Restriction map for
pFN21ASDA1227
(Circular) (Six-base) MAPSORT of: pFN21ASDA1227.seq Check: 5222 from: 1 to: 7927
pFN21ASDA1227 7927 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5737 278
Size: 53 83 186 936 4201 2468
Fragments arranged by size:
4201 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5270 1937
Size: 3333 4594
Acc65I G'GTAC_C
Cuts at: 5248 5248
Size: 7927
AclI AA'CG_TT
Cuts at: 1563 6054 6427 1563
Size: 4491 373 3063
Fragments arranged by size:
4491 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4328 4557 4701 4896 5990 7374 7642 1632
Size: 2696 229 144 195 1094 1384 268 1917
Fragments arranged by size:
2696 1917 1384 1094 268 229 195 144
AflII C'TTAA_G
Cuts at: 820 1017 3677 820
Size: 197 2660 5070
Fragments arranged by size:
5070 2660 197
AflIII A'CryG_T
Cuts at: 1203 2405 2590 3720 4557 4842 4899 6826 7803
Size: 1202 185 1130 837 285 57 1927 977
Cuts at: 7803 1203
Size: 1327
Fragments arranged by size:
1927 1327 1202 1130 977 837 285 185
57
AgeI A'CCGG_T
Cuts at: 6758 6758
Size: 7927
AhdI GACnn_n'nnGTC
Cuts at: 3801 6656 3801
Size: 2855 5072
AleI CACnn'nnGTG
Cuts at: 1166 4473 1166
Size: 3307 4620
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7927
AlwNI CAG_nnn'CTG
Cuts at: 3389 3602 4392 5017 5670 7242 3389
Size: 213 790 625 653 1572 4074
Fragments arranged by size:
4074 1572 790 653 625 213
ApaLI G'TGCA_C
Cuts at: 5984 7140 5984
Size: 1156 6771
ApoI r'AATT_y
Cuts at: 1493 3620 3656 3872 5236 5462 5614 7723 1493
Size: 2127 36 216 1364 226 152 2109 1697
Fragments arranged by size:
2127 2109 1697 1364 226 216 152 36
AseI AT'TA_AT
Cuts at: 160 1032 6481 160
Size: 872 5449 1606
Fragments arranged by size:
5449 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7927
AvaI C'yCGr_G
Cuts at: 1870 1943 4014 4301 4355 4694 5252 1870
Size: 73 2071 287 54 339 558 4545
Fragments arranged by size:
4545 2071 558 339 287 73 54
BamHI G'GATC_C
Cuts at: 5257 5624 5257
Size: 367 7560
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4398 4432 4690 5248 6703
Size: 325 217 627 2611 34 258 558 1455
Cuts at: 6703 618
Size: 1842
Fragments arranged by size:
2611 1842 1455 627 558 325 258 217
34
BanII G_rGCy'C
Cuts at: 1391 2152 3321 4518 5246 1391
Size: 761 1169 1197 728 4072
Fragments arranged by size:
4072 1197 1169 761 728
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3695 4604 4626 928
Size: 971 1796 909 22 4229
Fragments arranged by size:
4229 1796 971 909 22
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6118 6152 1903
Size: 34 4181 34 3678
Fragments arranged by size:
4181 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5819 7029 5819
Size: 1210 6717
BclI T'GATC_A
Cuts at: 1548 3927 1548
Size: 2379 5548
BfrBI ATG'CAT
Cuts at: 4096 4096
Size: 7927
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5037 6538 136
Size: 107 122 71 631 605 3365 1501 1525
Fragments arranged by size:
3365 1525 1501 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3681 4010 7922 3681
Size: 329 3912 3686
Fragments arranged by size:
3912 3686 329
BlpI GC'TnA_GC
Cuts at: 4209 5344 4209
Size: 1135 6792
Bme1580I G_kGCm'C
Cuts at: 1790 2080 4949 5085 5988 7144 1790
Size: 290 2869 136 903 1156 2573
Fragments arranged by size:
2869 2573 1156 903 290 136
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7927
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5658 6616 442
Size: 416 53 83 399 24 4241 958 1753
Fragments arranged by size:
4241 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7927
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3580 3580
Size: 7927
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4814 4900 5039 5043 6587 1239
Size: 3575 86 139 4 1544 2579
Fragments arranged by size:
3575 2579 1544 139 86 4
Bpu10I CC'TnA_GC
Cuts at: 4512 4512
Size: 7927
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2373 2702 3204 3550 4027 6052 6917 7215 2373
Size: 329 502 346 477 2025 865 298 3085
Fragments arranged by size:
3085 2025 865 502 477 346 329 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2127 2836 3408 3537 3637 6590 882
Size: 617 628 709 572 129 100 2953 2219
Fragments arranged by size:
2953 2219 709 628 617 572 129 100
BsaAI yAC'GTr
Cuts at: 493 1204 4843 5224 5718 493
Size: 711 3639 381 494 2702
Fragments arranged by size:
3639 2702 711 494 381
BsaBI GATnn'nnATC
Cuts at: 7921 7921
Size: 7927
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5734 6116 6751 275
Size: 53 83 186 936 4201 382 635 1451
Fragments arranged by size:
4201 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6360 6758 7032 7179 7590 6360
Size: 398 274 147 411 6697
Fragments arranged by size:
6697 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4799 4829 4948 4978 1178
Size: 30 165 30 3396 30 119 30 4127
Fragments arranged by size:
4127 3396 165 119 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2102 3029 4143 4676 4688 1186
Size: 916 927 1114 533 12 4425
Fragments arranged by size:
4425 1114 927 916 533 12
BseYI C'CCAG_C
Cuts at: 3320 3463 4506 7130 7626 3320
Size: 143 1043 2624 496 3621
Fragments arranged by size:
3621 2624 1043 496 143
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2037 1163
Size: 573 301 7053
Fragments arranged by size:
7053 573 301
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4266 5377 6138 7166 1540
Size: 275 186 2265 1111 761 1028 2301
Fragments arranged by size:
2301 2265 1111 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2152 3528 4820 5988 6073 7144 2152
Size: 1376 1292 1168 85 1071 2935
Fragments arranged by size:
2935 1376 1292 1168 1071 85
BsmI GAATG_Cn'
Cuts at: 2686 5438 5531 2686
Size: 2752 93 5082
Fragments arranged by size:
5082 2752 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2099 2099
Size: 7927
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2080 2152 3321 3528 4518 4820 4949
Size: 399 290 72 1169 207 990 302 129
Cuts at: 4949 5085 5246 5988 6073 7144 1391
Size: 136 161 742 85 1071 2174
Fragments arranged by size:
2174 1169 1071 990 742 399 302 290
207 161 136 129 85 72
BspEI T'CCGG_A
Cuts at: 7590 7590
Size: 7927
BspHI T'CATG_A
Cuts at: 5816 7546 5816
Size: 1730 6197
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3689 4358 5282 844
Size: 2845 669 924 3489
Fragments arranged by size:
3489 2845 924 669
BsrBI CCG'CTC
Cuts at: 1394 1823 4246 5814 1394
Size: 429 2423 1568 3507
Fragments arranged by size:
3507 2423 1568 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2559 3670 6422 6596 65
Size: 1183 1311 1111 2752 174 1396
Fragments arranged by size:
2752 1396 1311 1183 1111 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6571 6758 1700
Size: 110 4761 187 2869
Fragments arranged by size:
4761 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7927
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7927
BssSI C'ACGA_G
Cuts at: 4241 4319 5987 6999 4241
Size: 78 1668 1012 5169
Fragments arranged by size:
5169 1668 1012 78
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3672 794
Size: 2878 5049
BstBI TT'CG_AA
Cuts at: 2007 6731 2007
Size: 4724 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7927
BstYI r'GATC_y
Cuts at: 1972 2693 2984 3681 4010 5007 5257 5624 6009
Size: 721 291 697 329 997 250 367 385
Cuts at: 6009 6026 7478 7564 7576 7922 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 997 721 697 385 367 346
329 291 250 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3242 4286 3242
Size: 1044 6883
BtgI C'CryG_G
Cuts at: 513 1065 2022 2846 513
Size: 552 957 824 5594
Fragments arranged by size:
5594 957 824 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3221 487
Size: 683 56 1995 5193
Fragments arranged by size:
5193 1995 683 56
BtsI GCAGTG_nn'
Cuts at: 2745 4997 5443 6237 6257 2745
Size: 2252 446 794 20 4415
Fragments arranged by size:
4415 2252 794 446 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7927
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2841 2876 529
Size: 35 2277 35 5580
Fragments arranged by size:
5580 2277 35 35
DraI TTT'AAA
Cuts at: 2274 3180 3661 5140 5231 2274
Size: 906 481 1479 91 4970
Fragments arranged by size:
4970 1479 906 481 91
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4881 6934 809
Size: 507 3565 2053 1802
Fragments arranged by size:
3565 2053 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2645 2849 4263
Size: 54 1425 50 275 18 815 204 1414
Cuts at: 4263 5374 6263 7892 8
Size: 1111 889 1629 43
Fragments arranged by size:
1629 1425 1414 1111 889 815 275 204
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4263 5374 1537
Size: 275 2451 1111 4090
Fragments arranged by size:
4090 2451 1111 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2303 2834 4222 4680 5857 1353
Size: 950 531 1388 458 1177 3423
Fragments arranged by size:
3423 1388 1177 950 531 458
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6514 6888 7034 387
Size: 1064 138 4925 374 146 1280
Fragments arranged by size:
4925 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2150 2150
Size: 7927
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4328 4557 4701 4814 4896 4900 5039
Size: 393 2696 229 144 113 82 4 139
Cuts at: 5039 5043 5990 6587 7374 7642 1239
Size: 4 947 597 787 268 1524
Fragments arranged by size:
2696 1524 947 787 597 393 268 229
144 139 113 82 4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2084 2084
Size: 7927
EcoO109I rG'GnC_Cy
Cuts at: 3246 3922 4497 4724 3246
Size: 676 575 227 6449
Fragments arranged by size:
6449 676 575 227
EcoRI G'AATT_C
Cuts at: 5236 5614 5236
Size: 378 7549
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2556 2980 4021 2556
Size: 424 1041 6462
Fragments arranged by size:
6462 1041 424
FspI TGC'GCA
Cuts at: 3305 4367 6433 3305
Size: 1062 2066 4799
Fragments arranged by size:
4799 2066 1062
FspAI rTGC'GCAy
Cuts at: 3305 4367 3305
Size: 1062 6865
HaeII r_GCGC'y
Cuts at: 1678 7074 1678
Size: 5396 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2875 2907 3086 3118 3515
Size: 32 135 32 1303 32 179 32 397
Cuts at: 3515 3547 3787 3819 4999 5031 6568 6600 6642
Size: 32 240 32 1180 32 1537 32 42
Cuts at: 6642 6674 1373
Size: 32 2626
Fragments arranged by size:
2626 1537 1303 1180 397 240 179 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3267 3718 4585 5271 5517 669
Size: 988 281 1329 451 867 686 246 3079
Fragments arranged by size:
3079 1329 988 867 686 451 281 246
HindIII A'AGCT_T
Cuts at: 748 4854 748
Size: 4106 3821
HpaI GTT'AAC
Cuts at: 3718 4585 5517 3718
Size: 867 932 6128
Fragments arranged by size:
6128 932 867
KpnI G_GTAC'C
Cuts at: 5252 5252
Size: 7927
MfeI C'AATT_G
Cuts at: 5526 5526
Size: 7927
MluI A'CGCG_T
Cuts at: 7803 7803
Size: 7927
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2110 2426 3315 3612 7041 7225 1305
Size: 805 316 889 297 3429 184 2007
Fragments arranged by size:
3429 2007 889 805 316 297 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6105
Fragments arranged by size:
6105 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3711 4473 5885 6244 6403 518
Size: 648 2545 762 1412 359 159 2042
Fragments arranged by size:
2545 2042 1412 762 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2107 3331 3389 4792 5343 6020 7168 7413
Size: 501 1224 58 1403 551 677 1148 245
Cuts at: 7413 1606
Size: 2120
Fragments arranged by size:
2120 1403 1224 1148 677 551 501 245
58
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 6418
Fragments arranged by size:
6418 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7927
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7927
NotI GC'GGCC_GC
Cuts at: 5374 5374
Size: 7927
NsiI A_TGCA'T
Cuts at: 4098 4098
Size: 7927
NspI r_CATG'y
Cuts at: 1132 1229 2594 3724 3857 4151 4366 4903 5285
Size: 97 1365 1130 133 294 215 537 382
Cuts at: 5285 6830 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 1365 1130 537 382 294 215
133 97
PasI CC'CwG_GG
Cuts at: 1348 3109 1348
Size: 1761 6166
PciI A'CATG_T
Cuts at: 2590 3720 4899 6826 2590
Size: 1130 1179 1927 3691
Fragments arranged by size:
3691 1927 1179 1130
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2981 4746 5017 1108
Size: 1873 1765 271 4018
Fragments arranged by size:
4018 1873 1765 271
PfoI T'CCnGG_A
Cuts at: 4099 4099
Size: 7927
PmeI GTTT'AAAC
Cuts at: 5231 5231
Size: 7927
PmlI CAC'GTG
Cuts at: 4843 4843
Size: 7927
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5996 6028 7509 7541 5996
Size: 32 1481 32 6382
Fragments arranged by size:
6382 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3246 4724 3246
Size: 1478 6449
PshAI GACnn'nnGTC
Cuts at: 1613 3759 4626 1613
Size: 2146 867 4914
Fragments arranged by size:
4914 2146 867
PsiI TTA'TAA
Cuts at: 5497 5497
Size: 7927
PspXI vC'TCGA_Gb
Cuts at: 4355 4355
Size: 7927
PstI C_TGCA'G
Cuts at: 830 4283 5279 830
Size: 3453 996 3478
Fragments arranged by size:
3478 3453 996
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7927
PvuII CAG'CTG
Cuts at: 2107 3389 4792 2107
Size: 1282 1403 5242
Fragments arranged by size:
5242 1403 1282
RsrII CG'GwC_CG
Cuts at: 3487 3487
Size: 7927
SacI G_AGCT'C
Cuts at: 2152 2152
Size: 7927
SalI G'TCGA_C
Cuts at: 1936 5269 1936
Size: 3333 4594
SapI GCTCTTCn'nnn_
Cuts at: 2303 2303
Size: 7927
SbfI CC_TGCA'GG
Cuts at: 5279 5279
Size: 7927
ScaI AGT'ACT
Cuts at: 6175 7887 6175
Size: 1712 6215
SexAI A'CCwGG_T
Cuts at: 2788 4401 2788
Size: 1613 6314
SfcI C'TryA_G
Cuts at: 826 1045 2341 4279 5275 6410 7091 7282 826
Size: 219 1296 1938 996 1135 681 191 1471
Fragments arranged by size:
1938 1471 1296 1135 996 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7927
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7927
SmaI CCC'GGG
Cuts at: 1872 5254 1872
Size: 3382 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2388 2681 3219 3565 3677 4006
Size: 197 926 445 293 538 346 112 329
Cuts at: 4006 4014 4355 6031 6932 7194 820
Size: 8 341 1676 901 262 1553
Fragments arranged by size:
1676 1553 926 901 538 445 346 341
329 293 262 197 112 8
SnaBI TAC'GTA
Cuts at: 493 5224 5718 493
Size: 4731 494 2702
Fragments arranged by size:
4731 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7927
SphI G_CATG'C
Cuts at: 1132 4151 4366 5285 1132
Size: 3019 215 919 3774
Fragments arranged by size:
3774 3019 919 215
SspI AAT'ATT
Cuts at: 5 52 5851 5
Size: 47 5799 2081
Fragments arranged by size:
5799 2081 47
StuI AGG'CCT
Cuts at: 1517 3217 5040 1517
Size: 1700 1823 4404
Fragments arranged by size:
4404 1823 1700
StyI C'CwwG_G
Cuts at: 513 1065 2022 4114 5366 513
Size: 552 957 2092 1252 3074
Fragments arranged by size:
3074 2092 1252 957 552
SwaI ATTT'AAAT
Cuts at: 3661 3661
Size: 7927
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3394 3504 4450 5954 5971 6124 6309
Size: 1255 1814 110 946 1504 17 153 185
Cuts at: 6309 6754 325
Size: 445 1498
Fragments arranged by size:
1814 1504 1498 1255 946 445 185 153
110 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2946 4037 6173
Size: 274 80 33 51 1443 969 1091 2136
Cuts at: 6173 7885 96
Size: 1712 138
Fragments arranged by size:
2136 1712 1443 1091 969 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4799 4831 4948 4980 7887 7919 4799
Size: 32 117 32 2907 32 4807
Fragments arranged by size:
4807 2907 117 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7927
XbaI T'CTAG_A
Cuts at: 2824 5263 2824
Size: 2439 5488
XhoI C'TCGA_G
Cuts at: 1943 4014 4355 1943
Size: 2071 341 5515
Fragments arranged by size:
5515 2071 341
XmaI C'CCGG_G
Cuts at: 1870 5252 1870
Size: 3382 4545
XmnI GAAnn'nnTTC
Cuts at: 2184 2613 4772 6056 2184
Size: 429 2159 1284 4055
Fragments arranged by size:
4055 2159 1284 429
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5735 276
Size: 53 83 186 936 4201 2468
Fragments arranged by size:
4201 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BsiWI BstXI BstZ17I DraIII EcoRV FseI KasI
NaeI NarI NgoMIV NruI PacI PspOMI PsrI SacII
SanDI SfiI SfoI SrfI XcmI