Restriction map for
pFN21ASDA1392
(Circular) (Six-base) MAPSORT of: pFN21ASDA1392.seq Check: 5703 from: 1 to: 7507
pFN21ASDA1392 7507 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4222 4222
Size: 7507
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5317 278
Size: 53 83 186 936 3781 2468
Fragments arranged by size:
3781 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4850 1937
Size: 2913 4594
Acc65I G'GTAC_C
Cuts at: 2493 4828 2493
Size: 2335 5172
AclI AA'CG_TT
Cuts at: 1563 5634 6007 1563
Size: 4071 373 3063
Fragments arranged by size:
4071 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2919 3582 4059 5570 6954 7222 1632
Size: 1287 663 477 1511 1384 268 1917
Fragments arranged by size:
1917 1511 1384 1287 663 477 268
AfeI AGC'GCT
Cuts at: 3727 3727
Size: 7507
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7310
AflIII A'CryG_T
Cuts at: 1203 6406 7383 1203
Size: 5203 977 1327
Fragments arranged by size:
5203 1327 977
AgeI A'CCGG_T
Cuts at: 2520 6338 2520
Size: 3818 3689
AhdI GACnn_n'nnGTC
Cuts at: 3454 3639 4061 6236 3454
Size: 185 422 2175 4725
Fragments arranged by size:
4725 2175 422 185
AleI CACnn'nnGTG
Cuts at: 1166 3305 4074 1166
Size: 2139 769 4599
Fragments arranged by size:
4599 2139 769
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2063 4092 1184
Size: 879 2029 4599
Fragments arranged by size:
4599 2029 879
AlwNI CAG_nnn'CTG
Cuts at: 5250 6822 5250
Size: 1572 5935
ApaI G_GGCC'C
Cuts at: 4016 4016
Size: 7507
ApaLI G'TGCA_C
Cuts at: 2530 2639 3422 5564 6720 2530
Size: 109 783 2142 1156 3317
Fragments arranged by size:
3317 2142 1156 783 109
ApoI r'AATT_y
Cuts at: 1493 2364 2755 2816 4472 4816 5042 5194 7303
Size: 871 391 61 1656 344 226 152 2109
Cuts at: 7303 1493
Size: 1697
Fragments arranged by size:
2109 1697 1656 871 391 344 226 152
61
AseI AT'TA_AT
Cuts at: 160 1032 6061 160
Size: 872 5029 1606
Fragments arranged by size:
5029 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7507
AvaI C'yCGr_G
Cuts at: 1870 1943 2243 2546 2943 3311 3758 4439 4611
Size: 73 300 303 397 368 447 681 172
Cuts at: 4611 4832 1870
Size: 221 4545
Fragments arranged by size:
4545 681 447 397 368 303 300 221
172 73
BamHI G'GATC_C
Cuts at: 4837 5204 4837
Size: 367 7140
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2493 2722 3238 3859 4076
Size: 325 217 627 706 229 516 621 217
Cuts at: 4076 4421 4828 6283 618
Size: 345 407 1455 1842
Fragments arranged by size:
1842 1455 706 627 621 516 407 345
325 229 217 217
BanII G_rGCy'C
Cuts at: 1391 3655 4016 4086 4682 4734 4826 1391
Size: 2264 361 70 596 52 92 4072
Fragments arranged by size:
4072 2264 596 361 92 70 52
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2036 2640 3558 4214 928
Size: 971 137 604 918 656 4221
Fragments arranged by size:
4221 971 918 656 604 137
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2262 2296 2589 2623 5698 5732 1903
Size: 34 325 34 293 34 3075 34 3678
Fragments arranged by size:
3678 3075 325 293 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2505 5399 6609 2505
Size: 2894 1210 3403
Fragments arranged by size:
3403 2894 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7507
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3976 6118 136
Size: 107 122 71 631 605 2304 2142 1525
Fragments arranged by size:
2304 2142 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2406 7502 2406
Size: 5096 2411
BlpI GC'TnA_GC
Cuts at: 4924 4924
Size: 7507
Bme1580I G_kGCm'C
Cuts at: 1790 2534 2643 3243 3426 3981 4016 5568 6724
Size: 744 109 600 183 555 35 1552 1156
Cuts at: 6724 1790
Size: 2573
Fragments arranged by size:
2573 1552 1156 744 600 555 183 109
35
BmgBI CAC'GTC
Cuts at: 1318 2638 1318
Size: 1320 6187
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2881 5238 6196
Size: 416 53 83 399 24 1464 2357 958
Cuts at: 6196 442
Size: 1753
Fragments arranged by size:
2357 1753 1464 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7507
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3123 3123
Size: 7507
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2403 3946 6167 1239
Size: 1164 1543 2221 2579
Fragments arranged by size:
2579 2221 1543 1164
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5632 6497 6795 5632
Size: 865 298 6344
Fragments arranged by size:
6344 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2529 3141 3159 6170 882
Size: 617 1030 612 18 3011 2219
Fragments arranged by size:
3011 2219 1030 617 612 18
BsaAI yAC'GTr
Cuts at: 493 1204 2331 4607 4804 5298 493
Size: 711 1127 2276 197 494 2702
Fragments arranged by size:
2702 2276 1127 711 494 197
BsaBI GATnn'nnATC
Cuts at: 2960 7501 2960
Size: 4541 2966
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3545 4057 5314 5696
Size: 53 83 186 936 2012 512 1257 382
Cuts at: 5696 6331 275
Size: 635 1451
Fragments arranged by size:
2012 1451 1257 936 635 512 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2520 2835 3450 5940 6338 6612 6759 7170 2520
Size: 315 615 2490 398 274 147 411 2857
Fragments arranged by size:
2857 2490 615 411 398 315 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7282
Fragments arranged by size:
7282 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3067 3949 4001 4044 4181 4409 4412 4427
Size: 1881 882 52 43 137 228 3 15
Cuts at: 4427 1186
Size: 4266
Fragments arranged by size:
4266 1881 882 228 137 52 43 15
3
BseYI C'CCAG_C
Cuts at: 3196 3590 4085 4293 6710 7206 3196
Size: 394 495 208 2417 496 3497
Fragments arranged by size:
3497 2417 496 495 394 208
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2114 3612 1163
Size: 573 378 1498 5058
Fragments arranged by size:
5058 1498 573 378
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4957 5718 6746 1540
Size: 275 186 2956 761 1028 2301
Fragments arranged by size:
2956 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2042 2322 2534 2643 3312 3426 3855 5568 5653
Size: 280 212 109 669 114 429 1713 85
Cuts at: 5653 6724 2042
Size: 1071 2825
Fragments arranged by size:
2825 1713 1071 669 429 280 212 114
109 85
BsmI GAATG_Cn'
Cuts at: 5018 5111 5018
Size: 93 7414
BsmBI CGTCTCn'nnnn_
Cuts at: 2910 3839 4354 2910
Size: 929 515 6063
Fragments arranged by size:
6063 929 515
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2322 2534 2643 3243 3312 3426
Size: 399 252 280 212 109 600 69 114
Cuts at: 3426 3655 3855 3981 4016 4086 4682 4734 4826
Size: 229 200 126 35 70 596 52 92
Cuts at: 4826 5568 5653 6724 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 600 596 399 280 252
229 212 200 126 114 109 92 85
70 69 52 35
BspEI T'CCGG_A
Cuts at: 7170 7170
Size: 7507
BspHI T'CATG_A
Cuts at: 3021 5396 7126 3021
Size: 2375 1730 3402
Fragments arranged by size:
3402 2375 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3937 4222 4862 844
Size: 3093 285 640 3489
Fragments arranged by size:
3489 3093 640 285
BsrBI CCG'CTC
Cuts at: 1394 1823 4444 5394 1394
Size: 429 2621 950 3507
Fragments arranged by size:
3507 2621 950 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2280 2591 4223 6002 6176 65
Size: 1183 1032 311 1632 1779 174 1396
Fragments arranged by size:
1779 1632 1396 1183 1032 311 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2520 6151 6338 1700
Size: 110 710 3631 187 2869
Fragments arranged by size:
3631 2869 710 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7507
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7507
BssSI C'ACGA_G
Cuts at: 5567 6579 5567
Size: 1012 6495
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7507
BstBI TT'CG_AA
Cuts at: 2007 6311 2007
Size: 4304 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7507
BstXI CCAn_nnnn'nTGG
Cuts at: 2869 3502 4073 4093 2869
Size: 633 571 20 6283
Fragments arranged by size:
6283 633 571 20
BstYI r'GATC_y
Cuts at: 1972 2250 2406 3470 3491 4837 5204 5589 5606
Size: 278 156 1064 21 1346 367 385 17
Cuts at: 5606 7058 7144 7156 7502 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1346 1064 385 367 346 278
156 86 21 17 12
Bsu36I CC'TnA_GG
Cuts at: 2569 2569
Size: 7507
BtgI C'CryG_G
Cuts at: 513 1065 2145 2991 3121 4072 4687 513
Size: 552 1080 846 130 951 615 3333
Fragments arranged by size:
3333 1080 951 846 615 552 130
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6768
Fragments arranged by size:
6768 683 56
BtsI GCAGTG_nn'
Cuts at: 2605 2620 4436 5023 5817 5837 2605
Size: 15 1816 587 794 20 4275
Fragments arranged by size:
4275 1816 794 587 20 15
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7507
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7472
DraI TTT'AAA
Cuts at: 2848 4811 2848
Size: 1963 5544
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3154 6514 809
Size: 507 1838 3360 1802
Fragments arranged by size:
3360 1838 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2262 2871 4954
Size: 54 1425 50 275 18 432 609 2083
Cuts at: 4954 5843 7472 8
Size: 889 1629 43
Fragments arranged by size:
2083 1629 1425 889 609 432 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4954 1537
Size: 275 3142 4090
Fragments arranged by size:
4090 3142 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2895 4146 5437 1353
Size: 1542 1251 1291 3423
Fragments arranged by size:
3423 1542 1291 1251
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6094 6468 6614 387
Size: 1064 138 4505 374 146 1280
Fragments arranged by size:
4505 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2403 2919 3582 3946 4059 5570 6167
Size: 393 771 516 663 364 113 1511 597
Cuts at: 6167 6954 7222 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1511 787 771 663 597 516 393
364 268 113
EcoO109I rG'GnC_Cy
Cuts at: 2103 3066 3518 3949 4012 4013 4263 2103
Size: 963 452 431 63 1 250 5347
Fragments arranged by size:
5347 963 452 431 250 63 1
EcoRI G'AATT_C
Cuts at: 4816 5194 4816
Size: 378 7129
EcoRV GAT'ATC
Cuts at: 3193 3193
Size: 7507
FspI TGC'GCA
Cuts at: 6013 6013
Size: 7507
HaeII r_GCGC'y
Cuts at: 1678 3729 6654 1678
Size: 2051 2925 2531
Fragments arranged by size:
2925 2531 2051
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2072 2104 2761 2793 3440
Size: 32 135 32 500 32 657 32 647
Cuts at: 3440 3472 3625 3657 4047 4079 4349 4381 6148
Size: 32 153 32 390 32 270 32 1767
Cuts at: 6148 6180 6222 6254 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1767 657 647 500 390 270 153
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4851 5097 669
Size: 988 281 2913 246 3079
Fragments arranged by size:
3079 2913 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4782 748
Size: 4034 3473
HpaI GTT'AAC
Cuts at: 5097 5097
Size: 7507
KpnI G_GTAC'C
Cuts at: 2497 4832 2497
Size: 2335 5172
MfeI C'AATT_G
Cuts at: 5106 5106
Size: 7507
MluI A'CGCG_T
Cuts at: 7383 7383
Size: 7507
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2372 3198 3498 3821 4653 4794 6621 6805
Size: 1067 826 300 323 832 141 1827 184
Cuts at: 6805 1305
Size: 2007
Fragments arranged by size:
2007 1827 1067 832 826 323 300 184
141
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2264 2873 10
Size: 54 1425 343 432 609 4644
Fragments arranged by size:
4644 1425 609 432 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2207 3305 3337 4074 4580 5465 5824
Size: 648 1041 1098 32 737 506 885 359
Cuts at: 5824 5983 518
Size: 159 2042
Fragments arranged by size:
2042 1098 1041 885 737 648 506 359
159 32
MspA1I CmG'CkG
Cuts at: 1606 2147 2869 3084 3200 4019 4923 5600 6748
Size: 541 722 215 116 819 904 677 1148
Cuts at: 6748 6993 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 904 819 722 677 541 245
215 116
NcoI C'CATG_G
Cuts at: 513 1065 3121 4072 4687 513
Size: 552 2056 951 615 3333
Fragments arranged by size:
3333 2056 951 615 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7507
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7507
NotI GC'GGCC_GC
Cuts at: 4954 4954
Size: 7507
NspI r_CATG'y
Cuts at: 1132 1229 2654 4865 6410 1132
Size: 97 1425 2211 1545 2229
Fragments arranged by size:
2229 2211 1545 1425 97
PasI CC'CwG_GG
Cuts at: 1348 3243 4258 1348
Size: 1895 1015 4597
Fragments arranged by size:
4597 1895 1015
PciI A'CATG_T
Cuts at: 6406 6406
Size: 7507
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3776 4431 1108
Size: 2668 655 4184
Fragments arranged by size:
4184 2668 655
PfoI T'CCnGG_A
Cuts at: 3458 3458
Size: 7507
PmeI GTTT'AAAC
Cuts at: 4811 4811
Size: 7507
PmlI CAC'GTG
Cuts at: 2331 2331
Size: 7507
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5576 5608 7089 7121 5576
Size: 32 1481 32 5962
Fragments arranged by size:
5962 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2103 3066 3949 4263 2103
Size: 963 883 314 5347
Fragments arranged by size:
5347 963 883 314
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7507
PsiI TTA'TAA
Cuts at: 5077 5077
Size: 7507
PspOMI G'GGCC_C
Cuts at: 4012 4012
Size: 7507
PspXI vC'TCGA_Gb
Cuts at: 3311 4439 3311
Size: 1128 6379
PstI C_TGCA'G
Cuts at: 830 2630 3572 4859 830
Size: 1800 942 1287 3478
Fragments arranged by size:
3478 1800 1287 942
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7507
PvuII CAG'CTG
Cuts at: 2869 3200 2869
Size: 331 7176
RsrII CG'GwC_CG
Cuts at: 2080 2092 2080
Size: 12 7495
SacII CC_GC'GG
Cuts at: 2148 2148
Size: 7507
SalI G'TCGA_C
Cuts at: 1936 4849 1936
Size: 2913 4594
SbfI CC_TGCA'GG
Cuts at: 4859 4859
Size: 7507
ScaI AGT'ACT
Cuts at: 2749 3616 5755 7467 2749
Size: 867 2139 1712 2789
Fragments arranged by size:
2789 2139 1712 867
SfcI C'TryA_G
Cuts at: 826 1045 2626 3568 4757 4855 5990 6671 6862
Size: 219 1581 942 1189 98 1135 681 191
Cuts at: 6862 826
Size: 1471
Fragments arranged by size:
1581 1471 1189 1135 942 681 219 191
98
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7507
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7507
SmaI CCC'GGG
Cuts at: 1872 2548 4834 1872
Size: 676 2286 4545
Fragments arranged by size:
4545 2286 676
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3311 4439 5611 6512 6774 820
Size: 197 926 1368 1128 1172 901 262 1553
Fragments arranged by size:
1553 1368 1172 1128 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4804 5298 493
Size: 4311 494 2702
Fragments arranged by size:
4311 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7507
SphI G_CATG'C
Cuts at: 1132 2654 4865 1132
Size: 1522 2211 3774
Fragments arranged by size:
3774 2211 1522
SspI AAT'ATT
Cuts at: 5 52 5431 5
Size: 47 5379 2081
Fragments arranged by size:
5379 2081 47
StuI AGG'CCT
Cuts at: 1517 3625 1517
Size: 2108 5399
StyI C'CwwG_G
Cuts at: 513 1065 2679 3121 3483 4072 4687 4946 513
Size: 552 1614 442 362 589 615 259 3074
Fragments arranged by size:
3074 1614 615 589 552 442 362 259
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2215 2315 2671 2743 3123 3515 4553
Size: 1255 635 100 356 72 380 392 1038
Cuts at: 4553 5534 5551 5704 5889 6334 325
Size: 981 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1038 981 635 445 392 380
356 185 153 100 72 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2747 3614 5753
Size: 274 80 33 51 1443 770 867 2139
Cuts at: 5753 7465 96
Size: 1712 138
Fragments arranged by size:
2139 1712 1443 867 770 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3647 3679 7467 7499 3647
Size: 32 3788 32 3655
Fragments arranged by size:
3788 3655 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7507
XbaI T'CTAG_A
Cuts at: 4843 4843
Size: 7507
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3520 3970 4694 3520
Size: 450 724 6333
Fragments arranged by size:
6333 724 450
XhoI C'TCGA_G
Cuts at: 1943 3311 4439 1943
Size: 1368 1128 5011
Fragments arranged by size:
5011 1368 1128
XmaI C'CCGG_G
Cuts at: 1870 2546 4832 1870
Size: 676 2286 4545
Fragments arranged by size:
4545 2286 676
XmnI GAAnn'nnTTC
Cuts at: 5636 5636
Size: 7507
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5315 276
Size: 53 83 186 936 3781 2468
Fragments arranged by size:
3781 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII RsrII SacII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PsrI
SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI