Restriction map for
pFN21ASDA1387
(Circular) (Six-base) MAPSORT of: pFN21ASDA1387.seq Check: 3692 from: 1 to: 7276
pFN21ASDA1387 7276 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5086 278
Size: 53 83 186 936 3550 2468
Fragments arranged by size:
3550 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4619 1937
Size: 2682 4594
Acc65I G'GTAC_C
Cuts at: 4597 4597
Size: 7276
AclI AA'CG_TT
Cuts at: 1563 5403 5776 1563
Size: 3840 373 3063
Fragments arranged by size:
3840 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2394 2607 2985 3453 5339 6723 6991 1632
Size: 762 213 378 468 1886 1384 268 1917
Fragments arranged by size:
1917 1886 1384 762 468 378 268 213
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7079
AflIII A'CryG_T
Cuts at: 1203 2429 6175 7152 1203
Size: 1226 3746 977 1327
Fragments arranged by size:
3746 1327 1226 977
AgeI A'CCGG_T
Cuts at: 6107 6107
Size: 7276
AhdI GACnn_n'nnGTC
Cuts at: 6005 6005
Size: 7276
AleI CACnn'nnGTG
Cuts at: 1166 2114 1166
Size: 948 6328
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7276
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3309 3341 3309
Size: 32 7244
AlwNI CAG_nnn'CTG
Cuts at: 2792 4090 5019 6591 2792
Size: 1298 929 1572 3477
Fragments arranged by size:
3477 1572 1298 929
ApaLI G'TGCA_C
Cuts at: 5333 6489 5333
Size: 1156 6120
ApoI r'AATT_y
Cuts at: 1493 2310 2741 2878 3111 3197 3404 3412 3760
Size: 817 431 137 233 86 207 8 348
Cuts at: 3760 3830 3919 3944 4340 4585 4811 4963 7072
Size: 70 89 25 396 245 226 152 2109
Cuts at: 7072 1493
Size: 1697
Fragments arranged by size:
2109 1697 817 431 396 348 245 233
226 207 152 137 89 86 70 25
8
AseI AT'TA_AT
Cuts at: 160 1032 2228 3270 5830 160
Size: 872 1196 1042 2560 1606
Fragments arranged by size:
2560 1606 1196 1042 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7276
AvaI C'yCGr_G
Cuts at: 1870 1943 4601 1870
Size: 73 2658 4545
Fragments arranged by size:
4545 2658 73
BamHI G'GATC_C
Cuts at: 4427 4606 4973 4427
Size: 179 367 6730
Fragments arranged by size:
6730 367 179
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2090 4210 4597 6052 618
Size: 325 217 627 303 2120 387 1455 1842
Fragments arranged by size:
2120 1842 1455 627 387 325 303 217
BanII G_rGCy'C
Cuts at: 1391 2127 4595 1391
Size: 736 2468 4072
Fragments arranged by size:
4072 2468 736
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2073 3480 4055 4302 928
Size: 971 174 1407 575 247 3902
Fragments arranged by size:
3902 1407 971 575 247 174
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3996 4030 5467 5501 1903
Size: 34 2059 34 1437 34 3678
Fragments arranged by size:
3678 2059 1437 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2023 2215 5168 6378 2023
Size: 192 2953 1210 2921
Fragments arranged by size:
2953 2921 1210 192
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7276
BfrBI ATG'CAT
Cuts at: 3083 3436 3083
Size: 353 6923
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5887 136
Size: 107 122 71 631 605 4215 1525
Fragments arranged by size:
4215 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2298 7271 2298
Size: 4973 2303
BlpI GC'TnA_GC
Cuts at: 2721 3240 4693 2721
Size: 519 1453 5304
Fragments arranged by size:
5304 1453 519
Bme1580I G_kGCm'C
Cuts at: 1790 2100 3793 5337 6493 1790
Size: 310 1693 1544 1156 2573
Fragments arranged by size:
2573 1693 1544 1156 310
BmgBI CAC'GTC
Cuts at: 1318 2101 1318
Size: 783 6493
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5007 5965 442
Size: 416 53 83 399 24 3590 958 1753
Fragments arranged by size:
3590 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7276
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5936 1239
Size: 4697 2579
Bpu10I CC'TnA_GC
Cuts at: 3309 4227 3309
Size: 918 6358
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2111 3567 5401 6266 6564 2111
Size: 1456 1834 865 298 2823
Fragments arranged by size:
2823 1834 1456 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5939 882
Size: 617 4440 2219
Fragments arranged by size:
4440 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2116 2430 4573 5067 493
Size: 711 912 314 2143 494 2702
Fragments arranged by size:
2702 2143 912 711 494 314
BsaBI GATnn'nnATC
Cuts at: 3303 3633 7270 3303
Size: 330 3637 3309
Fragments arranged by size:
3637 3309 330
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5083 5465 6100 275
Size: 53 83 186 936 3550 382 635 1451
Fragments arranged by size:
3550 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5709 6107 6381 6528 6939 5709
Size: 398 274 147 411 6046
Fragments arranged by size:
6046 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2090 2120 3309 3339 1178
Size: 30 165 30 687 30 1189 30 5115
Fragments arranged by size:
5115 1189 687 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2095 2135 2473 1186
Size: 909 40 338 5989
Fragments arranged by size:
5989 909 338 40
BseYI C'CCAG_C
Cuts at: 6479 6975 6479
Size: 496 6780
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6703
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2086 4726 5487 6515 1540
Size: 275 186 85 2640 761 1028 2301
Fragments arranged by size:
2640 2301 1028 761 275 186 85
BsiHKAI G_wGCw'C
Cuts at: 2127 2481 5337 5422 6493 2127
Size: 354 2856 85 1071 2910
Fragments arranged by size:
2910 2856 1071 354 85
BsmI GAATG_Cn'
Cuts at: 4787 4880 4787
Size: 93 7183
BsmBI CGTCTCn'nnnn_
Cuts at: 2107 2107
Size: 7276
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2127 2481 3793 4595 5337 5422
Size: 399 310 27 354 1312 802 742 85
Cuts at: 5422 6493 1391
Size: 1071 2174
Fragments arranged by size:
2174 1312 1071 802 742 399 354 310
85 27
BspEI T'CCGG_A
Cuts at: 6939 6939
Size: 7276
BspHI T'CATG_A
Cuts at: 5165 6895 5165
Size: 1730 5546
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2430 4631 844
Size: 1586 2201 3489
Fragments arranged by size:
3489 2201 1586
BsrBI CCG'CTC
Cuts at: 1394 1823 5163 1394
Size: 429 3340 3507
Fragments arranged by size:
3507 3340 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2081 5771 5945 65
Size: 1183 833 3690 174 1396
Fragments arranged by size:
3690 1396 1183 833 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2070 5920 6107 1700
Size: 110 260 3850 187 2869
Fragments arranged by size:
3850 2869 260 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7276
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7276
BssSI C'ACGA_G
Cuts at: 5336 6348 5336
Size: 1012 6264
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7276
BstBI TT'CG_AA
Cuts at: 2007 6080 2007
Size: 4073 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7276
BstXI CCAn_nnnn'nTGG
Cuts at: 2116 2116
Size: 7276
BstYI r'GATC_y
Cuts at: 1972 2298 4427 4606 4973 5358 5375 6827 6913
Size: 326 2129 179 367 385 17 1452 86
Cuts at: 6913 6925 7271 1972
Size: 12 346 1977
Fragments arranged by size:
2129 1977 1452 385 367 346 326 179
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6724
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6537
Fragments arranged by size:
6537 683 56
BtsI GCAGTG_nn'
Cuts at: 4792 5586 5606 4792
Size: 794 20 6462
Fragments arranged by size:
6462 794 20
ClaI AT'CG_AT
Cuts at: 1554 4016 1554
Size: 2462 4814
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7241
DraI TTT'AAA
Cuts at: 3501 4580 3501
Size: 1079 6197
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6283 809
Size: 507 4967 1802
Fragments arranged by size:
4967 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4723 5612 7241
Size: 54 1425 50 275 18 2893 889 1629
Cuts at: 7241 8
Size: 43
Fragments arranged by size:
2893 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4723 1537
Size: 275 2911 4090
Fragments arranged by size:
4090 2911 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2445 2868 3574 3736 4146 4182 4438 4509
Size: 1092 423 706 162 410 36 256 71
Cuts at: 4509 5206 1353
Size: 697 3423
Fragments arranged by size:
3423 1092 706 697 423 410 256 162
71 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3823 5863 6237 6383 387
Size: 1064 138 2234 2040 374 146 1280
Fragments arranged by size:
2234 2040 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2125 2125
Size: 7276
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2394 2607 2985 3453 5339 5936 6723
Size: 393 762 213 378 468 1886 597 787
Cuts at: 6723 6991 1239
Size: 268 1524
Fragments arranged by size:
1886 1524 787 762 597 468 393 378
268 213
EcoNI CCTnn'n_nnAGG
Cuts at: 2636 3312 2636
Size: 676 6600
EcoO109I rG'GnC_Cy
Cuts at: 2356 2356
Size: 7276
EcoRI G'AATT_C
Cuts at: 2741 3111 3760 3919 4585 4963 2741
Size: 370 649 159 666 378 5054
Fragments arranged by size:
5054 666 649 378 370 159
EcoRV GAT'ATC
Cuts at: 3964 3964
Size: 7276
FspI TGC'GCA
Cuts at: 5782 5782
Size: 7276
HaeII r_GCGC'y
Cuts at: 1678 3324 6423 1678
Size: 1646 3099 2531
Fragments arranged by size:
3099 2531 1646
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2034 2066 2157 2189 3735
Size: 32 135 32 462 32 91 32 1546
Cuts at: 3735 3767 3953 3985 5917 5949 5991 6023 1373
Size: 32 186 32 1932 32 42 32 2626
Fragments arranged by size:
2626 1932 1546 462 186 135 91 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4620 4866 669
Size: 988 281 2682 246 3079
Fragments arranged by size:
3079 2682 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2562 748
Size: 1814 5462
HpaI GTT'AAC
Cuts at: 4866 4866
Size: 7276
KpnI G_GTAC'C
Cuts at: 4601 4601
Size: 7276
MfeI C'AATT_G
Cuts at: 4875 4875
Size: 7276
MluI A'CGCG_T
Cuts at: 7152 7152
Size: 7276
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2007 2184 3500 3577 6390 6574 1305
Size: 702 177 1316 77 2813 184 2007
Fragments arranged by size:
2813 2007 1316 702 184 177 77
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5454
Fragments arranged by size:
5454 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2114 2604 5234 5593 5752 518
Size: 648 948 490 2630 359 159 2042
Fragments arranged by size:
2630 2042 948 648 490 359 159
MspA1I CmG'CkG
Cuts at: 1606 2554 3167 3926 4692 5369 6517 6762 1606
Size: 948 613 759 766 677 1148 245 2120
Fragments arranged by size:
2120 1148 948 766 759 677 613 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6724
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7276
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7276
NotI GC'GGCC_GC
Cuts at: 4723 4723
Size: 7276
NsiI A_TGCA'T
Cuts at: 3085 3438 3085
Size: 353 6923
NspI r_CATG'y
Cuts at: 1132 1229 2938 3373 3440 4634 6179 1132
Size: 97 1709 435 67 1194 1545 2229
Fragments arranged by size:
2229 1709 1545 1194 435 97 67
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7276
PciI A'CATG_T
Cuts at: 6175 6175
Size: 7276
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7276
PmeI GTTT'AAAC
Cuts at: 4580 4580
Size: 7276
PmlI CAC'GTG
Cuts at: 2430 2430
Size: 7276
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3309 3341 5345 5377 6858 6890 3309
Size: 32 2004 32 1481 32 3695
Fragments arranged by size:
3695 2004 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2356 2356
Size: 7276
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7276
PsiI TTA'TAA
Cuts at: 3837 3878 3950 4002 4846 3837
Size: 41 72 52 844 6267
Fragments arranged by size:
6267 844 72 52 41
PstI C_TGCA'G
Cuts at: 830 4628 830
Size: 3798 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7276
PvuII CAG'CTG
Cuts at: 2554 3167 3926 2554
Size: 613 759 5904
Fragments arranged by size:
5904 759 613
SacI G_AGCT'C
Cuts at: 2127 2127
Size: 7276
SalI G'TCGA_C
Cuts at: 1936 4618 1936
Size: 2682 4594
SbfI CC_TGCA'GG
Cuts at: 4628 4628
Size: 7276
ScaI AGT'ACT
Cuts at: 5524 7236 5524
Size: 1712 5564
SfcI C'TryA_G
Cuts at: 826 1045 2994 3171 4392 4624 5759 6440 6631
Size: 219 1949 177 1221 232 1135 681 191
Cuts at: 6631 826
Size: 1471
Fragments arranged by size:
1949 1471 1221 1135 681 232 219 191
177
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7276
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7276
SmaI CCC'GGG
Cuts at: 1872 4603 1872
Size: 2731 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2126 3582 5380 6281 6543 820
Size: 197 926 183 1456 1798 901 262 1553
Fragments arranged by size:
1798 1553 1456 926 901 262 197 183
SnaBI TAC'GTA
Cuts at: 493 4573 5067 493
Size: 4080 494 2702
Fragments arranged by size:
4080 2702 494
SpeI A'CTAG_T
Cuts at: 152 2405 152
Size: 2253 5023
SphI G_CATG'C
Cuts at: 1132 4634 1132
Size: 3502 3774
SspI AAT'ATT
Cuts at: 5 52 5200 5
Size: 47 5148 2081
Fragments arranged by size:
5148 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7276
StyI C'CwwG_G
Cuts at: 513 1065 2517 3852 4146 4473 4715 513
Size: 552 1452 1335 294 327 242 3074
Fragments arranged by size:
3074 1452 1335 552 327 294 242
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2100 5303 5320 5473 5658 6103 325
Size: 1255 520 3203 17 153 185 445 1498
Fragments arranged by size:
3203 1498 1255 520 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2832 5522 7234
Size: 274 80 33 51 1443 855 2690 1712
Cuts at: 7234 96
Size: 138
Fragments arranged by size:
2690 1712 1443 855 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2090 2122 3607 3639 7236 7268 2090
Size: 32 1485 32 3597 32 2098
Fragments arranged by size:
3597 2098 1485 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4557 1316
Size: 3241 4035
XbaI T'CTAG_A
Cuts at: 4612 4612
Size: 7276
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4480 4480
Size: 7276
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7276
XmaI C'CCGG_G
Cuts at: 1870 4601 1870
Size: 2731 4545
XmnI GAAnn'nnTTC
Cuts at: 4026 4308 5405 4026
Size: 282 1097 5897
Fragments arranged by size:
5897 1097 282
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5084 276
Size: 53 83 186 936 3550 2468
Fragments arranged by size:
3550 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BstZ17I Bsu36I DraIII FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI