Restriction map for
pFN21ASDA1253
(Circular) (Six-base) MAPSORT of: pFN21ASDA1253.seq Check: 1261 from: 1 to: 6088
pFN21ASDA1253 6088 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2486 2486
Size: 6088
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3898 278
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2676 2829 2893 3431 1937
Size: 739 153 64 538 4594
Fragments arranged by size:
4594 739 538 153 64
Acc65I G'GTAC_C
Cuts at: 3409 3409
Size: 6088
AclI AA'CG_TT
Cuts at: 1563 4215 4588 1563
Size: 2652 373 3063
Fragments arranged by size:
3063 2652 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2799 4151 5535 5803 1632
Size: 1167 1352 1384 268 1917
Fragments arranged by size:
1917 1384 1352 1167 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5891
AflIII A'CryG_T
Cuts at: 1203 4987 5964 1203
Size: 3784 977 1327
Fragments arranged by size:
3784 1327 977
AgeI A'CCGG_T
Cuts at: 4919 4919
Size: 6088
AhdI GACnn_n'nnGTC
Cuts at: 4817 4817
Size: 6088
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6088
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6088
AlwNI CAG_nnn'CTG
Cuts at: 3831 5403 3831
Size: 1572 4516
ApaLI G'TGCA_C
Cuts at: 2384 4145 5301 2384
Size: 1761 1156 3171
Fragments arranged by size:
3171 1761 1156
ApoI r'AATT_y
Cuts at: 1493 2408 3397 3623 3775 5884 1493
Size: 915 989 226 152 2109 1697
Fragments arranged by size:
2109 1697 989 915 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3080 4642 160
Size: 872 2048 1562 1606
Fragments arranged by size:
2048 1606 1562 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
AvaI C'yCGr_G
Cuts at: 1870 1943 2593 3413 1870
Size: 73 650 820 4545
Fragments arranged by size:
4545 820 650 73
BamHI G'GATC_C
Cuts at: 3418 3785 3418
Size: 367 5721
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2498 3349 3409 4864 618
Size: 325 217 627 711 851 60 1455 1842
Fragments arranged by size:
1842 1455 851 711 627 325 217 60
BanII G_rGCy'C
Cuts at: 1391 3407 1391
Size: 2016 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2805 928
Size: 971 906 4211
Fragments arranged by size:
4211 971 906
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4279 4313 1903
Size: 34 2342 34 3678
Fragments arranged by size:
3678 2342 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2055 3980 5190 2055
Size: 1925 1210 2953
Fragments arranged by size:
2953 1925 1210
BclI T'GATC_A
Cuts at: 1548 2346 1548
Size: 798 5290
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4699 136
Size: 107 122 71 631 605 3027 1525
Fragments arranged by size:
3027 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2795 6083 2795
Size: 3288 2800
BlpI GC'TnA_GC
Cuts at: 3505 3505
Size: 6088
Bme1580I G_kGCm'C
Cuts at: 1790 2086 2388 4149 5305 1790
Size: 296 302 1761 1156 2573
Fragments arranged by size:
2573 1761 1156 302 296
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6088
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3819 4777 442
Size: 416 53 83 399 24 2402 958 1753
Fragments arranged by size:
2402 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6088
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3137 4748 1239
Size: 1898 1611 2579
Fragments arranged by size:
2579 1898 1611
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2952 4213 5078 5376 2952
Size: 1261 865 298 3664
Fragments arranged by size:
3664 1261 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4751 882
Size: 617 3252 2219
Fragments arranged by size:
3252 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3385 3879 493
Size: 711 2181 494 2702
Fragments arranged by size:
2702 2181 711 494
BsaBI GATnn'nnATC
Cuts at: 3320 6082 3320
Size: 2762 3326
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3895 4277 4912 275
Size: 53 83 186 936 2362 382 635 1451
Fragments arranged by size:
2362 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4521 4919 5193 5340 5751 4521
Size: 398 274 147 411 4858
Fragments arranged by size:
4858 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2160 2190 1178
Size: 30 165 30 757 30 5076
Fragments arranged by size:
5076 757 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2726 1186
Size: 1540 4548
BseYI C'CCAG_C
Cuts at: 5291 5787 5291
Size: 496 5592
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5515
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3538 4299 5327 1540
Size: 275 186 1537 761 1028 2301
Fragments arranged by size:
2301 1537 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2048 2388 4149 4234 5305 2048
Size: 340 1761 85 1071 2831
Fragments arranged by size:
2831 1761 1071 340 85
BsmI GAATG_Cn'
Cuts at: 2441 3599 3692 2441
Size: 1158 93 4837
Fragments arranged by size:
4837 1158 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2048 2086 2388 3407 4149 4234 5305
Size: 399 258 38 302 1019 742 85 1071
Cuts at: 5305 1391
Size: 2174
Fragments arranged by size:
2174 1071 1019 742 399 302 258 85
38
BspEI T'CCGG_A
Cuts at: 5751 5751
Size: 6088
BspHI T'CATG_A
Cuts at: 3222 3977 5707 3222
Size: 755 1730 3603
Fragments arranged by size:
3603 1730 755
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2486 3443 844
Size: 1642 957 3489
Fragments arranged by size:
3489 1642 957
BsrBI CCG'CTC
Cuts at: 1394 1823 3975 1394
Size: 429 2152 3507
Fragments arranged by size:
3507 2152 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4583 4757 65
Size: 1183 3335 174 1396
Fragments arranged by size:
3335 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4732 4919 1700
Size: 110 2922 187 2869
Fragments arranged by size:
2922 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6088
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6088
BssSI C'ACGA_G
Cuts at: 3261 3344 4148 5160 3261
Size: 83 804 1012 4189
Fragments arranged by size:
4189 1012 804 83
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2423 794
Size: 1629 4459
BstBI TT'CG_AA
Cuts at: 2007 4892 2007
Size: 2885 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6088
BstYI r'GATC_y
Cuts at: 1972 2795 3418 3785 4170 4187 5639 5725 5737
Size: 823 623 367 385 17 1452 86 12
Cuts at: 5737 6083 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 823 623 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2049 513
Size: 552 957 27 4552
Fragments arranged by size:
4552 957 552 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5349
Fragments arranged by size:
5349 683 56
BtsI GCAGTG_nn'
Cuts at: 2371 2388 3604 4398 4418 2371
Size: 17 1216 794 20 4041
Fragments arranged by size:
4041 1216 794 20 17
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6088
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6053
DraI TTT'AAA
Cuts at: 2407 3392 2407
Size: 985 5103
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5095 809
Size: 507 3779 1802
Fragments arranged by size:
3779 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3535 4424 6053
Size: 54 1425 50 275 18 1705 889 1629
Cuts at: 6053 8
Size: 43
Fragments arranged by size:
1705 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3535 1537
Size: 275 1723 4090
Fragments arranged by size:
4090 1723 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2351 3310 4018 1353
Size: 998 959 708 3423
Fragments arranged by size:
3423 998 959 708
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4675 5049 5195 387
Size: 1064 138 3086 374 146 1280
Fragments arranged by size:
3086 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2799 3137 4151 4748 5535 5803 1239
Size: 393 1167 338 1014 597 787 268 1524
Fragments arranged by size:
1524 1167 1014 787 597 393 338 268
EcoRI G'AATT_C
Cuts at: 3397 3775 3397
Size: 378 5710
FspI TGC'GCA
Cuts at: 4594 4594
Size: 6088
HaeII r_GCGC'y
Cuts at: 1678 5235 1678
Size: 3557 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2973 3005 3259 3291 4729
Size: 32 135 32 1401 32 254 32 1438
Cuts at: 4729 4761 4803 4835 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1438 1401 254 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2728 3432 3678 669
Size: 988 281 790 704 246 3079
Fragments arranged by size:
3079 988 790 704 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6088
HpaI GTT'AAC
Cuts at: 3678 3678
Size: 6088
KpnI G_GTAC'C
Cuts at: 3413 3413
Size: 6088
MfeI C'AATT_G
Cuts at: 2421 3687 2421
Size: 1266 4822
MluI A'CGCG_T
Cuts at: 5964 5964
Size: 6088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2146 3293 5202 5386 1305
Size: 841 1147 1909 184 2007
Fragments arranged by size:
2007 1909 1147 841 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4266
Fragments arranged by size:
4266 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2071 2551 4046 4405 4564 518
Size: 648 905 480 1495 359 159 2042
Fragments arranged by size:
2042 1495 905 648 480 359 159
MspA1I CmG'CkG
Cuts at: 1606 3287 3504 4181 5329 5574 1606
Size: 1681 217 677 1148 245 2120
Fragments arranged by size:
2120 1681 1148 677 245 217
NcoI C'CATG_G
Cuts at: 513 1065 2022 2049 513
Size: 552 957 27 4552
Fragments arranged by size:
4552 957 552 27
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6088
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6088
NotI GC'GGCC_GC
Cuts at: 3535 3535
Size: 6088
NspI r_CATG'y
Cuts at: 1132 1229 2734 2966 3446 4991 1132
Size: 97 1505 232 480 1545 2229
Fragments arranged by size:
2229 1545 1505 480 232 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6088
PciI A'CATG_T
Cuts at: 4987 4987
Size: 6088
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2798 1108
Size: 1690 4398
PfoI T'CCnGG_A
Cuts at: 2227 2227
Size: 6088
PmeI GTTT'AAAC
Cuts at: 3392 3392
Size: 6088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4157 4189 5670 5702 4157
Size: 32 1481 32 4543
Fragments arranged by size:
4543 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3287 1613
Size: 1674 4414
PsiI TTA'TAA
Cuts at: 3658 3658
Size: 6088
PstI C_TGCA'G
Cuts at: 830 2383 2419 3440 830
Size: 1553 36 1021 3478
Fragments arranged by size:
3478 1553 1021 36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6088
PvuII CAG'CTG
Cuts at: 3287 3287
Size: 6088
SalI G'TCGA_C
Cuts at: 1936 3430 1936
Size: 1494 4594
SbfI CC_TGCA'GG
Cuts at: 3440 3440
Size: 6088
ScaI AGT'ACT
Cuts at: 4336 6048 4336
Size: 1712 4376
SfcI C'TryA_G
Cuts at: 826 1045 2379 2415 2625 3144 3436 4571 5252
Size: 219 1334 36 210 519 292 1135 681
Cuts at: 5252 5443 826
Size: 191 1471
Fragments arranged by size:
1471 1334 1135 681 519 292 219 210
191 36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6088
SmaI CCC'GGG
Cuts at: 1872 3415 1872
Size: 1543 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2593 2967 4192 5093 5355 820
Size: 197 926 650 374 1225 901 262 1553
Fragments arranged by size:
1553 1225 926 901 650 374 262 197
SnaBI TAC'GTA
Cuts at: 493 3385 3879 493
Size: 2892 494 2702
Fragments arranged by size:
2892 2702 494
SpeI A'CTAG_T
Cuts at: 152 2524 152
Size: 2372 3716
SphI G_CATG'C
Cuts at: 1132 2966 3446 1132
Size: 1834 480 3774
Fragments arranged by size:
3774 1834 480
SspI AAT'ATT
Cuts at: 5 52 4012 5
Size: 47 3960 2081
Fragments arranged by size:
3960 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6088
StyI C'CwwG_G
Cuts at: 513 1065 2022 2049 3527 513
Size: 552 957 27 1478 3074
Fragments arranged by size:
3074 1478 957 552 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3283 4115 4132 4285 4470 4915 325
Size: 1255 1703 832 17 153 185 445 1498
Fragments arranged by size:
1703 1498 1255 832 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4334 6046 96
Size: 274 80 33 51 1443 2357 1712 138
Fragments arranged by size:
2357 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6048 6080 6048
Size: 32 6056
Tth111I GACn'n_nGTC
Cuts at: 1316 2848 1316
Size: 1532 4556
XbaI T'CTAG_A
Cuts at: 3424 3424
Size: 6088
XhoI C'TCGA_G
Cuts at: 1943 2593 1943
Size: 650 5438
XmaI C'CCGG_G
Cuts at: 1870 3413 1870
Size: 1543 4545
XmnI GAAnn'nnTTC
Cuts at: 4217 4217
Size: 6088
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3896 276
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI