Restriction map for
pFN21ASDA1195
(Circular) (Six-base) MAPSORT of: pFN21ASDA1195.seq Check: 3206 from: 1 to: 6856
pFN21ASDA1195 6856 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2148 2269 2283 2148
Size: 121 14 6721
Fragments arranged by size:
6721 121 14
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4666 278
Size: 53 83 186 936 3130 2468
Fragments arranged by size:
3130 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4199 1937
Size: 2262 4594
Acc65I G'GTAC_C
Cuts at: 2563 4177 2563
Size: 1614 5242
AclI AA'CG_TT
Cuts at: 1563 4983 5356 1563
Size: 3420 373 3063
Fragments arranged by size:
3420 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2419 3147 3454 4034 4919 6303 6571 1632
Size: 787 728 307 580 885 1384 268 1917
Fragments arranged by size:
1917 1384 885 787 728 580 307 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6659
AflIII A'CryG_T
Cuts at: 1203 2163 2946 5755 6732 1203
Size: 960 783 2809 977 1327
Fragments arranged by size:
2809 1327 977 960 783
AgeI A'CCGG_T
Cuts at: 5687 5687
Size: 6856
AhdI GACnn_n'nnGTC
Cuts at: 3520 5585 3520
Size: 2065 4791
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6856
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6856
AlwNI CAG_nnn'CTG
Cuts at: 2708 4599 6171 2708
Size: 1891 1572 3393
Fragments arranged by size:
3393 1891 1572
ApaLI G'TGCA_C
Cuts at: 2957 4913 6069 2957
Size: 1956 1156 3744
Fragments arranged by size:
3744 1956 1156
ApoI r'AATT_y
Cuts at: 1493 2719 3464 3733 4165 4391 4543 6652 1493
Size: 1226 745 269 432 226 152 2109 1697
Fragments arranged by size:
2109 1697 1226 745 432 269 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5410 160
Size: 872 4378 1606
Fragments arranged by size:
4378 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6856
AvaI C'yCGr_G
Cuts at: 1870 1943 4095 4181 1870
Size: 73 2152 86 4545
Fragments arranged by size:
4545 2152 86 73
BamHI G'GATC_C
Cuts at: 4186 4553 4186
Size: 367 6489
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2052 2150 2563 3911 4177
Size: 325 217 627 265 98 413 1348 266
Cuts at: 4177 5632 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1348 627 413 325 266 265
217 98
BanII G_rGCy'C
Cuts at: 1391 3106 3399 3642 4175 1391
Size: 1715 293 243 533 4072
Fragments arranged by size:
4072 1715 533 293 243
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5885
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2837 2871 5047 5081 1903
Size: 34 900 34 2176 34 3678
Fragments arranged by size:
3678 2176 900 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3151 4748 5958 3151
Size: 1597 1210 4049
Fragments arranged by size:
4049 1597 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6856
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5467 136
Size: 107 122 71 631 605 3795 1525
Fragments arranged by size:
3795 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3202 3936 6851 3202
Size: 734 2915 3207
Fragments arranged by size:
3207 2915 734
BlpI GC'TnA_GC
Cuts at: 3680 4273 3680
Size: 593 6263
Bme1580I G_kGCm'C
Cuts at: 1790 2055 2288 2961 4917 6073 1790
Size: 265 233 673 1956 1156 2573
Fragments arranged by size:
2573 1956 1156 673 265 233
BmgBI CAC'GTC
Cuts at: 1318 3256 1318
Size: 1938 4918
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2376 4587 5545
Size: 416 53 83 399 24 959 2211 958
Cuts at: 5545 442
Size: 1753
Fragments arranged by size:
2211 1753 959 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2633 1056
Size: 1577 5279
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3059 3206 3657 3776 3940 5516 1239
Size: 1820 147 451 119 164 1576 2579
Fragments arranged by size:
2579 1820 1576 451 164 147 119
Bpu10I CC'TnA_GC
Cuts at: 3045 3045
Size: 6856
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3626 4981 5846 6144 3626
Size: 1355 865 298 4338
Fragments arranged by size:
4338 1355 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2369 5519 882
Size: 617 870 3150 2219
Fragments arranged by size:
3150 2219 870 617
BsaAI yAC'GTr
Cuts at: 493 1204 3391 4153 4647 493
Size: 711 2187 762 494 2702
Fragments arranged by size:
2702 2187 762 711 494
BsaBI GATnn'nnATC
Cuts at: 6850 6850
Size: 6856
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4663 5045 5680 275
Size: 53 83 186 936 3130 382 635 1451
Fragments arranged by size:
3130 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5289 5687 5961 6108 6519 5289
Size: 398 274 147 411 5626
Fragments arranged by size:
5626 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2523 2553 1178
Size: 30 165 30 1120 30 5481
Fragments arranged by size:
5481 1120 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2180 2402 2501 2547 1186
Size: 994 222 99 46 5495
Fragments arranged by size:
5495 994 222 99 46
BseYI C'CCAG_C
Cuts at: 3776 3804 3975 6059 6555 3776
Size: 28 171 2084 496 4077
Fragments arranged by size:
4077 2084 496 171 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3476 1163
Size: 573 1740 4543
Fragments arranged by size:
4543 1740 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4306 5067 6095 1540
Size: 275 186 2305 761 1028 2301
Fragments arranged by size:
2305 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2184 2700 2961 3106 3399 3642 4011 4044 4917
Size: 516 261 145 293 243 369 33 873
Cuts at: 4917 5002 6073 2184
Size: 85 1071 2967
Fragments arranged by size:
2967 1071 873 516 369 293 261 243
145 85 33
BsmI GAATG_Cn'
Cuts at: 4367 4460 4367
Size: 93 6763
BsmBI CGTCTCn'nnnn_
Cuts at: 4150 4150
Size: 6856
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2184 2288 2700 2961 3106 3399
Size: 399 265 129 104 412 261 145 293
Cuts at: 3399 3642 4011 4044 4175 4917 5002 6073 1391
Size: 243 369 33 131 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 412 399 369 293 265
261 243 145 131 129 104 85 33
BspEI T'CCGG_A
Cuts at: 6519 6519
Size: 6856
BspHI T'CATG_A
Cuts at: 2356 2367 4745 6475 2356
Size: 11 2378 1730 2737
Fragments arranged by size:
2737 2378 1730 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2148 2197 2269 2283 3198 4211 844
Size: 1304 49 72 14 915 1013 3489
Fragments arranged by size:
3489 1304 1013 915 72 49 14
BsrBI CCG'CTC
Cuts at: 1394 1823 4743 1394
Size: 429 2920 3507
Fragments arranged by size:
3507 2920 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3771 3887 5351 5525 65
Size: 1183 2523 116 1464 174 1396
Fragments arranged by size:
2523 1464 1396 1183 174 116
BsrFI r'CCGG_y
Cuts at: 1700 1810 5500 5687 1700
Size: 110 3690 187 2869
Fragments arranged by size:
3690 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3002 3575 96
Size: 2906 573 3377
Fragments arranged by size:
3377 2906 573
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6856
BssSI C'ACGA_G
Cuts at: 4131 4916 5928 4131
Size: 785 1012 5059
Fragments arranged by size:
5059 1012 785
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2708 794
Size: 1914 4942
BstBI TT'CG_AA
Cuts at: 2007 5660 2007
Size: 3653 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6856
BstYI r'GATC_y
Cuts at: 1972 2039 2438 3202 3936 4186 4553 4938 4955
Size: 67 399 764 734 250 367 385 17
Cuts at: 4955 6407 6493 6505 6851 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 764 734 399 385 367 346
250 86 67 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2310 2865 3904 513
Size: 552 957 288 555 1039 3465
Fragments arranged by size:
3465 1039 957 555 552 288
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6117
Fragments arranged by size:
6117 683 56
BtsI GCAGTG_nn'
Cuts at: 2086 2544 3159 4372 5166 5186 2086
Size: 458 615 1213 794 20 3756
Fragments arranged by size:
3756 1213 794 615 458 20
ClaI AT'CG_AT
Cuts at: 1554 3550 1554
Size: 1996 4860
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2045 2080 2863 2898 529
Size: 35 1481 35 783 35 4487
Fragments arranged by size:
4487 1481 783 35 35 35
DraI TTT'AAA
Cuts at: 4160 4160
Size: 6856
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5863 809
Size: 507 4547 1802
Fragments arranged by size:
4547 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2265 2327 2668
Size: 54 1425 50 275 18 435 62 341
Cuts at: 2668 2782 4303 5192 6821 8
Size: 114 1521 889 1629 43
Fragments arranged by size:
1629 1521 1425 889 435 341 275 114
62 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4303 1537
Size: 275 2491 4090
Fragments arranged by size:
4090 2491 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2438 3962 4786 1353
Size: 1085 1524 824 3423
Fragments arranged by size:
3423 1524 1085 824
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2983 5443 5817 5963 387
Size: 1064 138 1394 2460 374 146 1280
Fragments arranged by size:
2460 1394 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3104 3397 3640 3104
Size: 293 243 6320
Fragments arranged by size:
6320 293 243
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2419 3059 3147 3206 3454 3657 3776
Size: 393 787 640 88 59 248 203 119
Cuts at: 3776 3940 4034 4919 5516 6303 6571 1239
Size: 164 94 885 597 787 268 1524
Fragments arranged by size:
1524 885 787 787 640 597 393 268
248 203 164 119 94 88 59
EcoO109I rG'GnC_Cy
Cuts at: 2428 3187 2428
Size: 759 6097
EcoRI G'AATT_C
Cuts at: 3733 4165 4543 3733
Size: 432 378 6046
Fragments arranged by size:
6046 432 378
EcoRV GAT'ATC
Cuts at: 2554 2554
Size: 6856
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2449 2449
Size: 6856
FspI TGC'GCA
Cuts at: 5362 5362
Size: 6856
HaeII r_GCGC'y
Cuts at: 1678 2811 6003 1678
Size: 1133 3192 2531
Fragments arranged by size:
3192 2531 1133
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2538 2570 3506 3538 3543
Size: 32 135 32 966 32 936 32 5
Cuts at: 3543 3575 3855 3887 5497 5529 5571 5603 1373
Size: 32 280 32 1610 32 42 32 2626
Fragments arranged by size:
2626 1610 966 936 280 135 42 32
32 32 32 32 32 32 32 5
HincII GTy'rAC
Cuts at: 669 1657 1938 4200 4446 669
Size: 988 281 2262 246 3079
Fragments arranged by size:
3079 2262 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6856
HpaI GTT'AAC
Cuts at: 4446 4446
Size: 6856
KpnI G_GTAC'C
Cuts at: 2567 4181 2567
Size: 1614 5242
MfeI C'AATT_G
Cuts at: 4455 4455
Size: 6856
MluI A'CGCG_T
Cuts at: 6732 6732
Size: 6856
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5970 6154 1305
Size: 4665 184 2007
Fragments arranged by size:
4665 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2267 2329 2670 2784 10
Size: 54 1425 343 435 62 341 114 4082
Fragments arranged by size:
4082 1425 435 343 341 114 62 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2099 3998 4814 5173 5332 518
Size: 648 933 1899 816 359 159 2042
Fragments arranged by size:
2042 1899 933 816 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2325 2924 3368 4272 4949 6097 6342 1606
Size: 719 599 444 904 677 1148 245 2120
Fragments arranged by size:
2120 1148 904 719 677 599 444 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 2310 2865 3904 513
Size: 552 957 288 555 1039 3465
Fragments arranged by size:
3465 1039 957 555 552 288
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6856
NheI G'CTAG_C
Cuts at: 1052 2629 1052
Size: 1577 5279
NotI GC'GGCC_GC
Cuts at: 4303 4303
Size: 6856
NspI r_CATG'y
Cuts at: 1132 1229 2167 2950 4075 4214 5759 1132
Size: 97 938 783 1125 139 1545 2229
Fragments arranged by size:
2229 1545 1125 938 783 139 97
PasI CC'CwG_GG
Cuts at: 1348 3377 3605 1348
Size: 2029 228 4599
Fragments arranged by size:
4599 2029 228
PciI A'CATG_T
Cuts at: 2163 2946 5755 2163
Size: 783 2809 3264
Fragments arranged by size:
3264 2809 783
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2263 2316 2791 3709 1108
Size: 1155 53 475 918 4255
Fragments arranged by size:
4255 1155 918 475 53
PfoI T'CCnGG_A
Cuts at: 3917 3917
Size: 6856
PmeI GTTT'AAAC
Cuts at: 4160 4160
Size: 6856
PmlI CAC'GTG
Cuts at: 3391 3391
Size: 6856
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4925 4957 6438 6470 4925
Size: 32 1481 32 5311
Fragments arranged by size:
5311 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2428 3187 2428
Size: 759 6097
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6856
PsiI TTA'TAA
Cuts at: 4426 4426
Size: 6856
PstI C_TGCA'G
Cuts at: 830 2280 2418 4208 830
Size: 1450 138 1790 3478
Fragments arranged by size:
3478 1790 1450 138
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6856
PvuII CAG'CTG
Cuts at: 2924 2924
Size: 6856
SacI G_AGCT'C
Cuts at: 3106 3399 3642 3106
Size: 293 243 6320
Fragments arranged by size:
6320 293 243
SalI G'TCGA_C
Cuts at: 1936 4198 1936
Size: 2262 4594
SbfI CC_TGCA'GG
Cuts at: 2280 2418 4208 2280
Size: 138 1790 4928
Fragments arranged by size:
4928 1790 138
ScaI AGT'ACT
Cuts at: 5104 6816 5104
Size: 1712 5144
SexAI A'CCwGG_T
Cuts at: 2655 2655
Size: 6856
SfcI C'TryA_G
Cuts at: 826 1045 2276 2414 3628 4204 5339 6020 6211
Size: 219 1231 138 1214 576 1135 681 191
Cuts at: 6211 826
Size: 1471
Fragments arranged by size:
1471 1231 1214 1135 681 576 219 191
138
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6856
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6856
SmaI CCC'GGG
Cuts at: 1872 4183 1872
Size: 2311 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3641 4960 5861 6123 820
Size: 197 926 1698 1319 901 262 1553
Fragments arranged by size:
1698 1553 1319 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4153 4647 493
Size: 3660 494 2702
Fragments arranged by size:
3660 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6856
SphI G_CATG'C
Cuts at: 1132 4214 1132
Size: 3082 3774
SspI AAT'ATT
Cuts at: 5 52 2683 4780 5
Size: 47 2631 2097 2081
Fragments arranged by size:
2631 2097 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6856
StyI C'CwwG_G
Cuts at: 513 1065 2022 2310 2865 2888 3009 3904 4295
Size: 552 957 288 555 23 121 895 391
Cuts at: 4295 513
Size: 3074
Fragments arranged by size:
3074 957 895 555 552 391 288 121
23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2531 3022 4883 4900 5053 5238 5683
Size: 1255 951 491 1861 17 153 185 445
Cuts at: 5683 325
Size: 1498
Fragments arranged by size:
1861 1498 1255 951 491 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3002 3575 3768
Size: 274 80 33 51 1443 1025 573 193
Cuts at: 3768 5102 6814 96
Size: 1334 1712 138
Fragments arranged by size:
1712 1443 1334 1025 573 274 193 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2523 2555 3375 3407 3826 3858 4001 4033 6816
Size: 32 820 32 419 32 143 32 2783
Cuts at: 6816 6848 2523
Size: 32 2531
Fragments arranged by size:
2783 2531 820 419 143 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2657 3791 1316
Size: 1341 1134 4381
Fragments arranged by size:
4381 1341 1134
XbaI T'CTAG_A
Cuts at: 4192 4192
Size: 6856
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2144 2647 3751 2144
Size: 503 1104 5249
Fragments arranged by size:
5249 1104 503
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6856
XmaI C'CCGG_G
Cuts at: 1870 4181 1870
Size: 2311 4545
XmnI GAAnn'nnTTC
Cuts at: 4985 4985
Size: 6856
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4664 276
Size: 53 83 186 936 3130 2468
Fragments arranged by size:
3130 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SfiI SfoI SrfI SwaI