Restriction map for
pFN21ASDA1363
(Circular) (Six-base) MAPSORT of: pFN21ASDA1363.seq Check: 6083 from: 1 to: 5953
pFN21ASDA1363 5953 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2205 2205
Size: 5953
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3763 278
Size: 53 83 186 936 2227 2468
Fragments arranged by size:
2468 2227 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2079 3296 1937
Size: 142 1217 4594
Fragments arranged by size:
4594 1217 142
Acc65I G'GTAC_C
Cuts at: 3274 3274
Size: 5953
AclI AA'CG_TT
Cuts at: 1563 4080 4453 1563
Size: 2517 373 3063
Fragments arranged by size:
3063 2517 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2552 4016 5400 5668 1632
Size: 920 1464 1384 268 1917
Fragments arranged by size:
1917 1464 1384 920 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5756
AflIII A'CryG_T
Cuts at: 1203 3053 4852 5829 1203
Size: 1850 1799 977 1327
Fragments arranged by size:
1850 1799 1327 977
AgeI A'CCGG_T
Cuts at: 4784 4784
Size: 5953
AhdI GACnn_n'nnGTC
Cuts at: 4682 4682
Size: 5953
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5953
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5953
AlwNI CAG_nnn'CTG
Cuts at: 2980 3696 5268 2980
Size: 716 1572 3665
Fragments arranged by size:
3665 1572 716
ApaI G_GGCC'C
Cuts at: 2875 2875
Size: 5953
ApaLI G'TGCA_C
Cuts at: 2148 4010 5166 2148
Size: 1862 1156 2935
Fragments arranged by size:
2935 1862 1156
ApoI r'AATT_y
Cuts at: 1493 2209 2496 2574 3262 3488 3640 5749 1493
Size: 716 287 78 688 226 152 2109 1697
Fragments arranged by size:
2109 1697 716 688 287 226 152 78
AseI AT'TA_AT
Cuts at: 160 1032 4507 160
Size: 872 3475 1606
Fragments arranged by size:
3475 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5953
AvaI C'yCGr_G
Cuts at: 1870 1943 3278 1870
Size: 73 1335 4545
Fragments arranged by size:
4545 1335 73
BamHI G'GATC_C
Cuts at: 3283 3650 3283
Size: 367 5586
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3274 4729 618
Size: 325 217 627 1487 1455 1842
Fragments arranged by size:
1842 1487 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2875 3272 1391
Size: 1484 397 4072
Fragments arranged by size:
4072 1484 397
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2241 928
Size: 971 342 4640
Fragments arranged by size:
4640 971 342
BbvCI CC'TCA_GC
Cuts at: 2427 2427
Size: 5953
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4144 4178 1903
Size: 34 2207 34 3678
Fragments arranged by size:
3678 2207 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3845 5055 3845
Size: 1210 4743
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5953
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2057 4564 136
Size: 107 122 71 631 605 385 2507 1525
Fragments arranged by size:
2507 1525 631 605 385 122 107 71
BglII A'GATC_T
Cuts at: 5948 5948
Size: 5953
BlpI GC'TnA_GC
Cuts at: 3370 3370
Size: 5953
Bme1580I G_kGCm'C
Cuts at: 1790 2152 2875 4014 5170 1790
Size: 362 723 1139 1156 2573
Fragments arranged by size:
2573 1156 1139 723 362
BmgBI CAC'GTC
Cuts at: 1318 3054 1318
Size: 1736 4217
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3684 4642 442
Size: 416 53 83 399 24 2267 958 1753
Fragments arranged by size:
2267 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5953
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4613 1239
Size: 3374 2579
Bpu10I CC'TnA_GC
Cuts at: 2427 2490 2427
Size: 63 5890
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2622 4078 4943 5241 2622
Size: 1456 865 298 3334
Fragments arranged by size:
3334 1456 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4616 882
Size: 617 3117 2219
Fragments arranged by size:
3117 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3250 3744 493
Size: 711 2046 494 2702
Fragments arranged by size:
2702 2046 711 494
BsaBI GATnn'nnATC
Cuts at: 5947 5947
Size: 5953
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2133 3760 4142 4777
Size: 53 83 186 936 600 1627 382 635
Cuts at: 4777 275
Size: 1451
Fragments arranged by size:
1627 1451 936 635 600 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3204 4386 4784 5058 5205 5616 3204
Size: 1182 398 274 147 411 3541
Fragments arranged by size:
3541 1182 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5728
Fragments arranged by size:
5728 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2379 3020 1186
Size: 1193 641 4119
Fragments arranged by size:
4119 1193 641
BseYI C'CCAG_C
Cuts at: 2370 5156 5652 2370
Size: 2786 496 2671
Fragments arranged by size:
2786 2671 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2831 1163
Size: 573 1095 4285
Fragments arranged by size:
4285 1095 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3403 4164 5192 1540
Size: 275 186 1402 761 1028 2301
Fragments arranged by size:
2301 1402 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2152 4014 4099 5170 2152
Size: 1862 85 1071 2935
Fragments arranged by size:
2935 1862 1071 85
BsmI GAATG_Cn'
Cuts at: 2443 3464 3557 2443
Size: 1021 93 4839
Fragments arranged by size:
4839 1021 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3069 3069
Size: 5953
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2152 2875 3272 4014 4099 5170 1391
Size: 399 362 723 397 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 723 399 397 362 85
BspEI T'CCGG_A
Cuts at: 3204 5616 3204
Size: 2412 3541
BspHI T'CATG_A
Cuts at: 2500 3842 5572 2500
Size: 1342 1730 2881
Fragments arranged by size:
2881 1730 1342
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2205 3308 844
Size: 1361 1103 3489
Fragments arranged by size:
3489 1361 1103
BsrBI CCG'CTC
Cuts at: 1394 1823 2991 3840 1394
Size: 429 1168 849 3507
Fragments arranged by size:
3507 1168 849 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2427 2823 2953 3003 4448 4622 65
Size: 1183 1179 396 130 50 1445 174 1396
Fragments arranged by size:
1445 1396 1183 1179 396 174 130 50
BsrFI r'CCGG_y
Cuts at: 1700 1810 3113 4597 4784 1700
Size: 110 1303 1484 187 2869
Fragments arranged by size:
2869 1484 1303 187 110
BsrGI T'GTAC_A
Cuts at: 96 2414 2932 96
Size: 2318 518 3117
Fragments arranged by size:
3117 2318 518
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5953
BssSI C'ACGA_G
Cuts at: 4013 5025 4013
Size: 1012 4941
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2577 794
Size: 1783 4170
BstBI TT'CG_AA
Cuts at: 2007 4757 2007
Size: 2750 3203
BstEII G'GTnAC_C
Cuts at: 1184 2266 3124 1184
Size: 1082 858 4013
Fragments arranged by size:
4013 1082 858
BstXI CCAn_nnnn'nTGG
Cuts at: 3103 3103
Size: 5953
BstYI r'GATC_y
Cuts at: 1972 3283 3650 4035 4052 5504 5590 5602 5948
Size: 1311 367 385 17 1452 86 12 346
Cuts at: 5948 1972
Size: 1977
Fragments arranged by size:
1977 1452 1311 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2359 513
Size: 552 1294 4107
Fragments arranged by size:
4107 1294 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5214
Fragments arranged by size:
5214 683 56
BtsI GCAGTG_nn'
Cuts at: 3024 3469 4263 4283 3024
Size: 445 794 20 4694
Fragments arranged by size:
4694 794 445 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5953
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5918
DraI TTT'AAA
Cuts at: 3257 3257
Size: 5953
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4960 809
Size: 507 3644 1802
Fragments arranged by size:
3644 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2064 3400 4289
Size: 54 1425 50 275 18 234 1336 889
Cuts at: 4289 5918 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1336 889 275 234 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3400 1537
Size: 275 1588 4090
Fragments arranged by size:
4090 1588 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2322 2847 3883 1353
Size: 969 525 1036 3423
Fragments arranged by size:
3423 1036 969 525
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2983 4540 4914 5060 387
Size: 1064 138 1394 1557 374 146 1280
Fragments arranged by size:
1557 1394 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2552 4016 4613 5400 5668 1239
Size: 393 920 1464 597 787 268 1524
Fragments arranged by size:
1524 1464 920 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2312 2871 2312
Size: 559 5394
EcoRI G'AATT_C
Cuts at: 3262 3640 3262
Size: 378 5575
FspI TGC'GCA
Cuts at: 4459 4459
Size: 5953
HaeII r_GCGC'y
Cuts at: 1678 2063 5100 1678
Size: 385 3037 2531
Fragments arranged by size:
3037 2531 385
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2975 3007 3118 3150 4594
Size: 32 135 32 1403 32 111 32 1444
Cuts at: 4594 4626 4668 4700 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1444 1403 135 111 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2827 3297 3543 669
Size: 988 281 889 470 246 3079
Fragments arranged by size:
3079 988 889 470 281 246
HindIII A'AGCT_T
Cuts at: 748 2673 2697 748
Size: 1925 24 4004
Fragments arranged by size:
4004 1925 24
HpaI GTT'AAC
Cuts at: 2827 3543 2827
Size: 716 5237
KpnI G_GTAC'C
Cuts at: 3278 3278
Size: 5953
MfeI C'AATT_G
Cuts at: 3552 3552
Size: 5953
MluI A'CGCG_T
Cuts at: 5829 5829
Size: 5953
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2130 5067 5251 1305
Size: 825 2937 184 2007
Fragments arranged by size:
2937 2007 825 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2066 10
Size: 54 1425 343 234 3897
Fragments arranged by size:
3897 1425 343 234 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3911 4270 4429 518
Size: 648 2745 359 159 2042
Fragments arranged by size:
2745 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2089 3369 4046 5194 5439 1606
Size: 483 1280 677 1148 245 2120
Fragments arranged by size:
2120 1280 1148 677 483 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5401
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5953
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5953
NotI GC'GGCC_GC
Cuts at: 3400 3400
Size: 5953
NspI r_CATG'y
Cuts at: 1132 1229 3311 4856 1132
Size: 97 2082 1545 2229
Fragments arranged by size:
2229 2082 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5953
PciI A'CATG_T
Cuts at: 4852 4852
Size: 5953
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2179 1108
Size: 1071 4882
PfoI T'CCnGG_A
Cuts at: 2961 2961
Size: 5953
PmeI GTTT'AAAC
Cuts at: 3257 3257
Size: 5953
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4022 4054 5535 5567 4022
Size: 32 1481 32 4408
Fragments arranged by size:
4408 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5953
PsiI TTA'TAA
Cuts at: 3523 3523
Size: 5953
PspOMI G'GGCC_C
Cuts at: 2871 2871
Size: 5953
PstI C_TGCA'G
Cuts at: 830 3027 3305 830
Size: 2197 278 3478
Fragments arranged by size:
3478 2197 278
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5953
SalI G'TCGA_C
Cuts at: 1936 3295 1936
Size: 1359 4594
SapI GCTCTTCn'nnn_
Cuts at: 2322 2322
Size: 5953
SbfI CC_TGCA'GG
Cuts at: 3305 3305
Size: 5953
ScaI AGT'ACT
Cuts at: 4201 5913 4201
Size: 1712 4241
SfcI C'TryA_G
Cuts at: 826 1045 3023 3301 4436 5117 5308 826
Size: 219 1978 278 1135 681 191 1471
Fragments arranged by size:
1978 1471 1135 681 278 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5953
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5953
SmaI CCC'GGG
Cuts at: 1872 3280 1872
Size: 1408 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2637 4057 4958 5220 820
Size: 197 926 694 1420 901 262 1553
Fragments arranged by size:
1553 1420 926 901 694 262 197
SnaBI TAC'GTA
Cuts at: 493 3250 3744 493
Size: 2757 494 2702
Fragments arranged by size:
2757 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5953
SphI G_CATG'C
Cuts at: 1132 3311 1132
Size: 2179 3774
SspI AAT'ATT
Cuts at: 5 52 3877 5
Size: 47 3825 2081
Fragments arranged by size:
3825 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5953
StyI C'CwwG_G
Cuts at: 513 1065 2376 2620 3392 513
Size: 552 1311 244 772 3074
Fragments arranged by size:
3074 1311 772 552 244
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2285 2767 3980 3997 4150 4335 4780
Size: 1255 705 482 1213 17 153 185 445
Cuts at: 4780 325
Size: 1498
Fragments arranged by size:
1498 1255 1213 705 482 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2414 2932 4199
Size: 274 80 33 51 1443 437 518 1267
Cuts at: 4199 5911 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1267 518 437 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5913 5945 5913
Size: 32 5921
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5953
XbaI T'CTAG_A
Cuts at: 3289 3289
Size: 5953
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3193 3193
Size: 5953
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5953
XmaI C'CCGG_G
Cuts at: 1870 3278 1870
Size: 1408 4545
XmnI GAAnn'nnTTC
Cuts at: 4082 4082
Size: 5953
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3761 276
Size: 53 83 186 936 2227 2468
Fragments arranged by size:
2468 2227 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI