Restriction map for
pFN21ASDA1098
(Circular) (Six-base) MAPSORT of: pFN21ASDA1098.seq Check: 8881 from: 1 to: 6208
pFN21ASDA1098 6208 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2167 2198 3007 2167
Size: 31 809 5368
Fragments arranged by size:
5368 809 31
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4018 278
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3551 1937
Size: 1614 4594
Acc65I G'GTAC_C
Cuts at: 3529 3529
Size: 6208
AclI AA'CG_TT
Cuts at: 1563 4335 4708 1563
Size: 2772 373 3063
Fragments arranged by size:
3063 2772 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2917 4271 5655 5923 1632
Size: 1285 1354 1384 268 1917
Fragments arranged by size:
1917 1384 1354 1285 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6011
AflIII A'CryG_T
Cuts at: 1203 2040 3142 5107 6084 1203
Size: 837 1102 1965 977 1327
Fragments arranged by size:
1965 1327 1102 977 837
AgeI A'CCGG_T
Cuts at: 5039 5039
Size: 6208
AhdI GACnn_n'nnGTC
Cuts at: 2212 4937 2212
Size: 2725 3483
AleI CACnn'nnGTG
Cuts at: 1166 3477 1166
Size: 2311 3897
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6208
AlwNI CAG_nnn'CTG
Cuts at: 3951 5523 3951
Size: 1572 4636
ApaLI G'TGCA_C
Cuts at: 3426 4265 5421 3426
Size: 839 1156 4213
Fragments arranged by size:
4213 1156 839
ApoI r'AATT_y
Cuts at: 1493 3517 3743 3895 6004 1493
Size: 2024 226 152 2109 1697
Fragments arranged by size:
2109 2024 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4762 160
Size: 872 3730 1606
Fragments arranged by size:
3730 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
AvaI C'yCGr_G
Cuts at: 1870 1943 2048 2360 3157 3202 3533 1870
Size: 73 105 312 797 45 331 4545
Fragments arranged by size:
4545 797 331 312 105 73 45
BamHI G'GATC_C
Cuts at: 2580 3538 3905 2580
Size: 958 367 4883
Fragments arranged by size:
4883 958 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2415 3046 3434 3529 4984
Size: 325 217 627 628 631 388 95 1455
Cuts at: 4984 618
Size: 1842
Fragments arranged by size:
1842 1455 631 628 627 388 325 217
95
BanII G_rGCy'C
Cuts at: 1391 2879 3065 3086 3527 1391
Size: 1488 186 21 441 4072
Fragments arranged by size:
4072 1488 441 186 21
BbeI G_GCGC'C
Cuts at: 3050 3438 3050
Size: 388 5820
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3064 928
Size: 971 1165 4072
Fragments arranged by size:
4072 1165 971
BbvCI CC'TCA_GC
Cuts at: 3165 3165
Size: 6208
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2796 2830 2836 2870 4399 4433 1903
Size: 34 859 34 6 34 1529 34 3678
Fragments arranged by size:
3678 1529 859 34 34 34 34 6
BciVI GTATCCnnnnn_n'
Cuts at: 4100 5310 4100
Size: 1210 4998
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6208
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2510 3060 4819
Size: 107 122 71 631 605 838 550 1759
Cuts at: 4819 136
Size: 1525
Fragments arranged by size:
1759 1525 838 631 605 550 122 107
71
BglII A'GATC_T
Cuts at: 6203 6203
Size: 6208
BlpI GC'TnA_GC
Cuts at: 2345 3625 2345
Size: 1280 4928
Bme1580I G_kGCm'C
Cuts at: 1790 2418 3430 4269 5425 1790
Size: 628 1012 839 1156 2573
Fragments arranged by size:
2573 1156 1012 839 628
BmgBI CAC'GTC
Cuts at: 1318 2041 3273 1318
Size: 723 1232 4253
Fragments arranged by size:
4253 1232 723
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2189 3939 4897
Size: 416 53 83 399 24 772 1750 958
Cuts at: 4897 442
Size: 1753
Fragments arranged by size:
1753 1750 958 772 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6208
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3190 3190
Size: 6208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3145 3338 3429 4868 1239
Size: 1906 193 91 1439 2579
Fragments arranged by size:
2579 1906 1439 193 91
Bpu10I CC'TnA_GC
Cuts at: 3165 3165
Size: 6208
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2635 4333 5198 5496 2635
Size: 1698 865 298 3347
Fragments arranged by size:
3347 1698 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4871 882
Size: 617 3372 2219
Fragments arranged by size:
3372 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3143 3505 3999 493
Size: 711 1939 362 494 2702
Fragments arranged by size:
2702 1939 711 494 362
BsaBI GATnn'nnATC
Cuts at: 6202 6202
Size: 6208
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3047 3435 4015 4397
Size: 53 83 186 936 1514 388 580 382
Cuts at: 4397 5032 275
Size: 635 1451
Fragments arranged by size:
1514 1451 936 635 580 388 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4641 5039 5313 5460 5871 4641
Size: 398 274 147 411 4978
Fragments arranged by size:
4978 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2363 2393 2861 2891 3305
Size: 30 165 30 960 30 468 30 414
Cuts at: 3305 3335 1178
Size: 30 4051
Fragments arranged by size:
4051 960 468 414 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2087 2291 2639 2669 2723 3031 3341 1186
Size: 901 204 348 30 54 308 310 4053
Fragments arranged by size:
4053 901 348 310 308 204 54 30
BseYI C'CCAG_C
Cuts at: 2166 3058 3265 5411 5907 2166
Size: 892 207 2146 496 2467
Fragments arranged by size:
2467 2146 892 496 207
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2195 2315 2444 2485 2800 3035 1163
Size: 573 459 120 129 41 315 235 4336
Fragments arranged by size:
4336 573 459 315 235 129 120 41
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3658 4419 5447 1540
Size: 275 186 1657 761 1028 2301
Fragments arranged by size:
2301 1657 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3430 4269 4354 5425 3430
Size: 839 85 1071 4213
Fragments arranged by size:
4213 1071 839 85
BsmI GAATG_Cn'
Cuts at: 3719 3812 3719
Size: 93 6115
BsmBI CGTCTCn'nnnn_
Cuts at: 2705 2705
Size: 6208
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2418 2879 3065 3086 3430 3527 4269
Size: 399 628 461 186 21 344 97 742
Cuts at: 4269 4354 5425 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 628 461 399 344 186
97 85 21
BspEI T'CCGG_A
Cuts at: 5871 5871
Size: 6208
BspHI T'CATG_A
Cuts at: 2781 4097 5827 2781
Size: 1316 1730 3162
Fragments arranged by size:
3162 1730 1316
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2167 2198 2235 3007 3563 844
Size: 1323 31 37 772 556 3489
Fragments arranged by size:
3489 1323 772 556 37 31
BsrBI CCG'CTC
Cuts at: 1394 1823 2029 2061 2740 4095 1394
Size: 429 206 32 679 1355 3507
Fragments arranged by size:
3507 1355 679 429 206 32
BsrDI GCAATG_nn'
Cuts at: 65 1248 4703 4877 65
Size: 1183 3455 174 1396
Fragments arranged by size:
3455 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2428 4852 5039 1700
Size: 110 618 2424 187 2869
Fragments arranged by size:
2869 2424 618 187 110
BsrGI T'GTAC_A
Cuts at: 96 3372 96
Size: 3276 2932
BssHII G'CGCG_C
Cuts at: 1925 2289 2291 1925
Size: 364 2 5842
Fragments arranged by size:
5842 364 2
BssSI C'ACGA_G
Cuts at: 4268 5280 4268
Size: 1012 5196
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2422 2485 794
Size: 1628 63 4517
Fragments arranged by size:
4517 1628 63
BstBI TT'CG_AA
Cuts at: 2007 5012 2007
Size: 3005 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6208
BstYI r'GATC_y
Cuts at: 1972 2580 3538 3905 4290 4307 5759 5845 5857
Size: 608 958 367 385 17 1452 86 12
Cuts at: 5857 6203 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 958 608 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2146 2335 2646 2820 3001 513
Size: 552 957 124 189 311 174 181 3720
Fragments arranged by size:
3720 957 552 311 189 181 174 124
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2521 487
Size: 683 56 1295 4174
Fragments arranged by size:
4174 1295 683 56
BtsI GCAGTG_nn'
Cuts at: 2439 2483 3247 3361 3724 4518 4538 2439
Size: 44 764 114 363 794 20 4109
Fragments arranged by size:
4109 794 764 363 114 44 20
ClaI AT'CG_AT
Cuts at: 1554 2856 1554
Size: 1302 4906
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6173
DraI TTT'AAA
Cuts at: 3512 3512
Size: 6208
DraIII CAC_nnn'GTG
Cuts at: 2422 3253 2422
Size: 831 5377
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5215 809
Size: 507 3899 1802
Fragments arranged by size:
3899 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2131 2649 2679
Size: 54 1425 50 275 18 301 518 30
Cuts at: 2679 3288 3655 4544 6173 8
Size: 609 367 889 1629 43
Fragments arranged by size:
1629 1425 889 609 518 367 301 275
54 50 43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 3655 1537
Size: 275 1843 4090
Fragments arranged by size:
4090 1843 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2357 2816 4138 1353
Size: 1004 459 1322 3423
Fragments arranged by size:
3423 1322 1004 459
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4795 5169 5315 387
Size: 1064 138 3206 374 146 1280
Fragments arranged by size:
3206 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2917 3145 3338 3429 4271 4868 5655
Size: 393 1285 228 193 91 842 597 787
Cuts at: 5655 5923 1239
Size: 268 1524
Fragments arranged by size:
1524 1285 842 787 597 393 268 228
193 91
EcoRI G'AATT_C
Cuts at: 3517 3895 3517
Size: 378 5830
FspI TGC'GCA
Cuts at: 2231 3458 4714 2231
Size: 1227 1256 3725
Fragments arranged by size:
3725 1256 1227
HaeII r_GCGC'y
Cuts at: 1678 2524 3050 3165 3361 3438 5355 1678
Size: 846 526 115 196 77 1917 2531
Fragments arranged by size:
2531 1917 846 526 196 115 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2198 2230 3185 3217 4849
Size: 32 135 32 626 32 955 32 1632
Cuts at: 4849 4881 4923 4955 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1632 955 626 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3552 3798 669
Size: 988 281 1614 246 3079
Fragments arranged by size:
3079 1614 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2600 748
Size: 1852 4356
HpaI GTT'AAC
Cuts at: 3798 3798
Size: 6208
KasI G'GCGC_C
Cuts at: 3046 3434 3046
Size: 388 5820
KpnI G_GTAC'C
Cuts at: 3533 3533
Size: 6208
MfeI C'AATT_G
Cuts at: 3807 3807
Size: 6208
MluI A'CGCG_T
Cuts at: 6084 6084
Size: 6208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5322 5506 1305
Size: 4017 184 2007
Fragments arranged by size:
4017 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3290 10
Size: 54 1425 343 1458 2928
Fragments arranged by size:
2928 1458 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3477 4166 4525 4684 518
Size: 648 2311 689 359 159 2042
Fragments arranged by size:
2311 2042 689 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2148 2163 2337 2957 3183 3624 4301 5449
Size: 542 15 174 620 226 441 677 1148
Cuts at: 5449 5694 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 620 542 441 245 226
174 15
NarI GG'CG_CC
Cuts at: 3047 3435 3047
Size: 388 5820
NcoI C'CATG_G
Cuts at: 513 1065 2022 2646 2820 3001 513
Size: 552 957 624 174 181 3720
Fragments arranged by size:
3720 957 624 552 181 174
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6208
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6208
NotI GC'GGCC_GC
Cuts at: 3655 3655
Size: 6208
NruI TCG'CGA
Cuts at: 2607 2607
Size: 6208
NspI r_CATG'y
Cuts at: 1132 1229 2483 2851 3566 5111 1132
Size: 97 1254 368 715 1545 2229
Fragments arranged by size:
2229 1545 1254 715 368 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6208
PciI A'CATG_T
Cuts at: 5107 5107
Size: 6208
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6208
PmeI GTTT'AAAC
Cuts at: 3512 3512
Size: 6208
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4277 4309 5790 5822 4277
Size: 32 1481 32 4663
Fragments arranged by size:
4663 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6208
PsiI TTA'TAA
Cuts at: 3778 3778
Size: 6208
PspXI vC'TCGA_Gb
Cuts at: 2360 2360
Size: 6208
PstI C_TGCA'G
Cuts at: 830 2748 2886 3120 3560 830
Size: 1918 138 234 440 3478
Fragments arranged by size:
3478 1918 440 234 138
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6208
PvuII CAG'CTG
Cuts at: 2957 3183 2957
Size: 226 5982
SacII CC_GC'GG
Cuts at: 2149 2338 2149
Size: 189 6019
SalI G'TCGA_C
Cuts at: 1936 3550 1936
Size: 1614 4594
SbfI CC_TGCA'GG
Cuts at: 3560 3560
Size: 6208
ScaI AGT'ACT
Cuts at: 4456 6168 4456
Size: 1712 4496
SexAI A'CCwGG_T
Cuts at: 2253 2253
Size: 6208
SfcI C'TryA_G
Cuts at: 826 1045 2744 2882 3116 3556 4691 5372 5563
Size: 219 1699 138 234 440 1135 681 191
Cuts at: 5563 826
Size: 1471
Fragments arranged by size:
1699 1471 1135 681 440 234 219 191
138
SfiI GGCCn_nnn'nGGCC
Cuts at: 2510 2510
Size: 6208
SfoI GGC'GCC
Cuts at: 3048 3436 3048
Size: 388 5820
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6208
SmaI CCC'GGG
Cuts at: 1872 2050 3535 1872
Size: 178 1485 4545
Fragments arranged by size:
4545 1485 178
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2360 2614 4312 5213 5475 820
Size: 197 926 417 254 1698 901 262 1553
Fragments arranged by size:
1698 1553 926 901 417 262 254 197
SnaBI TAC'GTA
Cuts at: 493 3505 3999 493
Size: 3012 494 2702
Fragments arranged by size:
3012 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6208
SphI G_CATG'C
Cuts at: 1132 2483 2851 3566 1132
Size: 1351 368 715 3774
Fragments arranged by size:
3774 1351 715 368
SspI AAT'ATT
Cuts at: 5 52 4132 5
Size: 47 4080 2081
Fragments arranged by size:
4080 2081 47
StuI AGG'CCT
Cuts at: 1517 2504 1517
Size: 987 5221
StyI C'CwwG_G
Cuts at: 513 1065 2022 2499 2646 2820 3001 3106 3647
Size: 552 957 477 147 174 181 105 541
Cuts at: 3647 513
Size: 3074
Fragments arranged by size:
3074 957 552 541 477 181 174 147
105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4235 4252 4405 4590 5035 325
Size: 1255 2655 17 153 185 445 1498
Fragments arranged by size:
2655 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3372 4454 6166
Size: 274 80 33 51 1443 1395 1082 1712
Cuts at: 6166 96
Size: 138
Fragments arranged by size:
1712 1443 1395 1082 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2363 2395 3305 3337 6168 6200 2363
Size: 32 910 32 2831 32 2371
Fragments arranged by size:
2831 2371 910 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2114 1316
Size: 798 5410
XbaI T'CTAG_A
Cuts at: 3544 3544
Size: 6208
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3277 3389 3277
Size: 112 6096
XhoI C'TCGA_G
Cuts at: 1943 2360 1943
Size: 417 5791
XmaI C'CCGG_G
Cuts at: 1870 2048 3533 1870
Size: 178 1485 4545
Fragments arranged by size:
4545 1485 178
XmnI GAAnn'nnTTC
Cuts at: 4337 4337
Size: 6208
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4016 276
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NruI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspXI PstI PvuI PvuII SacII
SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI
BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI
FseI FspAI NaeI NgoMIV NsiI PacI PfoI PmlI
PpuMI PspOMI PsrI RsrII SacI SanDI SapI SrfI
SwaI