Restriction map for
pFN21ASDA1184
(Circular) (Six-base) MAPSORT of: pFN21ASDA1184.seq Check: 8195 from: 1 to: 5884
pFN21ASDA1184 5884 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2915 2915
Size: 5884
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3694 278
Size: 53 83 186 936 2158 2468
Fragments arranged by size:
2468 2158 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2727 3227 1937
Size: 790 500 4594
Fragments arranged by size:
4594 790 500
Acc65I G'GTAC_C
Cuts at: 2289 3205 2289
Size: 916 4968
AclI AA'CG_TT
Cuts at: 1563 4011 4384 1563
Size: 2448 373 3063
Fragments arranged by size:
3063 2448 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2072 2451 2663 3176 3947 5331 5599 1632
Size: 440 379 212 513 771 1384 268 1917
Fragments arranged by size:
1917 1384 771 513 440 379 268 212
AfeI AGC'GCT
Cuts at: 3032 3032
Size: 5884
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5687
AflIII A'CryG_T
Cuts at: 1203 4783 5760 1203
Size: 3580 977 1327
Fragments arranged by size:
3580 1327 977
AgeI A'CCGG_T
Cuts at: 4715 4715
Size: 5884
AhdI GACnn_n'nnGTC
Cuts at: 4613 4613
Size: 5884
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5884
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5884
AlwNI CAG_nnn'CTG
Cuts at: 2311 2989 3136 3627 5199 2311
Size: 678 147 491 1572 2996
Fragments arranged by size:
2996 1572 678 491 147
ApaI G_GGCC'C
Cuts at: 2143 2692 3101 3130 2143
Size: 549 409 29 4897
Fragments arranged by size:
4897 549 409 29
ApaLI G'TGCA_C
Cuts at: 3941 5097 3941
Size: 1156 4728
ApoI r'AATT_y
Cuts at: 1493 3193 3419 3571 5680 1493
Size: 1700 226 152 2109 1697
Fragments arranged by size:
2109 1700 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4438 160
Size: 872 3406 1606
Fragments arranged by size:
3406 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5884
AvaI C'yCGr_G
Cuts at: 1870 1943 2136 2285 2475 2939 2952 3123 3209
Size: 73 193 149 190 464 13 171 86
Cuts at: 3209 1870
Size: 4545
Fragments arranged by size:
4545 464 193 190 171 149 86 73
13
BamHI G'GATC_C
Cuts at: 3214 3581 3214
Size: 367 5517
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2289 2583 3205 4660 618
Size: 325 217 627 502 294 622 1455 1842
Fragments arranged by size:
1842 1455 627 622 502 325 294 217
BanII G_rGCy'C
Cuts at: 1391 2143 2323 2612 2692 2748 2843 2940 3101
Size: 752 180 289 80 56 95 97 161
Cuts at: 3101 3130 3147 3203 1391
Size: 29 17 56 4072
Fragments arranged by size:
4072 752 289 180 161 97 95 80
56 56 29 17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4913
BbvCI CC'TCA_GC
Cuts at: 2573 2573
Size: 5884
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2266 2300 4075 4109 1903
Size: 34 329 34 1775 34 3678
Fragments arranged by size:
3678 1775 329 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3776 4986 3776
Size: 1210 4674
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5884
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2447 4495 136
Size: 107 122 71 631 605 775 2048 1525
Fragments arranged by size:
2048 1525 775 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5879 5879
Size: 5884
BlpI GC'TnA_GC
Cuts at: 2837 3301 2837
Size: 464 5420
Bme1580I G_kGCm'C
Cuts at: 1790 2143 2692 3005 3011 3101 3130 3945 5101
Size: 353 549 313 6 90 29 815 1156
Cuts at: 5101 1790
Size: 2573
Fragments arranged by size:
2573 1156 815 549 353 313 90 29
6
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5884
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2554 3114 3615
Size: 416 53 83 399 24 1137 560 501
Cuts at: 3615 4573 442
Size: 958 1753
Fragments arranged by size:
1753 1137 958 560 501 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5884
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3049 3049
Size: 5884
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3011 4544 1239
Size: 1772 1533 2579
Fragments arranged by size:
2579 1772 1533
Bpu10I CC'TnA_GC
Cuts at: 2573 2573
Size: 5884
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4009 4874 5172 4009
Size: 865 298 4721
Fragments arranged by size:
4721 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2822 4547 882
Size: 617 1323 1725 2219
Fragments arranged by size:
2219 1725 1323 617
BsaAI yAC'GTr
Cuts at: 493 1204 3006 3181 3675 493
Size: 711 1802 175 494 2702
Fragments arranged by size:
2702 1802 711 494 175
BsaBI GATnn'nnATC
Cuts at: 5878 5878
Size: 5884
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3691 4073 4708 275
Size: 53 83 186 936 2158 382 635 1451
Fragments arranged by size:
2158 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4317 4715 4989 5136 5547 4317
Size: 398 274 147 411 4654
Fragments arranged by size:
4654 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2367 2397 1178
Size: 30 165 30 964 30 4665
Fragments arranged by size:
4665 964 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2921 3041 1186
Size: 1735 120 4029
Fragments arranged by size:
4029 1735 120
BseYI C'CCAG_C
Cuts at: 2355 2442 2857 3129 5087 5583 2355
Size: 87 415 272 1958 496 2656
Fragments arranged by size:
2656 1958 496 415 272 87
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2504 2996 1163
Size: 573 768 492 4051
Fragments arranged by size:
4051 768 573 492
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3334 4095 5123 1540
Size: 275 186 1333 761 1028 2301
Fragments arranged by size:
2301 1333 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2843 3147 3945 4030 5101 2843
Size: 304 798 85 1071 3626
Fragments arranged by size:
3626 1071 798 304 85
BsmI GAATG_Cn'
Cuts at: 3395 3488 3395
Size: 93 5791
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2143 2323 2612 2692 2748 2843 2940
Size: 399 353 180 289 80 56 95 97
Cuts at: 2940 3005 3011 3101 3130 3147 3203 3945 4030
Size: 65 6 90 29 17 56 742 85
Cuts at: 4030 5101 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 353 289 180 97
95 90 85 80 65 56 56 29
17 6
BspEI T'CCGG_A
Cuts at: 5547 5547
Size: 5884
BspHI T'CATG_A
Cuts at: 2893 3773 5503 2893
Size: 880 1730 3274
Fragments arranged by size:
3274 1730 880
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2790 2915 3239 844
Size: 1946 125 324 3489
Fragments arranged by size:
3489 1946 324 125
BsrBI CCG'CTC
Cuts at: 1394 1823 3771 1394
Size: 429 1948 3507
Fragments arranged by size:
3507 1948 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2827 4379 4553 65
Size: 1183 1579 1552 174 1396
Fragments arranged by size:
1579 1552 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2577 3150 4528 4715 1700
Size: 110 767 573 1378 187 2869
Fragments arranged by size:
2869 1378 767 573 187 110
BsrGI T'GTAC_A
Cuts at: 96 2268 96
Size: 2172 3712
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5884
BssSI C'ACGA_G
Cuts at: 3944 4956 3944
Size: 1012 4872
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2974 794
Size: 2180 3704
BstBI TT'CG_AA
Cuts at: 2007 4688 2007
Size: 2681 3203
BstEII G'GTnAC_C
Cuts at: 1184 2509 1184
Size: 1325 4559
BstYI r'GATC_y
Cuts at: 1972 2682 3214 3581 3966 3983 5435 5521 5533
Size: 710 532 367 385 17 1452 86 12
Cuts at: 5533 5879 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 710 532 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2613 2613
Size: 5884
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4375
Fragments arranged by size:
4375 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5145
Fragments arranged by size:
5145 683 56
BtsI GCAGTG_nn'
Cuts at: 2975 3058 3400 4194 4214 2975
Size: 83 342 794 20 4645
Fragments arranged by size:
4645 794 342 83 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5884
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5849
DraI TTT'AAA
Cuts at: 3188 3188
Size: 5884
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4891 809
Size: 507 3575 1802
Fragments arranged by size:
3575 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2704 3331 4220
Size: 54 1425 50 275 18 874 627 889
Cuts at: 4220 5849 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 874 627 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3331 1537
Size: 275 1519 4090
Fragments arranged by size:
4090 1519 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2300 2372 2774 2792 3135 3814 1353
Size: 947 72 402 18 343 679 3423
Fragments arranged by size:
3423 947 679 402 343 72 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2821 3137 4471 4845 4991 387
Size: 1064 138 1232 316 1334 374 146 1280
Fragments arranged by size:
1334 1280 1232 1064 374 316 146 138
EcoICRI GAG'CTC
Cuts at: 2841 3145 2841
Size: 304 5580
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2451 2663 3011 3176 3947 4544
Size: 393 440 379 212 348 165 771 597
Cuts at: 4544 5331 5599 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 771 597 440 393 379 348
268 212 165
EcoNI CCTnn'n_nnAGG
Cuts at: 3023 3023
Size: 5884
EcoO109I rG'GnC_Cy
Cuts at: 2133 2140 2689 2756 2779 3098 2133
Size: 7 549 67 23 319 4919
Fragments arranged by size:
4919 549 319 67 23 7
EcoRI G'AATT_C
Cuts at: 3193 3571 3193
Size: 378 5506
FspI TGC'GCA
Cuts at: 2315 4390 2315
Size: 2075 3809
HaeII r_GCGC'y
Cuts at: 1678 2051 3034 3071 5031 1678
Size: 373 983 37 1960 2531
Fragments arranged by size:
2531 1960 983 373 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3104 3136 4525 4557 4599
Size: 32 135 32 1532 32 1389 32 42
Cuts at: 4599 4631 1373
Size: 32 2626
Fragments arranged by size:
2626 1532 1389 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3228 3474 669
Size: 988 281 1290 246 3079
Fragments arranged by size:
3079 1290 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5884
HpaI GTT'AAC
Cuts at: 3474 3474
Size: 5884
KpnI G_GTAC'C
Cuts at: 2293 3209 2293
Size: 916 4968
MfeI C'AATT_G
Cuts at: 3483 3483
Size: 5884
MluI A'CGCG_T
Cuts at: 5760 5760
Size: 5884
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4998 5182 1305
Size: 3693 184 2007
Fragments arranged by size:
3693 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2706 10
Size: 54 1425 343 874 3188
Fragments arranged by size:
3188 1425 874 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2655 2898 3842 4201 4360 518
Size: 648 1489 243 944 359 159 2042
Fragments arranged by size:
2042 1489 944 648 359 243 159
MspA1I CmG'CkG
Cuts at: 1606 2311 2446 2980 2986 3300 3977 5125 5370
Size: 705 135 534 6 314 677 1148 245
Cuts at: 5370 1606
Size: 2120
Fragments arranged by size:
2120 1148 705 677 534 314 245 135
6
NaeI GCC'GGC
Cuts at: 2579 3152 2579
Size: 573 5311
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4375
Fragments arranged by size:
4375 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5884
NgoMIV G'CCGG_C
Cuts at: 2577 3150 2577
Size: 573 5311
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5884
NotI GC'GGCC_GC
Cuts at: 3331 3331
Size: 5884
NspI r_CATG'y
Cuts at: 1132 1229 3242 4787 1132
Size: 97 2013 1545 2229
Fragments arranged by size:
2229 2013 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3019 1348
Size: 1671 4213
PciI A'CATG_T
Cuts at: 4783 4783
Size: 5884
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3064 3136 1108
Size: 1956 72 3856
Fragments arranged by size:
3856 1956 72
PfoI T'CCnGG_A
Cuts at: 3135 3135
Size: 5884
PmeI GTTT'AAAC
Cuts at: 3188 3188
Size: 5884
PmlI CAC'GTG
Cuts at: 3006 3006
Size: 5884
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3953 3985 5466 5498 3953
Size: 32 1481 32 4339
Fragments arranged by size:
4339 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2133 2756 2779 2133
Size: 623 23 5238
Fragments arranged by size:
5238 623 23
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5884
PsiI TTA'TAA
Cuts at: 3454 3454
Size: 5884
PspOMI G'GGCC_C
Cuts at: 2139 2688 3097 3126 2139
Size: 549 409 29 4897
Fragments arranged by size:
4897 549 409 29
PstI C_TGCA'G
Cuts at: 830 3236 830
Size: 2406 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5884
PvuII CAG'CTG
Cuts at: 2311 2446 2311
Size: 135 5749
SacI G_AGCT'C
Cuts at: 2843 3147 2843
Size: 304 5580
SalI G'TCGA_C
Cuts at: 1936 3226 1936
Size: 1290 4594
SanDI GG'GwC_CC
Cuts at: 2756 2756
Size: 5884
SapI GCTCTTCn'nnn_
Cuts at: 2792 3135 2792
Size: 343 5541
SbfI CC_TGCA'GG
Cuts at: 3236 3236
Size: 5884
ScaI AGT'ACT
Cuts at: 4132 5844 4132
Size: 1712 4172
SfcI C'TryA_G
Cuts at: 826 1045 2413 3071 3232 4367 5048 5239 826
Size: 219 1368 658 161 1135 681 191 1471
Fragments arranged by size:
1471 1368 1135 681 658 219 191 161
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5884
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5884
SmaI CCC'GGG
Cuts at: 1872 2138 2954 3125 3211 1872
Size: 266 816 171 86 4545
Fragments arranged by size:
4545 816 266 171 86
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3988 4889 5151 820
Size: 197 926 2045 901 262 1553
Fragments arranged by size:
2045 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3181 3675 493
Size: 2688 494 2702
Fragments arranged by size:
2702 2688 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5884
SphI G_CATG'C
Cuts at: 1132 3242 1132
Size: 2110 3774
SspI AAT'ATT
Cuts at: 5 52 3808 5
Size: 47 3756 2081
Fragments arranged by size:
3756 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5884
StyI C'CwwG_G
Cuts at: 513 1065 2022 2514 3323 513
Size: 552 957 492 809 3074
Fragments arranged by size:
3074 957 809 552 492
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2450 2651 2962 3911 3928 4081 4266
Size: 1255 870 201 311 949 17 153 185
Cuts at: 4266 4711 325
Size: 445 1498
Fragments arranged by size:
1498 1255 949 870 445 311 201 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2268 2531 4130
Size: 274 80 33 51 1443 291 263 1599
Cuts at: 4130 5842 96
Size: 1712 138
Fragments arranged by size:
1712 1599 1443 291 274 263 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2367 2399 5844 5876 2367
Size: 32 3445 32 2375
Fragments arranged by size:
3445 2375 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5884
XbaI T'CTAG_A
Cuts at: 3220 3220
Size: 5884
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 2242 2029
Size: 213 5671
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5884
XmaI C'CCGG_G
Cuts at: 1870 2136 2952 3123 3209 1870
Size: 266 816 171 86 4545
Fragments arranged by size:
4545 816 266 171 86
XmnI GAAnn'nnTTC
Cuts at: 2208 4013 2208
Size: 1805 4079
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3692 276
Size: 53 83 186 936 2158 2468
Fragments arranged by size:
2468 2158 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstXI BstZ17I DraIII EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PspXI PsrI RsrII SacII
SexAI SfiI SfoI SrfI SwaI