Restriction map for
pFN21ASDA1267
(Circular) (Six-base) MAPSORT of: pFN21ASDA1267.seq Check: 7185 from: 1 to: 8044
pFN21ASDA1267 8044 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5854 278
Size: 53 83 186 936 4318 2468
Fragments arranged by size:
4318 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3626 5387 1937
Size: 1689 1761 4594
Fragments arranged by size:
4594 1761 1689
Acc65I G'GTAC_C
Cuts at: 3475 5365 3475
Size: 1890 6154
AclI AA'CG_TT
Cuts at: 1563 6171 6544 1563
Size: 4608 373 3063
Fragments arranged by size:
4608 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2082 2311 3093 3096 3362 3662 4691 4736
Size: 450 229 782 3 266 300 1029 45
Cuts at: 4736 6107 7491 7759 1632
Size: 1371 1384 268 1917
Fragments arranged by size:
1917 1384 1371 1029 782 450 300 268
266 229 45 3
AfeI AGC'GCT
Cuts at: 2933 4331 2933
Size: 1398 6646
AflII C'TTAA_G
Cuts at: 820 1017 4136 820
Size: 197 3119 4728
Fragments arranged by size:
4728 3119 197
AflIII A'CryG_T
Cuts at: 1203 3800 6943 7920 1203
Size: 2597 3143 977 1327
Fragments arranged by size:
3143 2597 1327 977
AgeI A'CCGG_T
Cuts at: 6875 6875
Size: 8044
AhdI GACnn_n'nnGTC
Cuts at: 6773 6773
Size: 8044
AleI CACnn'nnGTG
Cuts at: 1166 3785 4832 5033 1166
Size: 2619 1047 201 4177
Fragments arranged by size:
4177 2619 1047 201
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5224 1184
Size: 4040 4004
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5100 5132 5100
Size: 32 8012
AlwNI CAG_nnn'CTG
Cuts at: 2060 3366 4714 5787 7359 2060
Size: 1306 1348 1073 1572 2745
Fragments arranged by size:
2745 1572 1348 1306 1073
ApaLI G'TGCA_C
Cuts at: 6101 7257 6101
Size: 1156 6888
ApoI r'AATT_y
Cuts at: 1493 3408 3963 5353 5579 5731 7840 1493
Size: 1915 555 1390 226 152 2109 1697
Fragments arranged by size:
2109 1915 1697 1390 555 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6598 160
Size: 872 5566 1606
Fragments arranged by size:
5566 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8044
AvaI C'yCGr_G
Cuts at: 1870 1943 3290 5040 5369 1870
Size: 73 1347 1750 329 4545
Fragments arranged by size:
4545 1750 1347 329 73
AvrII C'CTAG_G
Cuts at: 4988 4988
Size: 8044
BamHI G'GATC_C
Cuts at: 2194 5374 5741 2194
Size: 3180 367 4497
Fragments arranged by size:
4497 3180 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2035 3475 3590 3779 5365
Size: 325 217 627 248 1440 115 189 1586
Cuts at: 5365 6820 618
Size: 1455 1842
Fragments arranged by size:
1842 1586 1455 1440 627 325 248 217
189 115
BanII G_rGCy'C
Cuts at: 1391 2569 4598 4997 5363 1391
Size: 1178 2029 399 366 4072
Fragments arranged by size:
4072 2029 1178 399 366
BbeI G_GCGC'C
Cuts at: 2039 2039
Size: 8044
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2238 2317 2826 3008 3536 928
Size: 971 339 79 509 182 528 5436
Fragments arranged by size:
5436 971 528 509 339 182 79
BbvCI CC'TCA_GC
Cuts at: 3723 3723
Size: 8044
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6235 6269 1903
Size: 34 4298 34 3678
Fragments arranged by size:
4298 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5065 5936 7146 5065
Size: 871 1210 5963
Fragments arranged by size:
5963 1210 871
BclI T'GATC_A
Cuts at: 1548 2527 1548
Size: 979 7065
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4690 6655 136
Size: 107 122 71 631 605 3018 1965 1525
Fragments arranged by size:
3018 1965 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8039 8039
Size: 8044
BlpI GC'TnA_GC
Cuts at: 2388 5319 5461 2388
Size: 2931 142 4971
Fragments arranged by size:
4971 2931 142
Bme1580I G_kGCm'C
Cuts at: 1790 2917 3595 4296 4813 6105 7261 1790
Size: 1127 678 701 517 1292 1156 2573
Fragments arranged by size:
2573 1292 1156 1127 701 678 517
BmgBI CAC'GTC
Cuts at: 1318 3803 1318
Size: 2485 5559
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3994 5775 6733
Size: 416 53 83 399 24 2577 1781 958
Cuts at: 6733 442
Size: 1753
Fragments arranged by size:
2577 1781 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8044
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2767 6704 1239
Size: 1528 3937 2579
Fragments arranged by size:
3937 2579 1528
Bpu10I CC'TnA_GC
Cuts at: 3723 3723
Size: 8044
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3055 3103 3214 5270 6169 7034 7332 3055
Size: 48 111 2056 899 865 298 3767
Fragments arranged by size:
3767 2056 899 865 298 111 48
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3031 3095 4383 6707 882
Size: 617 1532 64 1288 2324 2219
Fragments arranged by size:
2324 2219 1532 1288 617 64
BsaAI yAC'GTr
Cuts at: 493 1204 3443 5341 5835 493
Size: 711 2239 1898 494 2702
Fragments arranged by size:
2702 2239 1898 711 494
BsaBI GATnn'nnATC
Cuts at: 2611 3909 8038 2611
Size: 1298 4129 2617
Fragments arranged by size:
4129 2617 1298
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2036 5851 6233 6868
Size: 53 83 186 936 503 3815 382 635
Cuts at: 6868 275
Size: 1451
Fragments arranged by size:
3815 1451 936 635 503 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2076 3876 6477 6875 7149 7296 7707 2076
Size: 1800 2601 398 274 147 411 2413
Fragments arranged by size:
2601 2413 1800 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3940 3970 5254 5284 1178
Size: 30 165 30 2537 30 1284 30 3938
Fragments arranged by size:
3938 2537 1284 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4498 4594 4748 4811 1186
Size: 3312 96 154 63 4419
Fragments arranged by size:
4419 3312 154 96 63
BseYI C'CCAG_C
Cuts at: 2198 2983 4685 4842 4893 4897 4923 5095 7247
Size: 785 1702 157 51 4 26 172 2152
Cuts at: 7247 7743 2198
Size: 496 2499
Fragments arranged by size:
2499 2152 1702 785 496 172 157 51
26 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3220 4506 4997 5194 1163
Size: 573 1484 1286 491 197 4013
Fragments arranged by size:
4013 1484 1286 573 491 197
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4882 5494 6255 7283 1540
Size: 275 186 2881 612 761 1028 2301
Fragments arranged by size:
2881 2301 1028 761 612 275 186
BsiHKAI G_wGCw'C
Cuts at: 3646 4150 4598 6105 6190 7261 3646
Size: 504 448 1507 85 1071 4429
Fragments arranged by size:
4429 1507 1071 504 448 85
BsmI GAATG_Cn'
Cuts at: 5555 5648 5555
Size: 93 7951
BsmBI CGTCTCn'nnnn_
Cuts at: 3307 3766 3307
Size: 459 7585
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2569 2917 3595 3646 4150 4296 4598
Size: 399 779 348 678 51 504 146 302
Cuts at: 4598 4813 4997 5363 6105 6190 7261 1391
Size: 215 184 366 742 85 1071 2174
Fragments arranged by size:
2174 1071 779 742 678 504 399 366
348 302 215 184 146 85 51
BspEI T'CCGG_A
Cuts at: 7707 7707
Size: 8044
BspHI T'CATG_A
Cuts at: 2530 5933 7663 2530
Size: 3403 1730 2911
Fragments arranged by size:
3403 2911 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2947 3309 4848 5399 844
Size: 2103 362 1539 551 3489
Fragments arranged by size:
3489 2103 1539 551 362
BsrBI CCG'CTC
Cuts at: 1394 1823 2042 5131 5931 1394
Size: 429 219 3089 800 3507
Fragments arranged by size:
3507 3089 800 429 219
BsrDI GCAATG_nn'
Cuts at: 65 1248 2181 6539 6713 65
Size: 1183 933 4358 174 1396
Fragments arranged by size:
4358 1396 1183 933 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2740 6688 6875 1700
Size: 110 930 3948 187 2869
Fragments arranged by size:
3948 2869 930 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8044
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8044
BssSI C'ACGA_G
Cuts at: 4828 6104 7116 4828
Size: 1276 1012 5756
Fragments arranged by size:
5756 1276 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8044
BstBI TT'CG_AA
Cuts at: 2007 6848 2007
Size: 4841 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8044
BstYI r'GATC_y
Cuts at: 1972 2194 2606 5374 5741 6126 6143 7595 7681
Size: 222 412 2768 367 385 17 1452 86
Cuts at: 7681 7693 8039 1972
Size: 12 346 1977
Fragments arranged by size:
2768 1977 1452 412 385 367 346 222
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5224 5224
Size: 8044
BtgI C'CryG_G
Cuts at: 513 1065 2022 2730 3041 3586 4296 4975 513
Size: 552 957 708 311 545 710 679 3582
Fragments arranged by size:
3582 957 710 708 679 552 545 311
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2045 4545 487
Size: 683 56 819 2500 3986
Fragments arranged by size:
3986 2500 819 683 56
BtsI GCAGTG_nn'
Cuts at: 2125 2703 3208 4909 5560 6354 6374 2125
Size: 578 505 1701 651 794 20 3795
Fragments arranged by size:
3795 1701 794 651 578 505 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8044
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3619 3654 529
Size: 35 3055 35 4919
Fragments arranged by size:
4919 3055 35 35
DraI TTT'AAA
Cuts at: 5348 5348
Size: 8044
DraIII CAC_nnn'GTG
Cuts at: 3929 3929
Size: 8044
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7051 809
Size: 507 5735 1802
Fragments arranged by size:
5735 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4879 5239 5491
Size: 54 1425 50 275 18 3049 360 252
Cuts at: 5491 6380 8009 8
Size: 889 1629 43
Fragments arranged by size:
3049 1629 1425 889 360 275 252 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4879 5491 1537
Size: 275 3067 612 4090
Fragments arranged by size:
4090 3067 612 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2589 2655 3258 3681 3769 4737 5974 1353
Size: 1236 66 603 423 88 968 1237 3423
Fragments arranged by size:
3423 1237 1236 968 603 423 88 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3881 5149 5180 6631 7005 7151
Size: 1064 138 2292 1268 31 1451 374 146
Cuts at: 7151 387
Size: 1280
Fragments arranged by size:
2292 1451 1280 1268 1064 374 146 138
31
EcoICRI GAG'CTC
Cuts at: 4596 4596
Size: 8044
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2082 2311 2767 3093 3096 3362 3662
Size: 393 450 229 456 326 3 266 300
Cuts at: 3662 4691 4736 6107 6704 7491 7759 1239
Size: 1029 45 1371 597 787 268 1524
Fragments arranged by size:
1524 1371 1029 787 597 456 450 393
326 300 268 266 229 45 3
EcoO109I rG'GnC_Cy
Cuts at: 4257 4753 4860 4257
Size: 496 107 7441
Fragments arranged by size:
7441 496 107
EcoRI G'AATT_C
Cuts at: 3408 5353 5731 3408
Size: 1945 378 5721
Fragments arranged by size:
5721 1945 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2251 2251
Size: 8044
FspI TGC'GCA
Cuts at: 3922 6550 3922
Size: 2628 5416
HaeII r_GCGC'y
Cuts at: 1678 2039 2935 4319 4333 5261 5326 7191 1678
Size: 361 896 1384 14 928 65 1865 2531
Fragments arranged by size:
2531 1865 1384 928 896 361 65 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2270 2302 2771 2803 3046
Size: 32 135 32 698 32 469 32 243
Cuts at: 3046 3078 3608 3640 3741 3773 4382 4414 4694
Size: 32 530 32 101 32 609 32 280
Cuts at: 4694 4726 4742 4774 5096 5128 5281 5313 6685
Size: 32 16 32 322 32 153 32 1372
Cuts at: 6685 6717 6759 6791 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1372 698 609 530 469 322 280
243 153 135 101 42 32 32 32
32 32 32 32 32 32 32 32
32 32 32 16
HincII GTy'rAC
Cuts at: 669 1657 1938 5388 5634 669
Size: 988 281 3450 246 3079
Fragments arranged by size:
3450 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8044
HpaI GTT'AAC
Cuts at: 5634 5634
Size: 8044
KasI G'GCGC_C
Cuts at: 2035 2035
Size: 8044
KpnI G_GTAC'C
Cuts at: 3479 5369 3479
Size: 1890 6154
MfeI C'AATT_G
Cuts at: 2641 5643 2641
Size: 3002 5042
MluI A'CGCG_T
Cuts at: 7920 7920
Size: 8044
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3966 3984 4785 7158 7342 1305
Size: 2661 18 801 2373 184 2007
Fragments arranged by size:
2661 2373 2007 801 184 18
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6222
Fragments arranged by size:
6222 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2592 3785 4433 4625 4832 5033 6002
Size: 648 1426 1193 648 192 207 201 969
Cuts at: 6002 6361 6520 518
Size: 359 159 2042
Fragments arranged by size:
2042 1426 1193 969 648 648 359 207
201 192 159
MspA1I CmG'CkG
Cuts at: 1606 2060 2983 3366 4689 4846 4897 5182 5208
Size: 454 923 383 1323 157 51 285 26
Cuts at: 5208 5460 6137 7285 7530 1606
Size: 252 677 1148 245 2120
Fragments arranged by size:
2120 1323 1148 923 677 454 383 285
252 245 157 51 26
NaeI GCC'GGC
Cuts at: 2742 2742
Size: 8044
NarI GG'CG_CC
Cuts at: 2036 2036
Size: 8044
NcoI C'CATG_G
Cuts at: 513 1065 2022 2730 3586 4296 4975 513
Size: 552 957 708 856 710 679 3582
Fragments arranged by size:
3582 957 856 710 708 679 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8044
NgoMIV G'CCGG_C
Cuts at: 2740 2740
Size: 8044
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8044
NotI GC'GGCC_GC
Cuts at: 5491 5491
Size: 8044
NspI r_CATG'y
Cuts at: 1132 1229 4075 4787 5402 6947 1132
Size: 97 2846 712 615 1545 2229
Fragments arranged by size:
2846 2229 1545 712 615 97
PasI CC'CwG_GG
Cuts at: 1348 5124 1348
Size: 3776 4268
PciI A'CATG_T
Cuts at: 6943 6943
Size: 8044
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8044
PmeI GTTT'AAAC
Cuts at: 5348 5348
Size: 8044
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6113 6145 7626 7658 6113
Size: 32 1481 32 6499
Fragments arranged by size:
6499 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4257 4753 4860 4257
Size: 496 107 7441
Fragments arranged by size:
7441 496 107
PshAI GACnn'nnGTC
Cuts at: 1613 3319 1613
Size: 1706 6338
PsiI TTA'TAA
Cuts at: 5614 5614
Size: 8044
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2274 2306 2431 2463 2274
Size: 32 125 32 7855
Fragments arranged by size:
7855 125 32 32
PstI C_TGCA'G
Cuts at: 830 2191 3343 5396 830
Size: 1361 1152 2053 3478
Fragments arranged by size:
3478 2053 1361 1152
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8044
PvuII CAG'CTG
Cuts at: 2060 2983 4689 4897 5182 2060
Size: 923 1706 208 285 4922
Fragments arranged by size:
4922 1706 923 285 208
SacI G_AGCT'C
Cuts at: 4598 4598
Size: 8044
SalI G'TCGA_C
Cuts at: 1936 5386 1936
Size: 3450 4594
SapI GCTCTTCn'nnn_
Cuts at: 2589 2589
Size: 8044
SbfI CC_TGCA'GG
Cuts at: 5396 5396
Size: 8044
ScaI AGT'ACT
Cuts at: 6292 8004 6292
Size: 1712 6332
SexAI A'CCwGG_T
Cuts at: 2770 2770
Size: 8044
SfcI C'TryA_G
Cuts at: 826 1045 2187 3339 4864 5392 6527 7208 7399
Size: 219 1142 1152 1525 528 1135 681 191
Cuts at: 7399 826
Size: 1471
Fragments arranged by size:
1525 1471 1152 1142 1135 681 528 219
191
SfoI GGC'GCC
Cuts at: 2037 2037
Size: 8044
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8044
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8044
SmaI CCC'GGG
Cuts at: 1872 5371 1872
Size: 3499 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3034 3082 3193 4136 5285 6148
Size: 197 926 1091 48 111 943 1149 863
Cuts at: 6148 7049 7311 820
Size: 901 262 1553
Fragments arranged by size:
1553 1149 1091 943 926 901 863 262
197 111 48
SnaBI TAC'GTA
Cuts at: 493 5341 5835 493
Size: 4848 494 2702
Fragments arranged by size:
4848 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8044
SphI G_CATG'C
Cuts at: 1132 4075 4787 5402 1132
Size: 2943 712 615 3774
Fragments arranged by size:
3774 2943 712 615
SspI AAT'ATT
Cuts at: 5 52 5968 5
Size: 47 5916 2081
Fragments arranged by size:
5916 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8044
StyI C'CwwG_G
Cuts at: 513 1065 2022 2226 2730 3586 4296 4485 4975
Size: 552 957 204 504 856 710 189 490
Cuts at: 4975 4988 5483 513
Size: 13 495 3074
Fragments arranged by size:
3074 957 856 710 552 504 495 490
204 189 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2975 4165 4478 6071 6088 6241 6426
Size: 1255 1395 1190 313 1593 17 153 185
Cuts at: 6426 6871 325
Size: 445 1498
Fragments arranged by size:
1593 1498 1395 1255 1190 445 313 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3808 6290 8002
Size: 274 80 33 51 1443 1831 2482 1712
Cuts at: 8002 96
Size: 138
Fragments arranged by size:
2482 1831 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2058 2090 3940 3972 4453 4485 4816 4848 5254
Size: 32 1850 32 481 32 331 32 406
Cuts at: 5254 5286 8004 8036 2058
Size: 32 2718 32 2066
Fragments arranged by size:
2718 2066 1850 481 406 331 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8044
XbaI T'CTAG_A
Cuts at: 5380 5380
Size: 8044
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 2233 2791 5092 2029
Size: 204 558 2301 4981
Fragments arranged by size:
4981 2301 558 204
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8044
XmaI C'CCGG_G
Cuts at: 1870 5369 1870
Size: 3499 4545
XmnI GAAnn'nnTTC
Cuts at: 6173 6173
Size: 8044
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5852 276
Size: 53 83 186 936 4318 2468
Fragments arranged by size:
4318 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI BaeI BfrBI BplI BsiWI BstXI
BstZ17I EcoNI EcoRV FseI FspAI NruI NsiI PacI
PfoI PmlI PspOMI PspXI RsrII SacII SanDI SfiI
SrfI SwaI