Restriction map for
pFN21ASDA1058
(Circular) (Six-base) MAPSORT of: pFN21ASDA1058.seq Check: 2015 from: 1 to: 10933
pFN21ASDA1058 10933 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8743 278
Size: 53 83 186 936 7207 2468
Fragments arranged by size:
7207 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2496 3273 4484 6324 8276 1937
Size: 559 777 1211 1840 1952 4594
Fragments arranged by size:
4594 1952 1840 1211 777 559
Acc65I G'GTAC_C
Cuts at: 8254 8254
Size: 10933
AclI AA'CG_TT
Cuts at: 1563 9060 9433 1563
Size: 7497 373 3063
Fragments arranged by size:
7497 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3452 3563 4910 6054 7057 8996 10380 10648
Size: 1820 111 1347 1144 1003 1939 1384 268
Cuts at: 10648 1632
Size: 1917
Fragments arranged by size:
1939 1917 1820 1384 1347 1144 1003 268
111
AfeI AGC'GCT
Cuts at: 7208 7208
Size: 10933
AflII C'TTAA_G
Cuts at: 820 1017 4061 4577 6385 8066 820
Size: 197 3044 516 1808 1681 3687
Fragments arranged by size:
3687 3044 1808 1681 516 197
AflIII A'CryG_T
Cuts at: 1203 4770 6438 9832 10809 1203
Size: 3567 1668 3394 977 1327
Fragments arranged by size:
3567 3394 1668 1327 977
AgeI A'CCGG_T
Cuts at: 9764 9764
Size: 10933
AhdI GACnn_n'nnGTC
Cuts at: 9662 9662
Size: 10933
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 10933
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4463 6943 1184
Size: 3279 2480 5174
Fragments arranged by size:
5174 3279 2480
AlwNI CAG_nnn'CTG
Cuts at: 2215 2312 6115 6277 6658 8676 10248 2215
Size: 97 3803 162 381 2018 1572 2900
Fragments arranged by size:
3803 2900 2018 1572 381 162 97
ApaI G_GGCC'C
Cuts at: 2141 2141
Size: 10933
ApaLI G'TGCA_C
Cuts at: 8990 10146 8990
Size: 1156 9777
ApoI r'AATT_y
Cuts at: 1493 2662 3890 4025 4145 4455 5219 5878 7500
Size: 1169 1228 135 120 310 764 659 1622
Cuts at: 7500 7535 8242 8468 8620 10729 1493
Size: 35 707 226 152 2109 1697
Fragments arranged by size:
2109 1697 1622 1228 1169 764 707 659
310 226 152 135 120 35
AseI AT'TA_AT
Cuts at: 160 1032 9487 160
Size: 872 8455 1606
Fragments arranged by size:
8455 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10933
AvaI C'yCGr_G
Cuts at: 1870 1943 2134 4695 6851 7339 8147 8258 1870
Size: 73 191 2561 2156 488 808 111 4545
Fragments arranged by size:
4545 2561 2156 808 488 191 111 73
BamHI G'GATC_C
Cuts at: 2648 3120 6888 8263 8630 2648
Size: 472 3768 1375 367 4951
Fragments arranged by size:
4951 3768 1375 472 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5702 8254 9709 618
Size: 325 217 627 3915 2552 1455 1842
Fragments arranged by size:
3915 2552 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2135 2141 2772 3350 4546 6494 6858 7170
Size: 744 6 631 578 1196 1948 364 312
Cuts at: 7170 7218 7905 8252 1391
Size: 48 687 347 4072
Fragments arranged by size:
4072 1948 1196 744 687 631 578 364
347 312 48 6
BbeI G_GCGC'C
Cuts at: 5706 5706
Size: 10933
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2210 4053 5095 5137 6367 6869 7125
Size: 971 311 1843 1042 42 1230 502 256
Cuts at: 7125 8145 928
Size: 1020 3716
Fragments arranged by size:
3716 1843 1230 1042 1020 971 502 311
256 42
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2280 2314 7202 7236 9124 9158 1903
Size: 34 343 34 4888 34 1888 34 3678
Fragments arranged by size:
4888 3678 1888 343 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3402 6738 8219 8825 10035 3402
Size: 3336 1481 606 1210 4300
Fragments arranged by size:
4300 3336 1481 1210 606
BclI T'GATC_A
Cuts at: 1548 4920 7938 1548
Size: 3372 3018 4543
Fragments arranged by size:
4543 3372 3018
BfrBI ATG'CAT
Cuts at: 3626 6097 8212 3626
Size: 2471 2115 6347
Fragments arranged by size:
6347 2471 2115
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3557 6953 7044
Size: 107 122 71 631 605 1885 3396 91
Cuts at: 7044 9544 136
Size: 2500 1525
Fragments arranged by size:
3396 2500 1885 1525 631 605 122 107
91 71
BglII A'GATC_T
Cuts at: 2682 10928 2682
Size: 8246 2687
BlpI GC'TnA_GC
Cuts at: 3078 3492 3747 4782 4935 6488 8350 3078
Size: 414 255 1035 153 1553 1862 5661
Fragments arranged by size:
5661 1862 1553 1035 414 255 153
Bme1580I G_kGCm'C
Cuts at: 1790 2141 3900 8994 10150 1790
Size: 351 1759 5094 1156 2573
Fragments arranged by size:
5094 2573 1759 1156 351
BmgBI CAC'GTC
Cuts at: 1318 3315 6867 7123 7630 1318
Size: 1997 3552 256 507 4621
Fragments arranged by size:
4621 3552 1997 507 256
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3749 4714 8664
Size: 416 53 83 399 24 2332 965 3950
Cuts at: 8664 9622 442
Size: 958 1753
Fragments arranged by size:
3950 2332 1753 965 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10933
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4066 5799 5801 7123 4066
Size: 1733 2 1322 7876
Fragments arranged by size:
7876 1733 1322 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2843 4560 5205 5297 5381 6800 7943 9593
Size: 1604 1717 645 92 84 1419 1143 1650
Cuts at: 9593 1239
Size: 2579
Fragments arranged by size:
2579 1717 1650 1604 1419 1143 645 92
84
Bpu10I CC'TnA_GC
Cuts at: 2109 6857 8110 2109
Size: 4748 1253 4932
Fragments arranged by size:
4932 4748 1253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2756 3344 3692 4222 4771 4835 5423 6452 7193
Size: 588 348 530 549 64 588 1029 741
Cuts at: 7193 9058 9923 10221 2756
Size: 1865 865 298 3468
Fragments arranged by size:
3468 1865 1029 865 741 588 588 549
530 348 298 64
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4354 5181 9596 882
Size: 617 2855 827 4415 2219
Fragments arranged by size:
4415 2855 2219 827 617
BsaAI yAC'GTr
Cuts at: 493 1204 3271 4606 6940 8230 8724 493
Size: 711 2067 1335 2334 1290 494 2702
Fragments arranged by size:
2702 2334 2067 1335 1290 711 494
BsaBI GATnn'nnATC
Cuts at: 5426 10927 5426
Size: 5501 5432
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5651 5703 8740 9122
Size: 53 83 186 936 4118 52 3037 382
Cuts at: 9122 9757 275
Size: 635 1451
Fragments arranged by size:
4118 3037 1451 936 635 382 186 83
53 52
BsaWI w'CCGG_w
Cuts at: 2948 3246 4414 6573 9366 9764 10038 10185 10596
Size: 298 1168 2159 2793 398 274 147 411
Cuts at: 10596 2948
Size: 3285
Fragments arranged by size:
3285 2793 2159 1168 411 398 298 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5725 5755 1178
Size: 30 165 30 4322 30 6356
Fragments arranged by size:
6356 4322 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2504 6706 7045 7048 7115 7909 1186
Size: 1318 4202 339 3 67 794 4210
Fragments arranged by size:
4210 4202 1318 794 339 67 3
BseYI C'CCAG_C
Cuts at: 5698 6115 6513 8221 10136 10632 5698
Size: 417 398 1708 1915 496 5999
Fragments arranged by size:
5999 1915 1708 496 417 398
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5669 6029 6164 7286 1163
Size: 573 3933 360 135 1122 4810
Fragments arranged by size:
4810 3933 1122 573 360 135
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7926 8383 9144 10172 1540
Size: 275 186 5925 457 761 1028 2301
Fragments arranged by size:
5925 2301 1028 761 457 275 186
BsiHKAI G_wGCw'C
Cuts at: 2772 5439 6494 6858 7170 7218 7905 7919 8994
Size: 2667 1055 364 312 48 687 14 1075
Cuts at: 8994 9079 10150 2772
Size: 85 1071 3555
Fragments arranged by size:
3555 2667 1075 1071 1055 687 364 312
85 48 14
BsmI GAATG_Cn'
Cuts at: 3553 3591 5458 8444 8537 3553
Size: 38 1867 2986 93 5949
Fragments arranged by size:
5949 2986 1867 93 38
BsmBI CGTCTCn'nnnn_
Cuts at: 2272 2608 2272
Size: 336 10597
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2135 2141 2772 3350 3900 4546 5439
Size: 399 345 6 631 578 550 646 893
Cuts at: 5439 6494 6858 7170 7218 7905 7919 8252 8994
Size: 1055 364 312 48 687 14 333 742
Cuts at: 8994 9079 10150 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 1055 893 742 687 646 631
578 550 399 364 345 333 312 85
48 14 6
BspEI T'CCGG_A
Cuts at: 4414 6573 10596 4414
Size: 2159 4023 4751
Fragments arranged by size:
4751 4023 2159
BspHI T'CATG_A
Cuts at: 3373 6303 8822 10552 3373
Size: 2930 2519 1730 3754
Fragments arranged by size:
3754 2930 2519 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2973 3863 5454 5526 5703 8288 844
Size: 2129 890 1591 72 177 2585 3489
Fragments arranged by size:
3489 2585 2129 1591 890 177 72
BsrBI CCG'CTC
Cuts at: 1394 1823 6434 8820 1394
Size: 429 4611 2386 3507
Fragments arranged by size:
4611 3507 2386 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2886 3142 3164 3484 5715 6204 6930
Size: 1183 1638 256 22 320 2231 489 726
Cuts at: 6930 7218 9428 9602 65
Size: 288 2210 174 1396
Fragments arranged by size:
2231 2210 1638 1396 1183 726 489 320
288 256 174 22
BsrFI r'CCGG_y
Cuts at: 1700 1810 2146 6690 9577 9764 1700
Size: 110 336 4544 2887 187 2869
Fragments arranged by size:
4544 2887 2869 336 187 110
BsrGI T'GTAC_A
Cuts at: 96 2562 96
Size: 2466 8467
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10933
BssSI C'ACGA_G
Cuts at: 8993 10005 8993
Size: 1012 9921
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 10933
BstBI TT'CG_AA
Cuts at: 2007 9737 2007
Size: 7730 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 10933
BstXI CCAn_nnnn'nTGG
Cuts at: 2537 3597 4391 4468 6155 7867 2537
Size: 1060 794 77 1687 1712 5603
Fragments arranged by size:
5603 1712 1687 1060 794 77
BstYI r'GATC_y
Cuts at: 1972 2211 2648 2682 2853 3120 5780 6888 7591
Size: 239 437 34 171 267 2660 1108 703
Cuts at: 7591 7685 8263 8630 9015 9032 10484 10570 10582
Size: 94 578 367 385 17 1452 86 12
Cuts at: 10582 10928 1972
Size: 346 1977
Fragments arranged by size:
2660 1977 1452 1108 703 578 437 385
367 346 267 239 171 94 86 34
17 12
BstZ17I GTA'TAC
Cuts at: 4485 4485
Size: 10933
Bsu36I CC'TnA_GG
Cuts at: 4552 7028 7587 4552
Size: 2476 559 7898
Fragments arranged by size:
7898 2476 559
BtgI C'CryG_G
Cuts at: 513 1065 5883 6286 513
Size: 552 4818 403 5160
Fragments arranged by size:
5160 4818 552 403
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2658 4607 487
Size: 683 56 1432 1949 6813
Fragments arranged by size:
6813 1949 1432 683 56
BtsI GCAGTG_nn'
Cuts at: 3343 4445 5013 6378 7184 7769 7795 8449 9243
Size: 1102 568 1365 806 585 26 654 794
Cuts at: 9243 9263 3343
Size: 20 5013
Fragments arranged by size:
5013 1365 1102 806 794 654 585 568
26 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10933
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10898
DraI TTT'AAA
Cuts at: 6366 8237 6366
Size: 1871 9062
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2286 2492 2602 9940 809
Size: 507 970 206 110 7338 1802
Fragments arranged by size:
7338 1802 970 507 206 110
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2268 3447 6157
Size: 54 1425 50 275 18 438 1179 2710
Cuts at: 6157 6279 6762 6828 6882 7869 7923 8380 9269
Size: 122 483 66 54 987 54 457 889
Cuts at: 9269 10898 8
Size: 1629 43
Fragments arranged by size:
2710 1629 1425 1179 987 889 483 457
438 275 122 66 54 54 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7923 8380 1537
Size: 275 6111 457 4090
Fragments arranged by size:
6111 4090 457 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2249 2319 3096 3523 4253 5828 7733 7752
Size: 896 70 777 427 730 1575 1905 19
Cuts at: 7752 8863 1353
Size: 1111 3423
Fragments arranged by size:
3423 1905 1575 1111 896 777 730 427
70 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2336 7688 7913 9520 9894 10040
Size: 1064 138 747 5352 225 1607 374 146
Cuts at: 10040 387
Size: 1280
Fragments arranged by size:
5352 1607 1280 1064 747 374 225 146
138
EcoICRI GAG'CTC
Cuts at: 2770 6492 6856 7168 7216 7903 2770
Size: 3722 364 312 48 687 5800
Fragments arranged by size:
5800 3722 687 364 312 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2843 3452 3563 4560 4910 5205 5297
Size: 393 1211 609 111 997 350 295 92
Cuts at: 5297 5381 6054 6800 7057 7943 8996 9593 10380
Size: 84 673 746 257 886 1053 597 787
Cuts at: 10380 10648 1239
Size: 268 1524
Fragments arranged by size:
1524 1211 1053 997 886 787 746 673
609 597 393 350 295 268 257 111
92 84
EcoNI CCTnn'n_nnAGG
Cuts at: 5687 7027 5687
Size: 1340 9593
EcoO109I rG'GnC_Cy
Cuts at: 2138 3185 5692 6390 7359 2138
Size: 1047 2507 698 969 5712
Fragments arranged by size:
5712 2507 1047 969 698
EcoRI G'AATT_C
Cuts at: 4025 8242 8620 4025
Size: 4217 378 6338
Fragments arranged by size:
6338 4217 378
EcoRV GAT'ATC
Cuts at: 8042 8042
Size: 10933
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3082 5218 6583 3082
Size: 2136 1365 7432
Fragments arranged by size:
7432 2136 1365
FspI TGC'GCA
Cuts at: 7183 9439 7183
Size: 2256 8677
HaeII r_GCGC'y
Cuts at: 1678 3326 4110 5706 7023 7210 10080 1678
Size: 1648 784 1596 1317 187 2870 2531
Fragments arranged by size:
2870 2531 1648 1596 1317 784 187
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2501 2533 3056 3088 9574
Size: 32 135 32 929 32 523 32 6486
Cuts at: 9574 9606 9648 9680 1373
Size: 32 42 32 2626
Fragments arranged by size:
6486 2626 929 523 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2485 2497 4687 7582 8277 8523
Size: 988 281 547 12 2190 2895 695 246
Cuts at: 8523 669
Size: 3079
Fragments arranged by size:
3079 2895 2190 988 695 547 281 246
12
HindIII A'AGCT_T
Cuts at: 748 3059 8094 748
Size: 2311 5035 3587
Fragments arranged by size:
5035 3587 2311
HpaI GTT'AAC
Cuts at: 4687 8523 4687
Size: 3836 7097
KasI G'GCGC_C
Cuts at: 5702 5702
Size: 10933
KpnI G_GTAC'C
Cuts at: 8258 8258
Size: 10933
MfeI C'AATT_G
Cuts at: 3783 8532 3783
Size: 4749 6184
MluI A'CGCG_T
Cuts at: 4770 10809 4770
Size: 6039 4894
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2433 7476 10047 10231 1305
Size: 1128 5043 2571 184 2007
Fragments arranged by size:
5043 2571 2007 1128 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3449 6159 6764 6830 6884
Size: 54 1425 343 1617 2710 605 66 54
Cuts at: 6884 7871 10
Size: 987 3072
Fragments arranged by size:
3072 2710 1617 1425 987 605 343 66
54 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2475 2591 2913 5126 5432 5603 6153
Size: 648 1309 116 322 2213 306 171 550
Cuts at: 6153 7290 7619 7865 8891 9250 9409 518
Size: 1137 329 246 1026 359 159 2042
Fragments arranged by size:
2213 2042 1309 1137 1026 648 550 359
329 322 306 246 171 159 116
MspA1I CmG'CkG
Cuts at: 1606 3077 4183 4227 4642 5554 5633 5644 6115
Size: 1471 1106 44 415 912 79 11 471
Cuts at: 6115 6517 6610 7492 7912 8221 8349 9026 10174
Size: 402 93 882 420 309 128 677 1148
Cuts at: 10174 10419 1606
Size: 245 2120
Fragments arranged by size:
2120 1471 1148 1106 912 882 677 471
420 415 402 309 245 128 93 79
44 11
NaeI GCC'GGC
Cuts at: 2148 2148
Size: 10933
NarI GG'CG_CC
Cuts at: 5703 5703
Size: 10933
NcoI C'CATG_G
Cuts at: 513 1065 6286 513
Size: 552 5221 5160
Fragments arranged by size:
5221 5160 552
NdeI CA'TA_TG
Cuts at: 387 2297 4759 4789 8002 387
Size: 1910 2462 30 3213 3318
Fragments arranged by size:
3318 3213 2462 1910 30
NgoMIV G'CCGG_C
Cuts at: 2146 2146
Size: 10933
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10933
NotI GC'GGCC_GC
Cuts at: 8380 8380
Size: 10933
NsiI A_TGCA'T
Cuts at: 3628 6099 8214 3628
Size: 2471 2115 6347
Fragments arranged by size:
6347 2471 2115
NspI r_CATG'y
Cuts at: 1132 1229 3564 3706 4592 5962 6101 6442 8291
Size: 97 2335 142 886 1370 139 341 1849
Cuts at: 8291 9836 1132
Size: 1545 2229
Fragments arranged by size:
2335 2229 1849 1545 1370 886 341 142
139 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10933
PciI A'CATG_T
Cuts at: 6438 9832 6438
Size: 3394 7539
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5359 6061 6277 1108
Size: 4251 702 216 5764
Fragments arranged by size:
5764 4251 702 216
PfoI T'CCnGG_A
Cuts at: 5182 5397 5406 5182
Size: 215 9 10709
Fragments arranged by size:
10709 215 9
PmeI GTTT'AAAC
Cuts at: 8237 8237
Size: 10933
PmlI CAC'GTG
Cuts at: 4606 4606
Size: 10933
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3272 3304 7467 7499 9002 9034 10515 10547 3272
Size: 32 4163 32 1503 32 1481 32 3658
Fragments arranged by size:
4163 3658 1503 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3185 5692 6390 7359 3185
Size: 2507 698 969 6759
Fragments arranged by size:
6759 2507 969 698
PshAI GACnn'nnGTC
Cuts at: 1613 4697 7328 7628 1613
Size: 3084 2631 300 4918
Fragments arranged by size:
4918 3084 2631 300
PsiI TTA'TAA
Cuts at: 2670 6402 8503 2670
Size: 3732 2101 5100
Fragments arranged by size:
5100 3732 2101
PspOMI G'GGCC_C
Cuts at: 2137 2137
Size: 10933
PspXI vC'TCGA_Gb
Cuts at: 8147 8147
Size: 10933
PstI C_TGCA'G
Cuts at: 830 3860 4120 5348 7950 8285 830
Size: 3030 260 1228 2602 335 3478
Fragments arranged by size:
3478 3030 2602 1228 335 260
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10933
PvuII CAG'CTG
Cuts at: 3077 4183 4227 4642 5633 6115 6517 6610 7912
Size: 1106 44 415 991 482 402 93 1302
Cuts at: 7912 8221 3077
Size: 309 5789
Fragments arranged by size:
5789 1302 1106 991 482 415 402 309
93 44
SacI G_AGCT'C
Cuts at: 2772 6494 6858 7170 7218 7905 2772
Size: 3722 364 312 48 687 5800
Fragments arranged by size:
5800 3722 687 364 312 48
SalI G'TCGA_C
Cuts at: 1936 2495 8275 1936
Size: 559 5780 4594
Fragments arranged by size:
5780 4594 559
SapI GCTCTTCn'nnn_
Cuts at: 2249 5828 7752 2249
Size: 3579 1924 5430
Fragments arranged by size:
5430 3579 1924
SbfI CC_TGCA'GG
Cuts at: 8285 8285
Size: 10933
ScaI AGT'ACT
Cuts at: 3965 4520 4895 9181 10893 3965
Size: 555 375 4286 1712 4005
Fragments arranged by size:
4286 4005 1712 555 375
SexAI A'CCwGG_T
Cuts at: 4081 4081
Size: 10933
SfcI C'TryA_G
Cuts at: 826 1045 3667 3856 4116 4476 5344 7312 7946
Size: 219 2622 189 260 360 868 1968 634
Cuts at: 7946 8176 8281 9416 10097 10288 826
Size: 230 105 1135 681 191 1471
Fragments arranged by size:
2622 1968 1471 1135 868 681 634 360
260 230 219 191 189 105
SfoI GGC'GCC
Cuts at: 5704 5704
Size: 10933
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10933
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10933
SmaI CCC'GGG
Cuts at: 1872 2136 8260 1872
Size: 264 6124 4545
Fragments arranged by size:
6124 4545 264
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2771 3359 3707 4061 4237 4577
Size: 197 926 828 588 348 354 176 340
Cuts at: 4577 4750 4850 5438 6385 6467 7172 8066 8147
Size: 173 100 588 947 82 705 894 81
Cuts at: 8147 9037 9938 10200 820
Size: 890 901 262 1553
Fragments arranged by size:
1553 947 926 901 894 890 828 705
588 588 354 348 340 262 197 176
173 100 82 81
SnaBI TAC'GTA
Cuts at: 493 8230 8724 493
Size: 7737 494 2702
Fragments arranged by size:
7737 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10933
SphI G_CATG'C
Cuts at: 1132 3564 4592 6101 8291 1132
Size: 2432 1028 1509 2190 3774
Fragments arranged by size:
3774 2432 2190 1509 1028
SrfI GCCC'GGGC
Cuts at: 2136 2136
Size: 10933
SspI AAT'ATT
Cuts at: 5 52 2709 3408 4009 8857 5
Size: 47 2657 699 601 4848 2081
Fragments arranged by size:
4848 2657 2081 699 601 47
StuI AGG'CCT
Cuts at: 1517 6509 7106 1517
Size: 4992 597 5344
Fragments arranged by size:
5344 4992 597
StyI C'CwwG_G
Cuts at: 513 1065 2521 3996 6286 8372 513
Size: 552 1456 1475 2290 2086 3074
Fragments arranged by size:
3074 2290 2086 1475 1456 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2717 7340 8960 8977 9130 9315 9760
Size: 1255 1137 4623 1620 17 153 185 445
Cuts at: 9760 325
Size: 1498
Fragments arranged by size:
4623 1620 1498 1255 1137 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2562 3963 4518
Size: 274 80 33 51 1443 585 1401 555
Cuts at: 4518 4893 4915 6057 6819 7524 9179 10891 96
Size: 375 22 1142 762 705 1655 1712 138
Fragments arranged by size:
1712 1655 1443 1401 1142 762 705 585
555 375 274 138 80 51 33 22
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4308 4340 5868 5900 7748 7780 10893 10925 4308
Size: 32 1528 32 1848 32 3113 32 4316
Fragments arranged by size:
4316 3113 1848 1528 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2286 1316
Size: 970 9963
XbaI T'CTAG_A
Cuts at: 3772 8269 3772
Size: 4497 6436
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6899 6899
Size: 10933
XhoI C'TCGA_G
Cuts at: 1943 8147 1943
Size: 6204 4729
XmaI C'CCGG_G
Cuts at: 1870 2134 8258 1870
Size: 264 6124 4545
Fragments arranged by size:
6124 4545 264
XmnI GAAnn'nnTTC
Cuts at: 3284 3409 9062 3284
Size: 125 5653 5155
Fragments arranged by size:
5653 5155 125
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8741 276
Size: 53 83 186 936 7207 2468
Fragments arranged by size:
7207 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BsiWI DraIII
FseI FspAI NruI PacI PsrI RsrII SacII SanDI
SfiI SwaI