Restriction map for
pFN21ASDA1259
(Circular) (Six-base) MAPSORT of: pFN21ASDA1259.seq Check: 8759 from: 1 to: 9397
pFN21ASDA1259 9397 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3754 3754
Size: 9397
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7207 278
Size: 53 83 186 936 5671 2468
Fragments arranged by size:
5671 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3752 6740 1937
Size: 1815 2988 4594
Fragments arranged by size:
4594 2988 1815
Acc65I G'GTAC_C
Cuts at: 6718 6718
Size: 9397
AclI AA'CG_TT
Cuts at: 1563 7524 7897 1563
Size: 5961 373 3063
Fragments arranged by size:
5961 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2072 2399 2754 2898 3398 4724 5156 7460
Size: 440 327 355 144 500 1326 432 2304
Cuts at: 7460 8844 9112 1632
Size: 1384 268 1917
Fragments arranged by size:
2304 1917 1384 1326 500 440 432 355
327 268 144
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9200
AflIII A'CryG_T
Cuts at: 1203 6465 8296 9273 1203
Size: 5262 1831 977 1327
Fragments arranged by size:
5262 1831 1327 977
AgeI A'CCGG_T
Cuts at: 8228 8228
Size: 9397
AhdI GACnn_n'nnGTC
Cuts at: 8126 8126
Size: 9397
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9397
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2859 6134 6176 6512 1184
Size: 1675 3275 42 336 4069
Fragments arranged by size:
4069 3275 1675 336 42
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4352 4384 6576 6608 4352
Size: 32 2192 32 7141
Fragments arranged by size:
7141 2192 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3376 4339 4950 7140 8712 3376
Size: 963 611 2190 1572 4061
Fragments arranged by size:
4061 2190 1572 963 611
ApaI G_GGCC'C
Cuts at: 2107 5185 5653 2107
Size: 3078 468 5851
Fragments arranged by size:
5851 3078 468
ApaLI G'TGCA_C
Cuts at: 7454 8610 7454
Size: 1156 8241
ApoI r'AATT_y
Cuts at: 1493 2254 2356 2654 3797 4145 4365 6261 6706
Size: 761 102 298 1143 348 220 1896 445
Cuts at: 6706 6932 7084 9193 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1896 1697 1143 761 445 348 298
226 220 152 102
AseI AT'TA_AT
Cuts at: 160 1032 3633 4668 7951 160
Size: 872 2601 1035 3283 1606
Fragments arranged by size:
3283 2601 1606 1035 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9397
AvaI C'yCGr_G
Cuts at: 1870 1943 6204 6722 1870
Size: 73 4261 518 4545
Fragments arranged by size:
4545 4261 518 73
BamHI G'GATC_C
Cuts at: 2221 6727 7094 2221
Size: 4506 367 4524
Fragments arranged by size:
4524 4506 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2039 2628 3768 5705 6668
Size: 325 217 627 252 589 1140 1937 963
Cuts at: 6668 6718 8173 618
Size: 50 1455 1842
Fragments arranged by size:
1937 1842 1455 1140 963 627 589 325
252 217 50
BanII G_rGCy'C
Cuts at: 1391 2107 4102 4403 5185 5653 6716 1391
Size: 716 1995 301 782 468 1063 4072
Fragments arranged by size:
4072 1995 1063 782 716 468 301
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2457 3285 3872 4383 4570 5813 928
Size: 971 558 828 587 511 187 1243 4512
Fragments arranged by size:
4512 1243 971 828 587 558 511 187
BbvCI CC'TCA_GC
Cuts at: 4253 6190 4253
Size: 1937 7460
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7588 7622 1903
Size: 34 5651 34 3678
Fragments arranged by size:
5651 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3260 7289 8499 3260
Size: 4029 1210 4158
Fragments arranged by size:
4158 4029 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9397
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2993 3467 3692
Size: 107 122 71 631 605 1321 474 225
Cuts at: 3692 3701 8008 136
Size: 9 4307 1525
Fragments arranged by size:
4307 1525 1321 631 605 474 225 122
107 71 9
BglII A'GATC_T
Cuts at: 5478 6099 9392 5478
Size: 621 3293 5483
Fragments arranged by size:
5483 3293 621
BlpI GC'TnA_GC
Cuts at: 2867 6814 2867
Size: 3947 5450
Bme1580I G_kGCm'C
Cuts at: 1790 2107 3031 5185 5653 7458 8614 1790
Size: 317 924 2154 468 1805 1156 2573
Fragments arranged by size:
2573 2154 1805 1156 924 468 317
BmgBI CAC'GTC
Cuts at: 1318 6293 1318
Size: 4975 4422
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2206 3498 5573
Size: 416 53 83 399 24 789 1292 2075
Cuts at: 5573 7128 8086 442
Size: 1555 958 1753
Fragments arranged by size:
2075 1753 1555 1292 958 789 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9397
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2298 3877 4698 5262 5441 5577 6702 8057
Size: 1059 1579 821 564 179 136 1125 1355
Cuts at: 8057 1239
Size: 2579
Fragments arranged by size:
2579 1579 1355 1125 1059 821 564 179
136
Bpu10I CC'TnA_GC
Cuts at: 3984 4253 5659 6190 3984
Size: 269 1406 531 7191
Fragments arranged by size:
7191 1406 531 269
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3977 5345 5352 7522 8387 8685 3977
Size: 1368 7 2170 865 298 4689
Fragments arranged by size:
4689 2170 1368 865 298 7
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4512 4650 8060 882
Size: 617 3013 138 3410 2219
Fragments arranged by size:
3410 3013 2219 617 138
BsaAI yAC'GTr
Cuts at: 493 1204 6694 7188 493
Size: 711 5490 494 2702
Fragments arranged by size:
5490 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 4209 6104 9391 4209
Size: 1895 3287 4215
Fragments arranged by size:
4215 3287 1895
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7204 7586 8221 275
Size: 53 83 186 936 5671 382 635 1451
Fragments arranged by size:
5671 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2109 6651 7830 8228 8502 8649 9060 2109
Size: 4542 1179 398 274 147 411 2446
Fragments arranged by size:
4542 2446 1179 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3306 3309 3336 3339 4492
Size: 30 165 30 1903 3 27 3 1153
Cuts at: 4492 4522 6113 6143 6170 6200 6202 6232 1178
Size: 30 1591 30 27 30 2 30 4343
Fragments arranged by size:
4343 1903 1591 1153 165 30 30 30
30 30 30 27 27 3 3 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3330 4270 4999 6178 1186
Size: 2144 940 729 1179 4405
Fragments arranged by size:
4405 2144 1179 940 729
BseYI C'CCAG_C
Cuts at: 6181 8600 9096 6181
Size: 2419 496 6482
Fragments arranged by size:
6482 2419 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2044 2604 3947 5133 6104 1163
Size: 573 308 560 1343 1186 971 4456
Fragments arranged by size:
4456 1343 1186 971 573 560 308
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6847 7608 8636 1540
Size: 275 186 4846 761 1028 2301
Fragments arranged by size:
4846 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3093 7458 7543 8614 3093
Size: 4365 85 1071 3876
Fragments arranged by size:
4365 3876 1071 85
BsmI GAATG_Cn'
Cuts at: 2406 2848 3388 3639 5721 6908 7001 2406
Size: 442 540 251 2082 1187 93 4802
Fragments arranged by size:
4802 2082 1187 540 442 251 93
BsmBI CGTCTCn'nnnn_
Cuts at: 6247 6247
Size: 9397
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2107 3031 3093 4102 4403 5185 5653
Size: 399 317 924 62 1009 301 782 468
Cuts at: 5653 6716 7458 7543 8614 1391
Size: 1063 742 85 1071 2174
Fragments arranged by size:
2174 1071 1063 1009 924 782 742 468
399 317 301 85 62
BspEI T'CCGG_A
Cuts at: 6651 9060 6651
Size: 2409 6988
BspHI T'CATG_A
Cuts at: 3216 3241 3829 4437 7286 9016 3216
Size: 25 588 608 2849 1730 3597
Fragments arranged by size:
3597 2849 1730 608 588 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3754 5665 6752 844
Size: 2910 1911 1087 3489
Fragments arranged by size:
3489 2910 1911 1087
BsrBI CCG'CTC
Cuts at: 1394 1823 7284 1394
Size: 429 5461 3507
Fragments arranged by size:
5461 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2258 3681 5107 6405 7892 8066 65
Size: 1183 1010 1423 1426 1298 1487 174 1396
Fragments arranged by size:
1487 1426 1423 1396 1298 1183 1010 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5688 8041 8228 1700
Size: 110 3878 2353 187 2869
Fragments arranged by size:
3878 2869 2353 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9397
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9397
BssSI C'ACGA_G
Cuts at: 3024 4590 5067 5550 7457 8469 3024
Size: 1566 477 483 1907 1012 3952
Fragments arranged by size:
3952 1907 1566 1012 483 477
BstAPI GCAn_nnn'nTGC
Cuts at: 794 6389 794
Size: 5595 3802
BstBI TT'CG_AA
Cuts at: 2007 8201 2007
Size: 6194 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9397
BstXI CCAn_nnnn'nTGG
Cuts at: 5812 6139 5812
Size: 327 9070
BstYI r'GATC_y
Cuts at: 1972 2221 4020 5478 6099 6727 7094 7479 7496
Size: 249 1799 1458 621 628 367 385 17
Cuts at: 7496 8948 9034 9046 9392 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1799 1458 1452 628 621 385 367
346 249 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3266 4853 3266
Size: 1587 7810
BtgI C'CryG_G
Cuts at: 513 1065 2022 4083 5640 6378 513
Size: 552 957 2061 1557 738 3532
Fragments arranged by size:
3532 2061 1557 957 738 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8658
Fragments arranged by size:
8658 683 56
BtsI GCAGTG_nn'
Cuts at: 4965 5085 5171 5577 6118 6160 6394 6913 7707
Size: 120 86 406 541 42 234 519 794
Cuts at: 7707 7727 4965
Size: 20 6635
Fragments arranged by size:
6635 794 541 519 406 234 120 86
42 20
ClaI AT'CG_AT
Cuts at: 1554 3303 1554
Size: 1749 7648
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9362
DraI TTT'AAA
Cuts at: 3364 6701 3364
Size: 3337 6060
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4982 6164 6647 8404 809
Size: 507 3666 1182 483 1757 1802
Fragments arranged by size:
3666 1802 1757 1182 507 483
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3609 4752 5058
Size: 54 1425 50 275 18 1779 1143 306
Cuts at: 5058 6844 7733 9362 8
Size: 1786 889 1629 43
Fragments arranged by size:
1786 1779 1629 1425 1143 889 306 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6844 1537
Size: 275 5032 4090
Fragments arranged by size:
5032 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2707 5738 5832 5865 6559 7327 1353
Size: 1354 3031 94 33 694 768 3423
Fragments arranged by size:
3423 3031 1354 768 694 94 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6472 7984 8358 8504 387
Size: 1064 138 4883 1512 374 146 1280
Fragments arranged by size:
4883 1512 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2298 2399 2754 2898 3398 3877
Size: 393 440 226 101 355 144 500 479
Cuts at: 3877 4698 4724 5156 5262 5441 5577 6702 7460
Size: 821 26 432 106 179 136 1125 758
Cuts at: 7460 8057 8844 9112 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1125 821 787 758 597 500 479
440 432 393 355 268 226 179 144
136 106 101 26
EcoNI CCTnn'n_nnAGG
Cuts at: 3041 4792 5420 5455 3041
Size: 1751 628 35 6983
Fragments arranged by size:
6983 1751 628 35
EcoO109I rG'GnC_Cy
Cuts at: 2103 2104 3373 3725 4065 5181 5182 5380 5649
Size: 1 1269 352 340 1116 1 198 269
Cuts at: 5649 2103
Size: 5851
Fragments arranged by size:
5851 1269 1116 352 340 269 198 1
1
EcoRI G'AATT_C
Cuts at: 6706 7084 6706
Size: 378 9019
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3034 4042 6570 3034
Size: 1008 2528 5861
Fragments arranged by size:
5861 2528 1008
FspI TGC'GCA
Cuts at: 7903 7903
Size: 9397
HaeII r_GCGC'y
Cuts at: 1678 3992 4826 6552 8544 1678
Size: 2314 834 1726 1992 2531
Fragments arranged by size:
2531 2314 1992 1726 834
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5027 5059 5456 5488 5553
Size: 32 135 32 3455 32 397 32 65
Cuts at: 5553 5585 6170 6202 8038 8070 8112 8144 1373
Size: 32 585 32 1836 32 42 32 2626
Fragments arranged by size:
3455 2626 1836 585 397 135 65 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3328 6741 6987 669
Size: 988 281 1390 3413 246 3079
Fragments arranged by size:
3413 3079 1390 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2910 2948 3114 748
Size: 2162 38 166 7031
Fragments arranged by size:
7031 2162 166 38
HpaI GTT'AAC
Cuts at: 3328 6987 3328
Size: 3659 5738
KpnI G_GTAC'C
Cuts at: 6722 6722
Size: 9397
MfeI C'AATT_G
Cuts at: 3763 6996 3763
Size: 3233 6164
MluI A'CGCG_T
Cuts at: 9273 9273
Size: 9397
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2094 3114 3995 4022 4663 5823 6054 8511
Size: 789 1020 881 27 641 1160 231 2457
Cuts at: 8511 8695 1305
Size: 184 2007
Fragments arranged by size:
2457 2007 1160 1020 881 789 641 231
184 27
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3611 4754 5060 10
Size: 54 1425 343 1779 1143 306 4347
Fragments arranged by size:
4347 1779 1425 1143 343 306 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5531 6065 7355 7714 7873 518
Size: 648 4365 534 1290 359 159 2042
Fragments arranged by size:
4365 2042 1290 648 534 359 159
MspA1I CmG'CkG
Cuts at: 1606 2839 3127 3458 3919 3949 4914 4993 5240
Size: 1233 288 331 461 30 965 79 247
Cuts at: 5240 5758 6284 6428 6440 6813 7490 8638 8883
Size: 518 526 144 12 373 677 1148 245
Cuts at: 8883 1606
Size: 2120
Fragments arranged by size:
2120 1233 1148 965 677 526 518 461
373 331 288 247 245 144 79 30
12
NcoI C'CATG_G
Cuts at: 513 1065 2022 4083 5640 513
Size: 552 957 2061 1557 4270
Fragments arranged by size:
4270 2061 1557 957 552
NdeI CA'TA_TG
Cuts at: 387 2602 387
Size: 2215 7182
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9397
NotI GC'GGCC_GC
Cuts at: 6844 6844
Size: 9397
NspI r_CATG'y
Cuts at: 1132 1229 2503 6163 6755 8300 1132
Size: 97 1274 3660 592 1545 2229
Fragments arranged by size:
3660 2229 1545 1274 592 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9397
PciI A'CATG_T
Cuts at: 8296 8296
Size: 9397
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 9397
PfoI T'CCnGG_A
Cuts at: 3252 3252
Size: 9397
PmeI GTTT'AAAC
Cuts at: 6701 6701
Size: 9397
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2460 2492 7466 7498 8979 9011 2460
Size: 32 4974 32 1481 32 2846
Fragments arranged by size:
4974 2846 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3725 4065 5380 3725
Size: 340 1315 7742
Fragments arranged by size:
7742 1315 340
PshAI GACnn'nnGTC
Cuts at: 1613 2134 2768 1613
Size: 521 634 8242
Fragments arranged by size:
8242 634 521
PsiI TTA'TAA
Cuts at: 6967 6967
Size: 9397
PspOMI G'GGCC_C
Cuts at: 2103 5181 5649 2103
Size: 3078 468 5851
Fragments arranged by size:
5851 3078 468
PstI C_TGCA'G
Cuts at: 830 5200 5587 6289 6427 6439 6501 6749 830
Size: 4370 387 702 138 12 62 248 3478
Fragments arranged by size:
4370 3478 702 387 248 138 62 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9397
PvuII CAG'CTG
Cuts at: 2839 3458 3919 4914 5240 6284 6440 2839
Size: 619 461 995 326 1044 156 5796
Fragments arranged by size:
5796 1044 995 619 461 326 156
SalI G'TCGA_C
Cuts at: 1936 6739 1936
Size: 4803 4594
SanDI GG'GwC_CC
Cuts at: 4065 4065
Size: 9397
SbfI CC_TGCA'GG
Cuts at: 6749 6749
Size: 9397
ScaI AGT'ACT
Cuts at: 2093 2576 7645 9357 2093
Size: 483 5069 1712 2133
Fragments arranged by size:
5069 2133 1712 483
SexAI A'CCwGG_T
Cuts at: 4977 5994 4977
Size: 1017 8380
SfcI C'TryA_G
Cuts at: 826 1045 2327 2578 4792 5196 5583 6285 6423
Size: 219 1282 251 2214 404 387 702 138
Cuts at: 6423 6435 6497 6745 7880 8561 8752 826
Size: 12 62 248 1135 681 191 1471
Fragments arranged by size:
2214 1471 1282 1135 702 681 404 387
251 248 219 191 138 62 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9397
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9397
SmaI CCC'GGG
Cuts at: 1872 6724 1872
Size: 4852 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3992 5360 5367 6204 7501 8402
Size: 197 926 2049 1368 7 837 1297 901
Cuts at: 8402 8664 820
Size: 262 1553
Fragments arranged by size:
2049 1553 1368 1297 926 901 837 262
197 7
SnaBI TAC'GTA
Cuts at: 493 6694 7188 493
Size: 6201 494 2702
Fragments arranged by size:
6201 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9397
SphI G_CATG'C
Cuts at: 1132 6755 1132
Size: 5623 3774
SspI AAT'ATT
Cuts at: 5 52 2158 7321 5
Size: 47 2106 5163 2081
Fragments arranged by size:
5163 2106 2081 47
StuI AGG'CCT
Cuts at: 1517 2589 1517
Size: 1072 8325
StyI C'CwwG_G
Cuts at: 513 1065 2022 3042 3661 4083 4164 5640 5736
Size: 552 957 1020 619 422 81 1476 96
Cuts at: 5736 6836 513
Size: 1100 3074
Fragments arranged by size:
3074 1476 1100 1020 957 619 552 422
96 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2027 5121 7424 7441 7594 7779 8224
Size: 1255 447 3094 2303 17 153 185 445
Cuts at: 8224 325
Size: 1498
Fragments arranged by size:
3094 2303 1498 1255 447 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2091 2574 3057
Size: 274 80 33 51 1443 114 483 483
Cuts at: 3057 3673 7643 9355 96
Size: 616 3970 1712 138
Fragments arranged by size:
3970 1712 1443 616 483 483 274 138
114 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5211 5243 6113 6145 9357 9389 5211
Size: 32 870 32 3212 32 5219
Fragments arranged by size:
5219 3212 870 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3421 6294 1316
Size: 2105 2873 4419
Fragments arranged by size:
4419 2873 2105
XbaI T'CTAG_A
Cuts at: 5827 6733 5827
Size: 906 8491
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3916 3916
Size: 9397
XhoI C'TCGA_G
Cuts at: 1943 6204 1943
Size: 4261 5136
XmaI C'CCGG_G
Cuts at: 1870 6722 1870
Size: 4852 4545
XmnI GAAnn'nnTTC
Cuts at: 2490 5721 7526 2490
Size: 3231 1805 4361
Fragments arranged by size:
4361 3231 1805
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7205 276
Size: 53 83 186 936 5671 2468
Fragments arranged by size:
5671 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SacI SacII SapI SfiI SfoI SrfI SwaI