Restriction map for
pFN21ASDA1266
(Circular) (Six-base) MAPSORT of: pFN21ASDA1266.seq Check: 5598 from: 1 to: 6340
pFN21ASDA1266 6340 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4150 278
Size: 53 83 186 936 2614 2468
Fragments arranged by size:
2614 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3683 1937
Size: 1746 4594
Acc65I G'GTAC_C
Cuts at: 3661 3661
Size: 6340
AclI AA'CG_TT
Cuts at: 1563 4467 4840 1563
Size: 2904 373 3063
Fragments arranged by size:
3063 2904 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2697 4403 5787 6055 1632
Size: 1065 1706 1384 268 1917
Fragments arranged by size:
1917 1706 1384 1065 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6143
AflIII A'CryG_T
Cuts at: 1203 2787 3174 5239 6216 1203
Size: 1584 387 2065 977 1327
Fragments arranged by size:
2065 1584 1327 977 387
AgeI A'CCGG_T
Cuts at: 5171 5171
Size: 6340
AhdI GACnn_n'nnGTC
Cuts at: 5069 5069
Size: 6340
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6340
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2541 1184
Size: 1357 4983
AlwNI CAG_nnn'CTG
Cuts at: 2675 2843 4083 5655 2675
Size: 168 1240 1572 3360
Fragments arranged by size:
3360 1572 1240 168
ApaI G_GGCC'C
Cuts at: 3035 3035
Size: 6340
ApaLI G'TGCA_C
Cuts at: 4397 5553 4397
Size: 1156 5184
ApoI r'AATT_y
Cuts at: 1493 3281 3649 3875 4027 6136 1493
Size: 1788 368 226 152 2109 1697
Fragments arranged by size:
2109 1788 1697 368 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3351 4894 160
Size: 872 2319 1543 1606
Fragments arranged by size:
2319 1606 1543 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6340
AvaI C'yCGr_G
Cuts at: 1870 1943 2541 3665 1870
Size: 73 598 1124 4545
Fragments arranged by size:
4545 1124 598 73
BamHI G'GATC_C
Cuts at: 2401 3670 4037 2401
Size: 1269 367 4704
Fragments arranged by size:
4704 1269 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3661 5116 618
Size: 325 217 627 1874 1455 1842
Fragments arranged by size:
1874 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2482 3035 3659 1391
Size: 1091 553 624 4072
Fragments arranged by size:
4072 1091 624 553
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3245 928
Size: 971 1346 4023
Fragments arranged by size:
4023 1346 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4531 4565 1903
Size: 34 2594 34 3678
Fragments arranged by size:
3678 2594 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2230 2563 4232 5442 2230
Size: 333 1669 1210 3128
Fragments arranged by size:
3128 1669 1210 333
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6340
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3368 4951 136
Size: 107 122 71 631 605 1696 1583 1525
Fragments arranged by size:
1696 1583 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6335 6335
Size: 6340
BlpI GC'TnA_GC
Cuts at: 3757 3757
Size: 6340
Bme1580I G_kGCm'C
Cuts at: 1790 3035 4401 5557 1790
Size: 1245 1366 1156 2573
Fragments arranged by size:
2573 1366 1245 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6340
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3106 3225 4071
Size: 416 53 83 399 24 1689 119 846
Cuts at: 4071 5029 442
Size: 958 1753
Fragments arranged by size:
1753 1689 958 846 416 399 119 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2685 1056
Size: 1629 4711
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5000 1239
Size: 3761 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4465 5330 5628 4465
Size: 865 298 5177
Fragments arranged by size:
5177 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5003 882
Size: 617 3504 2219
Fragments arranged by size:
3504 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2456 3175 3618 3637 4131 493
Size: 711 1252 719 443 19 494 2702
Fragments arranged by size:
2702 1252 719 711 494 443 19
BsaBI GATnn'nnATC
Cuts at: 6334 6334
Size: 6340
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4147 4529 5164 275
Size: 53 83 186 936 2614 382 635 1451
Fragments arranged by size:
2614 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4773 5171 5445 5592 6003 4773
Size: 398 274 147 411 5110
Fragments arranged by size:
5110 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2622 2651 2652 2681 2971
Size: 30 165 30 1219 29 1 29 290
Cuts at: 2971 3001 1178
Size: 30 4517
Fragments arranged by size:
4517 1219 290 165 30 30 30 29
29 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6340
BseYI C'CCAG_C
Cuts at: 5543 6039 5543
Size: 496 5844
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2200 1163
Size: 573 464 5303
Fragments arranged by size:
5303 573 464
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3338 3790 4551 5579 1540
Size: 275 186 1337 452 761 1028 2301
Fragments arranged by size:
2301 1337 1028 761 452 275 186
BsiHKAI G_wGCw'C
Cuts at: 4401 4486 5557 4401
Size: 85 1071 5184
Fragments arranged by size:
5184 1071 85
BsmI GAATG_Cn'
Cuts at: 3204 3567 3851 3944 3204
Size: 363 284 93 5600
Fragments arranged by size:
5600 363 284 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2482 3035 3659 4401 4486 5557 1391
Size: 399 692 553 624 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 692 624 553 399 85
BspEI T'CCGG_A
Cuts at: 6003 6003
Size: 6340
BspHI T'CATG_A
Cuts at: 4229 5959 4229
Size: 1730 4610
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3695 844
Size: 2851 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4227 1394
Size: 429 2404 3507
Fragments arranged by size:
3507 2404 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4835 5009 65
Size: 1183 3587 174 1396
Fragments arranged by size:
3587 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4984 5171 1700
Size: 110 3174 187 2869
Fragments arranged by size:
3174 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6340
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6340
BssSI C'ACGA_G
Cuts at: 3159 4400 5412 3159
Size: 1241 1012 4087
Fragments arranged by size:
4087 1241 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3199 3377 794
Size: 2405 178 3757
Fragments arranged by size:
3757 2405 178
BstBI TT'CG_AA
Cuts at: 2007 5144 2007
Size: 3137 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6340
BstXI CCAn_nnnn'nTGG
Cuts at: 2482 2482
Size: 6340
BstYI r'GATC_y
Cuts at: 1972 2401 3465 3670 4037 4422 4439 5891 5977
Size: 429 1064 205 367 385 17 1452 86
Cuts at: 5977 5989 6335 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1064 429 385 367 346 205
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5788
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5601
Fragments arranged by size:
5601 683 56
BtsI GCAGTG_nn'
Cuts at: 2188 2608 3053 3240 3856 4650 4670 2188
Size: 420 445 187 616 794 20 3858
Fragments arranged by size:
3858 794 616 445 420 187 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6340
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6305
DraI TTT'AAA
Cuts at: 3644 3644
Size: 6340
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5347 809
Size: 507 4031 1802
Fragments arranged by size:
4031 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3335 3787 4676
Size: 54 1425 50 275 18 1505 452 889
Cuts at: 4676 6305 8
Size: 1629 43
Fragments arranged by size:
1629 1505 1425 889 452 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3335 3787 1537
Size: 275 1523 452 4090
Fragments arranged by size:
4090 1523 452 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2688 3117 4270 1353
Size: 1335 429 1153 3423
Fragments arranged by size:
3423 1335 1153 429
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4927 5301 5447 387
Size: 1064 138 3338 374 146 1280
Fragments arranged by size:
3338 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2697 4403 5000 5787 6055 1239
Size: 393 1065 1706 597 787 268 1524
Fragments arranged by size:
1706 1524 1065 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2973 2973
Size: 6340
EcoO109I rG'GnC_Cy
Cuts at: 2312 2633 3031 3154 2312
Size: 321 398 123 5498
Fragments arranged by size:
5498 398 321 123
EcoRI G'AATT_C
Cuts at: 3649 4027 3649
Size: 378 5962
EcoRV GAT'ATC
Cuts at: 2321 2321
Size: 6340
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2762 2762
Size: 6340
FspI TGC'GCA
Cuts at: 4846 4846
Size: 6340
HaeII r_GCGC'y
Cuts at: 1678 5487 1678
Size: 3809 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2045 2077 2192 2224 2651
Size: 32 135 32 473 32 115 32 427
Cuts at: 2651 2683 3116 3148 4981 5013 5055 5087 1373
Size: 32 433 32 1833 32 42 32 2626
Fragments arranged by size:
2626 1833 473 433 427 135 115 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3684 3930 669
Size: 988 281 1746 246 3079
Fragments arranged by size:
3079 1746 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3249 748
Size: 2501 3839
HpaI GTT'AAC
Cuts at: 3930 3930
Size: 6340
KpnI G_GTAC'C
Cuts at: 3665 3665
Size: 6340
MfeI C'AATT_G
Cuts at: 3939 3939
Size: 6340
MluI A'CGCG_T
Cuts at: 6216 6216
Size: 6340
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5454 5638 1305
Size: 4149 184 2007
Fragments arranged by size:
4149 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4518
Fragments arranged by size:
4518 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2523 2589 3597 4298 4657 4816 518
Size: 648 1357 66 1008 701 359 159 2042
Fragments arranged by size:
2042 1357 1008 701 648 359 159 66
MspA1I CmG'CkG
Cuts at: 1606 3756 4433 5581 5826 1606
Size: 2150 677 1148 245 2120
Fragments arranged by size:
2150 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5788
NdeI CA'TA_TG
Cuts at: 387 2520 387
Size: 2133 4207
NheI G'CTAG_C
Cuts at: 1052 2681 1052
Size: 1629 4711
NotI GC'GGCC_GC
Cuts at: 3787 3787
Size: 6340
NspI r_CATG'y
Cuts at: 1132 1229 2042 2791 3392 3447 3698 5243 1132
Size: 97 813 749 601 55 251 1545 2229
Fragments arranged by size:
2229 1545 813 749 601 251 97 55
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6340
PciI A'CATG_T
Cuts at: 2787 5239 2787
Size: 2452 3888
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6340
PmeI GTTT'AAAC
Cuts at: 3644 3644
Size: 6340
PmlI CAC'GTG
Cuts at: 2456 3175 3618 2456
Size: 719 443 5178
Fragments arranged by size:
5178 719 443
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4409 4441 5922 5954 4409
Size: 32 1481 32 4795
Fragments arranged by size:
4795 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2312 2633 3154 2312
Size: 321 521 5498
Fragments arranged by size:
5498 521 321
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6340
PsiI TTA'TAA
Cuts at: 3910 3910
Size: 6340
PspOMI G'GGCC_C
Cuts at: 3031 3031
Size: 6340
PstI C_TGCA'G
Cuts at: 830 2536 3235 3692 830
Size: 1706 699 457 3478
Fragments arranged by size:
3478 1706 699 457
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6340
SalI G'TCGA_C
Cuts at: 1936 3682 1936
Size: 1746 4594
SbfI CC_TGCA'GG
Cuts at: 3692 3692
Size: 6340
ScaI AGT'ACT
Cuts at: 4588 6300 4588
Size: 1712 4628
SfcI C'TryA_G
Cuts at: 826 1045 2532 3147 3231 3586 3688 4823 5504
Size: 219 1487 615 84 355 102 1135 681
Cuts at: 5504 5695 826
Size: 191 1471
Fragments arranged by size:
1487 1471 1135 681 615 355 219 191
102 84
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6340
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6340
SmaI CCC'GGG
Cuts at: 1872 3667 1872
Size: 1795 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4444 5345 5607 820
Size: 197 926 2501 901 262 1553
Fragments arranged by size:
2501 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3637 4131 493
Size: 3144 494 2702
Fragments arranged by size:
3144 2702 494
SpeI A'CTAG_T
Cuts at: 152 2906 152
Size: 2754 3586
SphI G_CATG'C
Cuts at: 1132 2042 3698 1132
Size: 910 1656 3774
Fragments arranged by size:
3774 1656 910
SspI AAT'ATT
Cuts at: 5 52 2780 4264 5
Size: 47 2728 1484 2081
Fragments arranged by size:
2728 2081 1484 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6340
StyI C'CwwG_G
Cuts at: 513 1065 2750 3779 513
Size: 552 1685 1029 3074
Fragments arranged by size:
3074 1685 1029 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2105 3125 4367 4384 4537 4722 5167
Size: 1255 525 1020 1242 17 153 185 445
Cuts at: 5167 325
Size: 1498
Fragments arranged by size:
1498 1255 1242 1020 525 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4586 6298 96
Size: 274 80 33 51 1443 2609 1712 138
Fragments arranged by size:
2609 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2908 2940 2971 3003 3205 3237 6300 6332 2908
Size: 32 31 32 202 32 3063 32 2916
Fragments arranged by size:
3063 2916 202 32 32 32 32 31
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6340
XbaI T'CTAG_A
Cuts at: 3676 3676
Size: 6340
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6340
XmaI C'CCGG_G
Cuts at: 1870 3665 1870
Size: 1795 4545
XmnI GAAnn'nnTTC
Cuts at: 4469 4469
Size: 6340
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4148 276
Size: 53 83 186 936 2614 2468
Fragments arranged by size:
2614 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI