Restriction map for
pFN21ASDA1264
(Circular) (Six-base) MAPSORT of: pFN21ASDA1264.seq Check: 5119 from: 1 to: 6886
pFN21ASDA1264 6886 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4696 278
Size: 53 83 186 936 3160 2468
Fragments arranged by size:
3160 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4229 1937
Size: 2292 4594
Acc65I G'GTAC_C
Cuts at: 3150 4207 3150
Size: 1057 5829
AclI AA'CG_TT
Cuts at: 1563 5013 5386 1563
Size: 3450 373 3063
Fragments arranged by size:
3450 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4017 4949 6333 6601 1632
Size: 2385 932 1384 268 1917
Fragments arranged by size:
2385 1917 1384 932 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6689
AflIII A'CryG_T
Cuts at: 1203 3546 5785 6762 1203
Size: 2343 2239 977 1327
Fragments arranged by size:
2343 2239 1327 977
AgeI A'CCGG_T
Cuts at: 5717 5717
Size: 6886
AhdI GACnn_n'nnGTC
Cuts at: 3901 5615 3901
Size: 1714 5172
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6886
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6886
AlwNI CAG_nnn'CTG
Cuts at: 3062 4629 6201 3062
Size: 1567 1572 3747
Fragments arranged by size:
3747 1572 1567
ApaI G_GGCC'C
Cuts at: 3083 3083
Size: 6886
ApaLI G'TGCA_C
Cuts at: 4943 6099 4943
Size: 1156 5730
ApoI r'AATT_y
Cuts at: 1493 4195 4421 4573 6682 1493
Size: 2702 226 152 2109 1697
Fragments arranged by size:
2702 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5440 160
Size: 872 4408 1606
Fragments arranged by size:
4408 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6886
AvaI C'yCGr_G
Cuts at: 1870 1943 2689 2890 3908 4211 1870
Size: 73 746 201 1018 303 4545
Fragments arranged by size:
4545 1018 746 303 201 73
BamHI G'GATC_C
Cuts at: 2491 3984 4216 4583 2491
Size: 1493 232 367 4794
Fragments arranged by size:
4794 1493 367 232
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3150 3608 3811 4207 5662
Size: 325 217 627 1363 458 203 396 1455
Cuts at: 5662 618
Size: 1842
Fragments arranged by size:
1842 1455 1363 627 458 396 325 217
203
BanII G_rGCy'C
Cuts at: 1391 2994 3083 3230 3275 3579 3688 3945 4077
Size: 1603 89 147 45 304 109 257 132
Cuts at: 4077 4205 1391
Size: 128 4072
Fragments arranged by size:
4072 1603 304 257 147 132 128 109
89 45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2231 2823 928
Size: 971 332 592 4991
Fragments arranged by size:
4991 971 592 332
BbvCI CC'TCA_GC
Cuts at: 2856 3041 3233 2856
Size: 185 192 6509
Fragments arranged by size:
6509 192 185
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2417 2451 5077 5111 1903
Size: 34 480 34 2626 34 3678
Fragments arranged by size:
3678 2626 480 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4778 5988 4778
Size: 1210 5676
BclI T'GATC_A
Cuts at: 1548 3510 1548
Size: 1962 4924
BfrBI ATG'CAT
Cuts at: 2196 3414 2196
Size: 1218 5668
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5497 136
Size: 107 122 71 631 605 3825 1525
Fragments arranged by size:
3825 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6881 6881
Size: 6886
BlpI GC'TnA_GC
Cuts at: 4303 4303
Size: 6886
Bme1580I G_kGCm'C
Cuts at: 1790 2535 3083 4947 6103 1790
Size: 745 548 1864 1156 2573
Fragments arranged by size:
2573 1864 1156 745 548
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6886
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2100 2404 4617
Size: 416 53 83 399 24 683 304 2213
Cuts at: 4617 5575 442
Size: 958 1753
Fragments arranged by size:
2213 1753 958 683 416 399 304 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6886
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2344 2882 3280 3953 5546 1239
Size: 1105 538 398 673 1593 2579
Fragments arranged by size:
2579 1593 1105 673 538 398
Bpu10I CC'TnA_GC
Cuts at: 2856 3041 3224 3233 2856
Size: 185 183 9 6509
Fragments arranged by size:
6509 185 183 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2651 3109 3780 5011 5876 6174 2651
Size: 458 671 1231 865 298 3363
Fragments arranged by size:
3363 1231 865 671 458 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3345 5549 882
Size: 617 1846 2204 2219
Fragments arranged by size:
2219 2204 1846 617
BsaAI yAC'GTr
Cuts at: 493 1204 2536 4183 4677 493
Size: 711 1332 1647 494 2702
Fragments arranged by size:
2702 1647 1332 711 494
BsaBI GATnn'nnATC
Cuts at: 2490 3021 6880 2490
Size: 531 3859 2496
Fragments arranged by size:
3859 2496 531
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4693 5075 5710 275
Size: 53 83 186 936 3160 382 635 1451
Fragments arranged by size:
3160 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3468 5319 5717 5991 6138 6549 3468
Size: 1851 398 274 147 411 3805
Fragments arranged by size:
3805 1851 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3212 3242 1178
Size: 30 165 30 1809 30 4822
Fragments arranged by size:
4822 1809 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3265 3268 4094 1186
Size: 2079 3 826 3978
Fragments arranged by size:
3978 2079 826 3
BseYI C'CCAG_C
Cuts at: 2130 2798 3263 3267 6089 6585 2130
Size: 668 465 4 2822 496 2431
Fragments arranged by size:
2822 2431 668 496 465 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6313
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2451 3025 4336 5097 6125 1540
Size: 275 186 450 574 1311 761 1028 2301
Fragments arranged by size:
2301 1311 1028 761 574 450 275 186
BsiHKAI G_wGCw'C
Cuts at: 2759 3031 3230 3579 3688 3796 4041 4947 5032
Size: 272 199 349 109 108 245 906 85
Cuts at: 5032 6103 2759
Size: 1071 3542
Fragments arranged by size:
3542 1071 906 349 272 245 199 109
108 85
BsmI GAATG_Cn'
Cuts at: 3003 4397 4490 3003
Size: 1394 93 5399
Fragments arranged by size:
5399 1394 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3475 3475
Size: 6886
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2535 2759 2994 3031 3083 3230 3275
Size: 399 745 224 235 37 52 147 45
Cuts at: 3275 3579 3688 3796 3945 4041 4077 4205 4947
Size: 304 109 108 149 96 36 128 742
Cuts at: 4947 5032 6103 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 745 742 399 304 235 224
149 147 128 109 108 96 85 52
45 37 36
BspEI T'CCGG_A
Cuts at: 3468 6549 3468
Size: 3081 3805
BspHI T'CATG_A
Cuts at: 4158 4775 6505 4158
Size: 617 1730 4539
Fragments arranged by size:
4539 1730 617
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4121 4241 844
Size: 3277 120 3489
Fragments arranged by size:
3489 3277 120
BsrBI CCG'CTC
Cuts at: 1394 1823 4773 1394
Size: 429 2950 3507
Fragments arranged by size:
3507 2950 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5381 5555 65
Size: 1183 4133 174 1396
Fragments arranged by size:
4133 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2120 3401 5530 5717 1700
Size: 110 310 1281 2129 187 2869
Fragments arranged by size:
2869 2129 1281 310 187 110
BsrGI T'GTAC_A
Cuts at: 96 3549 96
Size: 3453 3433
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6886
BssSI C'ACGA_G
Cuts at: 4946 5958 4946
Size: 1012 5874
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3411 794
Size: 2617 4269
BstBI TT'CG_AA
Cuts at: 2007 5690 2007
Size: 3683 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6886
BstXI CCAn_nnnn'nTGG
Cuts at: 2212 2212
Size: 6886
BstYI r'GATC_y
Cuts at: 1972 2491 3984 4216 4583 4968 4985 6437 6523
Size: 519 1493 232 367 385 17 1452 86
Cuts at: 6523 6535 6881 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1493 1452 519 385 367 346 232
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2321 3858 2321
Size: 1537 5349
BtgI C'CryG_G
Cuts at: 513 1065 2365 3210 513
Size: 552 1300 845 4189
Fragments arranged by size:
4189 1300 845 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3400 3585 3771 3989 487
Size: 683 56 2174 185 186 218 3384
Fragments arranged by size:
3384 2174 683 218 186 185 56
BtsI GCAGTG_nn'
Cuts at: 2141 3456 4402 5196 5216 2141
Size: 1315 946 794 20 3811
Fragments arranged by size:
3811 1315 946 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6886
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6851
DraI TTT'AAA
Cuts at: 2935 4190 2935
Size: 1255 5631
DraIII CAC_nnn'GTG
Cuts at: 3000 3000
Size: 6886
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5893 809
Size: 507 4577 1802
Fragments arranged by size:
4577 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2160 3369 3760
Size: 54 1425 50 275 18 330 1209 391
Cuts at: 3760 4333 5222 6851 8
Size: 573 889 1629 43
Fragments arranged by size:
1629 1425 1209 889 573 391 330 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4333 1537
Size: 275 2521 4090
Fragments arranged by size:
4090 2521 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2462 3812 4816 1353
Size: 1109 1350 1004 3423
Fragments arranged by size:
3423 1350 1109 1004
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5473 5847 5993 387
Size: 1064 138 3884 374 146 1280
Fragments arranged by size:
3884 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3228 3577 3686 3228
Size: 349 109 6428
Fragments arranged by size:
6428 349 109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2344 2882 3280 3953 4017 4949 5546
Size: 393 712 538 398 673 64 932 597
Cuts at: 5546 6333 6601 1239
Size: 787 268 1524
Fragments arranged by size:
1524 932 787 712 673 597 538 398
393 268 64
EcoNI CCTnn'n_nnAGG
Cuts at: 2279 3073 4022 2279
Size: 794 949 5143
Fragments arranged by size:
5143 949 794
EcoO109I rG'GnC_Cy
Cuts at: 2795 3079 3080 4015 2795
Size: 284 1 935 5666
Fragments arranged by size:
5666 935 284 1
EcoRI G'AATT_C
Cuts at: 4195 4573 4195
Size: 378 6508
FspI TGC'GCA
Cuts at: 5392 5392
Size: 6886
HaeII r_GCGC'y
Cuts at: 1678 6033 1678
Size: 4355 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2282 2314 2606 2638 2839
Size: 32 135 32 710 32 292 32 201
Cuts at: 2839 2871 3887 3919 5527 5559 5601 5633 1373
Size: 32 1016 32 1608 32 42 32 2626
Fragments arranged by size:
2626 1608 1016 710 292 201 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3544 4230 4476 669
Size: 988 281 1606 686 246 3079
Fragments arranged by size:
3079 1606 988 686 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6886
HpaI GTT'AAC
Cuts at: 4476 4476
Size: 6886
KpnI G_GTAC'C
Cuts at: 3154 4211 3154
Size: 1057 5829
MfeI C'AATT_G
Cuts at: 4485 4485
Size: 6886
MluI A'CGCG_T
Cuts at: 6762 6762
Size: 6886
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2091 2337 3306 6000 6184 1305
Size: 786 246 969 2694 184 2007
Fragments arranged by size:
2694 2007 969 786 246 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3371 10
Size: 54 1425 343 1539 3525
Fragments arranged by size:
3525 1539 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2300 3534 3638 4844 5203 5362 518
Size: 648 1134 1234 104 1206 359 159 2042
Fragments arranged by size:
2042 1234 1206 1134 648 359 159 104
MspA1I CmG'CkG
Cuts at: 1606 2212 2751 3267 3334 4302 4979 6127 6372
Size: 606 539 516 67 968 677 1148 245
Cuts at: 6372 1606
Size: 2120
Fragments arranged by size:
2120 1148 968 677 606 539 516 245
67
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6334
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6886
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6886
NotI GC'GGCC_GC
Cuts at: 4333 4333
Size: 6886
NsiI A_TGCA'T
Cuts at: 2198 3416 2198
Size: 1218 5668
NspI r_CATG'y
Cuts at: 1132 1229 3550 4244 5789 1132
Size: 97 2321 694 1545 2229
Fragments arranged by size:
2321 2229 1545 694 97
PasI CC'CwG_GG
Cuts at: 1348 3075 3357 1348
Size: 1727 282 4877
Fragments arranged by size:
4877 1727 282
PciI A'CATG_T
Cuts at: 3546 5785 3546
Size: 2239 4647
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6886
PfoI T'CCnGG_A
Cuts at: 2898 3987 2898
Size: 1089 5797
PmeI GTTT'AAAC
Cuts at: 4190 4190
Size: 6886
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4955 4987 6468 6500 4955
Size: 32 1481 32 5341
Fragments arranged by size:
5341 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2795 4015 2795
Size: 1220 5666
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6886
PsiI TTA'TAA
Cuts at: 4456 4456
Size: 6886
PspOMI G'GGCC_C
Cuts at: 3079 3079
Size: 6886
PspXI vC'TCGA_Gb
Cuts at: 2689 2689
Size: 6886
PstI C_TGCA'G
Cuts at: 830 2743 3189 3333 4238 830
Size: 1913 446 144 905 3478
Fragments arranged by size:
3478 1913 905 446 144
PvuI CG_AT'CG
Cuts at: 2001 3025 2001
Size: 1024 5862
PvuII CAG'CTG
Cuts at: 2212 3267 3334 2212
Size: 1055 67 5764
Fragments arranged by size:
5764 1055 67
SacI G_AGCT'C
Cuts at: 3230 3579 3688 3230
Size: 349 109 6428
Fragments arranged by size:
6428 349 109
SalI G'TCGA_C
Cuts at: 1936 4228 1936
Size: 2292 4594
SanDI GG'GwC_CC
Cuts at: 2795 2795
Size: 6886
SbfI CC_TGCA'GG
Cuts at: 4238 4238
Size: 6886
ScaI AGT'ACT
Cuts at: 5134 6846 5134
Size: 1712 5174
SfcI C'TryA_G
Cuts at: 826 1045 2739 3185 3329 4234 5369 6050 6241
Size: 219 1694 446 144 905 1135 681 191
Cuts at: 6241 826
Size: 1471
Fragments arranged by size:
1694 1471 1135 905 681 446 219 191
144
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6886
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6886
SmaI CCC'GGG
Cuts at: 1872 4213 1872
Size: 2341 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2666 2689 3124 3795 4990 5891
Size: 197 926 723 23 435 671 1195 901
Cuts at: 5891 6153 820
Size: 262 1553
Fragments arranged by size:
1553 1195 926 901 723 671 435 262
197 23
SnaBI TAC'GTA
Cuts at: 493 4183 4677 493
Size: 3690 494 2702
Fragments arranged by size:
3690 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6886
SphI G_CATG'C
Cuts at: 1132 4244 1132
Size: 3112 3774
SspI AAT'ATT
Cuts at: 5 52 4810 5
Size: 47 4758 2081
Fragments arranged by size:
4758 2081 47
StuI AGG'CCT
Cuts at: 1517 2561 1517
Size: 1044 5842
StyI C'CwwG_G
Cuts at: 513 1065 2760 2947 3706 4325 513
Size: 552 1695 187 759 619 3074
Fragments arranged by size:
3074 1695 759 619 552 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2218 2353 2465 3571 4913 4930 5083
Size: 1255 638 135 112 1106 1342 17 153
Cuts at: 5083 5268 5713 325
Size: 185 445 1498
Fragments arranged by size:
1498 1342 1255 1106 638 445 185 153
135 112 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2457 3201 3549
Size: 274 80 33 51 1443 480 744 348
Cuts at: 3549 5132 6844 96
Size: 1583 1712 138
Fragments arranged by size:
1712 1583 1443 744 480 348 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3021 3053 6846 6878 3021
Size: 32 3793 32 3029
Fragments arranged by size:
3793 3029 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6886
XbaI T'CTAG_A
Cuts at: 2732 3598 4222 2732
Size: 866 624 5396
Fragments arranged by size:
5396 866 624
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2767 3143 3364 2767
Size: 376 221 6289
Fragments arranged by size:
6289 376 221
XhoI C'TCGA_G
Cuts at: 1943 2689 1943
Size: 746 6140
XmaI C'CCGG_G
Cuts at: 1870 4211 1870
Size: 2341 4545
XmnI GAAnn'nnTTC
Cuts at: 3315 5015 3315
Size: 1700 5186
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4694 276
Size: 53 83 186 936 3160 2468
Fragments arranged by size:
3160 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI BstZ17I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PsrI RsrII SacII
SapI SexAI SfiI SfoI SrfI SwaI