Restriction map for
pFN21ASDA1437
(Circular) (Six-base) MAPSORT of: pFN21ASDA1437.seq Check: 5382 from: 1 to: 7159
pFN21ASDA1437 7159 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3735 3942 3956 3735
Size: 207 14 6938
Fragments arranged by size:
6938 207 14
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3126 4969 278
Size: 53 83 186 936 1590 1843 2468
Fragments arranged by size:
2468 1843 1590 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2871 3035 4502 1937
Size: 934 164 1467 4594
Fragments arranged by size:
4594 1467 934 164
Acc65I G'GTAC_C
Cuts at: 4480 4480
Size: 7159
AclI AA'CG_TT
Cuts at: 1563 5286 5659 1563
Size: 3723 373 3063
Fragments arranged by size:
3723 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2102 2525 2566 3587 3838 5222 6606 6874
Size: 470 423 41 1021 251 1384 1384 268
Cuts at: 6874 1632
Size: 1917
Fragments arranged by size:
1917 1384 1384 1021 470 423 268 251
41
AfeI AGC'GCT
Cuts at: 3188 3188
Size: 7159
AflII C'TTAA_G
Cuts at: 820 1017 3953 820
Size: 197 2936 4026
Fragments arranged by size:
4026 2936 197
AflIII A'CryG_T
Cuts at: 1203 6058 7035 1203
Size: 4855 977 1327
Fragments arranged by size:
4855 1327 977
AgeI A'CCGG_T
Cuts at: 5990 5990
Size: 7159
AhdI GACnn_n'nnGTC
Cuts at: 2611 5888 2611
Size: 3277 3882
AleI CACnn'nnGTG
Cuts at: 1166 3043 3713 1166
Size: 1877 670 4612
Fragments arranged by size:
4612 1877 670
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2400 4262 1184
Size: 1216 1862 4081
Fragments arranged by size:
4081 1862 1216
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2011 2043 4376 4408 2011
Size: 32 2333 32 4762
Fragments arranged by size:
4762 2333 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2246 3736 3934 4258 4902 6474 2246
Size: 1490 198 324 644 1572 2931
Fragments arranged by size:
2931 1572 1490 644 324 198
ApaI G_GGCC'C
Cuts at: 2303 2539 4228 2303
Size: 236 1689 5234
Fragments arranged by size:
5234 1689 236
ApaLI G'TGCA_C
Cuts at: 2879 3722 5216 6372 2879
Size: 843 1494 1156 3666
Fragments arranged by size:
3666 1494 1156 843
ApoI r'AATT_y
Cuts at: 1493 2456 4468 4694 4846 6955 1493
Size: 963 2012 226 152 2109 1697
Fragments arranged by size:
2109 2012 1697 963 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5713 160
Size: 872 4681 1606
Fragments arranged by size:
4681 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7159
AvaI C'yCGr_G
Cuts at: 1870 1943 4409 4484 1870
Size: 73 2466 75 4545
Fragments arranged by size:
4545 2466 75 73
BamHI G'GATC_C
Cuts at: 2076 4489 4856 2076
Size: 2413 367 4379
Fragments arranged by size:
4379 2413 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2331 3755 4480 5935 618
Size: 325 217 627 544 1424 725 1455 1842
Fragments arranged by size:
1842 1455 1424 725 627 544 325 217
BanII G_rGCy'C
Cuts at: 1391 2043 2259 2303 2539 3651 4203 4228 4478
Size: 652 216 44 236 1112 552 25 250
Cuts at: 4478 1391
Size: 4072
Fragments arranged by size:
4072 1112 652 552 250 236 216 44
25
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2134 3190 3575 928
Size: 971 235 1056 385 4512
Fragments arranged by size:
4512 1056 971 385 235
BbvCI CC'TCA_GC
Cuts at: 4097 4097
Size: 7159
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4024 4058 5350 5384 1903
Size: 34 2087 34 1292 34 3678
Fragments arranged by size:
3678 2087 1292 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5051 6261 5051
Size: 1210 5949
BclI T'GATC_A
Cuts at: 1548 2838 1548
Size: 1290 5869
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2307 2931 4169
Size: 107 122 71 631 605 635 624 1238
Cuts at: 4169 5770 136
Size: 1601 1525
Fragments arranged by size:
1601 1525 1238 635 631 624 605 122
107 71
BglII A'GATC_T
Cuts at: 7154 7154
Size: 7159
BlpI GC'TnA_GC
Cuts at: 4576 4576
Size: 7159
Bme1580I G_kGCm'C
Cuts at: 1790 2303 2539 2652 2883 2956 3726 3758 4228
Size: 513 236 113 231 73 770 32 470
Cuts at: 4228 4352 5220 6376 1790
Size: 124 868 1156 2573
Fragments arranged by size:
2573 1156 868 770 513 470 236 231
124 113 73 32
BmgBI CAC'GTC
Cuts at: 1318 2635 2899 3397 1318
Size: 1317 264 498 5080
Fragments arranged by size:
5080 1317 498 264
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4890 5848 442
Size: 416 53 83 399 24 3473 958 1753
Fragments arranged by size:
3473 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7159
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2501 3326 3386 3401 3596 3830 3851 4034
Size: 1262 825 60 15 195 234 21 183
Cuts at: 4034 4136 4249 4340 5819 1239
Size: 102 113 91 1479 2579
Fragments arranged by size:
2579 1479 1262 825 234 195 183 113
102 91 60 21 15
Bpu10I CC'TnA_GC
Cuts at: 3602 3686 4097 3602
Size: 84 411 6664
Fragments arranged by size:
6664 411 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3050 3359 3419 3644 3659 3767 3869 3884 4343
Size: 309 60 225 15 108 102 15 459
Cuts at: 4343 5284 6149 6447 3050
Size: 941 865 298 3762
Fragments arranged by size:
3762 941 865 459 309 298 225 108
102 60 15 15
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5822 882
Size: 617 4323 2219
Fragments arranged by size:
4323 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2347 2878 4456 4950 493
Size: 711 1143 531 1578 494 2702
Fragments arranged by size:
2702 1578 1143 711 531 494
BsaBI GATnn'nnATC
Cuts at: 7153 7153
Size: 7159
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2987 3123 4966 5348
Size: 53 83 186 936 1454 136 1843 382
Cuts at: 5348 5983 275
Size: 635 1451
Fragments arranged by size:
1843 1454 1451 936 635 382 186 136
83 53
BsaWI w'CCGG_w
Cuts at: 2029 2073 2772 4176 4195 5592 5990 6264 6411
Size: 44 699 1404 19 1397 398 274 147
Cuts at: 6411 6822 2029
Size: 411 2366
Fragments arranged by size:
2366 1404 1397 699 411 398 274 147
44 19
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2255 2285 4177 4207 4376
Size: 30 165 30 852 30 1892 30 169
Cuts at: 4376 4406 4412 4442 1178
Size: 30 6 30 3895
Fragments arranged by size:
3895 1892 852 169 165 30 30 30
30 30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2573 2801 3110 3593 3926 4397 1186
Size: 1387 228 309 483 333 471 3948
Fragments arranged by size:
3948 1387 483 471 333 309 228
BseYI C'CCAG_C
Cuts at: 2235 3410 3420 4059 4339 6362 6858 2235
Size: 1175 10 639 280 2023 496 2536
Fragments arranged by size:
2536 2023 1175 639 496 280 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2360 2399 2890 3134 3296 3500 3569
Size: 573 624 39 491 244 162 204 69
Cuts at: 3569 3920 4214 1163
Size: 351 294 4108
Fragments arranged by size:
4108 624 573 491 351 294 244 204
162 69 39
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2622 4609 5370 6398 1540
Size: 275 186 621 1987 761 1028 2301
Fragments arranged by size:
2301 1987 1028 761 621 275 186
BsiHKAI G_wGCw'C
Cuts at: 2043 2487 2883 3651 3726 4203 5220 5305 6376
Size: 444 396 768 75 477 1017 85 1071
Cuts at: 6376 2043
Size: 2826
Fragments arranged by size:
2826 1071 1017 768 477 444 396 85
75
BsmI GAATG_Cn'
Cuts at: 4670 4763 4670
Size: 93 7066
BsmBI CGTCTCn'nnnn_
Cuts at: 2699 4178 2699
Size: 1479 5680
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2259 2303 2487 2539 2652 2883
Size: 399 253 216 44 184 52 113 231
Cuts at: 2883 2956 3651 3726 3758 4203 4228 4352 4478
Size: 73 695 75 32 445 25 124 126
Cuts at: 4478 5220 5305 6376 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 695 445 399 253 231
216 184 126 124 113 85 75 73
52 44 32 25
BspEI T'CCGG_A
Cuts at: 2772 4195 6822 2772
Size: 1423 2627 3109
Fragments arranged by size:
3109 2627 1423
BspHI T'CATG_A
Cuts at: 5048 6778 5048
Size: 1730 5429
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2149 3513 3735 3876 3942 3956 4514 844
Size: 1305 1364 222 141 66 14 558 3489
Fragments arranged by size:
3489 1364 1305 558 222 141 66 14
BsrBI CCG'CTC
Cuts at: 1394 1823 2700 3175 4423 5046 1394
Size: 429 877 475 1248 623 3507
Fragments arranged by size:
3507 1248 877 623 475 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2224 3414 5654 5828 65
Size: 1183 976 1190 2240 174 1396
Fragments arranged by size:
2240 1396 1190 1183 976 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2328 2575 4314 5803 5990 1700
Size: 110 518 247 1739 1489 187 2869
Fragments arranged by size:
2869 1739 1489 518 247 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7159
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7159
BssSI C'ACGA_G
Cuts at: 2530 5219 6231 2530
Size: 2689 1012 3458
Fragments arranged by size:
3458 2689 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4258 794
Size: 3464 3695
BstBI TT'CG_AA
Cuts at: 2007 5963 2007
Size: 3956 3203
BstEII G'GTnAC_C
Cuts at: 1184 2161 2200 1184
Size: 977 39 6143
Fragments arranged by size:
6143 977 39
BstYI r'GATC_y
Cuts at: 1972 2076 2979 3543 3555 4050 4489 4856 5241
Size: 104 903 564 12 495 439 367 385
Cuts at: 5241 5258 6710 6796 6808 7154 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 903 564 495 439 385 367
346 104 86 17 12 12
BstZ17I GTA'TAC
Cuts at: 3036 3036
Size: 7159
BtgI C'CryG_G
Cuts at: 513 1065 2087 2601 2910 513
Size: 552 1022 514 309 4762
Fragments arranged by size:
4762 1022 552 514 309
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3958 487
Size: 683 56 2732 3688
Fragments arranged by size:
3688 2732 683 56
BtsI GCAGTG_nn'
Cuts at: 4256 4352 4400 4675 5469 5489 4256
Size: 96 48 275 794 20 5926
Fragments arranged by size:
5926 794 275 96 48 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7159
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7124
DraI TTT'AAA
Cuts at: 4463 4463
Size: 7159
DraIII CAC_nnn'GTG
Cuts at: 2491 2491
Size: 7159
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6166 809
Size: 507 4850 1802
Fragments arranged by size:
4850 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2964 3462 4170
Size: 54 1425 50 275 18 1134 498 708
Cuts at: 4170 4606 5495 7124 8
Size: 436 889 1629 43
Fragments arranged by size:
1629 1425 1134 889 708 498 436 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4606 1537
Size: 275 2794 4090
Fragments arranged by size:
4090 2794 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2978 4070 4208 5089 1353
Size: 1625 1092 138 881 3423
Fragments arranged by size:
3423 1625 1092 881 138
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5746 6120 6266 387
Size: 1064 138 4157 374 146 1280
Fragments arranged by size:
4157 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2041 3649 4201 2041
Size: 1608 552 4999
Fragments arranged by size:
4999 1608 552
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2501 2525 2566 3326 3386 3401
Size: 393 470 399 24 41 760 60 15
Cuts at: 3401 3587 3596 3830 3838 3851 4034 4136 4249
Size: 186 9 234 8 13 183 102 113
Cuts at: 4249 4340 5222 5819 6606 6874 1239
Size: 91 882 597 787 268 1524
Fragments arranged by size:
1524 882 787 760 597 470 399 393
268 234 186 183 113 102 91 60
41 24 15 13 9 8
EcoNI CCTnn'n_nnAGG
Cuts at: 3206 3206
Size: 7159
EcoO109I rG'GnC_Cy
Cuts at: 2300 2535 2536 3370 4225 4387 2300
Size: 235 1 834 855 162 5072
Fragments arranged by size:
5072 855 834 235 162 1
EcoRI G'AATT_C
Cuts at: 4468 4846 4468
Size: 378 6781
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2596 3151 2596
Size: 555 6604
FspI TGC'GCA
Cuts at: 5665 5665
Size: 7159
HaeII r_GCGC'y
Cuts at: 1678 2750 3061 3190 3479 3515 4021 6306 1678
Size: 1072 311 129 289 36 506 2285 2531
Fragments arranged by size:
2531 2285 1072 506 311 289 129 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2099 2131 2597 2629 4177
Size: 32 135 32 527 32 466 32 1548
Cuts at: 4177 4209 5800 5832 5874 5906 1373
Size: 32 1591 32 42 32 2626
Fragments arranged by size:
2626 1591 1548 527 466 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4503 4749 669
Size: 988 281 2565 246 3079
Fragments arranged by size:
3079 2565 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7159
HpaI GTT'AAC
Cuts at: 4749 4749
Size: 7159
KpnI G_GTAC'C
Cuts at: 4484 4484
Size: 7159
MfeI C'AATT_G
Cuts at: 4758 4758
Size: 7159
MluI A'CGCG_T
Cuts at: 7035 7035
Size: 7159
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2523 3186 6273 6457 1305
Size: 1218 663 3087 184 2007
Fragments arranged by size:
3087 2007 1218 663 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2966 10
Size: 54 1425 343 1134 4203
Fragments arranged by size:
4203 1425 1134 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2129 2834 3029 3043 3713 5117 5476
Size: 648 963 705 195 14 670 1404 359
Cuts at: 5476 5635 518
Size: 159 2042
Fragments arranged by size:
2042 1404 963 705 670 648 359 195
159 14
MspA1I CmG'CkG
Cuts at: 1606 2662 2949 3235 3277 3461 3550 3822 4368
Size: 1056 287 286 42 184 89 272 546
Cuts at: 4368 4575 5252 6400 6645 1606
Size: 207 677 1148 245 2120
Fragments arranged by size:
2120 1148 1056 677 546 287 286 272
245 207 184 89 42
NaeI GCC'GGC
Cuts at: 2577 2577
Size: 7159
NcoI C'CATG_G
Cuts at: 513 1065 2601 513
Size: 552 1536 5071
Fragments arranged by size:
5071 1536 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7159
NgoMIV G'CCGG_C
Cuts at: 2575 2575
Size: 7159
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7159
NotI GC'GGCC_GC
Cuts at: 4606 4606
Size: 7159
NspI r_CATG'y
Cuts at: 1132 1229 2818 3034 4517 6062 1132
Size: 97 1589 216 1483 1545 2229
Fragments arranged by size:
2229 1589 1545 1483 216 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7159
PciI A'CATG_T
Cuts at: 6058 6058
Size: 7159
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2962 1108
Size: 1854 5305
PmeI GTTT'AAAC
Cuts at: 4463 4463
Size: 7159
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4376 4408 5228 5260 6741 6773 4376
Size: 32 820 32 1481 32 4762
Fragments arranged by size:
4762 1481 820 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3370 4387 3370
Size: 1017 6142
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7159
PsiI TTA'TAA
Cuts at: 4729 4729
Size: 7159
PspOMI G'GGCC_C
Cuts at: 2299 2535 4224 2299
Size: 236 1689 5234
Fragments arranged by size:
5234 1689 236
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3605 3637 3605
Size: 32 7127
PstI C_TGCA'G
Cuts at: 830 2160 2441 2661 3732 3873 4146 4254 4511
Size: 1330 281 220 1071 141 273 108 257
Cuts at: 4511 830
Size: 3478
Fragments arranged by size:
3478 1330 1071 281 273 257 220 141
108
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7159
PvuII CAG'CTG
Cuts at: 3235 3235
Size: 7159
SacI G_AGCT'C
Cuts at: 2043 3651 4203 2043
Size: 1608 552 4999
Fragments arranged by size:
4999 1608 552
SalI G'TCGA_C
Cuts at: 1936 4501 1936
Size: 2565 4594
SapI GCTCTTCn'nnn_
Cuts at: 4070 4208 4070
Size: 138 7021
SbfI CC_TGCA'GG
Cuts at: 3873 4511 3873
Size: 638 6521
ScaI AGT'ACT
Cuts at: 2393 3074 5407 7119 2393
Size: 681 2333 1712 2433
Fragments arranged by size:
2433 2333 1712 681
SexAI A'CCwGG_T
Cuts at: 2403 3354 2403
Size: 951 6208
SfcI C'TryA_G
Cuts at: 826 1045 2156 2437 2657 3106 3559 3728 3869
Size: 219 1111 281 220 449 453 169 141
Cuts at: 3869 4142 4250 4507 5642 6323 6514 826
Size: 273 108 257 1135 681 191 1471
Fragments arranged by size:
1471 1135 1111 681 453 449 281 273
257 220 219 191 169 141 108
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7159
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7159
SmaI CCC'GGG
Cuts at: 1872 4486 1872
Size: 2614 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3065 3374 3434 3659 3674 3782
Size: 197 926 1122 309 60 225 15 108
Cuts at: 3782 3884 3899 3953 4358 5263 6164 6426 820
Size: 102 15 54 405 905 901 262 1553
Fragments arranged by size:
1553 1122 926 905 901 405 309 262
225 197 108 102 60 54 15 15
SnaBI TAC'GTA
Cuts at: 493 4456 4950 493
Size: 3963 494 2702
Fragments arranged by size:
3963 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7159
SphI G_CATG'C
Cuts at: 1132 4517 1132
Size: 3385 3774
SspI AAT'ATT
Cuts at: 5 52 5083 5
Size: 47 5031 2081
Fragments arranged by size:
5031 2081 47
StuI AGG'CCT
Cuts at: 1517 4451 1517
Size: 2934 4225
StyI C'CwwG_G
Cuts at: 513 1065 2205 2601 2742 4598 513
Size: 552 1140 396 141 1856 3074
Fragments arranged by size:
3074 1856 1140 552 396 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2180 4073 5186 5203 5356 5541 5986
Size: 1255 600 1893 1113 17 153 185 445
Cuts at: 5986 325
Size: 1498
Fragments arranged by size:
1893 1498 1255 1113 600 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2337 2391 3072
Size: 274 80 33 51 1443 360 54 681
Cuts at: 3072 5405 7117 96
Size: 2333 1712 138
Fragments arranged by size:
2333 1712 1443 681 360 274 138 80
54 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2342 2374 4412 4444 7119 7151 2342
Size: 32 2038 32 2675 32 2350
Fragments arranged by size:
2675 2350 2038 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2668 1316
Size: 1352 5807
XbaI T'CTAG_A
Cuts at: 4495 4495
Size: 7159
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2476 2476
Size: 7159
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7159
XmaI C'CCGG_G
Cuts at: 1870 4484 1870
Size: 2614 4545
XmnI GAAnn'nnTTC
Cuts at: 2510 2768 3134 5288 2510
Size: 258 366 2154 4381
Fragments arranged by size:
4381 2154 366 258
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3124 4967 276
Size: 53 83 186 936 1590 1843 2468
Fragments arranged by size:
2468 1843 1590 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI
Bsu36I EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PspXI RsrII SacII SanDI SfiI
SfoI SrfI SwaI