Restriction map for
pFN21ASDA1074
(Circular) (Six-base) MAPSORT of: pFN21ASDA1074.seq Check: 1235 from: 1 to: 9856
pFN21ASDA1074 9856 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7666 278
Size: 53 83 186 936 6130 2468
Fragments arranged by size:
6130 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6280 7064 7199 1937
Size: 4343 784 135 4594
Fragments arranged by size:
4594 4343 784 135
Acc65I G'GTAC_C
Cuts at: 7177 7177
Size: 9856
AclI AA'CG_TT
Cuts at: 1563 7983 8356 1563
Size: 6420 373 3063
Fragments arranged by size:
6420 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2766 3759 4871 5948 6015 7919 9303 9571
Size: 1134 993 1112 1077 67 1904 1384 268
Cuts at: 9571 1632
Size: 1917
Fragments arranged by size:
1917 1904 1384 1134 1112 1077 993 268
67
AflII C'TTAA_G
Cuts at: 820 1017 2755 820
Size: 197 1738 7921
Fragments arranged by size:
7921 1738 197
AflIII A'CryG_T
Cuts at: 1203 8755 9732 1203
Size: 7552 977 1327
Fragments arranged by size:
7552 1327 977
AgeI A'CCGG_T
Cuts at: 8687 8687
Size: 9856
AhdI GACnn_n'nnGTC
Cuts at: 8585 8585
Size: 9856
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9856
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9856
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4512 4544 5103 5135 4512
Size: 32 559 32 9233
Fragments arranged by size:
9233 559 32 32
AlwNI CAG_nnn'CTG
Cuts at: 5858 6043 7599 9171 5858
Size: 185 1556 1572 6543
Fragments arranged by size:
6543 1572 1556 185
ApaLI G'TGCA_C
Cuts at: 3209 7913 9069 3209
Size: 4704 1156 3996
Fragments arranged by size:
4704 3996 1156
ApoI r'AATT_y
Cuts at: 1493 2609 2893 3304 3907 4138 4650 4708 4717
Size: 1116 284 411 603 231 512 58 9
Cuts at: 4717 4984 5164 5986 6161 6303 7165 7391 7543
Size: 267 180 822 175 142 862 226 152
Cuts at: 7543 9652 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1116 862 822 603 512 411
284 267 231 226 180 175 152 142
58 9
AseI AT'TA_AT
Cuts at: 160 1032 3764 5514 5769 6216 8410 160
Size: 872 2732 1750 255 447 2194 1606
Fragments arranged by size:
2732 2194 1750 1606 872 447 255
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9856
AvaI C'yCGr_G
Cuts at: 1870 1943 4617 7181 1870
Size: 73 2674 2564 4545
Fragments arranged by size:
4545 2674 2564 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5345 5378 5345
Size: 33 9823
BamHI G'GATC_C
Cuts at: 7186 7553 7186
Size: 367 9489
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2120 7177 8632 618
Size: 325 217 627 333 5057 1455 1842
Fragments arranged by size:
5057 1842 1455 627 333 325 217
BanII G_rGCy'C
Cuts at: 1391 2066 7175 1391
Size: 675 5109 4072
Fragments arranged by size:
5109 4072 675
BbeI G_GCGC'C
Cuts at: 2124 2124
Size: 9856
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2754 2820 4296 4887 4898 5510 5570
Size: 971 855 66 1476 591 11 612 60
Cuts at: 5570 6660 928
Size: 1090 4124
Fragments arranged by size:
4124 1476 1090 971 855 612 591 66
60 11
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4365 4399 4598 4632 8047 8081 1903
Size: 34 2428 34 199 34 3415 34 3678
Fragments arranged by size:
3678 3415 2428 199 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2691 3442 6283 7748 8958 2691
Size: 751 2841 1465 1210 3589
Fragments arranged by size:
3589 2841 1465 1210 751
BclI T'GATC_A
Cuts at: 1548 3793 5080 6393 1548
Size: 2245 1287 1313 5011
Fragments arranged by size:
5011 2245 1313 1287
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8467 136
Size: 107 122 71 631 605 6795 1525
Fragments arranged by size:
6795 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2158 6613 6803 6877 7090 9851 2158
Size: 4455 190 74 213 2761 2163
Fragments arranged by size:
4455 2761 2163 213 190 74
BlpI GC'TnA_GC
Cuts at: 2854 7273 2854
Size: 4419 5437
Bme1580I G_kGCm'C
Cuts at: 1790 3213 7917 9073 1790
Size: 1423 4704 1156 2573
Fragments arranged by size:
4704 2573 1423 1156
BmgBI CAC'GTC
Cuts at: 1318 2146 1318
Size: 828 9028
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4930 7587 8545
Size: 416 53 83 399 24 3513 2657 958
Cuts at: 8545 442
Size: 1753
Fragments arranged by size:
3513 2657 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 6761 1056
Size: 5705 4151
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3313 5294 5445 3313
Size: 1981 151 7724
Fragments arranged by size:
7724 1981 151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4559 5015 5765 6278 8516 1239
Size: 3320 456 750 513 2238 2579
Fragments arranged by size:
3320 2579 2238 750 513 456
Bpu10I CC'TnA_GC
Cuts at: 2225 5321 2225
Size: 3096 6760
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6525 6633 6985 7981 8846 9144 6525
Size: 108 352 996 865 298 7237
Fragments arranged by size:
7237 996 865 352 298 108
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2145 8519 882
Size: 617 646 6374 2219
Fragments arranged by size:
6374 2219 646 617
BsaAI yAC'GTr
Cuts at: 493 1204 7153 7647 493
Size: 711 5949 494 2702
Fragments arranged by size:
5949 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 2439 5079 5516 9850 2439
Size: 2640 437 4334 2445
Fragments arranged by size:
4334 2640 2445 437
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2121 7663 8045 8680
Size: 53 83 186 936 588 5542 382 635
Cuts at: 8680 275
Size: 1451
Fragments arranged by size:
5542 1451 936 635 588 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 8289 8687 8961 9108 9519 8289
Size: 398 274 147 411 8626
Fragments arranged by size:
8626 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4512 4542 5411 5441 5738
Size: 30 165 30 3109 30 869 30 297
Cuts at: 5738 5768 1178
Size: 30 5266
Fragments arranged by size:
5266 3109 869 297 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2098 5432 5972 6329 1186
Size: 912 3334 540 357 4713
Fragments arranged by size:
4713 3334 912 540 357
BseYI C'CCAG_C
Cuts at: 3335 9059 9555 3335
Size: 5724 496 3636
Fragments arranged by size:
5724 3636 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2228 1163
Size: 573 492 8791
Fragments arranged by size:
8791 573 492
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7306 8067 9095 1540
Size: 275 186 5305 761 1028 2301
Fragments arranged by size:
5305 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3163 3213 3872 4388 7917 8002 9073 3163
Size: 50 659 516 3529 85 1071 3946
Fragments arranged by size:
3946 3529 1071 659 516 85 50
BsmI GAATG_Cn'
Cuts at: 4633 4978 7367 7460 4633
Size: 345 2389 93 7029
Fragments arranged by size:
7029 2389 345 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 3163 3213 3872 4388 7175 7917
Size: 399 276 1097 50 659 516 2787 742
Cuts at: 7917 8002 9073 1391
Size: 85 1071 2174
Fragments arranged by size:
2787 2174 1097 1071 742 659 516 399
276 85 50
BspEI T'CCGG_A
Cuts at: 9519 9519
Size: 9856
BspHI T'CATG_A
Cuts at: 4729 7745 9475 4729
Size: 3016 1730 5110
Fragments arranged by size:
5110 3016 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6257 7211 844
Size: 5413 954 3489
Fragments arranged by size:
5413 3489 954
BsrBI CCG'CTC
Cuts at: 1394 1823 7743 1394
Size: 429 5920 3507
Fragments arranged by size:
5920 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3120 4983 8351 8525 65
Size: 1183 1872 1863 3368 174 1396
Fragments arranged by size:
3368 1872 1863 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 8500 8687 1700
Size: 110 6690 187 2869
Fragments arranged by size:
6690 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2383 3568 7120 96
Size: 2287 1185 3552 2832
Fragments arranged by size:
3552 2832 2287 1185
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9856
BssSI C'ACGA_G
Cuts at: 5266 7916 8928 5266
Size: 2650 1012 6194
Fragments arranged by size:
6194 2650 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9856
BstBI TT'CG_AA
Cuts at: 2007 8660 2007
Size: 6653 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9856
BstXI CCAn_nnnn'nTGG
Cuts at: 4943 4943
Size: 9856
BstYI r'GATC_y
Cuts at: 1972 2158 2169 2214 6613 6803 6877 7090 7186
Size: 186 11 45 4399 190 74 213 96
Cuts at: 7186 7553 7938 7955 9407 9493 9505 9851 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
4399 1977 1452 385 367 346 213 190
186 96 86 74 45 17 12 11
BtgI C'CryG_G
Cuts at: 513 1065 2455 6172 6932 513
Size: 552 1390 3717 760 3437
Fragments arranged by size:
3717 3437 1390 760 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9117
Fragments arranged by size:
9117 683 56
BtsI GCAGTG_nn'
Cuts at: 2463 7372 8166 8186 2463
Size: 4909 794 20 4133
Fragments arranged by size:
4909 4133 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9856
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9821
DraI TTT'AAA
Cuts at: 5221 5796 6183 7131 7160 5221
Size: 575 387 948 29 7917
Fragments arranged by size:
7917 948 575 387 29
DraIII CAC_nnn'GTG
Cuts at: 6851 6851
Size: 9856
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8863 809
Size: 507 7547 1802
Fragments arranged by size:
7547 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 7303 8192 9821
Size: 54 1425 50 275 18 5473 889 1629
Cuts at: 9821 8
Size: 43
Fragments arranged by size:
5473 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 7303 1537
Size: 275 5491 4090
Fragments arranged by size:
5491 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2391 2475 3612 4410 4415 4473 7786 1353
Size: 1038 84 1137 798 5 58 3313 3423
Fragments arranged by size:
3423 3313 1137 1038 798 84 58 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2095 8443 8817 8963 387
Size: 1064 138 506 6348 374 146 1280
Fragments arranged by size:
6348 1280 1064 506 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2766 3759 4559 4871 5015 5765 5948
Size: 393 1134 993 800 312 144 750 183
Cuts at: 5948 6015 6278 7919 8516 9303 9571 1239
Size: 67 263 1641 597 787 268 1524
Fragments arranged by size:
1641 1524 1134 993 800 787 750 597
393 312 268 263 183 144 67
EcoNI CCTnn'n_nnAGG
Cuts at: 2758 2758
Size: 9856
EcoO109I rG'GnC_Cy
Cuts at: 4852 5317 4852
Size: 465 9391
EcoRI G'AATT_C
Cuts at: 3907 4650 4708 4717 7165 7543 3907
Size: 743 58 9 2448 378 6220
Fragments arranged by size:
6220 2448 743 378 58 9
EcoRV GAT'ATC
Cuts at: 3500 3500
Size: 9856
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2254 6133 2254
Size: 3879 5977
FspI TGC'GCA
Cuts at: 8362 8362
Size: 9856
HaeII r_GCGC'y
Cuts at: 1678 2124 9003 1678
Size: 446 6879 2531
Fragments arranged by size:
6879 2531 446
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2483 2515 2726 2758 2860
Size: 32 135 32 911 32 211 32 102
Cuts at: 2860 2892 3221 3253 3257 3289 5191 5223 5411
Size: 32 329 32 4 32 1902 32 188
Cuts at: 5411 5443 5738 5770 5869 5901 6226 6258 6308
Size: 32 295 32 99 32 325 32 50
Cuts at: 6308 6340 6605 6637 8497 8529 8571 8603 1373
Size: 32 265 32 1860 32 42 32 2626
Fragments arranged by size:
2626 1902 1860 911 329 325 295 265
211 188 135 102 99 50 42 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 4
HincII GTy'rAC
Cuts at: 669 1657 1938 6769 7200 7446 669
Size: 988 281 4831 431 246 3079
Fragments arranged by size:
4831 3079 988 431 281 246
HindIII A'AGCT_T
Cuts at: 748 2851 5897 748
Size: 2103 3046 4707
Fragments arranged by size:
4707 3046 2103
HpaI GTT'AAC
Cuts at: 7446 7446
Size: 9856
KasI G'GCGC_C
Cuts at: 2120 2120
Size: 9856
KpnI G_GTAC'C
Cuts at: 7181 7181
Size: 9856
MfeI C'AATT_G
Cuts at: 5825 7455 5825
Size: 1630 8226
MluI A'CGCG_T
Cuts at: 9732 9732
Size: 9856
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2634 6360 6966 8970 9154 1305
Size: 1329 3726 606 2004 184 2007
Fragments arranged by size:
3726 2007 2004 1329 606 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 8034
Fragments arranged by size:
8034 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3524 3720 4728 5076 6046 7814 8173
Size: 648 2358 196 1008 348 970 1768 359
Cuts at: 8173 8332 518
Size: 159 2042
Fragments arranged by size:
2358 2042 1768 1008 970 648 359 348
196 159
MspA1I CmG'CkG
Cuts at: 1606 3587 4967 7272 7949 9097 9342 1606
Size: 1981 1380 2305 677 1148 245 2120
Fragments arranged by size:
2305 2120 1981 1380 1148 677 245
NarI GG'CG_CC
Cuts at: 2121 2121
Size: 9856
NcoI C'CATG_G
Cuts at: 513 1065 2455 6172 513
Size: 552 1390 3717 4197
Fragments arranged by size:
4197 3717 1390 552
NdeI CA'TA_TG
Cuts at: 387 2908 387
Size: 2521 7335
NheI G'CTAG_C
Cuts at: 1052 6757 1052
Size: 5705 4151
NotI GC'GGCC_GC
Cuts at: 7303 7303
Size: 9856
NspI r_CATG'y
Cuts at: 1132 1229 3469 4768 7214 8759 1132
Size: 97 2240 1299 2446 1545 2229
Fragments arranged by size:
2446 2240 2229 1545 1299 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9856
PciI A'CATG_T
Cuts at: 8755 8755
Size: 9856
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3920 4537 1108
Size: 2812 617 6427
Fragments arranged by size:
6427 2812 617
PfoI T'CCnGG_A
Cuts at: 2885 4203 2885
Size: 1318 8538
PmeI GTTT'AAAC
Cuts at: 7160 7160
Size: 9856
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4512 4544 7925 7957 9438 9470 4512
Size: 32 3381 32 1481 32 4898
Fragments arranged by size:
4898 3381 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4852 5317 4852
Size: 465 9391
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9856
PsiI TTA'TAA
Cuts at: 7426 7426
Size: 9856
PstI C_TGCA'G
Cuts at: 830 3346 6268 7208 830
Size: 2516 2922 940 3478
Fragments arranged by size:
3478 2922 2516 940
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9856
PvuII CAG'CTG
Cuts at: 3587 4967 3587
Size: 1380 8476
SalI G'TCGA_C
Cuts at: 1936 7198 1936
Size: 5262 4594
SapI GCTCTTCn'nnn_
Cuts at: 2475 3612 4473 2475
Size: 1137 861 7858
Fragments arranged by size:
7858 1137 861
SbfI CC_TGCA'GG
Cuts at: 7208 7208
Size: 9856
ScaI AGT'ACT
Cuts at: 8104 9816 8104
Size: 1712 8144
SfcI C'TryA_G
Cuts at: 826 1045 3342 5412 6264 6359 7204 8339 9020
Size: 219 2297 2070 852 95 845 1135 681
Cuts at: 9020 9211 826
Size: 191 1471
Fragments arranged by size:
2297 2070 1471 1135 852 845 681 219
191 95
SfoI GGC'GCC
Cuts at: 2122 2122
Size: 9856
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9856
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9856
SmaI CCC'GGG
Cuts at: 1872 7183 1872
Size: 5311 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2755 4617 6540 6648 6964 7960
Size: 197 926 812 1862 1923 108 316 996
Cuts at: 7960 8861 9123 820
Size: 901 262 1553
Fragments arranged by size:
1923 1862 1553 996 926 901 812 316
262 197 108
SnaBI TAC'GTA
Cuts at: 493 7153 7647 493
Size: 6660 494 2702
Fragments arranged by size:
6660 2702 494
SpeI A'CTAG_T
Cuts at: 152 6188 152
Size: 6036 3820
SphI G_CATG'C
Cuts at: 1132 3469 7214 1132
Size: 2337 3745 3774
Fragments arranged by size:
3774 3745 2337
SspI AAT'ATT
Cuts at: 5 52 2536 7780 5
Size: 47 2484 5244 2081
Fragments arranged by size:
5244 2484 2081 47
StuI AGG'CCT
Cuts at: 1517 4006 1517
Size: 2489 7367
StyI C'CwwG_G
Cuts at: 513 1065 2058 2455 3278 6172 7295 513
Size: 552 993 397 823 2894 1123 3074
Fragments arranged by size:
3074 2894 1123 993 823 552 397
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2169 7883 7900 8053 8238 8683 325
Size: 1255 589 5714 17 153 185 445 1498
Fragments arranged by size:
5714 1498 1255 589 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2383 3568 4303
Size: 274 80 33 51 1443 406 1185 735
Cuts at: 4303 6337 7120 8102 9814 96
Size: 2034 783 982 1712 138
Fragments arranged by size:
2034 1712 1443 1185 982 783 735 406
274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 9816 9848 9816
Size: 32 9824
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9856
XbaI T'CTAG_A
Cuts at: 5051 6697 7192 5051
Size: 1646 495 7715
Fragments arranged by size:
7715 1646 495
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6781 6835 6781
Size: 54 9802
XhoI C'TCGA_G
Cuts at: 1943 4617 1943
Size: 2674 7182
XmaI C'CCGG_G
Cuts at: 1870 7181 1870
Size: 5311 4545
XmnI GAAnn'nnTTC
Cuts at: 2988 4884 7985 2988
Size: 1896 3101 4859
Fragments arranged by size:
4859 3101 1896
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7664 276
Size: 53 83 186 936 6130 2468
Fragments arranged by size:
6130 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbvCI BfrBI BsiWI
BsmBI BstZ17I Bsu36I EcoICRI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SrfI SwaI