Restriction map for
pFN21ASDA1443
(Circular) (Six-base) MAPSORT of: pFN21ASDA1443.seq Check: 6753 from: 1 to: 6376
pFN21ASDA1443 6376 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2238 3493 2238
Size: 1255 5121
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4186 278
Size: 53 83 186 936 2650 2468
Fragments arranged by size:
2650 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2403 3719 1937
Size: 466 1316 4594
Fragments arranged by size:
4594 1316 466
Acc65I G'GTAC_C
Cuts at: 3697 3697
Size: 6376
AclI AA'CG_TT
Cuts at: 1563 4503 4876 1563
Size: 2940 373 3063
Fragments arranged by size:
3063 2940 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2091 2227 2391 4439 5823 6091 1632
Size: 459 136 164 2048 1384 268 1917
Fragments arranged by size:
2048 1917 1384 459 268 164 136
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6179
AflIII A'CryG_T
Cuts at: 1203 5275 6252 1203
Size: 4072 977 1327
Fragments arranged by size:
4072 1327 977
AgeI A'CCGG_T
Cuts at: 5207 5207
Size: 6376
AhdI GACnn_n'nnGTC
Cuts at: 5105 5105
Size: 6376
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6376
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6376
AlwNI CAG_nnn'CTG
Cuts at: 2905 3296 3508 4119 5691 2905
Size: 391 212 611 1572 3590
Fragments arranged by size:
3590 1572 611 391 212
ApaLI G'TGCA_C
Cuts at: 4433 5589 4433
Size: 1156 5220
ApoI r'AATT_y
Cuts at: 1493 3685 3911 4063 6172 1493
Size: 2192 226 152 2109 1697
Fragments arranged by size:
2192 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2825 4930 160
Size: 872 1793 2105 1606
Fragments arranged by size:
2105 1793 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6376
AvaI C'yCGr_G
Cuts at: 1870 1943 2048 2388 2394 2799 3701 1870
Size: 73 105 340 6 405 902 4545
Fragments arranged by size:
4545 902 405 340 105 73 6
BamHI G'GATC_C
Cuts at: 3706 4073 3706
Size: 367 6009
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2090 2476 3299 3697 5152
Size: 325 217 627 303 386 823 398 1455
Cuts at: 5152 618
Size: 1842
Fragments arranged by size:
1842 1455 823 627 398 386 325 303
217
BanII G_rGCy'C
Cuts at: 1391 2145 2395 2586 3695 1391
Size: 754 250 191 1109 4072
Fragments arranged by size:
4072 1109 754 250 191
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5405
BbvCI CC'TCA_GC
Cuts at: 3534 3543 3534
Size: 9 6367
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4567 4601 1903
Size: 34 2630 34 3678
Fragments arranged by size:
3678 2630 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4268 5478 4268
Size: 1210 5166
BclI T'GATC_A
Cuts at: 1548 3657 1548
Size: 2109 4267
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2050 3022 4987
Size: 107 122 71 631 605 378 972 1965
Cuts at: 4987 136
Size: 1525
Fragments arranged by size:
1965 1525 972 631 605 378 122 107
71
BglII A'GATC_T
Cuts at: 6371 6371
Size: 6376
BlpI GC'TnA_GC
Cuts at: 3491 3793 3491
Size: 302 6074
Bme1580I G_kGCm'C
Cuts at: 1790 2079 3166 3304 4437 5593 1790
Size: 289 1087 138 1133 1156 2573
Fragments arranged by size:
2573 1156 1133 1087 289 138
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6376
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2970 3439 4107
Size: 416 53 83 399 24 1553 469 668
Cuts at: 4107 5065 442
Size: 958 1753
Fragments arranged by size:
1753 1553 958 668 469 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6376
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2602 2602
Size: 6376
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2472 2484 2924 5036 1239
Size: 1233 12 440 2112 2579
Fragments arranged by size:
2579 2112 1233 440 12
Bpu10I CC'TnA_GC
Cuts at: 3534 3543 3534
Size: 9 6367
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4501 5366 5664 4501
Size: 865 298 5213
Fragments arranged by size:
5213 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2126 5039 882
Size: 617 627 2913 2219
Fragments arranged by size:
2913 2219 627 617
BsaAI yAC'GTr
Cuts at: 493 1204 3673 4167 493
Size: 711 2469 494 2702
Fragments arranged by size:
2702 2469 711 494
BsaBI GATnn'nnATC
Cuts at: 6370 6370
Size: 6376
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4183 4565 5200 275
Size: 53 83 186 936 2650 382 635 1451
Fragments arranged by size:
2650 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3403 4809 5207 5481 5628 6039 3403
Size: 1406 398 274 147 411 3740
Fragments arranged by size:
3740 1406 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2083 2113 1178
Size: 30 165 30 680 30 5441
Fragments arranged by size:
5441 680 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2183 2472 2475 2486 2594 2861 3593 3596
Size: 997 289 3 11 108 267 732 3
Cuts at: 3596 3629 3632 3635 3638 1186
Size: 33 3 3 3 3924
Fragments arranged by size:
3924 997 732 289 267 108 33 11
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2034 2261 2337 2787 3319 3498 5579 6075 2034
Size: 227 76 450 532 179 2081 496 2335
Fragments arranged by size:
2335 2081 532 496 450 227 179 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5803
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3826 4587 5615 1540
Size: 275 186 1825 761 1028 2301
Fragments arranged by size:
2301 1825 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4437 4522 5593 4437
Size: 85 1071 5220
Fragments arranged by size:
5220 1071 85
BsmI GAATG_Cn'
Cuts at: 2657 3887 3980 2657
Size: 1230 93 5053
Fragments arranged by size:
5053 1230 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2145 2395 2586 3166 3304 3695
Size: 399 289 66 250 191 580 138 391
Cuts at: 3695 4437 4522 5593 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 580 399 391 289 250
191 138 85 66
BspEI T'CCGG_A
Cuts at: 6039 6039
Size: 6376
BspHI T'CATG_A
Cuts at: 4265 5995 4265
Size: 1730 4646
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2238 3493 3731 844
Size: 1394 1255 238 3489
Fragments arranged by size:
3489 1394 1255 238
BsrBI CCG'CTC
Cuts at: 1394 1823 3351 4263 1394
Size: 429 1528 912 3507
Fragments arranged by size:
3507 1528 912 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2227 2274 4871 5045 65
Size: 1183 979 47 2597 174 1396
Fragments arranged by size:
2597 1396 1183 979 174 47
BsrFI r'CCGG_y
Cuts at: 1700 1810 5020 5207 1700
Size: 110 3210 187 2869
Fragments arranged by size:
3210 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6376
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6376
BssSI C'ACGA_G
Cuts at: 4436 5448 4436
Size: 1012 5364
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2276 2828 794
Size: 1482 552 4342
Fragments arranged by size:
4342 1482 552
BstBI TT'CG_AA
Cuts at: 2007 5180 2007
Size: 3173 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6376
BstXI CCAn_nnnn'nTGG
Cuts at: 2744 2744
Size: 6376
BstYI r'GATC_y
Cuts at: 1972 3706 4073 4458 4475 5927 6013 6025 6371
Size: 1734 367 385 17 1452 86 12 346
Cuts at: 6371 1972
Size: 1977
Fragments arranged by size:
1977 1734 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2577 2577
Size: 6376
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4867
Fragments arranged by size:
4867 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5637
Fragments arranged by size:
5637 683 56
BtsI GCAGTG_nn'
Cuts at: 2600 2656 2704 3892 4686 4706 2600
Size: 56 48 1188 794 20 4270
Fragments arranged by size:
4270 1188 794 56 48 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6376
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3254 3289 529
Size: 35 2690 35 3616
Fragments arranged by size:
3616 2690 35 35
DraI TTT'AAA
Cuts at: 3680 3680
Size: 6376
DraIII CAC_nnn'GTG
Cuts at: 2978 3170 2978
Size: 192 6184
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5383 809
Size: 507 4067 1802
Fragments arranged by size:
4067 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3823 4712 6341
Size: 54 1425 50 275 18 1993 889 1629
Cuts at: 6341 8
Size: 43
Fragments arranged by size:
1993 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3823 1537
Size: 275 2011 4090
Fragments arranged by size:
4090 2011 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2376 2878 2893 3564 3570 3606 4306 1353
Size: 1023 502 15 671 6 36 700 3423
Fragments arranged by size:
3423 1023 700 671 502 36 15 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4963 5337 5483 387
Size: 1064 138 3374 374 146 1280
Fragments arranged by size:
3374 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2091 2227 2391 2472 2484 2924 4439
Size: 393 459 136 164 81 12 440 1515
Cuts at: 4439 5036 5823 6091 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1515 787 597 459 440 393 268
164 136 81 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2708 3421 2708
Size: 713 5663
EcoO109I rG'GnC_Cy
Cuts at: 2525 2729 2525
Size: 204 6172
EcoRI G'AATT_C
Cuts at: 3685 4063 3685
Size: 378 5998
EcoRV GAT'ATC
Cuts at: 3463 3463
Size: 6376
FspI TGC'GCA
Cuts at: 4882 4882
Size: 6376
HaeII r_GCGC'y
Cuts at: 1678 2344 5523 1678
Size: 666 3179 2531
Fragments arranged by size:
3179 2531 666
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2840 2872 3364 3396 5017
Size: 32 135 32 1268 32 492 32 1621
Cuts at: 5017 5049 5091 5123 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1621 1268 492 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3720 3966 669
Size: 988 281 1782 246 3079
Fragments arranged by size:
3079 1782 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6376
HpaI GTT'AAC
Cuts at: 3966 3966
Size: 6376
KpnI G_GTAC'C
Cuts at: 3701 3701
Size: 6376
MfeI C'AATT_G
Cuts at: 2817 3470 3975 2817
Size: 653 505 5218
Fragments arranged by size:
5218 653 505
MluI A'CGCG_T
Cuts at: 6252 6252
Size: 6376
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2555 5490 5674 1305
Size: 1250 2935 184 2007
Fragments arranged by size:
2935 2007 1250 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4554
Fragments arranged by size:
4554 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4334 4693 4852 518
Size: 648 3168 359 159 2042
Fragments arranged by size:
3168 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2118 2138 2349 3124 3542 3792 4469 5617
Size: 512 20 211 775 418 250 677 1148
Cuts at: 5617 5862 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 775 677 512 418 250 245
211 20
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4867
Fragments arranged by size:
4867 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6376
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6376
NotI GC'GGCC_GC
Cuts at: 3823 3823
Size: 6376
NspI r_CATG'y
Cuts at: 1132 1229 3734 5279 1132
Size: 97 2505 1545 2229
Fragments arranged by size:
2505 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6376
PciI A'CATG_T
Cuts at: 5275 5275
Size: 6376
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2905 1108
Size: 1797 4579
PfoI T'CCnGG_A
Cuts at: 2762 2762
Size: 6376
PmeI GTTT'AAAC
Cuts at: 3680 3680
Size: 6376
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2217 2249 4445 4477 5958 5990 2217
Size: 32 2196 32 1481 32 2603
Fragments arranged by size:
2603 2196 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2525 2729 2525
Size: 204 6172
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6376
PsiI TTA'TAA
Cuts at: 3946 3946
Size: 6376
PspXI vC'TCGA_Gb
Cuts at: 2388 2388
Size: 6376
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2973 3005 2973
Size: 32 6344
PstI C_TGCA'G
Cuts at: 830 3728 830
Size: 2898 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6376
PvuII CAG'CTG
Cuts at: 3124 3542 3124
Size: 418 5958
SalI G'TCGA_C
Cuts at: 1936 3718 1936
Size: 1782 4594
SanDI GG'GwC_CC
Cuts at: 2729 2729
Size: 6376
SbfI CC_TGCA'GG
Cuts at: 3728 3728
Size: 6376
ScaI AGT'ACT
Cuts at: 4624 6336 4624
Size: 1712 4664
SexAI A'CCwGG_T
Cuts at: 3043 3295 3043
Size: 252 6124
SfcI C'TryA_G
Cuts at: 826 1045 2174 3724 4859 5540 5731 826
Size: 219 1129 1550 1135 681 191 1471
Fragments arranged by size:
1550 1471 1135 1129 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6376
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6376
SmaI CCC'GGG
Cuts at: 1872 2050 3703 1872
Size: 178 1653 4545
Fragments arranged by size:
4545 1653 178
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2388 4480 5381 5643 820
Size: 197 926 445 2092 901 262 1553
Fragments arranged by size:
2092 1553 926 901 445 262 197
SnaBI TAC'GTA
Cuts at: 493 3673 4167 493
Size: 3180 494 2702
Fragments arranged by size:
3180 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6376
SphI G_CATG'C
Cuts at: 1132 3734 1132
Size: 2602 3774
SrfI GCCC'GGGC
Cuts at: 2050 2050
Size: 6376
SspI AAT'ATT
Cuts at: 5 52 4300 5
Size: 47 4248 2081
Fragments arranged by size:
4248 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6376
StyI C'CwwG_G
Cuts at: 513 1065 2022 3019 3417 3815 513
Size: 552 957 997 398 398 3074
Fragments arranged by size:
3074 997 957 552 398 398
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2989 3512 4403 4420 4573 4758 5203
Size: 1255 1409 523 891 17 153 185 445
Cuts at: 5203 325
Size: 1498
Fragments arranged by size:
1498 1409 1255 891 523 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4622 6334 96
Size: 274 80 33 51 1443 2645 1712 138
Fragments arranged by size:
2645 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2083 2115 6336 6368 2083
Size: 32 4221 32 2091
Fragments arranged by size:
4221 2091 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6376
XbaI T'CTAG_A
Cuts at: 3559 3712 3559
Size: 153 6223
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3121 3121
Size: 6376
XhoI C'TCGA_G
Cuts at: 1943 2388 1943
Size: 445 5931
XmaI C'CCGG_G
Cuts at: 1870 2048 3701 1870
Size: 178 1653 4545
Fragments arranged by size:
4545 1653 178
XmnI GAAnn'nnTTC
Cuts at: 4505 4505
Size: 6376
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4184 276
Size: 53 83 186 936 2650 2468
Fragments arranged by size:
2650 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI
PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I EcoICRI FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
RsrII SacI SacII SapI SfiI SfoI SwaI