Restriction map for
pFN21ASDA1088
(Circular) (Six-base) MAPSORT of: pFN21ASDA1088.seq Check: 4592 from: 1 to: 8929
pFN21ASDA1088 8929 bp
With 182 enzymes: SgfI *
February 1, 2012 16:19 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2856 6739 278
Size: 53 83 186 936 1320 3883 2468
Fragments arranged by size:
3883 2468 1320 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2055 6272 1937
Size: 118 4217 4594
Fragments arranged by size:
4594 4217 118
Acc65I G'GTAC_C
Cuts at: 4068 6250 4068
Size: 2182 6747
AclI AA'CG_TT
Cuts at: 1563 7056 7429 1563
Size: 5493 373 3063
Fragments arranged by size:
5493 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2687 2768 2826 2996 3102 3379 3797 4439
Size: 1055 81 58 170 106 277 418 642
Cuts at: 4439 5825 6992 8376 8644 1632
Size: 1386 1167 1384 268 1917
Fragments arranged by size:
1917 1386 1384 1167 1055 642 418 277
268 170 106 81 58
AfeI AGC'GCT
Cuts at: 5268 5402 5813 6189 5268
Size: 134 411 376 8008
Fragments arranged by size:
8008 411 376 134
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8732
AflIII A'CryG_T
Cuts at: 1203 6135 7828 8805 1203
Size: 4932 1693 977 1327
Fragments arranged by size:
4932 1693 1327 977
AgeI A'CCGG_T
Cuts at: 7760 7760
Size: 8929
AhdI GACnn_n'nnGTC
Cuts at: 2852 7658 2852
Size: 4806 4123
AleI CACnn'nnGTG
Cuts at: 1166 5413 6099 1166
Size: 4247 686 3996
Fragments arranged by size:
4247 3996 686
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5396 6117 1184
Size: 4212 721 3996
Fragments arranged by size:
4212 3996 721
AlwNI CAG_nnn'CTG
Cuts at: 2581 2869 3148 3196 4009 4499 4777 5068 5597
Size: 288 279 48 813 490 278 291 529
Cuts at: 5597 5666 6672 8244 2581
Size: 69 1006 1572 3266
Fragments arranged by size:
3266 1572 1006 813 529 490 291 288
279 278 69 48
ApaI G_GGCC'C
Cuts at: 3748 5233 5733 3748
Size: 1485 500 6944
Fragments arranged by size:
6944 1485 500
ApaLI G'TGCA_C
Cuts at: 2182 3290 4692 5416 6986 8142 2182
Size: 1108 1402 724 1570 1156 2969
Fragments arranged by size:
2969 1570 1402 1156 1108 724
ApoI r'AATT_y
Cuts at: 1493 6238 6464 6616 8725 1493
Size: 4745 226 152 2109 1697
Fragments arranged by size:
4745 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7483 160
Size: 872 6451 1606
Fragments arranged by size:
6451 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8929
AvaI C'yCGr_G
Cuts at: 1870 1943 2442 3984 4305 4457 5443 6254 1870
Size: 73 499 1542 321 152 986 811 4545
Fragments arranged by size:
4545 1542 986 811 499 321 152 73
BamHI G'GATC_C
Cuts at: 5029 6259 6626 5029
Size: 1230 367 7332
Fragments arranged by size:
7332 1230 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3676 3869 4068 4122 5023
Size: 325 217 627 1889 193 199 54 901
Cuts at: 5023 5779 5835 5999 6093 6250 7705 618
Size: 756 56 164 94 157 1455 1842
Fragments arranged by size:
1889 1842 1455 901 756 627 325 217
199 193 164 157 94 56 54
BanII G_rGCy'C
Cuts at: 1391 2150 2441 2907 3592 3602 3690 3748 3798
Size: 759 291 466 685 10 88 58 50
Cuts at: 3798 4189 4565 4683 5233 5512 5722 5733 5919
Size: 391 376 118 550 279 210 11 186
Cuts at: 5919 6248 1391
Size: 329 4072
Fragments arranged by size:
4072 759 685 550 466 391 376 329
291 279 210 186 118 88 58 50
11 10
BbeI G_GCGC'C
Cuts at: 3873 5027 3873
Size: 1154 7775
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2171 2967 4138 5546 5555 5896 928
Size: 971 272 796 1171 1408 9 341 3961
Fragments arranged by size:
3961 1408 1171 971 796 341 272 9
BbvCI CC'TCA_GC
Cuts at: 4842 4842
Size: 8929
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7120 7154 1903
Size: 34 5183 34 3678
Fragments arranged by size:
5183 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4937 6821 8031 4937
Size: 1884 1210 5835
Fragments arranged by size:
5835 1884 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8929
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2246 3685 4307
Size: 107 122 71 631 605 574 1439 622
Cuts at: 4307 4519 4814 5213 5228 6091 7540 136
Size: 212 295 399 15 863 1449 1525
Fragments arranged by size:
1525 1449 1439 863 631 622 605 574
399 295 212 122 107 71 15
BglII A'GATC_T
Cuts at: 3552 5562 8924 3552
Size: 2010 3362 3557
Fragments arranged by size:
3557 3362 2010
BlpI GC'TnA_GC
Cuts at: 3327 3380 6346 3327
Size: 53 2966 5910
Fragments arranged by size:
5910 2966 53
Bme1580I G_kGCm'C
Cuts at: 1790 2186 2645 3294 3681 3748 4696 4993 5172
Size: 396 459 649 387 67 948 297 179
Cuts at: 5172 5233 5420 5733 5784 5795 5861 6087 6990
Size: 61 187 313 51 11 66 226 903
Cuts at: 6990 8146 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 948 903 649 459 396 387
313 297 226 187 179 67 66 61
51 11
BmgBI CAC'GTC
Cuts at: 1318 3158 4216 4255 5415 5776 6136 1318
Size: 1840 1058 39 1160 361 360 4111
Fragments arranged by size:
4111 1840 1160 1058 361 360 39
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3506 3586 4255
Size: 416 53 83 399 24 2089 80 669
Cuts at: 4255 4314 6074 6660 7618 442
Size: 59 1760 586 958 1753
Fragments arranged by size:
2089 1760 1753 958 669 586 416 399
83 80 59 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8929
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2225 3342 2225
Size: 1117 7812
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2162 2331 2344 2591 2891 3017 3048 3183
Size: 923 169 13 247 300 126 31 135
Cuts at: 3183 3364 3512 3542 3752 4556 5184 5863 5961
Size: 181 148 30 210 804 628 679 98
Cuts at: 5961 6204 7589 1239
Size: 243 1385 2579
Fragments arranged by size:
2579 1385 923 804 679 628 300 247
243 210 181 169 148 135 126 98
31 30 13
Bpu10I CC'TnA_GC
Cuts at: 4842 4962 4842
Size: 120 8809
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3980 7054 7919 8217 3980
Size: 3074 865 298 4692
Fragments arranged by size:
4692 3074 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2307 3815 5127 7592 882
Size: 617 808 1508 1312 2465 2219
Fragments arranged by size:
2465 2219 1508 1312 808 617
BsaAI yAC'GTr
Cuts at: 493 1204 6226 6720 493
Size: 711 5022 494 2702
Fragments arranged by size:
5022 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3500 8923 3500
Size: 5423 3506
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2853 3435 3870 4082
Size: 53 83 186 936 1320 582 435 212
Cuts at: 4082 5024 6736 7118 7753 275
Size: 942 1712 382 635 1451
Fragments arranged by size:
1712 1451 1320 942 936 635 582 435
382 212 186 83 53
BsaWI w'CCGG_w
Cuts at: 3315 7362 7760 8034 8181 8592 3315
Size: 4047 398 274 147 411 3652
Fragments arranged by size:
4047 3652 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2438 2468 2780 2810 4574
Size: 30 165 30 1035 30 312 30 1764
Cuts at: 4574 4604 5704 5734 5861 5891 6210 6240 1178
Size: 30 1100 30 127 30 319 30 3867
Fragments arranged by size:
3867 1764 1100 1035 319 312 165 127
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2888 3641 4586 4653 4691 4931 5486 1186
Size: 1702 753 945 67 38 240 555 4629
Fragments arranged by size:
4629 1702 945 753 555 240 67 38
BseYI C'CCAG_C
Cuts at: 2434 2933 3256 3265 4037 4412 5061 5627 5699
Size: 499 323 9 772 375 649 566 72
Cuts at: 5699 5860 8132 8628 2434
Size: 161 2272 496 2735
Fragments arranged by size:
2735 2272 772 649 566 499 496 375
323 161 72 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5345 6123 1163
Size: 573 3609 778 3969
Fragments arranged by size:
3969 3609 778 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3183 5434 6111 6379 7140 8168
Size: 275 186 1182 2251 677 268 761 1028
Cuts at: 8168 1540
Size: 2301
Fragments arranged by size:
2301 2251 1182 1028 761 677 275 268
186
BsiHKAI G_wGCw'C
Cuts at: 2186 2734 3294 3350 3602 4542 4696 4968 5085
Size: 548 560 56 252 940 154 272 117
Cuts at: 5085 5420 5722 6990 7075 8146 2186
Size: 335 302 1268 85 1071 2969
Fragments arranged by size:
2969 1268 1071 940 560 548 335 302
272 252 154 117 85 56
BsiWI C'GTAC_G
Cuts at: 6222 6222
Size: 8929
BsmI GAATG_Cn'
Cuts at: 4971 6440 6533 4971
Size: 1469 93 7367
Fragments arranged by size:
7367 1469 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2208 3024 4245 2208
Size: 816 1221 6892
Fragments arranged by size:
6892 1221 816
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2150 2186 2441 2645 2734 2907 3294
Size: 399 360 36 255 204 89 173 387
Cuts at: 3294 3350 3592 3602 3681 3690 3748 3798 4189
Size: 56 242 10 79 9 58 50 391
Cuts at: 4189 4542 4565 4683 4696 4968 4993 5085 5172
Size: 353 23 118 13 272 25 92 87
Cuts at: 5172 5233 5420 5512 5722 5733 5784 5795 5861
Size: 61 187 92 210 11 51 11 66
Cuts at: 5861 5919 6087 6248 6990 7075 8146 1391
Size: 58 168 161 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 391 387 360 353
272 255 242 210 204 187 173 168
161 118 92 92 89 87 85 79
66 61 58 58 56 51 50 36
25 23 13 11 11 10 9
BspEI T'CCGG_A
Cuts at: 8592 8592
Size: 8929
BspHI T'CATG_A
Cuts at: 6818 8548 6818
Size: 1730 7199
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4410 4872 5612 5890 6284 844
Size: 3566 462 740 278 394 3489
Fragments arranged by size:
3566 3489 740 462 394 278
BsrBI CCG'CTC
Cuts at: 1394 1823 2622 3441 6065 6816 1394
Size: 429 799 819 2624 751 3507
Fragments arranged by size:
3507 2624 819 799 751 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2964 7424 7598 65
Size: 1183 1716 4460 174 1396
Fragments arranged by size:
4460 1716 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2415 3866 4071 4297 4621 4996 5429
Size: 110 605 1451 205 226 324 375 433
Cuts at: 5429 5433 5996 6002 7573 7760 1700
Size: 4 563 6 1571 187 2869
Fragments arranged by size:
2869 1571 1451 605 563 433 375 324
226 205 187 110 6 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8929
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8929
BssSI C'ACGA_G
Cuts at: 3416 6989 8001 3416
Size: 3573 1012 4344
Fragments arranged by size:
4344 3573 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3158 3848 4864 794
Size: 2364 690 1016 4859
Fragments arranged by size:
4859 2364 1016 690
BstBI TT'CG_AA
Cuts at: 2007 7733 2007
Size: 5726 3203
BstEII G'GTnAC_C
Cuts at: 1184 3311 4437 1184
Size: 2127 1126 5676
Fragments arranged by size:
5676 2127 1126
BstXI CCAn_nnnn'nTGG
Cuts at: 2107 3766 3880 5762 2107
Size: 1659 114 1882 5274
Fragments arranged by size:
5274 1882 1659 114
BstYI r'GATC_y
Cuts at: 1972 2499 3501 3552 4653 5029 5562 6259 6626
Size: 527 1002 51 1101 376 533 697 367
Cuts at: 6626 7011 7028 8480 8566 8578 8924 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1101 1002 697 533 527 385
376 367 346 86 51 17 12
Bsu36I CC'TnA_GG
Cuts at: 2421 2466 4432 5638 2421
Size: 45 1966 1206 5712
Fragments arranged by size:
5712 1966 1206 45
BtgI C'CryG_G
Cuts at: 513 1065 3937 4683 5049 5241 5650 5712 513
Size: 552 2872 746 366 192 409 62 3730
Fragments arranged by size:
3730 2872 746 552 409 366 192 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3649 5680 487
Size: 683 56 2423 2031 3736
Fragments arranged by size:
3736 2423 2031 683 56
BtsI GCAGTG_nn'
Cuts at: 3351 5331 6445 7239 7259 3351
Size: 1980 1114 794 20 5021
Fragments arranged by size:
5021 1980 1114 794 20
ClaI AT'CG_AT
Cuts at: 1554 3009 1554
Size: 1455 7474
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8894
DraI TTT'AAA
Cuts at: 6233 6233
Size: 8929
DraIII CAC_nnn'GTG
Cuts at: 2638 2785 4322 5200 6136 2638
Size: 147 1537 878 936 5431
Fragments arranged by size:
5431 1537 936 878 147
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3041 4406 7936 809
Size: 507 1725 1365 3530 1802
Fragments arranged by size:
3530 1802 1725 1365 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2583 3180 3403
Size: 54 1425 50 275 18 753 597 223
Cuts at: 3403 3756 3934 4237 4527 4725 4815 5319 5431
Size: 353 178 303 290 198 90 504 112
Cuts at: 5431 5909 6108 6376 7265 8894 8
Size: 478 199 268 889 1629 43
Fragments arranged by size:
1629 1425 889 753 597 504 478 353
303 290 275 268 223 199 198 178
112 90 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3180 5431 6108 6376 1537
Size: 275 1368 2251 677 268 4090
Fragments arranged by size:
4090 2251 1368 677 275 268
EarI CTCTTCn'nnn_
Cuts at: 1353 3719 4610 4721 6859 1353
Size: 2366 891 111 2138 3423
Fragments arranged by size:
3423 2366 2138 891 111
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3215 3416 7516 7890 8036 387
Size: 1064 138 1626 201 4100 374 146 1280
Fragments arranged by size:
4100 1626 1280 1064 374 201 146 138
EcoICRI GAG'CTC
Cuts at: 3600 5720 3600
Size: 2120 6809
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2162 2331 2344 2591 2687 2768 2826
Size: 393 530 169 13 247 96 81 58
Cuts at: 2826 2891 2996 3017 3048 3102 3183 3364 3379
Size: 65 105 21 31 54 81 181 15
Cuts at: 3379 3512 3542 3752 3797 4439 4556 5184 5825
Size: 133 30 210 45 642 117 628 641
Cuts at: 5825 5863 5961 6204 6992 7589 8376 8644 1239
Size: 38 98 243 788 597 787 268 1524
Fragments arranged by size:
1524 788 787 642 641 628 597 530
393 268 247 243 210 181 169 133
117 105 98 96 81 81 65 58
54 45 38 31 30 21 15 13
EcoNI CCTnn'n_nnAGG
Cuts at: 2573 3647 5090 5300 2573
Size: 1074 1443 210 6202
Fragments arranged by size:
6202 1443 1074 210
EcoO109I rG'GnC_Cy
Cuts at: 2104 2364 3270 3578 4428 4453 4774 4846 5100
Size: 260 906 308 850 25 321 72 254
Cuts at: 5100 5229 5230 5462 5522 5624 5642 5730 2104
Size: 129 1 232 60 102 18 88 5303
Fragments arranged by size:
5303 906 850 321 308 260 254 232
129 102 88 72 60 25 18 1
EcoRI G'AATT_C
Cuts at: 6238 6616 6238
Size: 378 8551
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2276 5419 2276
Size: 3143 5786
FseI GG_CCGG'CC
Cuts at: 5437 5437
Size: 8929
FspI TGC'GCA
Cuts at: 7435 7435
Size: 8929
HaeII r_GCGC'y
Cuts at: 1678 3776 3873 5027 5208 5270 5404 5815 5940
Size: 2098 97 1154 181 62 134 411 125
Cuts at: 5940 5982 6191 8076 1678
Size: 42 209 1885 2531
Fragments arranged by size:
2531 2098 1885 1154 411 209 181 134
125 97 62 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2205 2207 2237 2239 2476
Size: 32 135 32 633 2 30 2 237
Cuts at: 2476 2508 2838 2870 3620 3652 4340 4372 7570
Size: 32 330 32 750 32 688 32 3198
Cuts at: 7570 7602 7644 7676 1373
Size: 32 42 32 2626
Fragments arranged by size:
3198 2626 750 688 633 330 237 135
42 32 32 32 32 32 32 32
32 30 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 6273 6519 669
Size: 988 281 4335 246 3079
Fragments arranged by size:
4335 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8929
HpaI GTT'AAC
Cuts at: 6519 6519
Size: 8929
KasI G'GCGC_C
Cuts at: 3869 5023 3869
Size: 1154 7775
KpnI G_GTAC'C
Cuts at: 4072 6254 4072
Size: 2182 6747
MfeI C'AATT_G
Cuts at: 6528 6528
Size: 8929
MluI A'CGCG_T
Cuts at: 8805 8805
Size: 8929
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2691 8043 8227 1305
Size: 1386 5352 184 2007
Fragments arranged by size:
5352 2007 1386 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2585 3758 4239 5911 10
Size: 54 1425 343 753 1173 481 1672 3028
Fragments arranged by size:
3028 1672 1425 1173 753 481 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2690 3303 5378 5413 5668 6099 6887
Size: 648 1524 613 2075 35 255 431 788
Cuts at: 6887 7246 7405 518
Size: 359 159 2042
Fragments arranged by size:
2075 2042 1524 788 648 613 431 359
255 159 35
MspA1I CmG'CkG
Cuts at: 1606 2452 3039 3331 3459 3784 4445 4552 4587
Size: 846 587 292 128 325 661 107 35
Cuts at: 4587 4933 4987 5068 5243 6107 6345 7022 8170
Size: 346 54 81 175 864 238 677 1148
Cuts at: 8170 8415 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 864 846 677 661 587 346
325 292 245 238 175 128 107 81
54 35
NaeI GCC'GGC
Cuts at: 4623 5435 6004 4623
Size: 812 569 7548
Fragments arranged by size:
7548 812 569
NarI GG'CG_CC
Cuts at: 3870 5024 3870
Size: 1154 7775
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 8377
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8929
NgoMIV G'CCGG_C
Cuts at: 4621 5433 6002 4621
Size: 812 569 7548
Fragments arranged by size:
7548 812 569
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8929
NotI GC'GGCC_GC
Cuts at: 6376 6376
Size: 8929
NspI r_CATG'y
Cuts at: 1132 1229 3415 4409 5377 6287 7832 1132
Size: 97 2186 994 968 910 1545 2229
Fragments arranged by size:
2229 2186 1545 994 968 910 97
PasI CC'CwG_GG
Cuts at: 1348 2939 3643 3857 4515 5233 1348
Size: 1591 704 214 658 718 5044
Fragments arranged by size:
5044 1591 718 704 658 214
PciI A'CATG_T
Cuts at: 7828 7828
Size: 8929
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2836 2869 3148 5068 5260 1108
Size: 1728 33 279 1920 192 4777
Fragments arranged by size:
4777 1920 1728 279 192 33
PfoI T'CCnGG_A
Cuts at: 2106 2139 2265 2360 2595 2657 3117 4008 5001
Size: 33 126 95 235 62 460 891 993
Cuts at: 5001 5879 2106
Size: 878 5156
Fragments arranged by size:
5156 993 891 878 460 235 126 95
62 33
PmeI GTTT'AAAC
Cuts at: 6233 6233
Size: 8929
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6998 7030 8511 8543 6998
Size: 32 1481 32 7384
Fragments arranged by size:
7384 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2104 2364 4428 4453 4846 5462 5624 5642 2104
Size: 260 2064 25 393 616 162 18 5391
Fragments arranged by size:
5391 2064 616 393 260 162 25 18
PshAI GACnn'nnGTC
Cuts at: 1613 2102 1613
Size: 489 8440
PsiI TTA'TAA
Cuts at: 6499 6499
Size: 8929
PspOMI G'GGCC_C
Cuts at: 3744 5229 5729 3744
Size: 1485 500 6944
Fragments arranged by size:
6944 1485 500
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2396 2428 2396
Size: 32 8897
PstI C_TGCA'G
Cuts at: 830 2121 3192 3358 4782 5059 5662 6281 830
Size: 1291 1071 166 1424 277 603 619 3478
Fragments arranged by size:
3478 1424 1291 1071 619 603 277 166
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8929
PvuII CAG'CTG
Cuts at: 2452 4445 4587 4933 5068 2452
Size: 1993 142 346 135 6313
Fragments arranged by size:
6313 1993 346 142 135
RsrII CG'GwC_CG
Cuts at: 5532 5532
Size: 8929
SacI G_AGCT'C
Cuts at: 3602 5722 3602
Size: 2120 6809
SacII CC_GC'GG
Cuts at: 5244 5244
Size: 8929
SalI G'TCGA_C
Cuts at: 1936 6271 1936
Size: 4335 4594
SanDI GG'GwC_CC
Cuts at: 4453 5462 4453
Size: 1009 7920
SapI GCTCTTCn'nnn_
Cuts at: 3719 4721 3719
Size: 1002 7927
SbfI CC_TGCA'GG
Cuts at: 4782 6281 4782
Size: 1499 7430
ScaI AGT'ACT
Cuts at: 7177 8889 7177
Size: 1712 7217
SexAI A'CCwGG_T
Cuts at: 2862 2862
Size: 8929
SfcI C'TryA_G
Cuts at: 826 1045 2117 2495 3188 3354 4311 4778 5055
Size: 219 1072 378 693 166 957 467 277
Cuts at: 5055 5248 5658 6277 7412 8093 8284 826
Size: 193 410 619 1135 681 191 1471
Fragments arranged by size:
1471 1135 1072 957 693 681 619 467
410 378 277 219 193 191 166
SfoI GGC'GCC
Cuts at: 3871 5025 3871
Size: 1154 7775
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8929
SgrAI Cr'CCGG_yG
Cuts at: 1700 3866 1700
Size: 2166 6763
SmaI CCC'GGG
Cuts at: 1872 2444 3986 4307 5445 6256 1872
Size: 572 1542 321 1138 811 4545
Fragments arranged by size:
4545 1542 1138 811 572 321
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3995 7033 7934 8196 820
Size: 197 926 2052 3038 901 262 1553
Fragments arranged by size:
3038 2052 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6226 6720 493
Size: 5733 494 2702
Fragments arranged by size:
5733 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8929
SphI G_CATG'C
Cuts at: 1132 3415 6287 1132
Size: 2283 2872 3774
Fragments arranged by size:
3774 2872 2283
SspI AAT'ATT
Cuts at: 5 52 6853 5
Size: 47 6801 2081
Fragments arranged by size:
6801 2081 47
StuI AGG'CCT
Cuts at: 1517 5846 5953 1517
Size: 4329 107 4493
Fragments arranged by size:
4493 4329 107
StyI C'CwwG_G
Cuts at: 513 1065 2100 2883 3105 4870 6368 513
Size: 552 1035 783 222 1765 1498 3074
Fragments arranged by size:
3074 1765 1498 1035 783 552 222
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3547 4192 4333 4784 5163 6044 6956
Size: 1255 1967 645 141 451 379 881 912
Cuts at: 6956 6973 7126 7311 7756 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1967 1498 1255 912 881 645 451 445
379 185 153 141 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2091 7175 8887
Size: 274 80 33 51 1443 114 5084 1712
Cuts at: 8887 96
Size: 138
Fragments arranged by size:
5084 1712 1443 274 138 114 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2438 2470 2780 2812 4574 4606 4983 5015 5704
Size: 32 310 32 1762 32 377 32 689
Cuts at: 5704 5736 5861 5893 8889 8921 2438
Size: 32 125 32 2996 32 2446
Fragments arranged by size:
2996 2446 1762 689 377 310 125 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2612 5044 1316
Size: 1296 2432 5201
Fragments arranged by size:
5201 2432 1296
XbaI T'CTAG_A
Cuts at: 6265 6265
Size: 8929
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2593 3305 3463 3910 4034 4052 4522 4717 5936
Size: 712 158 447 124 18 470 195 1219
Cuts at: 5936 2593
Size: 5586
Fragments arranged by size:
5586 1219 712 470 447 195 158 124
18
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8929
XmaI C'CCGG_G
Cuts at: 1870 2442 3984 4305 5443 6254 1870
Size: 572 1542 321 1138 811 4545
Fragments arranged by size:
4545 1542 1138 811 572 321
XmnI GAAnn'nnTTC
Cuts at: 7058 7058
Size: 8929
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2854 6737 276
Size: 53 83 186 936 1320 3883 2468
Fragments arranged by size:
3883 2468 1320 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BstZ17I EcoRV
FspAI NruI NsiI PacI PmlI PspXI SfiI SrfI
SwaI