Restriction map for
pFN21ASDA1225
(Circular) (Six-base) MAPSORT of: pFN21ASDA1225.seq Check: 3114 from: 1 to: 8842
pFN21ASDA1225 8842 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6652 278
Size: 53 83 186 936 5116 2468
Fragments arranged by size:
5116 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2071 6185 1937
Size: 134 4114 4594
Fragments arranged by size:
4594 4114 134
Acc65I G'GTAC_C
Cuts at: 2056 6163 2056
Size: 4107 4735
AclI AA'CG_TT
Cuts at: 1563 2270 6969 7342 1563
Size: 707 4699 373 3063
Fragments arranged by size:
4699 3063 707 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2643 2801 3735 3837 4602 5343 6003 6905
Size: 1011 158 934 102 765 741 660 902
Cuts at: 6905 8289 8557 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1011 934 902 765 741 660
268 158 102
AflII C'TTAA_G
Cuts at: 820 1017 2992 3494 820
Size: 197 1975 502 6168
Fragments arranged by size:
6168 1975 502 197
AflIII A'CryG_T
Cuts at: 1203 2774 3297 7741 8718 1203
Size: 1571 523 4444 977 1327
Fragments arranged by size:
4444 1571 1327 977 523
AgeI A'CCGG_T
Cuts at: 7673 7673
Size: 8842
AhdI GACnn_n'nnGTC
Cuts at: 7571 7571
Size: 8842
AleI CACnn'nnGTG
Cuts at: 1166 2615 1166
Size: 1449 7393
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8842
AlwNI CAG_nnn'CTG
Cuts at: 3749 5276 6585 8157 3749
Size: 1527 1309 1572 4434
Fragments arranged by size:
4434 1572 1527 1309
ApaLI G'TGCA_C
Cuts at: 6899 8055 6899
Size: 1156 7686
ApoI r'AATT_y
Cuts at: 1493 2488 2606 2766 3166 3457 4123 5107 6151
Size: 995 118 160 400 291 666 984 1044
Cuts at: 6151 6377 6529 8638 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1044 995 984 666 400 291
226 160 152 118
AseI AT'TA_AT
Cuts at: 160 1032 2298 3141 3869 4095 4416 5469 7396
Size: 872 1266 843 728 226 321 1053 1927
Cuts at: 7396 160
Size: 1606
Fragments arranged by size:
1927 1606 1266 1053 872 843 728 321
226
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8842
AvaI C'yCGr_G
Cuts at: 1870 1943 6167 1870
Size: 73 4224 4545
Fragments arranged by size:
4545 4224 73
BamHI G'GATC_C
Cuts at: 5884 6172 6539 5884
Size: 288 367 8187
Fragments arranged by size:
8187 367 288
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2056 3439 6163 7618 618
Size: 325 217 627 269 1383 2724 1455 1842
Fragments arranged by size:
2724 1842 1455 1383 627 325 269 217
BanII G_rGCy'C
Cuts at: 1391 3397 5314 6161 1391
Size: 2006 1917 847 4072
Fragments arranged by size:
4072 2006 1917 847
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3426 3564 3629 928
Size: 971 1527 138 65 6141
Fragments arranged by size:
6141 1527 971 138 65
BbvCI CC'TCA_GC
Cuts at: 4698 5043 4698
Size: 345 8497
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7033 7067 1903
Size: 34 5096 34 3678
Fragments arranged by size:
5096 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4470 6734 7944 4470
Size: 2264 1210 5368
Fragments arranged by size:
5368 2264 1210
BclI T'GATC_A
Cuts at: 1548 3010 4783 1548
Size: 1462 1773 5607
Fragments arranged by size:
5607 1773 1462
BfrBI ATG'CAT
Cuts at: 5438 5438
Size: 8842
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5692 7453 136
Size: 107 122 71 631 605 4020 1761 1525
Fragments arranged by size:
4020 1761 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4585 8837 4585
Size: 4252 4590
BlpI GC'TnA_GC
Cuts at: 6259 6259
Size: 8842
Bme1580I G_kGCm'C
Cuts at: 1790 6903 8059 1790
Size: 5113 1156 2573
Fragments arranged by size:
5113 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8842
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2440 4507 6573
Size: 416 53 83 399 24 1023 2067 2066
Cuts at: 6573 7531 442
Size: 958 1753
Fragments arranged by size:
2067 2066 1753 1023 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8842
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5371 5371
Size: 8842
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4053 5565 5629 7502 1239
Size: 2814 1512 64 1873 2579
Fragments arranged by size:
2814 2579 1873 1512 64
Bpu10I CC'TnA_GC
Cuts at: 4698 5043 4698
Size: 345 8497
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2492 2651 3113 3381 6967 7832 8130 2492
Size: 159 462 268 3586 865 298 3204
Fragments arranged by size:
3586 3204 865 462 298 268 159
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2081 3263 3542 5389 7505 882
Size: 617 582 1182 279 1847 2116 2219
Fragments arranged by size:
2219 2116 1847 1182 617 582 279
BsaAI yAC'GTr
Cuts at: 493 1204 6139 6633 493
Size: 711 4935 494 2702
Fragments arranged by size:
4935 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 2187 4866 8836 2187
Size: 2679 3970 2193
Fragments arranged by size:
3970 2679 2193
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6649 7031 7666 275
Size: 53 83 186 936 5116 382 635 1451
Fragments arranged by size:
5116 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 7275 7673 7947 8094 8505 7275
Size: 398 274 147 411 7612
Fragments arranged by size:
7612 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8617
Fragments arranged by size:
8617 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 5031 5361 1186
Size: 913 2932 330 4667
Fragments arranged by size:
4667 2932 913 330
BseYI C'CCAG_C
Cuts at: 2574 5170 8045 8541 2574
Size: 2596 2875 496 2875
Fragments arranged by size:
2875 2875 2596 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4908 5391 1163
Size: 573 3172 483 4614
Fragments arranged by size:
4614 3172 573 483
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5212 6292 7053 8081 1540
Size: 275 186 3211 1080 761 1028 2301
Fragments arranged by size:
3211 2301 1080 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4440 5069 5458 6903 6988 8059 4440
Size: 629 389 1445 85 1071 5223
Fragments arranged by size:
5223 1445 1071 629 389 85
BsmI GAATG_Cn'
Cuts at: 5563 6353 6446 5563
Size: 790 93 7959
Fragments arranged by size:
7959 790 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3397 4440 5069 5314 5458 6161 6903
Size: 399 1607 1043 629 245 144 703 742
Cuts at: 6903 6988 8059 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1607 1071 1043 742 703 629 399
245 144 85
BspEI T'CCGG_A
Cuts at: 8505 8505
Size: 8842
BspHI T'CATG_A
Cuts at: 4189 6731 8461 4189
Size: 2542 1730 4570
Fragments arranged by size:
4570 2542 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4786 6197 844
Size: 3942 1411 3489
Fragments arranged by size:
3942 3489 1411
BsrBI CCG'CTC
Cuts at: 1394 1823 6729 1394
Size: 429 4906 3507
Fragments arranged by size:
4906 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2113 2283 4790 7337 7511 65
Size: 1183 865 170 2507 2547 174 1396
Fragments arranged by size:
2547 2507 1396 1183 865 174 170
BsrFI r'CCGG_y
Cuts at: 1700 1810 7486 7673 1700
Size: 110 5676 187 2869
Fragments arranged by size:
5676 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3526 3694 96
Size: 3430 168 5244
Fragments arranged by size:
5244 3430 168
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8842
BssSI C'ACGA_G
Cuts at: 5601 6902 7914 5601
Size: 1301 1012 6529
Fragments arranged by size:
6529 1301 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2285 794
Size: 1491 7351
BstBI TT'CG_AA
Cuts at: 2007 7646 2007
Size: 5639 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8842
BstYI r'GATC_y
Cuts at: 1972 4585 4616 5575 5884 6172 6539 6924 6941
Size: 2613 31 959 309 288 367 385 17
Cuts at: 6941 8393 8479 8491 8837 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2613 1977 1452 959 385 367 346 309
288 86 31 17 12
Bsu36I CC'TnA_GG
Cuts at: 5697 5730 5697
Size: 33 8809
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 8290
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8103
Fragments arranged by size:
8103 683 56
BtsI GCAGTG_nn'
Cuts at: 4978 6078 6358 7152 7172 4978
Size: 1100 280 794 20 6648
Fragments arranged by size:
6648 1100 794 280 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8842
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8807
DraI TTT'AAA
Cuts at: 3462 4158 6146 3462
Size: 696 1988 6158
Fragments arranged by size:
6158 1988 696
DraIII CAC_nnn'GTG
Cuts at: 4995 4995
Size: 8842
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7849 809
Size: 507 6533 1802
Fragments arranged by size:
6533 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6289 7178 8807
Size: 54 1425 50 275 18 4459 889 1629
Cuts at: 8807 8
Size: 43
Fragments arranged by size:
4459 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 6289 1537
Size: 275 4477 4090
Fragments arranged by size:
4477 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2076 2193 3252 3540 3562 3831 4230 4875
Size: 723 117 1059 288 22 269 399 645
Cuts at: 4875 6772 1353
Size: 1897 3423
Fragments arranged by size:
3423 1897 1059 723 645 399 288 269
117 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3866 7429 7803 7949 387
Size: 1064 138 2277 3563 374 146 1280
Fragments arranged by size:
3563 2277 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2643 2801 3735 3837 4053 4602 5343
Size: 393 1011 158 934 102 216 549 741
Cuts at: 5343 5565 5629 6003 6905 7502 8289 8557 1239
Size: 222 64 374 902 597 787 268 1524
Fragments arranged by size:
1524 1011 934 902 787 741 597 549
393 374 268 222 216 158 102 64
EcoNI CCTnn'n_nnAGG
Cuts at: 5835 5835
Size: 8842
EcoO109I rG'GnC_Cy
Cuts at: 4901 4901
Size: 8842
EcoRI G'AATT_C
Cuts at: 2606 6151 6529 2606
Size: 3545 378 4919
Fragments arranged by size:
4919 3545 378
EcoRV GAT'ATC
Cuts at: 3470 3470
Size: 8842
FspI TGC'GCA
Cuts at: 7348 7348
Size: 8842
HaeII r_GCGC'y
Cuts at: 1678 7989 1678
Size: 6311 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3212 3244 3935 3967 4817
Size: 32 135 32 1640 32 691 32 850
Cuts at: 4817 4849 5282 5314 5321 5353 7483 7515 7557
Size: 32 433 32 7 32 2130 32 42
Cuts at: 7557 7589 1373
Size: 32 2626
Fragments arranged by size:
2626 2130 1640 850 691 433 135 42
32 32 32 32 32 32 32 32
32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3790 4267 4558 6186 6432 669
Size: 988 281 1852 477 291 1628 246 3079
Fragments arranged by size:
3079 1852 1628 988 477 291 281 246
HindIII A'AGCT_T
Cuts at: 748 2958 4402 748
Size: 2210 1444 5188
Fragments arranged by size:
5188 2210 1444
HpaI GTT'AAC
Cuts at: 3790 6432 3790
Size: 2642 6200
KpnI G_GTAC'C
Cuts at: 2060 6167 2060
Size: 4107 4735
MfeI C'AATT_G
Cuts at: 2376 3197 3991 6441 2376
Size: 821 794 2450 4777
Fragments arranged by size:
4777 2450 821 794
MluI A'CGCG_T
Cuts at: 8718 8718
Size: 8842
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3497 4594 7956 8140 1305
Size: 2192 1097 3362 184 2007
Fragments arranged by size:
3362 2192 2007 1097 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 7020
Fragments arranged by size:
7020 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2615 3409 3798 4542 5124 6800 7159
Size: 648 1449 794 389 744 582 1676 359
Cuts at: 7159 7318 518
Size: 159 2042
Fragments arranged by size:
2042 1676 1449 794 744 648 582 389
359 159
MspA1I CmG'CkG
Cuts at: 1606 2431 2578 3391 4283 5360 6258 6935 8083
Size: 825 147 813 892 1077 898 677 1148
Cuts at: 8083 8328 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1077 898 892 825 813 677
245 147
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 8290
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8842
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8842
NotI GC'GGCC_GC
Cuts at: 6289 6289
Size: 8842
NsiI A_TGCA'T
Cuts at: 5440 5440
Size: 8842
NspI r_CATG'y
Cuts at: 1132 1229 2778 3301 6200 7745 1132
Size: 97 1549 523 2899 1545 2229
Fragments arranged by size:
2899 2229 1549 1545 523 97
PasI CC'CwG_GG
Cuts at: 1348 3447 1348
Size: 2099 6743
PciI A'CATG_T
Cuts at: 2774 3297 7741 2774
Size: 523 4444 3875
Fragments arranged by size:
4444 3875 523
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3044 3280 5848 5894 1108
Size: 1936 236 2568 46 4056
Fragments arranged by size:
4056 2568 1936 236 46
PfoI T'CCnGG_A
Cuts at: 5581 5581
Size: 8842
PmeI GTTT'AAAC
Cuts at: 6146 6146
Size: 8842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4163 4195 6911 6943 8424 8456 4163
Size: 32 2716 32 1481 32 4549
Fragments arranged by size:
4549 2716 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8842
PsiI TTA'TAA
Cuts at: 4334 6050 6412 4334
Size: 1716 362 6764
Fragments arranged by size:
6764 1716 362
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2011 2043 2011
Size: 32 8810
PstI C_TGCA'G
Cuts at: 830 4981 5227 6009 6194 830
Size: 4151 246 782 185 3478
Fragments arranged by size:
4151 3478 782 246 185
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8842
PvuII CAG'CTG
Cuts at: 2431 2578 4283 2431
Size: 147 1705 6990
Fragments arranged by size:
6990 1705 147
SalI G'TCGA_C
Cuts at: 1936 6184 1936
Size: 4248 4594
SapI GCTCTTCn'nnn_
Cuts at: 2193 4230 4875 2193
Size: 2037 645 6160
Fragments arranged by size:
6160 2037 645
SbfI CC_TGCA'GG
Cuts at: 6194 6194
Size: 8842
ScaI AGT'ACT
Cuts at: 2628 3637 5622 7090 8802 2628
Size: 1009 1985 1468 1712 2668
Fragments arranged by size:
2668 1985 1712 1468 1009
SfcI C'TryA_G
Cuts at: 826 1045 2904 3191 3642 4977 5223 6005 6040
Size: 219 1859 287 451 1335 246 782 35
Cuts at: 6040 6190 7325 8006 8197 826
Size: 150 1135 681 191 1471
Fragments arranged by size:
1859 1471 1335 1135 782 681 451 287
246 219 191 150 35
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8842
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8842
SmaI CCC'GGG
Cuts at: 1872 6169 1872
Size: 4297 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2471 2630 2992 3092 3396 3494
Size: 197 926 528 159 362 100 304 98
Cuts at: 3494 6946 7847 8109 820
Size: 3452 901 262 1553
Fragments arranged by size:
3452 1553 926 901 528 362 304 262
197 159 100 98
SnaBI TAC'GTA
Cuts at: 493 6139 6633 493
Size: 5646 494 2702
Fragments arranged by size:
5646 2702 494
SpeI A'CTAG_T
Cuts at: 152 5522 152
Size: 5370 3472
SphI G_CATG'C
Cuts at: 1132 6200 1132
Size: 5068 3774
SspI AAT'ATT
Cuts at: 5 52 2517 2746 6055 6766 5
Size: 47 2465 229 3309 711 2081
Fragments arranged by size:
3309 2465 2081 711 229 47
StuI AGG'CCT
Cuts at: 1517 5695 1517
Size: 4178 4664
StyI C'CwwG_G
Cuts at: 513 1065 2163 3314 6281 513
Size: 552 1098 1151 2967 3074
Fragments arranged by size:
3074 2967 1151 1098 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3104 3605 5226 6869 6886 7039 7224
Size: 1255 1524 501 1621 1643 17 153 185
Cuts at: 7224 7669 325
Size: 445 1498
Fragments arranged by size:
1643 1621 1524 1498 1255 501 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2626 3526 3635
Size: 274 80 33 51 1443 649 900 109
Cuts at: 3635 3694 4709 5237 5620 7088 8800 96
Size: 59 1015 528 383 1468 1712 138
Fragments arranged by size:
1712 1468 1443 1015 900 649 528 383
274 138 109 80 59 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4608 4640 6057 6089 8802 8834 4608
Size: 32 1417 32 2713 32 4616
Fragments arranged by size:
4616 2713 1417 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8842
XbaI T'CTAG_A
Cuts at: 5756 6178 5756
Size: 422 8420
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8842
XmaI C'CCGG_G
Cuts at: 1870 6167 1870
Size: 4297 4545
XmnI GAAnn'nnTTC
Cuts at: 2130 2202 2784 6971 2130
Size: 72 582 4187 4001
Fragments arranged by size:
4187 4001 582 72
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6650 276
Size: 53 83 186 936 5116 2468
Fragments arranged by size:
5116 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BsiWI BsmBI BstXI BstZ17I EcoICRI FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI
PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI