Restriction map for
pFN21ASDA1052
(Circular) (Six-base) MAPSORT of: pFN21ASDA1052.seq Check: 3706 from: 1 to: 9094
pFN21ASDA1052 9094 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3297 3637 3867 3297
Size: 340 230 8524
Fragments arranged by size:
8524 340 230
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2554 6904 278
Size: 53 83 186 936 1018 4350 2468
Fragments arranged by size:
4350 2468 1018 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6437 1937
Size: 4500 4594
Acc65I G'GTAC_C
Cuts at: 3432 6415 3432
Size: 2983 6111
AclI AA'CG_TT
Cuts at: 1563 7221 7594 1563
Size: 5658 373 3063
Fragments arranged by size:
5658 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3522 4955 5078 5101 5154 5609 7157 8541
Size: 1890 1433 123 23 53 455 1548 1384
Cuts at: 8541 8809 1632
Size: 268 1917
Fragments arranged by size:
1917 1890 1548 1433 1384 455 268 123
53 23
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8897
AflIII A'CryG_T
Cuts at: 1203 6159 6271 7993 8970 1203
Size: 4956 112 1722 977 1327
Fragments arranged by size:
4956 1722 1327 977 112
AgeI A'CCGG_T
Cuts at: 7925 7925
Size: 9094
AhdI GACnn_n'nnGTC
Cuts at: 3238 3379 5311 7823 3238
Size: 141 1932 2512 4509
Fragments arranged by size:
4509 2512 1932 141
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9094
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4553 6131 1184
Size: 3369 1578 4147
Fragments arranged by size:
4147 3369 1578
AlwNI CAG_nnn'CTG
Cuts at: 2846 3770 3800 3892 4126 4282 4906 4969 5104
Size: 924 30 92 234 156 624 63 135
Cuts at: 5104 5155 6837 8409 2846
Size: 51 1682 1572 3531
Fragments arranged by size:
3531 1682 1572 924 624 234 156 135
92 63 51 30
ApaI G_GGCC'C
Cuts at: 3475 3524 3683 4714 6033 3475
Size: 49 159 1031 1319 6536
Fragments arranged by size:
6536 1319 1031 159 49
ApaLI G'TGCA_C
Cuts at: 7151 8307 7151
Size: 1156 7938
ApoI r'AATT_y
Cuts at: 1493 2106 2114 5028 6403 6629 6781 8890 1493
Size: 613 8 2914 1375 226 152 2109 1697
Fragments arranged by size:
2914 2109 1697 1375 613 226 152 8
AseI AT'TA_AT
Cuts at: 160 1032 7648 160
Size: 872 6616 1606
Fragments arranged by size:
6616 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9094
AvaI C'yCGr_G
Cuts at: 1870 1943 2121 3524 3536 3839 5767 5916 6419
Size: 73 178 1403 12 303 1928 149 503
Cuts at: 6419 1870
Size: 4545
Fragments arranged by size:
4545 1928 1403 503 303 178 149 73
12
BamHI G'GATC_C
Cuts at: 3703 5666 6424 6791 3703
Size: 1963 758 367 6006
Fragments arranged by size:
6006 1963 758 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3432 3855 4677 4773 5331
Size: 325 217 627 1645 423 822 96 558
Cuts at: 5331 5445 5541 6415 7870 618
Size: 114 96 874 1455 1842
Fragments arranged by size:
1842 1645 1455 874 822 627 558 423
325 217 114 96 96
BanII G_rGCy'C
Cuts at: 1391 2537 2624 3475 3513 3524 3683 4057 4527
Size: 1146 87 851 38 11 159 374 470
Cuts at: 4527 4714 6033 6413 1391
Size: 187 1319 380 4072
Fragments arranged by size:
4072 1319 1146 851 470 380 374 187
159 87 38 11
BbeI G_GCGC'C
Cuts at: 4777 5449 4777
Size: 672 8422
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2942 3369 5259 928
Size: 971 1043 427 1890 4763
Fragments arranged by size:
4763 1890 1043 971 427
BbvCI CC'TCA_GC
Cuts at: 3584 4283 4644 5346 5843 3584
Size: 699 361 702 497 6835
Fragments arranged by size:
6835 702 699 497 361
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4226 4260 7285 7319 1903
Size: 34 2289 34 3025 34 3678
Fragments arranged by size:
3678 3025 2289 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2479 6986 8196 2479
Size: 4507 1210 3377
Fragments arranged by size:
4507 3377 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9094
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4856 7705 136
Size: 107 122 71 631 605 3184 2849 1525
Fragments arranged by size:
3184 2849 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5209 9089 5209
Size: 3880 5214
BlpI GC'TnA_GC
Cuts at: 4101 4347 4526 4665 5798 5831 6119 6511 4101
Size: 246 179 139 1133 33 288 392 6684
Fragments arranged by size:
6684 1133 392 288 246 179 139 33
Bme1580I G_kGCm'C
Cuts at: 1790 2764 3475 3524 3683 4680 4714 5657 6033
Size: 974 711 49 159 997 34 943 376
Cuts at: 6033 7155 8311 1790
Size: 1122 1156 2573
Fragments arranged by size:
2573 1156 1122 997 974 943 711 376
159 49 34
BmgBI CAC'GTC
Cuts at: 1318 5997 1318
Size: 4679 4415
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2432 2774 3308
Size: 416 53 83 399 24 1015 342 534
Cuts at: 3308 3362 6825 7783 442
Size: 54 3463 958 1753
Fragments arranged by size:
3463 1753 1015 958 534 416 399 342
83 54 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9094
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3501 3808 4414 3501
Size: 307 606 8181
Fragments arranged by size:
8181 606 307
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2220 2860 3443 3587 3732 4217 4349 4373
Size: 981 640 583 144 145 485 132 24
Cuts at: 4373 4384 4391 4715 4748 4787 4886 4991 5084
Size: 11 7 324 33 39 99 105 93
Cuts at: 5084 5147 5546 6161 7754 1239
Size: 63 399 615 1593 2579
Fragments arranged by size:
2579 1593 981 640 615 583 485 399
324 145 144 132 105 99 93 63
39 33 24 11 7
Bpu10I CC'TnA_GC
Cuts at: 3584 4056 4283 4620 4644 5346 5717 5843 3584
Size: 472 227 337 24 702 371 126 6835
Fragments arranged by size:
6835 702 472 371 337 227 126 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2573 2707 3991 4190 4781 7219 8084 8382 2573
Size: 134 1284 199 591 2438 865 298 3285
Fragments arranged by size:
3285 2438 1284 865 591 298 199 134
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2075 2388 2591 2618 4402 4874 5528
Size: 617 576 313 203 27 1784 472 654
Cuts at: 5528 7757 882
Size: 2229 2219
Fragments arranged by size:
2229 2219 1784 654 617 576 472 313
203 27
BsaAI yAC'GTr
Cuts at: 493 1204 4504 6391 6885 493
Size: 711 3300 1887 494 2702
Fragments arranged by size:
3300 2702 1887 711 494
BsaBI GATnn'nnATC
Cuts at: 9088 9088
Size: 9094
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2551 3165 4774 5446
Size: 53 83 186 936 1018 614 1609 672
Cuts at: 5446 6901 7283 7918 275
Size: 1455 382 635 1451
Fragments arranged by size:
1609 1455 1451 1018 936 672 635 614
382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4685 6255 7527 7925 8199 8346 8757 4685
Size: 1570 1272 398 274 147 411 5022
Fragments arranged by size:
5022 1570 1272 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2022 2052 3416 3446 3890
Size: 30 165 30 619 30 1364 30 444
Cuts at: 3890 3920 5939 5969 1178
Size: 30 2019 30 4303
Fragments arranged by size:
4303 2019 1364 619 444 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2696 2705 2801 2828 3104 3438 3440 3479
Size: 1510 9 96 27 276 334 2 39
Cuts at: 3479 3569 3686 3725 3929 3938 4013 4016 4019
Size: 90 117 39 204 9 75 3 3
Cuts at: 4019 4037 4357 4454 4591 4613 4661 4892 4988
Size: 18 320 97 137 22 48 231 96
Cuts at: 4988 5327 5615 5876 6034 1186
Size: 339 288 261 158 4246
Fragments arranged by size:
4246 1510 339 334 320 288 276 261
231 204 158 137 117 97 96 96
90 75 48 39 39 27 22 18
9 9 3 3 2
BseYI C'CCAG_C
Cuts at: 3342 3676 4446 4508 5433 5904 6151 6346 8297
Size: 334 770 62 925 471 247 195 1951
Cuts at: 8297 8793 3342
Size: 496 3643
Fragments arranged by size:
3643 1951 925 770 496 471 334 247
195 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2907 3786 4937 5405 1163
Size: 573 1171 879 1151 468 4852
Fragments arranged by size:
4852 1171 1151 879 573 468
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6544 7305 8333 1540
Size: 275 186 4543 761 1028 2301
Fragments arranged by size:
4543 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2537 3288 4057 4341 4479 4521 4527 4605 4992
Size: 751 769 284 138 42 6 78 387
Cuts at: 4992 5933 6363 7155 7240 8311 2537
Size: 941 430 792 85 1071 3320
Fragments arranged by size:
3320 1071 941 792 769 751 430 387
284 138 85 78 42 6
BsmI GAATG_Cn'
Cuts at: 3658 4258 6605 6698 3658
Size: 600 2347 93 6054
Fragments arranged by size:
6054 2347 600 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2537 2624 2764 3288 3475 3513 3524
Size: 399 747 87 140 524 187 38 11
Cuts at: 3524 3683 4057 4341 4479 4521 4527 4605 4680
Size: 159 374 284 138 42 6 78 75
Cuts at: 4680 4714 4992 5657 5933 6033 6363 6413 7155
Size: 34 278 665 276 100 330 50 742
Cuts at: 7155 7240 8311 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 747 742 665 524 399 374
330 284 278 276 187 159 140 138
100 87 85 78 75 50 42 38
34 11 6
BspEI T'CCGG_A
Cuts at: 8757 8757
Size: 9094
BspHI T'CATG_A
Cuts at: 6983 8713 6983
Size: 1730 7364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3297 3637 3867 4915 5592 6449 844
Size: 2453 340 230 1048 677 857 3489
Fragments arranged by size:
3489 2453 1048 857 677 340 230
BsrBI CCG'CTC
Cuts at: 1394 1823 2320 3469 3979 4357 5884 6981 1394
Size: 429 497 1149 510 378 1527 1097 3507
Fragments arranged by size:
3507 1527 1149 1097 510 497 429 378
BsrDI GCAATG_nn'
Cuts at: 65 1248 7589 7763 65
Size: 1183 6341 174 1396
Fragments arranged by size:
6341 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2849 4776 7738 7925 1700
Size: 110 1039 1927 2962 187 2869
Fragments arranged by size:
2962 2869 1927 1039 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9094
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9094
BssSI C'ACGA_G
Cuts at: 3461 4520 7154 8166 3461
Size: 1059 2634 1012 4389
Fragments arranged by size:
4389 2634 1059 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3892 4906 794
Size: 3098 1014 4982
Fragments arranged by size:
4982 3098 1014
BstBI TT'CG_AA
Cuts at: 2007 7898 2007
Size: 5891 3203
BstEII G'GTnAC_C
Cuts at: 1184 3004 6291 1184
Size: 1820 3287 3987
Fragments arranged by size:
3987 3287 1820
BstXI CCAn_nnnn'nTGG
Cuts at: 3421 5545 6139 3421
Size: 2124 594 6376
Fragments arranged by size:
6376 2124 594
BstYI r'GATC_y
Cuts at: 1972 2510 3276 3703 3927 5077 5209 5666 5787
Size: 538 766 427 224 1150 132 457 121
Cuts at: 5787 6424 6791 7176 7193 8645 8731 8743 9089
Size: 637 367 385 17 1452 86 12 346
Cuts at: 9089 1972
Size: 1977
Fragments arranged by size:
1977 1452 1150 766 637 538 457 427
385 367 346 224 132 121 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3708 5228 6213 3708
Size: 1520 985 6589
Fragments arranged by size:
6589 1520 985
BtgI C'CryG_G
Cuts at: 513 1065 2022 3247 3813 5791 513
Size: 552 957 1225 566 1978 3816
Fragments arranged by size:
3816 1978 1225 957 566 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8355
Fragments arranged by size:
8355 683 56
BtsI GCAGTG_nn'
Cuts at: 2192 2911 3037 3831 5728 5842 6610 7404 7424
Size: 719 126 794 1897 114 768 794 20
Cuts at: 7424 2192
Size: 3862
Fragments arranged by size:
3862 1897 794 794 768 719 126 114
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9094
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9059
DraI TTT'AAA
Cuts at: 6398 6398
Size: 9094
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8101 809
Size: 507 6785 1802
Fragments arranged by size:
6785 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3495 5457 5475
Size: 54 1425 50 275 18 1665 1962 18
Cuts at: 5475 5562 5961 6541 7430 9059 8
Size: 87 399 580 889 1629 43
Fragments arranged by size:
1962 1665 1629 1425 889 580 399 275
87 54 50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 6541 1537
Size: 275 4729 4090
Fragments arranged by size:
4729 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2508 2969 3102 3144 3501 3687 3813 3915
Size: 1155 461 133 42 357 186 126 102
Cuts at: 3915 4128 4206 4226 4931 5631 6305 6310 7024
Size: 213 78 20 705 700 674 5 714
Cuts at: 7024 1353
Size: 3423
Fragments arranged by size:
3423 1155 714 705 700 674 461 357
213 186 133 126 102 78 42 20
5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7681 8055 8201 387
Size: 1064 138 6092 374 146 1280
Fragments arranged by size:
6092 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2535 4055 4525 2535
Size: 1520 470 7104
Fragments arranged by size:
7104 1520 470
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2220 2860 3443 3522 3587 3732 4217
Size: 393 588 640 583 79 65 145 485
Cuts at: 4217 4349 4373 4384 4391 4715 4748 4787 4886
Size: 132 24 11 7 324 33 39 99
Cuts at: 4886 4955 4991 5078 5084 5101 5147 5154 5546
Size: 69 36 87 6 17 46 7 392
Cuts at: 5546 5609 6161 7157 7754 8541 8809 1239
Size: 63 552 996 597 787 268 1524
Fragments arranged by size:
1524 996 787 640 597 588 583 552
485 393 392 324 268 145 132 99
87 79 69 65 63 46 39 36
33 24 17 11 7 7 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2472 5528 2472
Size: 3056 6038
EcoO109I rG'GnC_Cy
Cuts at: 2458 3520 3521 3616 3680 4710 5224 5500 5920
Size: 1062 1 95 64 1030 514 276 420
Cuts at: 5920 6029 2458
Size: 109 5523
Fragments arranged by size:
5523 1062 1030 514 420 276 109 95
64 1
EcoRI G'AATT_C
Cuts at: 6403 6781 6403
Size: 378 8716
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2126 2632 5311 2126
Size: 506 2679 5909
Fragments arranged by size:
5909 2679 506
FspI TGC'GCA
Cuts at: 5466 5516 7600 5466
Size: 50 2084 6960
Fragments arranged by size:
6960 2084 50
HaeII r_GCGC'y
Cuts at: 1678 4552 4702 4777 5449 8241 1678
Size: 2874 150 75 672 2792 2531
Fragments arranged by size:
2874 2792 2531 672 150 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2022 2054 2166 2198 3224
Size: 32 135 32 450 32 112 32 1026
Cuts at: 3224 3256 3365 3397 3743 3775 4044 4076 4127
Size: 32 109 32 346 32 269 32 51
Cuts at: 4127 4159 5297 5329 5543 5575 7735 7767 7809
Size: 32 1138 32 214 32 2160 32 42
Cuts at: 7809 7841 1373
Size: 32 2626
Fragments arranged by size:
2626 2160 1138 1026 450 346 269 214
135 112 109 51 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2334 6438 6684 669
Size: 988 281 396 4104 246 3079
Fragments arranged by size:
4104 3079 988 396 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9094
HpaI GTT'AAC
Cuts at: 6684 6684
Size: 9094
KasI G'GCGC_C
Cuts at: 4773 5445 4773
Size: 672 8422
KpnI G_GTAC'C
Cuts at: 3436 6419 3436
Size: 2983 6111
MfeI C'AATT_G
Cuts at: 6693 6693
Size: 9094
MluI A'CGCG_T
Cuts at: 8970 8970
Size: 9094
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4119 4563 4797 5598 6057 8208 8392 1305
Size: 2814 444 234 801 459 2151 184 2007
Fragments arranged by size:
2814 2151 2007 801 459 444 234 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3497 5459 5477 5963 10
Size: 54 1425 343 1665 1962 18 486 3141
Fragments arranged by size:
3141 1962 1665 1425 486 343 54 18
MslI CAynn'nnrTG
Cuts at: 518 1166 2283 5547 7052 7411 7570 518
Size: 648 1117 3264 1505 359 159 2042
Fragments arranged by size:
3264 2042 1505 1117 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2056 3595 4282 4294 4450 4660 4906 4969
Size: 450 1539 687 12 156 210 246 63
Cuts at: 4969 5830 6115 6139 6346 6510 7187 8335 8580
Size: 861 285 24 207 164 677 1148 245
Cuts at: 8580 1606
Size: 2120
Fragments arranged by size:
2120 1539 1148 861 687 677 450 285
246 245 210 207 164 156 63 24
12
NaeI GCC'GGC
Cuts at: 4778 4778
Size: 9094
NarI GG'CG_CC
Cuts at: 4774 5446 4774
Size: 672 8422
NcoI C'CATG_G
Cuts at: 513 1065 2022 3247 3813 513
Size: 552 957 1225 566 5794
Fragments arranged by size:
5794 1225 957 566 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9094
NgoMIV G'CCGG_C
Cuts at: 4776 4776
Size: 9094
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9094
NotI GC'GGCC_GC
Cuts at: 6541 6541
Size: 9094
NspI r_CATG'y
Cuts at: 1132 1229 6163 6452 7997 1132
Size: 97 4934 289 1545 2229
Fragments arranged by size:
4934 2229 1545 289 97
PasI CC'CwG_GG
Cuts at: 1348 3517 3683 1348
Size: 2169 166 6759
Fragments arranged by size:
6759 2169 166
PciI A'CATG_T
Cuts at: 6159 7993 6159
Size: 1834 7260
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2198 3178 3253 4558 5455 6006 1108
Size: 1090 980 75 1305 897 551 4196
Fragments arranged by size:
4196 1305 1090 980 897 551 75
PfoI T'CCnGG_A
Cuts at: 3098 4803 4872 5850 3098
Size: 1705 69 978 6342
Fragments arranged by size:
6342 1705 978 69
PmeI GTTT'AAAC
Cuts at: 6398 6398
Size: 9094
PmlI CAC'GTG
Cuts at: 4504 4504
Size: 9094
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3195 3227 7163 7195 8676 8708 3195
Size: 32 3936 32 1481 32 3581
Fragments arranged by size:
3936 3581 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 5224 5920 5224
Size: 696 8398
PshAI GACnn'nnGTC
Cuts at: 1613 2895 1613
Size: 1282 7812
PsiI TTA'TAA
Cuts at: 6664 6664
Size: 9094
PspOMI G'GGCC_C
Cuts at: 3471 3520 3679 4710 6029 3471
Size: 49 159 1031 1319 6536
Fragments arranged by size:
6536 1319 1031 159 49
PspXI vC'TCGA_Gb
Cuts at: 3839 3839
Size: 9094
PstI C_TGCA'G
Cuts at: 830 3402 3577 3594 3987 4122 4278 4455 4707
Size: 2572 175 17 393 135 156 177 252
Cuts at: 4707 5046 5088 5109 6345 6446 830
Size: 339 42 21 1236 101 3478
Fragments arranged by size:
3478 2572 1236 393 339 252 177 175
156 135 101 42 21 17
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9094
PvuII CAG'CTG
Cuts at: 2056 3595 4282 4294 4450 4906 4969 5830 6346
Size: 1539 687 12 156 456 63 861 516
Cuts at: 6346 2056
Size: 4804
Fragments arranged by size:
4804 1539 861 687 516 456 156 63
12
SacI G_AGCT'C
Cuts at: 2537 4057 4527 2537
Size: 1520 470 7104
Fragments arranged by size:
7104 1520 470
SalI G'TCGA_C
Cuts at: 1936 6436 1936
Size: 4500 4594
SanDI GG'GwC_CC
Cuts at: 5920 5920
Size: 9094
SapI GCTCTTCn'nnn_
Cuts at: 2508 3501 4226 2508
Size: 993 725 7376
Fragments arranged by size:
7376 993 725
SbfI CC_TGCA'GG
Cuts at: 6446 6446
Size: 9094
ScaI AGT'ACT
Cuts at: 3837 7342 9054 3837
Size: 3505 1712 3877
Fragments arranged by size:
3877 3505 1712
SexAI A'CCwGG_T
Cuts at: 2944 2944
Size: 9094
SfcI C'TryA_G
Cuts at: 826 1045 3024 3398 3573 3590 3983 4118 4274
Size: 219 1979 374 175 17 393 135 156
Cuts at: 4274 4451 4703 5042 5084 5105 6188 6341 6442
Size: 177 252 339 42 21 1083 153 101
Cuts at: 6442 7577 8258 8449 826
Size: 1135 681 191 1471
Fragments arranged by size:
1979 1471 1135 1083 681 393 374 339
252 219 191 177 175 156 153 135
101 42 21 17
SfoI GGC'GCC
Cuts at: 4775 5447 4775
Size: 672 8422
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9094
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9094
SmaI CCC'GGG
Cuts at: 1872 2123 5918 6421 1872
Size: 251 3795 503 4545
Fragments arranged by size:
4545 3795 503 251
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2588 2722 3839 3970 4205 4760
Size: 197 926 645 134 1117 131 235 555
Cuts at: 4760 7198 8099 8361 820
Size: 2438 901 262 1553
Fragments arranged by size:
2438 1553 1117 926 901 645 555 262
235 197 134 131
SnaBI TAC'GTA
Cuts at: 493 6391 6885 493
Size: 5898 494 2702
Fragments arranged by size:
5898 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9094
SphI G_CATG'C
Cuts at: 1132 6452 1132
Size: 5320 3774
SspI AAT'ATT
Cuts at: 5 52 7018 5
Size: 47 6966 2081
Fragments arranged by size:
6966 2081 47
StuI AGG'CCT
Cuts at: 1517 3420 5642 1517
Size: 1903 2222 4969
Fragments arranged by size:
4969 2222 1903
StyI C'CwwG_G
Cuts at: 513 1065 2022 2418 3012 3117 3247 3813 4462
Size: 552 957 396 594 105 130 566 649
Cuts at: 4462 5239 5370 6533 513
Size: 777 131 1163 3074
Fragments arranged by size:
3074 1163 957 777 649 594 566 552
396 131 130 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3311 4973 5009 6007 6017 6328 7121
Size: 1255 1731 1662 36 998 10 311 793
Cuts at: 7121 7138 7291 7476 7921 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1731 1662 1498 1255 998 793 445 311
185 153 36 17 10
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3835 7340 9052
Size: 274 80 33 51 1443 1858 3505 1712
Cuts at: 9052 96
Size: 138
Fragments arranged by size:
3505 1858 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3890 3922 9054 9086 3890
Size: 32 5132 32 3898
Fragments arranged by size:
5132 3898 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2946 1316
Size: 1630 7464
XbaI T'CTAG_A
Cuts at: 6430 6430
Size: 9094
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2160 2410 3175 3769 2160
Size: 250 765 594 7485
Fragments arranged by size:
7485 765 594 250
XhoI C'TCGA_G
Cuts at: 1943 3839 1943
Size: 1896 7198
XmaI C'CCGG_G
Cuts at: 1870 2121 5916 6419 1870
Size: 251 3795 503 4545
Fragments arranged by size:
4545 3795 503 251
XmnI GAAnn'nnTTC
Cuts at: 5114 7223 5114
Size: 2109 6985
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2552 6902 276
Size: 53 83 186 936 1018 4350 2468
Fragments arranged by size:
4350 2468 1018 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI
PsrI RsrII SacII SfiI SrfI SwaI