Restriction map for
pFN21ASDA1133
(Circular) (Six-base) MAPSORT of: pFN21ASDA1133.seq Check: 7059 from: 1 to: 7237
pFN21ASDA1133 7237 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2717 3710 4069 2717
Size: 993 359 5885
Fragments arranged by size:
5885 993 359
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5047 278
Size: 53 83 186 936 3511 2468
Fragments arranged by size:
3511 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3429 4580 1937
Size: 1492 1151 4594
Fragments arranged by size:
4594 1492 1151
Acc65I G'GTAC_C
Cuts at: 4558 4558
Size: 7237
AclI AA'CG_TT
Cuts at: 1563 5364 5737 1563
Size: 3801 373 3063
Fragments arranged by size:
3801 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2259 2468 3292 5300 6684 6952 1632
Size: 627 209 824 2008 1384 268 1917
Fragments arranged by size:
2008 1917 1384 824 627 268 209
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7040
AflIII A'CryG_T
Cuts at: 1203 6136 7113 1203
Size: 4933 977 1327
Fragments arranged by size:
4933 1327 977
AgeI A'CCGG_T
Cuts at: 2661 6068 2661
Size: 3407 3830
AhdI GACnn_n'nnGTC
Cuts at: 5966 5966
Size: 7237
AleI CACnn'nnGTG
Cuts at: 1166 3257 1166
Size: 2091 5146
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7237
AlwNI CAG_nnn'CTG
Cuts at: 4110 4980 6552 4110
Size: 870 1572 4795
Fragments arranged by size:
4795 1572 870
ApaI G_GGCC'C
Cuts at: 2566 3206 3671 3756 3933 4189 4381 2566
Size: 640 465 85 177 256 192 5422
Fragments arranged by size:
5422 640 465 256 192 177 85
ApaLI G'TGCA_C
Cuts at: 2837 3260 3629 5294 6450 2837
Size: 423 369 1665 1156 3624
Fragments arranged by size:
3624 1665 1156 423 369
ApoI r'AATT_y
Cuts at: 1493 2467 4546 4772 4924 7033 1493
Size: 974 2079 226 152 2109 1697
Fragments arranged by size:
2109 2079 1697 974 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5791 160
Size: 872 4759 1606
Fragments arranged by size:
4759 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7237
AvaI C'yCGr_G
Cuts at: 1870 1943 2325 3198 3559 3568 3611 3682 3782
Size: 73 382 873 361 9 43 71 100
Cuts at: 3782 4181 4188 4341 4522 4562 1870
Size: 399 7 153 181 40 4545
Fragments arranged by size:
4545 873 399 382 361 181 153 100
73 71 43 40 9 7
AvrII C'CTAG_G
Cuts at: 2140 2140
Size: 7237
BamHI G'GATC_C
Cuts at: 2334 4567 4934 2334
Size: 2233 367 4637
Fragments arranged by size:
4637 2233 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2541 4558 6013 618
Size: 325 217 627 754 2017 1455 1842
Fragments arranged by size:
2017 1842 1455 754 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2237 2566 3206 3533 3560 3602 3621 3671
Size: 846 329 640 327 27 42 19 50
Cuts at: 3671 3756 3783 3870 3933 3950 3972 4189 4360
Size: 85 27 87 63 17 22 217 171
Cuts at: 4360 4381 4556 1391
Size: 21 175 4072
Fragments arranged by size:
4072 846 640 329 327 217 175 171
87 85 63 50 42 27 27 22
21 19 17
BbeI G_GCGC'C
Cuts at: 2545 2545
Size: 7237
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2247 928
Size: 971 348 5918
Fragments arranged by size:
5918 971 348
BbvCI CC'TCA_GC
Cuts at: 3152 3491 3152
Size: 339 6898
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5428 5462 1903
Size: 34 3491 34 3678
Fragments arranged by size:
3678 3491 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4300 5129 6339 4300
Size: 829 1210 5198
Fragments arranged by size:
5198 1210 829
BclI T'GATC_A
Cuts at: 1548 2218 1548
Size: 670 6567
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3548 4481 5848
Size: 107 122 71 631 605 1876 933 1367
Cuts at: 5848 136
Size: 1525
Fragments arranged by size:
1876 1525 1367 933 631 605 122 107
71
BglII A'GATC_T
Cuts at: 4496 7232 4496
Size: 2736 4501
BlpI GC'TnA_GC
Cuts at: 4654 4654
Size: 7237
Bme1580I G_kGCm'C
Cuts at: 1790 2566 2841 3206 3264 3633 3671 3756 3933
Size: 776 275 365 58 369 38 85 177
Cuts at: 3933 4189 4381 4444 5298 6454 1790
Size: 256 192 63 854 1156 2573
Fragments arranged by size:
2573 1156 854 776 369 365 275 256
192 177 85 63 58 38
BmgBI CAC'GTC
Cuts at: 1318 2592 3300 1318
Size: 1274 708 5255
Fragments arranged by size:
5255 1274 708
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2042 3106 3967
Size: 416 53 83 399 24 625 1064 861
Cuts at: 3967 4968 5926 442
Size: 1001 958 1753
Fragments arranged by size:
1753 1064 1001 958 861 625 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7237
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4144 4144
Size: 7237
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2633 3047 3305 3397 3731 3761 4430 5897
Size: 1394 414 258 92 334 30 669 1467
Cuts at: 5897 1239
Size: 2579
Fragments arranged by size:
2579 1467 1394 669 414 334 258 92
30
Bpu10I CC'TnA_GC
Cuts at: 3152 3491 4196 3152
Size: 339 705 6193
Fragments arranged by size:
6193 705 339
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2234 5362 6227 6525 2234
Size: 3128 865 298 2946
Fragments arranged by size:
3128 2946 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2413 2765 3113 5900 882
Size: 617 914 352 348 2787 2219
Fragments arranged by size:
2787 2219 914 617 352 348
BsaAI yAC'GTr
Cuts at: 493 1204 2789 3265 4534 5028 493
Size: 711 1585 476 1269 494 2702
Fragments arranged by size:
2702 1585 1269 711 494 476
BsaBI GATnn'nnATC
Cuts at: 2300 7231 2300
Size: 4931 2306
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2542 5044 5426 6061
Size: 53 83 186 936 1009 2502 382 635
Cuts at: 6061 275
Size: 1451
Fragments arranged by size:
2502 1451 1009 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2661 4402 5670 6068 6342 6489 6900 2661
Size: 1741 1268 398 274 147 411 2998
Fragments arranged by size:
2998 1741 1268 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3161 3191 3395 3425 3520
Size: 30 165 30 1758 30 204 30 95
Cuts at: 3520 3550 3959 3989 4518 4548 1178
Size: 30 409 30 529 30 3867
Fragments arranged by size:
3867 1758 529 409 204 165 95 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2340 2648 3629 3724 4364 4421 1186
Size: 1154 308 981 95 640 57 4002
Fragments arranged by size:
4002 1154 981 640 308 95 57
BseYI C'CCAG_C
Cuts at: 2079 2857 3046 3644 3870 3880 6440 6936 2079
Size: 778 189 598 226 10 2560 496 2380
Fragments arranged by size:
2560 2380 778 598 496 226 189 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2298 2510 3358 3418 4160 1163
Size: 573 562 212 848 60 742 4240
Fragments arranged by size:
4240 848 742 573 562 212 60
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2833 4687 5448 6476 1540
Size: 275 186 832 1854 761 1028 2301
Fragments arranged by size:
2301 1854 1028 832 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2841 3033 3264 3621 3633 3747 5298 5383 6454
Size: 192 231 357 12 114 1551 85 1071
Cuts at: 6454 2841
Size: 3624
Fragments arranged by size:
3624 1551 1071 357 231 192 114 85
12
BsmI GAATG_Cn'
Cuts at: 4748 4841 4748
Size: 93 7144
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2237 2566 2841 3033 3206 3264 3533
Size: 399 447 329 275 192 173 58 269
Cuts at: 3533 3560 3602 3621 3633 3671 3747 3756 3783
Size: 27 42 19 12 38 76 9 27
Cuts at: 3783 3870 3933 3950 3972 4189 4360 4381 4444
Size: 87 63 17 22 217 171 21 63
Cuts at: 4444 4556 5298 5383 6454 1391
Size: 112 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 447 399 329 275 269
217 192 173 171 112 87 85 76
63 63 58 42 38 27 27 22
21 19 17 12 9
BspEI T'CCGG_A
Cuts at: 4402 6900 4402
Size: 2498 4739
BspHI T'CATG_A
Cuts at: 5126 6856 5126
Size: 1730 5507
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2717 3589 3710 4069 4592 844
Size: 1873 872 121 359 523 3489
Fragments arranged by size:
3489 1873 872 523 359 121
BsrBI CCG'CTC
Cuts at: 1394 1823 2248 3011 3228 3291 3330 4354 5124
Size: 429 425 763 217 63 39 1024 770
Cuts at: 5124 1394
Size: 3507
Fragments arranged by size:
3507 1024 770 763 429 425 217 63
39
BsrDI GCAATG_nn'
Cuts at: 65 1248 5732 5906 65
Size: 1183 4484 174 1396
Fragments arranged by size:
4484 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2257 2661 2815 2922 5881 6068 1700
Size: 110 447 404 154 107 2959 187 2869
Fragments arranged by size:
2959 2869 447 404 187 154 110 107
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7237
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7237
BssSI C'ACGA_G
Cuts at: 3836 5297 6309 3836
Size: 1461 1012 4764
Fragments arranged by size:
4764 1461 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4110 794
Size: 3316 3921
BstBI TT'CG_AA
Cuts at: 2007 6041 2007
Size: 4034 3203
BstEII G'GTnAC_C
Cuts at: 1184 2806 1184
Size: 1622 5615
BstXI CCAn_nnnn'nTGG
Cuts at: 2611 2611
Size: 7237
BstYI r'GATC_y
Cuts at: 1972 2334 4496 4567 4934 5319 5336 6788 6874
Size: 362 2162 71 367 385 17 1452 86
Cuts at: 6874 6886 7232 1972
Size: 12 346 1977
Fragments arranged by size:
2162 1977 1452 385 367 362 346 86
71 17 12
Bsu36I CC'TnA_GG
Cuts at: 3812 3812
Size: 7237
BtgI C'CryG_G
Cuts at: 513 1065 2890 2916 3255 4087 4313 513
Size: 552 1825 26 339 832 226 3437
Fragments arranged by size:
3437 1825 832 552 339 226 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6498
Fragments arranged by size:
6498 683 56
BtsI GCAGTG_nn'
Cuts at: 3238 3346 4470 4753 5547 5567 3238
Size: 108 1124 283 794 20 4908
Fragments arranged by size:
4908 1124 794 283 108 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7237
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3058 3093 529
Size: 35 2494 35 4673
Fragments arranged by size:
4673 2494 35 35
DraI TTT'AAA
Cuts at: 4541 4541
Size: 7237
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6244 809
Size: 507 4928 1802
Fragments arranged by size:
4928 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2830 3006 3079
Size: 54 1425 50 275 18 1000 176 73
Cuts at: 3079 3322 3351 3394 3662 4684 5573 7202 8
Size: 243 29 43 268 1022 889 1629 43
Fragments arranged by size:
1629 1425 1022 1000 889 275 268 243
176 73 54 50 43 43 29 18
EagI C'GGCC_G
Cuts at: 1537 1812 2830 4684 1537
Size: 275 1018 1854 4090
Fragments arranged by size:
4090 1854 1018 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2052 3251 4065 4341 5167 1353
Size: 699 1199 814 276 826 3423
Fragments arranged by size:
3423 1199 826 814 699 276
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4110 5824 6198 6344 387
Size: 1064 138 2521 1714 374 146 1280
Fragments arranged by size:
2521 1714 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3619 3619
Size: 7237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2259 2468 2633 3047 3292 3305 3397
Size: 393 627 209 165 414 245 13 92
Cuts at: 3397 3731 3761 4430 5300 5897 6684 6952 1239
Size: 334 30 669 870 597 787 268 1524
Fragments arranged by size:
1524 870 787 669 627 597 414 393
334 268 245 209 165 92 30 13
EcoNI CCTnn'n_nnAGG
Cuts at: 2144 3061 3941 2144
Size: 917 880 5440
Fragments arranged by size:
5440 917 880
EcoO109I rG'GnC_Cy
Cuts at: 2562 2563 3148 3202 3578 3668 3752 3819 3929
Size: 1 585 54 376 90 84 67 110
Cuts at: 3929 3930 4185 4192 4266 4327 4377 4378 2562
Size: 1 255 7 74 61 50 1 5421
Fragments arranged by size:
5421 585 376 255 110 90 84 74
67 61 54 50 7 1 1 1
EcoRI G'AATT_C
Cuts at: 2467 4546 4924 2467
Size: 2079 378 4780
Fragments arranged by size:
4780 2079 378
FspI TGC'GCA
Cuts at: 5743 5743
Size: 7237
HaeII r_GCGC'y
Cuts at: 1678 2545 3897 6384 1678
Size: 867 1352 2487 2531
Fragments arranged by size:
2531 2487 1352 867
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2319 2351 3161 3193 3520
Size: 32 135 32 747 32 810 32 327
Cuts at: 3520 3552 3680 3712 3830 3862 3959 3991 5878
Size: 32 128 32 118 32 97 32 1887
Cuts at: 5878 5910 5952 5984 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1887 810 747 327 135 128 118
97 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2308 4581 4827 669
Size: 988 281 370 2273 246 3079
Fragments arranged by size:
3079 2273 988 370 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7237
HpaI GTT'AAC
Cuts at: 4827 4827
Size: 7237
KasI G'GCGC_C
Cuts at: 2541 2541
Size: 7237
KpnI G_GTAC'C
Cuts at: 4562 4562
Size: 7237
MfeI C'AATT_G
Cuts at: 4836 4836
Size: 7237
MluI A'CGCG_T
Cuts at: 7113 7113
Size: 7237
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2619 6351 6535 1305
Size: 1314 3732 184 2007
Fragments arranged by size:
3732 2007 1314 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5415
Fragments arranged by size:
5415 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3257 3636 5195 5554 5713 518
Size: 648 2091 379 1559 359 159 2042
Fragments arranged by size:
2091 2042 1559 648 379 359 159
MspA1I CmG'CkG
Cuts at: 1606 2167 2224 3018 3312 3905 4089 4107 4653
Size: 561 57 794 294 593 184 18 546
Cuts at: 4653 5330 6478 6723 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 794 677 593 561 546 294
245 184 57 18
NarI GG'CG_CC
Cuts at: 2542 2542
Size: 7237
NcoI C'CATG_G
Cuts at: 513 1065 2916 513
Size: 552 1851 4834
Fragments arranged by size:
4834 1851 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7237
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7237
NotI GC'GGCC_GC
Cuts at: 4684 4684
Size: 7237
NspI r_CATG'y
Cuts at: 1132 1229 4595 6140 1132
Size: 97 3366 1545 2229
Fragments arranged by size:
3366 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3988 1348
Size: 2640 4597
PciI A'CATG_T
Cuts at: 6136 6136
Size: 7237
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3004 1108
Size: 1896 5341
PfoI T'CCnGG_A
Cuts at: 4446 4446
Size: 7237
PmeI GTTT'AAAC
Cuts at: 4541 4541
Size: 7237
PmlI CAC'GTG
Cuts at: 2789 3265 2789
Size: 476 6761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2219 2251 5306 5338 6819 6851 2219
Size: 32 3055 32 1481 32 2605
Fragments arranged by size:
3055 2605 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3578 3819 4192 4266 3578
Size: 241 373 74 6549
Fragments arranged by size:
6549 373 241 74
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7237
PsiI TTA'TAA
Cuts at: 4807 4807
Size: 7237
PspOMI G'GGCC_C
Cuts at: 2562 3202 3667 3752 3929 4185 4377 2562
Size: 640 465 85 177 256 192 5422
Fragments arranged by size:
5422 640 465 256 192 177 85
PspXI vC'TCGA_Gb
Cuts at: 2325 3782 2325
Size: 1457 5780
PstI C_TGCA'G
Cuts at: 830 2182 2700 3707 4489 4589 830
Size: 1352 518 1007 782 100 3478
Fragments arranged by size:
3478 1352 1007 782 518 100
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7237
PvuII CAG'CTG
Cuts at: 2224 3312 3905 2224
Size: 1088 593 5556
Fragments arranged by size:
5556 1088 593
SacI G_AGCT'C
Cuts at: 3621 3621
Size: 7237
SacII CC_GC'GG
Cuts at: 4090 4090
Size: 7237
SalI G'TCGA_C
Cuts at: 1936 4579 1936
Size: 2643 4594
SanDI GG'GwC_CC
Cuts at: 4266 4266
Size: 7237
SapI GCTCTTCn'nnn_
Cuts at: 3251 3251
Size: 7237
SbfI CC_TGCA'GG
Cuts at: 4589 4589
Size: 7237
ScaI AGT'ACT
Cuts at: 5485 7197 5485
Size: 1712 5525
SfcI C'TryA_G
Cuts at: 826 1045 2178 2696 3703 3721 4485 4585 5720
Size: 219 1133 518 1007 18 764 100 1135
Cuts at: 5720 6401 6592 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1133 1007 764 681 518 219
191 100 18
SfoI GGC'GCC
Cuts at: 2543 2543
Size: 7237
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7237
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7237
SmaI CCC'GGG
Cuts at: 1872 3200 4183 4190 4343 4524 4564 1872
Size: 1328 983 7 153 181 40 4545
Fragments arranged by size:
4545 1328 983 181 153 40 7
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2249 2325 3782 5341 6242 6504
Size: 197 926 306 76 1457 1559 901 262
Cuts at: 6504 820
Size: 1553
Fragments arranged by size:
1559 1553 1457 926 901 306 262 197
76
SnaBI TAC'GTA
Cuts at: 493 4534 5028 493
Size: 4041 494 2702
Fragments arranged by size:
4041 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7237
SphI G_CATG'C
Cuts at: 1132 4595 1132
Size: 3463 3774
SspI AAT'ATT
Cuts at: 5 52 5161 5
Size: 47 5109 2081
Fragments arranged by size:
5109 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7237
StyI C'CwwG_G
Cuts at: 513 1065 2140 2412 2916 3093 3659 4261 4676
Size: 552 1075 272 504 177 566 602 415
Cuts at: 4676 513
Size: 3074
Fragments arranged by size:
3074 1075 602 566 552 504 415 272
177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2043 4507 5264 5281 5434 5619 6064
Size: 1255 463 2464 757 17 153 185 445
Cuts at: 6064 325
Size: 1498
Fragments arranged by size:
2464 1498 1255 757 463 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2161 2619 2654
Size: 274 80 33 51 1443 184 458 35
Cuts at: 2654 4143 5483 7195 96
Size: 1489 1340 1712 138
Fragments arranged by size:
1712 1489 1443 1340 458 274 184 138
80 51 35 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3395 3427 7197 7229 3395
Size: 32 3770 32 3403
Fragments arranged by size:
3770 3403 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2460 1316
Size: 1144 6093
XbaI T'CTAG_A
Cuts at: 2497 4573 2497
Size: 2076 5161
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 2479 3421 3706 2029
Size: 450 942 285 5560
Fragments arranged by size:
5560 942 450 285
XhoI C'TCGA_G
Cuts at: 1943 2325 3782 1943
Size: 382 1457 5398
Fragments arranged by size:
5398 1457 382
XmaI C'CCGG_G
Cuts at: 1870 3198 4181 4188 4341 4522 4562 1870
Size: 1328 983 7 153 181 40 4545
Fragments arranged by size:
4545 1328 983 181 153 40 7
XmnI GAAnn'nnTTC
Cuts at: 5366 5366
Size: 7237
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5045 276
Size: 53 83 186 936 3511 2468
Fragments arranged by size:
3511 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BfrBI BsiWI BsmBI BstZ17I
DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PsrI RsrII SexAI SfiI SrfI SwaI