Restriction map for
pFN21ASDA1194
(Circular) (Six-base) MAPSORT of: pFN21ASDA1194.seq Check: 3672 from: 1 to: 6400
pFN21ASDA1194 6400 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4210 278
Size: 53 83 186 936 2674 2468
Fragments arranged by size:
2674 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2066 3743 1937
Size: 129 1677 4594
Fragments arranged by size:
4594 1677 129
Acc65I G'GTAC_C
Cuts at: 3721 3721
Size: 6400
AclI AA'CG_TT
Cuts at: 1563 4527 4900 1563
Size: 2964 373 3063
Fragments arranged by size:
3063 2964 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2208 2720 2794 2982 3352 4463 5847 6115
Size: 576 512 74 188 370 1111 1384 268
Cuts at: 6115 1632
Size: 1917
Fragments arranged by size:
1917 1384 1111 576 512 370 268 188
74
AflII C'TTAA_G
Cuts at: 820 1017 2149 820
Size: 197 1132 5071
Fragments arranged by size:
5071 1132 197
AflIII A'CryG_T
Cuts at: 1203 2857 5299 6276 1203
Size: 1654 2442 977 1327
Fragments arranged by size:
2442 1654 1327 977
AgeI A'CCGG_T
Cuts at: 5231 5231
Size: 6400
AhdI GACnn_n'nnGTC
Cuts at: 5129 5129
Size: 6400
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6400
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6400
AlwNI CAG_nnn'CTG
Cuts at: 2501 3278 3585 4143 5715 2501
Size: 777 307 558 1572 3186
Fragments arranged by size:
3186 1572 777 558 307
ApaI G_GGCC'C
Cuts at: 3545 3545
Size: 6400
ApaLI G'TGCA_C
Cuts at: 4457 5613 4457
Size: 1156 5244
ApoI r'AATT_y
Cuts at: 1493 2217 2277 2894 2924 2947 3224 3709 3935
Size: 724 60 617 30 23 277 485 226
Cuts at: 3935 4087 6196 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 724 617 485 277 226 152
60 30 23
AseI AT'TA_AT
Cuts at: 160 1032 4954 160
Size: 872 3922 1606
Fragments arranged by size:
3922 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6400
AvaI C'yCGr_G
Cuts at: 1870 1943 3725 1870
Size: 73 1782 4545
Fragments arranged by size:
4545 1782 73
BamHI G'GATC_C
Cuts at: 3730 4097 3730
Size: 367 6033
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3721 5176 618
Size: 325 217 627 1934 1455 1842
Fragments arranged by size:
1934 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2046 2328 3545 3642 3719 1391
Size: 655 282 1217 97 77 4072
Fragments arranged by size:
4072 1217 655 282 97 77
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2070 2726 928
Size: 971 171 656 4602
Fragments arranged by size:
4602 971 656 171
BbvCI CC'TCA_GC
Cuts at: 3516 3516
Size: 6400
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3025 3059 4591 4625 1903
Size: 34 1088 34 1532 34 3678
Fragments arranged by size:
3678 1532 1088 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4292 5502 4292
Size: 1210 5190
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6400
BfrBI ATG'CAT
Cuts at: 3109 3109
Size: 6400
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5011 136
Size: 107 122 71 631 605 3339 1525
Fragments arranged by size:
3339 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3256 6395 3256
Size: 3139 3261
BlpI GC'TnA_GC
Cuts at: 3817 3817
Size: 6400
Bme1580I G_kGCm'C
Cuts at: 1790 3545 4461 5617 1790
Size: 1755 916 1156 2573
Fragments arranged by size:
2573 1755 1156 916
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6400
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2122 2680 4131
Size: 416 53 83 399 24 705 558 1451
Cuts at: 4131 5089 442
Size: 958 1753
Fragments arranged by size:
1753 1451 958 705 558 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2939 1056
Size: 1883 4517
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3656 5060 1239
Size: 2417 1404 2579
Fragments arranged by size:
2579 2417 1404
Bpu10I CC'TnA_GC
Cuts at: 3516 3516
Size: 6400
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2728 4525 5390 5688 2728
Size: 1797 865 298 3440
Fragments arranged by size:
3440 1797 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5063 882
Size: 617 3564 2219
Fragments arranged by size:
3564 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3697 4191 493
Size: 711 2493 494 2702
Fragments arranged by size:
2702 2493 711 494
BsaBI GATnn'nnATC
Cuts at: 6394 6394
Size: 6400
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4207 4589 5224 275
Size: 53 83 186 936 2674 382 635 1451
Fragments arranged by size:
2674 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4833 5231 5505 5652 6063 4833
Size: 398 274 147 411 5170
Fragments arranged by size:
5170 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3329 3359 1178
Size: 30 165 30 1926 30 4219
Fragments arranged by size:
4219 1926 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6400
BseYI C'CCAG_C
Cuts at: 2368 5603 6099 2368
Size: 3235 496 2669
Fragments arranged by size:
3235 2669 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3273 1163
Size: 573 1537 4290
Fragments arranged by size:
4290 1537 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3850 4611 5639 1540
Size: 275 186 1849 761 1028 2301
Fragments arranged by size:
2301 1849 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2328 3302 3642 4461 4546 5617 2328
Size: 974 340 819 85 1071 3111
Fragments arranged by size:
3111 1071 974 819 340 85
BsmI GAATG_Cn'
Cuts at: 3911 4004 3911
Size: 93 6307
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2328 3302 3545 3642 3719 4461
Size: 399 256 282 974 243 97 77 742
Cuts at: 4461 4546 5617 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 974 742 399 282 256 243
97 85 77
BspEI T'CCGG_A
Cuts at: 6063 6063
Size: 6400
BspHI T'CATG_A
Cuts at: 2982 4289 6019 2982
Size: 1307 1730 3363
Fragments arranged by size:
3363 1730 1307
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3755 844
Size: 2911 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4287 1394
Size: 429 2464 3507
Fragments arranged by size:
3507 2464 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2229 2722 4895 5069 65
Size: 1183 981 493 2173 174 1396
Fragments arranged by size:
2173 1396 1183 981 493 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5044 5231 1700
Size: 110 3234 187 2869
Fragments arranged by size:
3234 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6400
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6400
BssSI C'ACGA_G
Cuts at: 4460 5472 4460
Size: 1012 5388
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6400
BstBI TT'CG_AA
Cuts at: 2007 5204 2007
Size: 3197 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6400
BstXI CCAn_nnnn'nTGG
Cuts at: 2541 3217 3683 2541
Size: 676 466 5258
Fragments arranged by size:
5258 676 466
BstYI r'GATC_y
Cuts at: 1972 3256 3730 4097 4482 4499 5951 6037 6049
Size: 1284 474 367 385 17 1452 86 12
Cuts at: 6049 6395 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1284 474 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2067 2067
Size: 6400
Bsu36I CC'TnA_GG
Cuts at: 3529 3529
Size: 6400
BtgI C'CryG_G
Cuts at: 513 1065 2940 513
Size: 552 1875 3973
Fragments arranged by size:
3973 1875 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5661
Fragments arranged by size:
5661 683 56
BtsI GCAGTG_nn'
Cuts at: 3916 4710 4730 3916
Size: 794 20 5586
Fragments arranged by size:
5586 794 20
ClaI AT'CG_AT
Cuts at: 1554 3035 1554
Size: 1481 4919
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3092 3127 529
Size: 35 2528 35 3802
Fragments arranged by size:
3802 2528 35 35
DraI TTT'AAA
Cuts at: 2334 3704 2334
Size: 1370 5030
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5407 809
Size: 507 4091 1802
Fragments arranged by size:
4091 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3847 4736 6365
Size: 54 1425 50 275 18 2017 889 1629
Cuts at: 6365 8
Size: 43
Fragments arranged by size:
2017 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3847 1537
Size: 275 2035 4090
Fragments arranged by size:
4090 2035 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4330 1353
Size: 2977 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4987 5361 5507 387
Size: 1064 138 3398 374 146 1280
Fragments arranged by size:
3398 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2326 3640 2326
Size: 1314 5086
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2208 2720 2794 2982 3352 3656 4463
Size: 393 576 512 74 188 370 304 807
Cuts at: 4463 5060 5847 6115 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 807 787 597 576 512 393 370
304 268 188 74
EcoNI CCTnn'n_nnAGG
Cuts at: 2420 2441 2420
Size: 21 6379
EcoO109I rG'GnC_Cy
Cuts at: 3115 3542 3115
Size: 427 5973
EcoRI G'AATT_C
Cuts at: 2217 3709 4087 2217
Size: 1492 378 4530
Fragments arranged by size:
4530 1492 378
EcoRV GAT'ATC
Cuts at: 2725 2725
Size: 6400
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2175 2175
Size: 6400
FspI TGC'GCA
Cuts at: 4906 4906
Size: 6400
HaeII r_GCGC'y
Cuts at: 1678 2666 5547 1678
Size: 988 2881 2531
Fragments arranged by size:
2881 2531 988
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2252 2284 2477 2509 3176
Size: 32 135 32 680 32 193 32 667
Cuts at: 3176 3208 3602 3634 5041 5073 5115 5147 1373
Size: 32 394 32 1407 32 42 32 2626
Fragments arranged by size:
2626 1407 680 667 394 193 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3667 3744 3990 669
Size: 988 281 1729 77 246 3079
Fragments arranged by size:
3079 1729 988 281 246 77
HindIII A'AGCT_T
Cuts at: 748 2146 2237 748
Size: 1398 91 4911
Fragments arranged by size:
4911 1398 91
HpaI GTT'AAC
Cuts at: 3990 3990
Size: 6400
KpnI G_GTAC'C
Cuts at: 3725 3725
Size: 6400
MfeI C'AATT_G
Cuts at: 3999 3999
Size: 6400
MluI A'CGCG_T
Cuts at: 6276 6276
Size: 6400
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5514 5698 1305
Size: 4209 184 2007
Fragments arranged by size:
4209 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4578
Fragments arranged by size:
4578 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2161 2196 2862 4358 4717 4876 518
Size: 648 995 35 666 1496 359 159 2042
Fragments arranged by size:
2042 1496 995 666 648 359 159 35
MspA1I CmG'CkG
Cuts at: 1606 3372 3520 3816 4493 5641 5886 1606
Size: 1766 148 296 677 1148 245 2120
Fragments arranged by size:
2120 1766 1148 677 296 245 148
NcoI C'CATG_G
Cuts at: 513 1065 2940 513
Size: 552 1875 3973
Fragments arranged by size:
3973 1875 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6400
NheI G'CTAG_C
Cuts at: 1052 2935 1052
Size: 1883 4517
NotI GC'GGCC_GC
Cuts at: 3847 3847
Size: 6400
NsiI A_TGCA'T
Cuts at: 3111 3111
Size: 6400
NspI r_CATG'y
Cuts at: 1132 1229 2861 3109 3758 5303 1132
Size: 97 1632 248 649 1545 2229
Fragments arranged by size:
2229 1632 1545 649 248 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6400
PciI A'CATG_T
Cuts at: 2857 5299 2857
Size: 2442 3958
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3065 3278 3562 1108
Size: 1957 213 284 3946
Fragments arranged by size:
3946 1957 284 213
PmeI GTTT'AAAC
Cuts at: 3704 3704
Size: 6400
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4469 4501 5982 6014 4469
Size: 32 1481 32 4855
Fragments arranged by size:
4855 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3115 3115
Size: 6400
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6400
PsiI TTA'TAA
Cuts at: 3970 3970
Size: 6400
PspOMI G'GGCC_C
Cuts at: 3541 3541
Size: 6400
PstI C_TGCA'G
Cuts at: 830 3752 830
Size: 2922 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6400
PvuII CAG'CTG
Cuts at: 3372 3520 3372
Size: 148 6252
SacI G_AGCT'C
Cuts at: 2328 3642 2328
Size: 1314 5086
SalI G'TCGA_C
Cuts at: 1936 3742 1936
Size: 1806 4594
SanDI GG'GwC_CC
Cuts at: 3115 3115
Size: 6400
SbfI CC_TGCA'GG
Cuts at: 3752 3752
Size: 6400
ScaI AGT'ACT
Cuts at: 3012 4648 6360 3012
Size: 1636 1712 3052
Fragments arranged by size:
3052 1712 1636
SfcI C'TryA_G
Cuts at: 826 1045 2677 3748 4883 5564 5755 826
Size: 219 1632 1071 1135 681 191 1471
Fragments arranged by size:
1632 1471 1135 1071 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6400
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6400
SmaI CCC'GGG
Cuts at: 1872 3727 1872
Size: 1855 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2149 2707 4504 5405 5667 820
Size: 197 926 206 558 1797 901 262 1553
Fragments arranged by size:
1797 1553 926 901 558 262 206 197
SnaBI TAC'GTA
Cuts at: 493 3697 4191 493
Size: 3204 494 2702
Fragments arranged by size:
3204 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6400
SphI G_CATG'C
Cuts at: 1132 3758 1132
Size: 2626 3774
SspI AAT'ATT
Cuts at: 5 52 2877 3293 4324 5
Size: 47 2825 416 1031 2081
Fragments arranged by size:
2825 2081 1031 416 47
StuI AGG'CCT
Cuts at: 1517 2400 1517
Size: 883 5517
StyI C'CwwG_G
Cuts at: 513 1065 2534 2940 3839 513
Size: 552 1469 406 899 3074
Fragments arranged by size:
3074 1469 899 552 406
SwaI ATTT'AAAT
Cuts at: 2334 2334
Size: 6400
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4427 4444 4597 4782 5227 325
Size: 1255 2847 17 153 185 445 1498
Fragments arranged by size:
2847 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3010 4646 6358
Size: 274 80 33 51 1443 1033 1636 1712
Cuts at: 6358 96
Size: 138
Fragments arranged by size:
1712 1636 1443 1033 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6360 6392 6360
Size: 32 6368
Tth111I GACn'n_nGTC
Cuts at: 1316 2836 1316
Size: 1520 4880
XbaI T'CTAG_A
Cuts at: 2822 3736 2822
Size: 914 5486
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6400
XmaI C'CCGG_G
Cuts at: 1870 3725 1870
Size: 1855 4545
XmnI GAAnn'nnTTC
Cuts at: 2221 4529 2221
Size: 2308 4092
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4208 276
Size: 53 83 186 936 2674 2468
Fragments arranged by size:
2674 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI DraIII FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PspXI PsrI RsrII
SacII SapI SexAI SfiI SfoI SrfI XcmI