Restriction map for
pFN21ASDA1394
(Circular) (Six-base) MAPSORT of: pFN21ASDA1394.seq Check: 7461 from: 1 to: 7627
pFN21ASDA1394 7627 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3085 3280 4100 4686 3085
Size: 195 820 586 6026
Fragments arranged by size:
6026 820 586 195
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5437 278
Size: 53 83 186 936 3901 2468
Fragments arranged by size:
3901 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2262 4970 1937
Size: 325 2708 4594
Fragments arranged by size:
4594 2708 325
Acc65I G'GTAC_C
Cuts at: 4072 4948 4072
Size: 876 6751
AclI AA'CG_TT
Cuts at: 1563 5754 6127 1563
Size: 4191 373 3063
Fragments arranged by size:
4191 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5690 7074 7342 1632
Size: 4058 1384 268 1917
Fragments arranged by size:
4058 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7430
AflIII A'CryG_T
Cuts at: 1203 6526 7503 1203
Size: 5323 977 1327
Fragments arranged by size:
5323 1327 977
AgeI A'CCGG_T
Cuts at: 6458 6458
Size: 7627
AhdI GACnn_n'nnGTC
Cuts at: 6356 6356
Size: 7627
AleI CACnn'nnGTG
Cuts at: 1166 4731 1166
Size: 3565 4062
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2414 4250 1184
Size: 1230 1836 4561
Fragments arranged by size:
4561 1836 1230
AlwNI CAG_nnn'CTG
Cuts at: 2728 2943 3628 4678 4687 4723 4867 5370 6942
Size: 215 685 1050 9 36 144 503 1572
Cuts at: 6942 2728
Size: 3413
Fragments arranged by size:
3413 1572 1050 685 503 215 144 36
9
ApaI G_GGCC'C
Cuts at: 2144 4884 2144
Size: 2740 4887
ApaLI G'TGCA_C
Cuts at: 4064 5684 6840 4064
Size: 1620 1156 4851
Fragments arranged by size:
4851 1620 1156
ApoI r'AATT_y
Cuts at: 1493 4936 5162 5314 7423 1493
Size: 3443 226 152 2109 1697
Fragments arranged by size:
3443 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6181 160
Size: 872 5149 1606
Fragments arranged by size:
5149 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7627
AvaI C'yCGr_G
Cuts at: 1870 1943 2096 2231 2247 2431 2486 4176 4952
Size: 73 153 135 16 184 55 1690 776
Cuts at: 4952 1870
Size: 4545
Fragments arranged by size:
4545 1690 776 184 153 135 73 55
16
AvrII C'CTAG_G
Cuts at: 2815 2815
Size: 7627
BamHI G'GATC_C
Cuts at: 2083 2225 4545 4957 5324 2083
Size: 142 2320 412 367 4386
Fragments arranged by size:
4386 2320 412 367 142
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2196 2332 2476 3222 3390
Size: 325 217 627 409 136 144 746 168
Cuts at: 3390 3565 4072 4259 4384 4771 4948 6403 618
Size: 175 507 187 125 387 177 1455 1842
Fragments arranged by size:
1842 1455 746 627 507 409 387 325
217 187 177 175 168 144 136 125
BanII G_rGCy'C
Cuts at: 1391 2120 2144 2159 2384 2432 3693 3826 4473
Size: 729 24 15 225 48 1261 133 647
Cuts at: 4473 4557 4794 4884 4946 1391
Size: 84 237 90 62 4072
Fragments arranged by size:
4072 1261 729 647 237 225 133 90
84 62 48 24 15
BbeI G_GCGC'C
Cuts at: 2200 3394 3569 2200
Size: 1194 175 6258
Fragments arranged by size:
6258 1194 175
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4450 928
Size: 971 2551 4105
Fragments arranged by size:
4105 2551 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3643 3677 5818 5852 1903
Size: 34 1706 34 2141 34 3678
Fragments arranged by size:
3678 2141 1706 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5519 6729 5519
Size: 1210 6417
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7627
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2115 2788 3338
Size: 107 122 71 631 605 443 673 550
Cuts at: 3338 3350 3554 4378 4851 6238 136
Size: 12 204 824 473 1387 1525
Fragments arranged by size:
1525 1387 824 673 631 605 550 473
443 204 122 107 71 12
BglII A'GATC_T
Cuts at: 7622 7622
Size: 7627
BlpI GC'TnA_GC
Cuts at: 2682 3176 3942 5044 2682
Size: 494 766 1102 5265
Fragments arranged by size:
5265 1102 766 494
Bme1580I G_kGCm'C
Cuts at: 1790 2030 2144 2337 4023 4068 4262 4884 5688
Size: 240 114 193 1686 45 194 622 804
Cuts at: 5688 6844 1790
Size: 1156 2573
Fragments arranged by size:
2573 1686 1156 804 622 240 194 193
114 45
BmgBI CAC'GTC
Cuts at: 1318 2206 4273 1318
Size: 888 2067 4672
Fragments arranged by size:
4672 2067 888
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2123 3259 3452
Size: 416 53 83 399 24 706 1136 193
Cuts at: 3452 3730 3868 5358 6316 442
Size: 278 138 1490 958 1753
Fragments arranged by size:
1753 1490 1136 958 706 416 399 278
193 138 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7627
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2150 2432 2720 3047 3056 3269 3377 3488
Size: 911 282 288 327 9 213 108 111
Cuts at: 3488 3512 3530 3677 4055 4118 4571 4778 6287
Size: 24 18 147 378 63 453 207 1509
Cuts at: 6287 1239
Size: 2579
Fragments arranged by size:
2579 1509 911 453 378 327 288 282
213 207 147 111 108 63 24 18
9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3818 4457 5752 6617 6915 3818
Size: 639 1295 865 298 4530
Fragments arranged by size:
4530 1295 865 639 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3572 4703 6290 882
Size: 617 2073 1131 1587 2219
Fragments arranged by size:
2219 2073 1587 1131 617
BsaAI yAC'GTr
Cuts at: 493 1204 4631 4924 5418 493
Size: 711 3427 293 494 2702
Fragments arranged by size:
3427 2702 711 494 293
BsaBI GATnn'nnATC
Cuts at: 7621 7621
Size: 7627
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2197 2971 3391 3566
Size: 53 83 186 936 664 774 420 175
Cuts at: 3566 3648 4653 5434 5816 6451 275
Size: 82 1005 781 382 635 1451
Fragments arranged by size:
1451 1005 936 781 774 664 635 420
382 186 175 83 82 53
BsaWI w'CCGG_w
Cuts at: 2308 3777 6060 6458 6732 6879 7290 2308
Size: 1469 2283 398 274 147 411 2645
Fragments arranged by size:
2645 2283 1469 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3053 3083 3215 3245 3503
Size: 30 165 30 1650 30 132 30 258
Cuts at: 3503 3533 1178
Size: 30 5272
Fragments arranged by size:
5272 1650 258 165 132 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3053 3056 3059 3296 3803 4775 4802 1186
Size: 1867 3 3 237 507 972 27 4011
Fragments arranged by size:
4011 1867 972 507 237 27 3 3
BseYI C'CCAG_C
Cuts at: 2387 2733 2992 3081 3535 3922 3960 4216 4221
Size: 346 259 89 454 387 38 256 5
Cuts at: 4221 4487 4671 4820 4849 4883 6830 7326 2387
Size: 266 184 149 29 34 1947 496 2688
Fragments arranged by size:
2688 1947 496 454 387 346 266 259
256 184 149 89 38 34 29 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2960 3018 3683 3950 4134 4709 1163
Size: 573 1224 58 665 267 184 575 4081
Fragments arranged by size:
4081 1224 665 575 573 267 184 58
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3327 3441 3549 3596 4732 5077
Size: 275 186 1326 114 108 47 1136 345
Cuts at: 5077 5838 6866 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1326 1136 1028 761 345 275 186
114 108 47
BsiHKAI G_wGCw'C
Cuts at: 3777 3879 4068 4161 4269 4473 4557 4636 5688
Size: 102 189 93 108 204 84 79 1052
Cuts at: 5688 5773 6844 3777
Size: 85 1071 4560
Fragments arranged by size:
4560 1071 1052 204 189 108 102 93
85 84 79
BsmI GAATG_Cn'
Cuts at: 5138 5231 5138
Size: 93 7534
BsmBI CGTCTCn'nnnn_
Cuts at: 2852 4349 4844 2852
Size: 1497 495 5635
Fragments arranged by size:
5635 1497 495
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2120 2144 2159 2337 2384 2432
Size: 399 240 90 24 15 178 47 48
Cuts at: 2432 3693 3777 3826 3879 4023 4068 4161 4262
Size: 1261 84 49 53 144 45 93 101
Cuts at: 4262 4269 4473 4557 4636 4794 4884 4946 5688
Size: 7 204 84 79 158 90 62 742
Cuts at: 5688 5773 6844 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1261 1071 742 399 240 204 178
158 144 101 93 90 90 85 84
84 79 62 53 49 48 47 45
24 15 7
BspEI T'CCGG_A
Cuts at: 2308 7290 2308
Size: 4982 2645
BspHI T'CATG_A
Cuts at: 4089 5516 7246 4089
Size: 1427 1730 4470
Fragments arranged by size:
4470 1730 1427
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2669 2911 3085 3280 4100 4542 4578 4686
Size: 1825 242 174 195 820 442 36 108
Cuts at: 4686 4982 844
Size: 296 3489
Fragments arranged by size:
3489 1825 820 442 296 242 195 174
108 36
BsrBI CCG'CTC
Cuts at: 1394 1823 5514 1394
Size: 429 3691 3507
Fragments arranged by size:
3691 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4234 6122 6296 65
Size: 1183 2986 1888 174 1396
Fragments arranged by size:
2986 1888 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2706 3362 3440 3541 6271 6458 1700
Size: 110 896 656 78 101 2730 187 2869
Fragments arranged by size:
2869 2730 896 656 187 110 101 78
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7627
BssHII G'CGCG_C
Cuts at: 1925 3504 3599 3677 1925
Size: 1579 95 78 5875
Fragments arranged by size:
5875 1579 95 78
BssSI C'ACGA_G
Cuts at: 3704 4635 5687 6699 3704
Size: 931 1052 1012 4632
Fragments arranged by size:
4632 1052 1012 931
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3973 4723 794
Size: 3179 750 3698
Fragments arranged by size:
3698 3179 750
BstBI TT'CG_AA
Cuts at: 2007 6431 2007
Size: 4424 3203
BstEII G'GTnAC_C
Cuts at: 1184 2362 1184
Size: 1178 6449
BstXI CCAn_nnnn'nTGG
Cuts at: 2128 2741 3937 4381 2128
Size: 613 1196 444 5374
Fragments arranged by size:
5374 1196 613 444
BstYI r'GATC_y
Cuts at: 1972 2083 2225 4545 4957 5324 5709 5726 7178
Size: 111 142 2320 412 367 385 17 1452
Cuts at: 7178 7264 7276 7622 1972
Size: 86 12 346 1977
Fragments arranged by size:
2320 1977 1452 412 385 367 346 142
111 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2323 2981 3202 3530 3982 4732 513
Size: 552 1258 658 221 328 452 750 3408
Fragments arranged by size:
3408 1258 750 658 552 452 328 221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6888
Fragments arranged by size:
6888 683 56
BtsI GCAGTG_nn'
Cuts at: 3006 3170 4122 4807 5143 5937 5957 3006
Size: 164 952 685 336 794 20 4676
Fragments arranged by size:
4676 952 794 685 336 164 20
ClaI AT'CG_AT
Cuts at: 1554 4875 1554
Size: 3321 4306
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7592
DraI TTT'AAA
Cuts at: 4931 4931
Size: 7627
DraIII CAC_nnn'GTG
Cuts at: 3161 3706 4273 3161
Size: 545 567 6515
Fragments arranged by size:
6515 567 545
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6634 809
Size: 507 5318 1802
Fragments arranged by size:
5318 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2326 2620 3123
Size: 54 1425 50 275 18 496 294 503
Cuts at: 3123 3324 3339 3438 3546 4639 5074 5963 7592
Size: 201 15 99 108 1093 435 889 1629
Cuts at: 7592 8
Size: 43
Fragments arranged by size:
1629 1425 1093 889 503 496 435 294
275 201 108 99 54 50 43 18
15
EagI C'GGCC_G
Cuts at: 1537 1812 3324 3438 3546 5074 1537
Size: 275 1512 114 108 1528 4090
Fragments arranged by size:
4090 1528 1512 275 114 108
EarI CTCTTCn'nnn_
Cuts at: 1353 2130 3944 5557 1353
Size: 777 1814 1613 3423
Fragments arranged by size:
3423 1814 1613 777
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2372 4832 6214 6588 6734 387
Size: 1064 138 783 2460 1382 374 146 1280
Fragments arranged by size:
2460 1382 1280 1064 783 374 146 138
EcoICRI GAG'CTC
Cuts at: 4471 4555 4471
Size: 84 7543
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2150 2432 2720 3047 3056 3269 3377
Size: 393 518 282 288 327 9 213 108
Cuts at: 3377 3488 3512 3530 3677 4055 4118 4571 4778
Size: 111 24 18 147 378 63 453 207
Cuts at: 4778 5690 6287 7074 7342 1239
Size: 912 597 787 268 1524
Fragments arranged by size:
1524 912 787 597 518 453 393 378
327 288 282 268 213 207 147 111
108 63 24 18 9
EcoNI CCTnn'n_nnAGG
Cuts at: 3059 3278 3611 4859 3059
Size: 219 333 1248 5827
Fragments arranged by size:
5827 1248 333 219
EcoO109I rG'GnC_Cy
Cuts at: 2093 2125 2140 2141 2545 2615 2902 4684 4720
Size: 32 15 1 404 70 287 1782 36
Cuts at: 4720 4765 4864 2093
Size: 45 99 4856
Fragments arranged by size:
4856 1782 404 287 99 70 45 36
32 15 1
EcoRI G'AATT_C
Cuts at: 4936 5314 4936
Size: 378 7249
EcoRV GAT'ATC
Cuts at: 2914 2914
Size: 7627
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2321 2321
Size: 7627
FseI GG_CCGG'CC
Cuts at: 3366 3366
Size: 7627
FspI TGC'GCA
Cuts at: 6133 6133
Size: 7627
HaeII r_GCGC'y
Cuts at: 1678 2200 2950 3023 3260 3394 3569 3642 6774
Size: 522 750 73 237 134 175 73 3132
Cuts at: 6774 1678
Size: 2531
Fragments arranged by size:
3132 2531 750 522 237 175 134 73
73
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 6268 6300 6342 6374 1373
Size: 32 135 32 4696 32 42 32 2626
Fragments arranged by size:
4696 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4567 4971 5217 669
Size: 988 281 2629 404 246 3079
Fragments arranged by size:
3079 2629 988 404 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7627
HpaI GTT'AAC
Cuts at: 5217 5217
Size: 7627
KasI G'GCGC_C
Cuts at: 2196 3390 3565 2196
Size: 1194 175 6258
Fragments arranged by size:
6258 1194 175
KpnI G_GTAC'C
Cuts at: 4076 4952 4076
Size: 876 6751
MfeI C'AATT_G
Cuts at: 5226 5226
Size: 7627
MluI A'CGCG_T
Cuts at: 7503 7503
Size: 7627
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3131 4344 6741 6925 1305
Size: 1826 1213 2397 184 2007
Fragments arranged by size:
2397 2007 1826 1213 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4641 10
Size: 54 1425 343 2809 2996
Fragments arranged by size:
2996 2809 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3981 4731 5585 5944 6103 518
Size: 648 2815 750 854 359 159 2042
Fragments arranged by size:
2815 2042 854 750 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2325 2983 3085 3204 3437 3532 3571 3625
Size: 719 658 102 119 233 95 39 54
Cuts at: 3625 3697 3926 3964 4425 5043 5720 6868 7113
Size: 72 229 38 461 618 677 1148 245
Cuts at: 7113 1606
Size: 2120
Fragments arranged by size:
2120 1148 719 677 658 618 461 245
233 229 119 102 95 72 54 39
38
NaeI GCC'GGC
Cuts at: 3364 3442 3543 3364
Size: 78 101 7448
Fragments arranged by size:
7448 101 78
NarI GG'CG_CC
Cuts at: 2197 3391 3566 2197
Size: 1194 175 6258
Fragments arranged by size:
6258 1194 175
NcoI C'CATG_G
Cuts at: 513 1065 3982 513
Size: 552 2917 4158
Fragments arranged by size:
4158 2917 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7627
NgoMIV G'CCGG_C
Cuts at: 3362 3440 3541 3362
Size: 78 101 7448
Fragments arranged by size:
7448 101 78
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7627
NotI GC'GGCC_GC
Cuts at: 5074 5074
Size: 7627
NspI r_CATG'y
Cuts at: 1132 1229 2443 4372 4985 6530 1132
Size: 97 1214 1929 613 1545 2229
Fragments arranged by size:
2229 1929 1545 1214 613 97
PasI CC'CwG_GG
Cuts at: 1348 2145 2540 2905 1348
Size: 797 395 365 6070
Fragments arranged by size:
6070 797 395 365
PciI A'CATG_T
Cuts at: 6526 6526
Size: 7627
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2206 4211 4648 4867 1108
Size: 1098 2005 437 219 3868
Fragments arranged by size:
3868 2005 1098 437 219
PfoI T'CCnGG_A
Cuts at: 2556 4166 4548 2556
Size: 1610 382 5635
Fragments arranged by size:
5635 1610 382
PmeI GTTT'AAAC
Cuts at: 4931 4931
Size: 7627
PmlI CAC'GTG
Cuts at: 4631 4631
Size: 7627
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5696 5728 7209 7241 5696
Size: 32 1481 32 6082
Fragments arranged by size:
6082 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2093 2125 2093
Size: 32 7595
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7627
PsiI TTA'TAA
Cuts at: 5197 5197
Size: 7627
PspOMI G'GGCC_C
Cuts at: 2140 4880 2140
Size: 2740 4887
PstI C_TGCA'G
Cuts at: 830 2283 2295 2922 3917 4683 4719 4802 4979
Size: 1453 12 627 995 766 36 83 177
Cuts at: 4979 830
Size: 3478
Fragments arranged by size:
3478 1453 995 766 627 177 83 36
12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7627
PvuII CAG'CTG
Cuts at: 3085 3625 3697 3926 3964 3085
Size: 540 72 229 38 6748
Fragments arranged by size:
6748 540 229 72 38
SacI G_AGCT'C
Cuts at: 4473 4557 4473
Size: 84 7543
SacII CC_GC'GG
Cuts at: 2326 2984 3205 3533 2326
Size: 658 221 328 6420
Fragments arranged by size:
6420 658 328 221
SalI G'TCGA_C
Cuts at: 1936 4969 1936
Size: 3033 4594
SanDI GG'GwC_CC
Cuts at: 2093 2093
Size: 7627
SapI GCTCTTCn'nnn_
Cuts at: 3944 3944
Size: 7627
SbfI CC_TGCA'GG
Cuts at: 2922 4683 4719 4979 2922
Size: 1761 36 260 5570
Fragments arranged by size:
5570 1761 260 36
ScaI AGT'ACT
Cuts at: 5875 7587 5875
Size: 1712 5915
SexAI A'CCwGG_T
Cuts at: 4068 4278 4068
Size: 210 7417
SfcI C'TryA_G
Cuts at: 826 1045 2269 2279 2291 2918 3913 4679 4715
Size: 219 1224 10 12 627 995 766 36
Cuts at: 4715 4798 4975 6110 6791 6982 826
Size: 83 177 1135 681 191 1471
Fragments arranged by size:
1471 1224 1135 995 766 681 627 219
191 177 83 36 12 10
SfiI GGCCn_nnn'nGGCC
Cuts at: 3338 3338
Size: 7627
SfoI GGC'GCC
Cuts at: 2198 3392 3567 2198
Size: 1194 175 6258
Fragments arranged by size:
6258 1194 175
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7627
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7627
SmaI CCC'GGG
Cuts at: 1872 2249 2433 2488 4954 1872
Size: 377 184 55 2466 4545
Fragments arranged by size:
4545 2466 377 184 55
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3833 4472 5731 6632 6894 820
Size: 197 926 1890 639 1259 901 262 1553
Fragments arranged by size:
1890 1553 1259 926 901 639 262 197
SnaBI TAC'GTA
Cuts at: 493 4924 5418 493
Size: 4431 494 2702
Fragments arranged by size:
4431 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7627
SphI G_CATG'C
Cuts at: 1132 4372 4985 1132
Size: 3240 613 3774
Fragments arranged by size:
3774 3240 613
SrfI GCCC'GGGC
Cuts at: 2249 2249
Size: 7627
SspI AAT'ATT
Cuts at: 5 52 5551 5
Size: 47 5499 2081
Fragments arranged by size:
5499 2081 47
StuI AGG'CCT
Cuts at: 1517 2604 1517
Size: 1087 6540
StyI C'CwwG_G
Cuts at: 513 1065 2121 2520 2815 3982 5066 513
Size: 552 1056 399 295 1167 1084 3074
Fragments arranged by size:
3074 1167 1084 1056 552 399 295
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2897 2984 3974 4109 4218 4511 5654
Size: 1255 1317 87 990 135 109 293 1143
Cuts at: 5654 5671 5824 6009 6454 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1317 1255 1143 990 445 293 185
153 135 109 87 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5873 7585 96
Size: 274 80 33 51 1443 3896 1712 138
Fragments arranged by size:
3896 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2332 2364 3145 3177 3215 3247 3503 3535 3716
Size: 32 781 32 38 32 256 32 181
Cuts at: 3716 3748 7587 7619 2332
Size: 32 3839 32 2340
Fragments arranged by size:
3839 2340 781 256 181 38 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2567 1316
Size: 1251 6376
XbaI T'CTAG_A
Cuts at: 4963 4963
Size: 7627
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 2593 4208 4240 2029
Size: 564 1615 32 5416
Fragments arranged by size:
5416 1615 564 32
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7627
XmaI C'CCGG_G
Cuts at: 1870 2247 2431 2486 4952 1870
Size: 377 184 55 2466 4545
Fragments arranged by size:
4545 2466 377 184 55
XmnI GAAnn'nnTTC
Cuts at: 5756 5756
Size: 7627
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5435 276
Size: 53 83 186 936 3901 2468
Fragments arranged by size:
3901 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BstZ17I Bsu36I FspAI NruI NsiI PacI PspXI
PsrI RsrII SwaI