Restriction map for
pFN21ASDA1247
(Circular) (Six-base) MAPSORT of: pFN21ASDA1247.seq Check: 1224 from: 1 to: 7339
pFN21ASDA1247 7339 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2197 2197
Size: 7339
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5149 278
Size: 53 83 186 936 3613 2468
Fragments arranged by size:
3613 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2961 4682 1937
Size: 1024 1721 4594
Fragments arranged by size:
4594 1721 1024
Acc65I G'GTAC_C
Cuts at: 4660 4660
Size: 7339
AclI AA'CG_TT
Cuts at: 1563 5466 5839 1563
Size: 3903 373 3063
Fragments arranged by size:
3903 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3458 3751 3998 5402 6786 7054 1632
Size: 1826 293 247 1404 1384 268 1917
Fragments arranged by size:
1917 1826 1404 1384 293 268 247
AfeI AGC'GCT
Cuts at: 2870 2870
Size: 7339
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7142
AflIII A'CryG_T
Cuts at: 1203 2949 4176 4287 4441 6238 7215 1203
Size: 1746 1227 111 154 1797 977 1327
Fragments arranged by size:
1797 1746 1327 1227 977 154 111
AgeI A'CCGG_T
Cuts at: 6170 6170
Size: 7339
AhdI GACnn_n'nnGTC
Cuts at: 6068 6068
Size: 7339
AleI CACnn'nnGTG
Cuts at: 1166 2281 3826 1166
Size: 1115 1545 4679
Fragments arranged by size:
4679 1545 1115
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7339
AlwNI CAG_nnn'CTG
Cuts at: 5082 6654 5082
Size: 1572 5767
ApaI G_GGCC'C
Cuts at: 2030 2823 3065 3461 3733 2030
Size: 793 242 396 272 5636
Fragments arranged by size:
5636 793 396 272 242
ApaLI G'TGCA_C
Cuts at: 5396 6552 5396
Size: 1156 6183
ApoI r'AATT_y
Cuts at: 1493 2843 4340 4648 4874 5026 7135 1493
Size: 1350 1497 308 226 152 2109 1697
Fragments arranged by size:
2109 1697 1497 1350 308 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5893 160
Size: 872 4861 1606
Fragments arranged by size:
4861 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7339
AvaI C'yCGr_G
Cuts at: 1870 1943 2032 4664 1870
Size: 73 89 2632 4545
Fragments arranged by size:
4545 2632 89 73
BamHI G'GATC_C
Cuts at: 4669 5036 4669
Size: 367 6972
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4234 4660 6115 618
Size: 325 217 627 2447 426 1455 1842
Fragments arranged by size:
2447 1842 1455 627 426 325 217
BanII G_rGCy'C
Cuts at: 1391 2030 2823 3065 3461 3487 3733 4658 1391
Size: 639 793 242 396 26 246 925 4072
Fragments arranged by size:
4072 925 793 639 396 246 242 26
BbeI G_GCGC'C
Cuts at: 4238 4238
Size: 7339
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6368
BbvCI CC'TCA_GC
Cuts at: 4460 4460
Size: 7339
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2023 2057 5530 5564 1903
Size: 34 86 34 3473 34 3678
Fragments arranged by size:
3678 3473 86 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5231 6441 5231
Size: 1210 6129
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7339
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2118 2374 2997
Size: 107 122 71 631 605 446 256 623
Cuts at: 2997 5950 136
Size: 2953 1525
Fragments arranged by size:
2953 1525 631 623 605 446 256 122
107 71
BglII A'GATC_T
Cuts at: 7334 7334
Size: 7339
BlpI GC'TnA_GC
Cuts at: 3560 4756 3560
Size: 1196 6143
Bme1580I G_kGCm'C
Cuts at: 1790 2030 2823 3065 3461 3504 3733 5400 6556
Size: 240 793 242 396 43 229 1667 1156
Cuts at: 6556 1790
Size: 2573
Fragments arranged by size:
2573 1667 1156 793 396 242 240 229
43
BmgBI CAC'GTC
Cuts at: 1318 2640 1318
Size: 1322 6017
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4259 4613 5070
Size: 416 53 83 399 24 2842 354 457
Cuts at: 5070 6028 442
Size: 958 1753
Fragments arranged by size:
2842 1753 958 457 416 399 354 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7339
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2377 2377
Size: 7339
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2843 3798 4045 5999 1239
Size: 1604 955 247 1954 2579
Fragments arranged by size:
2579 1954 1604 955 247
Bpu10I CC'TnA_GC
Cuts at: 4460 4460
Size: 7339
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3167 4031 4139 5464 6329 6627 3167
Size: 864 108 1325 865 298 3879
Fragments arranged by size:
3879 1325 865 864 298 108
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4579 6002 882
Size: 617 3080 1423 2219
Fragments arranged by size:
3080 2219 1423 617
BsaAI yAC'GTr
Cuts at: 493 1204 2479 2952 3055 3673 4179 4200 4290
Size: 711 1275 473 103 618 506 21 90
Cuts at: 4290 4444 4636 5130 493
Size: 154 192 494 2702
Fragments arranged by size:
2702 1275 711 618 506 494 473 192
154 103 90 21
BsaBI GATnn'nnATC
Cuts at: 7333 7333
Size: 7339
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3309 3423 3574 4235
Size: 53 83 186 936 1776 114 151 661
Cuts at: 4235 5146 5528 6163 275
Size: 911 382 635 1451
Fragments arranged by size:
1776 1451 936 911 661 635 382 186
151 114 83 53
BsaWI w'CCGG_w
Cuts at: 2430 5772 6170 6444 6591 7002 2430
Size: 3342 398 274 147 411 2767
Fragments arranged by size:
3342 2767 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2456 2486 4043 4073 1178
Size: 30 165 30 1053 30 1557 30 4444
Fragments arranged by size:
4444 1557 1053 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2290 2353 3332 4477 1186
Size: 1104 63 979 1145 4048
Fragments arranged by size:
4048 1145 1104 979 63
BseYI C'CCAG_C
Cuts at: 2077 2194 3394 3469 3693 4029 4623 6542 7038
Size: 117 1200 75 224 336 594 1919 496
Cuts at: 7038 2077
Size: 2378
Fragments arranged by size:
2378 1919 1200 594 496 336 224 117
75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3517 3893 4201 4252 1163
Size: 573 1781 376 308 51 4250
Fragments arranged by size:
4250 1781 573 376 308 51
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3206 4789 5550 6578 1540
Size: 275 186 1205 1583 761 1028 2301
Fragments arranged by size:
2301 1583 1205 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5400 5485 6556 5400
Size: 85 1071 6183
Fragments arranged by size:
6183 1071 85
BsmI GAATG_Cn'
Cuts at: 4405 4850 4943 4405
Size: 445 93 6801
Fragments arranged by size:
6801 445 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2900 2924 2900
Size: 24 7315
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2823 3065 3461 3487 3504 3733
Size: 399 240 793 242 396 26 17 229
Cuts at: 3733 4658 5400 5485 6556 1391
Size: 925 742 85 1071 2174
Fragments arranged by size:
2174 1071 925 793 742 399 396 242
240 229 85 26 17
BspEI T'CCGG_A
Cuts at: 7002 7002
Size: 7339
BspHI T'CATG_A
Cuts at: 5228 6958 5228
Size: 1730 5609
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2112 2197 3163 4694 844
Size: 1268 85 966 1531 3489
Fragments arranged by size:
3489 1531 1268 966 85
BsrBI CCG'CTC
Cuts at: 1394 1823 3202 3636 5226 1394
Size: 429 1379 434 1590 3507
Fragments arranged by size:
3507 1590 1379 434 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3135 5834 6008 65
Size: 1183 1887 2699 174 1396
Fragments arranged by size:
2699 1887 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2312 3077 3205 5983 6170 1700
Size: 110 502 765 128 2778 187 2869
Fragments arranged by size:
2869 2778 765 502 187 128 110
BsrGI T'GTAC_A
Cuts at: 96 3873 96
Size: 3777 3562
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7339
BssSI C'ACGA_G
Cuts at: 2039 2387 3497 3920 5399 6411 2039
Size: 348 1110 423 1479 1012 2967
Fragments arranged by size:
2967 1479 1110 1012 423 348
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7339
BstBI TT'CG_AA
Cuts at: 2007 6143 2007
Size: 4136 3203
BstEII G'GTnAC_C
Cuts at: 1184 2978 3971 1184
Size: 1794 993 4552
Fragments arranged by size:
4552 1794 993
BstXI CCAn_nnnn'nTGG
Cuts at: 2490 2839 3130 3469 3652 4603 2490
Size: 349 291 339 183 951 5226
Fragments arranged by size:
5226 951 349 339 291 183
BstYI r'GATC_y
Cuts at: 1972 4669 5036 5421 5438 6890 6976 6988 7334
Size: 2697 367 385 17 1452 86 12 346
Cuts at: 7334 1972
Size: 1977
Fragments arranged by size:
2697 1977 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2962 2962
Size: 7339
Bsu36I CC'TnA_GG
Cuts at: 2706 3735 3956 2706
Size: 1029 221 6089
Fragments arranged by size:
6089 1029 221
BtgI C'CryG_G
Cuts at: 513 1065 2022 2698 2901 2974 3426 513
Size: 552 957 676 203 73 452 4426
Fragments arranged by size:
4426 957 676 552 452 203 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6600
Fragments arranged by size:
6600 683 56
BtsI GCAGTG_nn'
Cuts at: 3416 4410 4490 4855 5649 5669 3416
Size: 994 80 365 794 20 5086
Fragments arranged by size:
5086 994 794 365 80 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7339
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3739 3774 529
Size: 35 3175 35 4094
Fragments arranged by size:
4094 3175 35 35
DraI TTT'AAA
Cuts at: 4643 4643
Size: 7339
DraIII CAC_nnn'GTG
Cuts at: 2544 2544
Size: 7339
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2884 3041 4424 6346 809
Size: 507 1568 157 1383 1922 1802
Fragments arranged by size:
1922 1802 1568 1383 507 157
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2989 3203 3567
Size: 54 1425 50 275 18 1159 214 364
Cuts at: 3567 3642 3722 3989 4571 4786 5675 7304 8
Size: 75 80 267 582 215 889 1629 43
Fragments arranged by size:
1629 1425 1159 889 582 364 275 267
215 214 80 75 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3203 4786 1537
Size: 275 1391 1583 4090
Fragments arranged by size:
4090 1583 1391 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2741 3593 4337 4577 5269 1353
Size: 1388 852 744 240 692 3423
Fragments arranged by size:
3423 1388 852 744 692 240
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2138 5926 6300 6446 387
Size: 1064 138 549 3788 374 146 1280
Fragments arranged by size:
3788 1280 1064 549 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2843 3458 3751 3798 3998 4045 5402
Size: 393 1211 615 293 47 200 47 1357
Cuts at: 5402 5999 6786 7054 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1357 1211 787 615 597 393 293
268 200 47 47
EcoO109I rG'GnC_Cy
Cuts at: 2027 2819 3043 3061 3062 3352 3457 3458 3729
Size: 792 224 18 1 290 105 1 271
Cuts at: 3729 3730 4084 4253 2027
Size: 1 354 169 5113
Fragments arranged by size:
5113 792 354 290 271 224 169 105
18 1 1 1
EcoRI G'AATT_C
Cuts at: 2843 4648 5026 2843
Size: 1805 378 5156
Fragments arranged by size:
5156 1805 378
FspI TGC'GCA
Cuts at: 5845 5845
Size: 7339
HaeII r_GCGC'y
Cuts at: 1678 2872 4162 4238 4278 6486 1678
Size: 1194 1290 76 40 2208 2531
Fragments arranged by size:
2531 2208 1290 1194 76 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3158 3190 5980 6012 6054
Size: 32 135 32 1586 32 2790 32 42
Cuts at: 6054 6086 1373
Size: 32 2626
Fragments arranged by size:
2790 2626 1586 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4683 4929 669
Size: 988 281 2745 246 3079
Fragments arranged by size:
3079 2745 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7339
HpaI GTT'AAC
Cuts at: 4929 4929
Size: 7339
KasI G'GCGC_C
Cuts at: 4234 4234
Size: 7339
KpnI G_GTAC'C
Cuts at: 4664 4664
Size: 7339
MfeI C'AATT_G
Cuts at: 4938 4938
Size: 7339
MluI A'CGCG_T
Cuts at: 7215 7215
Size: 7339
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2485 2528 2601 3516 6453 6637 1305
Size: 1180 43 73 915 2937 184 2007
Fragments arranged by size:
2937 2007 1180 915 184 73 43
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3644 3724 4573 10
Size: 54 1425 343 1812 80 849 2776
Fragments arranged by size:
2776 1812 1425 849 343 80 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2165 2207 2279 2281 2837 2852 2888
Size: 648 999 42 72 2 556 15 36
Cuts at: 2888 3650 3815 3826 5297 5656 5815 518
Size: 762 165 11 1471 359 159 2042
Fragments arranged by size:
2042 1471 999 762 648 556 359 165
159 72 42 36 15 11 2
MspA1I CmG'CkG
Cuts at: 1606 2863 3394 3546 4393 4755 5432 6580 6825
Size: 1257 531 152 847 362 677 1148 245
Cuts at: 6825 1606
Size: 2120
Fragments arranged by size:
2120 1257 1148 847 677 531 362 245
152
NaeI GCC'GGC
Cuts at: 3079 3079
Size: 7339
NarI GG'CG_CC
Cuts at: 4235 4235
Size: 7339
NcoI C'CATG_G
Cuts at: 513 1065 2022 2901 513
Size: 552 957 879 4951
Fragments arranged by size:
4951 957 879 552
NdeI CA'TA_TG
Cuts at: 387 3025 387
Size: 2638 4701
NgoMIV G'CCGG_C
Cuts at: 3077 3077
Size: 7339
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7339
NotI GC'GGCC_GC
Cuts at: 4786 4786
Size: 7339
NspI r_CATG'y
Cuts at: 1132 1229 2857 2923 4189 4697 6242 1132
Size: 97 1628 66 1266 508 1545 2229
Fragments arranged by size:
2229 1628 1545 1266 508 97 66
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7339
PciI A'CATG_T
Cuts at: 6238 6238
Size: 7339
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2188 1108
Size: 1080 6259
PfoI T'CCnGG_A
Cuts at: 3129 3129
Size: 7339
PmeI GTTT'AAAC
Cuts at: 4643 4643
Size: 7339
PmlI CAC'GTG
Cuts at: 4179 4200 4179
Size: 21 7318
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2348 2380 5408 5440 6921 6953 2348
Size: 32 3028 32 1481 32 2734
Fragments arranged by size:
3028 2734 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3043 3352 3043
Size: 309 7030
PshAI GACnn'nnGTC
Cuts at: 1613 3357 1613
Size: 1744 5595
PsiI TTA'TAA
Cuts at: 2532 4909 2532
Size: 2377 4962
PspOMI G'GGCC_C
Cuts at: 2026 2819 3061 3457 3729 2026
Size: 793 242 396 272 5636
Fragments arranged by size:
5636 793 396 272 242
PstI C_TGCA'G
Cuts at: 830 3888 3939 4167 4691 830
Size: 3058 51 228 524 3478
Fragments arranged by size:
3478 3058 524 228 51
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7339
PvuII CAG'CTG
Cuts at: 3394 4393 3394
Size: 999 6340
SalI G'TCGA_C
Cuts at: 1936 4681 1936
Size: 2745 4594
SanDI GG'GwC_CC
Cuts at: 3043 3043
Size: 7339
SbfI CC_TGCA'GG
Cuts at: 3888 4691 3888
Size: 803 6536
ScaI AGT'ACT
Cuts at: 3509 4364 5587 7299 3509
Size: 855 1223 1712 3549
Fragments arranged by size:
3549 1712 1223 855
SfcI C'TryA_G
Cuts at: 826 1045 3884 3935 4163 4687 5822 6503 6694
Size: 219 2839 51 228 524 1135 681 191
Cuts at: 6694 826
Size: 1471
Fragments arranged by size:
2839 1471 1135 681 524 228 219 191
51
SfiI GGCCn_nnn'nGGCC
Cuts at: 2997 2997
Size: 7339
SfoI GGC'GCC
Cuts at: 4236 4236
Size: 7339
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7339
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7339
SmaI CCC'GGG
Cuts at: 1872 4666 1872
Size: 2794 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3182 4046 4154 5443 6344 6606
Size: 197 926 1239 864 108 1289 901 262
Cuts at: 6606 820
Size: 1553
Fragments arranged by size:
1553 1289 1239 926 901 864 262 197
108
SnaBI TAC'GTA
Cuts at: 493 4636 5130 493
Size: 4143 494 2702
Fragments arranged by size:
4143 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7339
SphI G_CATG'C
Cuts at: 1132 4189 4697 1132
Size: 3057 508 3774
Fragments arranged by size:
3774 3057 508
SspI AAT'ATT
Cuts at: 5 52 2726 5263 5
Size: 47 2674 2537 2081
Fragments arranged by size:
2674 2537 2081 47
StuI AGG'CCT
Cuts at: 1517 3144 3530 3678 3783 3890 4194 1517
Size: 1627 386 148 105 107 304 4662
Fragments arranged by size:
4662 1627 386 304 148 107 105
StyI C'CwwG_G
Cuts at: 513 1065 2022 2586 2901 4778 513
Size: 552 957 564 315 1877 3074
Fragments arranged by size:
3074 1877 957 564 552 315
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2069 2293 3671 4043 4223 5366 5383
Size: 1255 489 224 1378 372 180 1143 17
Cuts at: 5383 5536 5721 6166 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1378 1255 1143 489 445 372 224
185 180 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3507 3600 3873
Size: 274 80 33 51 1443 1530 93 273
Cuts at: 3873 4362 5585 7297 96
Size: 489 1223 1712 138
Fragments arranged by size:
1712 1530 1443 1223 489 274 273 138
93 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2721 2753 4043 4075 4091 4123 7299 7331 2721
Size: 32 1290 32 16 32 3176 32 2729
Fragments arranged by size:
3176 2729 1290 32 32 32 32 16
Tth111I GACn'n_nGTC
Cuts at: 1316 3851 1316
Size: 2535 4804
XbaI T'CTAG_A
Cuts at: 4675 4675
Size: 7339
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2161 3637 2161
Size: 1476 5863
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7339
XmaI C'CCGG_G
Cuts at: 1870 4664 1870
Size: 2794 4545
XmnI GAAnn'nnTTC
Cuts at: 5468 5468
Size: 7339
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5147 276
Size: 53 83 186 936 3613 2468
Fragments arranged by size:
3613 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI EcoICRI EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PspXI
PsrI RsrII SacI SacII SapI SexAI SrfI SwaI