Restriction map for
pFN21ASDA1092
(Circular) (Six-base) MAPSORT of: pFN21ASDA1092.seq Check: 8998 from: 1 to: 8110
pFN21ASDA1092 8110 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5920 278
Size: 53 83 186 936 4384 2468
Fragments arranged by size:
4384 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5453 1937
Size: 3516 4594
Acc65I G'GTAC_C
Cuts at: 2763 2990 5431 2763
Size: 227 2441 5442
Fragments arranged by size:
5442 2441 227
AclI AA'CG_TT
Cuts at: 1563 4030 6237 6610 1563
Size: 2467 2207 373 3063
Fragments arranged by size:
3063 2467 2207 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2682 3537 4225 5033 6173 7557 7825 1632
Size: 1050 855 688 808 1140 1384 268 1917
Fragments arranged by size:
1917 1384 1140 1050 855 808 688 268
AflII C'TTAA_G
Cuts at: 820 1017 2951 820
Size: 197 1934 5979
Fragments arranged by size:
5979 1934 197
AflIII A'CryG_T
Cuts at: 1203 7009 7986 1203
Size: 5806 977 1327
Fragments arranged by size:
5806 1327 977
AgeI A'CCGG_T
Cuts at: 6941 6941
Size: 8110
AhdI GACnn_n'nnGTC
Cuts at: 6839 6839
Size: 8110
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8110
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8110
AlwNI CAG_nnn'CTG
Cuts at: 3894 5081 5853 7425 3894
Size: 1187 772 1572 4579
Fragments arranged by size:
4579 1572 1187 772
ApaI G_GGCC'C
Cuts at: 2062 2081 4932 2062
Size: 19 2851 5240
Fragments arranged by size:
5240 2851 19
ApaLI G'TGCA_C
Cuts at: 4427 6167 7323 4427
Size: 1740 1156 5214
Fragments arranged by size:
5214 1740 1156
ApoI r'AATT_y
Cuts at: 1493 2493 3011 3051 3089 3917 4469 4541 5123
Size: 1000 518 40 38 828 552 72 582
Cuts at: 5123 5419 5645 5797 7906 1493
Size: 296 226 152 2109 1697
Fragments arranged by size:
2109 1697 1000 828 582 552 518 296
226 152 72 40 38
AseI AT'TA_AT
Cuts at: 160 1032 2442 6664 160
Size: 872 1410 4222 1606
Fragments arranged by size:
4222 1606 1410 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8110
AvaI C'yCGr_G
Cuts at: 1870 1943 2074 2226 2301 5435 1870
Size: 73 131 152 75 3134 4545
Fragments arranged by size:
4545 3134 152 131 75 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2747 2780 2982 3015 2747
Size: 33 202 33 7842
Fragments arranged by size:
7842 202 33 33
BamHI G'GATC_C
Cuts at: 5440 5807 5440
Size: 367 7743
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2045 2090 2099 2763 2990
Size: 325 217 627 258 45 9 664 227
Cuts at: 2990 4340 5150 5324 5431 6886 618
Size: 1350 810 174 107 1455 1842
Fragments arranged by size:
1842 1455 1350 810 664 627 325 258
227 217 174 107 45 9
BanII G_rGCy'C
Cuts at: 1391 2062 2081 3409 4932 5429 1391
Size: 671 19 1328 1523 497 4072
Fragments arranged by size:
4072 1523 1328 671 497 19
BbeI G_GCGC'C
Cuts at: 2049 2094 2103 2049
Size: 45 9 8056
Fragments arranged by size:
8056 45 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 7139
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6301 6335 1903
Size: 34 4364 34 3678
Fragments arranged by size:
4364 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3570 6002 7212 3570
Size: 2432 1210 4468
Fragments arranged by size:
4468 2432 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8110
BfrBI ATG'CAT
Cuts at: 3542 4739 3542
Size: 1197 6913
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2043 2057 6721
Size: 107 122 71 631 605 371 14 4664
Cuts at: 6721 136
Size: 1525
Fragments arranged by size:
4664 1525 631 605 371 122 107 71
14
BglII A'GATC_T
Cuts at: 4816 5236 8105 4816
Size: 420 2869 4821
Fragments arranged by size:
4821 2869 420
BlpI GC'TnA_GC
Cuts at: 5527 5527
Size: 8110
Bme1580I G_kGCm'C
Cuts at: 1790 2062 2081 4431 4932 6171 7327 1790
Size: 272 19 2350 501 1239 1156 2573
Fragments arranged by size:
2573 2350 1239 1156 501 272 19
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8110
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3958 4047 5841
Size: 416 53 83 399 24 2541 89 1794
Cuts at: 5841 6799 442
Size: 958 1753
Fragments arranged by size:
2541 1794 1753 958 416 399 89 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2235 1056
Size: 1179 6931
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2162 3665 4249 2162
Size: 1503 584 6023
Fragments arranged by size:
6023 1503 584
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4638 5418 6770 1239
Size: 3399 780 1352 2579
Fragments arranged by size:
3399 2579 1352 780
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3143 4074 5039 6235 7100 7398 3143
Size: 931 965 1196 865 298 3855
Fragments arranged by size:
3855 1196 965 931 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4237 4279 6773 882
Size: 617 2738 42 2494 2219
Fragments arranged by size:
2738 2494 2219 617 42
BsaAI yAC'GTr
Cuts at: 493 1204 4654 5407 5901 493
Size: 711 3450 753 494 2702
Fragments arranged by size:
3450 2702 753 711 494
BsaBI GATnn'nnATC
Cuts at: 8104 8104
Size: 8110
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2046 2091 2100 5917
Size: 53 83 186 936 513 45 9 3817
Cuts at: 5917 6299 6934 275
Size: 382 635 1451
Fragments arranged by size:
3817 1451 936 635 513 382 186 83
53 45 9
BsaWI w'CCGG_w
Cuts at: 6543 6941 7215 7362 7773 6543
Size: 398 274 147 411 6880
Fragments arranged by size:
6880 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5391 5421 1178
Size: 30 165 30 3988 30 3867
Fragments arranged by size:
3988 3867 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4241 4689 4692 4864 1186
Size: 3055 448 3 172 4432
Fragments arranged by size:
4432 3055 448 172 3
BseYI C'CCAG_C
Cuts at: 2276 4791 7313 7809 2276
Size: 2515 2522 496 2577
Fragments arranged by size:
2577 2522 2515 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4425 1163
Size: 573 2689 4848
Fragments arranged by size:
4848 2689 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2038 2286 5560 6321 7349 1540
Size: 275 186 37 248 3274 761 1028 2301
Fragments arranged by size:
3274 2301 1028 761 275 248 186 37
BsiHKAI G_wGCw'C
Cuts at: 3409 4431 6171 6256 7327 3409
Size: 1022 1740 85 1071 4192
Fragments arranged by size:
4192 1740 1071 1022 85
BsmI GAATG_Cn'
Cuts at: 3542 4792 4843 5621 5714 3542
Size: 1250 51 778 93 5938
Fragments arranged by size:
5938 1250 778 93 51
BsmBI CGTCTCn'nnnn_
Cuts at: 4434 4434
Size: 8110
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2062 2081 3409 4431 4932 5429 6171
Size: 399 272 19 1328 1022 501 497 742
Cuts at: 6171 6256 7327 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1328 1071 1022 742 501 497 399
272 85 19
BspEI T'CCGG_A
Cuts at: 7773 7773
Size: 8110
BspHI T'CATG_A
Cuts at: 3028 4218 5999 7729 3028
Size: 1190 1781 1730 3409
Fragments arranged by size:
3409 1781 1730 1190
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4491 5465 844
Size: 3647 974 3489
Fragments arranged by size:
3647 3489 974
BsrBI CCG'CTC
Cuts at: 1394 1823 5997 1394
Size: 429 4174 3507
Fragments arranged by size:
4174 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2644 2909 6605 6779 65
Size: 1183 1396 265 3696 174 1396
Fragments arranged by size:
3696 1396 1396 1183 265 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2051 6754 6941 1700
Size: 110 241 4703 187 2869
Fragments arranged by size:
4703 2869 241 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8110
BssHII G'CGCG_C
Cuts at: 1925 2162 1925
Size: 237 7873
BssSI C'ACGA_G
Cuts at: 6170 7182 6170
Size: 1012 7098
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2735 3548 794
Size: 1941 813 5356
Fragments arranged by size:
5356 1941 813
BstBI TT'CG_AA
Cuts at: 2007 6914 2007
Size: 4907 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8110
BstYI r'GATC_y
Cuts at: 1972 4360 4816 5236 5440 5807 6192 6209 7661
Size: 2388 456 420 204 367 385 17 1452
Cuts at: 7661 7747 7759 8105 1972
Size: 86 12 346 1977
Fragments arranged by size:
2388 1977 1452 456 420 385 367 346
204 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2537 2537
Size: 8110
BtgI C'CryG_G
Cuts at: 513 1065 2022 2152 2251 4384 4497 4767 5118
Size: 552 957 130 99 2133 113 270 351
Cuts at: 5118 513
Size: 3505
Fragments arranged by size:
3505 2133 957 552 351 270 130 113
99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7371
Fragments arranged by size:
7371 683 56
BtsI GCAGTG_nn'
Cuts at: 2431 3520 4901 5196 5626 6420 6440 2431
Size: 1089 1381 295 430 794 20 4101
Fragments arranged by size:
4101 1381 1089 794 430 295 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8110
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8075
DraI TTT'AAA
Cuts at: 2931 5414 2931
Size: 2483 5627
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7117 809
Size: 507 5801 1802
Fragments arranged by size:
5801 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2035 2149 4208
Size: 54 1425 50 275 18 205 114 2059
Cuts at: 4208 4494 4549 4881 5557 6446 8075 8
Size: 286 55 332 676 889 1629 43
Fragments arranged by size:
2059 1629 1425 889 676 332 286 275
205 114 55 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2035 5557 1537
Size: 275 223 3522 4090
Fragments arranged by size:
4090 3522 275 223
EarI CTCTTCn'nnn_
Cuts at: 1353 2360 4666 6040 1353
Size: 1007 2306 1374 3423
Fragments arranged by size:
3423 2306 1374 1007
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2042 6697 7071 7217 387
Size: 1064 138 453 4655 374 146 1280
Fragments arranged by size:
4655 1280 1064 453 374 146 138
EcoICRI GAG'CTC
Cuts at: 3407 3407
Size: 8110
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2682 3537 4225 4638 5033 5418 6173
Size: 393 1050 855 688 413 395 385 755
Cuts at: 6173 6770 7557 7825 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1050 855 787 755 688 597 413
395 393 385 268
EcoO109I rG'GnC_Cy
Cuts at: 2058 2059 2246 3106 3377 4624 4699 4928 2058
Size: 1 187 860 271 1247 75 229 5240
Fragments arranged by size:
5240 1247 860 271 229 187 75 1
EcoRI G'AATT_C
Cuts at: 3089 4541 5123 5419 5797 3089
Size: 1452 582 296 378 5402
Fragments arranged by size:
5402 1452 582 378 296
EcoRV GAT'ATC
Cuts at: 2658 2658
Size: 8110
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3110 5255 3110
Size: 2145 5965
FspI TGC'GCA
Cuts at: 6616 6616
Size: 8110
HaeII r_GCGC'y
Cuts at: 1678 2049 2094 2103 4248 7257 1678
Size: 371 45 9 2145 3009 2531
Fragments arranged by size:
3009 2531 2145 371 45 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2573 2605 2642 2674 6751
Size: 32 135 32 1001 32 37 32 4077
Cuts at: 6751 6783 6825 6857 1373
Size: 32 42 32 2626
Fragments arranged by size:
4077 2626 1001 135 42 37 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5454 5700 669
Size: 988 281 3516 246 3079
Fragments arranged by size:
3516 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4462 748
Size: 3714 4396
HpaI GTT'AAC
Cuts at: 5700 5700
Size: 8110
KasI G'GCGC_C
Cuts at: 2045 2090 2099 2045
Size: 45 9 8056
Fragments arranged by size:
8056 45 9
KpnI G_GTAC'C
Cuts at: 2767 2994 5435 2767
Size: 227 2441 5442
Fragments arranged by size:
5442 2441 227
MfeI C'AATT_G
Cuts at: 5709 5709
Size: 8110
MluI A'CGCG_T
Cuts at: 7986 7986
Size: 8110
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2211 2508 3408 7224 7408 1305
Size: 906 297 900 3816 184 2007
Fragments arranged by size:
3816 2007 906 900 297 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4496 4883 10
Size: 54 1425 343 2664 387 3237
Fragments arranged by size:
3237 2664 1425 387 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3150 3183 4434 6068 6427 6586 518
Size: 648 1984 33 1251 1634 359 159 2042
Fragments arranged by size:
2042 1984 1634 1251 648 359 159 33
MspA1I CmG'CkG
Cuts at: 1606 2175 2253 5526 6203 7351 7596 1606
Size: 569 78 3273 677 1148 245 2120
Fragments arranged by size:
3273 2120 1148 677 569 245 78
NaeI GCC'GGC
Cuts at: 2053 2053
Size: 8110
NarI GG'CG_CC
Cuts at: 2046 2091 2100 2046
Size: 45 9 8056
Fragments arranged by size:
8056 45 9
NcoI C'CATG_G
Cuts at: 513 1065 2022 4384 4497 5118 513
Size: 552 957 2362 113 621 3505
Fragments arranged by size:
3505 2362 957 621 552 113
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8110
NgoMIV G'CCGG_C
Cuts at: 2051 2051
Size: 8110
NheI G'CTAG_C
Cuts at: 1052 2231 1052
Size: 1179 6931
NotI GC'GGCC_GC
Cuts at: 5557 5557
Size: 8110
NsiI A_TGCA'T
Cuts at: 3544 4741 3544
Size: 1197 6913
NspI r_CATG'y
Cuts at: 1132 1229 2404 2530 4637 4834 4903 5468 7013
Size: 97 1175 126 2107 197 69 565 1545
Cuts at: 7013 1132
Size: 2229
Fragments arranged by size:
2229 2107 1545 1175 565 197 126 97
69
PasI CC'CwG_GG
Cuts at: 1348 4268 1348
Size: 2920 5190
PciI A'CATG_T
Cuts at: 7009 7009
Size: 8110
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8110
PmeI GTTT'AAAC
Cuts at: 5414 5414
Size: 8110
PmlI CAC'GTG
Cuts at: 4654 4654
Size: 8110
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2939 2971 3353 3385 6179 6211 7692 7724 2939
Size: 32 382 32 2794 32 1481 32 3325
Fragments arranged by size:
3325 2794 1481 382 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2246 3106 3377 4624 2246
Size: 860 271 1247 5732
Fragments arranged by size:
5732 1247 860 271
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8110
PsiI TTA'TAA
Cuts at: 3332 5680 3332
Size: 2348 5762
PspOMI G'GGCC_C
Cuts at: 2058 2077 4928 2058
Size: 19 2851 5240
Fragments arranged by size:
5240 2851 19
PstI C_TGCA'G
Cuts at: 830 2201 4608 4992 5462 830
Size: 1371 2407 384 470 3478
Fragments arranged by size:
3478 2407 1371 470 384
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8110
SacI G_AGCT'C
Cuts at: 3409 3409
Size: 8110
SacII CC_GC'GG
Cuts at: 2254 2254
Size: 8110
SalI G'TCGA_C
Cuts at: 1936 5452 1936
Size: 3516 4594
SanDI GG'GwC_CC
Cuts at: 2246 2246
Size: 8110
SbfI CC_TGCA'GG
Cuts at: 5462 5462
Size: 8110
ScaI AGT'ACT
Cuts at: 3947 6358 8070 3947
Size: 2411 1712 3987
Fragments arranged by size:
3987 2411 1712
SfcI C'TryA_G
Cuts at: 826 1045 2197 4604 4988 5458 6593 7274 7465
Size: 219 1152 2407 384 470 1135 681 191
Cuts at: 7465 826
Size: 1471
Fragments arranged by size:
2407 1471 1152 1135 681 470 384 219
191
SfoI GGC'GCC
Cuts at: 2047 2092 2101 2047
Size: 45 9 8056
Fragments arranged by size:
8056 45 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8110
SgrAI Cr'CCGG_yG
Cuts at: 1700 2051 1700
Size: 351 7759
SmaI CCC'GGG
Cuts at: 1872 2076 2228 2303 5437 1872
Size: 204 152 75 3134 4545
Fragments arranged by size:
4545 3134 204 152 75
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2951 3122 4089 5054 6214 7115
Size: 197 926 1008 171 967 965 1160 901
Cuts at: 7115 7377 820
Size: 262 1553
Fragments arranged by size:
1553 1160 1008 967 965 926 901 262
197 171
SnaBI TAC'GTA
Cuts at: 493 5407 5901 493
Size: 4914 494 2702
Fragments arranged by size:
4914 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8110
SphI G_CATG'C
Cuts at: 1132 2404 5468 1132
Size: 1272 3064 3774
Fragments arranged by size:
3774 3064 1272
SrfI GCCC'GGGC
Cuts at: 2228 2228
Size: 8110
SspI AAT'ATT
Cuts at: 5 52 4165 5212 6034 5
Size: 47 4113 1047 822 2081
Fragments arranged by size:
4113 2081 1047 822 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8110
StyI C'CwwG_G
Cuts at: 513 1065 2022 2094 2872 3102 4384 4497 5118
Size: 552 957 72 778 230 1282 113 621
Cuts at: 5118 5154 5549 513
Size: 36 395 3074
Fragments arranged by size:
3074 1282 957 778 621 552 395 230
113 72 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3679 6137 6154 6307 6492 6937 325
Size: 1255 2099 2458 17 153 185 445 1498
Fragments arranged by size:
2458 2099 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3038 3945 4554
Size: 274 80 33 51 1443 1061 907 609
Cuts at: 4554 6356 8068 96
Size: 1802 1712 138
Fragments arranged by size:
1802 1712 1443 1061 907 609 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8070 8102 8070
Size: 32 8078
Tth111I GACn'n_nGTC
Cuts at: 1316 4262 1316
Size: 2946 5164
XbaI T'CTAG_A
Cuts at: 2489 5446 2489
Size: 2957 5153
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4351 4351
Size: 8110
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8110
XmaI C'CCGG_G
Cuts at: 1870 2074 2226 2301 5435 1870
Size: 204 152 75 3134 4545
Fragments arranged by size:
4545 3134 204 152 75
XmnI GAAnn'nnTTC
Cuts at: 3174 4069 6239 3174
Size: 895 2170 5045
Fragments arranged by size:
5045 2170 895
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5918 276
Size: 53 83 186 936 4384 2468
Fragments arranged by size:
4384 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII
SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbvCI Bpu10I BsiWI
BstXI BstZ17I DraIII EcoNI FseI FspAI NruI PacI
PfoI PspXI PsrI PvuII RsrII SapI SexAI SfiI
SwaI