Restriction map for
pFN21ASDA1051
(Circular) (Six-base) MAPSORT of: pFN21ASDA1051.seq Check: 2497 from: 1 to: 5800
pFN21ASDA1051 5800 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3610 278
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2535 3143 1937
Size: 598 608 4594
Fragments arranged by size:
4594 608 598
Acc65I G'GTAC_C
Cuts at: 2931 3003 3019 3121 2931
Size: 72 16 102 5610
Fragments arranged by size:
5610 102 72 16
AclI AA'CG_TT
Cuts at: 1563 3927 4300 1563
Size: 2364 373 3063
Fragments arranged by size:
3063 2364 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3863 5247 5515 1632
Size: 2231 1384 268 1917
Fragments arranged by size:
2231 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5603
AflIII A'CryG_T
Cuts at: 1203 2605 4699 5676 1203
Size: 1402 2094 977 1327
Fragments arranged by size:
2094 1402 1327 977
AgeI A'CCGG_T
Cuts at: 4631 4631
Size: 5800
AhdI GACnn_n'nnGTC
Cuts at: 4529 4529
Size: 5800
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5800
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5800
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2697 2729 2697
Size: 32 5768
AlwNI CAG_nnn'CTG
Cuts at: 3543 5115 3543
Size: 1572 4228
ApaI G_GGCC'C
Cuts at: 2201 2201
Size: 5800
ApaLI G'TGCA_C
Cuts at: 3857 5013 3857
Size: 1156 4644
ApoI r'AATT_y
Cuts at: 1493 2130 2815 2852 3109 3335 3487 5596 1493
Size: 637 685 37 257 226 152 2109 1697
Fragments arranged by size:
2109 1697 685 637 257 226 152 37
AseI AT'TA_AT
Cuts at: 160 1032 4354 160
Size: 872 3322 1606
Fragments arranged by size:
3322 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
AvaI C'yCGr_G
Cuts at: 1870 1943 3125 1870
Size: 73 1182 4545
Fragments arranged by size:
4545 1182 73
AvrII C'CTAG_G
Cuts at: 2299 2299
Size: 5800
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2987 3011 3020 3044 2987
Size: 24 9 24 5743
Fragments arranged by size:
5743 24 24 9
BamHI G'GATC_C
Cuts at: 3130 3497 3130
Size: 367 5433
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2181 2931 3003 3019 3121
Size: 325 217 627 394 750 72 16 102
Cuts at: 3121 4576 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 750 627 394 325 217 102
72 16
BanII G_rGCy'C
Cuts at: 1391 2201 2710 2767 3119 1391
Size: 810 509 57 352 4072
Fragments arranged by size:
4072 810 509 352 57
BbeI G_GCGC'C
Cuts at: 2185 2185
Size: 5800
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2829 928
Size: 971 930 3899
Fragments arranged by size:
3899 971 930
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2066 2099 2100 2133 3991 4025 1903
Size: 34 129 33 1 33 1858 34 3678
Fragments arranged by size:
3678 1858 129 34 34 33 33 1
BciVI GTATCCnnnnn_n'
Cuts at: 2459 2805 2867 3692 4902 2459
Size: 346 62 825 1210 3357
Fragments arranged by size:
3357 1210 825 346 62
BclI T'GATC_A
Cuts at: 1548 2104 2982 1548
Size: 556 878 4366
Fragments arranged by size:
4366 878 556
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4411 136
Size: 107 122 71 631 605 2739 1525
Fragments arranged by size:
2739 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5795 5795
Size: 5800
BlpI GC'TnA_GC
Cuts at: 2315 3217 2315
Size: 902 4898
Bme1580I G_kGCm'C
Cuts at: 1790 2201 3861 5017 1790
Size: 411 1660 1156 2573
Fragments arranged by size:
2573 1660 1156 411
BmgBI CAC'GTC
Cuts at: 1318 2533 1318
Size: 1215 4585
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2195 2592 2909
Size: 416 53 83 399 24 778 397 317
Cuts at: 2909 2930 3531 4489 442
Size: 21 601 958 1753
Fragments arranged by size:
1753 958 778 601 416 399 397 317
83 53 24 21
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2012 2502 2601 2702 4460 1239
Size: 773 490 99 101 1758 2579
Fragments arranged by size:
2579 1758 773 490 101 99
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3925 4790 5088 3925
Size: 865 298 4637
Fragments arranged by size:
4637 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4463 882
Size: 617 2964 2219
Fragments arranged by size:
2964 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3097 3591 493
Size: 711 1893 494 2702
Fragments arranged by size:
2702 1893 711 494
BsaBI GATnn'nnATC
Cuts at: 5794 5794
Size: 5800
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2182 3607 3989 4624
Size: 53 83 186 936 649 1425 382 635
Cuts at: 4624 275
Size: 1451
Fragments arranged by size:
1451 1425 936 649 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3016 4233 4631 4905 5052 5463 3016
Size: 1217 398 274 147 411 3353
Fragments arranged by size:
3353 1217 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2263 2293 2403 2433 2697
Size: 30 165 30 860 30 110 30 264
Cuts at: 2697 2727 1178
Size: 30 4251
Fragments arranged by size:
4251 860 264 165 110 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5800
BseYI C'CCAG_C
Cuts at: 5003 5499 5003
Size: 496 5304
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5227
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3250 4011 5039 1540
Size: 275 186 1249 761 1028 2301
Fragments arranged by size:
2301 1249 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2321 2348 2532 2587 2767 3861 3946 5017 2321
Size: 27 184 55 180 1094 85 1071 3104
Fragments arranged by size:
3104 1094 1071 184 180 85 55 27
BsmI GAATG_Cn'
Cuts at: 3311 3404 3311
Size: 93 5707
BsmBI CGTCTCn'nnnn_
Cuts at: 2940 2940
Size: 5800
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2201 2321 2348 2532 2587 2710 2767
Size: 399 411 120 27 184 55 123 57
Cuts at: 2767 3119 3861 3946 5017 1391
Size: 352 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 411 399 352 184 123
120 85 57 55 27
BspEI T'CCGG_A
Cuts at: 5463 5463
Size: 5800
BspHI T'CATG_A
Cuts at: 3689 5419 3689
Size: 1730 4070
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3155 844
Size: 2311 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3687 1394
Size: 429 1864 3507
Fragments arranged by size:
3507 1864 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4295 4469 65
Size: 1183 3047 174 1396
Fragments arranged by size:
3047 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4444 4631 1700
Size: 110 2634 187 2869
Fragments arranged by size:
2869 2634 187 110
BsrGI T'GTAC_A
Cuts at: 96 2511 96
Size: 2415 3385
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5800
BssSI C'ACGA_G
Cuts at: 3860 4872 3860
Size: 1012 4788
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5800
BstBI TT'CG_AA
Cuts at: 2007 4604 2007
Size: 2597 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5800
BstYI r'GATC_y
Cuts at: 1972 3130 3497 3882 3899 5351 5437 5449 5795
Size: 1158 367 385 17 1452 86 12 346
Cuts at: 5795 1972
Size: 1977
Fragments arranged by size:
1977 1452 1158 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5248
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2400 487
Size: 683 56 1174 3887
Fragments arranged by size:
3887 1174 683 56
BtsI GCAGTG_nn'
Cuts at: 2386 3316 4110 4130 2386
Size: 930 794 20 4056
Fragments arranged by size:
4056 930 794 20
ClaI AT'CG_AT
Cuts at: 1554 2076 1554
Size: 522 5278
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2145 2180 529
Size: 35 1581 35 4149
Fragments arranged by size:
4149 1581 35 35
DraI TTT'AAA
Cuts at: 3104 3104
Size: 5800
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4807 809
Size: 507 3491 1802
Fragments arranged by size:
3491 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2153 3247 4136
Size: 54 1425 50 275 18 323 1094 889
Cuts at: 4136 5765 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1094 889 323 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3247 1537
Size: 275 1435 4090
Fragments arranged by size:
4090 1435 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2646 2720 3730 1353
Size: 1293 74 1010 3423
Fragments arranged by size:
3423 1293 1010 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4387 4761 4907 387
Size: 1064 138 2798 374 146 1280
Fragments arranged by size:
2798 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2765 2765
Size: 5800
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2012 2502 2601 2702 3863 4460 5247
Size: 393 380 490 99 101 1161 597 787
Cuts at: 5247 5515 1239
Size: 268 1524
Fragments arranged by size:
1524 1161 787 597 490 393 380 268
101 99
EcoRI G'AATT_C
Cuts at: 2130 2852 3109 3487 2130
Size: 722 257 378 4443
Fragments arranged by size:
4443 722 378 257
FspI TGC'GCA
Cuts at: 4306 4306
Size: 5800
HaeII r_GCGC'y
Cuts at: 1678 2185 4947 1678
Size: 507 2762 2531
Fragments arranged by size:
2762 2531 507
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2058 2090 2243 2263 2275
Size: 32 135 32 486 32 153 20 12
Cuts at: 2275 2295 2343 2375 2386 2418 4441 4473 4515
Size: 20 48 32 11 32 2023 32 42
Cuts at: 4515 4547 1373
Size: 32 2626
Fragments arranged by size:
2626 2023 486 153 135 48 42 32
32 32 32 32 32 32 20 20
12 11
HincII GTy'rAC
Cuts at: 669 1657 1938 2233 2557 3144 3390 669
Size: 988 281 295 324 587 246 3079
Fragments arranged by size:
3079 988 587 324 295 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5800
HpaI GTT'AAC
Cuts at: 3390 3390
Size: 5800
KasI G'GCGC_C
Cuts at: 2181 2181
Size: 5800
KpnI G_GTAC'C
Cuts at: 2935 3007 3023 3125 2935
Size: 72 16 102 5610
Fragments arranged by size:
5610 102 72 16
MfeI C'AATT_G
Cuts at: 2172 3399 2172
Size: 1227 4573
MluI A'CGCG_T
Cuts at: 5676 5676
Size: 5800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2711 4914 5098 1305
Size: 1406 2203 184 2007
Fragments arranged by size:
2203 2007 1406 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2155 10
Size: 54 1425 343 323 3655
Fragments arranged by size:
3655 1425 343 323 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2112 2570 3758 4117 4276 518
Size: 648 946 458 1188 359 159 2042
Fragments arranged by size:
2042 1188 946 648 458 359 159
MspA1I CmG'CkG
Cuts at: 1606 3216 3893 5041 5286 1606
Size: 1610 677 1148 245 2120
Fragments arranged by size:
2120 1610 1148 677 245
NarI GG'CG_CC
Cuts at: 2182 2182
Size: 5800
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5248
NdeI CA'TA_TG
Cuts at: 387 2893 387
Size: 2506 3294
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5800
NotI GC'GGCC_GC
Cuts at: 3247 3247
Size: 5800
NspI r_CATG'y
Cuts at: 1132 1229 2609 3158 4703 1132
Size: 97 1380 549 1545 2229
Fragments arranged by size:
2229 1545 1380 549 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5800
PciI A'CATG_T
Cuts at: 2605 4699 2605
Size: 2094 3706
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2462 1108
Size: 1354 4446
PmeI GTTT'AAAC
Cuts at: 3104 3104
Size: 5800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2697 2729 3869 3901 5382 5414 2697
Size: 32 1140 32 1481 32 3083
Fragments arranged by size:
3083 1481 1140 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5800
PsiI TTA'TAA
Cuts at: 3370 3370
Size: 5800
PspOMI G'GGCC_C
Cuts at: 2197 2197
Size: 5800
PstI C_TGCA'G
Cuts at: 830 3152 830
Size: 2322 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5800
SacI G_AGCT'C
Cuts at: 2767 2767
Size: 5800
SalI G'TCGA_C
Cuts at: 1936 3142 1936
Size: 1206 4594
SapI GCTCTTCn'nnn_
Cuts at: 2646 2646
Size: 5800
SbfI CC_TGCA'GG
Cuts at: 3152 3152
Size: 5800
ScaI AGT'ACT
Cuts at: 4048 5760 4048
Size: 1712 4088
SfcI C'TryA_G
Cuts at: 826 1045 3148 4283 4964 5155 826
Size: 219 2103 1135 681 191 1471
Fragments arranged by size:
2103 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2183 2183
Size: 5800
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5800
SmaI CCC'GGG
Cuts at: 1872 3127 1872
Size: 1255 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3904 4805 5067 820
Size: 197 926 1961 901 262 1553
Fragments arranged by size:
1961 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3097 3591 493
Size: 2604 494 2702
Fragments arranged by size:
2702 2604 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5800
SphI G_CATG'C
Cuts at: 1132 3158 1132
Size: 2026 3774
SspI AAT'ATT
Cuts at: 5 52 3724 5
Size: 47 3672 2081
Fragments arranged by size:
3672 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5800
StyI C'CwwG_G
Cuts at: 513 1065 2299 3239 513
Size: 552 1234 940 3074
Fragments arranged by size:
3074 1234 940 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3827 3844 3997 4182 4627 325
Size: 1255 2247 17 153 185 445 1498
Fragments arranged by size:
2247 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2511 4046 5758
Size: 274 80 33 51 1443 534 1535 1712
Cuts at: 5758 96
Size: 138
Fragments arranged by size:
1712 1535 1443 534 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2311 2343 2403 2435 2977 3009 5760 5792 2311
Size: 32 60 32 542 32 2751 32 2319
Fragments arranged by size:
2751 2319 542 60 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2275 1316
Size: 959 4841
XbaI T'CTAG_A
Cuts at: 3136 3136
Size: 5800
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5800
XmaI C'CCGG_G
Cuts at: 1870 3125 1870
Size: 1255 4545
XmnI GAAnn'nnTTC
Cuts at: 3929 3929
Size: 5800
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3608 276
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BbvCI BfrBI BplI Bpu10I BsiWI
BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI
SexAI SfiI SrfI SwaI XcmI