Restriction map for
pFN21ASDA1322
(Circular) (Six-base) MAPSORT of: pFN21ASDA1322.seq Check: 8967 from: 1 to: 6808
pFN21ASDA1322 6808 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4618 278
Size: 53 83 186 936 3082 2468
Fragments arranged by size:
3082 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2086 4151 1937
Size: 149 2065 4594
Fragments arranged by size:
4594 2065 149
Acc65I G'GTAC_C
Cuts at: 4129 4129
Size: 6808
AclI AA'CG_TT
Cuts at: 1563 4935 5308 1563
Size: 3372 373 3063
Fragments arranged by size:
3372 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2087 3027 3378 3911 4871 6255 6523 1632
Size: 455 940 351 533 960 1384 268 1917
Fragments arranged by size:
1917 1384 960 940 533 455 351 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6611
AflIII A'CryG_T
Cuts at: 1203 2116 3486 3837 5707 6684 1203
Size: 913 1370 351 1870 977 1327
Fragments arranged by size:
1870 1370 1327 977 913 351
AgeI A'CCGG_T
Cuts at: 5639 5639
Size: 6808
AhdI GACnn_n'nnGTC
Cuts at: 5537 5537
Size: 6808
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6808
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6808
AlwNI CAG_nnn'CTG
Cuts at: 2110 3946 4042 4551 6123 2110
Size: 1836 96 509 1572 2795
Fragments arranged by size:
2795 1836 1572 509 96
ApaI G_GGCC'C
Cuts at: 2166 2166
Size: 6808
ApaLI G'TGCA_C
Cuts at: 4865 6021 4865
Size: 1156 5652
ApoI r'AATT_y
Cuts at: 1493 3712 4117 4343 4495 6604 1493
Size: 2219 405 226 152 2109 1697
Fragments arranged by size:
2219 2109 1697 405 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5362 160
Size: 872 4330 1606
Fragments arranged by size:
4330 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6808
AvaI C'yCGr_G
Cuts at: 1870 1943 2218 2312 2969 3315 3959 4133 1870
Size: 73 275 94 657 346 644 174 4545
Fragments arranged by size:
4545 657 644 346 275 174 94 73
BamHI G'GATC_C
Cuts at: 4138 4505 4138
Size: 367 6441
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2131 4129 5584 618
Size: 325 217 627 344 1998 1455 1842
Fragments arranged by size:
1998 1842 1455 627 344 325 217
BanII G_rGCy'C
Cuts at: 1391 2166 2642 3297 4036 4127 1391
Size: 775 476 655 739 91 4072
Fragments arranged by size:
4072 775 739 655 476 91
BbeI G_GCGC'C
Cuts at: 2135 2135
Size: 6808
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2196 2880 2893 928
Size: 971 297 684 13 4843
Fragments arranged by size:
4843 971 684 297 13
BbvCI CC'TCA_GC
Cuts at: 2691 2691
Size: 6808
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3991 4025 4999 5033 1903
Size: 34 2054 34 974 34 3678
Fragments arranged by size:
3678 2054 974 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4700 5910 4700
Size: 1210 5598
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6808
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5419 136
Size: 107 122 71 631 605 3747 1525
Fragments arranged by size:
3747 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3162 6803 3162
Size: 3641 3167
BlpI GC'TnA_GC
Cuts at: 3259 4225 3259
Size: 966 5842
Bme1580I G_kGCm'C
Cuts at: 1790 2166 4869 6025 1790
Size: 376 2703 1156 2573
Fragments arranged by size:
2703 2573 1156 376
BmgBI CAC'GTC
Cuts at: 1318 2119 3838 1318
Size: 801 1719 4288
Fragments arranged by size:
4288 1719 801
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3184 3934 4539
Size: 416 53 83 399 24 1767 750 605
Cuts at: 4539 5497 442
Size: 958 1753
Fragments arranged by size:
1767 1753 958 750 605 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6808
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2189 2255 2563 2618 3274 3431 3741 5468
Size: 950 66 308 55 656 157 310 1727
Cuts at: 5468 1239
Size: 2579
Fragments arranged by size:
2579 1727 950 656 310 308 157 66
55
Bpu10I CC'TnA_GC
Cuts at: 2691 3341 4035 2691
Size: 650 694 5464
Fragments arranged by size:
5464 694 650
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2111 2656 4933 5798 6096 2111
Size: 545 2277 865 298 2823
Fragments arranged by size:
2823 2277 865 545 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5471 882
Size: 617 3972 2219
Fragments arranged by size:
3972 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2278 4105 4599 493
Size: 711 1074 1827 494 2702
Fragments arranged by size:
2702 1827 1074 711 494
BsaBI GATnn'nnATC
Cuts at: 6802 6802
Size: 6808
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2132 4615 4997 5632
Size: 53 83 186 936 599 2483 382 635
Cuts at: 5632 275
Size: 1451
Fragments arranged by size:
2483 1451 936 635 599 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5241 5639 5913 6060 6471 5241
Size: 398 274 147 411 5578
Fragments arranged by size:
5578 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6583
Fragments arranged by size:
6583 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2296 2708 3992 1186
Size: 1110 412 1284 4002
Fragments arranged by size:
4002 1284 1110 412
BseYI C'CCAG_C
Cuts at: 2992 3081 6011 6507 2992
Size: 89 2930 496 3293
Fragments arranged by size:
3293 2930 496 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6235
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4258 5019 6047 1540
Size: 275 186 2257 761 1028 2301
Fragments arranged by size:
2301 2257 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2642 3147 3194 3297 4869 4954 6025 2642
Size: 505 47 103 1572 85 1071 3425
Fragments arranged by size:
3425 1572 1071 505 103 85 47
BsmI GAATG_Cn'
Cuts at: 2839 4319 4412 2839
Size: 1480 93 5235
Fragments arranged by size:
5235 1480 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2149 2149
Size: 6808
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2166 2642 3147 3194 3297 4036 4127
Size: 399 376 476 505 47 103 739 91
Cuts at: 4127 4869 4954 6025 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 739 505 476 399 376
103 91 85 47
BspEI T'CCGG_A
Cuts at: 6471 6471
Size: 6808
BspHI T'CATG_A
Cuts at: 2683 3481 4697 6427 2683
Size: 798 1216 1730 3064
Fragments arranged by size:
3064 1730 1216 798
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2118 3975 4163 844
Size: 1274 1857 188 3489
Fragments arranged by size:
3489 1857 1274 188
BsrBI CCG'CTC
Cuts at: 1394 1823 2317 2380 4695 1394
Size: 429 494 63 2315 3507
Fragments arranged by size:
3507 2315 494 429 63
BsrDI GCAATG_nn'
Cuts at: 65 1248 2548 4049 5303 5477 65
Size: 1183 1300 1501 1254 174 1396
Fragments arranged by size:
1501 1396 1300 1254 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2193 3522 5452 5639 1700
Size: 110 383 1329 1930 187 2869
Fragments arranged by size:
2869 1930 1329 383 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6808
BssHII G'CGCG_C
Cuts at: 1925 2337 1925
Size: 412 6396
BssSI C'ACGA_G
Cuts at: 2569 3290 4868 5880 2569
Size: 721 1578 1012 3497
Fragments arranged by size:
3497 1578 1012 721
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6808
BstBI TT'CG_AA
Cuts at: 2007 5612 2007
Size: 3605 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6808
BstXI CCAn_nnnn'nTGG
Cuts at: 3470 3470
Size: 6808
BstYI r'GATC_y
Cuts at: 1972 3162 3780 4138 4505 4890 4907 6359 6445
Size: 1190 618 358 367 385 17 1452 86
Cuts at: 6445 6457 6803 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1190 618 385 367 358 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2211 2656 3661 513
Size: 552 1146 445 1005 3660
Fragments arranged by size:
3660 1146 1005 552 445
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2490 3864 3979 487
Size: 683 56 1264 1374 115 3316
Fragments arranged by size:
3316 1374 1264 683 115 56
BtsI GCAGTG_nn'
Cuts at: 3573 4324 5118 5138 3573
Size: 751 794 20 5243
Fragments arranged by size:
5243 794 751 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6808
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6773
DraI TTT'AAA
Cuts at: 4112 4112
Size: 6808
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2518 2564 5815 809
Size: 507 1202 46 3251 1802
Fragments arranged by size:
3251 1802 1202 507 46
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3524 4255 5144
Size: 54 1425 50 275 18 1694 731 889
Cuts at: 5144 6773 8
Size: 1629 43
Fragments arranged by size:
1694 1629 1425 889 731 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4255 1537
Size: 275 2443 4090
Fragments arranged by size:
4090 2443 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3852 4738 1353
Size: 2499 886 3423
Fragments arranged by size:
3423 2499 886
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5395 5769 5915 387
Size: 1064 138 3806 374 146 1280
Fragments arranged by size:
3806 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2640 3295 2640
Size: 655 6153
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2189 2255 2563 2618 3027 3274
Size: 393 455 102 66 308 55 409 247
Cuts at: 3274 3378 3431 3741 3911 4871 5468 6255 6523
Size: 104 53 310 170 960 597 787 268
Cuts at: 6523 1239
Size: 1524
Fragments arranged by size:
1524 960 787 597 455 409 393 310
308 268 247 170 104 102 66 55
53
EcoO109I rG'GnC_Cy
Cuts at: 3964 3964
Size: 6808
EcoRI G'AATT_C
Cuts at: 4117 4495 4117
Size: 378 6430
EcoRV GAT'ATC
Cuts at: 3763 3763
Size: 6808
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3940 3940
Size: 6808
FspI TGC'GCA
Cuts at: 2396 3732 5314 2396
Size: 1336 1582 3890
Fragments arranged by size:
3890 1582 1336
HaeII r_GCGC'y
Cuts at: 1678 2135 2334 2822 3510 5955 1678
Size: 457 199 488 688 2445 2531
Fragments arranged by size:
2531 2445 688 488 457 199
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2468 2500 2914 2946 3102
Size: 32 135 32 896 32 414 32 156
Cuts at: 3102 3134 3234 3266 5449 5481 5523 5555 1373
Size: 32 100 32 2183 32 42 32 2626
Fragments arranged by size:
2626 2183 896 414 156 135 100 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2087 3675 4152 4398 669
Size: 988 281 149 1588 477 246 3079
Fragments arranged by size:
3079 1588 988 477 281 246 149
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6808
HpaI GTT'AAC
Cuts at: 4398 4398
Size: 6808
KasI G'GCGC_C
Cuts at: 2131 2131
Size: 6808
KpnI G_GTAC'C
Cuts at: 4133 4133
Size: 6808
MfeI C'AATT_G
Cuts at: 4407 4407
Size: 6808
MluI A'CGCG_T
Cuts at: 6684 6684
Size: 6808
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2319 2325 4120 5922 6106 1305
Size: 1014 6 1795 1802 184 2007
Fragments arranged by size:
2007 1802 1795 1014 184 6
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4986
Fragments arranged by size:
4986 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3932 4766 5125 5284 518
Size: 648 2766 834 359 159 2042
Fragments arranged by size:
2766 2042 834 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3005 3100 4224 4901 6049 6294 1606
Size: 1399 95 1124 677 1148 245 2120
Fragments arranged by size:
2120 1399 1148 1124 677 245 95
NarI GG'CG_CC
Cuts at: 2132 2132
Size: 6808
NcoI C'CATG_G
Cuts at: 513 1065 3661 513
Size: 552 2596 3660
Fragments arranged by size:
3660 2596 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6808
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6808
NotI GC'GGCC_GC
Cuts at: 4255 4255
Size: 6808
NruI TCG'CGA
Cuts at: 3849 3849
Size: 6808
NspI r_CATG'y
Cuts at: 1132 1229 3490 3553 4166 5711 1132
Size: 97 2261 63 613 1545 2229
Fragments arranged by size:
2261 2229 1545 613 97 63
PasI CC'CwG_GG
Cuts at: 1348 3880 1348
Size: 2532 4276
PciI A'CATG_T
Cuts at: 3486 5707 3486
Size: 2221 4587
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2212 1108
Size: 1104 5704
PmeI GTTT'AAAC
Cuts at: 4112 4112
Size: 6808
PmlI CAC'GTG
Cuts at: 2278 2278
Size: 6808
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3851 3883 4877 4909 6390 6422 3851
Size: 32 994 32 1481 32 4237
Fragments arranged by size:
4237 1481 994 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3964 3964
Size: 6808
PshAI GACnn'nnGTC
Cuts at: 1613 3306 1613
Size: 1693 5115
PsiI TTA'TAA
Cuts at: 4378 4378
Size: 6808
PspOMI G'GGCC_C
Cuts at: 2162 2162
Size: 6808
PspXI vC'TCGA_Gb
Cuts at: 2969 2969
Size: 6808
PstI C_TGCA'G
Cuts at: 830 2267 2495 3595 4160 830
Size: 1437 228 1100 565 3478
Fragments arranged by size:
3478 1437 1100 565 228
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6808
PvuII CAG'CTG
Cuts at: 3005 3100 3005
Size: 95 6713
SacI G_AGCT'C
Cuts at: 2642 3297 2642
Size: 655 6153
SalI G'TCGA_C
Cuts at: 1936 2085 4150 1936
Size: 149 2065 4594
Fragments arranged by size:
4594 2065 149
SbfI CC_TGCA'GG
Cuts at: 4160 4160
Size: 6808
ScaI AGT'ACT
Cuts at: 5056 6768 5056
Size: 1712 5096
SfcI C'TryA_G
Cuts at: 826 1045 2263 2443 2491 3591 4156 5291 5972
Size: 219 1218 180 48 1100 565 1135 681
Cuts at: 5972 6163 826
Size: 191 1471
Fragments arranged by size:
1471 1218 1135 1100 681 565 219 191
180 48
SfoI GGC'GCC
Cuts at: 2133 2133
Size: 6808
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6808
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6808
SmaI CCC'GGG
Cuts at: 1872 4135 1872
Size: 2263 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2126 2635 2969 4912 5813 6075
Size: 197 926 183 509 334 1943 901 262
Cuts at: 6075 820
Size: 1553
Fragments arranged by size:
1943 1553 926 901 509 334 262 197
183
SnaBI TAC'GTA
Cuts at: 493 4105 4599 493
Size: 3612 494 2702
Fragments arranged by size:
3612 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6808
SphI G_CATG'C
Cuts at: 1132 4166 1132
Size: 3034 3774
SspI AAT'ATT
Cuts at: 5 52 2715 4732 5
Size: 47 2663 2017 2081
Fragments arranged by size:
2663 2081 2017 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6808
StyI C'CwwG_G
Cuts at: 513 1065 3321 3661 4247 513
Size: 552 2256 340 586 3074
Fragments arranged by size:
3074 2256 586 552 340
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3302 4835 4852 5005 5190 5635 325
Size: 1255 1722 1533 17 153 185 445 1498
Fragments arranged by size:
1722 1533 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5054 6766 96
Size: 274 80 33 51 1443 3077 1712 138
Fragments arranged by size:
3077 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3126 3158 3170 3202 6768 6800 3126
Size: 32 12 32 3566 32 3134
Fragments arranged by size:
3566 3134 32 32 32 12
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6808
XbaI T'CTAG_A
Cuts at: 3194 3434 4144 3194
Size: 240 710 5858
Fragments arranged by size:
5858 710 240
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2593 2593
Size: 6808
XhoI C'TCGA_G
Cuts at: 1943 2969 1943
Size: 1026 5782
XmaI C'CCGG_G
Cuts at: 1870 4133 1870
Size: 2263 4545
XmnI GAAnn'nnTTC
Cuts at: 3896 4937 3896
Size: 1041 5767
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4616 276
Size: 53 83 186 936 3082 2468
Fragments arranged by size:
3082 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NruI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoNI FseI FspAI NaeI
NgoMIV NsiI PacI PfoI PsrI RsrII SacII SanDI
SapI SexAI SfiI SrfI SwaI