Restriction map for
pFN21ASDA1045
(Circular) (Six-base) MAPSORT of: pFN21ASDA1045.seq Check: 8362 from: 1 to: 5929
pFN21ASDA1045 5929 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2050 2050
Size: 5929
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3739 278
Size: 53 83 186 936 2203 2468
Fragments arranged by size:
2468 2203 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3272 1937
Size: 1335 4594
Acc65I G'GTAC_C
Cuts at: 3250 3250
Size: 5929
AclI AA'CG_TT
Cuts at: 1563 4056 4429 1563
Size: 2493 373 3063
Fragments arranged by size:
3063 2493 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3230 3992 5376 5644 1632
Size: 1598 762 1384 268 1917
Fragments arranged by size:
1917 1598 1384 762 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5732
AflIII A'CryG_T
Cuts at: 1203 4828 5805 1203
Size: 3625 977 1327
Fragments arranged by size:
3625 1327 977
AgeI A'CCGG_T
Cuts at: 4760 4760
Size: 5929
AhdI GACnn_n'nnGTC
Cuts at: 2827 4658 2827
Size: 1831 4098
AleI CACnn'nnGTG
Cuts at: 1166 2583 1166
Size: 1417 4512
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5929
AlwNI CAG_nnn'CTG
Cuts at: 2469 3672 5244 2469
Size: 1203 1572 3154
Fragments arranged by size:
3154 1572 1203
ApaI G_GGCC'C
Cuts at: 2740 2980 2740
Size: 240 5689
ApaLI G'TGCA_C
Cuts at: 3986 5142 3986
Size: 1156 4773
ApoI r'AATT_y
Cuts at: 1493 3238 3464 3616 5725 1493
Size: 1745 226 152 2109 1697
Fragments arranged by size:
2109 1745 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4483 160
Size: 872 3451 1606
Fragments arranged by size:
3451 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5929
AvaI C'yCGr_G
Cuts at: 1870 1943 2916 2934 3254 1870
Size: 73 973 18 320 4545
Fragments arranged by size:
4545 973 320 73 18
BamHI G'GATC_C
Cuts at: 3259 3626 3259
Size: 367 5562
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3250 4705 618
Size: 325 217 627 1463 1455 1842
Fragments arranged by size:
1842 1463 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2174 2391 2740 2917 2980 3248 1391
Size: 783 217 349 177 63 268 4072
Fragments arranged by size:
4072 783 349 268 217 177 63
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4958
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2199 2233 4120 4154 1903
Size: 34 262 34 1887 34 3678
Fragments arranged by size:
3678 1887 262 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3215 3821 5031 3215
Size: 606 1210 4113
Fragments arranged by size:
4113 1210 606
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5929
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2508 2930 4540
Size: 107 122 71 631 605 836 422 1610
Cuts at: 4540 136
Size: 1525
Fragments arranged by size:
1610 1525 836 631 605 422 122 107
71
BglII A'GATC_T
Cuts at: 5924 5924
Size: 5929
BlpI GC'TnA_GC
Cuts at: 3346 3346
Size: 5929
Bme1580I G_kGCm'C
Cuts at: 1790 2740 2980 3990 5146 1790
Size: 950 240 1010 1156 2573
Fragments arranged by size:
2573 1156 1010 950 240
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5929
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3660 4618 442
Size: 416 53 83 399 24 2243 958 1753
Fragments arranged by size:
2243 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5929
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2355 2405 2590 2702 4589 1239
Size: 1116 50 185 112 1887 2579
Fragments arranged by size:
2579 1887 1116 185 112 50
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4054 4919 5217 4054
Size: 865 298 4766
Fragments arranged by size:
4766 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2630 4592 882
Size: 617 1131 1962 2219
Fragments arranged by size:
2219 1962 1131 617
BsaAI yAC'GTr
Cuts at: 493 1204 3226 3720 493
Size: 711 2022 494 2702
Fragments arranged by size:
2702 2022 711 494
BsaBI GATnn'nnATC
Cuts at: 5923 5923
Size: 5929
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3736 4118 4753 275
Size: 53 83 186 936 2203 382 635 1451
Fragments arranged by size:
2203 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4362 4760 5034 5181 5592 4362
Size: 398 274 147 411 4699
Fragments arranged by size:
4699 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2606 2636 1178
Size: 30 165 30 1203 30 4471
Fragments arranged by size:
4471 1203 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2207 2309 2627 2762 1186
Size: 1021 102 318 135 4353
Fragments arranged by size:
4353 1021 318 135 102
BseYI C'CCAG_C
Cuts at: 2979 3067 5132 5628 2979
Size: 88 2065 496 3280
Fragments arranged by size:
3280 2065 496 88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2084 2455 2482 2550 1163
Size: 573 348 371 27 68 4542
Fragments arranged by size:
4542 573 371 348 68 27
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2248 3379 4140 5168 1540
Size: 275 186 247 1131 761 1028 2301
Fragments arranged by size:
2301 1131 1028 761 275 247 186
BsiHKAI G_wGCw'C
Cuts at: 2951 3990 4075 5146 2951
Size: 1039 85 1071 3734
Fragments arranged by size:
3734 1071 1039 85
BsmI GAATG_Cn'
Cuts at: 3440 3533 3440
Size: 93 5836
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2174 2391 2740 2917 2951 2980 3248
Size: 399 384 217 349 177 34 29 268
Cuts at: 3248 3990 4075 5146 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 384 349 268 217
177 85 34 29
BspEI T'CCGG_A
Cuts at: 5592 5592
Size: 5929
BspHI T'CATG_A
Cuts at: 3818 5548 3818
Size: 1730 4199
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2050 3284 844
Size: 1206 1234 3489
Fragments arranged by size:
3489 1234 1206
BsrBI CCG'CTC
Cuts at: 1394 1823 2263 2905 3816 1394
Size: 429 440 642 911 3507
Fragments arranged by size:
3507 911 642 440 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4424 4598 65
Size: 1183 3176 174 1396
Fragments arranged by size:
3176 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2243 4573 4760 1700
Size: 110 433 2330 187 2869
Fragments arranged by size:
2869 2330 433 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5929
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5929
BssSI C'ACGA_G
Cuts at: 3989 5001 3989
Size: 1012 4917
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2469 2860 794
Size: 1675 391 3863
Fragments arranged by size:
3863 1675 391
BstBI TT'CG_AA
Cuts at: 2007 4733 2007
Size: 2726 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5929
BstXI CCAn_nnnn'nTGG
Cuts at: 3133 3133
Size: 5929
BstYI r'GATC_y
Cuts at: 1972 3259 3626 4011 4028 5480 5566 5578 5924
Size: 1287 367 385 17 1452 86 12 346
Cuts at: 5924 1972
Size: 1977
Fragments arranged by size:
1977 1452 1287 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2292 2292
Size: 5929
BtgI C'CryG_G
Cuts at: 513 1065 2022 2155 513
Size: 552 957 133 4287
Fragments arranged by size:
4287 957 552 133
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3037 487
Size: 683 56 1811 3379
Fragments arranged by size:
3379 1811 683 56
BtsI GCAGTG_nn'
Cuts at: 2947 3031 3445 4239 4259 2947
Size: 84 414 794 20 4617
Fragments arranged by size:
4617 794 414 84 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5929
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5894
DraI TTT'AAA
Cuts at: 3233 3233
Size: 5929
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4936 809
Size: 507 3620 1802
Fragments arranged by size:
3620 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2245 3376 4265
Size: 54 1425 50 275 18 415 1131 889
Cuts at: 4265 5894 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1131 889 415 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2245 3376 1537
Size: 275 433 1131 4090
Fragments arranged by size:
4090 1131 433 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2226 2459 2751 3859 1353
Size: 873 233 292 1108 3423
Fragments arranged by size:
3423 1108 873 292 233
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2327 4516 4890 5036 387
Size: 1064 138 738 2189 374 146 1280
Fragments arranged by size:
2189 1280 1064 738 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2355 2405 2590 2702 3230 3992 4589
Size: 393 723 50 185 112 528 762 597
Cuts at: 4589 5376 5644 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 762 723 597 528 393 268
185 112 50
EcoNI CCTnn'n_nnAGG
Cuts at: 2744 2744
Size: 5929
EcoO109I rG'GnC_Cy
Cuts at: 2148 2736 2737 3047 2148
Size: 588 1 310 5030
Fragments arranged by size:
5030 588 310 1
EcoRI G'AATT_C
Cuts at: 3238 3616 3238
Size: 378 5551
FspI TGC'GCA
Cuts at: 4435 4435
Size: 5929
HaeII r_GCGC'y
Cuts at: 1678 5076 1678
Size: 3398 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2813 2845 3107 3139 4570
Size: 32 135 32 1241 32 262 32 1431
Cuts at: 4570 4602 4644 4676 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1431 1241 262 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2407 3273 3519 669
Size: 988 281 469 866 246 3079
Fragments arranged by size:
3079 988 866 469 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5929
HpaI GTT'AAC
Cuts at: 3519 3519
Size: 5929
KpnI G_GTAC'C
Cuts at: 3254 3254
Size: 5929
MfeI C'AATT_G
Cuts at: 3528 3528
Size: 5929
MluI A'CGCG_T
Cuts at: 5805 5805
Size: 5929
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2149 5043 5227 1305
Size: 844 2894 184 2007
Fragments arranged by size:
2894 2007 844 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4107
Fragments arranged by size:
4107 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2583 3887 4246 4405 518
Size: 648 861 556 1304 359 159 2042
Fragments arranged by size:
2042 1304 861 648 556 359 159
MspA1I CmG'CkG
Cuts at: 1606 2444 2534 2647 3345 4022 5170 5415 1606
Size: 838 90 113 698 677 1148 245 2120
Fragments arranged by size:
2120 1148 838 698 677 245 113 90
NaeI GCC'GGC
Cuts at: 2245 2245
Size: 5929
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4420
Fragments arranged by size:
4420 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5929
NgoMIV G'CCGG_C
Cuts at: 2243 2243
Size: 5929
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5929
NotI GC'GGCC_GC
Cuts at: 3376 3376
Size: 5929
NspI r_CATG'y
Cuts at: 1132 1229 3287 4832 1132
Size: 97 2058 1545 2229
Fragments arranged by size:
2229 2058 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5929
PciI A'CATG_T
Cuts at: 4828 4828
Size: 5929
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5929
PfoI T'CCnGG_A
Cuts at: 2178 2226 2178
Size: 48 5881
PmeI GTTT'AAAC
Cuts at: 3233 3233
Size: 5929
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3998 4030 5511 5543 3998
Size: 32 1481 32 4384
Fragments arranged by size:
4384 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2148 3047 2148
Size: 899 5030
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5929
PsiI TTA'TAA
Cuts at: 3499 3499
Size: 5929
PspOMI G'GGCC_C
Cuts at: 2736 2976 2736
Size: 240 5689
PstI C_TGCA'G
Cuts at: 830 2856 3026 3281 830
Size: 2026 170 255 3478
Fragments arranged by size:
3478 2026 255 170
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5929
PvuII CAG'CTG
Cuts at: 2444 2444
Size: 5929
SalI G'TCGA_C
Cuts at: 1936 3271 1936
Size: 1335 4594
SanDI GG'GwC_CC
Cuts at: 2148 3047 2148
Size: 899 5030
SapI GCTCTTCn'nnn_
Cuts at: 2751 2751
Size: 5929
SbfI CC_TGCA'GG
Cuts at: 3281 3281
Size: 5929
ScaI AGT'ACT
Cuts at: 4177 5889 4177
Size: 1712 4217
SfcI C'TryA_G
Cuts at: 826 1045 2163 2852 3022 3277 4412 5093 5284
Size: 219 1118 689 170 255 1135 681 191
Cuts at: 5284 826
Size: 1471
Fragments arranged by size:
1471 1135 1118 689 681 255 219 191
170
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5929
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5929
SmaI CCC'GGG
Cuts at: 1872 3256 1872
Size: 1384 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4033 4934 5196 820
Size: 197 926 2090 901 262 1553
Fragments arranged by size:
2090 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3226 3720 493
Size: 2733 494 2702
Fragments arranged by size:
2733 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5929
SphI G_CATG'C
Cuts at: 1132 3287 1132
Size: 2155 3774
SspI AAT'ATT
Cuts at: 5 52 3853 5
Size: 47 3801 2081
Fragments arranged by size:
3801 2081 47
StuI AGG'CCT
Cuts at: 1517 2116 1517
Size: 599 5330
StyI C'CwwG_G
Cuts at: 513 1065 2022 2518 3368 513
Size: 552 957 496 850 3074
Fragments arranged by size:
3074 957 850 552 496
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3956 3973 4126 4311 4756 325
Size: 1255 2376 17 153 185 445 1498
Fragments arranged by size:
2376 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4175 5887 96
Size: 274 80 33 51 1443 2198 1712 138
Fragments arranged by size:
2198 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2205 2237 5889 5921 2205
Size: 32 3652 32 2213
Fragments arranged by size:
3652 2213 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5929
XbaI T'CTAG_A
Cuts at: 3265 3265
Size: 5929
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2350 2350
Size: 5929
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5929
XmaI C'CCGG_G
Cuts at: 1870 3254 1870
Size: 1384 4545
XmnI GAAnn'nnTTC
Cuts at: 4058 4058
Size: 5929
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3737 276
Size: 53 83 186 936 2203 2468
Fragments arranged by size:
2468 2203 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SacII SexAI SfiI
SfoI SrfI SwaI