Restriction map for
pFN21ASDA1317
(Circular) (Six-base) MAPSORT of: pFN21ASDA1317.seq Check: 638 from: 1 to: 6013
pFN21ASDA1317 6013 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2290 2290
Size: 6013
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3823 278
Size: 53 83 186 936 2287 2468
Fragments arranged by size:
2468 2287 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3356 1937
Size: 1419 4594
Acc65I G'GTAC_C
Cuts at: 3334 3334
Size: 6013
AclI AA'CG_TT
Cuts at: 1563 4140 4513 1563
Size: 2577 373 3063
Fragments arranged by size:
3063 2577 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3117 4076 5460 5728 1632
Size: 1485 959 1384 268 1917
Fragments arranged by size:
1917 1485 1384 959 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5816
AflIII A'CryG_T
Cuts at: 1203 4912 5889 1203
Size: 3709 977 1327
Fragments arranged by size:
3709 1327 977
AgeI A'CCGG_T
Cuts at: 4844 4844
Size: 6013
AhdI GACnn_n'nnGTC
Cuts at: 2974 4742 2974
Size: 1768 4245
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6013
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6013
AlwNI CAG_nnn'CTG
Cuts at: 3046 3756 5328 3046
Size: 710 1572 3731
Fragments arranged by size:
3731 1572 710
ApaI G_GGCC'C
Cuts at: 3071 3071
Size: 6013
ApaLI G'TGCA_C
Cuts at: 4070 5226 4070
Size: 1156 4857
ApoI r'AATT_y
Cuts at: 1493 2420 2708 3240 3322 3548 3700 5809 1493
Size: 927 288 532 82 226 152 2109 1697
Fragments arranged by size:
2109 1697 927 532 288 226 152 82
AseI AT'TA_AT
Cuts at: 160 1032 4567 160
Size: 872 3535 1606
Fragments arranged by size:
3535 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6013
AvaI C'yCGr_G
Cuts at: 1870 1943 2058 3338 1870
Size: 73 115 1280 4545
Fragments arranged by size:
4545 1280 115 73
BamHI G'GATC_C
Cuts at: 2537 3343 3710 2537
Size: 806 367 4840
Fragments arranged by size:
4840 806 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3334 4789 618
Size: 325 217 627 1547 1455 1842
Fragments arranged by size:
1842 1547 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2677 3071 3332 1391
Size: 1286 394 261 4072
Fragments arranged by size:
4072 1286 394 261
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2341 2839 928
Size: 971 442 498 4102
Fragments arranged by size:
4102 971 498 442
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4204 4238 1903
Size: 34 2267 34 3678
Fragments arranged by size:
3678 2267 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3227 3905 5115 3227
Size: 678 1210 4125
Fragments arranged by size:
4125 1210 678
BclI T'GATC_A
Cuts at: 1548 2314 1548
Size: 766 5247
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4624 136
Size: 107 122 71 631 605 2952 1525
Fragments arranged by size:
2952 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2820 6008 2820
Size: 3188 2825
BlpI GC'TnA_GC
Cuts at: 3430 3430
Size: 6013
Bme1580I G_kGCm'C
Cuts at: 1790 3071 4074 5230 1790
Size: 1281 1003 1156 2573
Fragments arranged by size:
2573 1281 1156 1003
BmgBI CAC'GTC
Cuts at: 1318 2937 1318
Size: 1619 4394
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3744 4702 442
Size: 416 53 83 399 24 2327 958 1753
Fragments arranged by size:
2327 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6013
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3004 3004
Size: 6013
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2677 3124 3227 4673 1239
Size: 1438 447 103 1446 2579
Fragments arranged by size:
2579 1446 1438 447 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4138 5003 5301 4138
Size: 865 298 4850
Fragments arranged by size:
4850 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2655 4676 882
Size: 617 1156 2021 2219
Fragments arranged by size:
2219 2021 1156 617
BsaAI yAC'GTr
Cuts at: 493 1204 3310 3804 493
Size: 711 2106 494 2702
Fragments arranged by size:
2702 2106 711 494
BsaBI GATnn'nnATC
Cuts at: 2156 6007 2156
Size: 3851 2162
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3820 4202 4837 275
Size: 53 83 186 936 2287 382 635 1451
Fragments arranged by size:
2287 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3120 4446 4844 5118 5265 5676 3120
Size: 1326 398 274 147 411 3457
Fragments arranged by size:
3457 1326 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5788
Fragments arranged by size:
5788 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3212 3215 1186
Size: 2026 3 3984
Fragments arranged by size:
3984 2026 3
BseYI C'CCAG_C
Cuts at: 5216 5712 5216
Size: 496 5517
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2527 1163
Size: 573 791 4649
Fragments arranged by size:
4649 791 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2503 3463 4224 5252 1540
Size: 275 186 502 960 761 1028 2301
Fragments arranged by size:
2301 1028 960 761 502 275 186
BsiHKAI G_wGCw'C
Cuts at: 4074 4159 5230 4074
Size: 85 1071 4857
Fragments arranged by size:
4857 1071 85
BsmI GAATG_Cn'
Cuts at: 3524 3617 3524
Size: 93 5920
BsmBI CGTCTCn'nnnn_
Cuts at: 2993 2993
Size: 6013
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2677 3071 3332 4074 4159 5230 1391
Size: 399 887 394 261 742 85 1071 2174
Fragments arranged by size:
2174 1071 887 742 399 394 261 85
BspEI T'CCGG_A
Cuts at: 3120 5676 3120
Size: 2556 3457
BspHI T'CATG_A
Cuts at: 3902 5632 3902
Size: 1730 4283
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2290 3368 844
Size: 1446 1078 3489
Fragments arranged by size:
3489 1446 1078
BsrBI CCG'CTC
Cuts at: 1394 1823 2931 3900 1394
Size: 429 1108 969 3507
Fragments arranged by size:
3507 1108 969 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2950 4508 4682 65
Size: 1183 1702 1558 174 1396
Fragments arranged by size:
1702 1558 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4657 4844 1700
Size: 110 2847 187 2869
Fragments arranged by size:
2869 2847 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6013
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6013
BssSI C'ACGA_G
Cuts at: 4073 5085 4073
Size: 1012 5001
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2940 794
Size: 2146 3867
BstBI TT'CG_AA
Cuts at: 2007 4817 2007
Size: 2810 3203
BstEII G'GTnAC_C
Cuts at: 1184 2871 1184
Size: 1687 4326
BstYI r'GATC_y
Cuts at: 1972 2537 2820 3343 3710 4095 4112 5564 5650
Size: 565 283 523 367 385 17 1452 86
Cuts at: 5650 5662 6008 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 565 523 385 367 346 283
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2094 2094
Size: 6013
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4504
Fragments arranged by size:
4504 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5274
Fragments arranged by size:
5274 683 56
BtsI GCAGTG_nn'
Cuts at: 2881 3529 4323 4343 2881
Size: 648 794 20 4551
Fragments arranged by size:
4551 794 648 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6013
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5978
DraI TTT'AAA
Cuts at: 3317 3317
Size: 6013
DraIII CAC_nnn'GTG
Cuts at: 2764 2764
Size: 6013
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5020 809
Size: 507 3704 1802
Fragments arranged by size:
3704 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3460 4349 5978
Size: 54 1425 50 275 18 1630 889 1629
Cuts at: 5978 8
Size: 43
Fragments arranged by size:
1630 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3460 1537
Size: 275 1648 4090
Fragments arranged by size:
4090 1648 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3943 1353
Size: 2590 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3118 4600 4974 5120 387
Size: 1064 138 1529 1482 374 146 1280
Fragments arranged by size:
1529 1482 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2677 3117 3124 3227 4076 4673 5460
Size: 393 1045 440 7 103 849 597 787
Cuts at: 5460 5728 1239
Size: 268 1524
Fragments arranged by size:
1524 1045 849 787 597 440 393 268
103 7
EcoO109I rG'GnC_Cy
Cuts at: 3067 3068 3067
Size: 1 6012
EcoRI G'AATT_C
Cuts at: 2420 3322 3700 2420
Size: 902 378 4733
Fragments arranged by size:
4733 902 378
FspI TGC'GCA
Cuts at: 4519 4519
Size: 6013
HaeII r_GCGC'y
Cuts at: 1678 5160 1678
Size: 3482 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2688 2720 2726 2758 2960
Size: 32 135 32 1116 32 6 32 202
Cuts at: 2960 2992 3253 3285 4654 4686 4728 4760 1373
Size: 32 261 32 1369 32 42 32 2626
Fragments arranged by size:
2626 1369 1116 261 202 135 42 32
32 32 32 32 32 32 32 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3357 3603 669
Size: 988 281 1419 246 3079
Fragments arranged by size:
3079 1419 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6013
HpaI GTT'AAC
Cuts at: 3603 3603
Size: 6013
KpnI G_GTAC'C
Cuts at: 3338 3338
Size: 6013
MfeI C'AATT_G
Cuts at: 3192 3612 3192
Size: 420 5593
MluI A'CGCG_T
Cuts at: 5889 5889
Size: 6013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3109 5127 5311 1305
Size: 1804 2018 184 2007
Fragments arranged by size:
2018 2007 1804 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4191
Fragments arranged by size:
4191 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2522 3971 4330 4489 518
Size: 648 1356 1449 359 159 2042
Fragments arranged by size:
2042 1449 1356 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3079 3429 4106 5254 5499 1606
Size: 1473 350 677 1148 245 2120
Fragments arranged by size:
2120 1473 1148 677 350 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4504
Fragments arranged by size:
4504 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6013
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6013
NotI GC'GGCC_GC
Cuts at: 3460 3460
Size: 6013
NspI r_CATG'y
Cuts at: 1132 1229 3371 4916 1132
Size: 97 2142 1545 2229
Fragments arranged by size:
2229 2142 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6013
PciI A'CATG_T
Cuts at: 4912 4912
Size: 6013
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2764 3046 1108
Size: 1656 282 4075
Fragments arranged by size:
4075 1656 282
PfoI T'CCnGG_A
Cuts at: 3219 3219
Size: 6013
PmeI GTTT'AAAC
Cuts at: 3317 3317
Size: 6013
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2011 2043 4082 4114 5595 5627 2011
Size: 32 2039 32 1481 32 2397
Fragments arranged by size:
2397 2039 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6013
PsiI TTA'TAA
Cuts at: 3583 3583
Size: 6013
PspOMI G'GGCC_C
Cuts at: 3067 3067
Size: 6013
PstI C_TGCA'G
Cuts at: 830 3365 830
Size: 2535 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6013
PvuII CAG'CTG
Cuts at: 3079 3079
Size: 6013
SalI G'TCGA_C
Cuts at: 1936 3355 1936
Size: 1419 4594
SbfI CC_TGCA'GG
Cuts at: 3365 3365
Size: 6013
ScaI AGT'ACT
Cuts at: 4261 5973 4261
Size: 1712 4301
SfcI C'TryA_G
Cuts at: 826 1045 2041 3361 4496 5177 5368 826
Size: 219 996 1320 1135 681 191 1471
Fragments arranged by size:
1471 1320 1135 996 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6013
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6013
SmaI CCC'GGG
Cuts at: 1872 3340 1872
Size: 1468 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2058 4117 5018 5280 820
Size: 197 926 115 2059 901 262 1553
Fragments arranged by size:
2059 1553 926 901 262 197 115
SnaBI TAC'GTA
Cuts at: 493 3310 3804 493
Size: 2817 494 2702
Fragments arranged by size:
2817 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6013
SphI G_CATG'C
Cuts at: 1132 3371 1132
Size: 2239 3774
SspI AAT'ATT
Cuts at: 5 52 3937 5
Size: 47 3885 2081
Fragments arranged by size:
3885 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6013
StyI C'CwwG_G
Cuts at: 513 1065 2022 2220 2229 2452 2547 2619 3272
Size: 552 957 198 9 223 95 72 653
Cuts at: 3272 3452 513
Size: 180 3074
Fragments arranged by size:
3074 957 653 552 223 198 180 95
72 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4040 4057 4210 4395 4840 325
Size: 1255 2460 17 153 185 445 1498
Fragments arranged by size:
2460 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4259 5971 96
Size: 274 80 33 51 1443 2282 1712 138
Fragments arranged by size:
2282 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5973 6005 5973
Size: 32 5981
Tth111I GACn'n_nGTC
Cuts at: 1316 2375 1316
Size: 1059 4954
XbaI T'CTAG_A
Cuts at: 3349 3349
Size: 6013
XhoI C'TCGA_G
Cuts at: 1943 2058 1943
Size: 115 5898
XmaI C'CCGG_G
Cuts at: 1870 3338 1870
Size: 1468 4545
XmnI GAAnn'nnTTC
Cuts at: 2614 4142 2614
Size: 1528 4485
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3821 276
Size: 53 83 186 936 2287 2468
Fragments arranged by size:
2468 2287 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstXI BstZ17I EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI