Restriction map for
pFN21ASDA1348
(Circular) (Six-base) MAPSORT of: pFN21ASDA1348.seq Check: 2964 from: 1 to: 6316
pFN21ASDA1348 6316 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4126 278
Size: 53 83 186 936 2590 2468
Fragments arranged by size:
2590 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2092 3659 1937
Size: 155 1567 4594
Fragments arranged by size:
4594 1567 155
Acc65I G'GTAC_C
Cuts at: 3637 3637
Size: 6316
AclI AA'CG_TT
Cuts at: 1563 4443 4816 1563
Size: 2880 373 3063
Fragments arranged by size:
3063 2880 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3101 4379 5763 6031 1632
Size: 1469 1278 1384 268 1917
Fragments arranged by size:
1917 1469 1384 1278 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6119
AflIII A'CryG_T
Cuts at: 1203 2939 5215 6192 1203
Size: 1736 2276 977 1327
Fragments arranged by size:
2276 1736 1327 977
AgeI A'CCGG_T
Cuts at: 5147 5147
Size: 6316
AhdI GACnn_n'nnGTC
Cuts at: 5045 5045
Size: 6316
AleI CACnn'nnGTG
Cuts at: 1166 2630 2649 3563 1166
Size: 1464 19 914 3919
Fragments arranged by size:
3919 1464 914 19
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2845 1184
Size: 1661 4655
AlwNI CAG_nnn'CTG
Cuts at: 2530 2668 2734 3382 4059 5631 2530
Size: 138 66 648 677 1572 3215
Fragments arranged by size:
3215 1572 677 648 138 66
ApaLI G'TGCA_C
Cuts at: 4373 5529 4373
Size: 1156 5160
ApoI r'AATT_y
Cuts at: 1493 2053 2332 2850 3625 3851 4003 6112 1493
Size: 560 279 518 775 226 152 2109 1697
Fragments arranged by size:
2109 1697 775 560 518 279 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2714 4870 160
Size: 872 1682 2156 1606
Fragments arranged by size:
2156 1682 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6316
AvaI C'yCGr_G
Cuts at: 1870 1943 2151 3641 1870
Size: 73 208 1490 4545
Fragments arranged by size:
4545 1490 208 73
BamHI G'GATC_C
Cuts at: 3646 4013 3646
Size: 367 5949
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2155 3309 3637 5092 618
Size: 325 217 627 368 1154 328 1455 1842
Fragments arranged by size:
1842 1455 1154 627 368 328 325 217
BanII G_rGCy'C
Cuts at: 1391 3372 3411 3635 1391
Size: 1981 39 224 4072
Fragments arranged by size:
4072 1981 224 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5345
BbvCI CC'TCA_GC
Cuts at: 3221 3318 3221
Size: 97 6219
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4507 4541 1903
Size: 34 2570 34 3678
Fragments arranged by size:
3678 2570 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4208 5418 4208
Size: 1210 5106
BclI T'GATC_A
Cuts at: 1548 3444 1548
Size: 1896 4420
BfrBI ATG'CAT
Cuts at: 2462 2462
Size: 6316
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4927 136
Size: 107 122 71 631 605 3255 1525
Fragments arranged by size:
3255 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6311 6311
Size: 6316
BlpI GC'TnA_GC
Cuts at: 3195 3275 3733 3195
Size: 80 458 5778
Fragments arranged by size:
5778 458 80
Bme1580I G_kGCm'C
Cuts at: 1790 2470 3312 4377 5533 1790
Size: 680 842 1065 1156 2573
Fragments arranged by size:
2573 1156 1065 842 680
BmgBI CAC'GTC
Cuts at: 1318 3289 1318
Size: 1971 4345
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2372 3047 4047
Size: 416 53 83 399 24 955 675 1000
Cuts at: 4047 5005 442
Size: 958 1753
Fragments arranged by size:
1753 1000 958 955 675 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6316
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2089 2552 2786 3134 4976 1239
Size: 850 463 234 348 1842 2579
Fragments arranged by size:
2579 1842 850 463 348 234
Bpu10I CC'TnA_GC
Cuts at: 2259 3221 3318 2259
Size: 962 97 5257
Fragments arranged by size:
5257 962 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2576 4441 5306 5604 2576
Size: 1865 865 298 3288
Fragments arranged by size:
3288 1865 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3346 4979 882
Size: 617 1847 1633 2219
Fragments arranged by size:
2219 1847 1633 617
BsaAI yAC'GTr
Cuts at: 493 1204 2863 2942 3613 4107 493
Size: 711 1659 79 671 494 2702
Fragments arranged by size:
2702 1659 711 671 494 79
BsaBI GATnn'nnATC
Cuts at: 6310 6310
Size: 6316
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3042 4123 4505 5140
Size: 53 83 186 936 1509 1081 382 635
Cuts at: 5140 275
Size: 1451
Fragments arranged by size:
1509 1451 1081 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4749 5147 5421 5568 5979 4749
Size: 398 274 147 411 5086
Fragments arranged by size:
5086 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6091
Fragments arranged by size:
6091 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6316
BseYI C'CCAG_C
Cuts at: 5519 6015 5519
Size: 496 5820
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5743
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3766 4527 5555 1540
Size: 275 186 1765 761 1028 2301
Fragments arranged by size:
2301 1765 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2538 2994 4377 4462 5533 2538
Size: 456 1383 85 1071 3321
Fragments arranged by size:
3321 1383 1071 456 85
BsmI GAATG_Cn'
Cuts at: 3108 3827 3920 3108
Size: 719 93 5504
Fragments arranged by size:
5504 719 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2470 2538 2994 3312 3372 3411 3635
Size: 399 680 68 456 318 60 39 224
Cuts at: 3635 4377 4462 5533 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 680 456 399 318 224
85 68 60 39
BspEI T'CCGG_A
Cuts at: 5979 5979
Size: 6316
BspHI T'CATG_A
Cuts at: 4205 5935 4205
Size: 1730 4586
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3671 844
Size: 2827 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3493 4203 1394
Size: 429 1670 710 3507
Fragments arranged by size:
3507 1670 710 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2070 2078 3286 3512 4811 4985 65
Size: 1183 822 8 1208 226 1299 174 1396
Fragments arranged by size:
1396 1299 1208 1183 822 226 174 8
BsrFI r'CCGG_y
Cuts at: 1700 1810 2388 4960 5147 1700
Size: 110 578 2572 187 2869
Fragments arranged by size:
2869 2572 578 187 110
BsrGI T'GTAC_A
Cuts at: 96 3537 96
Size: 3441 2875
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6316
BssSI C'ACGA_G
Cuts at: 3413 4376 5388 3413
Size: 963 1012 4341
Fragments arranged by size:
4341 1012 963
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2668 3382 794
Size: 1874 714 3728
Fragments arranged by size:
3728 1874 714
BstBI TT'CG_AA
Cuts at: 2007 5120 2007
Size: 3113 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6316
BstXI CCAn_nnnn'nTGG
Cuts at: 2846 2846
Size: 6316
BstYI r'GATC_y
Cuts at: 1972 2046 3646 4013 4398 4415 5867 5953 5965
Size: 74 1600 367 385 17 1452 86 12
Cuts at: 5965 6311 1972
Size: 346 1977
Fragments arranged by size:
1977 1600 1452 385 367 346 86 74
17 12
Bsu36I CC'TnA_GG
Cuts at: 3201 3201
Size: 6316
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5764
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3518 487
Size: 683 56 2292 3285
Fragments arranged by size:
3285 2292 683 56
BtsI GCAGTG_nn'
Cuts at: 2481 2511 3832 4626 4646 2481
Size: 30 1321 794 20 4151
Fragments arranged by size:
4151 1321 794 30 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6316
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6281
DraI TTT'AAA
Cuts at: 3620 3620
Size: 6316
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2454 5323 809
Size: 507 1138 2869 1802
Fragments arranged by size:
2869 1802 1138 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3763 4652 6281
Size: 54 1425 50 275 18 1933 889 1629
Cuts at: 6281 8
Size: 43
Fragments arranged by size:
1933 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3763 1537
Size: 275 1951 4090
Fragments arranged by size:
4090 1951 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2495 4246 1353
Size: 1142 1751 3423
Fragments arranged by size:
3423 1751 1142
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4903 5277 5423 387
Size: 1064 138 3314 374 146 1280
Fragments arranged by size:
3314 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2089 2552 2786 3101 3134 4379 4976
Size: 393 457 463 234 315 33 1245 597
Cuts at: 4976 5763 6031 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1245 787 597 463 457 393 315
268 234 33
EcoNI CCTnn'n_nnAGG
Cuts at: 3317 3317
Size: 6316
EcoO109I rG'GnC_Cy
Cuts at: 2764 3060 3136 3225 2764
Size: 296 76 89 5855
Fragments arranged by size:
5855 296 89 76
EcoRI G'AATT_C
Cuts at: 2850 3625 4003 2850
Size: 775 378 5163
Fragments arranged by size:
5163 775 378
EcoRV GAT'ATC
Cuts at: 3550 3550
Size: 6316
FspI TGC'GCA
Cuts at: 4822 4822
Size: 6316
HaeII r_GCGC'y
Cuts at: 1678 5463 1678
Size: 3785 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2612 2644 3441 3473 4957
Size: 32 135 32 1040 32 797 32 1484
Cuts at: 4957 4989 5031 5063 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1484 1040 797 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3660 3906 669
Size: 988 281 1722 246 3079
Fragments arranged by size:
3079 1722 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6316
HpaI GTT'AAC
Cuts at: 3906 3906
Size: 6316
KpnI G_GTAC'C
Cuts at: 3641 3641
Size: 6316
MfeI C'AATT_G
Cuts at: 3915 3915
Size: 6316
MluI A'CGCG_T
Cuts at: 6192 6192
Size: 6316
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5430 5614 1305
Size: 4125 184 2007
Fragments arranged by size:
4125 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4494
Fragments arranged by size:
4494 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2463 2630 2649 2687 2844 3563 4274
Size: 648 1297 167 19 38 157 719 711
Cuts at: 4274 4633 4792 518
Size: 359 159 2042
Fragments arranged by size:
2042 1297 719 711 648 359 167 159
157 38 19
MspA1I CmG'CkG
Cuts at: 1606 2362 3079 3405 3732 4409 5557 5802 1606
Size: 756 717 326 327 677 1148 245 2120
Fragments arranged by size:
2120 1148 756 717 677 327 326 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5764
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6316
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6316
NotI GC'GGCC_GC
Cuts at: 3763 3763
Size: 6316
NsiI A_TGCA'T
Cuts at: 2464 2464
Size: 6316
NspI r_CATG'y
Cuts at: 1132 1229 2466 2920 3274 3455 3674 5219 1132
Size: 97 1237 454 354 181 219 1545 2229
Fragments arranged by size:
2229 1545 1237 454 354 219 181 97
PasI CC'CwG_GG
Cuts at: 1348 2600 1348
Size: 1252 5064
PciI A'CATG_T
Cuts at: 5215 5215
Size: 6316
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2186 2530 2734 1108
Size: 1078 344 204 4690
Fragments arranged by size:
4690 1078 344 204
PmeI GTTT'AAAC
Cuts at: 3620 3620
Size: 6316
PmlI CAC'GTG
Cuts at: 2863 2942 2863
Size: 79 6237
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4385 4417 5898 5930 4385
Size: 32 1481 32 4771
Fragments arranged by size:
4771 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6316
PsiI TTA'TAA
Cuts at: 3886 3886
Size: 6316
PstI C_TGCA'G
Cuts at: 830 3059 3393 3668 830
Size: 2229 334 275 3478
Fragments arranged by size:
3478 2229 334 275
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6316
PvuII CAG'CTG
Cuts at: 2362 3079 2362
Size: 717 5599
SalI G'TCGA_C
Cuts at: 1936 3658 1936
Size: 1722 4594
SapI GCTCTTCn'nnn_
Cuts at: 2495 2495
Size: 6316
SbfI CC_TGCA'GG
Cuts at: 3059 3668 3059
Size: 609 5707
ScaI AGT'ACT
Cuts at: 2032 4564 6276 2032
Size: 2532 1712 2072
Fragments arranged by size:
2532 2072 1712
SfcI C'TryA_G
Cuts at: 826 1045 2209 3055 3389 3664 4799 5480 5671
Size: 219 1164 846 334 275 1135 681 191
Cuts at: 5671 826
Size: 1471
Fragments arranged by size:
1471 1164 1135 846 681 334 275 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6316
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6316
SmaI CCC'GGG
Cuts at: 1872 2153 3643 1872
Size: 281 1490 4545
Fragments arranged by size:
4545 1490 281
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2591 4420 5321 5583 820
Size: 197 926 648 1829 901 262 1553
Fragments arranged by size:
1829 1553 926 901 648 262 197
SnaBI TAC'GTA
Cuts at: 493 3613 4107 493
Size: 3120 494 2702
Fragments arranged by size:
3120 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6316
SphI G_CATG'C
Cuts at: 1132 3674 1132
Size: 2542 3774
SspI AAT'ATT
Cuts at: 5 52 4240 5
Size: 47 4188 2081
Fragments arranged by size:
4188 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6316
StyI C'CwwG_G
Cuts at: 513 1065 2893 3755 513
Size: 552 1828 862 3074
Fragments arranged by size:
3074 1828 862 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2176 2613 4343 4360 4513 4698 5143
Size: 1255 596 437 1730 17 153 185 445
Cuts at: 5143 325
Size: 1498
Fragments arranged by size:
1730 1498 1255 596 445 437 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2030 2718 3537
Size: 274 80 33 51 1443 53 688 819
Cuts at: 3537 4562 6274 96
Size: 1025 1712 138
Fragments arranged by size:
1712 1443 1025 819 688 274 138 80
53 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6276 6308 6276
Size: 32 6284
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6316
XbaI T'CTAG_A
Cuts at: 3652 3652
Size: 6316
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2377 2909 2377
Size: 532 5784
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6316
XmaI C'CCGG_G
Cuts at: 1870 2151 3641 1870
Size: 281 1490 4545
Fragments arranged by size:
4545 1490 281
XmnI GAAnn'nnTTC
Cuts at: 2277 4445 2277
Size: 2168 4148
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4124 276
Size: 53 83 186 936 2590 2468
Fragments arranged by size:
2590 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI