Restriction map for
pFN21ASDA1337
(Circular) (Six-base) MAPSORT of: pFN21ASDA1337.seq Check: 80 from: 1 to: 8905
pFN21ASDA1337 8905 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5086 5086
Size: 8905
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4433 6715 278
Size: 53 83 186 936 2897 2282 2468
Fragments arranged by size:
2897 2468 2282 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3332 3453 4554 4688 5136 5396 6248 1937
Size: 1395 121 1101 134 448 260 852 4594
Fragments arranged by size:
4594 1395 1101 852 448 260 134 121
Acc65I G'GTAC_C
Cuts at: 2715 6226 2715
Size: 3511 5394
AclI AA'CG_TT
Cuts at: 1563 7032 7405 1563
Size: 5469 373 3063
Fragments arranged by size:
5469 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2450 3212 4765 5232 6968 8352 8620 1632
Size: 818 762 1553 467 1736 1384 268 1917
Fragments arranged by size:
1917 1736 1553 1384 818 762 467 268
AflII C'TTAA_G
Cuts at: 820 1017 2399 820
Size: 197 1382 7326
Fragments arranged by size:
7326 1382 197
AflIII A'CryG_T
Cuts at: 1203 3960 4080 7804 8781 1203
Size: 2757 120 3724 977 1327
Fragments arranged by size:
3724 2757 1327 977 120
AgeI A'CCGG_T
Cuts at: 7736 7736
Size: 8905
AhdI GACnn_n'nnGTC
Cuts at: 7634 7634
Size: 8905
AleI CACnn'nnGTG
Cuts at: 1166 3308 6002 1166
Size: 2142 2694 4069
Fragments arranged by size:
4069 2694 2142
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4748 5318 6056 1184
Size: 3564 570 738 4033
Fragments arranged by size:
4033 3564 738 570
AlwNI CAG_nnn'CTG
Cuts at: 2497 2698 3103 3435 4168 4327 5242 5473 6648
Size: 201 405 332 733 159 915 231 1175
Cuts at: 6648 8220 2497
Size: 1572 3182
Fragments arranged by size:
3182 1572 1175 915 733 405 332 231
201 159
ApaI G_GGCC'C
Cuts at: 2116 2130 4007 4291 5236 6127 2116
Size: 14 1877 284 945 891 4894
Fragments arranged by size:
4894 1877 945 891 284 14
ApaLI G'TGCA_C
Cuts at: 6962 8118 6962
Size: 1156 7749
ApoI r'AATT_y
Cuts at: 1493 6214 6440 6592 8701 1493
Size: 4721 226 152 2109 1697
Fragments arranged by size:
4721 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7459 160
Size: 872 6427 1606
Fragments arranged by size:
6427 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8905
AvaI C'yCGr_G
Cuts at: 1870 1943 2529 3974 4103 5122 5306 5323 5486
Size: 73 586 1445 129 1019 184 17 163
Cuts at: 5486 6230 1870
Size: 744 4545
Fragments arranged by size:
4545 1445 1019 744 586 184 163 129
73 17
BamHI G'GATC_C
Cuts at: 2414 6235 6602 2414
Size: 3821 367 4717
Fragments arranged by size:
4717 3821 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2715 2777 3883 4508 4621
Size: 325 217 627 928 62 1106 625 113
Cuts at: 4621 4876 5172 6055 6104 6137 6226 7681 618
Size: 255 296 883 49 33 89 1455 1842
Fragments arranged by size:
1842 1455 1106 928 883 627 625 325
296 255 217 113 89 62 49 33
BanII G_rGCy'C
Cuts at: 1391 2116 2130 2536 2853 3008 3122 3200 3899
Size: 725 14 406 317 155 114 78 699
Cuts at: 3899 4007 4291 4718 5236 5313 5495 6127 6224
Size: 108 284 427 518 77 182 632 97
Cuts at: 6224 1391
Size: 4072
Fragments arranged by size:
4072 725 699 632 518 427 406 317
284 182 155 114 108 97 78 77
14
BbeI G_GCGC'C
Cuts at: 2781 6059 2781
Size: 3278 5627
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2685 3672 3754 3941 5056 928
Size: 971 786 987 82 187 1115 4777
Fragments arranged by size:
4777 1115 987 971 786 187 82
BbvCI CC'TCA_GC
Cuts at: 5660 5660
Size: 8905
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5179 5213 7096 7130 1903
Size: 34 3242 34 1883 34 3678
Fragments arranged by size:
3678 3242 1883 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3566 5032 6797 8007 3566
Size: 1466 1765 1210 4464
Fragments arranged by size:
4464 1765 1466 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8905
BfrBI ATG'CAT
Cuts at: 4872 4872
Size: 8905
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3320 4286 5615
Size: 107 122 71 631 605 1648 966 1329
Cuts at: 5615 7516 136
Size: 1901 1525
Fragments arranged by size:
1901 1648 1525 1329 966 631 605 122
107 71
BglII A'GATC_T
Cuts at: 3075 8900 3075
Size: 5825 3080
BlpI GC'TnA_GC
Cuts at: 2699 4895 6322 2699
Size: 2196 1427 5282
Fragments arranged by size:
5282 2196 1427
Bme1580I G_kGCm'C
Cuts at: 1790 2116 2130 2770 4007 4291 4626 4956 5175
Size: 326 14 640 1237 284 335 330 219
Cuts at: 5175 5236 5914 5940 6127 6966 8122 1790
Size: 61 678 26 187 839 1156 2573
Fragments arranged by size:
2573 1237 1156 839 678 640 335 330
326 284 219 187 61 26 14
BmgBI CAC'GTC
Cuts at: 1318 3961 4081 5110 1318
Size: 2643 120 1029 5113
Fragments arranged by size:
5113 2643 1029 120
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2401 4189 4831
Size: 416 53 83 399 24 984 1788 642
Cuts at: 4831 5230 6636 7594 442
Size: 399 1406 958 1753
Fragments arranged by size:
1788 1753 1406 984 958 642 416 399
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4476 1056
Size: 3420 5485
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3193 4381 4642 3193
Size: 1188 261 7456
Fragments arranged by size:
7456 1188 261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2224 2672 3146 3889 3911 4061 4088 4457
Size: 985 448 474 743 22 150 27 369
Cuts at: 4457 4688 4885 5447 5725 5829 5890 5891 7565
Size: 231 197 562 278 104 61 1 1674
Cuts at: 7565 1239
Size: 2579
Fragments arranged by size:
2579 1674 985 743 562 474 448 369
278 231 197 150 104 61 27 22
1
Bpu10I CC'TnA_GC
Cuts at: 4349 4456 5660 4349
Size: 107 1204 7594
Fragments arranged by size:
7594 1204 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2972 4322 6077 7030 7895 8193 2972
Size: 1350 1755 953 865 298 3684
Fragments arranged by size:
3684 1755 1350 953 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2633 3515 5112 7568 882
Size: 617 1134 882 1597 2456 2219
Fragments arranged by size:
2456 2219 1597 1134 882 617
BsaAI yAC'GTr
Cuts at: 493 1204 3310 3544 4792 5403 5887 6202 6696
Size: 711 2106 234 1248 611 484 315 494
Cuts at: 6696 493
Size: 2702
Fragments arranged by size:
2702 2106 1248 711 611 494 484 315
234
BsaBI GATnn'nnATC
Cuts at: 8899 8899
Size: 8905
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2778 4430 5079 5352
Size: 53 83 186 936 1245 1652 649 273
Cuts at: 5352 5841 6056 6712 7094 7729 275
Size: 489 215 656 382 635 1451
Fragments arranged by size:
1652 1451 1245 936 656 649 635 489
382 273 215 186 83 53
BsaWI w'CCGG_w
Cuts at: 4425 7338 7736 8010 8157 8568 4425
Size: 2913 398 274 147 411 4762
Fragments arranged by size:
4762 2913 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2438 2468 3749 3779 4547
Size: 30 165 30 1035 30 1281 30 768
Cuts at: 4547 4577 5246 5276 1178
Size: 30 669 30 4807
Fragments arranged by size:
4807 1281 1035 768 669 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2081 2084 2093 5254 5648 5752 1186
Size: 895 3 9 3161 394 104 4339
Fragments arranged by size:
4339 3161 895 394 104 9 3
BseYI C'CCAG_C
Cuts at: 2188 2217 2243 2723 2787 3001 3029 3264 3775
Size: 29 26 480 64 214 28 235 511
Cuts at: 3775 4072 4209 5448 5712 6043 6059 6133 8108
Size: 297 137 1239 264 331 16 74 1975
Cuts at: 8108 8604 2188
Size: 496 2489
Fragments arranged by size:
2489 1975 1239 511 496 480 331 297
264 235 214 137 74 64 29 28
26 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3929 4157 4175 5390 5582 1163
Size: 573 2193 228 18 1215 192 4486
Fragments arranged by size:
4486 2193 1215 573 228 192 18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6355 7116 8144 1540
Size: 275 186 4354 761 1028 2301
Fragments arranged by size:
4354 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2256 2853 2988 3200 3232 3899 5529 5658 6153
Size: 597 135 212 32 667 1630 129 495
Cuts at: 6153 6966 7051 8122 2256
Size: 813 85 1071 3039
Fragments arranged by size:
3039 1630 1071 813 667 597 495 212
135 129 85 32
BsiWI C'GTAC_G
Cuts at: 5864 5864
Size: 8905
BsmI GAATG_Cn'
Cuts at: 6416 6509 6416
Size: 93 8812
BsmBI CGTCTCn'nnnn_
Cuts at: 2453 3951 4071 4237 4970 5908 2453
Size: 1498 120 166 733 938 5450
Fragments arranged by size:
5450 1498 938 733 166 120
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2116 2130 2256 2536 2770 2853 2988
Size: 399 326 14 126 280 234 83 135
Cuts at: 2988 3008 3122 3200 3232 3899 4007 4291 4626
Size: 20 114 78 32 667 108 284 335
Cuts at: 4626 4718 4956 5175 5236 5313 5495 5529 5658
Size: 92 238 219 61 77 182 34 129
Cuts at: 5658 5914 5940 6127 6153 6224 6966 7051 8122
Size: 256 26 187 26 71 742 85 1071
Cuts at: 8122 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 667 399 335 326 284
280 256 238 234 219 187 182 135
129 126 114 108 92 85 83 78
77 71 61 34 32 26 26 20
14
BspEI T'CCGG_A
Cuts at: 4425 8568 4425
Size: 4143 4762
BspHI T'CATG_A
Cuts at: 3039 6794 8524 3039
Size: 3755 1730 3420
Fragments arranged by size:
3755 3420 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3970 5086 5325 5451 5528 5808 5826 6260
Size: 3126 1116 239 126 77 280 18 434
Cuts at: 6260 844
Size: 3489
Fragments arranged by size:
3489 3126 1116 434 280 239 126 77
18
BsrBI CCG'CTC
Cuts at: 1394 1823 2514 2622 5709 6162 6792 1394
Size: 429 691 108 3087 453 630 3507
Fragments arranged by size:
3507 3087 691 630 453 429 108
BsrDI GCAATG_nn'
Cuts at: 65 1248 3438 7400 7574 65
Size: 1183 2190 3962 174 1396
Fragments arranged by size:
3962 2190 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2430 4266 4476 5017 7549 7736 1700
Size: 110 620 1836 210 541 2532 187 2869
Fragments arranged by size:
2869 2532 1836 620 541 210 187 110
BsrGI T'GTAC_A
Cuts at: 96 4515 96
Size: 4419 4486
BssHII G'CGCG_C
Cuts at: 1925 2800 2864 1925
Size: 875 64 7966
Fragments arranged by size:
7966 875 64
BssSI C'ACGA_G
Cuts at: 2360 2978 6146 6965 7977 2360
Size: 618 3168 819 1012 3288
Fragments arranged by size:
3288 3168 1012 819 618
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2497 3435 4168 4368 5281 5473 5482 5679
Size: 1703 938 733 200 913 192 9 197
Cuts at: 5679 794
Size: 4020
Fragments arranged by size:
4020 1703 938 913 733 200 197 192
9
BstBI TT'CG_AA
Cuts at: 2007 7709 2007
Size: 5702 3203
BstEII G'GTnAC_C
Cuts at: 1184 3301 5689 1184
Size: 2117 2388 4400
Fragments arranged by size:
4400 2388 2117
BstXI CCAn_nnnn'nTGG
Cuts at: 3661 3791 5114 3661
Size: 130 1323 7452
Fragments arranged by size:
7452 1323 130
BstYI r'GATC_y
Cuts at: 1972 2349 2414 2656 2992 3075 3216 3771 5376
Size: 377 65 242 336 83 141 555 1605
Cuts at: 5376 6235 6602 6987 7004 8456 8542 8554 8900
Size: 859 367 385 17 1452 86 12 346
Cuts at: 8900 1972
Size: 1977
Fragments arranged by size:
1977 1605 1452 859 555 385 377 367
346 336 242 141 86 83 65 17
12
Bsu36I CC'TnA_GG
Cuts at: 4482 4482
Size: 8905
BtgI C'CryG_G
Cuts at: 513 1065 2022 2277 2550 2592 4138 4858 5049
Size: 552 957 255 273 42 1546 720 191
Cuts at: 5049 5338 5382 5610 5766 5919 513
Size: 289 44 228 156 153 3499
Fragments arranged by size:
3499 1546 957 720 552 289 273 255
228 191 156 153 44 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5182 5911 5941 487
Size: 683 56 3956 729 30 3451
Fragments arranged by size:
3956 3451 729 683 56 30
BtsI GCAGTG_nn'
Cuts at: 3217 5352 5378 6421 7215 7235 3217
Size: 2135 26 1043 794 20 4887
Fragments arranged by size:
4887 2135 1043 794 26 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8905
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3905 3940 529
Size: 35 3341 35 5494
Fragments arranged by size:
5494 3341 35 35
DraI TTT'AAA
Cuts at: 6209 6209
Size: 8905
DraIII CAC_nnn'GTG
Cuts at: 2983 2983
Size: 8905
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5108 7912 809
Size: 507 3792 2804 1802
Fragments arranged by size:
3792 2804 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2442 2751 3132
Size: 54 1425 50 275 18 612 309 381
Cuts at: 3132 3312 3321 3585 3831 4141 4794 5027 5607
Size: 180 9 264 246 310 653 233 580
Cuts at: 5607 5616 5895 6352 7241 8870 8
Size: 9 279 457 889 1629 43
Fragments arranged by size:
1629 1425 889 653 612 580 457 381
310 309 279 275 264 246 233 180
54 50 43 18 9 9
EagI C'GGCC_G
Cuts at: 1537 1812 6352 1537
Size: 275 4540 4090
Fragments arranged by size:
4540 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2261 3188 3536 4047 4304 4439 4526 5162
Size: 908 927 348 511 257 135 87 636
Cuts at: 5162 5948 6835 1353
Size: 786 887 3423
Fragments arranged by size:
3423 927 908 887 786 636 511 348
257 135 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2440 3122 4262 7492 7866 8012
Size: 1064 138 851 682 1140 3230 374 146
Cuts at: 8012 387
Size: 1280
Fragments arranged by size:
3230 1280 1140 1064 851 682 374 146
138
EcoICRI GAG'CTC
Cuts at: 2851 3198 3897 2851
Size: 347 699 7859
Fragments arranged by size:
7859 699 347
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2224 2450 2672 3146 3212 3889 3911
Size: 393 592 226 222 474 66 677 22
Cuts at: 3911 4061 4088 4457 4688 4765 4885 5232 5447
Size: 150 27 369 231 77 120 347 215
Cuts at: 5447 5725 5829 5890 5891 6968 7565 8352 8620
Size: 278 104 61 1 1077 597 787 268
Cuts at: 8620 1239
Size: 1524
Fragments arranged by size:
1524 1077 787 677 597 592 474 393
369 347 278 268 231 226 222 215
150 120 104 77 66 61 27 22
1
EcoNI CCTnn'n_nnAGG
Cuts at: 2139 4920 5159 5189 5789 2139
Size: 2781 239 30 600 5255
Fragments arranged by size:
5255 2781 600 239 30
EcoO109I rG'GnC_Cy
Cuts at: 2112 2126 2127 2573 3100 3172 4003 4004 4183
Size: 14 1 446 527 72 831 1 179
Cuts at: 4183 4288 4462 5232 6097 6123 6124 6189 2112
Size: 105 174 770 865 26 1 65 4828
Fragments arranged by size:
4828 865 831 770 527 446 179 174
105 72 65 26 14 1 1 1
EcoRI G'AATT_C
Cuts at: 6214 6592 6214
Size: 378 8527
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3733 4795 4846 4870 3733
Size: 1062 51 24 7768
Fragments arranged by size:
7768 1062 51 24
FspI TGC'GCA
Cuts at: 3209 3389 3680 7411 3209
Size: 180 291 3731 4703
Fragments arranged by size:
4703 3731 291 180
HaeII r_GCGC'y
Cuts at: 1678 2399 2473 2781 2905 4356 4649 5571 5857
Size: 721 74 308 124 1451 293 922 286
Cuts at: 5857 6059 8052 1678
Size: 202 1993 2531
Fragments arranged by size:
2531 1993 1451 922 721 308 293 286
202 124 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2438 2470 4046 4078 7546
Size: 32 135 32 866 32 1576 32 3468
Cuts at: 7546 7578 7620 7652 1373
Size: 32 42 32 2626
Fragments arranged by size:
3468 2626 1576 866 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5083 5397 6249 6495 669
Size: 988 281 3145 314 852 246 3079
Fragments arranged by size:
3145 3079 988 852 314 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8905
HpaI GTT'AAC
Cuts at: 6495 6495
Size: 8905
KasI G'GCGC_C
Cuts at: 2777 6055 2777
Size: 3278 5627
KpnI G_GTAC'C
Cuts at: 2719 6230 2719
Size: 3511 5394
MfeI C'AATT_G
Cuts at: 6504 6504
Size: 8905
MluI A'CGCG_T
Cuts at: 8781 8781
Size: 8905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3964 4149 8019 8203 1305
Size: 2659 185 3870 184 2007
Fragments arranged by size:
3870 2659 2007 185 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3134 3314 3323 4796 5897
Size: 54 1425 343 1302 180 9 1473 1101
Cuts at: 5897 10
Size: 3018
Fragments arranged by size:
3018 1473 1425 1302 1101 343 180 54
9
MslI CAynn'nnrTG
Cuts at: 518 1166 2276 3308 3611 4322 5603 6002 6863
Size: 648 1110 1032 303 711 1281 399 861
Cuts at: 6863 7222 7381 518
Size: 359 159 2042
Fragments arranged by size:
2042 1281 1110 1032 861 711 648 399
359 303 159
MspA1I CmG'CkG
Cuts at: 1606 2317 2488 2497 2552 2594 2695 3001 3264
Size: 711 171 9 55 42 101 306 263
Cuts at: 3264 3435 3516 3676 3912 4140 4159 4209 4550
Size: 171 81 160 236 228 19 50 341
Cuts at: 4550 5332 5473 5612 6321 6998 8146 8391 1606
Size: 782 141 139 709 677 1148 245 2120
Fragments arranged by size:
2120 1148 782 711 709 677 341 306
263 245 236 228 171 171 160 141
139 101 81 55 50 42 19 9
NaeI GCC'GGC
Cuts at: 4268 4478 5019 4268
Size: 210 541 8154
Fragments arranged by size:
8154 541 210
NarI GG'CG_CC
Cuts at: 2778 6056 2778
Size: 3278 5627
NcoI C'CATG_G
Cuts at: 513 1065 2022 2277 5049 5382 513
Size: 552 957 255 2772 333 4036
Fragments arranged by size:
4036 2772 957 552 333 255
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8905
NgoMIV G'CCGG_C
Cuts at: 4266 4476 5017 4266
Size: 210 541 8154
Fragments arranged by size:
8154 541 210
NheI G'CTAG_C
Cuts at: 1052 4472 1052
Size: 3420 5485
NotI GC'GGCC_GC
Cuts at: 6352 6352
Size: 8905
NsiI A_TGCA'T
Cuts at: 4874 4874
Size: 8905
NspI r_CATG'y
Cuts at: 1132 1229 3412 6263 7808 1132
Size: 97 2183 2851 1545 2229
Fragments arranged by size:
2851 2229 2183 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2212 4000 4467 6100 6127 1348
Size: 864 1788 467 1633 27 4126
Fragments arranged by size:
4126 1788 1633 864 467 27
PciI A'CATG_T
Cuts at: 7804 7804
Size: 8905
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3103 3565 1108
Size: 1995 462 6448
Fragments arranged by size:
6448 1995 462
PfoI T'CCnGG_A
Cuts at: 2955 4392 2955
Size: 1437 7468
PmeI GTTT'AAAC
Cuts at: 6209 6209
Size: 8905
PmlI CAC'GTG
Cuts at: 3544 5887 3544
Size: 2343 6562
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6974 7006 8487 8519 6974
Size: 32 1481 32 7360
Fragments arranged by size:
7360 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3100 4183 4462 3100
Size: 1083 279 7543
Fragments arranged by size:
7543 1083 279
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8905
PsiI TTA'TAA
Cuts at: 6475 6475
Size: 8905
PspOMI G'GGCC_C
Cuts at: 2112 2126 4003 4287 5232 6123 2112
Size: 14 1877 284 945 891 4894
Fragments arranged by size:
4894 1877 945 891 284 14
PstI C_TGCA'G
Cuts at: 830 2052 2270 2493 3184 3706 3856 4116 4164
Size: 1222 218 223 691 522 150 260 48
Cuts at: 4164 4416 4497 5277 5478 6159 6257 830
Size: 252 81 780 201 681 98 3478
Fragments arranged by size:
3478 1222 780 691 681 522 260 252
223 218 201 150 98 81 48
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8905
PvuII CAG'CTG
Cuts at: 2488 2497 3435 3516 3676 3912 4159 5332 5473
Size: 9 938 81 160 236 247 1173 141
Cuts at: 5473 2488
Size: 5920
Fragments arranged by size:
5920 1173 938 247 236 160 141 81
9
SacI G_AGCT'C
Cuts at: 2853 3200 3899 2853
Size: 347 699 7859
Fragments arranged by size:
7859 699 347
SacII CC_GC'GG
Cuts at: 2553 2595 4141 5613 2553
Size: 42 1546 1472 5845
Fragments arranged by size:
5845 1546 1472 42
SalI G'TCGA_C
Cuts at: 1936 5395 6247 1936
Size: 3459 852 4594
Fragments arranged by size:
4594 3459 852
SanDI GG'GwC_CC
Cuts at: 4462 4462
Size: 8905
SapI GCTCTTCn'nnn_
Cuts at: 2261 3188 4047 2261
Size: 927 859 7119
Fragments arranged by size:
7119 927 859
SbfI CC_TGCA'GG
Cuts at: 4497 6257 4497
Size: 1760 7145
ScaI AGT'ACT
Cuts at: 3563 7153 8865 3563
Size: 3590 1712 3603
Fragments arranged by size:
3603 3590 1712
SexAI A'CCwGG_T
Cuts at: 5784 5784
Size: 8905
SfcI C'TryA_G
Cuts at: 826 1045 2048 2266 2489 3180 3702 3852 4112
Size: 219 1003 218 223 691 522 150 260
Cuts at: 4112 4160 4412 4493 4555 4689 5254 5273 5474
Size: 48 252 81 62 134 565 19 201
Cuts at: 5474 6155 6253 7388 8069 8260 826
Size: 681 98 1135 681 191 1471
Fragments arranged by size:
1471 1135 1003 691 681 681 565 522
260 252 223 219 218 201 191 150
134 98 81 62 48 19
SfiI GGCCn_nnn'nGGCC
Cuts at: 3320 5615 3320
Size: 2295 6610
SfoI GGC'GCC
Cuts at: 2779 6057 2779
Size: 3278 5627
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8905
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8905
SmaI CCC'GGG
Cuts at: 1872 5124 5325 6232 1872
Size: 3252 201 907 4545
Fragments arranged by size:
4545 3252 907 201
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2399 2987 4337 6092 7009 7910
Size: 197 926 456 588 1350 1755 917 901
Cuts at: 7910 8172 820
Size: 262 1553
Fragments arranged by size:
1755 1553 1350 926 917 901 588 456
262 197
SnaBI TAC'GTA
Cuts at: 493 6202 6696 493
Size: 5709 494 2702
Fragments arranged by size:
5709 2702 494
SpeI A'CTAG_T
Cuts at: 152 5032 152
Size: 4880 4025
SphI G_CATG'C
Cuts at: 1132 6263 1132
Size: 5131 3774
SspI AAT'ATT
Cuts at: 5 52 6829 5
Size: 47 6777 2081
Fragments arranged by size:
6777 2081 47
StuI AGG'CCT
Cuts at: 1517 2366 5480 1517
Size: 849 3114 4942
Fragments arranged by size:
4942 3114 849
StyI C'CwwG_G
Cuts at: 513 1065 2022 2277 4589 4743 4800 4956 5049
Size: 552 957 255 2312 154 57 156 93
Cuts at: 5049 5382 6344 513
Size: 333 962 3074
Fragments arranged by size:
3074 2312 962 957 552 333 255 156
154 93 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2961 3671 4201 5645 6092 6125 6932
Size: 1255 1381 710 530 1444 447 33 807
Cuts at: 6932 6949 7102 7287 7732 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1444 1381 1255 807 710 530 447
445 185 153 33 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3561 4341 4515
Size: 274 80 33 51 1443 1584 780 174
Cuts at: 4515 4827 5700 7151 8863 96
Size: 312 873 1451 1712 138
Fragments arranged by size:
1712 1584 1451 1443 873 780 312 274
174 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8865 8897 8865
Size: 32 8873
Tth111I GACn'n_nGTC
Cuts at: 1316 3620 1316
Size: 2304 6601
XbaI T'CTAG_A
Cuts at: 6241 6241
Size: 8905
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2902 3353 3548 4015 5056 5212 2902
Size: 451 195 467 1041 156 6595
Fragments arranged by size:
6595 1041 467 451 195 156
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8905
XmaI C'CCGG_G
Cuts at: 1870 5122 5323 6230 1870
Size: 3252 201 907 4545
Fragments arranged by size:
4545 3252 907 201
XmnI GAAnn'nnTTC
Cuts at: 7034 7034
Size: 8905
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4431 6713 276
Size: 53 83 186 936 2897 2282 2468
Fragments arranged by size:
2897 2468 2282 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BstZ17I EcoRV FseI
FspAI NruI PacI PspXI PsrI RsrII SrfI SwaI