Restriction map for
pFN21ASDA1219
(Circular) (Six-base) MAPSORT of: pFN21ASDA1219.seq Check: 2551 from: 1 to: 9202
pFN21ASDA1219 9202 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2140 2140
Size: 9202
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3263 7012 278
Size: 53 83 186 936 1727 3749 2468
Fragments arranged by size:
3749 2468 1727 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5401 6545 1937
Size: 3464 1144 4594
Fragments arranged by size:
4594 3464 1144
Acc65I G'GTAC_C
Cuts at: 2159 6523 2159
Size: 4364 4838
AclI AA'CG_TT
Cuts at: 1563 7329 7702 1563
Size: 5766 373 3063
Fragments arranged by size:
5766 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2151 4016 4178 4728 5118 5187 5399 5745
Size: 519 1865 162 550 390 69 212 346
Cuts at: 5745 7265 8649 8917 1632
Size: 1520 1384 268 1917
Fragments arranged by size:
1917 1865 1520 1384 550 519 390 346
268 212 162 69
AflII C'TTAA_G
Cuts at: 820 1017 4255 4580 820
Size: 197 3238 325 5442
Fragments arranged by size:
5442 3238 325 197
AflIII A'CryG_T
Cuts at: 1203 2886 8101 9078 1203
Size: 1683 5215 977 1327
Fragments arranged by size:
5215 1683 1327 977
AgeI A'CCGG_T
Cuts at: 8033 8033
Size: 9202
AhdI GACnn_n'nnGTC
Cuts at: 7931 7931
Size: 9202
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9202
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9202
AlwNI CAG_nnn'CTG
Cuts at: 2174 2455 2473 4277 6945 8517 2174
Size: 281 18 1804 2668 1572 2859
Fragments arranged by size:
2859 2668 1804 1572 281 18
ApaLI G'TGCA_C
Cuts at: 2748 4441 7259 8415 2748
Size: 1693 2818 1156 3535
Fragments arranged by size:
3535 2818 1693 1156
ApoI r'AATT_y
Cuts at: 1493 2185 2942 3533 3821 5321 5591 5895 6228
Size: 692 757 591 288 1500 270 304 333
Cuts at: 6228 6248 6511 6737 6889 8998 1493
Size: 20 263 226 152 2109 1697
Fragments arranged by size:
2109 1697 1500 757 692 591 333 304
288 270 263 226 152 20
AseI AT'TA_AT
Cuts at: 160 1032 3867 4019 5673 7756 160
Size: 872 2835 152 1654 2083 1606
Fragments arranged by size:
2835 2083 1654 1606 872 152
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9202
AvaI C'yCGr_G
Cuts at: 1870 1943 2499 6527 1870
Size: 73 556 4028 4545
Fragments arranged by size:
4545 4028 556 73
BamHI G'GATC_C
Cuts at: 6532 6899 6532
Size: 367 8835
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2159 3177 4025 4332 6523
Size: 325 217 627 372 1018 848 307 2191
Cuts at: 6523 7978 618
Size: 1455 1842
Fragments arranged by size:
2191 1842 1455 1018 848 627 372 325
307 217
BanII G_rGCy'C
Cuts at: 1391 4218 4284 4371 6301 6521 1391
Size: 2827 66 87 1930 220 4072
Fragments arranged by size:
4072 2827 1930 220 87 66
BbeI G_GCGC'C
Cuts at: 4336 4336
Size: 9202
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3218 3611 4966 5553 928
Size: 971 1319 393 1355 587 4577
Fragments arranged by size:
4577 1355 1319 971 587 393
BbvCI CC'TCA_GC
Cuts at: 3564 3564
Size: 9202
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2801 2835 4285 4319 7393 7427 1903
Size: 34 864 34 1450 34 3074 34 3678
Fragments arranged by size:
3678 3074 1450 864 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3006 7094 8304 3006
Size: 4088 1210 3904
Fragments arranged by size:
4088 3904 1210
BclI T'GATC_A
Cuts at: 1548 2065 1548
Size: 517 8685
BfrBI ATG'CAT
Cuts at: 3065 4725 3065
Size: 1660 7542
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7813 136
Size: 107 122 71 631 605 6141 1525
Fragments arranged by size:
6141 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5131 6155 9197 5131
Size: 1024 3042 5136
Fragments arranged by size:
5136 3042 1024
BlpI GC'TnA_GC
Cuts at: 6619 6619
Size: 9202
Bme1580I G_kGCm'C
Cuts at: 1790 2752 4445 4975 7263 8419 1790
Size: 962 1693 530 2288 1156 2573
Fragments arranged by size:
2573 2288 1693 1156 962 530
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9202
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4321 4616 5036
Size: 416 53 83 399 24 2904 295 420
Cuts at: 5036 5279 5655 5970 6933 7891 442
Size: 243 376 315 963 958 1753
Fragments arranged by size:
2904 1753 963 958 420 416 399 376
315 295 243 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3459 5742 1056
Size: 2403 2283 4516
Fragments arranged by size:
4516 2403 2283
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4260 4390 4260
Size: 130 9072
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2722 2820 3630 4471 4613 4798 6458 7862
Size: 1483 98 810 841 142 185 1660 1404
Cuts at: 7862 1239
Size: 2579
Fragments arranged by size:
2579 1660 1483 1404 841 810 185 142
98
Bpu10I CC'TnA_GC
Cuts at: 3564 3564
Size: 9202
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4230 5258 5942 6464 7327 8192 8490 4230
Size: 1028 684 522 863 865 298 4942
Fragments arranged by size:
4942 1028 865 863 684 522 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3325 4231 4407 4787 7865 882
Size: 617 1826 906 176 380 3078 2219
Fragments arranged by size:
3078 2219 1826 906 617 380 176
BsaAI yAC'GTr
Cuts at: 493 1204 6499 6993 493
Size: 711 5295 494 2702
Fragments arranged by size:
5295 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 9196 9196
Size: 9202
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3260 4333 7009 7391
Size: 53 83 186 936 1727 1073 2676 382
Cuts at: 7391 8026 275
Size: 635 1451
Fragments arranged by size:
2676 1727 1451 1073 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3171 4416 4845 6219 6423 7635 8033 8307 8454
Size: 1245 429 1374 204 1212 398 274 147
Cuts at: 8454 8865 3171
Size: 411 3508
Fragments arranged by size:
3508 1374 1245 1212 429 411 398 274
204 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2253 2283 6431 6461 1178
Size: 30 165 30 850 30 4148 30 3919
Fragments arranged by size:
4148 3919 850 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3264 3714 4361 5013 5267 5397 1186
Size: 2078 450 647 652 254 130 4991
Fragments arranged by size:
4991 2078 652 647 450 254 130
BseYI C'CCAG_C
Cuts at: 2713 2917 4277 5211 8405 8901 2713
Size: 204 1360 934 3194 496 3014
Fragments arranged by size:
3194 3014 1360 934 496 204
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2072 4481 1163
Size: 573 336 2409 5884
Fragments arranged by size:
5884 2409 573 336
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6652 7413 8441 1540
Size: 275 186 4651 761 1028 2301
Fragments arranged by size:
4651 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2752 3103 4371 4445 4551 6301 7263 7348 8419
Size: 351 1268 74 106 1750 962 85 1071
Cuts at: 8419 2752
Size: 3535
Fragments arranged by size:
3535 1750 1268 1071 962 351 106 85
74
BsmI GAATG_Cn'
Cuts at: 2979 3065 4296 5139 6713 6806 2979
Size: 86 1231 843 1574 93 5375
Fragments arranged by size:
5375 1574 1231 843 93 86
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2752 3103 4218 4284 4371 4445 4551
Size: 399 962 351 1115 66 87 74 106
Cuts at: 4551 4975 6301 6521 7263 7348 8419 1391
Size: 424 1326 220 742 85 1071 2174
Fragments arranged by size:
2174 1326 1115 1071 962 742 424 399
351 220 106 87 85 74 66
BspEI T'CCGG_A
Cuts at: 6219 8865 6219
Size: 2646 6556
BspHI T'CATG_A
Cuts at: 7091 8821 7091
Size: 1730 7472
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2140 4249 5395 6557 844
Size: 1296 2109 1146 1162 3489
Fragments arranged by size:
3489 2109 1296 1162 1146
BsrBI CCG'CTC
Cuts at: 1394 1823 2970 7089 1394
Size: 429 1147 4119 3507
Fragments arranged by size:
4119 3507 1147 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2336 2580 4083 4887 7697 7871 65
Size: 1183 1088 244 1503 804 2810 174 1396
Fragments arranged by size:
2810 1503 1396 1183 1088 804 244 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7846 8033 1700
Size: 110 6036 187 2869
Fragments arranged by size:
6036 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9202
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9202
BssSI C'ACGA_G
Cuts at: 7262 8274 7262
Size: 1012 8190
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9202
BstBI TT'CG_AA
Cuts at: 2007 8006 2007
Size: 5999 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9202
BstXI CCAn_nnnn'nTGG
Cuts at: 2689 4510 6473 2689
Size: 1821 1963 5418
Fragments arranged by size:
5418 1963 1821
BstYI r'GATC_y
Cuts at: 1972 3857 4492 5131 5467 5696 5881 6155 6532
Size: 1885 635 639 336 229 185 274 377
Cuts at: 6532 6899 7284 7301 8753 8839 8851 9197 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1885 1452 639 635 385 377 367
346 336 274 229 185 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 6000 6000
Size: 9202
BtgI C'CryG_G
Cuts at: 513 1065 2220 3501 513
Size: 552 1155 1281 6214
Fragments arranged by size:
6214 1281 1155 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8463
Fragments arranged by size:
8463 683 56
BtsI GCAGTG_nn'
Cuts at: 2042 2724 2735 3314 6718 7512 7532 2042
Size: 682 11 579 3404 794 20 3712
Fragments arranged by size:
3712 3404 794 682 579 20 11
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9202
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9167
DraI TTT'AAA
Cuts at: 2803 5068 5547 6204 6506 2803
Size: 2265 479 657 302 5499
Fragments arranged by size:
5499 2265 657 479 302
DraIII CAC_nnn'GTG
Cuts at: 4760 4760
Size: 9202
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8209 809
Size: 507 6893 1802
Fragments arranged by size:
6893 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3311 3702 4469
Size: 54 1425 50 275 18 1481 391 767
Cuts at: 4469 5087 6649 7538 9167 8
Size: 618 1562 889 1629 43
Fragments arranged by size:
1629 1562 1481 1425 889 767 618 391
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6649 1537
Size: 275 4837 4090
Fragments arranged by size:
4837 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3553 3609 3967 4154 5094 5172 5261 7132
Size: 2200 56 358 187 940 78 89 1871
Cuts at: 7132 1353
Size: 3423
Fragments arranged by size:
3423 2200 1871 940 358 187 89 78
56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6374 7789 8163 8309 387
Size: 1064 138 4785 1415 374 146 1280
Fragments arranged by size:
4785 1415 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4369 6299 4369
Size: 1930 7272
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2151 2722 2820 3630 4016 4178 4471
Size: 393 519 571 98 810 386 162 293
Cuts at: 4471 4613 4728 4798 5118 5187 5399 5745 6458
Size: 142 115 70 320 69 212 346 713
Cuts at: 6458 7265 7862 8649 8917 1239
Size: 807 597 787 268 1524
Fragments arranged by size:
1524 810 807 787 713 597 571 519
393 386 346 320 293 268 212 162
142 115 98 70 69
EcoNI CCTnn'n_nnAGG
Cuts at: 4385 5962 4385
Size: 1577 7625
EcoO109I rG'GnC_Cy
Cuts at: 2452 2452
Size: 9202
EcoRI G'AATT_C
Cuts at: 5321 5895 6511 6889 5321
Size: 574 616 378 7634
Fragments arranged by size:
7634 616 574 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4983 4983
Size: 9202
FspI TGC'GCA
Cuts at: 7708 7708
Size: 9202
HaeII r_GCGC'y
Cuts at: 1678 4336 8349 1678
Size: 2658 4013 2531
Fragments arranged by size:
4013 2658 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2024 2056 3944 3976 4153
Size: 32 135 32 452 32 1888 32 177
Cuts at: 4153 4185 4484 4516 5956 5988 7843 7875 7917
Size: 32 299 32 1440 32 1855 32 42
Cuts at: 7917 7949 1373
Size: 32 2626
Fragments arranged by size:
2626 1888 1855 1440 452 299 177 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3343 4405 6415 6546 6792 669
Size: 988 281 1405 1062 2010 131 246 3079
Fragments arranged by size:
3079 2010 1405 1062 988 281 246 131
HindIII A'AGCT_T
Cuts at: 748 5018 748
Size: 4270 4932
HpaI GTT'AAC
Cuts at: 3343 6792 3343
Size: 3449 5753
KasI G'GCGC_C
Cuts at: 4332 4332
Size: 9202
KpnI G_GTAC'C
Cuts at: 2163 6527 2163
Size: 4364 4838
MfeI C'AATT_G
Cuts at: 6801 6801
Size: 9202
MluI A'CGCG_T
Cuts at: 9078 9078
Size: 9202
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2089 3866 5041 5621 6454 8316 8500 1305
Size: 784 1777 1175 580 833 1862 184 2007
Fragments arranged by size:
2007 1862 1777 1175 833 784 580 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3704 10
Size: 54 1425 343 1872 5508
Fragments arranged by size:
5508 1872 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2079 2866 4061 4508 7160 7519 7678
Size: 648 913 787 1195 447 2652 359 159
Cuts at: 7678 518
Size: 2042
Fragments arranged by size:
2652 2042 1195 913 787 648 447 359
159
MspA1I CmG'CkG
Cuts at: 1606 3051 4274 6436 6618 7295 8443 8688 1606
Size: 1445 1223 2162 182 677 1148 245 2120
Fragments arranged by size:
2162 2120 1445 1223 1148 677 245 182
NarI GG'CG_CC
Cuts at: 4333 4333
Size: 9202
NcoI C'CATG_G
Cuts at: 513 1065 2220 3501 513
Size: 552 1155 1281 6214
Fragments arranged by size:
6214 1281 1155 552
NdeI CA'TA_TG
Cuts at: 387 2779 387
Size: 2392 6810
NheI G'CTAG_C
Cuts at: 1052 3455 5738 1052
Size: 2403 2283 4516
Fragments arranged by size:
4516 2403 2283
NotI GC'GGCC_GC
Cuts at: 6649 6649
Size: 9202
NsiI A_TGCA'T
Cuts at: 3067 4727 3067
Size: 1660 7542
NspI r_CATG'y
Cuts at: 1132 1229 2890 3652 4725 5654 6560 8105 1132
Size: 97 1661 762 1073 929 906 1545 2229
Fragments arranged by size:
2229 1661 1545 1073 929 906 762 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9202
PciI A'CATG_T
Cuts at: 2886 8101 2886
Size: 5215 3987
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2258 1108
Size: 1150 8052
PfoI T'CCnGG_A
Cuts at: 3930 6465 3930
Size: 2535 6667
PmeI GTTT'AAAC
Cuts at: 6506 6506
Size: 9202
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7271 7303 8784 8816 7271
Size: 32 1481 32 7657
Fragments arranged by size:
7657 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2452 2452
Size: 9202
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9202
PsiI TTA'TAA
Cuts at: 2927 6772 2927
Size: 3845 5357
PstI C_TGCA'G
Cuts at: 830 2538 6491 6554 830
Size: 1708 3953 63 3478
Fragments arranged by size:
3953 3478 1708 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9202
PvuII CAG'CTG
Cuts at: 3051 4274 6436 3051
Size: 1223 2162 5817
Fragments arranged by size:
5817 2162 1223
SacI G_AGCT'C
Cuts at: 4371 6301 4371
Size: 1930 7272
SalI G'TCGA_C
Cuts at: 1936 6544 1936
Size: 4608 4594
SapI GCTCTTCn'nnn_
Cuts at: 3553 3967 3553
Size: 414 8788
SbfI CC_TGCA'GG
Cuts at: 6554 6554
Size: 9202
ScaI AGT'ACT
Cuts at: 3229 4590 6115 7450 9162 3229
Size: 1361 1525 1335 1712 3269
Fragments arranged by size:
3269 1712 1525 1361 1335
SfcI C'TryA_G
Cuts at: 826 1045 2130 2390 2534 6045 6367 6487 6550
Size: 219 1085 260 144 3511 322 120 63
Cuts at: 6550 7685 8366 8557 826
Size: 1135 681 191 1471
Fragments arranged by size:
3511 1471 1135 1085 681 322 260 219
191 144 120 63
SfoI GGC'GCC
Cuts at: 4334 4334
Size: 9202
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9202
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9202
SmaI CCC'GGG
Cuts at: 1872 6529 1872
Size: 4657 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4245 4255 4580 5237 5921 6479
Size: 197 926 2302 10 325 657 684 558
Cuts at: 6479 7306 8207 8469 820
Size: 827 901 262 1553
Fragments arranged by size:
2302 1553 926 901 827 684 657 558
325 262 197 10
SnaBI TAC'GTA
Cuts at: 493 6499 6993 493
Size: 6006 494 2702
Fragments arranged by size:
6006 2702 494
SpeI A'CTAG_T
Cuts at: 152 3296 152
Size: 3144 6058
SphI G_CATG'C
Cuts at: 1132 6560 1132
Size: 5428 3774
SspI AAT'ATT
Cuts at: 5 52 2276 3024 4087 5072 7126 5
Size: 47 2224 748 1063 985 2054 2081
Fragments arranged by size:
2224 2081 2054 1063 985 748 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9202
StyI C'CwwG_G
Cuts at: 513 1065 2220 2682 3501 5605 6022 6641 513
Size: 552 1155 462 819 2104 417 619 3074
Fragments arranged by size:
3074 2104 1155 819 619 552 462 417
SwaI ATTT'AAAT
Cuts at: 5068 5068
Size: 9202
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2727 4073 4127 4325 6043 7229 7246
Size: 1255 1147 1346 54 198 1718 1186 17
Cuts at: 7246 7399 7584 8029 325
Size: 153 185 445 1498
Fragments arranged by size:
1718 1498 1346 1255 1186 1147 445 198
185 153 54 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2025 2642 3227
Size: 274 80 33 51 1443 48 617 585
Cuts at: 3227 4588 4653 6113 7448 9160 96
Size: 1361 65 1460 1335 1712 138
Fragments arranged by size:
1712 1460 1443 1361 1335 617 585 274
138 80 65 51 48 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2103 2135 2837 2869 3245 3277 9162 9194 2103
Size: 32 702 32 376 32 5885 32 2111
Fragments arranged by size:
5885 2111 702 376 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 6410 1316
Size: 5094 4108
XbaI T'CTAG_A
Cuts at: 3080 4363 6538 3080
Size: 1283 2175 5744
Fragments arranged by size:
5744 2175 1283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2470 2689 3499 2470
Size: 219 810 8173
Fragments arranged by size:
8173 810 219
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9202
XmaI C'CCGG_G
Cuts at: 1870 6527 1870
Size: 4657 4545
XmnI GAAnn'nnTTC
Cuts at: 3618 6081 7331 3618
Size: 2463 1250 5489
Fragments arranged by size:
5489 2463 1250
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3261 7010 276
Size: 53 83 186 936 1727 3749 2468
Fragments arranged by size:
3749 2468 1727 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BsiWI BsmBI
BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SrfI