Restriction map for
pFN21ASDA1257
(Circular) (Six-base) MAPSORT of: pFN21ASDA1257.seq Check: 9828 from: 1 to: 5956
pFN21ASDA1257 5956 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3766 278
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3299 1937
Size: 1362 4594
Acc65I G'GTAC_C
Cuts at: 3191 3277 3191
Size: 86 5870
AclI AA'CG_TT
Cuts at: 1563 4083 4456 1563
Size: 2520 373 3063
Fragments arranged by size:
3063 2520 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2153 4019 5403 5671 1632
Size: 521 1866 1384 268 1917
Fragments arranged by size:
1917 1866 1384 521 268
AfeI AGC'GCT
Cuts at: 3151 3151
Size: 5956
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5759
AflIII A'CryG_T
Cuts at: 1203 4855 5832 1203
Size: 3652 977 1327
Fragments arranged by size:
3652 1327 977
AgeI A'CCGG_T
Cuts at: 4787 4787
Size: 5956
AhdI GACnn_n'nnGTC
Cuts at: 2614 4685 2614
Size: 2071 3885
AleI CACnn'nnGTG
Cuts at: 1166 2496 1166
Size: 1330 4626
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5956
AlwNI CAG_nnn'CTG
Cuts at: 3223 3699 5271 3223
Size: 476 1572 3908
Fragments arranged by size:
3908 1572 476
ApaI G_GGCC'C
Cuts at: 2152 2152
Size: 5956
ApaLI G'TGCA_C
Cuts at: 4013 5169 4013
Size: 1156 4800
ApoI r'AATT_y
Cuts at: 1493 2249 2569 3265 3491 3643 5752 1493
Size: 756 320 696 226 152 2109 1697
Fragments arranged by size:
2109 1697 756 696 320 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2984 4510 160
Size: 872 1952 1526 1606
Fragments arranged by size:
1952 1606 1526 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
AvaI C'yCGr_G
Cuts at: 1870 1943 3281 1870
Size: 73 1338 4545
Fragments arranged by size:
4545 1338 73
BamHI G'GATC_C
Cuts at: 3286 3653 3286
Size: 367 5589
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3191 3277 4732 618
Size: 325 217 627 1404 86 1455 1842
Fragments arranged by size:
1842 1455 1404 627 325 217 86
BanII G_rGCy'C
Cuts at: 1391 2073 2152 2850 3275 1391
Size: 682 79 698 425 4072
Fragments arranged by size:
4072 698 682 425 79
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4985
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2167 2201 4147 4181 1903
Size: 34 230 34 1946 34 3678
Fragments arranged by size:
3678 1946 230 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3848 5058 3848
Size: 1210 4746
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5956
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3244 4567 136
Size: 107 122 71 631 605 1572 1323 1525
Fragments arranged by size:
1572 1525 1323 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5951 5951
Size: 5956
BlpI GC'TnA_GC
Cuts at: 3373 3373
Size: 5956
Bme1580I G_kGCm'C
Cuts at: 1790 2152 2232 4017 5173 1790
Size: 362 80 1785 1156 2573
Fragments arranged by size:
2573 1785 1156 362 80
BmgBI CAC'GTC
Cuts at: 1318 2119 2227 2668 1318
Size: 801 108 441 4606
Fragments arranged by size:
4606 801 441 108
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3687 4645 442
Size: 416 53 83 399 24 2270 958 1753
Fragments arranged by size:
2270 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5956
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2092 2179 2092
Size: 87 5869
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2480 4616 1239
Size: 1241 2136 2579
Fragments arranged by size:
2579 2136 1241
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2608 4081 4946 5244 2608
Size: 1473 865 298 3320
Fragments arranged by size:
3320 1473 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3042 4619 882
Size: 617 1543 1577 2219
Fragments arranged by size:
2219 1577 1543 617
BsaAI yAC'GTr
Cuts at: 493 1204 2236 3253 3747 493
Size: 711 1032 1017 494 2702
Fragments arranged by size:
2702 1032 1017 711 494
BsaBI GATnn'nnATC
Cuts at: 2972 5950 2972
Size: 2978 2978
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3763 4145 4780 275
Size: 53 83 186 936 2230 382 635 1451
Fragments arranged by size:
2230 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4389 4787 5061 5208 5619 4389
Size: 398 274 147 411 4726
Fragments arranged by size:
4726 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5731
Fragments arranged by size:
5731 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2068 3097 1186
Size: 882 1029 4045
Fragments arranged by size:
4045 1029 882
BseYI C'CCAG_C
Cuts at: 3213 5159 5655 3213
Size: 1946 496 3514
Fragments arranged by size:
3514 1946 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2197 1163
Size: 573 461 4922
Fragments arranged by size:
4922 573 461
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3406 4167 5195 1540
Size: 275 186 1405 761 1028 2301
Fragments arranged by size:
2301 1405 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4017 4102 5173 4017
Size: 85 1071 4800
Fragments arranged by size:
4800 1071 85
BsmI GAATG_Cn'
Cuts at: 3467 3560 3467
Size: 93 5863
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2152 2232 2850 3275 4017 4102
Size: 399 283 79 80 618 425 742 85
Cuts at: 4102 5173 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 618 425 399 283 85
80 79
BspEI T'CCGG_A
Cuts at: 5619 5619
Size: 5956
BspHI T'CATG_A
Cuts at: 3845 5575 3845
Size: 1730 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3311 844
Size: 2467 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3181 3843 1394
Size: 429 1358 662 3507
Fragments arranged by size:
3507 1358 662 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4451 4625 65
Size: 1183 3203 174 1396
Fragments arranged by size:
3203 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2651 3188 4600 4787 1700
Size: 110 841 537 1412 187 2869
Fragments arranged by size:
2869 1412 841 537 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5956
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5956
BssSI C'ACGA_G
Cuts at: 2104 4016 5028 2104
Size: 1912 1012 3032
Fragments arranged by size:
3032 1912 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3223 794
Size: 2429 3527
BstBI TT'CG_AA
Cuts at: 2007 4760 2007
Size: 2753 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5956
BstXI CCAn_nnnn'nTGG
Cuts at: 2263 2950 3166 2263
Size: 687 216 5053
Fragments arranged by size:
5053 687 216
BstYI r'GATC_y
Cuts at: 1972 3286 3653 4038 4055 5507 5593 5605 5951
Size: 1314 367 385 17 1452 86 12 346
Cuts at: 5951 1972
Size: 1977
Fragments arranged by size:
1977 1452 1314 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2486 513
Size: 552 1421 3983
Fragments arranged by size:
3983 1421 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5217
Fragments arranged by size:
5217 683 56
BtsI GCAGTG_nn'
Cuts at: 3196 3472 4266 4286 3196
Size: 276 794 20 4866
Fragments arranged by size:
4866 794 276 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5956
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5921
DraI TTT'AAA
Cuts at: 2709 3260 2709
Size: 551 5405
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4963 809
Size: 507 3647 1802
Fragments arranged by size:
3647 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2445 3168 3236
Size: 54 1425 50 275 18 615 723 68
Cuts at: 3236 3245 3403 4292 5921 8
Size: 9 158 889 1629 43
Fragments arranged by size:
1629 1425 889 723 615 275 158 68
54 50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 3403 1537
Size: 275 1591 4090
Fragments arranged by size:
4090 1591 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2043 2757 3105 3886 1353
Size: 690 714 348 781 3423
Fragments arranged by size:
3423 781 714 690 348
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2772 4543 4917 5063 387
Size: 1064 138 1183 1771 374 146 1280
Fragments arranged by size:
1771 1280 1183 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2153 2480 4019 4616 5403 5671 1239
Size: 393 521 327 1539 597 787 268 1524
Fragments arranged by size:
1539 1524 787 597 521 393 327 268
EcoO109I rG'GnC_Cy
Cuts at: 2053 2148 3134 2053
Size: 95 986 4875
Fragments arranged by size:
4875 986 95
EcoRI G'AATT_C
Cuts at: 3265 3643 3265
Size: 378 5578
FspI TGC'GCA
Cuts at: 4462 4462
Size: 5956
HaeII r_GCGC'y
Cuts at: 1678 3153 5103 1678
Size: 1475 1950 2531
Fragments arranged by size:
2531 1950 1475
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2180 2212 2600 2632 2981
Size: 32 135 32 608 32 388 32 349
Cuts at: 2981 3013 4597 4629 4671 4703 1373
Size: 32 1584 32 42 32 2626
Fragments arranged by size:
2626 1584 608 388 349 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2422 3300 3546 669
Size: 988 281 484 878 246 3079
Fragments arranged by size:
3079 988 878 484 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5956
HpaI GTT'AAC
Cuts at: 3546 3546
Size: 5956
KpnI G_GTAC'C
Cuts at: 3195 3281 3195
Size: 86 5870
MfeI C'AATT_G
Cuts at: 3555 3555
Size: 5956
MluI A'CGCG_T
Cuts at: 5832 5832
Size: 5956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2247 5070 5254 1305
Size: 942 2823 184 2007
Fragments arranged by size:
2823 2007 942 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2447 3238 10
Size: 54 1425 343 615 791 2728
Fragments arranged by size:
2728 1425 791 615 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2496 3914 4273 4432 518
Size: 648 1330 1418 359 159 2042
Fragments arranged by size:
2042 1418 1330 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3166 3372 4049 5197 5442 1606
Size: 1560 206 677 1148 245 2120
Fragments arranged by size:
2120 1560 1148 677 245 206
NaeI GCC'GGC
Cuts at: 2653 2653
Size: 5956
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5404
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5956
NgoMIV G'CCGG_C
Cuts at: 2651 2651
Size: 5956
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5956
NotI GC'GGCC_GC
Cuts at: 3403 3403
Size: 5956
NspI r_CATG'y
Cuts at: 1132 1229 2036 3314 4859 1132
Size: 97 807 1278 1545 2229
Fragments arranged by size:
2229 1545 1278 807 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5956
PciI A'CATG_T
Cuts at: 4855 4855
Size: 5956
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5956
PfoI T'CCnGG_A
Cuts at: 2457 2457
Size: 5956
PmeI GTTT'AAAC
Cuts at: 3260 3260
Size: 5956
PmlI CAC'GTG
Cuts at: 2236 2236
Size: 5956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4025 4057 5538 5570 4025
Size: 32 1481 32 4411
Fragments arranged by size:
4411 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2053 2053
Size: 5956
PshAI GACnn'nnGTC
Cuts at: 1613 3034 1613
Size: 1421 4535
PsiI TTA'TAA
Cuts at: 3526 3526
Size: 5956
PspOMI G'GGCC_C
Cuts at: 2148 2148
Size: 5956
PstI C_TGCA'G
Cuts at: 830 3308 830
Size: 2478 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5956
PvuII CAG'CTG
Cuts at: 3166 3166
Size: 5956
SalI G'TCGA_C
Cuts at: 1936 3298 1936
Size: 1362 4594
SbfI CC_TGCA'GG
Cuts at: 3308 3308
Size: 5956
ScaI AGT'ACT
Cuts at: 4204 5916 4204
Size: 1712 4244
SfcI C'TryA_G
Cuts at: 826 1045 3304 4439 5120 5311 826
Size: 219 2259 1135 681 191 1471
Fragments arranged by size:
2259 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3244 3244
Size: 5956
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5956
SmaI CCC'GGG
Cuts at: 1872 3283 1872
Size: 1411 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2587 4060 4961 5223 820
Size: 197 926 644 1473 901 262 1553
Fragments arranged by size:
1553 1473 926 901 644 262 197
SnaBI TAC'GTA
Cuts at: 493 3253 3747 493
Size: 2760 494 2702
Fragments arranged by size:
2760 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5956
SphI G_CATG'C
Cuts at: 1132 3314 1132
Size: 2182 3774
SspI AAT'ATT
Cuts at: 5 52 3880 5
Size: 47 3828 2081
Fragments arranged by size:
3828 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5956
StyI C'CwwG_G
Cuts at: 513 1065 2139 3395 513
Size: 552 1074 1256 3074
Fragments arranged by size:
3074 1256 1074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2500 2829 3983 4000 4153 4338 4783
Size: 1255 920 329 1154 17 153 185 445
Cuts at: 4783 325
Size: 1498
Fragments arranged by size:
1498 1255 1154 920 445 329 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4202 5914 96
Size: 274 80 33 51 1443 2225 1712 138
Fragments arranged by size:
2225 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2253 2285 5916 5948 2253
Size: 32 3631 32 2261
Fragments arranged by size:
3631 2261 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5956
XbaI T'CTAG_A
Cuts at: 3292 3292
Size: 5956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2455 2455
Size: 5956
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5956
XmaI C'CCGG_G
Cuts at: 1870 3281 1870
Size: 1411 4545
XmnI GAAnn'nnTTC
Cuts at: 4085 4085
Size: 5956
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3764 276
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfoI SrfI SwaI