Restriction map for
pFN21ASDA1202
(Circular) (Six-base) MAPSORT of: pFN21ASDA1202.seq Check: 8681 from: 1 to: 9223
pFN21ASDA1202 9223 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3291 3668 3291
Size: 377 8846
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7033 278
Size: 53 83 186 936 5497 2468
Fragments arranged by size:
5497 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6493 6566 1937
Size: 4556 73 4594
Fragments arranged by size:
4594 4556 73
Acc65I G'GTAC_C
Cuts at: 6544 6544
Size: 9223
AclI AA'CG_TT
Cuts at: 1563 7350 7723 1563
Size: 5787 373 3063
Fragments arranged by size:
5787 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2376 2377 2406 2855 4556 5118 5811 6329
Size: 744 1 29 449 1701 562 693 518
Cuts at: 6329 7286 8670 8938 1632
Size: 957 1384 268 1917
Fragments arranged by size:
1917 1701 1384 957 744 693 562 518
449 268 29 1
AflII C'TTAA_G
Cuts at: 820 1017 2096 820
Size: 197 1079 7947
Fragments arranged by size:
7947 1079 197
AflIII A'CryG_T
Cuts at: 1203 2430 2562 8122 9099 1203
Size: 1227 132 5560 977 1327
Fragments arranged by size:
5560 1327 1227 977 132
AgeI A'CCGG_T
Cuts at: 8054 8054
Size: 9223
AhdI GACnn_n'nnGTC
Cuts at: 7952 7952
Size: 9223
AleI CACnn'nnGTG
Cuts at: 1166 3859 4653 1166
Size: 2693 794 5736
Fragments arranged by size:
5736 2693 794
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3621 1184
Size: 2437 6786
AlwNI CAG_nnn'CTG
Cuts at: 3271 4157 4677 6277 6966 8538 3271
Size: 886 520 1600 689 1572 3956
Fragments arranged by size:
3956 1600 1572 886 689 520
ApaI G_GGCC'C
Cuts at: 2820 2840 5836 6507 2820
Size: 20 2996 671 5536
Fragments arranged by size:
5536 2996 671 20
ApaLI G'TGCA_C
Cuts at: 3293 7280 8436 3293
Size: 3987 1156 4080
Fragments arranged by size:
4080 3987 1156
ApoI r'AATT_y
Cuts at: 1493 3807 4624 4919 6119 6532 6758 6910 9019
Size: 2314 817 295 1200 413 226 152 2109
Cuts at: 9019 1493
Size: 1697
Fragments arranged by size:
2314 2109 1697 1200 817 413 295 226
152
AseI AT'TA_AT
Cuts at: 160 1032 7777 160
Size: 872 6745 1606
Fragments arranged by size:
6745 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9223
AvaI C'yCGr_G
Cuts at: 1870 1943 3744 4859 5232 5617 6198 6458 6548
Size: 73 1801 1115 373 385 581 260 90
Cuts at: 6548 1870
Size: 4545
Fragments arranged by size:
4545 1801 1115 581 385 373 260 90
73
AvrII C'CTAG_G
Cuts at: 5938 5938
Size: 9223
BamHI G'GATC_C
Cuts at: 3125 6553 6920 3125
Size: 3428 367 5428
Fragments arranged by size:
5428 3428 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2077 3348 4488 4797 4876
Size: 325 217 627 290 1271 1140 309 79
Cuts at: 4876 5218 6544 7999 618
Size: 342 1326 1455 1842
Fragments arranged by size:
1842 1455 1326 1271 1140 627 342 325
309 290 217 79
BanII G_rGCy'C
Cuts at: 1391 2820 2840 2887 3086 3330 3702 3900 4469
Size: 1429 20 47 199 244 372 198 569
Cuts at: 4469 4708 5156 5262 5618 5836 6507 6542 1391
Size: 239 448 106 356 218 671 35 4072
Fragments arranged by size:
4072 1429 671 569 448 372 356 244
239 218 199 198 106 47 35 20
BbeI G_GCGC'C
Cuts at: 4492 5222 4492
Size: 730 8493
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4005 4967 928
Size: 971 2106 962 5184
Fragments arranged by size:
5184 2106 971 962
BbvCI CC'TCA_GC
Cuts at: 4025 4845 5594 4025
Size: 820 749 7654
Fragments arranged by size:
7654 820 749
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7414 7448 1903
Size: 34 5477 34 3678
Fragments arranged by size:
5477 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2798 7115 8325 2798
Size: 4317 1210 3696
Fragments arranged by size:
4317 3696 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9223
BfrBI ATG'CAT
Cuts at: 2374 4815 2374
Size: 2441 6782
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3081 7834 136
Size: 107 122 71 631 605 1409 4753 1525
Fragments arranged by size:
4753 1525 1409 631 605 122 107 71
BglII A'GATC_T
Cuts at: 9218 9218
Size: 9223
BlpI GC'TnA_GC
Cuts at: 5727 6640 5727
Size: 913 8310
Bme1580I G_kGCm'C
Cuts at: 1790 2080 2632 2820 2840 3259 3297 3351 4858
Size: 290 552 188 20 419 38 54 1507
Cuts at: 4858 5836 6507 7284 8440 1790
Size: 978 671 777 1156 2573
Fragments arranged by size:
2573 1507 1156 978 777 671 552 419
290 188 54 38 20
BmgBI CAC'GTC
Cuts at: 1318 2431 1318
Size: 1113 8110
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2697 5278 5356
Size: 416 53 83 399 24 1280 2581 78
Cuts at: 5356 5515 6954 7912 442
Size: 159 1439 958 1753
Fragments arranged by size:
2581 1753 1439 1280 958 416 399 159
83 78 53 24
BmtI G_CTAG'C
Cuts at: 1056 3197 1056
Size: 2141 7082
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2674 2906 3995 4224 5170 2674
Size: 232 1089 229 946 6727
Fragments arranged by size:
6727 1089 946 232 229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2363 2528 2835 2863 3815 4194 4453 5630
Size: 1124 165 307 28 952 379 259 1177
Cuts at: 5630 6461 7883 1239
Size: 831 1422 2579
Fragments arranged by size:
2579 1422 1177 1124 952 831 379 307
259 165 28
Bpu10I CC'TnA_GC
Cuts at: 3198 4025 4845 5594 5718 3198
Size: 827 820 749 124 6703
Fragments arranged by size:
6703 827 820 749 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2258 4284 5057 7348 8213 8511 2258
Size: 2026 773 2291 865 298 2970
Fragments arranged by size:
2970 2291 2026 865 773 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2720 3881 4212 4423 5273 6358 7886
Size: 617 1221 1161 331 211 850 1085 1528
Cuts at: 7886 882
Size: 2219
Fragments arranged by size:
2219 1528 1221 1161 1085 850 617 331
211
BsaAI yAC'GTr
Cuts at: 493 1204 6319 6520 7014 493
Size: 711 5115 201 494 2702
Fragments arranged by size:
5115 2702 711 494 201
BsaBI GATnn'nnATC
Cuts at: 9217 9217
Size: 9223
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4489 5219 7030 7412
Size: 53 83 186 936 2956 730 1811 382
Cuts at: 7412 8047 275
Size: 635 1451
Fragments arranged by size:
2956 1811 1451 936 730 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2265 7656 8054 8328 8475 8886 2265
Size: 5391 398 274 147 411 2602
Fragments arranged by size:
5391 2602 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4130 4160 4695 4725 1178
Size: 30 165 30 2727 30 535 30 5676
Fragments arranged by size:
5676 2727 535 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2305 2663 2882 3829 4138 5159 5313 5316
Size: 1119 358 219 947 309 1021 154 3
Cuts at: 5316 5325 5328 5444 5447 5450 5453 5456 5459
Size: 9 3 116 3 3 3 3 3
Cuts at: 5459 5492 5495 5498 5501 5510 5513 5516 5519
Size: 33 3 3 3 9 3 3 3
Cuts at: 5519 5522 5582 5960 6092 6458 1186
Size: 3 60 378 132 366 3951
Fragments arranged by size:
3951 1119 1021 947 378 366 358 309
219 154 132 116 60 33 9 9
3 3 3 3 3 3 3 3
3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2771 3825 3962 4304 5044 5376 5641 6030 6506
Size: 1054 137 342 740 332 265 389 476
Cuts at: 6506 8426 8922 2771
Size: 1920 496 3072
Fragments arranged by size:
3072 1920 1054 740 496 476 389 342
332 265 137
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2081 2459 3730 4934 5391 1163
Size: 573 345 378 1271 1204 457 4995
Fragments arranged by size:
4995 1271 1204 573 457 378 345
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6673 7434 8462 1540
Size: 275 186 4672 761 1028 2301
Fragments arranged by size:
4672 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3297 3330 4469 4708 5156 5262 5557 6318 7284
Size: 33 1139 239 448 106 295 761 966
Cuts at: 7284 7369 8440 3297
Size: 85 1071 4080
Fragments arranged by size:
4080 1139 1071 966 761 448 295 239
106 85 33
BsmI GAATG_Cn'
Cuts at: 2407 4815 6214 6734 6827 2407
Size: 2408 1399 520 93 4803
Fragments arranged by size:
4803 2408 1399 520 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2080 2632 2820 2840 2887 3086 3259
Size: 399 290 552 188 20 47 199 173
Cuts at: 3259 3297 3330 3351 3702 3900 4469 4708 4858
Size: 38 33 21 351 198 569 239 150
Cuts at: 4858 5156 5262 5557 5618 5836 6318 6507 6542
Size: 298 106 295 61 218 482 189 35
Cuts at: 6542 7284 7369 8440 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 569 552 482 399 351
298 295 290 239 218 199 198 189
188 173 150 106 85 61 47 38
35 33 21 20
BspEI T'CCGG_A
Cuts at: 2265 8886 2265
Size: 6621 2602
BspHI T'CATG_A
Cuts at: 7112 8842 7112
Size: 1730 7493
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3291 3668 4685 6372 6578 844
Size: 2447 377 1017 1687 206 3489
Fragments arranged by size:
3489 2447 1687 1017 377 206
BsrBI CCG'CTC
Cuts at: 1394 1823 2125 3753 7110 1394
Size: 429 302 1628 3357 3507
Fragments arranged by size:
3507 3357 1628 429 302
BsrDI GCAATG_nn'
Cuts at: 65 1248 2461 7718 7892 65
Size: 1183 1213 5257 174 1396
Fragments arranged by size:
5257 1396 1213 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2031 2709 3765 4794 7867 8054 1700
Size: 110 221 678 1056 1029 3073 187 2869
Fragments arranged by size:
3073 2869 1056 1029 678 221 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9223
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9223
BssSI C'ACGA_G
Cuts at: 7283 8295 7283
Size: 1012 8211
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3292 794
Size: 2498 6725
BstBI TT'CG_AA
Cuts at: 2007 8027 2007
Size: 6020 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9223
BstXI CCAn_nnnn'nTGG
Cuts at: 2341 3460 3586 4094 2341
Size: 1119 126 508 7470
Fragments arranged by size:
7470 1119 508 126
BstYI r'GATC_y
Cuts at: 1972 3125 5290 5708 6553 6920 7305 7322 8774
Size: 1153 2165 418 845 367 385 17 1452
Cuts at: 8774 8860 8872 9218 1972
Size: 86 12 346 1977
Fragments arranged by size:
2165 1977 1452 1153 845 418 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2639 2731 5565 6369 2639
Size: 92 2834 804 5493
Fragments arranged by size:
5493 2834 804 92
BtgI C'CryG_G
Cuts at: 513 1065 2022 3633 4186 4245 4497 513
Size: 552 957 1611 553 59 252 5239
Fragments arranged by size:
5239 1611 957 553 552 252 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3230 487
Size: 683 56 2004 6480
Fragments arranged by size:
6480 2004 683 56
BtsI GCAGTG_nn'
Cuts at: 2447 3918 4699 5177 6739 7533 7553 2447
Size: 1471 781 478 1562 794 20 4117
Fragments arranged by size:
4117 1562 1471 794 781 478 20
ClaI AT'CG_AT
Cuts at: 1554 6225 1554
Size: 4671 4552
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9188
DraI TTT'AAA
Cuts at: 6527 6527
Size: 9223
DraIII CAC_nnn'GTG
Cuts at: 4125 5636 4125
Size: 1511 7712
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4969 6161 8230 809
Size: 507 3653 1192 2069 1802
Fragments arranged by size:
3653 2069 1802 1192 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2331 2604 2707
Size: 54 1425 50 275 18 501 273 103
Cuts at: 2707 3588 4084 4096 5602 5880 6670 7559 9188
Size: 881 496 12 1506 278 790 889 1629
Cuts at: 9188 8
Size: 43
Fragments arranged by size:
1629 1506 1425 889 881 790 501 496
278 275 273 103 54 50 43 18
12
EagI C'GGCC_G
Cuts at: 1537 1812 6670 1537
Size: 275 4858 4090
Fragments arranged by size:
4858 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3767 3879 3942 3986 4350 5457 5463 5469
Size: 2414 112 63 44 364 1107 6 6
Cuts at: 5469 6459 7153 1353
Size: 990 694 3423
Fragments arranged by size:
3423 2414 1107 990 694 364 112 63
44 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3784 5782 7810 8184 8330 387
Size: 1064 138 2195 1998 2028 374 146 1280
Fragments arranged by size:
2195 2028 1998 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3328 4467 4706 5154 5260 3328
Size: 1139 239 448 106 7291
Fragments arranged by size:
7291 1139 448 239 106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2363 2376 2377 2406 2528 2835 2855
Size: 393 731 13 1 29 122 307 20
Cuts at: 2855 2863 3815 4194 4453 4556 5118 5630 5811
Size: 8 952 379 259 103 562 512 181
Cuts at: 5811 6329 6461 7286 7883 8670 8938 1239
Size: 518 132 825 597 787 268 1524
Fragments arranged by size:
1524 952 825 787 731 597 562 518
512 393 379 307 268 259 181 132
122 103 29 20 13 8 1
EcoNI CCTnn'n_nnAGG
Cuts at: 3414 5826 5942 6074 6368 3414
Size: 2412 116 132 294 6269
Fragments arranged by size:
6269 2412 294 132 116
EcoO109I rG'GnC_Cy
Cuts at: 2040 2239 2698 2816 2836 3233 3741 3845 4253
Size: 199 459 118 20 397 508 104 408
Cuts at: 4253 4674 5572 5832 5833 6148 6503 2040
Size: 421 898 260 1 315 355 4760
Fragments arranged by size:
4760 898 508 459 421 408 397 355
315 260 199 118 104 20 1
EcoRI G'AATT_C
Cuts at: 6532 6910 6532
Size: 378 8845
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3865 5020 5332 6151 3865
Size: 1155 312 819 6937
Fragments arranged by size:
6937 1155 819 312
FseI GG_CCGG'CC
Cuts at: 2713 2713
Size: 9223
FspI TGC'GCA
Cuts at: 5183 7729 5183
Size: 2546 6677
HaeII r_GCGC'y
Cuts at: 1678 4492 5222 8370 1678
Size: 2814 730 3148 2531
Fragments arranged by size:
3148 2814 2531 730
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3455 3487 4130 4162 4477
Size: 32 135 32 1883 32 643 32 315
Cuts at: 4477 4509 4695 4727 5270 5302 5639 5671 7864
Size: 32 186 32 543 32 337 32 2193
Cuts at: 7864 7896 7938 7970 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2193 1883 643 543 337 315 186
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5956 6166 6567 6813 669
Size: 988 281 4018 210 401 246 3079
Fragments arranged by size:
4018 3079 988 401 281 246 210
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9223
HpaI GTT'AAC
Cuts at: 6813 6813
Size: 9223
KasI G'GCGC_C
Cuts at: 4488 5218 4488
Size: 730 8493
KpnI G_GTAC'C
Cuts at: 6548 6548
Size: 9223
MfeI C'AATT_G
Cuts at: 6822 6822
Size: 9223
MluI A'CGCG_T
Cuts at: 9099 9099
Size: 9223
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3103 3189 4284 5268 8337 8521 1305
Size: 1798 86 1095 984 3069 184 2007
Fragments arranged by size:
3069 2007 1798 1095 984 184 86
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2333 3590 4086 4098 5604
Size: 54 1425 343 501 1257 496 12 1506
Cuts at: 5604 5882 10
Size: 278 3351
Fragments arranged by size:
3351 1506 1425 1257 501 496 343 278
54 12
MslI CAynn'nnrTG
Cuts at: 518 1166 2369 3859 4653 5673 7181 7540 7699
Size: 648 1203 1490 794 1020 1508 359 159
Cuts at: 7699 518
Size: 2042
Fragments arranged by size:
2042 1508 1490 1203 1020 794 648 359
159
MspA1I CmG'CkG
Cuts at: 1606 2065 3268 3859 4473 6277 6639 7316 8464
Size: 459 1203 591 614 1804 362 677 1148
Cuts at: 8464 8709 1606
Size: 245 2120
Fragments arranged by size:
2120 1804 1203 1148 677 614 591 459
362 245
NaeI GCC'GGC
Cuts at: 2711 2711
Size: 9223
NarI GG'CG_CC
Cuts at: 4489 5219 4489
Size: 730 8493
NcoI C'CATG_G
Cuts at: 513 1065 2022 4245 4497 513
Size: 552 957 2223 252 5239
Fragments arranged by size:
5239 2223 957 552 252
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9223
NgoMIV G'CCGG_C
Cuts at: 2709 2709
Size: 9223
NheI G'CTAG_C
Cuts at: 1052 3193 1052
Size: 2141 7082
NotI GC'GGCC_GC
Cuts at: 6670 6670
Size: 9223
NsiI A_TGCA'T
Cuts at: 2376 4817 2376
Size: 2441 6782
NspI r_CATG'y
Cuts at: 1132 1229 2330 2566 3293 4172 5111 6581 8126
Size: 97 1101 236 727 879 939 1470 1545
Cuts at: 8126 1132
Size: 2229
Fragments arranged by size:
2229 1545 1470 1101 939 879 727 236
97
PasI CC'CwG_GG
Cuts at: 1348 2082 2684 1348
Size: 734 602 7887
Fragments arranged by size:
7887 734 602
PciI A'CATG_T
Cuts at: 2562 8122 2562
Size: 5560 3663
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2222 4498 5965 1108
Size: 1114 2276 1467 4366
Fragments arranged by size:
4366 2276 1467 1114
PfoI T'CCnGG_A
Cuts at: 3549 3549
Size: 9223
PmeI GTTT'AAAC
Cuts at: 6527 6527
Size: 9223
PmlI CAC'GTG
Cuts at: 6319 6319
Size: 9223
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5611 5643 7292 7324 8805 8837 5611
Size: 32 1649 32 1481 32 5997
Fragments arranged by size:
5997 1649 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2040 3233 3741 3845 4674 5572 6148 2040
Size: 1193 508 104 829 898 576 5115
Fragments arranged by size:
5115 1193 898 829 576 508 104
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9223
PsiI TTA'TAA
Cuts at: 6793 6793
Size: 9223
PspOMI G'GGCC_C
Cuts at: 2816 2836 5832 6503 2816
Size: 20 2996 671 5536
Fragments arranged by size:
5536 2996 671 20
PspXI vC'TCGA_Gb
Cuts at: 4859 4859
Size: 9223
PstI C_TGCA'G
Cuts at: 830 3190 3288 4694 6575 830
Size: 2360 98 1406 1881 3478
Fragments arranged by size:
3478 2360 1881 1406 98
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9223
PvuII CAG'CTG
Cuts at: 2065 3268 3859 4473 6277 2065
Size: 1203 591 614 1804 5011
Fragments arranged by size:
5011 1804 1203 614 591
SacI G_AGCT'C
Cuts at: 3330 4469 4708 5156 5262 3330
Size: 1139 239 448 106 7291
Fragments arranged by size:
7291 1139 448 239 106
SalI G'TCGA_C
Cuts at: 1936 6565 1936
Size: 4629 4594
SanDI GG'GwC_CC
Cuts at: 3741 3845 3741
Size: 104 9119
SapI GCTCTTCn'nnn_
Cuts at: 3986 6459 3986
Size: 2473 6750
SbfI CC_TGCA'GG
Cuts at: 6575 6575
Size: 9223
ScaI AGT'ACT
Cuts at: 7471 9183 7471
Size: 1712 7511
SexAI A'CCwGG_T
Cuts at: 4121 4121
Size: 9223
SfcI C'TryA_G
Cuts at: 826 1045 2590 2661 3186 3284 3411 4440 4474
Size: 219 1545 71 525 98 127 1029 34
Cuts at: 4474 4690 6571 7706 8387 8578 826
Size: 216 1881 1135 681 191 1471
Fragments arranged by size:
1881 1545 1471 1135 1029 681 525 219
216 191 127 98 71 34
SfoI GGC'GCC
Cuts at: 4490 5220 4490
Size: 730 8493
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9223
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9223
SmaI CCC'GGG
Cuts at: 1872 3746 5234 6200 6550 1872
Size: 1874 1488 966 350 4545
Fragments arranged by size:
4545 1874 1488 966 350
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2096 2273 4299 4859 5036 5617
Size: 197 926 153 177 2026 560 177 581
Cuts at: 5617 7327 8228 8490 820
Size: 1710 901 262 1553
Fragments arranged by size:
2026 1710 1553 926 901 581 560 262
197 177 177 153
SnaBI TAC'GTA
Cuts at: 493 6520 7014 493
Size: 6027 494 2702
Fragments arranged by size:
6027 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9223
SphI G_CATG'C
Cuts at: 1132 6581 1132
Size: 5449 3774
SspI AAT'ATT
Cuts at: 5 52 5872 7147 5
Size: 47 5820 1275 2081
Fragments arranged by size:
5820 2081 1275 47
StuI AGG'CCT
Cuts at: 1517 2876 3107 4093 5315 1517
Size: 1359 231 986 1222 5425
Fragments arranged by size:
5425 1359 1222 986 231
StyI C'CwwG_G
Cuts at: 513 1065 2022 3702 4245 4497 5092 5883 5938
Size: 552 957 1680 543 252 595 791 55
Cuts at: 5938 6070 6300 6662 513
Size: 132 230 362 3074
Fragments arranged by size:
3074 1680 957 791 595 552 543 362
252 230 132 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3710 5190 7250 7267 7420 7605 8050
Size: 1255 2130 1480 2060 17 153 185 445
Cuts at: 8050 325
Size: 1498
Fragments arranged by size:
2130 2060 1498 1480 1255 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5355 7469 9181
Size: 274 80 33 51 1443 3378 2114 1712
Cuts at: 9181 96
Size: 138
Fragments arranged by size:
3378 2114 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5622 5654 9183 9215 5622
Size: 32 3529 32 5630
Fragments arranged by size:
5630 3529 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3361 5284 1316
Size: 2045 1923 5255
Fragments arranged by size:
5255 2045 1923
XbaI T'CTAG_A
Cuts at: 4618 6559 4618
Size: 1941 7282
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2494 2894 6140 2494
Size: 400 3246 5577
Fragments arranged by size:
5577 3246 400
XhoI C'TCGA_G
Cuts at: 1943 4859 5617 1943
Size: 2916 758 5549
Fragments arranged by size:
5549 2916 758
XmaI C'CCGG_G
Cuts at: 1870 3744 5232 6198 6548 1870
Size: 1874 1488 966 350 4545
Fragments arranged by size:
4545 1874 1488 966 350
XmnI GAAnn'nnTTC
Cuts at: 6010 7352 6010
Size: 1342 7881
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7031 276
Size: 53 83 186 936 5497 2468
Fragments arranged by size:
5497 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BsiWI BsmBI BstZ17I EcoRV
FspAI NruI PacI PsrI RsrII SacII SfiI SrfI
SwaI