Restriction map for
pFN21ASDA1318
(Circular) (Six-base) MAPSORT of: pFN21ASDA1318.seq Check: 7325 from: 1 to: 8893
pFN21ASDA1318 8893 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6703 278
Size: 53 83 186 936 5167 2468
Fragments arranged by size:
5167 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2110 2672 3162 3296 6236 1937
Size: 173 562 490 134 2940 4594
Fragments arranged by size:
4594 2940 562 490 173 134
Acc65I G'GTAC_C
Cuts at: 6214 6214
Size: 8893
AclI AA'CG_TT
Cuts at: 1563 7020 7393 1563
Size: 5457 373 3063
Fragments arranged by size:
5457 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2166 2403 2838 2994 3072 3681 3954 4797
Size: 534 237 435 156 78 609 273 843
Cuts at: 4797 5915 5979 6956 8340 8608 1632
Size: 1118 64 977 1384 268 1917
Fragments arranged by size:
1917 1384 1118 977 843 609 534 435
273 268 237 156 78 64
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8696
AflIII A'CryG_T
Cuts at: 1203 4671 7792 8769 1203
Size: 3468 3121 977 1327
Fragments arranged by size:
3468 3121 1327 977
AgeI A'CCGG_T
Cuts at: 7724 7724
Size: 8893
AhdI GACnn_n'nnGTC
Cuts at: 2242 7622 2242
Size: 5380 3513
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8893
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5409 1184
Size: 4225 4668
AlwNI CAG_nnn'CTG
Cuts at: 2146 2195 2312 2351 2504 2621 2972 3011 3050
Size: 49 117 39 153 117 351 39 39
Cuts at: 3050 3128 3206 3284 3323 3503 3581 3620 3932
Size: 78 78 78 39 180 78 39 312
Cuts at: 3932 3971 4010 4049 4088 4352 4430 4505 4586
Size: 39 39 39 39 264 78 75 81
Cuts at: 4586 4664 4703 4742 4820 5132 5171 5258 5327
Size: 78 39 39 78 312 39 87 69
Cuts at: 5327 5366 5864 5995 6636 8208 2146
Size: 39 498 131 641 1572 2831
Fragments arranged by size:
2831 1572 641 498 351 312 312 264
180 153 131 117 117 87 81 78
78 78 78 78 78 78 75 69
49 39 39 39 39 39 39 39
39 39 39 39 39 39
ApaLI G'TGCA_C
Cuts at: 4675 6950 8106 4675
Size: 2275 1156 5462
Fragments arranged by size:
5462 2275 1156
ApoI r'AATT_y
Cuts at: 1493 4261 6202 6428 6580 8689 1493
Size: 2768 1941 226 152 2109 1697
Fragments arranged by size:
2768 2109 1941 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2603 4880 7447 160
Size: 872 1571 2277 2567 1606
Fragments arranged by size:
2567 2277 1606 1571 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8893
AvaI C'yCGr_G
Cuts at: 1870 1943 5496 6218 1870
Size: 73 3553 722 4545
Fragments arranged by size:
4545 3553 722 73
BamHI G'GATC_C
Cuts at: 6223 6590 6223
Size: 367 8526
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6214 7669 618
Size: 325 217 627 4427 1455 1842
Fragments arranged by size:
4427 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2085 3277 3535 3652 3979 4120 4423 4618
Size: 694 1192 258 117 327 141 303 195
Cuts at: 4618 4696 4735 4891 5504 6050 6212 1391
Size: 78 39 156 613 546 162 4072
Fragments arranged by size:
4072 1192 694 613 546 327 303 258
195 162 156 141 117 78 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2739 6122 928
Size: 971 840 3383 3699
Fragments arranged by size:
3699 3383 971 840
BbvCI CC'TCA_GC
Cuts at: 4774 4774
Size: 8893
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5128 5162 7084 7118 1903
Size: 34 3191 34 1922 34 3678
Fragments arranged by size:
3678 3191 1922 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6785 7995 6785
Size: 1210 7683
BclI T'GATC_A
Cuts at: 1548 6136 1548
Size: 4588 4305
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7504 136
Size: 107 122 71 631 605 5832 1525
Fragments arranged by size:
5832 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2923 3842 5018 8888 2923
Size: 919 1176 3870 2928
Fragments arranged by size:
3870 2928 1176 919
BlpI GC'TnA_GC
Cuts at: 6310 6310
Size: 8893
Bme1580I G_kGCm'C
Cuts at: 1790 2320 2359 4679 6954 8110 1790
Size: 530 39 2320 2275 1156 2573
Fragments arranged by size:
2573 2320 2275 1156 530 39
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8893
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6624 7582 442
Size: 416 53 83 399 24 5207 958 1753
Fragments arranged by size:
5207 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2612 1056
Size: 1556 7337
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3549 4381 5669 3549
Size: 832 1288 6773
Fragments arranged by size:
6773 1288 832
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2211 2217 2295 2565 2643 2682 2799 2877
Size: 972 6 78 270 78 39 117 78
Cuts at: 2877 2949 3027 3033 3111 3267 3345 3384 3462
Size: 72 78 6 78 156 78 39 78
Cuts at: 3462 3564 3759 3876 3993 4032 4071 4110 4227
Size: 102 195 117 117 39 39 39 117
Cuts at: 4227 4266 4374 4407 4413 4452 4569 4608 4686
Size: 39 108 33 6 39 117 39 78
Cuts at: 4686 4719 4725 4881 4959 4998 5115 5349 7553
Size: 33 6 156 78 39 117 234 2204
Cuts at: 7553 1239
Size: 2579
Fragments arranged by size:
2579 2204 972 270 234 195 156 156
117 117 117 117 117 117 108 102
78 78 78 78 78 78 78 78
78 72 39 39 39 39 39 39
39 39 39 33 33 6 6 6
6
Bpu10I CC'TnA_GC
Cuts at: 4774 4774
Size: 8893
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4104 5968 7018 7883 8181 4104
Size: 1864 1050 865 298 4816
Fragments arranged by size:
4816 1864 1050 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2117 4762 4861 5937 6108 7556 882
Size: 617 618 2645 99 1076 171 1448 2219
Fragments arranged by size:
2645 2219 1448 1076 618 617 171 99
BsaAI yAC'GTr
Cuts at: 493 1204 6190 6684 493
Size: 711 4986 494 2702
Fragments arranged by size:
4986 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 2655 3706 3784 5720 8887 2655
Size: 1051 78 1936 3167 2661
Fragments arranged by size:
3167 2661 1936 1051 78
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6700 7082 7717 275
Size: 53 83 186 936 5167 382 635 1451
Fragments arranged by size:
5167 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5305 7326 7724 7998 8145 8556 5305
Size: 2021 398 274 147 411 5642
Fragments arranged by size:
5642 2021 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8668
Fragments arranged by size:
8668 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3541 5552 6038 6080 1186
Size: 2355 2011 486 42 3999
Fragments arranged by size:
3999 2355 2011 486 42
BseYI C'CCAG_C
Cuts at: 2693 2871 3064 3277 3613 4459 4696 5458 8096
Size: 178 193 213 336 846 237 762 2638
Cuts at: 8096 8592 2693
Size: 496 2994
Fragments arranged by size:
2994 2638 846 762 496 336 237 213
193 178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2232 3204 4821 1163
Size: 573 496 972 1617 5235
Fragments arranged by size:
5235 1617 972 573 496
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6343 7104 8132 1540
Size: 275 186 4342 761 1028 2301
Fragments arranged by size:
4342 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2573 3535 3979 4120 4196 4423 4679 4735 6050
Size: 962 444 141 76 227 256 56 1315
Cuts at: 6050 6072 6155 6954 7039 8110 2573
Size: 22 83 799 85 1071 3356
Fragments arranged by size:
3356 1315 1071 962 799 444 256 227
141 85 83 76 56 22
BsmI GAATG_Cn'
Cuts at: 2149 6404 6497 2149
Size: 4255 93 4545
Fragments arranged by size:
4545 4255 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2085 2320 2359 2573 3277 3535 3652
Size: 399 295 235 39 214 704 258 117
Cuts at: 3652 3979 4120 4196 4423 4618 4679 4696 4735
Size: 327 141 76 227 195 61 17 39
Cuts at: 4735 4891 5504 6050 6072 6155 6212 6954 7039
Size: 156 613 546 22 83 57 742 85
Cuts at: 7039 8110 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 704 613 546 399 327
295 258 235 227 214 195 156 141
117 85 83 76 61 57 39 39
22 17
BspEI T'CCGG_A
Cuts at: 5305 8556 5305
Size: 3251 5642
BspHI T'CATG_A
Cuts at: 6133 6782 8512 6133
Size: 649 1730 6514
Fragments arranged by size:
6514 1730 649
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6248 844
Size: 5404 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2914 6780 1394
Size: 429 1091 3866 3507
Fragments arranged by size:
3866 3507 1091 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2327 2366 2405 2532 2595 2712 2714
Size: 1183 1079 39 39 127 63 117 2
Cuts at: 2714 2751 3063 3258 3299 3453 3555 3672 3750
Size: 37 312 195 41 154 102 117 78
Cuts at: 3750 3945 4062 4560 4991 7388 7562 65
Size: 195 117 498 431 2397 174 1396
Fragments arranged by size:
2397 1396 1183 1079 498 431 312 195
195 174 154 127 117 117 117 102
78 63 41 39 39 37 2
BsrFI r'CCGG_y
Cuts at: 1700 1810 7537 7724 1700
Size: 110 5727 187 2869
Fragments arranged by size:
5727 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2158 2555 5937 96
Size: 2062 397 3382 3052
Fragments arranged by size:
3382 3052 2062 397
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8893
BssSI C'ACGA_G
Cuts at: 6953 7965 6953
Size: 1012 7881
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8893
BstBI TT'CG_AA
Cuts at: 2007 7697 2007
Size: 5690 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8893
BstXI CCAn_nnnn'nTGG
Cuts at: 2894 3776 4205 5845 5878 2894
Size: 882 429 1640 33 5909
Fragments arranged by size:
5909 1640 882 429 33
BstYI r'GATC_y
Cuts at: 1972 2923 3842 4201 4454 4574 5018 5684 5886
Size: 951 919 359 253 120 444 666 202
Cuts at: 5886 6223 6590 6975 6992 8444 8530 8542 8888
Size: 337 367 385 17 1452 86 12 346
Cuts at: 8888 1972
Size: 1977
Fragments arranged by size:
1977 1452 951 919 666 444 385 367
359 346 337 253 202 120 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3697 3814 3892 5251 3697
Size: 117 78 1359 7339
Fragments arranged by size:
7339 1359 117 78
BtgI C'CryG_G
Cuts at: 513 1065 3143 3471 3690 4851 4929 4968 6030
Size: 552 2078 328 219 1161 78 39 1062
Cuts at: 6030 513
Size: 3376
Fragments arranged by size:
3376 2078 1161 1062 552 328 219 78
39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2931 3149 4011 5367 487
Size: 683 56 1705 218 862 1356 4013
Fragments arranged by size:
4013 1705 1356 862 683 218 56
BtsI GCAGTG_nn'
Cuts at: 2042 3947 4367 4833 6409 7203 7223 2042
Size: 1905 420 466 1576 794 20 3712
Fragments arranged by size:
3712 1905 1576 794 466 420 20
ClaI AT'CG_AT
Cuts at: 1554 5138 1554
Size: 3584 5309
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8858
DraI TTT'AAA
Cuts at: 6197 6197
Size: 8893
DraIII CAC_nnn'GTG
Cuts at: 5946 5946
Size: 8893
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7900 809
Size: 507 6584 1802
Fragments arranged by size:
6584 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3429 5064 6340
Size: 54 1425 50 275 18 1599 1635 1276
Cuts at: 6340 7229 8858 8
Size: 889 1629 43
Fragments arranged by size:
1635 1629 1599 1425 1276 889 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6340 1537
Size: 275 4528 4090
Fragments arranged by size:
4528 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5475 5514 5601 5955 6823 1353
Size: 4122 39 87 354 868 3423
Fragments arranged by size:
4122 3423 868 354 87 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3174 7480 7854 8000 387
Size: 1064 138 1585 4306 374 146 1280
Fragments arranged by size:
4306 1585 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3533 3977 4118 4421 4733 6048 3533
Size: 444 141 303 312 1315 6378
Fragments arranged by size:
6378 1315 444 312 303 141
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2166 2211 2217 2295 2403 2565 2643
Size: 393 534 45 6 78 108 162 78
Cuts at: 2643 2682 2799 2838 2877 2949 2994 3027 3033
Size: 39 117 39 39 72 45 33 6
Cuts at: 3033 3072 3111 3267 3345 3384 3462 3564 3681
Size: 39 39 156 78 39 78 102 117
Cuts at: 3681 3759 3876 3954 3993 4032 4071 4110 4227
Size: 78 117 78 39 39 39 39 117
Cuts at: 4227 4266 4374 4407 4413 4452 4569 4608 4686
Size: 39 108 33 6 39 117 39 78
Cuts at: 4686 4719 4725 4797 4881 4959 4998 5115 5349
Size: 33 6 72 84 78 39 117 234
Cuts at: 5349 5915 5979 6956 7553 8340 8608 1239
Size: 566 64 977 597 787 268 1524
Fragments arranged by size:
1524 977 787 597 566 534 393 268
234 162 156 117 117 117 117 117
117 108 108 102 84 78 78 78
78 78 78 78 78 72 72 64
45 45 39 39 39 39 39 39
39 39 39 39 39 39 39 39
33 33 33 6 6 6 6
EcoO109I rG'GnC_Cy
Cuts at: 4362 5247 5255 4362
Size: 885 8 8000
Fragments arranged by size:
8000 885 8
EcoRI G'AATT_C
Cuts at: 4261 6202 6580 4261
Size: 1941 378 6574
Fragments arranged by size:
6574 1941 378
EcoRV GAT'ATC
Cuts at: 2514 2787 2514
Size: 273 8620
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6146 6146
Size: 8893
FspI TGC'GCA
Cuts at: 7399 7399
Size: 8893
HaeII r_GCGC'y
Cuts at: 1678 5075 8040 1678
Size: 3397 2965 2531
Fragments arranged by size:
3397 2965 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2209 2228 2241 2260 2576
Size: 32 135 32 637 19 13 19 316
Cuts at: 2576 2608 2693 2725 2915 2947 3166 3198 3371
Size: 32 85 32 190 32 219 32 173
Cuts at: 3371 3403 3639 3671 3797 3814 3829 3846 3992
Size: 32 236 32 126 17 15 17 146
Cuts at: 3992 4024 4165 4197 4268 4300 4775 4807 5287
Size: 32 141 32 71 32 475 32 480
Cuts at: 5287 5319 5693 5725 7534 7566 7608 7640 1373
Size: 32 374 32 1809 32 42 32 2626
Fragments arranged by size:
2626 1809 637 480 475 374 316 236
219 190 173 146 141 135 126 85
71 42 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 19 19 17 17 15 13
HincII GTy'rAC
Cuts at: 669 1657 1938 3150 3423 5454 6237 6483 669
Size: 988 281 1212 273 2031 783 246 3079
Fragments arranged by size:
3079 2031 1212 988 783 281 273 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8893
HpaI GTT'AAC
Cuts at: 3423 6483 3423
Size: 3060 5833
KpnI G_GTAC'C
Cuts at: 6218 6218
Size: 8893
MfeI C'AATT_G
Cuts at: 3602 6492 3602
Size: 2890 6003
MluI A'CGCG_T
Cuts at: 8769 8769
Size: 8893
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3560 5864 8007 8191 1305
Size: 2255 2304 2143 184 2007
Fragments arranged by size:
2304 2255 2143 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3431 5066 10
Size: 54 1425 343 1599 1635 3837
Fragments arranged by size:
3837 1635 1599 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2154 5186 5876 6075 6851 7210 7369
Size: 648 988 3032 690 199 776 359 159
Cuts at: 7369 518
Size: 2042
Fragments arranged by size:
3032 2042 988 776 690 648 359 199
159
MspA1I CmG'CkG
Cuts at: 1606 2846 4244 5953 6309 6986 8134 8379 1606
Size: 1240 1398 1709 356 677 1148 245 2120
Fragments arranged by size:
2120 1709 1398 1240 1148 677 356 245
NcoI C'CATG_G
Cuts at: 513 1065 3143 3690 4851 4929 4968 6030 513
Size: 552 2078 547 1161 78 39 1062 3376
Fragments arranged by size:
3376 2078 1161 1062 552 547 78 39
NdeI CA'TA_TG
Cuts at: 387 4827 387
Size: 4440 4453
NheI G'CTAG_C
Cuts at: 1052 2608 1052
Size: 1556 7337
NotI GC'GGCC_GC
Cuts at: 6340 6340
Size: 8893
NspI r_CATG'y
Cuts at: 1132 1229 4675 6080 6251 7796 1132
Size: 97 3446 1405 171 1545 2229
Fragments arranged by size:
3446 2229 1545 1405 171 97
PasI CC'CwG_GG
Cuts at: 1348 2441 5689 1348
Size: 1093 3248 4552
Fragments arranged by size:
4552 3248 1093
PciI A'CATG_T
Cuts at: 4671 7792 4671
Size: 3121 5772
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2273 2543 2621 3011 3128 3284 3323 3362
Size: 1165 270 78 390 117 156 39 39
Cuts at: 3362 3542 3620 4430 4466 4703 4742 1108
Size: 180 78 810 36 237 39 5259
Fragments arranged by size:
5259 1165 810 390 270 237 180 156
117 78 78 39 39 39 36
PfoI T'CCnGG_A
Cuts at: 2659 4204 2659
Size: 1545 7348
PmeI GTTT'AAAC
Cuts at: 6197 6197
Size: 8893
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5606 5638 6962 6994 8475 8507 5606
Size: 32 1324 32 1481 32 5992
Fragments arranged by size:
5992 1481 1324 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 4828 5809 1613
Size: 3215 981 4697
Fragments arranged by size:
4697 3215 981
PsiI TTA'TAA
Cuts at: 6463 6463
Size: 8893
PstI C_TGCA'G
Cuts at: 830 2509 3715 6245 830
Size: 1679 1206 2530 3478
Fragments arranged by size:
3478 2530 1679 1206
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8893
PvuII CAG'CTG
Cuts at: 2846 5953 2846
Size: 3107 5786
SacI G_AGCT'C
Cuts at: 3535 3979 4120 4423 4735 6050 3535
Size: 444 141 303 312 1315 6378
Fragments arranged by size:
6378 1315 444 312 303 141
SalI G'TCGA_C
Cuts at: 1936 6235 1936
Size: 4299 4594
SapI GCTCTTCn'nnn_
Cuts at: 5475 5475
Size: 8893
SbfI CC_TGCA'GG
Cuts at: 6245 6245
Size: 8893
ScaI AGT'ACT
Cuts at: 7141 8853 7141
Size: 1712 7181
SexAI A'CCwGG_T
Cuts at: 5837 5837
Size: 8893
SfcI C'TryA_G
Cuts at: 826 1045 2107 2169 2505 2847 3711 6241 7376
Size: 219 1062 62 336 342 864 2530 1135
Cuts at: 7376 8057 8248 826
Size: 681 191 1471
Fragments arranged by size:
2530 1471 1135 1062 864 681 342 336
219 191 62
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8893
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8893
SmaI CCC'GGG
Cuts at: 1872 5498 6220 1872
Size: 3626 722 4545
Fragments arranged by size:
4545 3626 722
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4119 5983 6997 7898 8160 820
Size: 197 926 2176 1864 1014 901 262 1553
Fragments arranged by size:
2176 1864 1553 1014 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6190 6684 493
Size: 5697 494 2702
Fragments arranged by size:
5697 2702 494
SpeI A'CTAG_T
Cuts at: 152 4723 152
Size: 4571 4322
SphI G_CATG'C
Cuts at: 1132 6251 1132
Size: 5119 3774
SspI AAT'ATT
Cuts at: 5 52 6817 5
Size: 47 6765 2081
Fragments arranged by size:
6765 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8893
StyI C'CwwG_G
Cuts at: 513 1065 3143 3690 4851 4929 4968 6030 6142
Size: 552 2078 547 1161 78 39 1062 112
Cuts at: 6142 6332 513
Size: 190 3074
Fragments arranged by size:
3074 2078 1161 1062 552 547 190 112
78 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2900 3119 3572 3898 4211 6920 6937
Size: 1255 1320 219 453 326 313 2709 17
Cuts at: 6937 7090 7275 7720 325
Size: 153 185 445 1498
Fragments arranged by size:
2709 1498 1320 1255 453 445 326 313
219 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2158 2555 5937
Size: 274 80 33 51 1443 181 397 3382
Cuts at: 5937 7139 8851 96
Size: 1202 1712 138
Fragments arranged by size:
3382 1712 1443 1202 397 274 181 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4186 4218 8853 8885 4186
Size: 32 4635 32 4194
Fragments arranged by size:
4635 4194 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4789 1316
Size: 3473 5420
XbaI T'CTAG_A
Cuts at: 2926 3401 4625 5015 5219 6229 2926
Size: 475 1224 390 204 1010 5590
Fragments arranged by size:
5590 1224 1010 475 390 204
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3265 3265
Size: 8893
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8893
XmaI C'CCGG_G
Cuts at: 1870 5496 6218 1870
Size: 3626 722 4545
Fragments arranged by size:
4545 3626 722
XmnI GAAnn'nnTTC
Cuts at: 6129 7022 6129
Size: 893 8000
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6701 276
Size: 53 83 186 936 5167 2468
Fragments arranged by size:
5167 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I EcoNI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI
SrfI SwaI