Restriction map for
pFN21ASDA1246
(Circular) (Six-base) MAPSORT of: pFN21ASDA1246.seq Check: 3570 from: 1 to: 7096
pFN21ASDA1246 7096 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3104 3104
Size: 7096
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4082 4906 278
Size: 53 83 186 936 2546 824 2468
Fragments arranged by size:
2546 2468 936 824 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2076 4389 4439 1937
Size: 139 2313 50 4594
Fragments arranged by size:
4594 2313 139 50
Acc65I G'GTAC_C
Cuts at: 4417 4417
Size: 7096
AclI AA'CG_TT
Cuts at: 1563 5223 5596 1563
Size: 3660 373 3063
Fragments arranged by size:
3660 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3330 5159 6543 6811 1632
Size: 1698 1829 1384 268 1917
Fragments arranged by size:
1917 1829 1698 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6899
AflIII A'CryG_T
Cuts at: 1203 5995 6972 1203
Size: 4792 977 1327
Fragments arranged by size:
4792 1327 977
AgeI A'CCGG_T
Cuts at: 5927 5927
Size: 7096
AhdI GACnn_n'nnGTC
Cuts at: 2537 5825 2537
Size: 3288 3808
AleI CACnn'nnGTG
Cuts at: 1166 2947 1166
Size: 1781 5315
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2372 4328 1184
Size: 1188 1956 3952
Fragments arranged by size:
3952 1956 1188
AlwNI CAG_nnn'CTG
Cuts at: 2110 2617 2656 3476 3610 4839 6411 2110
Size: 507 39 820 134 1229 1572 2795
Fragments arranged by size:
2795 1572 1229 820 507 134 39
ApaI G_GGCC'C
Cuts at: 3805 3818 3937 4125 4345 3805
Size: 13 119 188 220 6556
Fragments arranged by size:
6556 220 188 119 13
ApaLI G'TGCA_C
Cuts at: 5153 6309 5153
Size: 1156 5940
ApoI r'AATT_y
Cuts at: 1493 4405 4631 4783 6892 1493
Size: 2912 226 152 2109 1697
Fragments arranged by size:
2912 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5650 160
Size: 872 4618 1606
Fragments arranged by size:
4618 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7096
AvaI C'yCGr_G
Cuts at: 1870 1943 2318 2789 3363 4086 4134 4344 4421
Size: 73 375 471 574 723 48 210 77
Cuts at: 4421 1870
Size: 4545
Fragments arranged by size:
4545 723 574 471 375 210 77 73
48
BamHI G'GATC_C
Cuts at: 3945 4426 4793 3945
Size: 481 367 6248
Fragments arranged by size:
6248 481 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2594 3047 3086 3617 3764
Size: 325 217 627 807 453 39 531 147
Cuts at: 3764 4417 5872 618
Size: 653 1455 1842
Fragments arranged by size:
1842 1455 807 653 627 531 453 325
217 147 39
BanII G_rGCy'C
Cuts at: 1391 3805 3818 3846 3937 4125 4345 4415 1391
Size: 2414 13 28 91 188 220 70 4072
Fragments arranged by size:
4072 2414 220 188 91 70 28 13
BbeI G_GCGC'C
Cuts at: 3768 3768
Size: 7096
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3658 928
Size: 971 1759 4366
Fragments arranged by size:
4366 1759 971
BbvCI CC'TCA_GC
Cuts at: 3167 3167
Size: 7096
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5287 5321 1903
Size: 34 3350 34 3678
Fragments arranged by size:
3678 3350 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4988 6198 4988
Size: 1210 5886
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7096
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2202 3122 3866
Size: 107 122 71 631 605 530 920 744
Cuts at: 3866 5707 136
Size: 1841 1525
Fragments arranged by size:
1841 1525 920 744 631 605 530 122
107 71
BglII A'GATC_T
Cuts at: 7091 7091
Size: 7096
BlpI GC'TnA_GC
Cuts at: 2555 4513 2555
Size: 1958 5138
Bme1580I G_kGCm'C
Cuts at: 1790 2138 2597 3805 3818 3937 4125 4345 5157
Size: 348 459 1208 13 119 188 220 812
Cuts at: 5157 6313 1790
Size: 1156 2573
Fragments arranged by size:
2573 1208 1156 812 459 348 220 188
119 13
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7096
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2132 2995 3550
Size: 416 53 83 399 24 715 863 555
Cuts at: 3550 4827 5785 442
Size: 1277 958 1753
Fragments arranged by size:
1753 1277 958 863 715 555 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2051 1056
Size: 995 6101
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2945 3211 3877 3961 4033 4041 5756 1239
Size: 1706 266 666 84 72 8 1715 2579
Fragments arranged by size:
2579 1715 1706 666 266 84 72 8
Bpu10I CC'TnA_GC
Cuts at: 3167 3167
Size: 7096
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2819 2930 3007 4001 5221 6086 6384 2819
Size: 111 77 994 1220 865 298 3531
Fragments arranged by size:
3531 1220 994 865 298 111 77
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2021 3148 4270 5759 882
Size: 617 522 1127 1122 1489 2219
Fragments arranged by size:
2219 1489 1127 1122 617 522
BsaAI yAC'GTr
Cuts at: 493 1204 4393 4887 493
Size: 711 3189 494 2702
Fragments arranged by size:
3189 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7090 7090
Size: 7096
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2079 3765 4079 4903
Size: 53 83 186 936 546 1686 314 824
Cuts at: 4903 5285 5920 275
Size: 382 635 1451
Fragments arranged by size:
1686 1451 936 824 635 546 382 314
186 83 53
BsaWI w'CCGG_w
Cuts at: 5529 5927 6201 6348 6759 5529
Size: 398 274 147 411 5866
Fragments arranged by size:
5866 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2861 2891 4218 4248 1178
Size: 30 165 30 1458 30 1327 30 4026
Fragments arranged by size:
4026 1458 1327 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2879 2882 3010 3874 4049 4245 4337 1186
Size: 1693 3 128 864 175 196 92 3945
Fragments arranged by size:
3945 1693 864 196 175 128 92 3
BseYI C'CCAG_C
Cuts at: 2428 2898 2969 3430 3716 3768 3925 3974 4073
Size: 470 71 461 286 52 157 49 99
Cuts at: 4073 4117 6299 6795 2428
Size: 44 2182 496 2729
Fragments arranged by size:
2729 2182 496 470 461 286 157 99
71 52 49 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3174 1163
Size: 573 1438 5085
Fragments arranged by size:
5085 1438 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3735 4546 5307 6335 1540
Size: 275 186 1734 811 761 1028 2301
Fragments arranged by size:
2301 1734 1028 811 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3846 4385 5157 5242 6313 3846
Size: 539 772 85 1071 4629
Fragments arranged by size:
4629 1071 772 539 85
BsmI GAATG_Cn'
Cuts at: 4607 4700 4607
Size: 93 7003
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2138 2597 3805 3818 3846 3937 4125
Size: 399 348 459 1208 13 28 91 188
Cuts at: 4125 4345 4385 4415 5157 5242 6313 1391
Size: 220 40 30 742 85 1071 2174
Fragments arranged by size:
2174 1208 1071 742 459 399 348 220
188 91 85 40 30 28 13
BspEI T'CCGG_A
Cuts at: 6759 6759
Size: 7096
BspHI T'CATG_A
Cuts at: 4985 6715 4985
Size: 1730 5366
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2406 2580 2616 3104 4451 844
Size: 1562 174 36 488 1347 3489
Fragments arranged by size:
3489 1562 1347 488 174 36
BsrBI CCG'CTC
Cuts at: 1394 1823 2331 2682 2794 4983 1394
Size: 429 508 351 112 2189 3507
Fragments arranged by size:
3507 2189 508 429 351 112
BsrDI GCAATG_nn'
Cuts at: 65 1248 3099 3748 5591 5765 65
Size: 1183 1851 649 1843 174 1396
Fragments arranged by size:
1851 1843 1396 1183 649 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2175 5740 5927 1700
Size: 110 365 3565 187 2869
Fragments arranged by size:
3565 2869 365 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7096
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7096
BssSI C'ACGA_G
Cuts at: 3707 5156 6168 3707
Size: 1449 1012 4635
Fragments arranged by size:
4635 1449 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2656 794
Size: 1862 5234
BstBI TT'CG_AA
Cuts at: 2007 5900 2007
Size: 3893 3203
BstEII G'GTnAC_C
Cuts at: 1184 2744 1184
Size: 1560 5536
BstXI CCAn_nnnn'nTGG
Cuts at: 3133 3544 3628 3661 3724 3901 3133
Size: 411 84 33 63 177 6328
Fragments arranged by size:
6328 411 177 84 63 33
BstYI r'GATC_y
Cuts at: 1972 2479 2629 3945 4426 4793 5178 5195 6647
Size: 507 150 1316 481 367 385 17 1452
Cuts at: 6647 6733 6745 7091 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1316 507 481 385 367 346
150 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2070 2506 2516 3126 3132 513
Size: 552 957 48 436 10 610 6 4477
Fragments arranged by size:
4477 957 610 552 436 48 10 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2422 2569 487
Size: 683 56 1196 147 5014
Fragments arranged by size:
5014 1196 683 147 56
BtsI GCAGTG_nn'
Cuts at: 2755 3186 3262 3590 3913 4112 4612 5406 5426
Size: 431 76 328 323 199 500 794 20
Cuts at: 5426 2755
Size: 4425
Fragments arranged by size:
4425 794 500 431 328 323 199 76
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7096
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2695 2730 529
Size: 35 2131 35 4895
Fragments arranged by size:
4895 2131 35 35
DraI TTT'AAA
Cuts at: 4400 4400
Size: 7096
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6103 809
Size: 507 4787 1802
Fragments arranged by size:
4787 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2937 3129 3498
Size: 54 1425 50 275 18 1107 192 369
Cuts at: 3498 3531 3651 3732 4543 5432 7061 8
Size: 33 120 81 811 889 1629 43
Fragments arranged by size:
1629 1425 1107 889 811 369 275 192
120 81 54 50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 3732 4543 1537
Size: 275 1920 811 4090
Fragments arranged by size:
4090 1920 811 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3892 5026 1353
Size: 2539 1134 3423
Fragments arranged by size:
3423 2539 1134
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2194 3279 3946 5683 6057 6203
Size: 1064 138 605 1085 667 1737 374 146
Cuts at: 6203 387
Size: 1280
Fragments arranged by size:
1737 1280 1085 1064 667 605 374 146
138
EcoICRI GAG'CTC
Cuts at: 3844 3844
Size: 7096
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2945 3211 3330 3877 3961 4033 4041
Size: 393 1313 266 119 547 84 72 8
Cuts at: 4041 5159 5756 6543 6811 1239
Size: 1118 597 787 268 1524
Fragments arranged by size:
1524 1313 1118 787 597 547 393 268
266 119 84 72 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2369 2369
Size: 7096
EcoO109I rG'GnC_Cy
Cuts at: 2127 2386 2830 2965 3712 3801 3802 3814 3934
Size: 259 444 135 747 89 1 12 120
Cuts at: 3934 3996 4121 4122 4341 2127
Size: 62 125 1 219 4882
Fragments arranged by size:
4882 747 444 259 219 135 125 120
89 62 12 1 1
EcoRI G'AATT_C
Cuts at: 4405 4783 4405
Size: 378 6718
FspI TGC'GCA
Cuts at: 3836 5602 3836
Size: 1766 5330
HaeII r_GCGC'y
Cuts at: 1678 3768 6243 1678
Size: 2090 2475 2531
Fragments arranged by size:
2531 2475 2090
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2523 2555 2621 2653 5737
Size: 32 135 32 951 32 66 32 3084
Cuts at: 5737 5769 5811 5843 1373
Size: 32 42 32 2626
Fragments arranged by size:
3084 2626 951 135 66 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2077 2395 2542 3457 4300 4440
Size: 988 281 139 318 147 915 843 140
Cuts at: 4440 4686 669
Size: 246 3079
Fragments arranged by size:
3079 988 915 843 318 281 246 147
140 139
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7096
HpaI GTT'AAC
Cuts at: 4686 4686
Size: 7096
KasI G'GCGC_C
Cuts at: 3764 3764
Size: 7096
KpnI G_GTAC'C
Cuts at: 4421 4421
Size: 7096
MfeI C'AATT_G
Cuts at: 2761 4695 2761
Size: 1934 5162
MluI A'CGCG_T
Cuts at: 6972 6972
Size: 7096
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3462 4143 6210 6394 1305
Size: 2157 681 2067 184 2007
Fragments arranged by size:
2157 2067 2007 681 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3500 3653 10
Size: 54 1425 343 1668 153 3453
Fragments arranged by size:
3453 1668 1425 343 153 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2859 2947 3131 3647 3680 5054 5413
Size: 648 1693 88 184 516 33 1374 359
Cuts at: 5413 5572 518
Size: 159 2042
Fragments arranged by size:
2042 1693 1374 648 516 359 184 159
88 33
MspA1I CmG'CkG
Cuts at: 1606 2428 2554 3032 3157 3382 3731 4512 5189
Size: 822 126 478 125 225 349 781 677
Cuts at: 5189 6337 6582 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 822 781 677 478 349 245
225 126 125
NarI GG'CG_CC
Cuts at: 3765 3765
Size: 7096
NcoI C'CATG_G
Cuts at: 513 1065 2022 2506 3132 513
Size: 552 957 484 626 4477
Fragments arranged by size:
4477 957 626 552 484
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7096
NheI G'CTAG_C
Cuts at: 1052 2047 1052
Size: 995 6101
NotI GC'GGCC_GC
Cuts at: 4543 4543
Size: 7096
NspI r_CATG'y
Cuts at: 1132 1229 2858 4309 4454 5999 1132
Size: 97 1629 1451 145 1545 2229
Fragments arranged by size:
2229 1629 1545 1451 145 97
PasI CC'CwG_GG
Cuts at: 1348 2825 3901 1348
Size: 1477 1076 4543
Fragments arranged by size:
4543 1477 1076
PciI A'CATG_T
Cuts at: 5995 5995
Size: 7096
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2366 3610 1108
Size: 1258 1244 4594
Fragments arranged by size:
4594 1258 1244
PfoI T'CCnGG_A
Cuts at: 2273 3846 4049 2273
Size: 1573 203 5320
Fragments arranged by size:
5320 1573 203
PmeI GTTT'AAAC
Cuts at: 4400 4400
Size: 7096
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5165 5197 6678 6710 5165
Size: 32 1481 32 5551
Fragments arranged by size:
5551 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2127 2965 2127
Size: 838 6258
PshAI GACnn'nnGTC
Cuts at: 1613 3032 1613
Size: 1419 5677
PsiI TTA'TAA
Cuts at: 4666 4666
Size: 7096
PspOMI G'GGCC_C
Cuts at: 3801 3814 3933 4121 4341 3801
Size: 13 119 188 220 6556
Fragments arranged by size:
6556 220 188 119 13
PspXI vC'TCGA_Gb
Cuts at: 2789 2789
Size: 7096
PstI C_TGCA'G
Cuts at: 830 2403 2613 2652 3387 4448 830
Size: 1573 210 39 735 1061 3478
Fragments arranged by size:
3478 1573 1061 735 210 39
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7096
PvuII CAG'CTG
Cuts at: 2428 2554 3157 3382 2428
Size: 126 603 225 6142
Fragments arranged by size:
6142 603 225 126
RsrII CG'GwC_CG
Cuts at: 4102 4102
Size: 7096
SacI G_AGCT'C
Cuts at: 3846 3846
Size: 7096
SalI G'TCGA_C
Cuts at: 1936 2075 4438 1936
Size: 139 2363 4594
Fragments arranged by size:
4594 2363 139
SanDI GG'GwC_CC
Cuts at: 2127 2965 2127
Size: 838 6258
SbfI CC_TGCA'GG
Cuts at: 4448 4448
Size: 7096
ScaI AGT'ACT
Cuts at: 2093 5344 7056 2093
Size: 3251 1712 2133
Fragments arranged by size:
3251 2133 1712
SexAI A'CCwGG_T
Cuts at: 3462 4332 3462
Size: 870 6226
SfcI C'TryA_G
Cuts at: 826 1045 2399 2609 2648 3383 4246 4444 5579
Size: 219 1354 210 39 735 863 198 1135
Cuts at: 5579 6260 6451 826
Size: 681 191 1471
Fragments arranged by size:
1471 1354 1135 863 735 681 219 210
198 191 39
SfoI GGC'GCC
Cuts at: 3766 3766
Size: 7096
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7096
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7096
SmaI CCC'GGG
Cuts at: 1872 3365 4088 4136 4346 4423 1872
Size: 1493 723 48 210 77 4545
Fragments arranged by size:
4545 1493 723 210 77 48
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2318 2789 2834 2945 3022 4016
Size: 197 926 375 471 45 111 77 994
Cuts at: 4016 5200 6101 6363 820
Size: 1184 901 262 1553
Fragments arranged by size:
1553 1184 994 926 901 471 375 262
197 111 77 45
SnaBI TAC'GTA
Cuts at: 493 4393 4887 493
Size: 3900 494 2702
Fragments arranged by size:
3900 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7096
SphI G_CATG'C
Cuts at: 1132 4309 4454 1132
Size: 3177 145 3774
Fragments arranged by size:
3774 3177 145
SrfI GCCC'GGGC
Cuts at: 4136 4136
Size: 7096
SspI AAT'ATT
Cuts at: 5 52 5020 5
Size: 47 4968 2081
Fragments arranged by size:
4968 2081 47
StuI AGG'CCT
Cuts at: 1517 3446 1517
Size: 1929 5167
StyI C'CwwG_G
Cuts at: 513 1065 2022 2142 2365 2506 3132 3567 4535
Size: 552 957 120 223 141 626 435 968
Cuts at: 4535 513
Size: 3074
Fragments arranged by size:
3074 968 957 626 552 435 223 141
120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2912 4119 4360 5123 5140 5293 5478
Size: 1255 1332 1207 241 763 17 153 185
Cuts at: 5478 5923 325
Size: 445 1498
Fragments arranged by size:
1498 1332 1255 1207 763 445 241 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2091 3387 3747
Size: 274 80 33 51 1443 114 1296 360
Cuts at: 3747 5342 7054 96
Size: 1595 1712 138
Fragments arranged by size:
1712 1595 1443 1296 360 274 138 114
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2861 2893 4218 4250 7056 7088 2861
Size: 32 1325 32 2806 32 2869
Fragments arranged by size:
2869 2806 1325 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2269 1316
Size: 953 6143
XbaI T'CTAG_A
Cuts at: 4432 4432
Size: 7096
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3133 3328 3541 3133
Size: 195 213 6688
Fragments arranged by size:
6688 213 195
XhoI C'TCGA_G
Cuts at: 1943 2318 2789 1943
Size: 375 471 6250
Fragments arranged by size:
6250 471 375
XmaI C'CCGG_G
Cuts at: 1870 3363 4086 4134 4344 4421 1870
Size: 1493 723 48 210 77 4545
Fragments arranged by size:
4545 1493 723 210 77 48
XmnI GAAnn'nnTTC
Cuts at: 5225 5225
Size: 7096
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4080 4904 276
Size: 53 83 186 936 2546 824 2468
Fragments arranged by size:
2546 2468 936 824 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SanDI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PsrI SacII
SapI SfiI SwaI