Restriction map for
pFN21ASDA1048
(Circular) (Six-base) MAPSORT of: pFN21ASDA1048.seq Check: 5478 from: 1 to: 7318
pFN21ASDA1048 7318 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5128 278
Size: 53 83 186 936 3592 2468
Fragments arranged by size:
3592 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4100 4661 1937
Size: 2163 561 4594
Fragments arranged by size:
4594 2163 561
Acc65I G'GTAC_C
Cuts at: 4639 4639
Size: 7318
AclI AA'CG_TT
Cuts at: 1563 4077 5445 5818 1563
Size: 2514 1368 373 3063
Fragments arranged by size:
3063 2514 1368 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2573 4074 4167 4497 5381 6765 7033 1632
Size: 941 1501 93 330 884 1384 268 1917
Fragments arranged by size:
1917 1501 1384 941 884 330 268 93
AfeI AGC'GCT
Cuts at: 3183 3183
Size: 7318
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7121
AflIII A'CryG_T
Cuts at: 1203 6217 7194 1203
Size: 5014 977 1327
Fragments arranged by size:
5014 1327 977
AgeI A'CCGG_T
Cuts at: 6149 6149
Size: 7318
AhdI GACnn_n'nnGTC
Cuts at: 2156 6047 2156
Size: 3891 3427
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7318
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7318
AlwNI CAG_nnn'CTG
Cuts at: 2446 2915 3304 3379 3782 5061 6633 2446
Size: 469 389 75 403 1279 1572 3131
Fragments arranged by size:
3131 1572 1279 469 403 389 75
ApaLI G'TGCA_C
Cuts at: 5375 6531 5375
Size: 1156 6162
ApoI r'AATT_y
Cuts at: 1493 2624 3610 4627 4853 5005 7114 1493
Size: 1131 986 1017 226 152 2109 1697
Fragments arranged by size:
2109 1697 1131 1017 986 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5872 160
Size: 872 4840 1606
Fragments arranged by size:
4840 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7318
AvaI C'yCGr_G
Cuts at: 1870 1943 2104 4196 4643 1870
Size: 73 161 2092 447 4545
Fragments arranged by size:
4545 2092 447 161 73
BamHI G'GATC_C
Cuts at: 3233 4648 5015 3233
Size: 1415 367 5536
Fragments arranged by size:
5536 1415 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4639 6094 618
Size: 325 217 627 2852 1455 1842
Fragments arranged by size:
2852 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2075 2764 4637 1391
Size: 684 689 1873 4072
Fragments arranged by size:
4072 1873 689 684
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2128 928
Size: 971 229 6118
Fragments arranged by size:
6118 971 229
BbvCI CC'TCA_GC
Cuts at: 4005 4005
Size: 7318
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5509 5543 1903
Size: 34 3572 34 3678
Fragments arranged by size:
3678 3572 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5210 6420 5210
Size: 1210 6108
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7318
BfrBI ATG'CAT
Cuts at: 4522 4522
Size: 7318
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3044 5929 136
Size: 107 122 71 631 605 1372 2885 1525
Fragments arranged by size:
2885 1525 1372 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7313 7313
Size: 7318
BlpI GC'TnA_GC
Cuts at: 4213 4735 4213
Size: 522 6796
Bme1580I G_kGCm'C
Cuts at: 1790 2322 2981 3918 5379 6535 1790
Size: 532 659 937 1461 1156 2573
Fragments arranged by size:
2573 1461 1156 937 659 532
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7318
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3803 5049 6007
Size: 416 53 83 399 24 2386 1246 958
Cuts at: 6007 442
Size: 1753
Fragments arranged by size:
2386 1753 1246 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7318
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2065 2258 2462 3974 5978 1239
Size: 826 193 204 1512 2004 2579
Fragments arranged by size:
2579 2004 1512 826 204 193
Bpu10I CC'TnA_GC
Cuts at: 3387 4005 3387
Size: 618 6700
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2856 2951 3413 5443 6308 6606 2856
Size: 95 462 2030 865 298 3568
Fragments arranged by size:
3568 2030 865 462 298 95
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5981 882
Size: 617 4482 2219
Fragments arranged by size:
4482 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4104 4615 5109 493
Size: 711 2900 511 494 2702
Fragments arranged by size:
2900 2702 711 511 494
BsaBI GATnn'nnATC
Cuts at: 7312 7312
Size: 7318
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3356 5125 5507 6142
Size: 53 83 186 936 1823 1769 382 635
Cuts at: 6142 275
Size: 1451
Fragments arranged by size:
1823 1769 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5751 6149 6423 6570 6981 5751
Size: 398 274 147 411 6088
Fragments arranged by size:
6088 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3829 3859 4027 4057 1178
Size: 30 165 30 2426 30 168 30 4439
Fragments arranged by size:
4439 2426 168 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2103 3327 4047 1186
Size: 917 1224 720 4457
Fragments arranged by size:
4457 1224 917 720
BseYI C'CCAG_C
Cuts at: 3063 3137 3293 3405 3543 3747 3911 3981 6521
Size: 74 156 112 138 204 164 70 2540
Cuts at: 6521 7017 3063
Size: 496 3364
Fragments arranged by size:
3364 2540 496 204 164 156 138 112
74 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3969 1163
Size: 573 2233 4512
Fragments arranged by size:
4512 2233 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4768 5529 6557 1540
Size: 275 186 2767 761 1028 2301
Fragments arranged by size:
2767 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2391 2478 2704 5379 5464 6535 2391
Size: 87 226 2675 85 1071 3174
Fragments arranged by size:
3174 2675 1071 226 87 85
BsmI GAATG_Cn'
Cuts at: 4829 4922 4829
Size: 93 7225
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2075 2322 2391 2478 2704 2764 2981
Size: 399 285 247 69 87 226 60 217
Cuts at: 2981 3918 4637 5379 5464 6535 1391
Size: 937 719 742 85 1071 2174
Fragments arranged by size:
2174 1071 937 742 719 399 285 247
226 217 87 85 69 60
BspEI T'CCGG_A
Cuts at: 6981 6981
Size: 7318
BspHI T'CATG_A
Cuts at: 5207 6937 5207
Size: 1730 5588
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2146 4673 844
Size: 1302 2527 3489
Fragments arranged by size:
3489 2527 1302
BsrBI CCG'CTC
Cuts at: 1394 1823 3386 5205 1394
Size: 429 1563 1819 3507
Fragments arranged by size:
3507 1819 1563 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2227 5813 5987 65
Size: 1183 979 3586 174 1396
Fragments arranged by size:
3586 1396 1183 979 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3475 5962 6149 1700
Size: 110 1665 2487 187 2869
Fragments arranged by size:
2869 2487 1665 187 110
BsrGI T'GTAC_A
Cuts at: 96 2721 2992 96
Size: 2625 271 4422
Fragments arranged by size:
4422 2625 271
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7318
BssSI C'ACGA_G
Cuts at: 5378 6390 5378
Size: 1012 6306
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3604 794
Size: 2810 4508
BstBI TT'CG_AA
Cuts at: 2007 6122 2007
Size: 4115 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7318
BstXI CCAn_nnnn'nTGG
Cuts at: 3911 3911
Size: 7318
BstYI r'GATC_y
Cuts at: 1972 2308 3085 3233 3872 4456 4648 5015 5400
Size: 336 777 148 639 584 192 367 385
Cuts at: 5400 5417 6869 6955 6967 7313 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 777 639 584 385 367 346
336 192 148 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3222 3222
Size: 7318
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6766
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6579
Fragments arranged by size:
6579 683 56
BtsI GCAGTG_nn'
Cuts at: 2122 2881 4834 5628 5648 2122
Size: 759 1953 794 20 3792
Fragments arranged by size:
3792 1953 794 759 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7318
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7283
DraI TTT'AAA
Cuts at: 4622 4622
Size: 7318
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6325 809
Size: 507 5009 1802
Fragments arranged by size:
5009 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2416 3663 4765
Size: 54 1425 50 275 18 586 1247 1102
Cuts at: 4765 5654 7283 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1247 1102 889 586 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4765 1537
Size: 275 2953 4090
Fragments arranged by size:
4090 2953 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2125 3191 3428 4371 4431 5248 1353
Size: 772 1066 237 943 60 817 3423
Fragments arranged by size:
3423 1066 943 817 772 237 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2195 5905 6279 6425 387
Size: 1064 138 606 3710 374 146 1280
Fragments arranged by size:
3710 1280 1064 606 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2065 2258 2462 2573 3974 4074 4167
Size: 393 433 193 204 111 1401 100 93
Cuts at: 4167 4497 5381 5978 6765 7033 1239
Size: 330 884 597 787 268 1524
Fragments arranged by size:
1524 1401 884 787 597 433 393 330
268 204 193 111 100 93
EcoNI CCTnn'n_nnAGG
Cuts at: 4092 4092
Size: 7318
EcoO109I rG'GnC_Cy
Cuts at: 2548 3961 2548
Size: 1413 5905
EcoRI G'AATT_C
Cuts at: 4627 5005 4627
Size: 378 6940
FspI TGC'GCA
Cuts at: 5824 5824
Size: 7318
HaeII r_GCGC'y
Cuts at: 1678 2446 2616 3185 6465 1678
Size: 768 170 569 3280 2531
Fragments arranged by size:
3280 2531 768 569 170
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2142 2174 4115 4147 5959
Size: 32 135 32 570 32 1941 32 1812
Cuts at: 5959 5991 6033 6065 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1941 1812 570 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2257 2716 4564 4662 4908 669
Size: 988 281 319 459 1848 98 246 3079
Fragments arranged by size:
3079 1848 988 459 319 281 246 98
HindIII A'AGCT_T
Cuts at: 748 3017 4175 4202 748
Size: 2269 1158 27 3864
Fragments arranged by size:
3864 2269 1158 27
HpaI GTT'AAC
Cuts at: 4564 4908 4564
Size: 344 6974
KpnI G_GTAC'C
Cuts at: 4643 4643
Size: 7318
MfeI C'AATT_G
Cuts at: 3111 3555 4917 3111
Size: 444 1362 5512
Fragments arranged by size:
5512 1362 444
MluI A'CGCG_T
Cuts at: 7194 7194
Size: 7318
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2883 3850 6432 6616 1305
Size: 1578 967 2582 184 2007
Fragments arranged by size:
2582 2007 1578 967 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2418 3665 10
Size: 54 1425 343 586 1247 3663
Fragments arranged by size:
3663 1425 1247 586 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2396 5276 5635 5794 518
Size: 648 1230 2880 359 159 2042
Fragments arranged by size:
2880 2042 1230 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2085 3050 3661 3688 3761 3985 3998 4004
Size: 479 965 611 27 73 224 13 6
Cuts at: 4004 4145 4217 4734 5411 6559 6804 1606
Size: 141 72 517 677 1148 245 2120
Fragments arranged by size:
2120 1148 965 677 611 517 479 245
224 141 73 72 27 13 6
NaeI GCC'GGC
Cuts at: 3477 3477
Size: 7318
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6766
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7318
NgoMIV G'CCGG_C
Cuts at: 3475 3475
Size: 7318
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7318
NotI GC'GGCC_GC
Cuts at: 4765 4765
Size: 7318
NsiI A_TGCA'T
Cuts at: 4524 4524
Size: 7318
NspI r_CATG'y
Cuts at: 1132 1229 2251 2881 4676 6221 1132
Size: 97 1022 630 1795 1545 2229
Fragments arranged by size:
2229 1795 1545 1022 630 97
PasI CC'CwG_GG
Cuts at: 1348 3373 4573 1348
Size: 2025 1200 4093
Fragments arranged by size:
4093 2025 1200
PciI A'CATG_T
Cuts at: 6217 6217
Size: 7318
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4573 1108
Size: 3465 3853
PmeI GTTT'AAAC
Cuts at: 4622 4622
Size: 7318
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5387 5419 6900 6932 5387
Size: 32 1481 32 5773
Fragments arranged by size:
5773 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2548 3961 2548
Size: 1413 5905
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7318
PsiI TTA'TAA
Cuts at: 4083 4888 4083
Size: 805 6513
PstI C_TGCA'G
Cuts at: 830 3232 3766 4003 4670 830
Size: 2402 534 237 667 3478
Fragments arranged by size:
3478 2402 667 534 237
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7318
PvuII CAG'CTG
Cuts at: 3050 3661 3688 3761 3985 3998 4004 4145 4217
Size: 611 27 73 224 13 6 141 72
Cuts at: 4217 3050
Size: 6151
Fragments arranged by size:
6151 611 224 141 73 72 27 13
6
SalI G'TCGA_C
Cuts at: 1936 4660 1936
Size: 2724 4594
SanDI GG'GwC_CC
Cuts at: 3961 3961
Size: 7318
SbfI CC_TGCA'GG
Cuts at: 4670 4670
Size: 7318
ScaI AGT'ACT
Cuts at: 5566 7278 5566
Size: 1712 5606
SexAI A'CCwGG_T
Cuts at: 2937 2937
Size: 7318
SfcI C'TryA_G
Cuts at: 826 1045 3228 3762 3999 4666 5801 6482 6673
Size: 219 2183 534 237 667 1135 681 191
Cuts at: 6673 826
Size: 1471
Fragments arranged by size:
2183 1471 1135 681 667 534 237 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7318
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7318
SmaI CCC'GGG
Cuts at: 1872 4645 1872
Size: 2773 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2871 2966 3428 5422 6323 6585
Size: 197 926 928 95 462 1994 901 262
Cuts at: 6585 820
Size: 1553
Fragments arranged by size:
1994 1553 928 926 901 462 262 197
95
SnaBI TAC'GTA
Cuts at: 493 4615 5109 493
Size: 4122 494 2702
Fragments arranged by size:
4122 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7318
SphI G_CATG'C
Cuts at: 1132 4676 1132
Size: 3544 3774
SspI AAT'ATT
Cuts at: 5 52 2329 5242 5
Size: 47 2277 2913 2081
Fragments arranged by size:
2913 2277 2081 47
StuI AGG'CCT
Cuts at: 1517 3130 1517
Size: 1613 5705
StyI C'CwwG_G
Cuts at: 513 1065 3580 4249 4602 4757 513
Size: 552 2515 669 353 155 3074
Fragments arranged by size:
3074 2515 669 552 353 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2595 3307 5345 5362 5515 5700 6145
Size: 1255 1015 712 2038 17 153 185 445
Cuts at: 6145 325
Size: 1498
Fragments arranged by size:
2038 1498 1255 1015 712 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2721 2992 3529
Size: 274 80 33 51 1443 744 271 537
Cuts at: 3529 5564 7276 96
Size: 2035 1712 138
Fragments arranged by size:
2035 1712 1443 744 537 274 271 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2218 2250 7278 7310 2218
Size: 32 5028 32 2226
Fragments arranged by size:
5028 2226 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7318
XbaI T'CTAG_A
Cuts at: 4654 4654
Size: 7318
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7318
XmaI C'CCGG_G
Cuts at: 1870 4643 1870
Size: 2773 4545
XmnI GAAnn'nnTTC
Cuts at: 2033 5447 2033
Size: 3414 3904
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5126 276
Size: 53 83 186 936 3592 2468
Fragments arranged by size:
3592 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NarI NruI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SapI SfiI SfoI
SrfI SwaI XcmI