Restriction map for
pFN21ASDA1276
(Circular) (Six-base) MAPSORT of: pFN21ASDA1276.seq Check: 955 from: 1 to: 7909
pFN21ASDA1276 7909 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5719 278
Size: 53 83 186 936 4183 2468
Fragments arranged by size:
4183 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5087 5252 1937
Size: 3150 165 4594
Fragments arranged by size:
4594 3150 165
Acc65I G'GTAC_C
Cuts at: 5230 5230
Size: 7909
AclI AA'CG_TT
Cuts at: 1563 6036 6409 1563
Size: 4473 373 3063
Fragments arranged by size:
4473 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2900 2921 3705 4727 4793 5972 7356 7624
Size: 1268 21 784 1022 66 1179 1384 268
Cuts at: 7624 1632
Size: 1917
Fragments arranged by size:
1917 1384 1268 1179 1022 784 268 66
21
AflII C'TTAA_G
Cuts at: 820 1017 2933 820
Size: 197 1916 5796
Fragments arranged by size:
5796 1916 197
AflIII A'CryG_T
Cuts at: 1203 6808 7785 1203
Size: 5605 977 1327
Fragments arranged by size:
5605 1327 977
AgeI A'CCGG_T
Cuts at: 6740 6740
Size: 7909
AhdI GACnn_n'nnGTC
Cuts at: 6638 6638
Size: 7909
AleI CACnn'nnGTG
Cuts at: 1166 2162 1166
Size: 996 6913
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3069 3203 4263 1184
Size: 1885 134 1060 4830
Fragments arranged by size:
4830 1885 1060 134
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4949 4981 4949
Size: 32 7877
AlwNI CAG_nnn'CTG
Cuts at: 2332 2515 2629 2650 2752 2899 2920 3563 3868
Size: 183 114 21 102 147 21 643 305
Cuts at: 3868 4204 4231 5652 7224 2332
Size: 336 27 1421 1572 3017
Fragments arranged by size:
3017 1572 1421 643 336 305 183 147
114 102 27 21 21
ApaI G_GGCC'C
Cuts at: 2594 2594
Size: 7909
ApaLI G'TGCA_C
Cuts at: 3877 5966 7122 3877
Size: 2089 1156 4664
Fragments arranged by size:
4664 2089 1156
ApoI r'AATT_y
Cuts at: 1493 2953 3496 3527 5218 5444 5596 7705 1493
Size: 1460 543 31 1691 226 152 2109 1697
Fragments arranged by size:
2109 1697 1691 1460 543 226 152 31
AseI AT'TA_AT
Cuts at: 160 1032 5010 6463 160
Size: 872 3978 1453 1606
Fragments arranged by size:
3978 1606 1453 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7909
AvaI C'yCGr_G
Cuts at: 1870 1943 3998 4381 4733 4885 5234 1870
Size: 73 2055 383 352 152 349 4545
Fragments arranged by size:
4545 2055 383 352 349 152 73
BamHI G'GATC_C
Cuts at: 3619 5239 5606 3619
Size: 1620 367 5922
Fragments arranged by size:
5922 1620 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2090 4260 5230 6685 618
Size: 325 217 627 303 2170 970 1455 1842
Fragments arranged by size:
2170 1842 1455 970 627 325 303 217
BanII G_rGCy'C
Cuts at: 1391 2075 2244 2484 2564 2594 3767 3957 4021
Size: 684 169 240 80 30 1173 190 64
Cuts at: 4021 4134 4303 4443 5165 5228 1391
Size: 113 169 140 722 63 4072
Fragments arranged by size:
4072 1173 722 684 240 190 169 169
140 113 80 64 63 30
BbeI G_GCGC'C
Cuts at: 2094 2094
Size: 7909
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3068 3252 3327 928
Size: 971 1169 184 75 5510
Fragments arranged by size:
5510 1169 971 184 75
BbvCI CC'TCA_GC
Cuts at: 2199 2423 3132 3564 4295 2199
Size: 224 709 432 731 5813
Fragments arranged by size:
5813 731 709 432 224
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2128 2162 3014 3048 6100 6134 1903
Size: 34 191 34 852 34 3052 34 3678
Fragments arranged by size:
3678 3052 852 191 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5801 7011 5801
Size: 1210 6699
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7909
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2672 2819 4392
Size: 107 122 71 631 605 1000 147 1573
Cuts at: 4392 4571 4628 4670 6520 136
Size: 179 57 42 1850 1525
Fragments arranged by size:
1850 1573 1525 1000 631 605 179 147
122 107 71 57 42
BglII A'GATC_T
Cuts at: 7904 7904
Size: 7909
BlpI GC'TnA_GC
Cuts at: 2651 5326 2651
Size: 2675 5234
Bme1580I G_kGCm'C
Cuts at: 1790 2594 3164 3881 4263 4648 4935 5970 7126
Size: 804 570 717 382 385 287 1035 1156
Cuts at: 7126 1790
Size: 2573
Fragments arranged by size:
2573 1156 1035 804 717 570 385 382
287
BmgBI CAC'GTC
Cuts at: 1318 4930 1318
Size: 3612 4297
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3770 4833 5640
Size: 416 53 83 399 24 2353 1063 807
Cuts at: 5640 6598 442
Size: 958 1753
Fragments arranged by size:
2353 1753 1063 958 807 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7909
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 7909
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2119 2228 2354 2366 2423 2449 2492 2498
Size: 880 109 126 12 57 26 43 6
Cuts at: 2498 2870 3476 3629 3890 3941 3950 4052 4085
Size: 372 606 153 261 51 9 102 33
Cuts at: 4085 4118 4148 4448 4568 4643 6569 1239
Size: 33 30 300 120 75 1926 2579
Fragments arranged by size:
2579 1926 880 606 372 300 261 153
126 120 109 102 75 57 51 43
33 33 30 26 12 9 6
Bpu10I CC'TnA_GC
Cuts at: 2199 2423 2823 3132 3564 4295 2199
Size: 224 400 309 432 731 5813
Fragments arranged by size:
5813 731 432 400 309 224
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2468 3137 3527 3716 3896 4040 6034 6899 7197
Size: 669 390 189 180 144 1994 865 298
Cuts at: 7197 2468
Size: 3180
Fragments arranged by size:
3180 1994 865 669 390 298 189 180
144
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2255 2838 3605 3853 3956 4524 6572
Size: 617 756 583 767 248 103 568 2048
Cuts at: 6572 882
Size: 2219
Fragments arranged by size:
2219 2048 767 756 617 583 568 248
103
BsaAI yAC'GTr
Cuts at: 493 1204 2128 5206 5700 493
Size: 711 924 3078 494 2702
Fragments arranged by size:
3078 2702 924 711 494
BsaBI GATnn'nnATC
Cuts at: 7903 7903
Size: 7909
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2091 5716 6098 6733
Size: 53 83 186 936 558 3625 382 635
Cuts at: 6733 275
Size: 1451
Fragments arranged by size:
3625 1451 936 635 558 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2829 4605 4700 4854 6342 6740 7014 7161 7572
Size: 1776 95 154 1488 398 274 147 411
Cuts at: 7572 2829
Size: 3166
Fragments arranged by size:
3166 1776 1488 411 398 274 154 147
95
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2759 2789 4116 4146 4949
Size: 30 165 30 1356 30 1327 30 803
Cuts at: 4949 4979 1178
Size: 30 4108
Fragments arranged by size:
4108 1356 1327 803 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2252 2315 2384 2515 2693 3293 3669 3674
Size: 1066 63 69 131 178 600 376 5
Cuts at: 3674 3821 4187 4382 4463 4565 1186
Size: 147 366 195 81 102 4530
Fragments arranged by size:
4530 1066 600 376 366 195 178 147
131 102 81 69 63 5
BseYI C'CCAG_C
Cuts at: 2151 3993 4412 4566 4653 4934 5036 5054 5148
Size: 1842 419 154 87 281 102 18 94
Cuts at: 5148 7112 7608 2151
Size: 1964 496 2452
Fragments arranged by size:
2452 1964 1842 496 419 281 154 102
94 87 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2416 2642 2948 1163
Size: 573 680 226 306 6124
Fragments arranged by size:
6124 680 573 306 226
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5359 6120 7148 1540
Size: 275 186 3358 761 1028 2301
Fragments arranged by size:
3358 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2244 2442 2484 2856 3881 3957 4134 5970 6055
Size: 198 42 372 1025 76 177 1836 85
Cuts at: 6055 7126 2244
Size: 1071 3027
Fragments arranged by size:
3027 1836 1071 1025 372 198 177 85
76 42
BsmI GAATG_Cn'
Cuts at: 2254 3603 4832 5420 5513 2254
Size: 1349 1229 588 93 4650
Fragments arranged by size:
4650 1349 1229 588 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2399 2399
Size: 7909
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2075 2244 2442 2484 2564 2594 2856
Size: 399 285 169 198 42 80 30 262
Cuts at: 2856 3164 3767 3881 3957 4021 4134 4263 4303
Size: 308 603 114 76 64 113 129 40
Cuts at: 4303 4443 4648 4935 5165 5228 5970 6055 7126
Size: 140 205 287 230 63 742 85 1071
Cuts at: 7126 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 603 399 308 287 285
262 230 205 198 169 140 129 114
113 85 80 76 64 63 42 40
30
BspEI T'CCGG_A
Cuts at: 2829 4700 7572 2829
Size: 1871 2872 3166
Fragments arranged by size:
3166 2872 1871
BspHI T'CATG_A
Cuts at: 5798 7528 5798
Size: 1730 6179
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3909 4530 5264 844
Size: 3065 621 734 3489
Fragments arranged by size:
3489 3065 734 621
BsrBI CCG'CTC
Cuts at: 1394 1823 5796 1394
Size: 429 3973 3507
Fragments arranged by size:
3973 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6404 6578 65
Size: 1183 5156 174 1396
Fragments arranged by size:
5156 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2093 2544 3116 4391 6553 6740 1700
Size: 110 283 451 572 1275 2162 187 2869
Fragments arranged by size:
2869 2162 1275 572 451 283 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7909
BssHII G'CGCG_C
Cuts at: 1925 4486 1925
Size: 2561 5348
BssSI C'ACGA_G
Cuts at: 2392 2525 5969 6981 2392
Size: 133 3444 1012 3320
Fragments arranged by size:
3444 3320 1012 133
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2629 2728 4165 4204 5102 794
Size: 1835 99 1437 39 898 3601
Fragments arranged by size:
3601 1835 1437 898 99 39
BstBI TT'CG_AA
Cuts at: 2007 6713 2007
Size: 4706 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7909
BstXI CCAn_nnnn'nTGG
Cuts at: 4582 4942 4582
Size: 360 7549
BstYI r'GATC_y
Cuts at: 1972 3619 4104 5239 5606 5991 6008 7460 7546
Size: 1647 485 1135 367 385 17 1452 86
Cuts at: 7546 7558 7904 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1647 1452 1135 485 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 3342 3369 4623 513
Size: 552 957 1320 27 1254 3799
Fragments arranged by size:
3799 1320 1254 957 552 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7170
Fragments arranged by size:
7170 683 56
BtsI GCAGTG_nn'
Cuts at: 2765 2970 3595 3706 3881 4280 4589 5425 6219
Size: 205 625 111 175 399 309 836 794
Cuts at: 6219 6239 2765
Size: 20 4435
Fragments arranged by size:
4435 836 794 625 399 309 205 175
111 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7909
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7874
DraI TTT'AAA
Cuts at: 5213 5213
Size: 7909
DraIII CAC_nnn'GTG
Cuts at: 3343 3343
Size: 7909
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6916 809
Size: 507 5600 1802
Fragments arranged by size:
5600 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4753 5062 5356
Size: 54 1425 50 275 18 2923 309 294
Cuts at: 5356 6245 7874 8
Size: 889 1629 43
Fragments arranged by size:
2923 1629 1425 889 309 294 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5356 1537
Size: 275 3544 4090
Fragments arranged by size:
4090 3544 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2351 3093 3270 3459 4803 5839 1353
Size: 998 742 177 189 1344 1036 3423
Fragments arranged by size:
3423 1344 1036 998 742 189 177
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6496 6870 7016 387
Size: 1064 138 4907 374 146 1280
Fragments arranged by size:
4907 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2242 2482 3955 4132 2242
Size: 240 1473 177 6019
Fragments arranged by size:
6019 1473 240 177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2119 2228 2354 2366 2423 2449 2492
Size: 393 487 109 126 12 57 26 43
Cuts at: 2492 2498 2870 2900 2921 3476 3629 3705 3890
Size: 6 372 30 21 555 153 76 185
Cuts at: 3890 3941 3950 4052 4085 4118 4148 4448 4568
Size: 51 9 102 33 33 30 300 120
Cuts at: 4568 4643 4727 4793 5972 6569 7356 7624 1239
Size: 75 84 66 1179 597 787 268 1524
Fragments arranged by size:
1524 1179 787 597 555 487 393 372
300 268 185 153 126 120 109 102
84 76 75 66 57 51 43 33
33 30 30 26 21 12 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2395 2615 2698 2395
Size: 220 83 7606
Fragments arranged by size:
7606 220 83
EcoO109I rG'GnC_Cy
Cuts at: 2329 2590 2608 2749 3898 4201 4738 5112 2329
Size: 261 18 141 1149 303 537 374 5126
Fragments arranged by size:
5126 1149 537 374 303 261 141 18
EcoRI G'AATT_C
Cuts at: 2953 3496 5218 5596 2953
Size: 543 1722 378 5266
Fragments arranged by size:
5266 1722 543 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3266 3266
Size: 7909
FspI TGC'GCA
Cuts at: 2296 4025 6415 2296
Size: 1729 2390 3790
Fragments arranged by size:
3790 2390 1729
HaeII r_GCGC'y
Cuts at: 1678 2094 2219 2343 2378 4410 7056 1678
Size: 416 125 124 35 2032 2646 2531
Fragments arranged by size:
2646 2531 2032 416 125 124 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2759 2791 3604 3636 3770
Size: 32 135 32 1187 32 813 32 134
Cuts at: 3770 3802 4116 4148 4425 4457 4773 4805 4978
Size: 32 314 32 277 32 316 32 173
Cuts at: 4978 5010 6550 6582 6624 6656 1373
Size: 32 1540 32 42 32 2626
Fragments arranged by size:
2626 1540 1187 813 316 314 277 173
135 134 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5253 5499 669
Size: 988 281 3315 246 3079
Fragments arranged by size:
3315 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7909
HpaI GTT'AAC
Cuts at: 5499 5499
Size: 7909
KasI G'GCGC_C
Cuts at: 2090 2090
Size: 7909
KpnI G_GTAC'C
Cuts at: 5234 5234
Size: 7909
MfeI C'AATT_G
Cuts at: 5508 5508
Size: 7909
MluI A'CGCG_T
Cuts at: 7785 7785
Size: 7909
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2556 2757 3396 3559 4132 4215 7023 7207
Size: 1251 201 639 163 573 83 2808 184
Cuts at: 7207 1305
Size: 2007
Fragments arranged by size:
2808 2007 1251 639 573 201 184 163
83
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6087
Fragments arranged by size:
6087 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2162 2922 3185 5867 6226 6385 518
Size: 648 996 760 263 2682 359 159 2042
Fragments arranged by size:
2682 2042 996 760 648 359 263 159
MspA1I CmG'CkG
Cuts at: 1606 2626 2650 3457 3683 4570 5058 5325 6002
Size: 1020 24 807 226 887 488 267 677
Cuts at: 6002 7150 7395 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1020 887 807 677 488 267
245 226 24
NaeI GCC'GGC
Cuts at: 2095 2546 3118 4393 2095
Size: 451 572 1275 5611
Fragments arranged by size:
5611 1275 572 451
NarI GG'CG_CC
Cuts at: 2091 2091
Size: 7909
NcoI C'CATG_G
Cuts at: 513 1065 2022 4623 513
Size: 552 957 2601 3799
Fragments arranged by size:
3799 2601 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7909
NgoMIV G'CCGG_C
Cuts at: 2093 2544 3116 4391 2093
Size: 451 572 1275 5611
Fragments arranged by size:
5611 1275 572 451
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7909
NotI GC'GGCC_GC
Cuts at: 5356 5356
Size: 7909
NspI r_CATG'y
Cuts at: 1132 1229 4030 5267 6812 1132
Size: 97 2801 1237 1545 2229
Fragments arranged by size:
2801 2229 1545 1237 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7909
PciI A'CATG_T
Cuts at: 6808 6808
Size: 7909
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2332 4141 1108
Size: 1224 1809 4876
Fragments arranged by size:
4876 1809 1224
PfoI T'CCnGG_A
Cuts at: 2802 3957 4333 4464 4545 5003 2802
Size: 1155 376 131 81 458 5708
Fragments arranged by size:
5708 1155 458 376 131 81
PmeI GTTT'AAAC
Cuts at: 5213 5213
Size: 7909
PmlI CAC'GTG
Cuts at: 2128 2128
Size: 7909
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4949 4981 5978 6010 7491 7523 4949
Size: 32 997 32 1481 32 5335
Fragments arranged by size:
5335 1481 997 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2608 3898 4738 5112 2608
Size: 1290 840 374 5405
Fragments arranged by size:
5405 1290 840 374
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7909
PsiI TTA'TAA
Cuts at: 5479 5479
Size: 7909
PspOMI G'GGCC_C
Cuts at: 2590 2590
Size: 7909
PspXI vC'TCGA_Gb
Cuts at: 3998 3998
Size: 7909
PstI C_TGCA'G
Cuts at: 830 2283 2355 2688 2820 3027 3396 3602 3906
Size: 1453 72 333 132 207 369 206 304
Cuts at: 3906 3948 4170 4287 4764 5261 830
Size: 42 222 117 477 497 3478
Fragments arranged by size:
3478 1453 497 477 369 333 304 222
207 206 132 117 72 42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7909
PvuII CAG'CTG
Cuts at: 2650 3457 3683 5058 2650
Size: 807 226 1375 5501
Fragments arranged by size:
5501 1375 807 226
SacI G_AGCT'C
Cuts at: 2244 2484 3957 4134 2244
Size: 240 1473 177 6019
Fragments arranged by size:
6019 1473 240 177
SalI G'TCGA_C
Cuts at: 1936 5251 1936
Size: 3315 4594
SbfI CC_TGCA'GG
Cuts at: 2283 2820 3906 5261 2283
Size: 537 1086 1355 4931
Fragments arranged by size:
4931 1355 1086 537
ScaI AGT'ACT
Cuts at: 5192 6157 7869 5192
Size: 965 1712 5232
Fragments arranged by size:
5232 1712 965
SfcI C'TryA_G
Cuts at: 826 1045 2279 2315 2351 2684 2816 3023 3392
Size: 219 1234 36 36 333 132 207 369
Cuts at: 3392 3598 3889 3902 3944 4166 4283 4760 5088
Size: 206 291 13 42 222 117 477 328
Cuts at: 5088 5257 6392 7073 7264 826
Size: 169 1135 681 191 1471
Fragments arranged by size:
1471 1234 1135 681 477 369 333 328
291 222 219 207 206 191 169 132
117 42 36 36 13
SfoI GGC'GCC
Cuts at: 2092 2092
Size: 7909
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7909
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7909
SmaI CCC'GGG
Cuts at: 1872 4383 4887 5236 1872
Size: 2511 504 349 4545
Fragments arranged by size:
4545 2511 504 349
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2483 2933 3152 3506 3731 3911
Size: 197 926 540 450 219 354 225 180
Cuts at: 3911 3998 4055 6013 6914 7176 820
Size: 87 57 1958 901 262 1553
Fragments arranged by size:
1958 1553 926 901 540 450 354 262
225 219 197 180 87 57
SnaBI TAC'GTA
Cuts at: 493 5206 5700 493
Size: 4713 494 2702
Fragments arranged by size:
4713 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7909
SphI G_CATG'C
Cuts at: 1132 4030 5267 1132
Size: 2898 1237 3774
Fragments arranged by size:
3774 2898 1237
SrfI GCCC'GGGC
Cuts at: 4383 4383
Size: 7909
SspI AAT'ATT
Cuts at: 5 52 5833 5
Size: 47 5781 2081
Fragments arranged by size:
5781 2081 47
StuI AGG'CCT
Cuts at: 1517 4139 1517
Size: 2622 5287
StyI C'CwwG_G
Cuts at: 513 1065 2022 2160 2265 4140 4623 5348 513
Size: 552 957 138 105 1875 483 725 3074
Fragments arranged by size:
3074 1875 957 725 552 483 138 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4851 5050 5936 5953 6106 6291 6736
Size: 1255 3271 199 886 17 153 185 445
Cuts at: 6736 325
Size: 1498
Fragments arranged by size:
3271 1498 1255 886 445 199 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5190 6155 7867
Size: 274 80 33 51 1443 3213 965 1712
Cuts at: 7867 96
Size: 138
Fragments arranged by size:
3213 1712 1443 965 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7869 7901 7869
Size: 32 7877
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7909
XbaI T'CTAG_A
Cuts at: 3802 5245 3802
Size: 1443 6466
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2473 2473
Size: 7909
XhoI C'TCGA_G
Cuts at: 1943 3998 1943
Size: 2055 5854
XmaI C'CCGG_G
Cuts at: 1870 4381 4885 5234 1870
Size: 2511 504 349 4545
Fragments arranged by size:
4545 2511 504 349
XmnI GAAnn'nnTTC
Cuts at: 2310 3249 6038 2310
Size: 939 2789 4181
Fragments arranged by size:
4181 2789 939
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5717 276
Size: 53 83 186 936 4183 2468
Fragments arranged by size:
4183 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I
Bsu36I EcoRV FseI FspAI NruI NsiI PacI PsrI
RsrII SacII SanDI SapI SexAI SfiI SwaI