Restriction map for
pFN21ASDA1279
(Circular) (Six-base) MAPSORT of: pFN21ASDA1279.seq Check: 7530 from: 1 to: 6592
pFN21ASDA1279 6592 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4402 278
Size: 53 83 186 936 2866 2468
Fragments arranged by size:
2866 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2673 3399 3935 1937
Size: 736 726 536 4594
Fragments arranged by size:
4594 736 726 536
Acc65I G'GTAC_C
Cuts at: 2403 3913 2403
Size: 1510 5082
AclI AA'CG_TT
Cuts at: 1563 2031 4719 5092 1563
Size: 468 2688 373 3063
Fragments arranged by size:
3063 2688 468 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2610 2922 2928 3584 4655 6039 6307 1632
Size: 978 312 6 656 1071 1384 268 1917
Fragments arranged by size:
1917 1384 1071 978 656 312 268 6
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6395
AflIII A'CryG_T
Cuts at: 1203 5491 6468 1203
Size: 4288 977 1327
Fragments arranged by size:
4288 1327 977
AgeI A'CCGG_T
Cuts at: 3122 5423 3122
Size: 2301 4291
AhdI GACnn_n'nnGTC
Cuts at: 5321 5321
Size: 6592
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6592
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2065 1184
Size: 881 5711
AlwNI CAG_nnn'CTG
Cuts at: 2683 2835 4335 5907 2683
Size: 152 1500 1572 3368
Fragments arranged by size:
3368 1572 1500 152
ApaI G_GGCC'C
Cuts at: 2280 2280
Size: 6592
ApaLI G'TGCA_C
Cuts at: 4649 5805 4649
Size: 1156 5436
ApoI r'AATT_y
Cuts at: 1493 2057 3724 3752 3901 4127 4279 6388 1493
Size: 564 1667 28 149 226 152 2109 1697
Fragments arranged by size:
2109 1697 1667 564 226 152 149 28
AseI AT'TA_AT
Cuts at: 160 1032 5146 160
Size: 872 4114 1606
Fragments arranged by size:
4114 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6592
AvaI C'yCGr_G
Cuts at: 1870 1943 2139 2221 2270 2324 3917 1870
Size: 73 196 82 49 54 1593 4545
Fragments arranged by size:
4545 1593 196 82 73 54 49
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2443 2476 2443
Size: 33 6559
BamHI G'GATC_C
Cuts at: 3511 3922 4289 3511
Size: 411 367 5814
Fragments arranged by size:
5814 411 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2225 2403 3913 5368 618
Size: 325 217 627 438 178 1510 1455 1842
Fragments arranged by size:
1842 1510 1455 627 438 325 217 178
BanII G_rGCy'C
Cuts at: 1391 2271 2280 3390 3911 1391
Size: 880 9 1110 521 4072
Fragments arranged by size:
4072 1110 880 521 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2898 3830 928
Size: 971 999 932 3690
Fragments arranged by size:
3690 999 971 932
BbvCI CC'TCA_GC
Cuts at: 2256 2256
Size: 6592
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4783 4817 1903
Size: 34 2846 34 3678
Fragments arranged by size:
3678 2846 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4484 5694 4484
Size: 1210 5382
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6592
BfrBI ATG'CAT
Cuts at: 3361 3361
Size: 6592
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2275 5203 136
Size: 107 122 71 631 605 603 2928 1525
Fragments arranged by size:
2928 1525 631 605 603 122 107 71
BglII A'GATC_T
Cuts at: 2877 6587 2877
Size: 3710 2882
BlpI GC'TnA_GC
Cuts at: 4009 4009
Size: 6592
Bme1580I G_kGCm'C
Cuts at: 1790 2280 4653 5809 1790
Size: 490 2373 1156 2573
Fragments arranged by size:
2573 2373 1156 490
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6592
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4323 5281 442
Size: 416 53 83 399 24 2906 958 1753
Fragments arranged by size:
2906 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6592
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2577 2577
Size: 6592
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5252 1239
Size: 4013 2579
Bpu10I CC'TnA_GC
Cuts at: 2256 3506 3618 2256
Size: 1250 112 5230
Fragments arranged by size:
5230 1250 112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2652 2759 3193 4717 5582 5880 2652
Size: 107 434 1524 865 298 3364
Fragments arranged by size:
3364 1524 865 434 298 107
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3169 3591 5255 882
Size: 617 1670 422 1664 2219
Fragments arranged by size:
2219 1670 1664 617 422
BsaAI yAC'GTr
Cuts at: 493 1204 3889 4383 493
Size: 711 2685 494 2702
Fragments arranged by size:
2702 2685 711 494
BsaBI GATnn'nnATC
Cuts at: 6586 6586
Size: 6592
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4399 4781 5416 275
Size: 53 83 186 936 2866 382 635 1451
Fragments arranged by size:
2866 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2104 3122 5025 5423 5697 5844 6255 2104
Size: 1018 1903 398 274 147 411 2441
Fragments arranged by size:
2441 1903 1018 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2339 2369 3335 3365 1178
Size: 30 165 30 936 30 966 30 4405
Fragments arranged by size:
4405 966 936 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2363 2381 2550 3098 1186
Size: 1177 18 169 548 4680
Fragments arranged by size:
4680 1177 548 169 18
BseYI C'CCAG_C
Cuts at: 2286 5795 6291 2286
Size: 3509 496 2587
Fragments arranged by size:
3509 2587 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2478 3131 1163
Size: 573 742 653 4624
Fragments arranged by size:
4624 742 653 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4042 4803 5831 1540
Size: 275 186 2041 761 1028 2301
Fragments arranged by size:
2301 2041 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2373 2745 3307 4653 4738 5809 2373
Size: 372 562 1346 85 1071 3156
Fragments arranged by size:
3156 1346 1071 562 372 85
BsmI GAATG_Cn'
Cuts at: 2998 3093 3323 4103 4196 2998
Size: 95 230 780 93 5394
Fragments arranged by size:
5394 780 230 95 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3340 3683 3340
Size: 343 6249
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2271 2280 2373 2745 3307 3390 3911
Size: 399 481 9 93 372 562 83 521
Cuts at: 3911 4653 4738 5809 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 562 521 481 399 372
93 85 83 9
BspEI T'CCGG_A
Cuts at: 2104 6255 2104
Size: 4151 2441
BspHI T'CATG_A
Cuts at: 4481 6211 4481
Size: 1730 4862
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2391 3947 844
Size: 1547 1556 3489
Fragments arranged by size:
3489 1556 1547
BsrBI CCG'CTC
Cuts at: 1394 1823 2200 2294 4479 1394
Size: 429 377 94 2185 3507
Fragments arranged by size:
3507 2185 429 377 94
BsrDI GCAATG_nn'
Cuts at: 65 1248 3492 5087 5261 65
Size: 1183 2244 1595 174 1396
Fragments arranged by size:
2244 1595 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2228 2251 2406 3122 5236 5423 1700
Size: 110 418 23 155 716 2114 187 2869
Fragments arranged by size:
2869 2114 716 418 187 155 110 23
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6592
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6592
BssSI C'ACGA_G
Cuts at: 4652 5664 4652
Size: 1012 5580
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6592
BstBI TT'CG_AA
Cuts at: 2007 5396 2007
Size: 3389 3203
BstEII G'GTnAC_C
Cuts at: 1184 2231 1184
Size: 1047 5545
BstYI r'GATC_y
Cuts at: 1972 2877 3438 3511 3703 3922 4289 4674 4691
Size: 905 561 73 192 219 367 385 17
Cuts at: 4691 6143 6229 6241 6587 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 905 561 385 367 346 219
192 86 73 17 12
Bsu36I CC'TnA_GG
Cuts at: 2184 2205 3783 2184
Size: 21 1578 4993
Fragments arranged by size:
4993 1578 21
BtgI C'CryG_G
Cuts at: 513 1065 2022 2036 513
Size: 552 957 14 5069
Fragments arranged by size:
5069 957 552 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5853
Fragments arranged by size:
5853 683 56
BtsI GCAGTG_nn'
Cuts at: 4108 4902 4922 4108
Size: 794 20 5778
Fragments arranged by size:
5778 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6592
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6557
DraI TTT'AAA
Cuts at: 3896 3896
Size: 6592
DraIII CAC_nnn'GTG
Cuts at: 2377 2377
Size: 6592
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5599 809
Size: 507 4283 1802
Fragments arranged by size:
4283 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4039 4928 6557
Size: 54 1425 50 275 18 2209 889 1629
Cuts at: 6557 8
Size: 43
Fragments arranged by size:
2209 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4039 1537
Size: 275 2227 4090
Fragments arranged by size:
4090 2227 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2148 2589 3150 3584 4522 1353
Size: 795 441 561 434 938 3423
Fragments arranged by size:
3423 938 795 561 441 434
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5179 5553 5699 387
Size: 1064 138 3590 374 146 1280
Fragments arranged by size:
3590 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2610 2922 2928 3584 4655 5252 6039
Size: 393 978 312 6 656 1071 597 787
Cuts at: 6039 6307 1239
Size: 268 1524
Fragments arranged by size:
1524 1071 978 787 656 597 393 312
268 6
EcoO109I rG'GnC_Cy
Cuts at: 2276 2276
Size: 6592
EcoRI G'AATT_C
Cuts at: 3901 4279 3901
Size: 378 6214
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2618 2833 3656 2618
Size: 215 823 5554
Fragments arranged by size:
5554 823 215
FspI TGC'GCA
Cuts at: 2399 5098 2399
Size: 2699 3893
HaeII r_GCGC'y
Cuts at: 1678 2072 2139 2147 2168 5739 1678
Size: 394 67 8 21 3571 2531
Fragments arranged by size:
3571 2531 394 67 21 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2339 2371 2411 2443 3029
Size: 32 135 32 767 32 40 32 586
Cuts at: 3029 3061 3128 3160 3820 3852 5233 5265 5307
Size: 32 67 32 660 32 1381 32 42
Cuts at: 5307 5339 1373
Size: 32 2626
Fragments arranged by size:
2626 1381 767 660 586 135 67 42
40 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3430 3936 4182 669
Size: 988 281 1492 506 246 3079
Fragments arranged by size:
3079 1492 988 506 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6592
HpaI GTT'AAC
Cuts at: 4182 4182
Size: 6592
KpnI G_GTAC'C
Cuts at: 2407 3917 2407
Size: 1510 5082
MfeI C'AATT_G
Cuts at: 4191 4191
Size: 6592
MluI A'CGCG_T
Cuts at: 6468 6468
Size: 6592
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5706 5890 1305
Size: 4401 184 2007
Fragments arranged by size:
4401 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4770
Fragments arranged by size:
4770 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4550 4909 5068 518
Size: 648 3384 359 159 2042
Fragments arranged by size:
3384 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2846 4008 4685 5833 6078 1606
Size: 1240 1162 677 1148 245 2120
Fragments arranged by size:
2120 1240 1162 1148 677 245
NaeI GCC'GGC
Cuts at: 2253 2253
Size: 6592
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5083
Fragments arranged by size:
5083 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6592
NgoMIV G'CCGG_C
Cuts at: 2251 2251
Size: 6592
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6592
NotI GC'GGCC_GC
Cuts at: 4039 4039
Size: 6592
NsiI A_TGCA'T
Cuts at: 3363 3363
Size: 6592
NspI r_CATG'y
Cuts at: 1132 1229 3461 3950 5495 1132
Size: 97 2232 489 1545 2229
Fragments arranged by size:
2232 2229 1545 489 97
PasI CC'CwG_GG
Cuts at: 1348 2242 1348
Size: 894 5698
PciI A'CATG_T
Cuts at: 5491 5491
Size: 6592
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2242 2683 1108
Size: 1134 441 5017
Fragments arranged by size:
5017 1134 441
PmeI GTTT'AAAC
Cuts at: 3896 3896
Size: 6592
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4661 4693 6174 6206 4661
Size: 32 1481 32 5047
Fragments arranged by size:
5047 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6592
PsiI TTA'TAA
Cuts at: 4162 4162
Size: 6592
PspOMI G'GGCC_C
Cuts at: 2276 2276
Size: 6592
PstI C_TGCA'G
Cuts at: 830 3151 3703 3944 830
Size: 2321 552 241 3478
Fragments arranged by size:
3478 2321 552 241
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6592
PvuII CAG'CTG
Cuts at: 2846 2846
Size: 6592
SalI G'TCGA_C
Cuts at: 1936 3934 1936
Size: 1998 4594
SapI GCTCTTCn'nnn_
Cuts at: 3584 3584
Size: 6592
SbfI CC_TGCA'GG
Cuts at: 3944 3944
Size: 6592
ScaI AGT'ACT
Cuts at: 3569 4840 6552 3569
Size: 1271 1712 3609
Fragments arranged by size:
3609 1712 1271
SexAI A'CCwGG_T
Cuts at: 2373 2373
Size: 6592
SfcI C'TryA_G
Cuts at: 826 1045 2760 3147 3396 3699 3940 5075 5756
Size: 219 1715 387 249 303 241 1135 681
Cuts at: 5756 5947 826
Size: 191 1471
Fragments arranged by size:
1715 1471 1135 681 387 303 249 241
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6592
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6592
SmaI CCC'GGG
Cuts at: 1872 2141 2223 3919 1872
Size: 269 82 1696 4545
Fragments arranged by size:
4545 1696 269 82
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2667 2738 3172 4696 5597 5859
Size: 197 926 724 71 434 1524 901 262
Cuts at: 5859 820
Size: 1553
Fragments arranged by size:
1553 1524 926 901 724 434 262 197
71
SnaBI TAC'GTA
Cuts at: 493 3889 4383 493
Size: 3396 494 2702
Fragments arranged by size:
3396 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6592
SphI G_CATG'C
Cuts at: 1132 3950 1132
Size: 2818 3774
SrfI GCCC'GGGC
Cuts at: 2141 2141
Size: 6592
SspI AAT'ATT
Cuts at: 5 52 4516 5
Size: 47 4464 2081
Fragments arranged by size:
4464 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6592
StyI C'CwwG_G
Cuts at: 513 1065 2022 4031 513
Size: 552 957 2009 3074
Fragments arranged by size:
3074 2009 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4619 4636 4789 4974 5419 325
Size: 1255 3039 17 153 185 445 1498
Fragments arranged by size:
3039 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2720 3567 4838
Size: 274 80 33 51 1443 743 847 1271
Cuts at: 4838 6550 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1271 847 743 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3335 3367 6552 6584 3335
Size: 32 3185 32 3343
Fragments arranged by size:
3343 3185 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6592
XbaI T'CTAG_A
Cuts at: 3928 3928
Size: 6592
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3715 3715
Size: 6592
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6592
XmaI C'CCGG_G
Cuts at: 1870 2139 2221 3917 1870
Size: 269 82 1696 4545
Fragments arranged by size:
4545 1696 269 82
XmnI GAAnn'nnTTC
Cuts at: 3093 3636 4721 3093
Size: 543 1085 4964
Fragments arranged by size:
4964 1085 543
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4400 276
Size: 53 83 186 936 2866 2468
Fragments arranged by size:
2866 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BsiWI BstXI
BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI NarI
NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SfiI SfoI SwaI