Restriction map for
pFN21ASDA1341
(Circular) (Six-base) MAPSORT of: pFN21ASDA1341.seq Check: 5539 from: 1 to: 6529
pFN21ASDA1341 6529 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2088 2088
Size: 6529
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4339 278
Size: 53 83 186 936 2803 2468
Fragments arranged by size:
2803 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2710 3322 3872 1937
Size: 773 612 550 4594
Fragments arranged by size:
4594 773 612 550
Acc65I G'GTAC_C
Cuts at: 3850 3850
Size: 6529
AclI AA'CG_TT
Cuts at: 1563 4656 5029 1563
Size: 3093 373 3063
Fragments arranged by size:
3093 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2703 4592 5976 6244 1632
Size: 1071 1889 1384 268 1917
Fragments arranged by size:
1917 1889 1384 1071 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6332
AflIII A'CryG_T
Cuts at: 1203 2611 5428 6405 1203
Size: 1408 2817 977 1327
Fragments arranged by size:
2817 1408 1327 977
AgeI A'CCGG_T
Cuts at: 5360 5360
Size: 6529
AhdI GACnn_n'nnGTC
Cuts at: 5258 5258
Size: 6529
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6529
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6529
AlwNI CAG_nnn'CTG
Cuts at: 4272 5844 4272
Size: 1572 4957
ApaLI G'TGCA_C
Cuts at: 4586 5742 4586
Size: 1156 5373
ApoI r'AATT_y
Cuts at: 1493 2284 2462 2477 2641 3094 3647 3838 4064
Size: 791 178 15 164 453 553 191 226
Cuts at: 4064 4216 6325 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 791 553 453 226 191 178
164 152 15
AseI AT'TA_AT
Cuts at: 160 1032 5083 160
Size: 872 4051 1606
Fragments arranged by size:
4051 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6529
AvaI C'yCGr_G
Cuts at: 1870 1943 2051 3854 1870
Size: 73 108 1803 4545
Fragments arranged by size:
4545 1803 108 73
BamHI G'GATC_C
Cuts at: 2548 3859 4226 2548
Size: 1311 367 4851
Fragments arranged by size:
4851 1311 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2047 3850 5305 618
Size: 325 217 627 260 1803 1455 1842
Fragments arranged by size:
1842 1803 1455 627 325 260 217
BanII G_rGCy'C
Cuts at: 1391 2418 3848 1391
Size: 1027 1430 4072
Fragments arranged by size:
4072 1430 1027
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2533 2826 928
Size: 971 634 293 4631
Fragments arranged by size:
4631 971 634 293
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4720 4754 1903
Size: 34 2783 34 3678
Fragments arranged by size:
3678 2783 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4421 5631 4421
Size: 1210 5319
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6529
BfrBI ATG'CAT
Cuts at: 2932 3821 2932
Size: 889 5640
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2039 5140 136
Size: 107 122 71 631 605 367 3101 1525
Fragments arranged by size:
3101 1525 631 605 367 122 107 71
BglII A'GATC_T
Cuts at: 2374 6524 2374
Size: 4150 2379
BlpI GC'TnA_GC
Cuts at: 3946 3946
Size: 6529
Bme1580I G_kGCm'C
Cuts at: 1790 2052 2294 2852 4590 5746 1790
Size: 262 242 558 1738 1156 2573
Fragments arranged by size:
2573 1738 1156 558 262 242
BmgBI CAC'GTC
Cuts at: 1318 2614 3278 1318
Size: 1296 664 4569
Fragments arranged by size:
4569 1296 664
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4260 5218 442
Size: 416 53 83 399 24 2843 958 1753
Fragments arranged by size:
2843 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6529
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2316 3453 5189 1239
Size: 1077 1137 1736 2579
Fragments arranged by size:
2579 1736 1137 1077
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4654 5519 5817 4654
Size: 865 298 5366
Fragments arranged by size:
5366 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5192 882
Size: 617 3693 2219
Fragments arranged by size:
3693 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2410 3008 3826 4320 493
Size: 711 1206 598 818 494 2702
Fragments arranged by size:
2702 1206 818 711 598 494
BsaBI GATnn'nnATC
Cuts at: 3525 3538 6523 3525
Size: 13 2985 3531
Fragments arranged by size:
3531 2985 13
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2031 4336 4718 5353
Size: 53 83 186 936 498 2305 382 635
Cuts at: 5353 275
Size: 1451
Fragments arranged by size:
2305 1451 936 635 498 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3456 4962 5360 5634 5781 6192 3456
Size: 1506 398 274 147 411 3793
Fragments arranged by size:
3793 1506 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2598 2628 1178
Size: 30 165 30 1195 30 5079
Fragments arranged by size:
5079 1195 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3171 3225 1186
Size: 1985 54 4490
Fragments arranged by size:
4490 1985 54
BseYI C'CCAG_C
Cuts at: 5732 6228 5732
Size: 496 6033
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5956
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3979 4740 5768 1540
Size: 275 186 1978 761 1028 2301
Fragments arranged by size:
2301 1978 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2418 4590 4675 5746 2418
Size: 2172 85 1071 3201
Fragments arranged by size:
3201 2172 1071 85
BsmI GAATG_Cn'
Cuts at: 4040 4133 4040
Size: 93 6436
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2294 2418 2852 3848 4590 4675
Size: 399 262 242 124 434 996 742 85
Cuts at: 4675 5746 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 996 742 434 399 262 242
124 85
BspEI T'CCGG_A
Cuts at: 6192 6192
Size: 6529
BspHI T'CATG_A
Cuts at: 2962 4418 6148 2962
Size: 1456 1730 3343
Fragments arranged by size:
3343 1730 1456
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2088 3884 844
Size: 1244 1796 3489
Fragments arranged by size:
3489 1796 1244
BsrBI CCG'CTC
Cuts at: 1394 1823 4416 1394
Size: 429 2593 3507
Fragments arranged by size:
3507 2593 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2576 3192 3342 5024 5198 65
Size: 1183 1328 616 150 1682 174 1396
Fragments arranged by size:
1682 1396 1328 1183 616 174 150
BsrFI r'CCGG_y
Cuts at: 1700 1810 2705 5173 5360 1700
Size: 110 895 2468 187 2869
Fragments arranged by size:
2869 2468 895 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6529
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6529
BssSI C'ACGA_G
Cuts at: 4589 5601 4589
Size: 1012 5517
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6529
BstBI TT'CG_AA
Cuts at: 2007 5333 2007
Size: 3326 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6529
BstYI r'GATC_y
Cuts at: 1972 2374 2548 3365 3389 3859 4226 4611 4628
Size: 402 174 817 24 470 367 385 17
Cuts at: 4628 6080 6166 6178 6524 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 817 470 402 385 367 346
174 86 24 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2108 2127 3238 513
Size: 552 957 86 19 1111 3804
Fragments arranged by size:
3804 1111 957 552 86 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3260 487
Size: 683 56 2034 3756
Fragments arranged by size:
3756 2034 683 56
BtsI GCAGTG_nn'
Cuts at: 3337 4045 4839 4859 3337
Size: 708 794 20 5007
Fragments arranged by size:
5007 794 708 20
ClaI AT'CG_AT
Cuts at: 1554 3519 1554
Size: 1965 4564
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2710 2745 529
Size: 35 2146 35 4313
Fragments arranged by size:
4313 2146 35 35
DraI TTT'AAA
Cuts at: 3833 3833
Size: 6529
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5536 809
Size: 507 4220 1802
Fragments arranged by size:
4220 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3217 3976 4865
Size: 54 1425 50 275 18 1387 759 889
Cuts at: 4865 6494 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1387 889 759 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3976 1537
Size: 275 2164 4090
Fragments arranged by size:
4090 2164 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2202 2961 4459 1353
Size: 849 759 1498 3423
Fragments arranged by size:
3423 1498 849 759
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2042 2150 5116 5490 5636 387
Size: 1064 138 453 108 2966 374 146 1280
Fragments arranged by size:
2966 1280 1064 453 374 146 138 108
EcoICRI GAG'CTC
Cuts at: 2416 2416
Size: 6529
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2316 2703 3453 4592 5189 5976 6244
Size: 393 684 387 750 1139 597 787 268
Cuts at: 6244 1239
Size: 1524
Fragments arranged by size:
1524 1139 787 750 684 597 393 387
268
EcoRI G'AATT_C
Cuts at: 2284 2462 3838 4216 2284
Size: 178 1376 378 4597
Fragments arranged by size:
4597 1376 378 178
FspI TGC'GCA
Cuts at: 5035 5035
Size: 6529
HaeII r_GCGC'y
Cuts at: 1678 5676 1678
Size: 3998 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2598 2612 2630 2644 2936
Size: 32 135 32 1026 14 18 14 292
Cuts at: 2936 2968 5170 5202 5244 5276 1373
Size: 32 2202 32 42 32 2626
Fragments arranged by size:
2626 2202 1026 292 135 42 32 32
32 32 32 18 14 14
HincII GTy'rAC
Cuts at: 669 1657 1938 3873 4119 669
Size: 988 281 1935 246 3079
Fragments arranged by size:
3079 1935 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6529
HpaI GTT'AAC
Cuts at: 4119 4119
Size: 6529
KpnI G_GTAC'C
Cuts at: 3854 3854
Size: 6529
MfeI C'AATT_G
Cuts at: 2871 4128 2871
Size: 1257 5272
MluI A'CGCG_T
Cuts at: 6405 6405
Size: 6529
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2738 5643 5827 1305
Size: 1433 2905 184 2007
Fragments arranged by size:
2905 2007 1433 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4707
Fragments arranged by size:
4707 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3026 3521 4487 4846 5005 518
Size: 648 1860 495 966 359 159 2042
Fragments arranged by size:
2042 1860 966 648 495 359 159
MspA1I CmG'CkG
Cuts at: 1606 2110 2129 3746 3945 4622 5770 6015 1606
Size: 504 19 1617 199 677 1148 245 2120
Fragments arranged by size:
2120 1617 1148 677 504 245 199 19
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5020
Fragments arranged by size:
5020 957 552
NdeI CA'TA_TG
Cuts at: 387 2347 387
Size: 1960 4569
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6529
NotI GC'GGCC_GC
Cuts at: 3976 3976
Size: 6529
NsiI A_TGCA'T
Cuts at: 2934 3823 2934
Size: 889 5640
NspI r_CATG'y
Cuts at: 1132 1229 2936 3887 5432 1132
Size: 97 1707 951 1545 2229
Fragments arranged by size:
2229 1707 1545 951 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6529
PciI A'CATG_T
Cuts at: 5428 5428
Size: 6529
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3134 1108
Size: 2026 4503
PfoI T'CCnGG_A
Cuts at: 2602 2602
Size: 6529
PmeI GTTT'AAAC
Cuts at: 3833 3833
Size: 6529
PmlI CAC'GTG
Cuts at: 3008 3008
Size: 6529
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4598 4630 6111 6143 4598
Size: 32 1481 32 4984
Fragments arranged by size:
4984 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6529
PsiI TTA'TAA
Cuts at: 4099 4099
Size: 6529
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2701 2733 2701
Size: 32 6497
PstI C_TGCA'G
Cuts at: 830 2085 3761 3881 830
Size: 1255 1676 120 3478
Fragments arranged by size:
3478 1676 1255 120
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6529
PvuII CAG'CTG
Cuts at: 3746 3746
Size: 6529
SacI G_AGCT'C
Cuts at: 2418 2418
Size: 6529
SacII CC_GC'GG
Cuts at: 2111 2130 2111
Size: 19 6510
SalI G'TCGA_C
Cuts at: 1936 3871 1936
Size: 1935 4594
SbfI CC_TGCA'GG
Cuts at: 3881 3881
Size: 6529
ScaI AGT'ACT
Cuts at: 4777 6489 4777
Size: 1712 4817
SexAI A'CCwGG_T
Cuts at: 3487 3487
Size: 6529
SfcI C'TryA_G
Cuts at: 826 1045 2081 2235 3757 3877 5012 5693 5884
Size: 219 1036 154 1522 120 1135 681 191
Cuts at: 5884 826
Size: 1471
Fragments arranged by size:
1522 1471 1135 1036 681 219 191 154
120
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6529
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6529
SmaI CCC'GGG
Cuts at: 1872 2053 3856 1872
Size: 181 1803 4545
Fragments arranged by size:
4545 1803 181
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4633 5534 5796 820
Size: 197 926 2690 901 262 1553
Fragments arranged by size:
2690 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3826 4320 493
Size: 3333 494 2702
Fragments arranged by size:
3333 2702 494
SpeI A'CTAG_T
Cuts at: 152 3251 152
Size: 3099 3430
SphI G_CATG'C
Cuts at: 1132 3887 1132
Size: 2755 3774
SspI AAT'ATT
Cuts at: 5 52 2539 4453 5
Size: 47 2487 1914 2081
Fragments arranged by size:
2487 2081 1914 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6529
StyI C'CwwG_G
Cuts at: 513 1065 2022 3968 513
Size: 552 957 1946 3074
Fragments arranged by size:
3074 1946 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4556 4573 4726 4911 5356 325
Size: 1255 2976 17 153 185 445 1498
Fragments arranged by size:
2976 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4775 6487 96
Size: 274 80 33 51 1443 2798 1712 138
Fragments arranged by size:
2798 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6489 6521 6489
Size: 32 6497
Tth111I GACn'n_nGTC
Cuts at: 1316 3036 1316
Size: 1720 4809
XbaI T'CTAG_A
Cuts at: 3865 3865
Size: 6529
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6529
XmaI C'CCGG_G
Cuts at: 1870 2051 3854 1870
Size: 181 1803 4545
Fragments arranged by size:
4545 1803 181
XmnI GAAnn'nnTTC
Cuts at: 2880 4658 2880
Size: 1778 4751
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4337 276
Size: 53 83 186 936 2803 2468
Fragments arranged by size:
2803 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PsrI
PstI PvuI PvuII SacI SacII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PpuMI PspOMI PspXI RsrII
SanDI SapI SfiI SfoI SrfI SwaI XcmI