Restriction map for
pFN21ASDA1229
(Circular) (Six-base) MAPSORT of: pFN21ASDA1229.seq Check: 6042 from: 1 to: 6637
pFN21ASDA1229 6637 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4447 278
Size: 53 83 186 936 2911 2468
Fragments arranged by size:
2911 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3980 1937
Size: 2043 4594
Acc65I G'GTAC_C
Cuts at: 3958 3958
Size: 6637
AclI AA'CG_TT
Cuts at: 1563 4764 5137 1563
Size: 3201 373 3063
Fragments arranged by size:
3201 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2159 2990 3428 3515 3626 3816 4700 6084
Size: 527 831 438 87 111 190 884 1384
Cuts at: 6084 6352 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 884 831 527 438 268 190
111 87
AflII C'TTAA_G
Cuts at: 820 1017 2183 2462 3695 3710 820
Size: 197 1166 279 1233 15 3747
Fragments arranged by size:
3747 1233 1166 279 197 15
AflIII A'CryG_T
Cuts at: 1203 5536 6513 1203
Size: 4333 977 1327
Fragments arranged by size:
4333 1327 977
AgeI A'CCGG_T
Cuts at: 5468 5468
Size: 6637
AhdI GACnn_n'nnGTC
Cuts at: 5366 5366
Size: 6637
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6637
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6637
AlwNI CAG_nnn'CTG
Cuts at: 4380 5952 4380
Size: 1572 5065
ApaLI G'TGCA_C
Cuts at: 4694 5850 4694
Size: 1156 5481
ApoI r'AATT_y
Cuts at: 1493 2287 2598 2995 3009 3325 3946 4172 4324
Size: 794 311 397 14 316 621 226 152
Cuts at: 4324 6433 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 794 621 397 316 311 226
152 14
AseI AT'TA_AT
Cuts at: 160 1032 2256 2318 2952 5191 160
Size: 872 1224 62 634 2239 1606
Fragments arranged by size:
2239 1606 1224 872 634 62
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6637
AvaI C'yCGr_G
Cuts at: 1870 1943 3962 1870
Size: 73 2019 4545
Fragments arranged by size:
4545 2019 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3269 3302 3269
Size: 33 6604
BamHI G'GATC_C
Cuts at: 3967 4334 3967
Size: 367 6270
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3958 5413 618
Size: 325 217 627 2171 1455 1842
Fragments arranged by size:
2171 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3956 1391
Size: 2565 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2988 3787 928
Size: 971 1089 799 3778
Fragments arranged by size:
3778 1089 971 799
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4828 4862 1903
Size: 34 2891 34 3678
Fragments arranged by size:
3678 2891 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4529 5739 4529
Size: 1210 5427
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6637
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5248 136
Size: 107 122 71 631 605 3576 1525
Fragments arranged by size:
3576 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2965 3850 6632 2965
Size: 885 2782 2970
Fragments arranged by size:
2970 2782 885
BlpI GC'TnA_GC
Cuts at: 4054 4054
Size: 6637
Bme1580I G_kGCm'C
Cuts at: 1790 4698 5854 1790
Size: 2908 1156 2573
Fragments arranged by size:
2908 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6637
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4368 5326 442
Size: 416 53 83 399 24 2951 958 1753
Fragments arranged by size:
2951 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6637
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5297 1239
Size: 4058 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3080 3683 4762 5627 5925 3080
Size: 603 1079 865 298 3792
Fragments arranged by size:
3792 1079 865 603 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5300 882
Size: 617 3801 2219
Fragments arranged by size:
3801 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3934 4428 493
Size: 711 2730 494 2702
Fragments arranged by size:
2730 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3549 6631 3549
Size: 3082 3555
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4444 4826 5461 275
Size: 53 83 186 936 2911 382 635 1451
Fragments arranged by size:
2911 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5070 5468 5742 5889 6300 5070
Size: 398 274 147 411 5407
Fragments arranged by size:
5407 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6412
Fragments arranged by size:
6412 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6637
BseYI C'CCAG_C
Cuts at: 5840 6336 5840
Size: 496 6141
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3522 1163
Size: 573 1786 4278
Fragments arranged by size:
4278 1786 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4087 4848 5876 1540
Size: 275 186 2086 761 1028 2301
Fragments arranged by size:
2301 2086 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4698 4783 5854 4698
Size: 85 1071 5481
Fragments arranged by size:
5481 1071 85
BsmI GAATG_Cn'
Cuts at: 4148 4241 4148
Size: 93 6544
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3956 4698 4783 5854 1391
Size: 399 2166 742 85 1071 2174
Fragments arranged by size:
2174 2166 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6300 6300
Size: 6637
BspHI T'CATG_A
Cuts at: 4526 6256 4526
Size: 1730 4907
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2685 3992 844
Size: 1841 1307 3489
Fragments arranged by size:
3489 1841 1307
BsrBI CCG'CTC
Cuts at: 1394 1823 4524 1394
Size: 429 2701 3507
Fragments arranged by size:
3507 2701 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5132 5306 65
Size: 1183 3884 174 1396
Fragments arranged by size:
3884 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5281 5468 1700
Size: 110 3471 187 2869
Fragments arranged by size:
3471 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6637
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6637
BssSI C'ACGA_G
Cuts at: 3461 4697 5709 3461
Size: 1236 1012 4389
Fragments arranged by size:
4389 1236 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6637
BstBI TT'CG_AA
Cuts at: 2007 5441 2007
Size: 3434 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6637
BstXI CCAn_nnnn'nTGG
Cuts at: 3545 3757 3545
Size: 212 6425
BstYI r'GATC_y
Cuts at: 1972 2592 2965 3850 3967 4334 4719 4736 6188
Size: 620 373 885 117 367 385 17 1452
Cuts at: 6188 6274 6286 6632 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 885 620 385 373 367 346
117 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2105 2986 513
Size: 552 957 83 881 4164
Fragments arranged by size:
4164 957 881 552 83
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5898
Fragments arranged by size:
5898 683 56
BtsI GCAGTG_nn'
Cuts at: 3488 4153 4947 4967 3488
Size: 665 794 20 5158
Fragments arranged by size:
5158 794 665 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6637
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6602
DraI TTT'AAA
Cuts at: 2748 3718 3941 2748
Size: 970 223 5444
Fragments arranged by size:
5444 970 223
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5644 809
Size: 507 4328 1802
Fragments arranged by size:
4328 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3747 4084 4973
Size: 54 1425 50 275 18 1917 337 889
Cuts at: 4973 6602 8
Size: 1629 43
Fragments arranged by size:
1917 1629 1425 889 337 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4084 1537
Size: 275 2272 4090
Fragments arranged by size:
4090 2272 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3576 3876 4567 1353
Size: 2223 300 691 3423
Fragments arranged by size:
3423 2223 691 300
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3461 5224 5598 5744 387
Size: 1064 138 1872 1763 374 146 1280
Fragments arranged by size:
1872 1763 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2159 2990 3428 3515 3626 3816 4700
Size: 393 527 831 438 87 111 190 884
Cuts at: 4700 5297 6084 6352 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 884 831 787 597 527 438 393
268 190 111 87
EcoNI CCTnn'n_nnAGG
Cuts at: 2141 2141
Size: 6637
EcoRI G'AATT_C
Cuts at: 3946 4324 3946
Size: 378 6259
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2198 3029 3710 2198
Size: 831 681 5125
Fragments arranged by size:
5125 831 681
FspI TGC'GCA
Cuts at: 5143 5143
Size: 6637
HaeII r_GCGC'y
Cuts at: 1678 5784 1678
Size: 4106 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2393 2425 2577 2609 3385
Size: 32 135 32 821 32 152 32 776
Cuts at: 3385 3417 3797 3829 5278 5310 5352 5384 1373
Size: 32 380 32 1449 32 42 32 2626
Fragments arranged by size:
2626 1449 821 776 380 152 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3981 4227 669
Size: 988 281 2043 246 3079
Fragments arranged by size:
3079 2043 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2807 3077 3569 748
Size: 2059 270 492 3816
Fragments arranged by size:
3816 2059 492 270
HpaI GTT'AAC
Cuts at: 4227 4227
Size: 6637
KpnI G_GTAC'C
Cuts at: 3962 3962
Size: 6637
MfeI C'AATT_G
Cuts at: 3030 4236 3030
Size: 1206 5431
MluI A'CGCG_T
Cuts at: 6513 6513
Size: 6637
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5751 5935 1305
Size: 4446 184 2007
Fragments arranged by size:
4446 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3749 10
Size: 54 1425 343 1917 2898
Fragments arranged by size:
2898 1917 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3042 4595 4954 5113 518
Size: 648 1876 1553 359 159 2042
Fragments arranged by size:
2042 1876 1553 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2133 3299 3604 4053 4730 5878 6123 1606
Size: 527 1166 305 449 677 1148 245 2120
Fragments arranged by size:
2120 1166 1148 677 527 449 305 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 2986 513
Size: 552 957 964 4164
Fragments arranged by size:
4164 964 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6637
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6637
NotI GC'GGCC_GC
Cuts at: 4084 4084
Size: 6637
NspI r_CATG'y
Cuts at: 1132 1229 2419 3995 5540 1132
Size: 97 1190 1576 1545 2229
Fragments arranged by size:
2229 1576 1545 1190 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6637
PciI A'CATG_T
Cuts at: 5536 5536
Size: 6637
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2871 1108
Size: 1763 4874
PmeI GTTT'AAAC
Cuts at: 3941 3941
Size: 6637
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4706 4738 6219 6251 4706
Size: 32 1481 32 5092
Fragments arranged by size:
5092 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6637
PsiI TTA'TAA
Cuts at: 4207 4207
Size: 6637
PstI C_TGCA'G
Cuts at: 830 3483 3733 3989 830
Size: 2653 250 256 3478
Fragments arranged by size:
3478 2653 256 250
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6637
PvuII CAG'CTG
Cuts at: 2133 3299 3604 2133
Size: 1166 305 5166
Fragments arranged by size:
5166 1166 305
SalI G'TCGA_C
Cuts at: 1936 3979 1936
Size: 2043 4594
SapI GCTCTTCn'nnn_
Cuts at: 3876 3876
Size: 6637
SbfI CC_TGCA'GG
Cuts at: 3989 3989
Size: 6637
ScaI AGT'ACT
Cuts at: 4885 6597 4885
Size: 1712 4925
SfcI C'TryA_G
Cuts at: 826 1045 2211 2588 2706 2739 3479 3729 3985
Size: 219 1166 377 118 33 740 250 256
Cuts at: 3985 5120 5801 5992 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1166 1135 740 681 377 256 250
219 191 118 33
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6637
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6637
SmaI CCC'GGG
Cuts at: 1872 3964 1872
Size: 2092 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2183 2462 3059 3662 3695 3710
Size: 197 926 240 279 597 603 33 15
Cuts at: 3710 4741 5642 5904 820
Size: 1031 901 262 1553
Fragments arranged by size:
1553 1031 926 901 603 597 279 262
240 197 33 15
SnaBI TAC'GTA
Cuts at: 493 3934 4428 493
Size: 3441 494 2702
Fragments arranged by size:
3441 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6637
SphI G_CATG'C
Cuts at: 1132 3995 1132
Size: 2863 3774
SspI AAT'ATT
Cuts at: 5 52 2316 3846 4561 5
Size: 47 2264 1530 715 2081
Fragments arranged by size:
2264 2081 1530 715 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6637
StyI C'CwwG_G
Cuts at: 513 1065 2022 2986 4076 513
Size: 552 957 964 1090 3074
Fragments arranged by size:
3074 1090 964 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4664 4681 4834 5019 5464 325
Size: 1255 3084 17 153 185 445 1498
Fragments arranged by size:
3084 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2575 2920 4883
Size: 274 80 33 51 1443 598 345 1963
Cuts at: 4883 6595 96
Size: 1712 138
Fragments arranged by size:
1963 1712 1443 598 345 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6597 6629 6597
Size: 32 6605
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6637
XbaI T'CTAG_A
Cuts at: 3818 3973 3818
Size: 155 6482
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6637
XmaI C'CCGG_G
Cuts at: 1870 3962 1870
Size: 2092 4545
XmnI GAAnn'nnTTC
Cuts at: 2050 2367 2995 3009 3618 4766 2050
Size: 317 628 14 609 1148 3921
Fragments arranged by size:
3921 1148 628 609 317 14
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4445 276
Size: 53 83 186 936 2911 2468
Fragments arranged by size:
2911 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI