Restriction map for
pFN21ASDA1138
(Circular) (Six-base) MAPSORT of: pFN21ASDA1138.seq Check: 9421 from: 1 to: 8392
pFN21ASDA1138 8392 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2839 3184 2839
Size: 345 8047
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5397 6202 278
Size: 53 83 186 936 3861 805 2468
Fragments arranged by size:
3861 2468 936 805 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5268 5735 1937
Size: 3331 467 4594
Fragments arranged by size:
4594 3331 467
Acc65I G'GTAC_C
Cuts at: 5713 5713
Size: 8392
AclI AA'CG_TT
Cuts at: 1563 6519 6892 1563
Size: 4956 373 3063
Fragments arranged by size:
4956 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3575 4959 5495 6455 7839 8107 1632
Size: 1943 1384 536 960 1384 268 1917
Fragments arranged by size:
1943 1917 1384 1384 960 536 268
AfeI AGC'GCT
Cuts at: 4481 4654 5425 4481
Size: 173 771 7448
Fragments arranged by size:
7448 771 173
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8195
AflIII A'CryG_T
Cuts at: 1203 5064 5116 5502 7291 8268 1203
Size: 3861 52 386 1789 977 1327
Fragments arranged by size:
3861 1789 1327 977 386 52
AgeI A'CCGG_T
Cuts at: 7223 7223
Size: 8392
AhdI GACnn_n'nnGTC
Cuts at: 7121 7121
Size: 8392
AleI CACnn'nnGTG
Cuts at: 1166 3347 5276 5300 5495 5507 1166
Size: 2181 1929 24 195 12 4051
Fragments arranged by size:
4051 2181 1929 195 24 12
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8392
AlwNI CAG_nnn'CTG
Cuts at: 4330 4938 6135 7707 4330
Size: 608 1197 1572 5015
Fragments arranged by size:
5015 1572 1197 608
ApaI G_GGCC'C
Cuts at: 2091 2131 2138 2441 3172 3202 3275 3568 4117
Size: 40 7 303 731 30 73 293 549
Cuts at: 4117 2091
Size: 6366
Fragments arranged by size:
6366 731 549 303 293 73 40 30
7
ApaLI G'TGCA_C
Cuts at: 5279 6449 7605 5279
Size: 1170 1156 6066
Fragments arranged by size:
6066 1170 1156
ApoI r'AATT_y
Cuts at: 1493 5701 5927 6079 8188 1493
Size: 4208 226 152 2109 1697
Fragments arranged by size:
4208 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2142 2142
Size: 8392
AseI AT'TA_AT
Cuts at: 160 1032 6946 160
Size: 872 5914 1606
Fragments arranged by size:
5914 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8392
AvaI C'yCGr_G
Cuts at: 1870 1943 2081 2130 2772 2799 3992 4048 4767
Size: 73 138 49 642 27 1193 56 719
Cuts at: 4767 4877 5194 5717 1870
Size: 110 317 523 4545
Fragments arranged by size:
4545 1193 719 642 523 317 138 110
73 56 49 27
BamHI G'GATC_C
Cuts at: 5722 6089 5722
Size: 367 8025
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2077 2118 2164 2240 2404
Size: 325 217 627 290 41 46 76 164
Cuts at: 2404 2612 2751 3530 3625 4066 4262 4952 5630
Size: 208 139 779 95 441 196 690 678
Cuts at: 5630 5713 7168 618
Size: 83 1455 1842
Fragments arranged by size:
1842 1455 779 690 678 627 441 325
290 217 208 196 164 139 95 83
76 46 41
BanII G_rGCy'C
Cuts at: 1391 2091 2131 2138 2441 2807 2823 2925 3172
Size: 700 40 7 303 366 16 102 247
Cuts at: 3172 3202 3275 3401 3568 4047 4117 5178 5711
Size: 30 73 126 167 479 70 1061 533
Cuts at: 5711 1391
Size: 4072
Fragments arranged by size:
4072 1061 700 533 479 366 303 247
167 126 102 73 70 40 30 16
7
BbeI G_GCGC'C
Cuts at: 2081 2168 2408 3534 5634 2081
Size: 87 240 1126 2100 4839
Fragments arranged by size:
4839 2100 1126 240 87
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5165 5673 928
Size: 971 3266 508 3647
Fragments arranged by size:
3647 3266 971 508
BbvCI CC'TCA_GC
Cuts at: 2756 3659 4667 4902 2756
Size: 903 1008 235 6246
Fragments arranged by size:
6246 1008 903 235
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4129 4158 4163 4192 6583 6617 1903
Size: 34 2192 29 5 29 2391 34 3678
Fragments arranged by size:
3678 2391 2192 34 34 29 29 5
BciVI GTATCCnnnnn_n'
Cuts at: 6284 7494 6284
Size: 1210 7182
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8392
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2086 2095 2309
Size: 107 122 71 631 605 414 9 214
Cuts at: 2309 2653 3379 3388 3727 4121 4166 4950 5148
Size: 344 726 9 339 394 45 784 198
Cuts at: 5148 7003 136
Size: 1855 1525
Fragments arranged by size:
1855 1525 784 726 631 605 414 394
344 339 214 198 122 107 71 45
9 9
BglII A'GATC_T
Cuts at: 8387 8387
Size: 8392
BlpI GC'TnA_GC
Cuts at: 4748 5411 5809 4748
Size: 663 398 7331
Fragments arranged by size:
7331 663 398
Bme1580I G_kGCm'C
Cuts at: 1790 2091 2131 2138 2441 2617 3172 3202 3275
Size: 301 40 7 303 176 555 30 73
Cuts at: 3275 3568 4071 4117 4662 5283 5610 6453 7609
Size: 293 503 46 545 621 327 843 1156
Cuts at: 7609 1790
Size: 2573
Fragments arranged by size:
2573 1156 843 621 555 545 503 327
303 301 293 176 73 46 40 30
7
BmgBI CAC'GTC
Cuts at: 1318 3058 5278 5505 1318
Size: 1740 2220 227 4205
Fragments arranged by size:
4205 2220 1740 227
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2667 5023 5053
Size: 416 53 83 399 24 1250 2356 30
Cuts at: 5053 6123 7081 442
Size: 1070 958 1753
Fragments arranged by size:
2356 1753 1250 1070 958 416 399 83
53 30 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8392
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2398 3550 2398
Size: 1152 7240
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2300 2354 2381 2683 2738 3023 3053 3437
Size: 1061 54 27 302 55 285 30 384
Cuts at: 3437 3673 3778 3848 5264 5474 5599 7052 1239
Size: 236 105 70 1416 210 125 1453 2579
Fragments arranged by size:
2579 1453 1416 1061 384 302 285 236
210 125 105 70 55 54 30 27
Bpu10I CC'TnA_GC
Cuts at: 2205 2756 3569 3659 4311 4386 4634 4667 4902
Size: 551 813 90 652 75 248 33 235
Cuts at: 4902 2205
Size: 5695
Fragments arranged by size:
5695 813 652 551 248 235 90 75
33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3716 4496 4610 4823 5165 6517 7382 7680 3716
Size: 780 114 213 342 1352 865 298 4428
Fragments arranged by size:
4428 1352 865 780 342 298 213 114
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3023 5048 7055 882
Size: 617 1524 2025 2007 2219
Fragments arranged by size:
2219 2025 2007 1524 617
BsaAI yAC'GTr
Cuts at: 493 1204 2686 5119 5284 5689 6183 493
Size: 711 1482 2433 165 405 494 2702
Fragments arranged by size:
2702 2433 1482 711 494 405 165
BsaBI GATnn'nnATC
Cuts at: 8386 8386
Size: 8392
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2078 2165 2405 2570
Size: 53 83 186 936 545 87 240 165
Cuts at: 2570 3531 3543 4358 5394 5625 5631 5670 6199
Size: 961 12 815 1036 231 6 39 529
Cuts at: 6199 6581 7216 275
Size: 382 635 1451
Fragments arranged by size:
1451 1036 961 936 815 635 545 529
382 240 231 186 165 87 83 53
39 12 6
BsaWI w'CCGG_w
Cuts at: 6825 7223 7497 7644 8055 6825
Size: 398 274 147 411 7162
Fragments arranged by size:
7162 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2717 2747 2818 2848 3659
Size: 30 165 30 1314 30 71 30 811
Cuts at: 3659 3671 3689 3701 3833 3863 5045 5075 1178
Size: 12 18 12 132 30 1182 30 4495
Fragments arranged by size:
4495 1314 1182 811 165 132 71 30
30 30 30 30 30 18 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2273 3757 3760 3763 3766 3769 3772 3775
Size: 1087 1484 3 3 3 3 3 3
Cuts at: 3775 3878 3971 4018 4066 4228 4517 4574 4919
Size: 103 93 47 48 162 289 57 345
Cuts at: 4919 5012 1186
Size: 93 4566
Fragments arranged by size:
4566 1484 1087 345 289 162 103 93
93 57 48 47 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 2182 3079 3097 3182 3653 4116 4586 4649 5049
Size: 897 18 85 471 463 470 63 400
Cuts at: 5049 7595 8091 2182
Size: 2546 496 2483
Fragments arranged by size:
2546 2483 897 496 471 470 463 400
85 63 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3068 5141 1163
Size: 573 1332 2073 4414
Fragments arranged by size:
4414 2073 1332 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3045 3311 3395 3422 3429 5842
Size: 275 186 1044 266 84 27 7 2413
Cuts at: 5842 6603 7631 1540
Size: 761 1028 2301
Fragments arranged by size:
2413 2301 1044 1028 761 275 266 186
84 27 7
BsiHKAI G_wGCw'C
Cuts at: 2379 3476 5178 5283 5514 6453 6538 7609 2379
Size: 1097 1702 105 231 939 85 1071 3162
Fragments arranged by size:
3162 1702 1097 1071 939 231 105 85
BsmI GAATG_Cn'
Cuts at: 5903 5996 5903
Size: 93 8299
BsmBI CGTCTCn'nnnn_
Cuts at: 2357 3032 2357
Size: 675 7717
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2131 2138 2379 2441 2617 2807
Size: 399 301 40 7 241 62 176 190
Cuts at: 2807 2823 2925 3172 3202 3275 3401 3476 3568
Size: 16 102 247 30 73 126 75 92
Cuts at: 3568 4047 4071 4117 4662 5178 5283 5514 5610
Size: 479 24 46 545 516 105 231 96
Cuts at: 5610 5711 6453 6538 7609 1391
Size: 101 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 545 516 479 399 301
247 241 231 190 176 126 105 102
101 96 92 85 75 73 62 46
40 30 24 16 7
BspEI T'CCGG_A
Cuts at: 8055 8055
Size: 8392
BspHI T'CATG_A
Cuts at: 6281 8011 6281
Size: 1730 6662
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2839 3184 4116 4710 4827 4946 5747 844
Size: 1995 345 932 594 117 119 801 3489
Fragments arranged by size:
3489 1995 932 801 594 345 119 117
BsrBI CCG'CTC
Cuts at: 1394 1823 2382 2782 3307 3505 3516 4873 6279
Size: 429 559 400 525 198 11 1357 1406
Cuts at: 6279 1394
Size: 3507
Fragments arranged by size:
3507 1406 1357 559 525 429 400 198
11
BsrDI GCAATG_nn'
Cuts at: 65 1248 6887 7061 65
Size: 1183 5639 174 1396
Fragments arranged by size:
5639 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2094 2510 3378 3421 3428 3918 5084
Size: 110 284 416 868 43 7 490 1166
Cuts at: 5084 5627 7036 7223 1700
Size: 543 1409 187 2869
Fragments arranged by size:
2869 1409 1166 868 543 490 416 284
187 110 43 7
BsrGI T'GTAC_A
Cuts at: 96 4797 96
Size: 4701 3691
BssHII G'CGCG_C
Cuts at: 1925 2142 1925
Size: 217 8175
BssSI C'ACGA_G
Cuts at: 4700 4964 6452 7464 4700
Size: 264 1488 1012 5628
Fragments arranged by size:
5628 1488 1012 264
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4938 5119 794
Size: 4144 181 4067
Fragments arranged by size:
4144 4067 181
BstBI TT'CG_AA
Cuts at: 2007 7196 2007
Size: 5189 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8392
BstXI CCAn_nnnn'nTGG
Cuts at: 2530 2716 3349 2530
Size: 186 633 7573
Fragments arranged by size:
7573 633 186
BstYI r'GATC_y
Cuts at: 1972 3876 4008 5199 5722 6089 6474 6491 7943
Size: 1904 132 1191 523 367 385 17 1452
Cuts at: 7943 8029 8041 8387 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1904 1452 1191 523 385 367 346
132 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3358 4808 3358
Size: 1450 6942
BtgI C'CryG_G
Cuts at: 513 1065 2122 2410 2488 2809 2904 2928 3858
Size: 552 1057 288 78 321 95 24 930
Cuts at: 3858 4062 5493 513
Size: 204 1431 3412
Fragments arranged by size:
3412 1431 1057 930 552 321 288 204
95 78 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2883 487
Size: 683 56 1657 5996
Fragments arranged by size:
5996 1657 683 56
BtsI GCAGTG_nn'
Cuts at: 2326 2821 3056 5908 6702 6722 2326
Size: 495 235 2852 794 20 3996
Fragments arranged by size:
3996 2852 794 495 235 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8392
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4660 4695 529
Size: 35 4096 35 4226
Fragments arranged by size:
4226 4096 35 35
DraI TTT'AAA
Cuts at: 4422 5696 4422
Size: 1274 7118
DraIII CAC_nnn'GTG
Cuts at: 4705 5647 4705
Size: 942 7450
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5378 7399 809
Size: 507 4062 2021 1802
Fragments arranged by size:
4062 2021 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2532 2931 3042
Size: 54 1425 50 275 18 702 399 111
Cuts at: 3042 3308 3339 3351 3380 3392 3426 4281 4321
Size: 266 31 12 29 12 34 855 40
Cuts at: 4321 4611 4681 4725 5040 5128 5565 5839 6728
Size: 290 70 44 315 88 437 274 889
Cuts at: 6728 8357 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 855 702 437 399 315
290 275 274 266 111 88 70 54
50 44 43 40 34 31 29 18
12 12
EagI C'GGCC_G
Cuts at: 1537 1812 3042 3308 3392 3426 5839 1537
Size: 275 1230 266 84 34 2413 4090
Fragments arranged by size:
4090 2413 1230 275 266 84 34
EarI CTCTTCn'nnn_
Cuts at: 1353 3464 3939 3944 3995 4205 4904 4930 4978
Size: 2111 475 5 51 210 699 26 48
Cuts at: 4978 5519 6322 1353
Size: 541 803 3423
Fragments arranged by size:
3423 2111 803 699 541 475 210 51
48 26 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2172 2374 3821 4186 4463 6979
Size: 1064 138 583 202 1447 365 277 2516
Cuts at: 6979 7353 7499 387
Size: 374 146 1280
Fragments arranged by size:
2516 1447 1280 1064 583 374 365 277
202 146 138
EcoICRI GAG'CTC
Cuts at: 5176 5176
Size: 8392
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2300 2354 2381 2683 2738 3023 3053
Size: 393 668 54 27 302 55 285 30
Cuts at: 3053 3437 3575 3673 3778 3848 4959 5264 5474
Size: 384 138 98 105 70 1111 305 210
Cuts at: 5474 5495 5599 6455 7052 7839 8107 1239
Size: 21 104 856 597 787 268 1524
Fragments arranged by size:
1524 1111 856 787 668 597 393 384
305 302 285 268 210 138 105 104
98 70 55 54 30 27 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2255 3830 4314 2255
Size: 1575 484 6333
Fragments arranged by size:
6333 1575 484
EcoO109I rG'GnC_Cy
Cuts at: 2087 2088 2127 2134 2135 2392 2998 3064 3168
Size: 1 39 7 1 257 606 66 104
Cuts at: 3168 3169 3198 3271 3481 3565 4058 4153 4272
Size: 1 29 73 210 84 493 95 119
Cuts at: 4272 4639 4777 4935 2087
Size: 367 138 158 5544
Fragments arranged by size:
5544 606 493 367 257 210 158 138
119 104 95 84 73 66 39 29
7 1 1 1
EcoRI G'AATT_C
Cuts at: 5701 6079 5701
Size: 378 8014
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4756 4756
Size: 8392
FspI TGC'GCA
Cuts at: 2318 6898 2318
Size: 4580 3812
HaeII r_GCGC'y
Cuts at: 1678 2081 2168 2347 2408 3534 4483 4656 5143
Size: 403 87 179 61 1126 949 173 487
Cuts at: 5143 5427 5634 7539 1678
Size: 284 207 1905 2531
Fragments arranged by size:
2531 1905 1126 949 487 403 284 207
179 173 87 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2717 2749 3659 3665 3671
Size: 32 135 32 1145 32 910 6 6
Cuts at: 3671 3691 3697 3703 3833 3865 4042 4074 5571
Size: 20 6 6 130 32 177 32 1497
Cuts at: 5571 5603 7033 7065 7107 7139 1373
Size: 32 1430 32 42 32 2626
Fragments arranged by size:
2626 1497 1430 1145 910 177 135 130
42 32 32 32 32 32 32 32
32 20 6 6 6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2296 5269 5736 5982 669
Size: 988 281 358 2973 467 246 3079
Fragments arranged by size:
3079 2973 988 467 358 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8392
HpaI GTT'AAC
Cuts at: 5982 5982
Size: 8392
KasI G'GCGC_C
Cuts at: 2077 2164 2404 3530 5630 2077
Size: 87 240 1126 2100 4839
Fragments arranged by size:
4839 2100 1126 240 87
KpnI G_GTAC'C
Cuts at: 5717 5717
Size: 8392
MfeI C'AATT_G
Cuts at: 5991 5991
Size: 8392
MluI A'CGCG_T
Cuts at: 8268 8268
Size: 8392
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2265 2760 3702 3876 4326 4551 7506 7690
Size: 960 495 942 174 450 225 2955 184
Cuts at: 7690 1305
Size: 2007
Fragments arranged by size:
2955 2007 960 942 495 450 225 184
174
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3353 4323 10
Size: 54 1425 343 1521 970 4079
Fragments arranged by size:
4079 1521 1425 970 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3347 5276 5300 5495 5507 6350 6709
Size: 648 2181 1929 24 195 12 843 359
Cuts at: 6709 6868 518
Size: 159 2042
Fragments arranged by size:
2181 2042 1929 843 648 359 195 159
24 12
MspA1I CmG'CkG
Cuts at: 1606 2124 2341 2412 2811 3186 3457 4064 4287
Size: 518 217 71 399 375 271 607 223
Cuts at: 4287 4330 4901 4947 4983 5808 6485 7633 7878
Size: 43 571 46 36 825 677 1148 245
Cuts at: 7878 1606
Size: 2120
Fragments arranged by size:
2120 1148 825 677 607 571 518 399
375 271 245 223 217 71 46 43
36
NaeI GCC'GGC
Cuts at: 2096 2512 3430 3920 5086 5629 2096
Size: 416 918 490 1166 543 4859
Fragments arranged by size:
4859 1166 918 543 490 416
NarI GG'CG_CC
Cuts at: 2078 2165 2405 3531 5631 2078
Size: 87 240 1126 2100 4839
Fragments arranged by size:
4839 2100 1126 240 87
NcoI C'CATG_G
Cuts at: 513 1065 3858 513
Size: 552 2793 5047
Fragments arranged by size:
5047 2793 552
NdeI CA'TA_TG
Cuts at: 387 5236 387
Size: 4849 3543
NgoMIV G'CCGG_C
Cuts at: 2094 2510 3428 3918 5084 5627 2094
Size: 416 918 490 1166 543 4859
Fragments arranged by size:
4859 1166 918 543 490 416
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8392
NotI GC'GGCC_GC
Cuts at: 5839 5839
Size: 8392
NspI r_CATG'y
Cuts at: 1132 1229 5068 5419 5641 5750 7295 1132
Size: 97 3839 351 222 109 1545 2229
Fragments arranged by size:
3839 2229 1545 351 222 109 97
PasI CC'CwG_GG
Cuts at: 1348 2245 3202 1348
Size: 897 957 6538
Fragments arranged by size:
6538 957 897
PciI A'CATG_T
Cuts at: 5064 7291 5064
Size: 2227 6165
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4039 4705 5527 1108
Size: 2931 666 822 3973
Fragments arranged by size:
3973 2931 822 666
PfoI T'CCnGG_A
Cuts at: 3702 4989 5589 3702
Size: 1287 600 6505
Fragments arranged by size:
6505 1287 600
PmeI GTTT'AAAC
Cuts at: 5696 5696
Size: 8392
PmlI CAC'GTG
Cuts at: 5119 5284 5119
Size: 165 8227
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6461 6493 7974 8006 6461
Size: 32 1481 32 6847
Fragments arranged by size:
6847 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3064 4058 4272 4777 4935 3064
Size: 994 214 505 158 6521
Fragments arranged by size:
6521 994 505 214 158
PshAI GACnn'nnGTC
Cuts at: 1613 2583 5255 1613
Size: 970 2672 4750
Fragments arranged by size:
4750 2672 970
PsiI TTA'TAA
Cuts at: 5962 5962
Size: 8392
PspOMI G'GGCC_C
Cuts at: 2087 2127 2134 2437 3168 3198 3271 3564 4113
Size: 40 7 303 731 30 73 293 549
Cuts at: 4113 2087
Size: 6366
Fragments arranged by size:
6366 731 549 303 293 73 40 30
7
PstI C_TGCA'G
Cuts at: 830 4152 4252 4934 5744 830
Size: 3322 100 682 810 3478
Fragments arranged by size:
3478 3322 810 682 100
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8392
PvuII CAG'CTG
Cuts at: 3186 3457 4330 4901 3186
Size: 271 873 571 6677
Fragments arranged by size:
6677 873 571 271
SacI G_AGCT'C
Cuts at: 5178 5178
Size: 8392
SacII CC_GC'GG
Cuts at: 2125 2413 2812 4065 2125
Size: 288 399 1253 6452
Fragments arranged by size:
6452 1253 399 288
SalI G'TCGA_C
Cuts at: 1936 5267 5734 1936
Size: 3331 467 4594
Fragments arranged by size:
4594 3331 467
SanDI GG'GwC_CC
Cuts at: 4058 4058
Size: 8392
SapI GCTCTTCn'nnn_
Cuts at: 3464 5519 3464
Size: 2055 6337
SbfI CC_TGCA'GG
Cuts at: 4934 5744 4934
Size: 810 7582
ScaI AGT'ACT
Cuts at: 2561 6640 8352 2561
Size: 4079 1712 2601
Fragments arranged by size:
4079 2601 1712
SexAI A'CCwGG_T
Cuts at: 5336 5643 5336
Size: 307 8085
SfcI C'TryA_G
Cuts at: 826 1045 2473 4148 4248 4930 5740 6875 7556
Size: 219 1428 1675 100 682 810 1135 681
Cuts at: 7556 7747 826
Size: 191 1471
Fragments arranged by size:
1675 1471 1428 1135 810 682 681 219
191 100
SfiI GGCCn_nnn'nGGCC
Cuts at: 2309 3379 2309
Size: 1070 7322
SfoI GGC'GCC
Cuts at: 2079 2166 2406 3532 5632 2079
Size: 87 240 1126 2100 4839
Fragments arranged by size:
4839 2100 1126 240 87
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8392
SgrAI Cr'CCGG_yG
Cuts at: 1700 5627 1700
Size: 3927 4465
SmaI CCC'GGG
Cuts at: 1872 2083 2132 2774 2801 3994 4769 4879 5196
Size: 211 49 642 27 1193 775 110 317
Cuts at: 5196 5719 1872
Size: 523 4545
Fragments arranged by size:
4545 1193 775 642 523 317 211 110
49 27
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3731 4511 4625 4838 5144 6496
Size: 197 926 1788 780 114 213 306 1352
Cuts at: 6496 7397 7659 820
Size: 901 262 1553
Fragments arranged by size:
1788 1553 1352 926 901 780 306 262
213 197 114
SnaBI TAC'GTA
Cuts at: 493 5689 6183 493
Size: 5196 494 2702
Fragments arranged by size:
5196 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8392
SphI G_CATG'C
Cuts at: 1132 5641 5750 1132
Size: 4509 109 3774
Fragments arranged by size:
4509 3774 109
SspI AAT'ATT
Cuts at: 5 52 6316 5
Size: 47 6264 2081
Fragments arranged by size:
6264 2081 47
StuI AGG'CCT
Cuts at: 1517 3373 3737 1517
Size: 1856 364 6172
Fragments arranged by size:
6172 1856 364
StyI C'CwwG_G
Cuts at: 513 1065 2631 3033 3135 3219 3477 3858 3939
Size: 552 1566 402 102 84 258 381 81
Cuts at: 3939 4917 5831 513
Size: 978 914 3074
Fragments arranged by size:
3074 1566 978 914 552 402 381 258
102 84 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2722 4841 4881 5204 6419 6436 6589
Size: 1255 1142 2119 40 323 1215 17 153
Cuts at: 6589 6774 7219 325
Size: 185 445 1498
Fragments arranged by size:
2119 1498 1255 1215 1142 445 323 185
153 40 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2559 4797 5061
Size: 274 80 33 51 1443 582 2238 264
Cuts at: 5061 6638 8350 96
Size: 1577 1712 138
Fragments arranged by size:
2238 1712 1577 1443 582 274 264 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2818 2850 4391 4423 4985 5017 8352 8384 2818
Size: 32 1541 32 562 32 3335 32 2826
Fragments arranged by size:
3335 2826 1541 562 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8392
XbaI T'CTAG_A
Cuts at: 5728 5728
Size: 8392
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2431 2998 4240 5239 2431
Size: 567 1242 999 5584
Fragments arranged by size:
5584 1242 999 567
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8392
XmaI C'CCGG_G
Cuts at: 1870 2081 2130 2772 2799 3992 4767 4877 5194
Size: 211 49 642 27 1193 775 110 317
Cuts at: 5194 5717 1870
Size: 523 4545
Fragments arranged by size:
4545 1193 775 642 523 317 211 110
49 27
XmnI GAAnn'nnTTC
Cuts at: 6521 6521
Size: 8392
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5395 6200 276
Size: 53 83 186 936 3861 805 2468
Fragments arranged by size:
3861 2468 936 805 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FseI
FspAI NruI NsiI PacI PspXI PsrI RsrII SrfI
SwaI