2783 2783
Size: 8092
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2201 2861 3310 3835 3844 6752 1239
Size: 962 660 449 525 9 2908 2579
Fragments arranged by size:
2908 2579 962 660 525 449 9
Bpu10I CC'TnA_GC
Cuts at: 3672 3771 4031 5372 3672
Size: 99 260 1341 6392
Fragments arranged by size:
6392 1341 260 99
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2990 6217 7082 7380 2990
Size: 3227 865 298 3702
Fragments arranged by size:
3702 3227 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2592 5207 6755 882
Size: 617 1093 2615 1548 2219
Fragments arranged by size:
2615 2219 1548 1093 617
BsaAI yAC'GTr
Cuts at: 493 1204 2289 3181 3271 5389 5883 493
Size: 711 1085 892 90 2118 494 2702
Fragments arranged by size:
2702 2118 1085 892 711 494 90
BsaBI GATnn'nnATC
Cuts at: 4854 4935 8086 4854
Size: 81 3151 4860
Fragments arranged by size:
4860 3151 81
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2676 2832 4334 5899
Size: 53 83 186 936 1143 156 1502 1565
Cuts at: 5899 6281 6916 275
Size: 382 635 1451
Fragments arranged by size:
1565 1502 1451 1143 936 635 382 186
156 83 53
BsaWI w'CCGG_w
Cuts at: 3058 6525 6923 7197 7344 7755 3058
Size: 3467 398 274 147 411 3395
Fragments arranged by size:
3467 3395 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2336 2366 2459 2489 3759
Size: 30 165 30 933 30 93 30 1270
Cuts at: 3759 3789 4051 4081 4522 4552 1178
Size: 30 262 30 441 30 4718
Fragments arranged by size:
4718 1270 933 441 262 165 93 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2101 3328 3331 3633 4059 4345 4783 5305
Size: 915 1227 3 302 426 286 438 522
Cuts at: 5305 1186
Size: 3973
Fragments arranged by size:
3973 1227 915 522 438 426 302 286
3
BseYI C'CCAG_C
Cuts at: 2314 4489 5353 7295 7791 2314
Size: 2175 864 1942 496 2615
Fragments arranged by size:
2615 2175 1942 864 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4793 1163
Size: 573 3057 4462
Fragments arranged by size:
4462 3057 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2065 3355 4131 5542 6303 7331
Size: 275 186 64 1290 776 1411 761 1028
Cuts at: 7331 1540
Size: 2301
Fragments arranged by size:
2301 1411 1290 1028 776 761 275 186
64
BsiHKAI G_wGCw'C
Cuts at: 2286 2789 2875 3141 3873 6153 6238 7309 2286
Size: 503 86 266 732 2280 85 1071 3069
Fragments arranged by size:
3069 2280 1071 732 503 266 86 85
BsmI GAATG_Cn'
Cuts at: 2920 3534 5603 5696 2920
Size: 614 2069 93 5316
Fragments arranged by size:
5316 2069 614 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2590 2809 4493 2590
Size: 219 1684 6189
Fragments arranged by size:
6189 1684 219
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2286 2789 2875 3141 3168 3486 3734
Size: 399 496 503 86 266 27 318 248
Cuts at: 3734 3851 3873 4434 4445 4496 4637 5411 6153
Size: 117 22 561 11 51 141 774 742
Cuts at: 6153 6238 7309 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 774 742 561 503 496 399
318 266 248 141 117 86 85 51
27 22 11
BspEI T'CCGG_A
Cuts at: 3058 7755 3058
Size: 4697 3395
BspHI T'CATG_A
Cuts at: 5981 7711 5981
Size: 1730 6362
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2420 2478 4258 4711 5447 844
Size: 1576 58 1780 453 736 3489
Fragments arranged by size:
3489 1780 1576 736 453 58
BsrBI CCG'CTC
Cuts at: 1394 1823 2368 3923 5077 5979 1394
Size: 429 545 1555 1154 902 3507
Fragments arranged by size:
3507 1555 1154 902 545 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3199 3403 4141 4301 6587 6761 65
Size: 1183 1951 204 738 160 2286 174 1396
Fragments arranged by size:
2286 1951 1396 1183 738 204 174 160
BsrFI r'CCGG_y
Cuts at: 1700 1810 2260 4130 6736 6923 1700
Size: 110 450 1870 2606 187 2869
Fragments arranged by size:
2869 2606 1870 450 187 110
BsrGI T'GTAC_A
Cuts at: 96 4641 96
Size: 4545 3547
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8092
BssSI C'ACGA_G
Cuts at: 6152 7164 6152
Size: 1012 7080
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2329 2412 5363 794
Size: 1535 83 2951 3523
Fragments arranged by size:
3523 2951 1535 83
BstBI TT'CG_AA
Cuts at: 2007 6896 2007
Size: 4889 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8092
BstXI CCAn_nnnn'nTGG
Cuts at: 2246 2402 3098 4087 2246
Size: 156 696 989 6251
Fragments arranged by size:
6251 989 696 156
BstYI r'GATC_y
Cuts at: 1972 2844 3710 5422 5789 6174 6191 7643 7729
Size: 872 866 1712 367 385 17 1452 86
Cuts at: 7729 7741 8087 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1712 1452 872 866 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4377 4377
Size: 8092
BtgI C'CryG_G
Cuts at: 513 1065 2340 4343 4543 4842 4866 5217 513
Size: 552 1275 2003 200 299 24 351 3388
Fragments arranged by size:
3388 2003 1275 552 351 299 200 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7353
Fragments arranged by size:
7353 683 56
BtsI GCAGTG_nn'
Cuts at: 2284 3789 4530 5608 6402 6422 2284
Size: 1505 741 1078 794 20 3954
Fragments arranged by size:
3954 1505 1078 794 741 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8092
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4395 4430 529
Size: 35 3831 35 4191
Fragments arranged by size:
4191 3831 35 35
DraI TTT'AAA
Cuts at: 5396 5396
Size: 8092
DraIII CAC_nnn'GTG
Cuts at: 4119 4119
Size: 8092
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7099 809
Size: 507 5783 1802
Fragments arranged by size:
5783 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2062 2262 3352
Size: 54 1425 50 275 18 232 200 1090
Cuts at: 3352 3876 3967 4714 4741 5539 6428 8057 8
Size: 524 91 747 27 798 889 1629 43
Fragments arranged by size:
1629 1425 1090 889 798 747 524 275
232 200 91 54 50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 2062 3352 5539 1537
Size: 275 250 1290 2187 4090
Fragments arranged by size:
4090 2187 1290 275 250
EarI CTCTTCn'nnn_
Cuts at: 1353 3465 4448 4544 4550 4866 4920 5232 5282
Size: 2112 983 96 6 316 54 312 50
Cuts at: 5282 6022 1353
Size: 740 3423
Fragments arranged by size:
3423 2112 983 740 316 312 96 54
50 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6679 7053 7199 387
Size: 1064 138 5090 374 146 1280
Fragments arranged by size:
5090 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2201 2861 3007 3310 3489 3536 3830
Size: 393 569 660 146 303 179 47 294
Cuts at: 3830 3835 3844 4037 4270 5303 6155 6752 7539
Size: 5 9 193 233 1033 852 597 787
Cuts at: 7539 7807 1239
Size: 268 1524
Fragments arranged by size:
1524 1033 852 787 660 597 569 393
303 294 268 233 193 179 146 47
9 5
EcoNI CCTnn'n_nnAGG
Cuts at: 4604 4811 4604
Size: 207 7885
EcoO109I rG'GnC_Cy
Cuts at: 2050 2125 3316 3558 3898 4390 4816 5050 2050
Size: 75 1191 242 340 492 426 234 5092
Fragments arranged by size:
5092 1191 492 426 340 242 234 75
EcoRI G'AATT_C
Cuts at: 3062 3288 5401 5779 3062
Size: 226 2113 378 5375
Fragments arranged by size:
5375 2113 378 226
FspI TGC'GCA
Cuts at: 4999 6598 4999
Size: 1599 6493
HaeII r_GCGC'y
Cuts at: 1678 2965 3831 4373 4576 4599 7239 1678
Size: 1287 866 542 203 23 2640 2531
Fragments arranged by size:
2640 2531 1287 866 542 203 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2336 2368 3358 3390 4382
Size: 32 135 32 764 32 990 32 992
Cuts at: 4382 4414 5066 5098 6733 6765 6807 6839 1373
Size: 32 652 32 1635 32 42 32 2626
Fragments arranged by size:
2626 1635 992 990 764 652 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4384 5436 5682 669
Size: 988 281 2446 1052 246 3079
Fragments arranged by size:
3079 2446 1052 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2099 2509 5124 748
Size: 1351 410 2615 3716
Fragments arranged by size:
3716 2615 1351 410
HpaI GTT'AAC
Cuts at: 5682 5682
Size: 8092
KpnI G_GTAC'C
Cuts at: 3208 5417 3208
Size: 2209 5883
MfeI C'AATT_G
Cuts at: 5691 5691
Size: 8092
MluI A'CGCG_T
Cuts at: 2776 7968 2776
Size: 5192 2900
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2694 3009 4194 7206 7390 1305
Size: 1389 315 1185 3012 184 2007
Fragments arranged by size:
3012 2007 1389 1185 315 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3969 4716 10
Size: 54 1425 343 2137 747 3386
Fragments arranged by size:
3386 2137 1425 747 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2244 2289 3593 6050 6409 6568 518
Size: 648 1078 45 1304 2457 359 159 2042
Fragments arranged by size:
2457 2042 1304 1078 648 359 159 45
MspA1I CmG'CkG
Cuts at: 1606 2179 2496 2565 3027 3153 3965 4589 4666
Size: 573 317 69 462 126 812 624 77
Cuts at: 4666 4673 4833 4915 4995 5508 6185 7333 7578
Size: 7 160 82 80 513 677 1148 245
Cuts at: 7578 1606
Size: 2120
Fragments arranged by size:
2120 1148 812 677 624 573 513 462
317 245 160 126 82 80 77 69
7
NcoI C'CATG_G
Cuts at: 513 1065 4842 4866 513
Size: 552 3777 24 3739
Fragments arranged by size:
3777 3739 552 24
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8092
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8092
NotI GC'GGCC_GC
Cuts at: 5539 5539
Size: 8092
NspI r_CATG'y
Cuts at: 1132 1229 2489 2521 2926 3598 5450 6995 1132
Size: 97 1260 32 405 672 1852 1545 2229
Fragments arranged by size:
2229 1852 1545 1260 672 405 97 32
PasI CC'CwG_GG
Cuts at: 1348 3508 4328 1348
Size: 2160 820 5112
Fragments arranged by size:
5112 2160 820
PciI A'CATG_T
Cuts at: 2922 6991 2922
Size: 4069 4023
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3422 4723 1108
Size: 2314 1301 4477
Fragments arranged by size:
4477 2314 1301
PfoI T'CCnGG_A
Cuts at: 3326 3491 5094 3326
Size: 165 1603 6324
Fragments arranged by size:
6324 1603 165
PmeI GTTT'AAAC
Cuts at: 5396 5396
Size: 8092
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6161 6193 7674 7706 6161
Size: 32 1481 32 6547
Fragments arranged by size:
6547 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2125 3558 4390 4816 2125
Size: 1433 832 426 5401
Fragments arranged by size:
5401 1433 832 426
PshAI GACnn'nnGTC
Cuts at: 1613 4143 1613
Size: 2530 5562
PsiI TTA'TAA
Cuts at: 5662 5662
Size: 8092
PstI C_TGCA'G
Cuts at: 830 4605 5444 830
Size: 3775 839 3478
Fragments arranged by size:
3775 3478 839
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8092
PvuII CAG'CTG
Cuts at: 2179 2496 3153 4589 4666 4833 4995 2179
Size: 317 657 1436 77 167 162 5276
Fragments arranged by size:
5276 1436 657 317 167 162 77
SalI G'TCGA_C
Cuts at: 1936 5434 1936
Size: 3498 4594
SanDI GG'GwC_CC
Cuts at: 3558 3558
Size: 8092
SbfI CC_TGCA'GG
Cuts at: 4605 5444 4605
Size: 839 7253
ScaI AGT'ACT
Cuts at: 6340 8052 6340
Size: 1712 6380
SfcI C'TryA_G
Cuts at: 826 1045 3148 3783 4601 5440 6575 7256 7447
Size: 219 2103 635 818 839 1135 681 191
Cuts at: 7447 826
Size: 1471
Fragments arranged by size:
2103 1471 1135 839 818 681 635 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8092
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8092
SmaI CCC'GGG
Cuts at: 1872 5419 1872
Size: 3547 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3005 6196 7097 7359 820
Size: 197 926 1062 3191 901 262 1553
Fragments arranged by size:
3191 1553 1062 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5389 5883 493
Size: 4896 494 2702
Fragments arranged by size:
4896 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8092
SphI G_CATG'C
Cuts at: 1132 2489 2521 5450 1132
Size: 1357 32 2929 3774
Fragments arranged by size:
3774 2929 1357 32
SspI AAT'ATT
Cuts at: 5 52 2863 6016 5
Size: 47 2811 3153 2081
Fragments arranged by size:
3153 2811 2081 47
StuI AGG'CCT
Cuts at: 1517 2186 2552 3266 1517
Size: 669 366 714 6343
Fragments arranged by size:
6343 714 669 366
StyI C'CwwG_G
Cuts at: 513 1065 2401 2478 2739 3042 3222 3894 4842
Size: 552 1336 77 261 303 180 672 948
Cuts at: 4842 4866 4880 4949 5531 513
Size: 24 14 69 582 3074
Fragments arranged by size:
3074 1336 948 672 582 552 303 261
180 77 69 24 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3601 4193 4835 5262 5367 6119 6136
Size: 1255 2021 592 642 427 105 752 17
Cuts at: 6136 6289 6474 6919 325
Size: 153 185 445 1498
Fragments arranged by size:
2021 1498 1255 752 642 592 445 427
185 153 105 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4641 6338 8050
Size: 274 80 33 51 1443 2664 1697 1712
Cuts at: 8050 96
Size: 138
Fragments arranged by size:
2664 1712 1697 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2966 2998 3612 3644 3759 3791 4051 4083 4522
Size: 32 614 32 115 32 260 32 439
Cuts at: 4522 4554 8052 8084 2966
Size: 32 3498 32 2974
Fragments arranged by size:
3498 2974 614 439 260 115 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2309 1316
Size: 993 7099
XbaI T'CTAG_A
Cuts at: 5428 5428
Size: 8092
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 2753 2029
Size: 724 7368
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8092
XmaI C'CCGG_G
Cuts at: 1870 5417 1870
Size: 3547 4545
XmnI GAAnn'nnTTC
Cuts at: 6221 6221
Size: 8092
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2833 4335 5900 276
Size: 53 83 186 936 1299 1502 1565 2468
Fragments arranged by size:
2468 1565 1502 1299 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbeI BfrBI BsiWI BstZ17I
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SapI SexAI SfiI SfoI SrfI
SwaI