Restriction map for
pFN21ASDA1263
(Circular) (Six-base) MAPSORT of: pFN21ASDA1263.seq Check: 3609 from: 1 to: 7267
pFN21ASDA1263 7267 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2987 2987
Size: 7267
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5077 278
Size: 53 83 186 936 3541 2468
Fragments arranged by size:
3541 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4610 1937
Size: 2673 4594
Acc65I G'GTAC_C
Cuts at: 3704 4588 3704
Size: 884 6383
AclI AA'CG_TT
Cuts at: 1563 5394 5767 1563
Size: 3831 373 3063
Fragments arranged by size:
3831 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4287 4539 5330 6714 6982 1632
Size: 2655 252 791 1384 268 1917
Fragments arranged by size:
2655 1917 1384 791 268 252
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7070
AflIII A'CryG_T
Cuts at: 1203 3375 6166 7143 1203
Size: 2172 2791 977 1327
Fragments arranged by size:
2791 2172 1327 977
AgeI A'CCGG_T
Cuts at: 6098 6098
Size: 7267
AhdI GACnn_n'nnGTC
Cuts at: 5996 5996
Size: 7267
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7267
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2385 1184
Size: 1201 6066
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3495 3527 3495
Size: 32 7235
AlwNI CAG_nnn'CTG
Cuts at: 2801 3644 5010 6582 2801
Size: 843 1366 1572 3486
Fragments arranged by size:
3486 1572 1366 843
ApaLI G'TGCA_C
Cuts at: 2360 5324 6480 2360
Size: 2964 1156 3147
Fragments arranged by size:
3147 2964 1156
ApoI r'AATT_y
Cuts at: 1493 2336 2729 3431 4576 4802 4954 7063 1493
Size: 843 393 702 1145 226 152 2109 1697
Fragments arranged by size:
2109 1697 1145 843 702 393 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2610 4146 5821 160
Size: 872 1578 1536 1675 1606
Fragments arranged by size:
1675 1606 1578 1536 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7267
AvaI C'yCGr_G
Cuts at: 1870 1943 4592 1870
Size: 73 2649 4545
Fragments arranged by size:
4545 2649 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3241 3274 3241
Size: 33 7234
BamHI G'GATC_C
Cuts at: 4597 4964 4597
Size: 367 6900
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3102 3704 4588 6043 618
Size: 325 217 627 1315 602 884 1455 1842
Fragments arranged by size:
1842 1455 1315 884 627 602 325 217
BanII G_rGCy'C
Cuts at: 1391 4332 4586 1391
Size: 2941 254 4072
Fragments arranged by size:
4072 2941 254
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2245 2418 2910 3294 3318 3722 928
Size: 971 346 173 492 384 24 404 4473
Fragments arranged by size:
4473 971 492 404 384 346 173 24
BbvCI CC'TCA_GC
Cuts at: 3129 3498 3129
Size: 369 6898
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5458 5492 1903
Size: 34 3521 34 3678
Fragments arranged by size:
3678 3521 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3066 5159 6369 3066
Size: 2093 1210 3964
Fragments arranged by size:
3964 2093 1210
BclI T'GATC_A
Cuts at: 1548 3679 1548
Size: 2131 5136
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5878 136
Size: 107 122 71 631 605 4206 1525
Fragments arranged by size:
4206 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7262 7262
Size: 7267
BlpI GC'TnA_GC
Cuts at: 3695 4684 3695
Size: 989 6278
Bme1580I G_kGCm'C
Cuts at: 1790 2364 4106 5328 6484 1790
Size: 574 1742 1222 1156 2573
Fragments arranged by size:
2573 1742 1222 1156 574
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7267
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3644 4998 5956
Size: 416 53 83 399 24 2227 1354 958
Cuts at: 5956 442
Size: 1753
Fragments arranged by size:
2227 1753 1354 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7267
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2090 3024 3264 4104 5927 1239
Size: 851 934 240 840 1823 2579
Fragments arranged by size:
2579 1823 934 851 840 240
Bpu10I CC'TnA_GC
Cuts at: 3129 3498 3129
Size: 369 6898
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4349 4361 5392 6257 6555 4349
Size: 12 1031 865 298 5061
Fragments arranged by size:
5061 1031 865 298 12
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2842 5930 882
Size: 617 1343 3088 2219
Fragments arranged by size:
3088 2219 1343 617
BsaAI yAC'GTr
Cuts at: 493 1204 4564 5058 493
Size: 711 3360 494 2702
Fragments arranged by size:
3360 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7261 7261
Size: 7267
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5074 5456 6091 275
Size: 53 83 186 936 3541 382 635 1451
Fragments arranged by size:
3541 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4028 4401 5700 6098 6372 6519 6930 4028
Size: 373 1299 398 274 147 411 4365
Fragments arranged by size:
4365 1299 411 398 373 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3495 3525 4090 4120 1178
Size: 30 165 30 2092 30 565 30 4325
Fragments arranged by size:
4325 2092 565 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4206 1186
Size: 3020 4247
BseYI C'CCAG_C
Cuts at: 3699 6470 6966 3699
Size: 2771 496 4000
Fragments arranged by size:
4000 2771 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3626 3876 4214 1163
Size: 573 1890 250 338 4216
Fragments arranged by size:
4216 1890 573 338 250
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4717 5478 6506 1540
Size: 275 186 2716 761 1028 2301
Fragments arranged by size:
2716 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2364 3638 4320 4332 5328 5413 6484 2364
Size: 1274 682 12 996 85 1071 3147
Fragments arranged by size:
3147 1274 1071 996 682 85 12
BsmI GAATG_Cn'
Cuts at: 2741 4778 4871 2741
Size: 2037 93 5137
Fragments arranged by size:
5137 2037 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2364 3638 4106 4320 4332 4586 5328
Size: 399 574 1274 468 214 12 254 742
Cuts at: 5328 5413 6484 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1274 1071 742 574 468 399 254
214 85 12
BspEI T'CCGG_A
Cuts at: 6930 6930
Size: 7267
BspHI T'CATG_A
Cuts at: 5156 6886 5156
Size: 1730 5537
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2987 4622 844
Size: 2143 1635 3489
Fragments arranged by size:
3489 2143 1635
BsrBI CCG'CTC
Cuts at: 1394 1823 5154 1394
Size: 429 3331 3507
Fragments arranged by size:
3507 3331 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3229 5762 5936 65
Size: 1183 1981 2533 174 1396
Fragments arranged by size:
2533 1981 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5911 6098 1700
Size: 110 4101 187 2869
Fragments arranged by size:
4101 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7267
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7267
BssSI C'ACGA_G
Cuts at: 5327 6339 5327
Size: 1012 6255
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2279 794
Size: 1485 5782
BstBI TT'CG_AA
Cuts at: 2007 6071 2007
Size: 4064 3203
BstEII G'GTnAC_C
Cuts at: 1184 2950 1184
Size: 1766 5501
BstXI CCAn_nnnn'nTGG
Cuts at: 4427 4427
Size: 7267
BstYI r'GATC_y
Cuts at: 1972 2207 2324 2653 4597 4964 5349 5366 6818
Size: 235 117 329 1944 367 385 17 1452
Cuts at: 6818 6904 6916 7262 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1944 1452 385 367 346 329 235
117 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6715
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6528
Fragments arranged by size:
6528 683 56
BtsI GCAGTG_nn'
Cuts at: 2790 3211 3636 4783 5577 5597 2790
Size: 421 425 1147 794 20 4460
Fragments arranged by size:
4460 1147 794 425 421 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7267
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7232
DraI TTT'AAA
Cuts at: 2425 2572 4571 2425
Size: 147 1999 5121
Fragments arranged by size:
5121 1999 147
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6274 809
Size: 507 4958 1802
Fragments arranged by size:
4958 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3747 4126 4714
Size: 54 1425 50 275 18 1917 379 588
Cuts at: 4714 5603 7232 8
Size: 889 1629 43
Fragments arranged by size:
1917 1629 1425 889 588 379 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4714 1537
Size: 275 2902 4090
Fragments arranged by size:
4090 2902 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3301 4339 4478 5197 1353
Size: 1948 1038 139 719 3423
Fragments arranged by size:
3423 1948 1038 719 139
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5854 6228 6374 387
Size: 1064 138 4265 374 146 1280
Fragments arranged by size:
4265 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4330 4330
Size: 7267
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 3024 3264 4104 4287 4539 5330
Size: 393 458 934 240 840 183 252 791
Cuts at: 5330 5927 6714 6982 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 934 840 791 787 597 458 393
268 252 240 183
EcoO109I rG'GnC_Cy
Cuts at: 2931 2931
Size: 7267
EcoRI G'AATT_C
Cuts at: 2336 4576 4954 2336
Size: 2240 378 4649
Fragments arranged by size:
4649 2240 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2674 2674
Size: 7267
FspI TGC'GCA
Cuts at: 5773 5773
Size: 7267
HaeII r_GCGC'y
Cuts at: 1678 6414 1678
Size: 4736 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2522 2554 2915 2947 3593
Size: 32 135 32 950 32 361 32 646
Cuts at: 3593 3625 3704 3736 4090 4122 4345 4377 5908
Size: 32 79 32 354 32 223 32 1531
Cuts at: 5908 5940 5982 6014 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1531 950 646 361 354 223 135
79 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4611 4857 669
Size: 988 281 2673 246 3079
Fragments arranged by size:
3079 2673 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3170 748
Size: 2422 4845
HpaI GTT'AAC
Cuts at: 4857 4857
Size: 7267
KpnI G_GTAC'C
Cuts at: 3708 4592 3708
Size: 884 6383
MfeI C'AATT_G
Cuts at: 4866 4866
Size: 7267
MluI A'CGCG_T
Cuts at: 7143 7143
Size: 7267
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2055 4531 6381 6565 1305
Size: 750 2476 1850 184 2007
Fragments arranged by size:
2476 2007 1850 750 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3749 4128 10
Size: 54 1425 343 1917 379 3149
Fragments arranged by size:
3149 1917 1425 379 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3050 3854 3882 5225 5584 5743 518
Size: 648 1884 804 28 1343 359 159 2042
Fragments arranged by size:
2042 1884 1343 804 648 359 159 28
MspA1I CmG'CkG
Cuts at: 1606 3536 4683 5360 6508 6753 1606
Size: 1930 1147 677 1148 245 2120
Fragments arranged by size:
2120 1930 1148 1147 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6715
NdeI CA'TA_TG
Cuts at: 387 3855 4111 387
Size: 3468 256 3543
Fragments arranged by size:
3543 3468 256
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7267
NotI GC'GGCC_GC
Cuts at: 4714 4714
Size: 7267
NspI r_CATG'y
Cuts at: 1132 1229 3379 4625 6170 1132
Size: 97 2150 1246 1545 2229
Fragments arranged by size:
2229 2150 1545 1246 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7267
PciI A'CATG_T
Cuts at: 3375 6166 3375
Size: 2791 4476
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2862 3790 1108
Size: 1754 928 4585
Fragments arranged by size:
4585 1754 928
PfoI T'CCnGG_A
Cuts at: 4419 4419
Size: 7267
PmeI GTTT'AAAC
Cuts at: 4571 4571
Size: 7267
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3495 3527 5336 5368 6849 6881 3495
Size: 32 1809 32 1481 32 3881
Fragments arranged by size:
3881 1809 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2931 2931
Size: 7267
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7267
PsiI TTA'TAA
Cuts at: 4837 4837
Size: 7267
PstI C_TGCA'G
Cuts at: 830 2797 4619 830
Size: 1967 1822 3478
Fragments arranged by size:
3478 1967 1822
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7267
PvuII CAG'CTG
Cuts at: 3536 3536
Size: 7267
SacI G_AGCT'C
Cuts at: 4332 4332
Size: 7267
SalI G'TCGA_C
Cuts at: 1936 4609 1936
Size: 2673 4594
SbfI CC_TGCA'GG
Cuts at: 4619 4619
Size: 7267
ScaI AGT'ACT
Cuts at: 2225 5515 7227 2225
Size: 3290 1712 2265
Fragments arranged by size:
3290 2265 1712
SfcI C'TryA_G
Cuts at: 826 1045 2793 3731 4615 5750 6431 6622 826
Size: 219 1748 938 884 1135 681 191 1471
Fragments arranged by size:
1748 1471 1135 938 884 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7267
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7267
SmaI CCC'GGG
Cuts at: 1872 4594 1872
Size: 2722 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4364 4376 5371 6272 6534 820
Size: 197 926 2421 12 995 901 262 1553
Fragments arranged by size:
2421 1553 995 926 901 262 197 12
SnaBI TAC'GTA
Cuts at: 493 4564 5058 493
Size: 4071 494 2702
Fragments arranged by size:
4071 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7267
SphI G_CATG'C
Cuts at: 1132 4625 1132
Size: 3493 3774
SspI AAT'ATT
Cuts at: 5 52 2576 2878 5191 5
Size: 47 2524 302 2313 2081
Fragments arranged by size:
2524 2313 2081 302 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7267
StyI C'CwwG_G
Cuts at: 513 1065 3466 3708 4394 4461 4706 513
Size: 552 2401 242 686 67 245 3074
Fragments arranged by size:
3074 2401 686 552 245 242 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3679 3797 3866 5294 5311 5464 5649
Size: 1255 2099 118 69 1428 17 153 185
Cuts at: 5649 6094 325
Size: 445 1498
Fragments arranged by size:
2099 1498 1428 1255 445 185 153 118
69 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2223 2666 4176
Size: 274 80 33 51 1443 246 443 1510
Cuts at: 4176 5513 7225 96
Size: 1337 1712 138
Fragments arranged by size:
1712 1510 1443 1337 443 274 246 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3393 3425 3941 3973 7227 7259 3393
Size: 32 516 32 3254 32 3401
Fragments arranged by size:
3401 3254 516 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3587 3644 4073 1316
Size: 2271 57 429 4510
Fragments arranged by size:
4510 2271 429 57
XbaI T'CTAG_A
Cuts at: 4603 4603
Size: 7267
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3860 3860
Size: 7267
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7267
XmaI C'CCGG_G
Cuts at: 1870 4592 1870
Size: 2722 4545
XmnI GAAnn'nnTTC
Cuts at: 3377 5396 3377
Size: 2019 5248
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5075 276
Size: 53 83 186 936 3541 2468
Fragments arranged by size:
3541 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI