Restriction map for
pFN21ASDA1442
(Circular) (Six-base) MAPSORT of: pFN21ASDA1442.seq Check: 9836 from: 1 to: 6349
pFN21ASDA1442 6349 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4159 278
Size: 53 83 186 936 2623 2468
Fragments arranged by size:
2623 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3021 3692 1937
Size: 1084 671 4594
Fragments arranged by size:
4594 1084 671
Acc65I G'GTAC_C
Cuts at: 3670 3670
Size: 6349
AclI AA'CG_TT
Cuts at: 1563 4476 4849 1563
Size: 2913 373 3063
Fragments arranged by size:
3063 2913 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2075 2630 3149 3215 3292 4412 5796 6064
Size: 443 555 519 66 77 1120 1384 268
Cuts at: 6064 1632
Size: 1917
Fragments arranged by size:
1917 1384 1120 555 519 443 268 77
66
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6152
AflIII A'CryG_T
Cuts at: 1203 2683 2706 5248 6225 1203
Size: 1480 23 2542 977 1327
Fragments arranged by size:
2542 1480 1327 977 23
AgeI A'CCGG_T
Cuts at: 5180 5180
Size: 6349
AhdI GACnn_n'nnGTC
Cuts at: 2413 5078 2413
Size: 2665 3684
AleI CACnn'nnGTG
Cuts at: 1166 2666 1166
Size: 1500 4849
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6349
AlwNI CAG_nnn'CTG
Cuts at: 3187 4092 5664 3187
Size: 905 1572 3872
Fragments arranged by size:
3872 1572 905
ApaI G_GGCC'C
Cuts at: 3354 3354
Size: 6349
ApaLI G'TGCA_C
Cuts at: 2714 4406 5562 2714
Size: 1692 1156 3501
Fragments arranged by size:
3501 1692 1156
ApoI r'AATT_y
Cuts at: 1493 3658 3884 4036 6145 1493
Size: 2165 226 152 2109 1697
Fragments arranged by size:
2165 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2744 3471 2744
Size: 727 5622
AseI AT'TA_AT
Cuts at: 160 1032 4903 160
Size: 872 3871 1606
Fragments arranged by size:
3871 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
AvaI C'yCGr_G
Cuts at: 1870 1943 2309 2798 2951 3101 3245 3268 3346
Size: 73 366 489 153 150 144 23 78
Cuts at: 3346 3353 3674 1870
Size: 7 321 4545
Fragments arranged by size:
4545 489 366 321 153 150 144 78
73 23 7
BamHI G'GATC_C
Cuts at: 2334 3679 4046 2334
Size: 1345 367 4637
Fragments arranged by size:
4637 1345 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2822 3670 5125 618
Size: 325 217 627 1035 848 1455 1842
Fragments arranged by size:
1842 1455 1035 848 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2090 2310 2904 3042 3354 3614 3668 1391
Size: 699 220 594 138 312 260 54 4072
Fragments arranged by size:
4072 699 594 312 260 220 138 54
BbeI G_GCGC'C
Cuts at: 2826 2826
Size: 6349
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5378
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4540 4574 1903
Size: 34 2603 34 3678
Fragments arranged by size:
3678 2603 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4241 5451 4241
Size: 1210 5139
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6349
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2941 3146 3462
Size: 107 122 71 631 605 1269 205 316
Cuts at: 3462 4960 136
Size: 1498 1525
Fragments arranged by size:
1525 1498 1269 631 605 316 205 122
107 71
BglII A'GATC_T
Cuts at: 6344 6344
Size: 6349
BlpI GC'TnA_GC
Cuts at: 3766 3766
Size: 6349
Bme1580I G_kGCm'C
Cuts at: 1790 2718 3171 3354 4410 5566 1790
Size: 928 453 183 1056 1156 2573
Fragments arranged by size:
2573 1156 1056 928 453 183
BmgBI CAC'GTC
Cuts at: 1318 3211 1318
Size: 1893 4456
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4080 5038 442
Size: 416 53 83 399 24 2663 958 1753
Fragments arranged by size:
2663 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6349
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2492 2560 2492
Size: 68 6281
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5009 1239
Size: 3770 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2561 4474 5339 5637 2561
Size: 1913 865 298 3273
Fragments arranged by size:
3273 1913 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3090 5012 882
Size: 617 1591 1922 2219
Fragments arranged by size:
2219 1922 1591 617
BsaAI yAC'GTr
Cuts at: 493 1204 2395 2686 3646 4140 493
Size: 711 1191 291 960 494 2702
Fragments arranged by size:
2702 1191 960 711 494 291
BsaBI GATnn'nnATC
Cuts at: 6343 6343
Size: 6349
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2127 2540 2823 2933
Size: 53 83 186 936 594 413 283 110
Cuts at: 2933 3339 4156 4538 5173 275
Size: 406 817 382 635 1451
Fragments arranged by size:
1451 936 817 635 594 413 406 382
283 186 110 83 53
BsaWI w'CCGG_w
Cuts at: 2511 2529 4782 5180 5454 5601 6012 2511
Size: 18 2253 398 274 147 411 2848
Fragments arranged by size:
2848 2253 411 398 274 147 18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2708 2738 3266 3296 1178
Size: 30 165 30 1305 30 528 30 4231
Fragments arranged by size:
4231 1305 528 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 1186
Size: 889 5460
BseYI C'CCAG_C
Cuts at: 3407 3509 5552 6048 3407
Size: 102 2043 496 3708
Fragments arranged by size:
3708 2043 496 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2515 2948 1163
Size: 573 779 433 4564
Fragments arranged by size:
4564 779 573 433
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2242 3013 3141 3799 4560 5588
Size: 275 186 241 771 128 658 761 1028
Cuts at: 5588 1540
Size: 2301
Fragments arranged by size:
2301 1028 771 761 658 275 241 186
128
BsiHKAI G_wGCw'C
Cuts at: 2170 2718 2889 2904 4410 4495 5566 2170
Size: 548 171 15 1506 85 1071 2953
Fragments arranged by size:
2953 1506 1071 548 171 85 15
BsmI GAATG_Cn'
Cuts at: 2620 3860 3953 2620
Size: 1240 93 5016
Fragments arranged by size:
5016 1240 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2405 2531 2405
Size: 126 6223
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2090 2170 2310 2718 2889 2904 3042
Size: 399 300 80 140 408 171 15 138
Cuts at: 3042 3171 3354 3614 3668 4410 4495 5566 1391
Size: 129 183 260 54 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 408 399 300 260 183
171 140 138 129 85 80 54 15
BspEI T'CCGG_A
Cuts at: 6012 6012
Size: 6349
BspHI T'CATG_A
Cuts at: 4238 5968 4238
Size: 1730 4619
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2857 3704 844
Size: 2013 847 3489
Fragments arranged by size:
3489 2013 847
BsrBI CCG'CTC
Cuts at: 1394 1823 3253 3429 4236 1394
Size: 429 1430 176 807 3507
Fragments arranged by size:
3507 1430 807 429 176
BsrDI GCAATG_nn'
Cuts at: 65 1248 4844 5018 65
Size: 1183 3596 174 1396
Fragments arranged by size:
3596 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2078 2241 2499 3485 4993 5180 1700
Size: 110 268 163 258 986 1508 187 2869
Fragments arranged by size:
2869 1508 986 268 258 187 163 110
BsrGI T'GTAC_A
Cuts at: 96 3225 96
Size: 3129 3220
BssHII G'CGCG_C
Cuts at: 1925 2744 3199 3368 3471 1925
Size: 819 455 169 103 4803
Fragments arranged by size:
4803 819 455 169 103
BssSI C'ACGA_G
Cuts at: 2163 2888 4409 5421 2163
Size: 725 1521 1012 3091
Fragments arranged by size:
3091 1521 1012 725
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2502 3003 794
Size: 1708 501 4140
Fragments arranged by size:
4140 1708 501
BstBI TT'CG_AA
Cuts at: 2007 5153 2007
Size: 3146 3203
BstEII G'GTnAC_C
Cuts at: 1184 2965 1184
Size: 1781 4568
BstYI r'GATC_y
Cuts at: 1972 2334 3679 4046 4431 4448 5900 5986 5998
Size: 362 1345 367 385 17 1452 86 12
Cuts at: 5998 6344 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1345 385 367 362 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2198 2198
Size: 6349
BtgI C'CryG_G
Cuts at: 513 1065 2022 2136 2256 2814 2939 3394 3614
Size: 552 957 114 120 558 125 455 220
Cuts at: 3614 513
Size: 3248
Fragments arranged by size:
3248 957 558 552 455 220 125 120
114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3310 487
Size: 683 56 2084 3526
Fragments arranged by size:
3526 2084 683 56
BtsI GCAGTG_nn'
Cuts at: 3865 4659 4679 3865
Size: 794 20 5535
Fragments arranged by size:
5535 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6349
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2815 2850 529
Size: 35 2251 35 4028
Fragments arranged by size:
4028 2251 35 35
DraI TTT'AAA
Cuts at: 3653 3653
Size: 6349
DraIII CAC_nnn'GTG
Cuts at: 2224 2224
Size: 6349
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5356 809
Size: 507 4040 1802
Fragments arranged by size:
4040 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2229 2691 2734
Size: 54 1425 50 275 18 399 462 43
Cuts at: 2734 3010 3138 3213 3321 3483 3796 4685 6314
Size: 276 128 75 108 162 313 889 1629
Cuts at: 6314 8
Size: 43
Fragments arranged by size:
1629 1425 889 462 399 313 276 275
162 128 108 75 54 50 43 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3010 3138 3796 1537
Size: 275 1198 128 658 4090
Fragments arranged by size:
4090 1198 658 275 128
EarI CTCTTCn'nnn_
Cuts at: 1353 2510 4279 1353
Size: 1157 1769 3423
Fragments arranged by size:
3423 1769 1157
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2330 3221 3274 4936 5310 5456
Size: 1064 138 741 891 53 1662 374 146
Cuts at: 5456 387
Size: 1280
Fragments arranged by size:
1662 1280 1064 891 741 374 146 138
53
EcoICRI GAG'CTC
Cuts at: 2902 2902
Size: 6349
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2630 3149 3215 3292 4412 5009
Size: 393 443 555 519 66 77 1120 597
Cuts at: 5009 5796 6064 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1120 787 597 555 519 443 393
268 77 66
EcoNI CCTnn'n_nnAGG
Cuts at: 2057 2057
Size: 6349
EcoO109I rG'GnC_Cy
Cuts at: 3350 3504 3350
Size: 154 6195
EcoRI G'AATT_C
Cuts at: 3658 4036 3658
Size: 378 5971
FseI GG_CCGG'CC
Cuts at: 3489 3489
Size: 6349
FspI TGC'GCA
Cuts at: 4855 4855
Size: 6349
HaeII r_GCGC'y
Cuts at: 1678 2826 3559 5496 1678
Size: 1148 733 1937 2531
Fragments arranged by size:
2531 1937 1148 733
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2399 2431 4990 5022 5064
Size: 32 135 32 827 32 2559 32 42
Cuts at: 5064 5096 1373
Size: 32 2626
Fragments arranged by size:
2626 2559 827 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3693 3939 669
Size: 988 281 1755 246 3079
Fragments arranged by size:
3079 1755 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6349
HpaI GTT'AAC
Cuts at: 3939 3939
Size: 6349
KasI G'GCGC_C
Cuts at: 2822 2822
Size: 6349
KpnI G_GTAC'C
Cuts at: 3674 3674
Size: 6349
MfeI C'AATT_G
Cuts at: 3948 3948
Size: 6349
MluI A'CGCG_T
Cuts at: 6225 6225
Size: 6349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2217 2232 2724 5463 5647 1305
Size: 912 15 492 2739 184 2007
Fragments arranged by size:
2739 2007 912 492 184 15
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2231 3323 10
Size: 54 1425 343 399 1092 3036
Fragments arranged by size:
3036 1425 1092 399 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2666 2711 2924 4307 4666 4825 518
Size: 648 1500 45 213 1383 359 159 2042
Fragments arranged by size:
2042 1500 1383 648 359 213 159 45
MspA1I CmG'CkG
Cuts at: 1606 2043 2068 2138 2941 3319 3375 3396 3765
Size: 437 25 70 803 378 56 21 369
Cuts at: 3765 4442 5590 5835 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 803 677 437 378 369 245
70 56 25 21
NaeI GCC'GGC
Cuts at: 2080 3487 2080
Size: 1407 4942
NarI GG'CG_CC
Cuts at: 2823 2823
Size: 6349
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4840
Fragments arranged by size:
4840 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6349
NgoMIV G'CCGG_C
Cuts at: 2078 3485 2078
Size: 1407 4942
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6349
NotI GC'GGCC_GC
Cuts at: 3796 3796
Size: 6349
NspI r_CATG'y
Cuts at: 1132 1229 2638 2710 3707 5252 1132
Size: 97 1409 72 997 1545 2229
Fragments arranged by size:
2229 1545 1409 997 97 72
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6349
PciI A'CATG_T
Cuts at: 2706 5248 2706
Size: 2542 3807
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2653 1108
Size: 1545 4804
PfoI T'CCnGG_A
Cuts at: 2099 2121 2099
Size: 22 6327
PmeI GTTT'AAAC
Cuts at: 3653 3653
Size: 6349
PmlI CAC'GTG
Cuts at: 2686 2686
Size: 6349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4418 4450 5931 5963 4418
Size: 32 1481 32 4804
Fragments arranged by size:
4804 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3504 3504
Size: 6349
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6349
PsiI TTA'TAA
Cuts at: 3919 3919
Size: 6349
PspOMI G'GGCC_C
Cuts at: 3350 3350
Size: 6349
PstI C_TGCA'G
Cuts at: 830 3380 3701 830
Size: 2550 321 3478
Fragments arranged by size:
3478 2550 321
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6349
PvuII CAG'CTG
Cuts at: 3375 3375
Size: 6349
SacI G_AGCT'C
Cuts at: 2904 2904
Size: 6349
SacII CC_GC'GG
Cuts at: 2139 2942 3397 2139
Size: 803 455 5091
Fragments arranged by size:
5091 803 455
SalI G'TCGA_C
Cuts at: 1936 3691 1936
Size: 1755 4594
SanDI GG'GwC_CC
Cuts at: 3504 3504
Size: 6349
SapI GCTCTTCn'nnn_
Cuts at: 2510 2510
Size: 6349
SbfI CC_TGCA'GG
Cuts at: 3701 3701
Size: 6349
ScaI AGT'ACT
Cuts at: 4597 6309 4597
Size: 1712 4637
SfcI C'TryA_G
Cuts at: 826 1045 3068 3376 3697 4832 5513 5704 826
Size: 219 2023 308 321 1135 681 191 1471
Fragments arranged by size:
2023 1471 1135 681 321 308 219 191
SfoI GGC'GCC
Cuts at: 2824 2824
Size: 6349
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6349
SmaI CCC'GGG
Cuts at: 1872 2311 2800 2953 3247 3348 3676 1872
Size: 439 489 153 294 101 328 4545
Fragments arranged by size:
4545 489 439 328 294 153 101
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2576 4453 5354 5616 820
Size: 197 926 633 1877 901 262 1553
Fragments arranged by size:
1877 1553 926 901 633 262 197
SnaBI TAC'GTA
Cuts at: 493 3646 4140 493
Size: 3153 494 2702
Fragments arranged by size:
3153 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6349
SphI G_CATG'C
Cuts at: 1132 3707 1132
Size: 2575 3774
SspI AAT'ATT
Cuts at: 5 52 4273 5
Size: 47 4221 2081
Fragments arranged by size:
4221 2081 47
StuI AGG'CCT
Cuts at: 1517 3600 1517
Size: 2083 4266
StyI C'CwwG_G
Cuts at: 513 1065 2022 3114 3549 3788 513
Size: 552 957 1092 435 239 3074
Fragments arranged by size:
3074 1092 957 552 435 239
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2316 2495 3291 4376 4393 4546 4731
Size: 1255 736 179 796 1085 17 153 185
Cuts at: 4731 5176 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1085 796 736 445 185 179
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3225 4595 6307
Size: 274 80 33 51 1443 1248 1370 1712
Cuts at: 6307 96
Size: 138
Fragments arranged by size:
1712 1443 1370 1248 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2506 2538 2708 2740 3266 3298 6309 6341 2506
Size: 32 170 32 526 32 3011 32 2514
Fragments arranged by size:
3011 2514 526 170 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2552 1316
Size: 1236 5113
XbaI T'CTAG_A
Cuts at: 3685 3685
Size: 6349
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6349
XmaI C'CCGG_G
Cuts at: 1870 2309 2798 2951 3245 3346 3674 1870
Size: 439 489 153 294 101 328 4545
Fragments arranged by size:
4545 489 439 328 294 153 101
XmnI GAAnn'nnTTC
Cuts at: 4478 4478
Size: 6349
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4157 276
Size: 53 83 186 936 2623 2468
Fragments arranged by size:
2623 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BstXI BstZ17I EcoRV FalI FspAI NruI NsiI
PacI PspXI PsrI RsrII SexAI SfiI SrfI SwaI
XcmI