Restriction map for
pFN21ASDA1438
(Circular) (Six-base) MAPSORT of: pFN21ASDA1438.seq Check: 8709 from: 1 to: 7522
pFN21ASDA1438 7522 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5332 278
Size: 53 83 186 936 3796 2468
Fragments arranged by size:
3796 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4865 1937
Size: 2928 4594
Acc65I G'GTAC_C
Cuts at: 4843 4843
Size: 7522
AclI AA'CG_TT
Cuts at: 1563 5649 6022 1563
Size: 4086 373 3063
Fragments arranged by size:
4086 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2219 2225 2327 2729 3107 3119 4205 5585
Size: 587 6 102 402 378 12 1086 1380
Cuts at: 5585 6969 7237 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1380 1086 587 402 378 268
102 12 6
AfeI AGC'GCT
Cuts at: 3401 3401
Size: 7522
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7325
AflIII A'CryG_T
Cuts at: 1203 6421 7398 1203
Size: 5218 977 1327
Fragments arranged by size:
5218 1327 977
AgeI A'CCGG_T
Cuts at: 6353 6353
Size: 7522
AhdI GACnn_n'nnGTC
Cuts at: 6251 6251
Size: 7522
AleI CACnn'nnGTG
Cuts at: 1166 3743 1166
Size: 2577 4945
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4508 1184
Size: 3324 4198
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2098 2130 3749 3781 2098
Size: 32 1619 32 5839
Fragments arranged by size:
5839 1619 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3097 3601 3661 3793 4115 4564 5265 6837 3097
Size: 504 60 132 322 449 701 1572 3782
Fragments arranged by size:
3782 1572 701 504 449 322 132 60
ApaI G_GGCC'C
Cuts at: 2997 3530 3800 3876 3962 2997
Size: 533 270 76 86 6557
Fragments arranged by size:
6557 533 270 86 76
ApaLI G'TGCA_C
Cuts at: 5579 6735 5579
Size: 1156 6366
ApoI r'AATT_y
Cuts at: 1493 4302 4831 5057 5209 7318 1493
Size: 2809 529 226 152 2109 1697
Fragments arranged by size:
2809 2109 1697 529 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6076 160
Size: 872 5044 1606
Fragments arranged by size:
5044 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7522
AvaI C'yCGr_G
Cuts at: 1870 1943 2396 2451 2980 3121 3437 3531 3868
Size: 73 453 55 529 141 316 94 337
Cuts at: 3868 3917 4847 1870
Size: 49 930 4545
Fragments arranged by size:
4545 930 529 453 337 316 141 94
73 55 49
BamHI G'GATC_C
Cuts at: 4852 5219 4852
Size: 367 7155
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2776 2984 3125 3737 4843
Size: 325 217 627 989 208 141 612 1106
Cuts at: 4843 6298 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1106 989 627 612 325 217
208 141
BanII G_rGCy'C
Cuts at: 1391 2997 3049 3193 3368 3530 3557 3706 3800
Size: 1606 52 144 175 162 27 149 94
Cuts at: 3800 3876 3918 3962 4079 4413 4530 4638 4841
Size: 76 42 44 117 334 117 108 203
Cuts at: 4841 1391
Size: 4072
Fragments arranged by size:
4072 1606 334 203 175 162 149 144
117 117 108 94 76 52 44 42
27
BbeI G_GCGC'C
Cuts at: 2988 2988
Size: 7522
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4367 928
Size: 971 2468 4083
Fragments arranged by size:
4083 2468 971
BbvCI CC'TCA_GC
Cuts at: 3309 4736 3309
Size: 1427 6095
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2387 2421 5713 5747 1903
Size: 34 450 34 3292 34 3678
Fragments arranged by size:
3678 3292 450 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5414 6624 5414
Size: 1210 6312
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7522
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3001 3053 3197
Size: 107 122 71 631 605 1329 52 144
Cuts at: 3197 3287 3329 6133 136
Size: 90 42 2804 1525
Fragments arranged by size:
2804 1525 1329 631 605 144 122 107
90 71 52 42
BglII A'GATC_T
Cuts at: 7517 7517
Size: 7522
BlpI GC'TnA_GC
Cuts at: 2172 3266 3410 3779 4407 4565 4721 4939 2172
Size: 1094 144 369 628 158 156 218 4755
Fragments arranged by size:
4755 1094 628 369 218 158 156 144
Bme1580I G_kGCm'C
Cuts at: 1790 2779 2997 3128 3530 3800 3876 3962 5583
Size: 989 218 131 402 270 76 86 1621
Cuts at: 5583 6739 1790
Size: 1156 2573
Fragments arranged by size:
2573 1621 1156 989 402 270 218 131
86 76
BmgBI CAC'GTC
Cuts at: 1318 3011 1318
Size: 1693 5829
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2936 3931 4810
Size: 416 53 83 399 24 1519 995 879
Cuts at: 4810 5253 6211 442
Size: 443 958 1753
Fragments arranged by size:
1753 1519 995 958 879 443 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7522
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2168 2465 2816 2959 3288 3707 3751 4092
Size: 929 297 351 143 329 419 44 341
Cuts at: 4092 4160 4419 4484 4496 4715 6182 1239
Size: 68 259 65 12 219 1467 2579
Fragments arranged by size:
2579 1467 929 419 351 341 329 297
259 219 143 68 65 44 12
Bpu10I CC'TnA_GC
Cuts at: 3309 3551 3912 4632 4736 3309
Size: 242 361 720 104 6095
Fragments arranged by size:
6095 720 361 242 104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2714 3659 4237 5647 6512 6810 2714
Size: 945 578 1410 865 298 3426
Fragments arranged by size:
3426 1410 945 865 578 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2156 6185 882
Size: 617 657 4029 2219
Fragments arranged by size:
4029 2219 657 617
BsaAI yAC'GTr
Cuts at: 493 1204 4819 5313 493
Size: 711 3615 494 2702
Fragments arranged by size:
3615 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7516 7516
Size: 7522
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2985 4459 5329 5711
Size: 53 83 186 936 1452 1474 870 382
Cuts at: 5711 6346 275
Size: 635 1451
Fragments arranged by size:
1474 1452 1451 936 870 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5955 6353 6627 6774 7185 5955
Size: 398 274 147 411 6292
Fragments arranged by size:
6292 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3086 3116 3749 3779 4434
Size: 30 165 30 1683 30 633 30 655
Cuts at: 4434 4464 4688 4718 1178
Size: 30 224 30 3982
Fragments arranged by size:
3982 1683 655 633 224 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2553 3536 4422 1186
Size: 1367 983 886 4286
Fragments arranged by size:
4286 1367 983 886
BseYI C'CCAG_C
Cuts at: 2591 3253 3705 3818 3832 3962 4080 4095 4637
Size: 662 452 113 14 130 118 15 542
Cuts at: 4637 4751 6725 7221 2591
Size: 114 1974 496 2892
Fragments arranged by size:
2892 1974 662 542 496 452 130 118
114 113 15 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3206 4781 1163
Size: 573 1470 1575 3904
Fragments arranged by size:
3904 1575 1470 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2123 4972 5733 6761 1540
Size: 275 186 122 2849 761 1028 2301
Fragments arranged by size:
2849 2301 1028 761 275 186 122
BsiHKAI G_wGCw'C
Cuts at: 3272 4146 4413 5583 5668 6739 3272
Size: 874 267 1170 85 1071 4055
Fragments arranged by size:
4055 1170 1071 874 267 85
BsmI GAATG_Cn'
Cuts at: 4051 5033 5126 4051
Size: 982 93 6447
Fragments arranged by size:
6447 982 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2779 2997 3049 3128 3193 3272 3368
Size: 399 989 218 52 79 65 79 96
Cuts at: 3368 3530 3557 3706 3800 3876 3918 3962 4079
Size: 162 27 149 94 76 42 44 117
Cuts at: 4079 4146 4413 4530 4638 4841 5583 5668 6739
Size: 67 267 117 108 203 742 85 1071
Cuts at: 6739 1391
Size: 2174
Fragments arranged by size:
2174 1071 989 742 399 267 218 203
162 149 117 117 108 96 94 85
79 79 76 67 65 52 44 42
27
BspEI T'CCGG_A
Cuts at: 7185 7185
Size: 7522
BspHI T'CATG_A
Cuts at: 2053 5411 7141 2053
Size: 3358 1730 2434
Fragments arranged by size:
3358 2434 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4877 844
Size: 4033 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2077 3670 3803 4296 5409 1394
Size: 429 254 1593 133 493 1113 3507
Fragments arranged by size:
3507 1593 1113 493 429 254 133
BsrDI GCAATG_nn'
Cuts at: 65 1248 6017 6191 65
Size: 1183 4769 174 1396
Fragments arranged by size:
4769 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3052 3922 6166 6353 1700
Size: 110 1242 870 2244 187 2869
Fragments arranged by size:
2869 2244 1242 870 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7522
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7522
BssSI C'ACGA_G
Cuts at: 3512 5582 6594 3512
Size: 2070 1012 4440
Fragments arranged by size:
4440 2070 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4150 794
Size: 3356 4166
BstBI TT'CG_AA
Cuts at: 2007 6326 2007
Size: 4319 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7522
BstXI CCAn_nnnn'nTGG
Cuts at: 2269 2323 4654 2269
Size: 54 2331 5137
Fragments arranged by size:
5137 2331 54
BstYI r'GATC_y
Cuts at: 1972 2805 2841 3507 4852 5219 5604 5621 7073
Size: 833 36 666 1345 367 385 17 1452
Cuts at: 7073 7159 7171 7517 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1345 833 666 385 367 346
86 36 17 12
Bsu36I CC'TnA_GG
Cuts at: 3954 3954
Size: 7522
BtgI C'CryG_G
Cuts at: 513 1065 2262 2784 3324 3882 4602 4647 513
Size: 552 1197 522 540 558 720 45 3388
Fragments arranged by size:
3388 1197 720 558 552 540 522 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2397 3435 487
Size: 683 56 1171 1038 4574
Fragments arranged by size:
4574 1171 1038 683 56
BtsI GCAGTG_nn'
Cuts at: 2465 2619 3110 3343 3404 3500 4148 4572 5038
Size: 154 491 233 61 96 648 424 466
Cuts at: 5038 5832 5852 2465
Size: 794 20 4135
Fragments arranged by size:
4135 794 648 491 466 424 233 154
96 61 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7522
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2482 2517 529
Size: 35 1918 35 5534
Fragments arranged by size:
5534 1918 35 35
DraI TTT'AAA
Cuts at: 4826 4826
Size: 7522
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6529 809
Size: 507 5213 1802
Fragments arranged by size:
5213 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2120 3054 3321
Size: 54 1425 50 275 18 290 934 267
Cuts at: 3321 3330 4669 4780 4969 5858 7487 8
Size: 9 1339 111 189 889 1629 43
Fragments arranged by size:
1629 1425 1339 934 889 290 275 267
189 111 54 50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2120 4969 1537
Size: 275 308 2849 4090
Fragments arranged by size:
4090 2849 308 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2154 2201 2365 2831 4763 5452 1353
Size: 801 47 164 466 1932 689 3423
Fragments arranged by size:
3423 1932 801 689 466 164 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3584 6109 6483 6629 387
Size: 1064 138 1995 2525 374 146 1280
Fragments arranged by size:
2525 1995 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4411 4411
Size: 7522
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2168 2219 2225 2327 2465 2729 2816
Size: 393 536 51 6 102 138 264 87
Cuts at: 2816 2959 3107 3119 3288 3707 3751 4092 4160
Size: 143 148 12 169 419 44 341 68
Cuts at: 4160 4205 4419 4484 4496 4715 5585 6182 6969
Size: 45 214 65 12 219 870 597 787
Cuts at: 6969 7237 1239
Size: 268 1524
Fragments arranged by size:
1524 870 787 597 536 419 393 341
268 264 219 214 169 148 143 138
102 87 68 65 51 45 44 12
12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3183 3224 3515 3971 4434 3183
Size: 41 291 456 463 6271
Fragments arranged by size:
6271 463 456 291 41
EcoO109I rG'GnC_Cy
Cuts at: 2917 2934 2994 3199 3526 3527 3540 3797 3872
Size: 17 60 205 327 1 13 257 75
Cuts at: 3872 3873 3950 3958 3959 4014 4167 4374 2917
Size: 1 77 8 1 55 153 207 6065
Fragments arranged by size:
6065 327 257 207 205 153 77 75
60 55 17 13 8 1 1 1
EcoRI G'AATT_C
Cuts at: 4831 5209 4831
Size: 378 7144
FspI TGC'GCA
Cuts at: 6028 6028
Size: 7522
HaeII r_GCGC'y
Cuts at: 1678 2988 3151 3403 3632 4115 6669 1678
Size: 1310 163 252 229 483 2554 2531
Fragments arranged by size:
2554 2531 1310 483 252 229 163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2375 2407 3413 3445 3454
Size: 32 135 32 803 32 1006 32 9
Cuts at: 3454 3486 4313 4337 4345 4369 4688 4720 6163
Size: 32 827 24 8 24 319 32 1443
Cuts at: 6163 6195 6237 6269 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1443 1006 827 803 319 135 42
32 32 32 32 32 32 32 32
24 24 9 8
HincII GTy'rAC
Cuts at: 669 1657 1938 4866 5112 669
Size: 988 281 2928 246 3079
Fragments arranged by size:
3079 2928 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7522
HpaI GTT'AAC
Cuts at: 5112 5112
Size: 7522
KasI G'GCGC_C
Cuts at: 2984 2984
Size: 7522
KpnI G_GTAC'C
Cuts at: 4847 4847
Size: 7522
MfeI C'AATT_G
Cuts at: 5121 5121
Size: 7522
MluI A'CGCG_T
Cuts at: 7398 7398
Size: 7522
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6636 6820 1305
Size: 5331 184 2007
Fragments arranged by size:
5331 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3323 3332 4671 4782 10
Size: 54 1425 343 1491 9 1339 111 2750
Fragments arranged by size:
2750 1491 1425 1339 343 111 54 9
MslI CAynn'nnrTG
Cuts at: 518 1166 2321 3074 3743 5480 5839 5998 518
Size: 648 1155 753 669 1737 359 159 2042
Fragments arranged by size:
2042 1737 1155 753 669 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2197 2836 2931 3257 3308 3481 3661 3685
Size: 591 639 95 326 51 173 180 24
Cuts at: 3685 3771 3778 4795 4938 5615 6763 7008 1606
Size: 86 7 1017 143 677 1148 245 2120
Fragments arranged by size:
2120 1148 1017 677 639 591 326 245
180 173 143 95 86 51 24 7
NaeI GCC'GGC
Cuts at: 3054 3054
Size: 7522
NarI GG'CG_CC
Cuts at: 2985 2985
Size: 7522
NcoI C'CATG_G
Cuts at: 513 1065 2262 2784 4602 4647 513
Size: 552 1197 522 1818 45 3388
Fragments arranged by size:
3388 1818 1197 552 522 45
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7522
NgoMIV G'CCGG_C
Cuts at: 3052 3052
Size: 7522
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7522
NotI GC'GGCC_GC
Cuts at: 4969 4969
Size: 7522
NspI r_CATG'y
Cuts at: 1132 1229 3643 4880 6425 1132
Size: 97 2414 1237 1545 2229
Fragments arranged by size:
2414 2229 1545 1237 97
PasI CC'CwG_GG
Cuts at: 1348 2422 2920 2990 3557 3793 1348
Size: 1074 498 70 567 236 5077
Fragments arranged by size:
5077 1074 567 498 236 70
PciI A'CATG_T
Cuts at: 6421 6421
Size: 7522
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4264 1108
Size: 3156 4366
PfoI T'CCnGG_A
Cuts at: 4473 4581 4473
Size: 108 7414
PmeI GTTT'AAAC
Cuts at: 4826 4826
Size: 7522
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2454 2486 3008 3040 3749 3781 5591 5623 7104
Size: 32 522 32 709 32 1810 32 1481
Cuts at: 7104 7136 2454
Size: 32 2840
Fragments arranged by size:
2840 1810 1481 709 522 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2934 3540 4014 4167 4374 2934
Size: 606 474 153 207 6082
Fragments arranged by size:
6082 606 474 207 153
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7522
PsiI TTA'TAA
Cuts at: 5092 5092
Size: 7522
PspOMI G'GGCC_C
Cuts at: 2993 3526 3796 3872 3958 2993
Size: 533 270 76 86 6557
Fragments arranged by size:
6557 533 270 86 76
PstI C_TGCA'G
Cuts at: 830 2814 2841 3492 3507 3606 3684 4155 4874
Size: 1984 27 651 15 99 78 471 719
Cuts at: 4874 830
Size: 3478
Fragments arranged by size:
3478 1984 719 651 471 99 78 27
15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7522
PvuII CAG'CTG
Cuts at: 2197 2836 3308 3481 3661 3685 3771 3778 4795
Size: 639 472 173 180 24 86 7 1017
Cuts at: 4795 2197
Size: 4924
Fragments arranged by size:
4924 1017 639 472 180 173 86 24
7
SacI G_AGCT'C
Cuts at: 4413 4413
Size: 7522
SalI G'TCGA_C
Cuts at: 1936 4864 1936
Size: 2928 4594
SanDI GG'GwC_CC
Cuts at: 2934 4014 4167 4374 2934
Size: 1080 153 207 6082
Fragments arranged by size:
6082 1080 207 153
SapI GCTCTTCn'nnn_
Cuts at: 2365 2831 2365
Size: 466 7056
SbfI CC_TGCA'GG
Cuts at: 3492 4874 3492
Size: 1382 6140
ScaI AGT'ACT
Cuts at: 5770 7482 5770
Size: 1712 5810
SfcI C'TryA_G
Cuts at: 826 1045 2810 2837 3488 3503 3602 3680 4151
Size: 219 1765 27 651 15 99 78 471
Cuts at: 4151 4870 6005 6686 6877 826
Size: 719 1135 681 191 1471
Fragments arranged by size:
1765 1471 1135 719 681 651 471 219
191 99 78 27 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 3329 3329
Size: 7522
SfoI GGC'GCC
Cuts at: 2986 2986
Size: 7522
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7522
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7522
SmaI CCC'GGG
Cuts at: 1872 3439 3533 3870 4849 1872
Size: 1567 94 337 979 4545
Fragments arranged by size:
4545 1567 979 337 94
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2729 3674 4252 5626 6527 6789
Size: 197 926 786 945 578 1374 901 262
Cuts at: 6789 820
Size: 1553
Fragments arranged by size:
1553 1374 945 926 901 786 578 262
197
SnaBI TAC'GTA
Cuts at: 493 4819 5313 493
Size: 4326 494 2702
Fragments arranged by size:
4326 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7522
SphI G_CATG'C
Cuts at: 1132 3643 4880 1132
Size: 2511 1237 3774
Fragments arranged by size:
3774 2511 1237
SspI AAT'ATT
Cuts at: 5 52 5446 5
Size: 47 5394 2081
Fragments arranged by size:
5394 2081 47
StuI AGG'CCT
Cuts at: 1517 2254 3297 3494 1517
Size: 737 1043 197 5545
Fragments arranged by size:
5545 1043 737 197
StyI C'CwwG_G
Cuts at: 513 1065 2262 2700 2784 3195 4602 4647 4961
Size: 552 1197 438 84 411 1407 45 314
Cuts at: 4961 513
Size: 3074
Fragments arranged by size:
3074 1407 1197 552 438 411 314 84
45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2510 3822 4161 4615 5549 5566 5719
Size: 1255 930 1312 339 454 934 17 153
Cuts at: 5719 5904 6349 325
Size: 185 445 1498
Fragments arranged by size:
1498 1312 1255 934 930 454 445 339
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2742 5768 7480
Size: 274 80 33 51 1443 765 3026 1712
Cuts at: 7480 96
Size: 138
Fragments arranged by size:
3026 1712 1443 765 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3492 3524 4434 4466 4790 4822 7482 7514 3492
Size: 32 910 32 324 32 2660 32 3500
Fragments arranged by size:
3500 2660 910 324 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7522
XbaI T'CTAG_A
Cuts at: 4858 4858
Size: 7522
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2269 3340 4645 2269
Size: 1071 1305 5146
Fragments arranged by size:
5146 1305 1071
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7522
XmaI C'CCGG_G
Cuts at: 1870 3437 3531 3868 4847 1870
Size: 1567 94 337 979 4545
Fragments arranged by size:
4545 1567 979 337 94
XmnI GAAnn'nnTTC
Cuts at: 3761 5651 3761
Size: 1890 5632
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5330 276
Size: 53 83 186 936 3796 2468
Fragments arranged by size:
3796 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI
PacI PmlI PspXI PsrI RsrII SacII SexAI SrfI
SwaI