Restriction map for
pFN21ASDA1169
(Circular) (Six-base) MAPSORT of: pFN21ASDA1169.seq Check: 868 from: 1 to: 7177
pFN21ASDA1169 7177 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4987 278
Size: 53 83 186 936 3451 2468
Fragments arranged by size:
3451 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4520 1937
Size: 2583 4594
Acc65I G'GTAC_C
Cuts at: 4498 4498
Size: 7177
AclI AA'CG_TT
Cuts at: 1563 5304 5677 1563
Size: 3741 373 3063
Fragments arranged by size:
3741 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2781 2794 3518 4352 5240 6624 6892 1632
Size: 1149 13 724 834 888 1384 268 1917
Fragments arranged by size:
1917 1384 1149 888 834 724 268 13
AfeI AGC'GCT
Cuts at: 3158 3677 4454 3158
Size: 519 777 5881
Fragments arranged by size:
5881 777 519
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6980
AflIII A'CryG_T
Cuts at: 1203 6076 7053 1203
Size: 4873 977 1327
Fragments arranged by size:
4873 1327 977
AgeI A'CCGG_T
Cuts at: 6008 6008
Size: 7177
AhdI GACnn_n'nnGTC
Cuts at: 5906 5906
Size: 7177
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7177
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2653 1184
Size: 1469 5708
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2891 2923 3419 3451 3686 3718 2891
Size: 32 496 32 235 32 6350
Fragments arranged by size:
6350 496 235 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2707 3652 4920 6492 2707
Size: 945 1268 1572 3392
Fragments arranged by size:
3392 1572 1268 945
ApaI G_GGCC'C
Cuts at: 3518 3518
Size: 7177
ApaLI G'TGCA_C
Cuts at: 5234 6390 5234
Size: 1156 6021
ApoI r'AATT_y
Cuts at: 1493 4486 4712 4864 6973 1493
Size: 2993 226 152 2109 1697
Fragments arranged by size:
2993 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3324 5731 160
Size: 872 2292 2407 1606
Fragments arranged by size:
2407 2292 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7177
AvaI C'yCGr_G
Cuts at: 1870 1943 2625 4082 4502 1870
Size: 73 682 1457 420 4545
Fragments arranged by size:
4545 1457 682 420 73
BamHI G'GATC_C
Cuts at: 3393 4507 4874 3393
Size: 1114 367 5696
Fragments arranged by size:
5696 1114 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4498 5953 618
Size: 325 217 627 2711 1455 1842
Fragments arranged by size:
2711 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3518 3615 3951 4200 4496 1391
Size: 2127 97 336 249 296 4072
Fragments arranged by size:
4072 2127 336 296 249 97
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2908 3525 3793 4087 928
Size: 971 1009 617 268 294 4018
Fragments arranged by size:
4018 1009 971 617 294 268
BbvCI CC'TCA_GC
Cuts at: 3653 3653
Size: 7177
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3221 3255 5368 5402 1903
Size: 34 1284 34 2113 34 3678
Fragments arranged by size:
3678 2113 1284 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5069 6279 5069
Size: 1210 5967
BclI T'GATC_A
Cuts at: 1548 2751 1548
Size: 1203 5974
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2090 5788 136
Size: 107 122 71 631 605 418 3698 1525
Fragments arranged by size:
3698 1525 631 605 418 122 107 71
BglII A'GATC_T
Cuts at: 2694 7172 2694
Size: 4478 2699
BlpI GC'TnA_GC
Cuts at: 3203 4594 3203
Size: 1391 5786
Bme1580I G_kGCm'C
Cuts at: 1790 2086 3450 3518 5238 6394 1790
Size: 296 1364 68 1720 1156 2573
Fragments arranged by size:
2573 1720 1364 1156 296 68
BmgBI CAC'GTC
Cuts at: 1318 2047 1318
Size: 729 6448
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2166 2230 4908
Size: 416 53 83 399 24 749 64 2678
Cuts at: 4908 5866 442
Size: 958 1753
Fragments arranged by size:
2678 1753 958 749 416 399 83 64
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7177
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4270 4270
Size: 7177
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2188 2240 2471 2840 3472 3629 4030 4214
Size: 949 52 231 369 632 157 401 184
Cuts at: 4214 4265 5837 1239
Size: 51 1572 2579
Fragments arranged by size:
2579 1572 949 632 401 369 231 184
157 52 51
Bpu10I CC'TnA_GC
Cuts at: 3653 4325 3653
Size: 672 6505
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4175 5302 6167 6465 4175
Size: 1127 865 298 4887
Fragments arranged by size:
4887 1127 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4241 5840 882
Size: 617 2742 1599 2219
Fragments arranged by size:
2742 2219 1599 617
BsaAI yAC'GTr
Cuts at: 493 1204 3445 4474 4968 493
Size: 711 2241 1029 494 2702
Fragments arranged by size:
2702 2241 1029 711 494
BsaBI GATnn'nnATC
Cuts at: 4061 7171 4061
Size: 3110 4067
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2673 3987 4984 5366
Size: 53 83 186 936 1140 1314 997 382
Cuts at: 5366 6001 275
Size: 635 1451
Fragments arranged by size:
1451 1314 1140 997 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3536 5610 6008 6282 6429 6840 3536
Size: 2074 398 274 147 411 3873
Fragments arranged by size:
3873 2074 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2768 2798 3419 3449 3515
Size: 30 165 30 1365 30 621 30 66
Cuts at: 3515 3545 1178
Size: 30 4810
Fragments arranged by size:
4810 1365 621 165 66 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3094 3296 4213 4361 4382 1186
Size: 1908 202 917 148 21 3981
Fragments arranged by size:
3981 1908 917 202 148 21
BseYI C'CCAG_C
Cuts at: 2506 4428 6380 6876 2506
Size: 1922 1952 496 2807
Fragments arranged by size:
2807 1952 1922 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2588 3014 4434 1163
Size: 573 852 426 1420 3906
Fragments arranged by size:
3906 1420 852 573 426
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4627 5388 6416 1540
Size: 275 186 2626 761 1028 2301
Fragments arranged by size:
2626 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2632 3276 3951 4200 5238 5323 6394 2632
Size: 644 675 249 1038 85 1071 3415
Fragments arranged by size:
3415 1071 1038 675 644 249 85
BsmI GAATG_Cn'
Cuts at: 4688 4781 4688
Size: 93 7084
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2086 2632 3276 3450 3518 3615 3951
Size: 399 296 546 644 174 68 97 336
Cuts at: 3951 4200 4496 5238 5323 6394 1391
Size: 249 296 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 644 546 399 336 296
296 249 174 97 85 68
BspEI T'CCGG_A
Cuts at: 3536 6840 3536
Size: 3304 3873
BspHI T'CATG_A
Cuts at: 2748 2955 4026 5066 6796 2748
Size: 207 1071 1040 1730 3129
Fragments arranged by size:
3129 1730 1071 1040 207
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2694 2970 4532 844
Size: 1850 276 1562 3489
Fragments arranged by size:
3489 1850 1562 276
BsrBI CCG'CTC
Cuts at: 1394 1823 2053 5064 1394
Size: 429 230 3011 3507
Fragments arranged by size:
3507 3011 429 230
BsrDI GCAATG_nn'
Cuts at: 65 1248 5672 5846 65
Size: 1183 4424 174 1396
Fragments arranged by size:
4424 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3089 3506 5821 6008 1700
Size: 110 1279 417 2315 187 2869
Fragments arranged by size:
2869 2315 1279 417 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7177
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7177
BssSI C'ACGA_G
Cuts at: 3398 3820 5237 6249 3398
Size: 422 1417 1012 4326
Fragments arranged by size:
4326 1417 1012 422
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7177
BstBI TT'CG_AA
Cuts at: 2007 5981 2007
Size: 3974 3203
BstEII G'GTnAC_C
Cuts at: 1184 2620 1184
Size: 1436 5741
BstXI CCAn_nnnn'nTGG
Cuts at: 2107 2311 4243 2107
Size: 204 1932 5041
Fragments arranged by size:
5041 1932 204
BstYI r'GATC_y
Cuts at: 1972 2694 3393 4350 4507 4874 5259 5276 6728
Size: 722 699 957 157 367 385 17 1452
Cuts at: 6728 6814 6826 7172 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 957 722 699 385 367 346
157 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2364 2541 3861 3876 4047 4284
Size: 552 957 342 177 1320 15 171 237
Cuts at: 4284 513
Size: 3406
Fragments arranged by size:
3406 1320 957 552 342 237 177 171
15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6438
Fragments arranged by size:
6438 683 56
BtsI GCAGTG_nn'
Cuts at: 2086 2630 4417 4693 5487 5507 2086
Size: 544 1787 276 794 20 3756
Fragments arranged by size:
3756 1787 794 544 276 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7177
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7142
DraI TTT'AAA
Cuts at: 4481 4481
Size: 7177
DraIII CAC_nnn'GTG
Cuts at: 3862 3862
Size: 7177
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6184 809
Size: 507 4868 1802
Fragments arranged by size:
4868 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2361 3723 4624
Size: 54 1425 50 275 18 531 1362 901
Cuts at: 4624 5513 7142 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1362 901 889 531 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4624 1537
Size: 275 2812 4090
Fragments arranged by size:
4090 2812 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2112 2348 4170 5107 1353
Size: 759 236 1822 937 3423
Fragments arranged by size:
3423 1822 937 759 236
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5764 6138 6284 387
Size: 1064 138 4175 374 146 1280
Fragments arranged by size:
4175 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3949 4198 3949
Size: 249 6928
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2188 2240 2471 2781 2794 2840 3472
Size: 393 556 52 231 310 13 46 632
Cuts at: 3472 3518 3629 4030 4214 4265 4352 5240 5837
Size: 46 111 401 184 51 87 888 597
Cuts at: 5837 6624 6892 1239
Size: 787 268 1524
Fragments arranged by size:
1524 888 787 632 597 556 401 393
310 268 231 184 111 87 52 51
46 46 13
EcoNI CCTnn'n_nnAGG
Cuts at: 2396 4157 2396
Size: 1761 5416
EcoO109I rG'GnC_Cy
Cuts at: 2167 2401 3175 3515 4218 2167
Size: 234 774 340 703 5126
Fragments arranged by size:
5126 774 703 340 234
EcoRI G'AATT_C
Cuts at: 4486 4864 4486
Size: 378 6799
EcoRV GAT'ATC
Cuts at: 3226 3226
Size: 7177
FspI TGC'GCA
Cuts at: 5683 5683
Size: 7177
HaeII r_GCGC'y
Cuts at: 1678 2312 3160 3305 3596 3679 4456 6324 1678
Size: 634 848 145 291 83 777 1868 2531
Fragments arranged by size:
2531 1868 848 777 634 291 145 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3515 3547 5818 5850 5892
Size: 32 135 32 1943 32 2271 32 42
Cuts at: 5892 5924 1373
Size: 32 2626
Fragments arranged by size:
2626 2271 1943 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3382 3961 4521 4767 669
Size: 988 281 1444 579 560 246 3079
Fragments arranged by size:
3079 1444 988 579 560 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7177
HpaI GTT'AAC
Cuts at: 4767 4767
Size: 7177
KpnI G_GTAC'C
Cuts at: 4502 4502
Size: 7177
MfeI C'AATT_G
Cuts at: 4776 4776
Size: 7177
MluI A'CGCG_T
Cuts at: 7053 7053
Size: 7177
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2124 6291 6475 1305
Size: 819 4167 184 2007
Fragments arranged by size:
4167 2007 819 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2363 3725 10
Size: 54 1425 343 531 1362 3462
Fragments arranged by size:
3462 1425 1362 531 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2588 3977 4052 5135 5494 5653
Size: 648 861 561 1389 75 1083 359 159
Cuts at: 5653 518
Size: 2042
Fragments arranged by size:
2042 1389 1083 861 648 561 359 159
75
MspA1I CmG'CkG
Cuts at: 1606 3649 3846 4593 5270 6418 6663 1606
Size: 2043 197 747 677 1148 245 2120
Fragments arranged by size:
2120 2043 1148 747 677 245 197
NaeI GCC'GGC
Cuts at: 3091 3508 3091
Size: 417 6760
NcoI C'CATG_G
Cuts at: 513 1065 2022 2541 4047 4284 513
Size: 552 957 519 1506 237 3406
Fragments arranged by size:
3406 1506 957 552 519 237
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7177
NgoMIV G'CCGG_C
Cuts at: 3089 3506 3089
Size: 417 6760
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7177
NotI GC'GGCC_GC
Cuts at: 4624 4624
Size: 7177
NspI r_CATG'y
Cuts at: 1132 1229 3056 4376 4535 6080 1132
Size: 97 1827 1320 159 1545 2229
Fragments arranged by size:
2229 1827 1545 1320 159 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7177
PciI A'CATG_T
Cuts at: 6076 6076
Size: 7177
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3862 1108
Size: 2754 4423
PfoI T'CCnGG_A
Cuts at: 2256 2894 4191 4353 2256
Size: 638 1297 162 5080
Fragments arranged by size:
5080 1297 638 162
PmeI GTTT'AAAC
Cuts at: 4481 4481
Size: 7177
PmlI CAC'GTG
Cuts at: 3445 3445
Size: 7177
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3419 3451 4112 4144 5246 5278 6759 6791 3419
Size: 32 661 32 1102 32 1481 32 3805
Fragments arranged by size:
3805 1481 1102 661 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2543 1613
Size: 930 6247
PsiI TTA'TAA
Cuts at: 4747 4747
Size: 7177
PspOMI G'GGCC_C
Cuts at: 3514 3514
Size: 7177
PspXI vC'TCGA_Gb
Cuts at: 4082 4082
Size: 7177
PstI C_TGCA'G
Cuts at: 830 4529 830
Size: 3699 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7177
PvuII CAG'CTG
Cuts at: 3649 3649
Size: 7177
SacI G_AGCT'C
Cuts at: 3951 4200 3951
Size: 249 6928
SalI G'TCGA_C
Cuts at: 1936 4519 1936
Size: 2583 4594
SapI GCTCTTCn'nnn_
Cuts at: 2112 4170 2112
Size: 2058 5119
SbfI CC_TGCA'GG
Cuts at: 4529 4529
Size: 7177
ScaI AGT'ACT
Cuts at: 3527 5425 7137 3527
Size: 1898 1712 3567
Fragments arranged by size:
3567 1898 1712
SfcI C'TryA_G
Cuts at: 826 1045 3100 4108 4525 5660 6341 6532 826
Size: 219 2055 1008 417 1135 681 191 1471
Fragments arranged by size:
2055 1471 1135 1008 681 417 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7177
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7177
SmaI CCC'GGG
Cuts at: 1872 4504 1872
Size: 2632 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4082 4154 5281 6182 6444 820
Size: 197 926 2139 72 1127 901 262 1553
Fragments arranged by size:
2139 1553 1127 926 901 262 197 72
SnaBI TAC'GTA
Cuts at: 493 4474 4968 493
Size: 3981 494 2702
Fragments arranged by size:
3981 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7177
SphI G_CATG'C
Cuts at: 1132 3056 4376 4535 1132
Size: 1924 1320 159 3774
Fragments arranged by size:
3774 1924 1320 159
SspI AAT'ATT
Cuts at: 5 52 5101 5
Size: 47 5049 2081
Fragments arranged by size:
5049 2081 47
StuI AGG'CCT
Cuts at: 1517 3085 3117 3347 4088 1517
Size: 1568 32 230 741 4606
Fragments arranged by size:
4606 1568 741 230 32
StyI C'CwwG_G
Cuts at: 513 1065 2022 2064 2295 2304 2541 3342 4047
Size: 552 957 42 231 9 237 801 705
Cuts at: 4047 4284 4616 513
Size: 237 332 3074
Fragments arranged by size:
3074 957 801 705 552 332 237 237
231 42 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2303 5204 5221 5374 5559 6004 325
Size: 1255 723 2901 17 153 185 445 1498
Fragments arranged by size:
2901 1498 1255 723 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3363 3525 3824
Size: 274 80 33 51 1443 1386 162 299
Cuts at: 3824 5423 7135 96
Size: 1599 1712 138
Fragments arranged by size:
1712 1599 1443 1386 299 274 162 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7137 7169 7137
Size: 32 7145
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7177
XbaI T'CTAG_A
Cuts at: 4513 4513
Size: 7177
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2590 3775 4177 4291 2590
Size: 1185 402 114 5476
Fragments arranged by size:
5476 1185 402 114
XhoI C'TCGA_G
Cuts at: 1943 4082 1943
Size: 2139 5038
XmaI C'CCGG_G
Cuts at: 1870 4502 1870
Size: 2632 4545
XmnI GAAnn'nnTTC
Cuts at: 3431 5306 3431
Size: 1875 5302
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4985 276
Size: 53 83 186 936 3451 2468
Fragments arranged by size:
3451 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I FalI FseI FspAI KasI NarI NruI
NsiI PacI PpuMI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI