Restriction map for
pFN21ASDA1197
(Circular) (Six-base) MAPSORT of: pFN21ASDA1197.seq Check: 5736 from: 1 to: 8995
pFN21ASDA1197 8995 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3535 5287 3535
Size: 1752 7243
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6805 278
Size: 53 83 186 936 5269 2468
Fragments arranged by size:
5269 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6338 1937
Size: 4401 4594
Acc65I G'GTAC_C
Cuts at: 3979 6316 3979
Size: 2337 6658
AclI AA'CG_TT
Cuts at: 1563 4238 7122 7495 1563
Size: 2675 2884 373 3063
Fragments arranged by size:
3063 2884 2675 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2963 3210 4311 4959 6125 7058 8442 8710
Size: 1331 247 1101 648 1166 933 1384 268
Cuts at: 8710 1632
Size: 1917
Fragments arranged by size:
1917 1384 1331 1166 1101 933 648 268
247
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8798
AflIII A'CryG_T
Cuts at: 1203 2324 6072 7894 8871 1203
Size: 1121 3748 1822 977 1327
Fragments arranged by size:
3748 1822 1327 1121 977
AgeI A'CCGG_T
Cuts at: 7826 7826
Size: 8995
AhdI GACnn_n'nnGTC
Cuts at: 7724 7724
Size: 8995
AleI CACnn'nnGTG
Cuts at: 1166 3785 1166
Size: 2619 6376
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3337 3507 1184
Size: 2153 170 6672
Fragments arranged by size:
6672 2153 170
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5963 5995 5963
Size: 32 8963
AlwNI CAG_nnn'CTG
Cuts at: 2224 3839 4045 4561 5336 6005 6738 8310 2224
Size: 1615 206 516 775 669 733 1572 2909
Fragments arranged by size:
2909 1615 1572 775 733 669 516 206
ApaLI G'TGCA_C
Cuts at: 7052 8208 7052
Size: 1156 7839
ApoI r'AATT_y
Cuts at: 1493 2116 2363 3642 4276 6008 6304 6530 6682
Size: 623 247 1279 634 1732 296 226 152
Cuts at: 6682 8791 1493
Size: 2109 1697
Fragments arranged by size:
2109 1732 1697 1279 634 623 296 247
226 152
AseI AT'TA_AT
Cuts at: 160 1032 2763 3507 5510 7549 160
Size: 872 1731 744 2003 2039 1606
Fragments arranged by size:
2039 2003 1731 1606 872 744
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8995
AvaI C'yCGr_G
Cuts at: 1870 1943 2124 5804 5885 6320 1870
Size: 73 181 3680 81 435 4545
Fragments arranged by size:
4545 3680 435 181 81 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2322 2355 3147 3180 2322
Size: 33 792 33 8137
Fragments arranged by size:
8137 792 33 33
BamHI G'GATC_C
Cuts at: 4214 6325 6692 4214
Size: 2111 367 6517
Fragments arranged by size:
6517 2111 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3979 4427 5709 6316 7771
Size: 325 217 627 2192 448 1282 607 1455
Cuts at: 7771 618
Size: 1842
Fragments arranged by size:
2192 1842 1455 1282 627 607 448 325
217
BanII G_rGCy'C
Cuts at: 1391 5805 6314 1391
Size: 4414 509 4072
Fragments arranged by size:
4414 4072 509
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2288 3036 4817 4950 5357 928
Size: 971 389 748 1781 133 407 4566
Fragments arranged by size:
4566 1781 971 748 407 389 133
BbvCI CC'TCA_GC
Cuts at: 5912 5912
Size: 8995
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5215 5249 7186 7220 1903
Size: 34 3278 34 1937 34 3678
Fragments arranged by size:
3678 3278 1937 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2554 5125 6152 6887 8097 2554
Size: 2571 1027 735 1210 3452
Fragments arranged by size:
3452 2571 1210 1027 735
BclI T'GATC_A
Cuts at: 1548 3789 1548
Size: 2241 6754
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4271 7606 136
Size: 107 122 71 631 605 2599 3335 1525
Fragments arranged by size:
3335 2599 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3591 8990 3591
Size: 5399 3596
BlpI GC'TnA_GC
Cuts at: 4155 4405 6412 4155
Size: 250 2007 6738
Fragments arranged by size:
6738 2007 250
Bme1580I G_kGCm'C
Cuts at: 1790 3828 4604 5813 7056 8212 1790
Size: 2038 776 1209 1243 1156 2573
Fragments arranged by size:
2573 2038 1243 1209 1156 776
BmgBI CAC'GTC
Cuts at: 1318 5070 5779 1318
Size: 3752 709 4534
Fragments arranged by size:
4534 3752 709
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3101 3561 3937
Size: 416 53 83 399 24 1684 460 376
Cuts at: 3937 4665 4688 6726 7684 442
Size: 728 23 2038 958 1753
Fragments arranged by size:
2038 1753 1684 958 728 460 416 399
376 83 53 24 23
BmtI G_CTAG'C
Cuts at: 1056 3186 1056
Size: 2130 6865
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3453 3948 4379 4563 5122 5232 5292 5313
Size: 2214 495 431 184 559 110 60 21
Cuts at: 5313 5669 5873 7655 1239
Size: 356 204 1782 2579
Fragments arranged by size:
2579 2214 1782 559 495 431 356 204
184 110 60 21
Bpu10I CC'TnA_GC
Cuts at: 5912 5912
Size: 8995
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4341 4568 7120 7985 8283 4341
Size: 227 2552 865 298 5053
Fragments arranged by size:
5053 2552 865 298 227
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2530 3616 7658 882
Size: 617 1031 1086 4042 2219
Fragments arranged by size:
4042 2219 1086 1031 617
BsaAI yAC'GTr
Cuts at: 493 1204 3478 3823 6292 6786 493
Size: 711 2274 345 2469 494 2702
Fragments arranged by size:
2702 2469 2274 711 494 345
BsaBI GATnn'nnATC
Cuts at: 2927 5939 6038 8989 2927
Size: 3012 99 2951 2933
Fragments arranged by size:
3012 2951 2933 99
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6802 7184 7819 275
Size: 53 83 186 936 5269 382 635 1451
Fragments arranged by size:
5269 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2683 5117 5734 7428 7826 8100 8247 8658 2683
Size: 2434 617 1694 398 274 147 411 3020
Fragments arranged by size:
3020 2434 1694 617 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3451 3481 3578 3608 1178
Size: 30 165 30 2048 30 97 30 6565
Fragments arranged by size:
6565 2048 165 97 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4449 4452 4455 4458 4461 4464 4467 4470
Size: 3263 3 3 3 3 3 3 3
Cuts at: 4470 4509 4512 4515 4530 5815 5909 5912 1186
Size: 39 3 3 15 1285 94 3 4269
Fragments arranged by size:
4269 3263 1285 94 39 15 3 3
3 3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2756 2865 3178 5177 5318 5431 8198 8694 2756
Size: 109 313 1999 141 113 2767 496 3057
Fragments arranged by size:
3057 2767 1999 496 313 141 113 109
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2159 4184 4683 4911 5240 1163
Size: 573 423 2025 499 228 329 4918
Fragments arranged by size:
4918 2025 573 499 423 329 228
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6445 7206 8234 1540
Size: 275 186 4444 761 1028 2301
Fragments arranged by size:
4444 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 7056 7141 8212 7056
Size: 85 1071 7839
Fragments arranged by size:
7839 1071 85
BsmI GAATG_Cn'
Cuts at: 2608 6506 6599 2608
Size: 3898 93 5004
Fragments arranged by size:
5004 3898 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2981 2981
Size: 8995
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3828 4604 5805 5813 6314 7056 7141
Size: 399 2038 776 1201 8 501 742 85
Cuts at: 7141 8212 1391
Size: 1071 2174
Fragments arranged by size:
2174 2038 1201 1071 776 742 501 399
85 8
BspEI T'CCGG_A
Cuts at: 2683 5117 8658 2683
Size: 2434 3541 3020
Fragments arranged by size:
3541 3020 2434
BspHI T'CATG_A
Cuts at: 6884 8614 6884
Size: 1730 7265
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2282 3535 5042 5287 5868 5994 6350 844
Size: 1438 1253 1507 245 581 126 356 3489
Fragments arranged by size:
3489 1507 1438 1253 581 356 245 126
BsrBI CCG'CTC
Cuts at: 1394 1823 6882 1394
Size: 429 5059 3507
Fragments arranged by size:
5059 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2668 3443 5622 7490 7664 65
Size: 1183 1420 775 2179 1868 174 1396
Fragments arranged by size:
2179 1868 1420 1396 1183 775 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3494 7639 7826 1700
Size: 110 1684 4145 187 2869
Fragments arranged by size:
4145 2869 1684 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8995
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8995
BssSI C'ACGA_G
Cuts at: 5075 7055 8067 5075
Size: 1980 1012 6003
Fragments arranged by size:
6003 1980 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5339 794
Size: 4545 4450
BstBI TT'CG_AA
Cuts at: 2007 7799 2007
Size: 5792 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8995
BstXI CCAn_nnnn'nTGG
Cuts at: 4261 4637 4261
Size: 376 8619
BstYI r'GATC_y
Cuts at: 1972 3591 4041 4214 6325 6692 7077 7094 8546
Size: 1619 450 173 2111 367 385 17 1452
Cuts at: 8546 8632 8644 8990 1972
Size: 86 12 346 1977
Fragments arranged by size:
2111 1977 1619 1452 450 385 367 346
173 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3735 5750 3735
Size: 2015 6980
BtgI C'CryG_G
Cuts at: 513 1065 3286 3597 4765 4902 5025 6054 6084
Size: 552 2221 311 1168 137 123 1029 30
Cuts at: 6084 513
Size: 3424
Fragments arranged by size:
3424 2221 1168 1029 552 311 137 123
30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2996 5683 487
Size: 683 56 1770 2687 3799
Fragments arranged by size:
3799 2687 1770 683 56
BtsI GCAGTG_nn'
Cuts at: 3489 4471 5412 5514 6511 7305 7325 3489
Size: 982 941 102 997 794 20 5159
Fragments arranged by size:
5159 997 982 941 794 102 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8995
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2637 2672 5538 5573 529
Size: 35 2073 35 2866 35 3951
Fragments arranged by size:
3951 2866 2073 35 35 35
DraI TTT'AAA
Cuts at: 6299 6299
Size: 8995
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8002 809
Size: 507 6686 1802
Fragments arranged by size:
6686 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4036 4263 4615
Size: 54 1425 50 275 18 2206 227 352
Cuts at: 4615 6442 7331 8960 8
Size: 1827 889 1629 43
Fragments arranged by size:
2206 1827 1629 1425 889 352 275 227
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6442 1537
Size: 275 4630 4090
Fragments arranged by size:
4630 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2854 4069 4202 5952 6925 1353
Size: 1501 1215 133 1750 973 3423
Fragments arranged by size:
3423 1750 1501 1215 973 133
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7582 7956 8102 387
Size: 1064 138 5993 374 146 1280
Fragments arranged by size:
5993 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2963 3210 3453 3948 4311 4379 4563
Size: 393 1331 247 243 495 363 68 184
Cuts at: 4563 4959 5122 5232 5292 5313 5669 5873 6125
Size: 396 163 110 60 21 356 204 252
Cuts at: 6125 7058 7655 8442 8710 1239
Size: 933 597 787 268 1524
Fragments arranged by size:
1524 1331 933 787 597 495 396 393
363 356 268 252 247 243 204 184
163 110 68 60 21
EcoNI CCTnn'n_nnAGG
Cuts at: 3759 3930 5387 6140 3759
Size: 171 1457 753 6614
Fragments arranged by size:
6614 1457 753 171
EcoO109I rG'GnC_Cy
Cuts at: 2742 5857 2742
Size: 3115 5880
EcoRI G'AATT_C
Cuts at: 2116 6304 6682 2116
Size: 4188 378 4429
Fragments arranged by size:
4429 4188 378
EcoRV GAT'ATC
Cuts at: 2929 4551 6133 2929
Size: 1622 1582 5791
Fragments arranged by size:
5791 1622 1582
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2463 2580 2463
Size: 117 8878
FspI TGC'GCA
Cuts at: 7501 7501
Size: 8995
HaeII r_GCGC'y
Cuts at: 1678 5067 8142 1678
Size: 3389 3075 2531
Fragments arranged by size:
3389 3075 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2060 2092 2435 2456 2467
Size: 32 135 32 488 32 343 21 11
Cuts at: 2467 2488 3451 3483 4863 4895 5750 5782 5798
Size: 21 963 32 1380 32 855 32 16
Cuts at: 5798 5830 6157 6189 7636 7668 7710 7742 1373
Size: 32 327 32 1447 32 42 32 2626
Fragments arranged by size:
2626 1447 1380 963 855 488 343 327
135 42 32 32 32 32 32 32
32 32 32 32 21 21 16 11
HincII GTy'rAC
Cuts at: 669 1657 1938 2712 6339 6585 669
Size: 988 281 774 3627 246 3079
Fragments arranged by size:
3627 3079 988 774 281 246
HindIII A'AGCT_T
Cuts at: 748 3114 748
Size: 2366 6629
HpaI GTT'AAC
Cuts at: 6585 6585
Size: 8995
KpnI G_GTAC'C
Cuts at: 3983 6320 3983
Size: 2337 6658
MfeI C'AATT_G
Cuts at: 2109 3720 4122 6594 2109
Size: 1611 402 2472 4510
Fragments arranged by size:
4510 2472 1611 402
MluI A'CGCG_T
Cuts at: 8871 8871
Size: 8995
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6136 8109 8293 1305
Size: 4831 1973 184 2007
Fragments arranged by size:
4831 2007 1973 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4038 4265 4617 10
Size: 54 1425 343 2206 227 352 4388
Fragments arranged by size:
4388 2206 1425 352 343 227 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3251 3389 3785 6953 7312 7471 518
Size: 648 2085 138 396 3168 359 159 2042
Fragments arranged by size:
3168 2085 2042 648 396 359 159 138
MspA1I CmG'CkG
Cuts at: 1606 2599 4196 4409 4473 5336 5538 5600 5715
Size: 993 1597 213 64 863 202 62 115
Cuts at: 5715 6121 6411 7088 8236 8481 1606
Size: 406 290 677 1148 245 2120
Fragments arranged by size:
2120 1597 1148 993 863 677 406 290
245 213 202 115 64 62
NaeI GCC'GGC
Cuts at: 3496 3496
Size: 8995
NcoI C'CATG_G
Cuts at: 513 1065 3286 4765 513
Size: 552 2221 1479 4743
Fragments arranged by size:
4743 2221 1479 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8995
NgoMIV G'CCGG_C
Cuts at: 3494 3494
Size: 8995
NheI G'CTAG_C
Cuts at: 1052 3182 1052
Size: 2130 6865
NotI GC'GGCC_GC
Cuts at: 6442 6442
Size: 8995
NspI r_CATG'y
Cuts at: 1132 1229 2328 6353 7898 1132
Size: 97 1099 4025 1545 2229
Fragments arranged by size:
4025 2229 1545 1099 97
PasI CC'CwG_GG
Cuts at: 1348 4219 4597 5999 1348
Size: 2871 378 1402 4344
Fragments arranged by size:
4344 2871 1402 378
PciI A'CATG_T
Cuts at: 2324 7894 2324
Size: 5570 3425
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4045 1108
Size: 2937 6058
PfoI T'CCnGG_A
Cuts at: 3925 5646 3925
Size: 1721 7274
PmeI GTTT'AAAC
Cuts at: 6299 6299
Size: 8995
PmlI CAC'GTG
Cuts at: 3478 3823 3478
Size: 345 8650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2877 2909 7064 7096 8577 8609 2877
Size: 32 4155 32 1481 32 3263
Fragments arranged by size:
4155 3263 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2742 5857 2742
Size: 3115 5880
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8995
PsiI TTA'TAA
Cuts at: 6565 6565
Size: 8995
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4513 4545 4513
Size: 32 8963
PstI C_TGCA'G
Cuts at: 830 2293 5491 6051 6347 830
Size: 1463 3198 560 296 3478
Fragments arranged by size:
3478 3198 1463 560 296
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8995
PvuII CAG'CTG
Cuts at: 4409 5336 5538 6121 4409
Size: 927 202 583 7283
Fragments arranged by size:
7283 927 583 202
SalI G'TCGA_C
Cuts at: 1936 6337 1936
Size: 4401 4594
SanDI GG'GwC_CC
Cuts at: 2742 2742
Size: 8995
SapI GCTCTTCn'nnn_
Cuts at: 4069 4069
Size: 8995
SbfI CC_TGCA'GG
Cuts at: 6347 6347
Size: 8995
ScaI AGT'ACT
Cuts at: 4531 5567 7243 8955 4531
Size: 1036 1676 1712 4571
Fragments arranged by size:
4571 1712 1676 1036
SexAI A'CCwGG_T
Cuts at: 3805 3805
Size: 8995
SfcI C'TryA_G
Cuts at: 826 1045 2289 5292 5487 6047 6111 6343 7478
Size: 219 1244 3003 195 560 64 232 1135
Cuts at: 7478 8159 8350 826
Size: 681 191 1471
Fragments arranged by size:
3003 1471 1244 1135 681 560 232 219
195 191 64
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8995
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8995
SmaI CCC'GGG
Cuts at: 1872 6322 1872
Size: 4450 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2124 4356 4583 7099 8000 8262
Size: 197 926 181 2232 227 2516 901 262
Cuts at: 8262 820
Size: 1553
Fragments arranged by size:
2516 2232 1553 926 901 262 227 197
181
SnaBI TAC'GTA
Cuts at: 493 6292 6786 493
Size: 5799 494 2702
Fragments arranged by size:
5799 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8995
SphI G_CATG'C
Cuts at: 1132 6353 1132
Size: 5221 3774
SspI AAT'ATT
Cuts at: 5 52 6211 6273 6919 5
Size: 47 6159 62 646 2081
Fragments arranged by size:
6159 2081 646 62 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8995
StyI C'CwwG_G
Cuts at: 513 1065 3286 3616 3966 4053 4105 4176 4669
Size: 552 2221 330 350 87 52 71 493
Cuts at: 4669 4765 6434 513
Size: 96 1669 3074
Fragments arranged by size:
3074 2221 1669 552 493 350 330 96
87 71 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3259 5389 7022 7039 7192 7377 7822
Size: 1255 1679 2130 1633 17 153 185 445
Cuts at: 7822 325
Size: 1498
Fragments arranged by size:
2130 1679 1633 1498 1255 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3569 4529 5565
Size: 274 80 33 51 1443 1592 960 1036
Cuts at: 5565 7241 8953 96
Size: 1676 1712 138
Fragments arranged by size:
1712 1676 1592 1443 1036 960 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8955 8987 8955
Size: 32 8963
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8995
XbaI T'CTAG_A
Cuts at: 2567 6331 2567
Size: 3764 5231
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4660 4660
Size: 8995
XhoI C'TCGA_G
Cuts at: 1943 2124 1943
Size: 181 8814
XmaI C'CCGG_G
Cuts at: 1870 6320 1870
Size: 4450 4545
XmnI GAAnn'nnTTC
Cuts at: 3173 3625 7124 3173
Size: 452 3499 5044
Fragments arranged by size:
5044 3499 452
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6803 276
Size: 53 83 186 936 5269 2468
Fragments arranged by size:
5269 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BfrBI BplI BsiWI
BstZ17I DraIII EcoICRI FseI FspAI KasI NarI NruI
NsiI PacI PspOMI PspXI RsrII SacI SacII SfiI
SfoI SrfI SwaI