Restriction map for
pFN21ASDA1099
(Circular) (Six-base) MAPSORT of: pFN21ASDA1099.seq Check: 6361 from: 1 to: 7141
pFN21ASDA1099 7141 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2788 4077 2788
Size: 1289 5852
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4248 4951 278
Size: 53 83 186 936 2712 703 2468
Fragments arranged by size:
2712 2468 936 703 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3966 4484 1937
Size: 2029 518 4594
Fragments arranged by size:
4594 2029 518
Acc65I G'GTAC_C
Cuts at: 2292 4462 2292
Size: 2170 4971
AclI AA'CG_TT
Cuts at: 1563 5268 5641 1563
Size: 3705 373 3063
Fragments arranged by size:
3705 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2437 3525 5204 6588 6856 1632
Size: 805 1088 1679 1384 268 1917
Fragments arranged by size:
1917 1679 1384 1088 805 268
AfeI AGC'GCT
Cuts at: 2078 4352 2078
Size: 2274 4867
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6944
AflIII A'CryG_T
Cuts at: 1203 2651 6040 7017 1203
Size: 1448 3389 977 1327
Fragments arranged by size:
3389 1448 1327 977
AgeI A'CCGG_T
Cuts at: 2289 5972 2289
Size: 3683 3458
AhdI GACnn_n'nnGTC
Cuts at: 2217 3490 5870 2217
Size: 1273 2380 3488
Fragments arranged by size:
3488 2380 1273
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7141
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2820 4196 4337 1184
Size: 1636 1376 141 3988
Fragments arranged by size:
3988 1636 1376 141
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3981 4013 3981
Size: 32 7109
AlwNI CAG_nnn'CTG
Cuts at: 2041 2734 3625 3927 4069 4225 4884 6456 2041
Size: 693 891 302 142 156 659 1572 2726
Fragments arranged by size:
2726 1572 891 693 659 302 156 142
ApaI G_GGCC'C
Cuts at: 2405 2405
Size: 7141
ApaLI G'TGCA_C
Cuts at: 2285 2635 2810 5198 6354 2285
Size: 350 175 2388 1156 3072
Fragments arranged by size:
3072 2388 1156 350 175
ApoI r'AATT_y
Cuts at: 1493 3520 4450 4676 4828 6937 1493
Size: 2027 930 226 152 2109 1697
Fragments arranged by size:
2109 2027 1697 930 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5695 160
Size: 872 4663 1606
Fragments arranged by size:
4663 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7141
AvaI C'yCGr_G
Cuts at: 1870 1943 2581 3255 3840 4131 4254 4466 1870
Size: 73 638 674 585 291 123 212 4545
Fragments arranged by size:
4545 674 638 585 291 212 123 73
BamHI G'GATC_C
Cuts at: 3810 3996 4471 4838 3810
Size: 186 475 367 6113
Fragments arranged by size:
6113 475 367 186
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2292 3827 4420 4462 5917
Size: 325 217 627 505 1535 593 42 1455
Cuts at: 5917 618
Size: 1842
Fragments arranged by size:
1842 1535 1455 627 593 505 325 217
42
BanII G_rGCy'C
Cuts at: 1391 2238 2405 3787 3882 4460 1391
Size: 847 167 1382 95 578 4072
Fragments arranged by size:
4072 1382 847 578 167 95
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2443 2677 928
Size: 971 544 234 5392
Fragments arranged by size:
5392 971 544 234
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2636 2670 5332 5366 1903
Size: 34 699 34 2662 34 3678
Fragments arranged by size:
3678 2662 699 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5033 6243 5033
Size: 1210 5931
BclI T'GATC_A
Cuts at: 1548 2385 1548
Size: 837 6304
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2051 2063 3495
Size: 107 122 71 631 605 379 12 1432
Cuts at: 3495 4295 5752 136
Size: 800 1457 1525
Fragments arranged by size:
1525 1457 1432 800 631 605 379 122
107 71 12
BglII A'GATC_T
Cuts at: 7136 7136
Size: 7141
BlpI GC'TnA_GC
Cuts at: 4558 4558
Size: 7141
Bme1580I G_kGCm'C
Cuts at: 1790 2289 2306 2405 2639 2814 4425 5202 6358
Size: 499 17 99 234 175 1611 777 1156
Cuts at: 6358 1790
Size: 2573
Fragments arranged by size:
2573 1611 1156 777 499 234 175 99
17
BmgBI CAC'GTC
Cuts at: 1318 2170 2652 3264 4321 1318
Size: 852 482 612 1057 4138
Fragments arranged by size:
4138 1057 852 612 482
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3162 3375 3772
Size: 416 53 83 399 24 1745 213 397
Cuts at: 3772 4872 5830 442
Size: 1100 958 1753
Fragments arranged by size:
1753 1745 1100 958 416 399 397 213
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3917 1056
Size: 2861 4280
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2144 3041 3268 3358 3373 4285 5801 1239
Size: 905 897 227 90 15 912 1516 2579
Fragments arranged by size:
2579 1516 912 905 897 227 90 15
Bpu10I CC'TnA_GC
Cuts at: 3805 3805
Size: 7141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5266 6131 6429 5266
Size: 865 298 5978
Fragments arranged by size:
5978 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3740 4142 5804 882
Size: 617 2241 402 1662 2219
Fragments arranged by size:
2241 2219 1662 617 402
BsaAI yAC'GTr
Cuts at: 493 1204 2640 4438 4932 493
Size: 711 1436 1798 494 2702
Fragments arranged by size:
2702 1798 1436 711 494
BsaBI GATnn'nnATC
Cuts at: 7135 7135
Size: 7141
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2598 3594 4245 4948
Size: 53 83 186 936 1065 996 651 703
Cuts at: 4948 5330 5965 275
Size: 382 635 1451
Fragments arranged by size:
1451 1065 996 936 703 651 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2289 2326 3708 3816 4398 5574 5972 6246 6393
Size: 37 1382 108 582 1176 398 274 147
Cuts at: 6393 6804 2289
Size: 411 2626
Fragments arranged by size:
2626 1382 1176 582 411 398 274 147
108 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2090 2120 2402 2432 2843
Size: 30 165 30 687 30 282 30 411
Cuts at: 2843 2873 4230 4260 1178
Size: 30 1357 30 4059
Fragments arranged by size:
4059 1357 687 411 282 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2150 2333 2851 3599 1186
Size: 964 183 518 748 4728
Fragments arranged by size:
4728 964 748 518 183
BseYI C'CCAG_C
Cuts at: 2771 2879 3355 6344 6840 2771
Size: 108 476 2989 496 3072
Fragments arranged by size:
3072 2989 496 476 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4027 1163
Size: 573 2291 4277
Fragments arranged by size:
4277 2291 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3052 4171 4591 5352 6380 1540
Size: 275 186 1051 1119 420 761 1028 2301
Fragments arranged by size:
2301 1119 1051 1028 761 420 275 186
BsiHKAI G_wGCw'C
Cuts at: 2169 2238 2289 2639 2814 3882 4362 5202 5287
Size: 69 51 350 175 1068 480 840 85
Cuts at: 5287 6358 2169
Size: 1071 2952
Fragments arranged by size:
2952 1071 1068 840 480 350 175 85
69 51
BsmI GAATG_Cn'
Cuts at: 3806 4652 4745 3806
Size: 846 93 6202
Fragments arranged by size:
6202 846 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2642 4158 2642
Size: 1516 5625
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2169 2238 2289 2306 2405 2639 2814
Size: 399 379 69 51 17 99 234 175
Cuts at: 2814 3787 3882 4362 4425 4460 5202 5287 6358
Size: 973 95 480 63 35 742 85 1071
Cuts at: 6358 1391
Size: 2174
Fragments arranged by size:
2174 1071 973 742 480 399 379 234
175 99 95 85 69 63 51 35
17
BspEI T'CCGG_A
Cuts at: 2326 3708 6804 2326
Size: 1382 3096 2663
Fragments arranged by size:
3096 2663 1382
BspHI T'CATG_A
Cuts at: 5030 6760 5030
Size: 1730 5411
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2328 2788 4077 4104 4160 4496 844
Size: 1484 460 1289 27 56 336 3489
Fragments arranged by size:
3489 1484 1289 460 336 56 27
BsrBI CCG'CTC
Cuts at: 1394 1823 3048 3589 5028 1394
Size: 429 1225 541 1439 3507
Fragments arranged by size:
3507 1439 1225 541 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5636 5810 65
Size: 1183 4388 174 1396
Fragments arranged by size:
4388 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2140 2289 2961 5785 5972 1700
Size: 110 330 149 672 2824 187 2869
Fragments arranged by size:
2869 2824 672 330 187 149 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7141
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7141
BssSI C'ACGA_G
Cuts at: 2524 5201 6213 2524
Size: 2677 1012 3452
Fragments arranged by size:
3452 2677 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3801 4072 794
Size: 3007 271 3863
Fragments arranged by size:
3863 3007 271
BstBI TT'CG_AA
Cuts at: 2007 5945 2007
Size: 3938 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7141
BstXI CCAn_nnnn'nTGG
Cuts at: 2431 2431
Size: 7141
BstYI r'GATC_y
Cuts at: 1972 2070 2157 3621 3810 3996 4471 4838 5223
Size: 98 87 1464 189 186 475 367 385
Cuts at: 5223 5240 6692 6778 6790 7136 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1464 1452 475 385 367 346 189
186 98 87 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3690 4264 513
Size: 552 2625 574 3390
Fragments arranged by size:
3390 2625 574 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2158 487
Size: 683 56 932 5470
Fragments arranged by size:
5470 932 683 56
BtsI GCAGTG_nn'
Cuts at: 2919 3498 4417 4657 5451 5471 2919
Size: 579 919 240 794 20 4589
Fragments arranged by size:
4589 919 794 579 240 20
ClaI AT'CG_AT
Cuts at: 1554 2592 2907 1554
Size: 1038 315 5788
Fragments arranged by size:
5788 1038 315
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4393 4428 529
Size: 35 3829 35 3242
Fragments arranged by size:
3829 3242 35 35
DraI TTT'AAA
Cuts at: 4445 4445
Size: 7141
DraIII CAC_nnn'GTG
Cuts at: 3138 3736 3138
Size: 598 6543
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3599 6148 809
Size: 507 2283 2549 1802
Fragments arranged by size:
2549 2283 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2262 2508
Size: 54 1425 50 275 18 213 219 246
Cuts at: 2508 3049 3550 3627 3869 4104 4168 4365 4588
Size: 541 501 77 242 235 64 197 223
Cuts at: 4588 5477 7106 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 541 501 275 246 242
235 223 219 213 197 77 64 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3049 4168 4588 1537
Size: 275 1237 1119 420 4090
Fragments arranged by size:
4090 1237 1119 420 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3239 3927 3975 4028 5071 1353
Size: 1886 688 48 53 1043 3423
Fragments arranged by size:
3423 1886 1043 688 53 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3964 5728 6102 6248 387
Size: 1064 138 2375 1764 374 146 1280
Fragments arranged by size:
2375 1764 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2236 3880 2236
Size: 1644 5497
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2144 2437 3041 3268 3358 3373 3525
Size: 393 512 293 604 227 90 15 152
Cuts at: 3525 4285 5204 5801 6588 6856 1239
Size: 760 919 597 787 268 1524
Fragments arranged by size:
1524 919 787 760 604 597 512 393
293 268 227 152 90 15
EcoNI CCTnn'n_nnAGG
Cuts at: 3308 3308
Size: 7141
EcoO109I rG'GnC_Cy
Cuts at: 2401 2402 2744 4093 2401
Size: 1 342 1349 5449
Fragments arranged by size:
5449 1349 342 1
EcoRI G'AATT_C
Cuts at: 4450 4828 4450
Size: 378 6763
FspI TGC'GCA
Cuts at: 3274 5647 3274
Size: 2373 4768
HaeII r_GCGC'y
Cuts at: 1678 2080 2962 4181 4354 6288 1678
Size: 402 882 1219 173 1934 2531
Fragments arranged by size:
2531 1934 1219 882 402 173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2203 2235 2843 2875 3148
Size: 32 135 32 631 32 608 32 273
Cuts at: 3148 3180 3476 3508 3739 3771 4230 4262 5782
Size: 32 296 32 231 32 459 32 1520
Cuts at: 5782 5814 5856 5888 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1520 631 608 459 296 273 231
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4485 4731 669
Size: 988 281 2547 246 3079
Fragments arranged by size:
3079 2547 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7141
HpaI GTT'AAC
Cuts at: 4731 4731
Size: 7141
KpnI G_GTAC'C
Cuts at: 2296 4466 2296
Size: 2170 4971
MfeI C'AATT_G
Cuts at: 4740 4740
Size: 7141
MluI A'CGCG_T
Cuts at: 7017 7017
Size: 7141
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2643 2991 6255 6439 1305
Size: 1338 348 3264 184 2007
Fragments arranged by size:
3264 2007 1338 348 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2045 2264 2510 3552 3629
Size: 54 1425 343 213 219 246 1042 77
Cuts at: 3629 3871 4367 10
Size: 242 496 2784
Fragments arranged by size:
2784 1425 1042 496 343 246 242 219
213 77 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2429 3887 4319 5099 5458 5617 518
Size: 648 1263 1458 432 780 359 159 2042
Fragments arranged by size:
2042 1458 1263 780 648 432 359 159
MspA1I CmG'CkG
Cuts at: 1606 2041 2268 2734 4036 4557 5234 6382 6627
Size: 435 227 466 1302 521 677 1148 245
Cuts at: 6627 1606
Size: 2120
Fragments arranged by size:
2120 1302 1148 677 521 466 435 245
227
NcoI C'CATG_G
Cuts at: 513 1065 3690 513
Size: 552 2625 3964
Fragments arranged by size:
3964 2625 552
NdeI CA'TA_TG
Cuts at: 387 2308 387
Size: 1921 5220
NheI G'CTAG_C
Cuts at: 1052 3913 1052
Size: 2861 4280
NotI GC'GGCC_GC
Cuts at: 4588 4588
Size: 7141
NspI r_CATG'y
Cuts at: 1132 1229 3079 3187 3718 4499 6044 1132
Size: 97 1850 108 531 781 1545 2229
Fragments arranged by size:
2229 1850 1545 781 531 108 97
PasI CC'CwG_GG
Cuts at: 1348 3239 4057 1348
Size: 1891 818 4432
Fragments arranged by size:
4432 1891 818
PciI A'CATG_T
Cuts at: 6040 6040
Size: 7141
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3625 3736 1108
Size: 2517 111 4513
Fragments arranged by size:
4513 2517 111
PfoI T'CCnGG_A
Cuts at: 2160 2229 2313 2688 4382 2160
Size: 69 84 375 1694 4919
Fragments arranged by size:
4919 1694 375 84 69
PmeI GTTT'AAAC
Cuts at: 4445 4445
Size: 7141
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5210 5242 6723 6755 5210
Size: 32 1481 32 5596
Fragments arranged by size:
5596 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2744 4093 2744
Size: 1349 5792
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7141
PsiI TTA'TAA
Cuts at: 4711 4711
Size: 7141
PspOMI G'GGCC_C
Cuts at: 2401 2401
Size: 7141
PstI C_TGCA'G
Cuts at: 830 2151 3639 3702 4332 4493 830
Size: 1321 1488 63 630 161 3478
Fragments arranged by size:
3478 1488 1321 630 161 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7141
PvuII CAG'CTG
Cuts at: 2041 2734 2041
Size: 693 6448
SacI G_AGCT'C
Cuts at: 2238 3882 2238
Size: 1644 5497
SalI G'TCGA_C
Cuts at: 1936 4483 1936
Size: 2547 4594
SapI GCTCTTCn'nnn_
Cuts at: 4028 4028
Size: 7141
SbfI CC_TGCA'GG
Cuts at: 4493 4493
Size: 7141
ScaI AGT'ACT
Cuts at: 5389 7101 5389
Size: 1712 5429
SfcI C'TryA_G
Cuts at: 826 1045 2147 2497 3635 3698 4328 4489 5624
Size: 219 1102 350 1138 63 630 161 1135
Cuts at: 5624 6305 6496 826
Size: 681 191 1471
Fragments arranged by size:
1471 1138 1135 1102 681 630 350 219
191 161 63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7141
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7141
SmaI CCC'GGG
Cuts at: 1872 4133 4468 1872
Size: 2261 335 4545
Fragments arranged by size:
4545 2261 335
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5245 6146 6408 820
Size: 197 926 3302 901 262 1553
Fragments arranged by size:
3302 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4438 4932 493
Size: 3945 494 2702
Fragments arranged by size:
3945 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7141
SphI G_CATG'C
Cuts at: 1132 3079 4499 1132
Size: 1947 1420 3774
Fragments arranged by size:
3774 1947 1420
SspI AAT'ATT
Cuts at: 5 52 5065 5
Size: 47 5013 2081
Fragments arranged by size:
5013 2081 47
StuI AGG'CCT
Cuts at: 1517 3018 4298 1517
Size: 1501 1280 4360
Fragments arranged by size:
4360 1501 1280
StyI C'CwwG_G
Cuts at: 513 1065 3309 3690 4580 513
Size: 552 2244 381 890 3074
Fragments arranged by size:
3074 2244 890 552 381
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2077 2419 3292 3347 3352 3845 5168
Size: 1255 497 342 873 55 5 493 1323
Cuts at: 5168 5185 5338 5523 5968 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1323 1255 873 497 493 445 342
185 153 55 17 5
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5387 7099 96
Size: 274 80 33 51 1443 3410 1712 138
Fragments arranged by size:
3410 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7101 7133 7101
Size: 32 7109
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7141
XbaI T'CTAG_A
Cuts at: 4477 4477
Size: 7141
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2828 3775 3821 4114 2828
Size: 947 46 293 5855
Fragments arranged by size:
5855 947 293 46
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7141
XmaI C'CCGG_G
Cuts at: 1870 4131 4466 1870
Size: 2261 335 4545
Fragments arranged by size:
4545 2261 335
XmnI GAAnn'nnTTC
Cuts at: 4022 5270 4022
Size: 1248 5893
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4246 4949 276
Size: 53 83 186 936 2712 703 2468
Fragments arranged by size:
2712 2468 936 703 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI
BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI