Restriction map for
pFN21ASDA1206
(Circular) (Six-base) MAPSORT of: pFN21ASDA1206.seq Check: 7388 from: 1 to: 10456
pFN21ASDA1206 10456 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2413 3507 2413
Size: 1094 9362
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8266 278
Size: 53 83 186 936 6730 2468
Fragments arranged by size:
6730 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6699 7799 1937
Size: 4762 1100 4594
Fragments arranged by size:
4762 4594 1100
Acc65I G'GTAC_C
Cuts at: 7777 7777
Size: 10456
AclI AA'CG_TT
Cuts at: 1563 8583 8956 1563
Size: 7020 373 3063
Fragments arranged by size:
7020 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3215 8519 9903 10171 1632
Size: 1583 5304 1384 268 1917
Fragments arranged by size:
5304 1917 1583 1384 268
AfeI AGC'GCT
Cuts at: 6255 6255
Size: 10456
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10259
AflIII A'CryG_T
Cuts at: 1203 4132 9355 10332 1203
Size: 2929 5223 977 1327
Fragments arranged by size:
5223 2929 1327 977
AgeI A'CCGG_T
Cuts at: 4553 9287 4553
Size: 4734 5722
AhdI GACnn_n'nnGTC
Cuts at: 7281 9185 7281
Size: 1904 8552
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 10456
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 6641 7067 1184
Size: 5457 426 4573
Fragments arranged by size:
5457 4573 426
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6542 6574 6542
Size: 32 10424
AlwNI CAG_nnn'CTG
Cuts at: 2380 2407 3175 3190 3568 3594 3613 3942 4345
Size: 27 768 15 378 26 19 329 403
Cuts at: 4345 4413 5872 6568 6922 7687 8199 9771 2380
Size: 68 1459 696 354 765 512 1572 3065
Fragments arranged by size:
3065 1572 1459 768 765 696 512 403
378 354 329 68 27 26 19 15
ApaI G_GGCC'C
Cuts at: 2155 2782 2788 2942 3044 3750 4485 4737 4835
Size: 627 6 154 102 706 735 252 98
Cuts at: 4835 5212 5735 6594 2155
Size: 377 523 859 6017
Fragments arranged by size:
6017 859 735 706 627 523 377 252
154 102 98 6
ApaLI G'TGCA_C
Cuts at: 5585 6326 8513 9669 5585
Size: 741 2187 1156 6372
Fragments arranged by size:
6372 2187 1156 741
ApoI r'AATT_y
Cuts at: 1493 7765 7991 8143 10252 1493
Size: 6272 226 152 2109 1697
Fragments arranged by size:
6272 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 9010 160
Size: 872 7978 1606
Fragments arranged by size:
7978 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10456
AvaI C'yCGr_G
Cuts at: 1870 1943 2258 2270 2527 2781 2908 3116 3200
Size: 73 315 12 257 254 127 208 84
Cuts at: 3200 6593 6642 7461 7781 1870
Size: 3393 49 819 320 4545
Fragments arranged by size:
4545 3393 819 320 315 257 254 208
127 84 73 49 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 7387 7420 7387
Size: 33 10423
BamHI G'GATC_C
Cuts at: 6003 7786 8153 6003
Size: 1783 367 8306
Fragments arranged by size:
8306 1783 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2200 2451 2554 2620 2912
Size: 325 217 627 413 251 103 66 292
Cuts at: 2912 2948 2990 3784 3957 4099 4724 4847 5132
Size: 36 42 794 173 142 625 123 285
Cuts at: 5132 5473 6707 6933 6996 7777 9232 618
Size: 341 1234 226 63 781 1455 1842
Fragments arranged by size:
1842 1455 1234 794 781 627 625 413
341 325 292 285 251 226 217 173
142 123 103 66 63 42 36
BanII G_rGCy'C
Cuts at: 1391 2155 2782 2788 2942 3044 3750 4305 4485
Size: 764 627 6 154 102 706 555 180
Cuts at: 4485 4548 4595 4668 4737 4835 5088 5212 5251
Size: 63 47 73 69 98 253 124 39
Cuts at: 5251 5354 5374 5735 6594 6882 7523 7566 7775
Size: 103 20 361 859 288 641 43 209
Cuts at: 7775 1391
Size: 4072
Fragments arranged by size:
4072 859 764 706 641 627 555 361
288 253 209 180 154 124 103 102
98 73 69 63 47 43 39 20
6
BbeI G_GCGC'C
Cuts at: 2455 2455
Size: 10456
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5380 5621 6801 7280 928
Size: 971 3481 241 1180 479 4104
Fragments arranged by size:
4104 3481 1180 971 479 241
BbvCI CC'TCA_GC
Cuts at: 2487 5480 5978 6888 2487
Size: 2993 498 910 6055
Fragments arranged by size:
6055 2993 910 498
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5017 5051 5477 5511 5633 5667 8647
Size: 34 3080 34 426 34 122 34 2980
Cuts at: 8647 8681 1903
Size: 34 3678
Fragments arranged by size:
3678 3080 2980 426 122 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3420 5285 8348 9558 3420
Size: 1865 3063 1210 4318
Fragments arranged by size:
4318 3063 1865 1210
BclI T'GATC_A
Cuts at: 1548 7642 1548
Size: 6094 4362
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2601 2946 3048
Size: 107 122 71 631 605 929 345 102
Cuts at: 3048 3896 3905 4002 6088 9067 136
Size: 848 9 97 2086 2979 1525
Fragments arranged by size:
2979 2086 1525 929 848 631 605 345
122 107 102 97 71 9
BglII A'GATC_T
Cuts at: 10451 10451
Size: 10456
BlpI GC'TnA_GC
Cuts at: 2252 3349 4811 7560 7873 2252
Size: 1097 1462 2749 313 4835
Fragments arranged by size:
4835 2749 1462 1097 313
Bme1580I G_kGCm'C
Cuts at: 1790 2155 2322 2345 2559 2782 2788 2915 2942
Size: 365 167 23 214 223 6 127 27
Cuts at: 2942 2951 3044 3246 3750 3825 3962 4314 4485
Size: 9 93 202 504 75 137 352 171
Cuts at: 4485 4493 4737 4835 4850 5135 5212 5589 5735
Size: 8 244 98 15 285 77 377 146
Cuts at: 5735 6330 6594 6710 6938 7127 7660 8517 9673
Size: 595 264 116 228 189 533 857 1156
Cuts at: 9673 1790
Size: 2573
Fragments arranged by size:
2573 1156 857 595 533 504 377 365
352 285 264 244 228 223 214 202
189 171 167 146 137 127 116 98
93 77 75 27 23 15 9 8
6
BmgBI CAC'GTC
Cuts at: 1318 3084 3814 3847 4683 5146 6553 1318
Size: 1766 730 33 836 463 1407 5221
Fragments arranged by size:
5221 1766 1407 836 730 463 33
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3442 4301 4740
Size: 416 53 83 399 24 2025 859 439
Cuts at: 4740 5082 7433 8187 9145 442
Size: 342 2351 754 958 1753
Fragments arranged by size:
2351 2025 1753 958 859 754 439 416
399 342 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 6254 7229 1056
Size: 5198 975 4283
Fragments arranged by size:
5198 4283 975
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2234 2818 6775 7564 2234
Size: 584 3957 789 5126
Fragments arranged by size:
5126 3957 789 584
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2650 3197 3215 3327 3458 3494 3783 4208
Size: 1411 547 18 112 131 36 289 425
Cuts at: 4208 4218 4247 4862 4952 5198 5924 5933 6011
Size: 10 29 615 90 246 726 9 78
Cuts at: 6011 6212 6290 6983 7619 7682 9116 1239
Size: 201 78 693 636 63 1434 2579
Fragments arranged by size:
2579 1434 1411 726 693 636 615 547
425 289 246 201 131 112 90 78
78 63 36 29 18 10 9
Bpu10I CC'TnA_GC
Cuts at: 2487 4662 5480 5978 6760 6888 2487
Size: 2175 818 498 782 128 6055
Fragments arranged by size:
6055 2175 818 782 498 128
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4759 5498 6770 8581 9446 9744 4759
Size: 739 1272 1811 865 298 5471
Fragments arranged by size:
5471 1811 1272 865 739 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2036 2658 5909 5922 6356 9119 882
Size: 617 537 622 3251 13 434 2763 2219
Fragments arranged by size:
3251 2763 2219 622 617 537 434 13
BsaAI yAC'GTr
Cuts at: 493 1204 2809 2851 3754 6325 7753 8247 493
Size: 711 1605 42 903 2571 1428 494 2702
Fragments arranged by size:
2702 2571 1605 1428 903 711 494 42
BsaBI GATnn'nnATC
Cuts at: 10450 10450
Size: 10456
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2452 2742 3078 6495
Size: 53 83 186 936 919 290 336 3417
Cuts at: 6495 7362 8263 8645 9280 275
Size: 867 901 382 635 1451
Fragments arranged by size:
3417 1451 936 919 901 867 635 382
336 290 186 83 53
BsaWI w'CCGG_w
Cuts at: 3423 4553 4945 7087 8889 9287 9561 9708 10119
Size: 1130 392 2142 1802 398 274 147 411
Cuts at: 10119 3423
Size: 3760
Fragments arranged by size:
3760 2142 1802 1130 411 398 392 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2732 2762 2828 2858 4499
Size: 30 165 30 1329 30 66 30 1641
Cuts at: 4499 4529 4925 4955 5227 5257 6671 6701 6788
Size: 30 396 30 272 30 1414 30 87
Cuts at: 6788 6818 6858 6888 7570 7600 1178
Size: 30 40 30 682 30 4034
Fragments arranged by size:
4034 1641 1414 1329 682 396 272 165
87 66 40 30 30 30 30 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 3596 4244 5251 5327 5588 5822 5825
Size: 913 1497 648 1007 76 261 234 3
Cuts at: 5825 6161 6209 6989 7031 7679 1186
Size: 336 48 780 42 648 3963
Fragments arranged by size:
3963 1497 1007 913 780 648 648 336
261 234 76 48 42 3
BseYI C'CCAG_C
Cuts at: 2332 2352 2986 3044 3149 3327 4110 4293 4526
Size: 20 634 58 105 178 783 183 233
Cuts at: 4526 4827 4851 5022 5067 5211 6203 6301 9659
Size: 301 24 171 45 144 992 98 3358
Cuts at: 9659 10155 2332
Size: 496 2633
Fragments arranged by size:
3358 2633 992 783 634 496 301 233
183 178 171 144 105 98 58 45
24 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2039 3584 4643 5171 5216 5723 5738
Size: 573 303 1545 1059 528 45 507 15
Cuts at: 5738 5759 5918 6878 7063 7247 1163
Size: 21 159 960 185 184 4372
Fragments arranged by size:
4372 1545 1059 960 573 528 507 303
185 184 159 45 21 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2569 3037 5596 7906 8667 9695
Size: 275 186 568 468 2559 2310 761 1028
Cuts at: 9695 1540
Size: 2301
Fragments arranged by size:
2559 2310 2301 1028 761 568 468 275
186
BsiHKAI G_wGCw'C
Cuts at: 3144 3993 4305 5306 5589 5832 6330 6651 7523
Size: 849 312 1001 283 243 498 321 872
Cuts at: 7523 7566 8517 8602 9673 3144
Size: 43 951 85 1071 3927
Fragments arranged by size:
3927 1071 1001 951 872 849 498 321
312 283 243 85 43
BsmI GAATG_Cn'
Cuts at: 7312 7967 8060 7312
Size: 655 93 9708
Fragments arranged by size:
9708 655 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2155 2322 2345 2559 2782 2788 2915
Size: 399 365 167 23 214 223 6 127
Cuts at: 2915 2942 2951 3044 3144 3246 3750 3825 3962
Size: 27 9 93 100 102 504 75 137
Cuts at: 3962 3993 4305 4314 4485 4493 4548 4595 4668
Size: 31 312 9 171 8 55 47 73
Cuts at: 4668 4737 4835 4850 5088 5135 5212 5251 5306
Size: 69 98 15 238 47 77 39 55
Cuts at: 5306 5354 5374 5589 5735 5832 6330 6594 6651
Size: 48 20 215 146 97 498 264 57
Cuts at: 6651 6710 6882 6938 7127 7523 7566 7660 7775
Size: 59 172 56 189 396 43 94 115
Cuts at: 7775 8517 8602 9673 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 504 498 399 396 365
312 264 238 223 215 214 189 172
171 167 146 137 127 115 102 100
98 97 94 93 85 77 75 73
69 59 57 56 55 55 48 47
47 43 39 31 27 23 20 15
9 9 8 6
BspEI T'CCGG_A
Cuts at: 4945 7087 10119 4945
Size: 2142 3032 5282
Fragments arranged by size:
5282 3032 2142
BspHI T'CATG_A
Cuts at: 5958 6288 8345 10075 5958
Size: 330 2057 1730 6339
Fragments arranged by size:
6339 2057 1730 330
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2413 3375 3507 4076 6156 7702 7811 844
Size: 1569 962 132 569 2080 1546 109 3489
Fragments arranged by size:
3489 2080 1569 1546 962 569 132 109
BsrBI CCG'CTC
Cuts at: 1394 1823 2748 2802 8343 1394
Size: 429 925 54 5541 3507
Fragments arranged by size:
5541 3507 925 429 54
BsrDI GCAATG_nn'
Cuts at: 65 1248 3156 5719 8951 9125 65
Size: 1183 1908 2563 3232 174 1396
Fragments arranged by size:
3232 2563 1908 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3208 4216 4553 4647 5135 5918 7176
Size: 110 1398 1008 337 94 488 783 1258
Cuts at: 7176 9100 9287 1700
Size: 1924 187 2869
Fragments arranged by size:
2869 1924 1398 1258 1008 783 488 337
187 110 94
BsrGI T'GTAC_A
Cuts at: 96 3282 5847 6444 96
Size: 3186 2565 597 4108
Fragments arranged by size:
4108 3186 2565 597
BssHII G'CGCG_C
Cuts at: 1925 6037 1925
Size: 4112 6344
BssSI C'ACGA_G
Cuts at: 8516 9528 8516
Size: 1012 9444
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4413 5872 6634 794
Size: 3619 1459 762 4616
Fragments arranged by size:
4616 3619 1459 762
BstBI TT'CG_AA
Cuts at: 2007 9260 2007
Size: 7253 3203
BstEII G'GTnAC_C
Cuts at: 1184 6305 6773 1184
Size: 5121 468 4867
Fragments arranged by size:
5121 4867 468
BstXI CCAn_nnnn'nTGG
Cuts at: 2596 3239 4049 4886 5285 2596
Size: 643 810 837 399 7767
Fragments arranged by size:
7767 837 810 643 399
BstYI r'GATC_y
Cuts at: 1972 6003 7467 7786 8153 8538 8555 10007 10093
Size: 4031 1464 319 367 385 17 1452 86
Cuts at: 10093 10105 10451 1972
Size: 12 346 1977
Fragments arranged by size:
4031 1977 1464 1452 385 367 346 319
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4563 6084 6419 4563
Size: 1521 335 8600
Fragments arranged by size:
8600 1521 335
BtgI C'CryG_G
Cuts at: 513 1065 2277 2773 2934 2976 3066 3219 3298
Size: 552 1212 496 161 42 90 153 79
Cuts at: 3298 4389 5596 5775 6055 6366 6489 6898 513
Size: 1091 1207 179 280 311 123 409 4071
Fragments arranged by size:
4071 1212 1207 1091 552 496 409 311
280 179 161 153 123 90 79 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2141 7174 7363 487
Size: 683 56 915 5033 189 3580
Fragments arranged by size:
5033 3580 915 683 189 56
BtsI GCAGTG_nn'
Cuts at: 2135 2238 3388 3592 3892 3973 3991 4177 4258
Size: 103 1150 204 300 81 18 186 81
Cuts at: 4258 4411 5030 5306 5817 7972 8766 8786 2135
Size: 153 619 276 511 2155 794 20 3805
Fragments arranged by size:
3805 2155 1150 794 619 511 300 276
204 186 153 103 81 81 20 18
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10456
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2124 2159 529
Size: 35 1560 35 8826
Fragments arranged by size:
8826 1560 35 35
DraI TTT'AAA
Cuts at: 7760 7760
Size: 10456
DraIII CAC_nnn'GTG
Cuts at: 3418 3979 4099 7003 3418
Size: 561 120 2904 6871
Fragments arranged by size:
6871 2904 561 120
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9463 809
Size: 507 8147 1802
Fragments arranged by size:
8147 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2206 2466 2566
Size: 54 1425 50 275 18 376 260 100
Cuts at: 2566 2586 2631 2646 3034 3163 4218 4422 4459
Size: 20 45 15 388 129 1055 204 37
Cuts at: 4459 4641 4746 4912 5014 5593 6052 6103 7903
Size: 182 105 166 102 579 459 51 1800
Cuts at: 7903 8792 10421 8
Size: 889 1629 43
Fragments arranged by size:
1800 1629 1425 1055 889 579 459 388
376 275 260 204 182 166 129 105
102 100 54 51 50 45 43 37
20 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2566 3034 5593 7903 1537
Size: 275 754 468 2559 2310 4090
Fragments arranged by size:
4090 2559 2310 754 468 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2249 6170 6458 6821 8386 1353
Size: 896 3921 288 363 1565 3423
Fragments arranged by size:
3921 3423 1565 896 363 288
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2627 2740 2756 5096 9043 9417
Size: 1064 138 1038 113 16 2340 3947 374
Cuts at: 9417 9563 387
Size: 146 1280
Fragments arranged by size:
3947 2340 1280 1064 1038 374 146 138
113 16
EcoICRI GAG'CTC
Cuts at: 4303 7521 7564 4303
Size: 3218 43 7195
Fragments arranged by size:
7195 3218 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2650 3197 3215 3327 3458 3494 3783
Size: 393 1018 547 18 112 131 36 289
Cuts at: 3783 4208 4218 4247 4862 4952 5198 5924 5933
Size: 425 10 29 615 90 246 726 9
Cuts at: 5933 6011 6212 6290 6983 7619 7682 8519 9116
Size: 78 201 78 693 636 63 837 597
Cuts at: 9116 9903 10171 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1018 837 787 726 693 636 615
597 547 425 393 289 268 246 201
131 112 90 78 78 63 36 29
18 10 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2208 2888 4566 6418 6422 7664 7709 2208
Size: 680 1678 1852 4 1242 45 4955
Fragments arranged by size:
4955 1852 1678 1242 680 45 4
EcoO109I rG'GnC_Cy
Cuts at: 2151 2491 2572 2674 2730 2778 2857 2881 3040
Size: 340 81 102 56 48 79 24 159
Cuts at: 3040 3041 3338 3484 3746 3747 4088 4482 4798
Size: 1 297 146 262 1 341 394 316
Cuts at: 4798 4831 4832 4971 5208 5221 5492 5548 5732
Size: 33 1 139 237 13 271 56 184
Cuts at: 5732 5869 6127 6294 6485 6590 6726 6856 7249
Size: 137 258 167 191 105 136 130 393
Cuts at: 7249 7257 7669 2151
Size: 8 412 4938
Fragments arranged by size:
4938 412 394 393 341 340 316 297
271 262 258 237 191 184 167 159
146 139 137 136 130 105 102 81
79 56 56 48 33 24 13 8
1 1 1
EcoRI G'AATT_C
Cuts at: 7765 8143 7765
Size: 378 10078
FspI TGC'GCA
Cuts at: 5655 8962 5655
Size: 3307 7149
HaeII r_GCGC'y
Cuts at: 1678 2455 2963 3219 4026 6257 6760 9603 1678
Size: 777 508 256 807 2231 503 2843 2531
Fragments arranged by size:
2843 2531 2231 807 777 508 503 256
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2732 2764 4804 4836 6788
Size: 32 135 32 1160 32 2040 32 1952
Cuts at: 6788 6820 7267 7299 9097 9129 9171 9203 1373
Size: 32 447 32 1798 32 42 32 2626
Fragments arranged by size:
2626 2040 1952 1798 1160 447 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5323 7800 8046 669
Size: 988 281 3385 2477 246 3079
Fragments arranged by size:
3385 3079 2477 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 10456
HpaI GTT'AAC
Cuts at: 8046 8046
Size: 10456
KasI G'GCGC_C
Cuts at: 2451 2451
Size: 10456
KpnI G_GTAC'C
Cuts at: 7781 7781
Size: 10456
MfeI C'AATT_G
Cuts at: 8055 8055
Size: 10456
MluI A'CGCG_T
Cuts at: 4132 10332 4132
Size: 6200 4256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2730 4620 6168 6669 7329 9570 9754 1305
Size: 1425 1890 1548 501 660 2241 184 2007
Fragments arranged by size:
2241 2007 1890 1548 1425 660 501 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2588 2633 4643 4748 4914
Size: 54 1425 343 756 45 2010 105 166
Cuts at: 4914 5016 6054 10
Size: 102 1038 4412
Fragments arranged by size:
4412 2010 1425 1038 756 343 166 105
102 54 45
MslI CAynn'nnrTG
Cuts at: 518 1166 3396 4043 5843 8414 8773 8932 518
Size: 648 2230 647 1800 2571 359 159 2042
Fragments arranged by size:
2571 2230 2042 1800 648 647 359 159
MspA1I CmG'CkG
Cuts at: 1606 2377 2644 2775 2936 3048 3280 3468 3490
Size: 771 267 131 161 112 232 188 22
Cuts at: 3490 3556 4297 4997 5155 5383 5598 5722 5761
Size: 66 741 700 158 228 215 124 39
Cuts at: 5761 5902 6000 6070 6892 7693 7872 8549 9697
Size: 141 98 70 822 801 179 677 1148
Cuts at: 9697 9942 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 822 801 771 741 700 677
267 245 232 228 215 188 179 161
158 141 131 124 112 98 70 66
39 22
NaeI GCC'GGC
Cuts at: 4218 4649 4218
Size: 431 10025
NarI GG'CG_CC
Cuts at: 2452 2452
Size: 10456
NcoI C'CATG_G
Cuts at: 513 1065 2976 4389 6055 6366 513
Size: 552 1911 1413 1666 311 4603
Fragments arranged by size:
4603 1911 1666 1413 552 311
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10456
NgoMIV G'CCGG_C
Cuts at: 4216 4647 4216
Size: 431 10025
NheI G'CTAG_C
Cuts at: 1052 6250 7225 1052
Size: 5198 975 4283
Fragments arranged by size:
5198 4283 975
NotI GC'GGCC_GC
Cuts at: 7903 7903
Size: 10456
NspI r_CATG'y
Cuts at: 1132 1229 6451 6740 7814 9359 1132
Size: 97 5222 289 1074 1545 2229
Fragments arranged by size:
5222 2229 1545 1074 289 97
PasI CC'CwG_GG
Cuts at: 1348 3271 3307 4055 4327 5728 7253 1348
Size: 1923 36 748 272 1401 1525 4551
Fragments arranged by size:
4551 1923 1525 1401 748 272 36
PciI A'CATG_T
Cuts at: 9355 9355
Size: 10456
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2065 2407 3175 3334 3418 3901 4033 4345
Size: 957 342 768 159 84 483 132 312
Cuts at: 4345 5530 6190 6922 7597 1108
Size: 1185 660 732 675 3967
Fragments arranged by size:
3967 1185 957 768 732 675 660 483
342 312 159 132 84
PfoI T'CCnGG_A
Cuts at: 3681 4929 5529 6012 7568 3681
Size: 1248 600 483 1556 6569
Fragments arranged by size:
6569 1556 1248 600 483
PmeI GTTT'AAAC
Cuts at: 7760 7760
Size: 10456
PmlI CAC'GTG
Cuts at: 3754 3754
Size: 10456
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8525 8557 10038 10070 8525
Size: 32 1481 32 8911
Fragments arranged by size:
8911 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2491 2572 2857 3484 4088 4798 5221 6294 6856
Size: 81 285 627 604 710 423 1073 562
Cuts at: 6856 7249 7257 7669 2491
Size: 393 8 412 5278
Fragments arranged by size:
5278 1073 710 627 604 562 423 412
393 285 81 8
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10456
PsiI TTA'TAA
Cuts at: 8026 8026
Size: 10456
PspOMI G'GGCC_C
Cuts at: 2151 2778 2784 2938 3040 3746 4481 4733 4831
Size: 627 6 154 102 706 735 252 98
Cuts at: 4831 5208 5731 6590 2151
Size: 377 523 859 6017
Fragments arranged by size:
6017 859 735 706 627 523 377 252
154 102 98 6
PspXI vC'TCGA_Gb
Cuts at: 2270 2270
Size: 10456
PstI C_TGCA'G
Cuts at: 830 2268 3887 4409 5301 5812 6075 7134 7692
Size: 1438 1619 522 892 511 263 1059 558
Cuts at: 7692 7710 7808 830
Size: 18 98 3478
Fragments arranged by size:
3478 1619 1438 1059 892 558 522 511
263 98 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10456
PvuII CAG'CTG
Cuts at: 2377 2644 3280 3468 3556 5722 5902 6892 7693
Size: 267 636 188 88 2166 180 990 801
Cuts at: 7693 2377
Size: 5140
Fragments arranged by size:
5140 2166 990 801 636 267 188 180
88
SacI G_AGCT'C
Cuts at: 4305 7523 7566 4305
Size: 3218 43 7195
Fragments arranged by size:
7195 3218 43
SacII CC_GC'GG
Cuts at: 2776 2937 5599 2776
Size: 161 2662 7633
Fragments arranged by size:
7633 2662 161
SalI G'TCGA_C
Cuts at: 1936 7798 1936
Size: 5862 4594
SanDI GG'GwC_CC
Cuts at: 7257 7257
Size: 10456
SapI GCTCTTCn'nnn_
Cuts at: 6170 6458 6170
Size: 288 10168
SbfI CC_TGCA'GG
Cuts at: 7808 7808
Size: 10456
ScaI AGT'ACT
Cuts at: 8704 10416 8704
Size: 1712 8744
SexAI A'CCwGG_T
Cuts at: 3414 3780 4095 6471 6999 3414
Size: 366 315 2376 528 6871
Fragments arranged by size:
6871 2376 528 366 315
SfcI C'TryA_G
Cuts at: 826 1045 2264 3883 4018 4405 5297 5808 6071
Size: 219 1219 1619 135 387 892 511 263
Cuts at: 6071 7130 7688 7706 7804 8939 9620 9811 826
Size: 1059 558 18 98 1135 681 191 1471
Fragments arranged by size:
1619 1471 1219 1135 1059 892 681 558
511 387 263 219 191 135 98 18
SfoI GGC'GCC
Cuts at: 2453 2453
Size: 10456
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10456
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10456
SmaI CCC'GGG
Cuts at: 1872 2529 2783 2910 6595 6644 7463 7783 1872
Size: 657 254 127 3685 49 819 320 4545
Fragments arranged by size:
4545 3685 819 657 320 254 127 49
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2270 4774 5513 6749 8560 9461
Size: 197 926 327 2504 739 1236 1811 901
Cuts at: 9461 9723 820
Size: 262 1553
Fragments arranged by size:
2504 1811 1553 1236 926 901 739 327
262 197
SnaBI TAC'GTA
Cuts at: 493 7753 8247 493
Size: 7260 494 2702
Fragments arranged by size:
7260 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10456
SphI G_CATG'C
Cuts at: 1132 6740 7814 1132
Size: 5608 1074 3774
Fragments arranged by size:
5608 3774 1074
SrfI GCCC'GGGC
Cuts at: 2783 2783
Size: 10456
SspI AAT'ATT
Cuts at: 5 52 8380 5
Size: 47 8328 2081
Fragments arranged by size:
8328 2081 47
StuI AGG'CCT
Cuts at: 1517 2595 3899 4080 4161 4251 6765 1517
Size: 1078 1304 181 81 90 2514 5208
Fragments arranged by size:
5208 2514 1304 1078 181 90 81
StyI C'CwwG_G
Cuts at: 513 1065 2455 2942 2976 3900 4389 5231 6055
Size: 552 1390 487 34 924 489 842 824
Cuts at: 6055 6366 6939 7032 7077 7895 513
Size: 311 573 93 45 818 3074
Fragments arranged by size:
3074 1390 924 842 824 818 573 552
489 487 311 93 45 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2966 2978 3284 4340 4370 4865 4898
Size: 1255 1386 12 306 1056 30 495 33
Cuts at: 4898 5645 5792 5987 6293 6683 7291 8483 8500
Size: 747 147 195 306 390 608 1192 17
Cuts at: 8500 8653 8838 9283 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1386 1255 1192 1056 747 608 495
445 390 306 306 195 185 153 147
33 30 17 12
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2841 3282 5637
Size: 274 80 33 51 1443 864 441 2355
Cuts at: 5637 5847 6444 8702 10414 96
Size: 210 597 2258 1712 138
Fragments arranged by size:
2355 2258 1712 1443 864 597 441 274
210 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3476 3508 4499 4531 4684 4716 4925 4957 5227
Size: 32 991 32 153 32 209 32 270
Cuts at: 5227 5259 6858 6890 7570 7602 10416 10448 3476
Size: 32 1599 32 680 32 2814 32 3484
Fragments arranged by size:
3484 2814 1599 991 680 270 209 153
32 32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3812 4802 6695 1316
Size: 2496 990 1893 5077
Fragments arranged by size:
5077 2496 1893 990
XbaI T'CTAG_A
Cuts at: 7792 7792
Size: 10456
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2641 2674 2983 4432 4903 5467 2641
Size: 33 309 1449 471 564 7630
Fragments arranged by size:
7630 1449 564 471 309 33
XhoI C'TCGA_G
Cuts at: 1943 2270 1943
Size: 327 10129
XmaI C'CCGG_G
Cuts at: 1870 2527 2781 2908 6593 6642 7461 7781 1870
Size: 657 254 127 3685 49 819 320 4545
Fragments arranged by size:
4545 3685 819 657 320 254 127 49
XmnI GAAnn'nnTTC
Cuts at: 8585 8585
Size: 10456
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8264 276
Size: 53 83 186 936 6730 2468
Fragments arranged by size:
6730 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BfrBI BsiWI BsmBI BstZ17I EcoRV FalI
FseI FspAI NruI NsiI PacI PsrI RsrII SfiI
SwaI